RLG00000000598

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005917
Physical Location & Seq
Reverse (-)
2424241 .. 2426220
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000598

Sequence Viewer

Length: 1980 bp
ATGGCAAATGCAGTATTCTCGTTCACACAATTTGATTTCGATATCCAAGGCGGTTTGAGTCCCCCCCAGCTTGAAGATTATGACAAAGAAGAAACAGTAATGTTCAATGTTGGCAAGGGAGACCATTTGTTTGGACTAGAAGATCAGTTGGGAGGAATGAGTACTCCTTCTTCTGAACATGGTTTCTACCAAGACAACAACGCCAAGACAGGAGTAGTAGTATGCCATTTGTCCAAAGATCAAAACCAACACCGTGATCTTGAACACCAACAGCAGCAACAGCAATCCAAGACAGACTTTTTGATTCTGGATAACTTCAACTTCGAGACTGTTTCTTCTCAACATGGTTTTTATCATGCCAATAAGGTGGGGAAGGATCACCAACACCACCATTACCAACAACAGCAACAACAATCCAAGTCAAGTTTTTCAATTTTGGAGAATTTCAATAACTTTGATTTTTACTTCCCCTCGGGACAAGTTACAGAACCAGTTGAGGATGGTGCTCGGTTTACACACAGCCAGATCAGAGGCTCAGACATTTTCGAAACGAACAAGCAATTGTCGCTTCCTCAATCTTCTCTAGGAACACTGGAGCTCCTTAACAGTTACGGAAGCGCCTTCAAGAAGTTAAAGGGAGAAAGACAAAGCAGTAGCAGGGCAGTTAAGGGTAATGAAATTGATCAAAACAGCAGGTACGTTGTTAATGGTAATCAGAAACTGTCCACCGAAGAGGTCATGAGGGTTGCTGGAGCAAGGTATGTGCAATTTTCTAGTCAGGGGTATGCAAATTGTTACTTGTCTATGCACCCTTTTGGCTATGCGCTTTCAGGTCTCTGCGAAGAAGAAACGAAAGATGTAGAACTTGTTCACTTACTATTAGCTGCAGCAGAGAAAGTAGGATACCAACAATTTGAAAGAGCAAGCAGATTGCTTCTGCAGTGTGAATGGATTCCATCCTTCAAAGGTACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCAGAGGCACTTCGTGAGAGGATCGAAAAAGAAACAGGACAGTATACATCTAAGGGAAATGAAGAATTGTCTCAATATGCACATGGTTTGGGGACAAACCTTGCATACATCGCATTCCACCAAGAAGTCCCTTTCCATCCCGTACTGCAGTTCGCGGCAATCCAAGCCATGATCGAAAATGTTGCAAATGAGAGTAGGATCCATTTGATTGATCTGGAAATCAGGAGTGGTGTGCAATGGACAGGGTGGATGGAAGCAATTGCAGAACGAGAGGAATGCCCGGTAGAGCTTCTTACTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGGCAAGTGTGGCAAAGTCCTTGAACCTACCCTTTAAATTTAAGGAAGTTATTGTGTCAGACATGCAAGACATTAATAACCAGTTATTCGAAGAAATTGAAGATGATGAAGTTGTGGTTGTGTACGCGCCATTAATACTGAGGACAATGATAGCAAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATATCAACCCTTTTTTGATGGTGGTCATTGAAGTGGAAGCTAATCTTAATTCCCCATCATTTGTGAACCGTTTCATTGATGCATTGTTTTTTTATGGTGCATTTTTCGACTGCCTTGAAACCTGTATGAAACAGGAGAAGTACAGGGTGATCATGGAAGGCTTATTGCGTGAGGGAATCCGGAACATAGTGGCGGCAGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCAAGGTTTAGCATGGTGGAAATGAGCTTTAGTAACTCATCATTTTATCAAGCTAGTCTGATGGCTAAAAAGTTCTGTTGCCCCACACTTGATAGCAATGAAAAATGTCTCACTGTTGGGTGGAAGGGAACCCCAATTCATTCGCTTTCCGCTTGGAAGTTCCGTCTTGAAAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

660

Amino Acids

75.07

Weight (kDa)

5.59

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 289 - 653 2.2e-77 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 684
AccI GTMKAC 1 cut(s) 1043
AccII CGCG 2 cut(s) 1154, 1478
AccIII TCCGGA 1 cut(s) 1725
AciI CCGC 4 cut(s) 51, 1154, 1739, 1950
AclWI GGATC 4 cut(s) 384, 1028, 1192, 1205
AcsI RAATTY 2 cut(s) 442, 1388
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 6 cut(s) 163, 698, 970, 1143, 1475, 1688
AluBI AGCT 7 cut(s) 70, 598, 884, 1288, 1586, 1827, 1853
AluI AGCT 7 cut(s) 70, 598, 884, 1288, 1586, 1827, 1853
Alw21I GWGCWC 2 cut(s) 508, 600
Alw26I GTCTC 6 cut(s) 114, 320, 839, 1074, 1331, 1913
AlwI GGATC 4 cut(s) 384, 1028, 1192, 1205
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 472
Aor13HI TCCGGA 1 cut(s) 1725
AoxI GGCC 2 cut(s) 1508, 1791
ApeKI GCWGC 3 cut(s) 274, 884, 887
ApoI RAATTY 2 cut(s) 442, 1388
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 283, 315
AseI ATTAAT 2 cut(s) 1425, 1484
Asp700I GAANNNNTTC 3 cut(s) 867, 951, 1616
AspA2I CCTAGG 1 cut(s) 1511
AspLEI GCGC 3 cut(s) 620, 826, 1480
AspS9I GGNCC 1 cut(s) 1791
AsuC2I CCSGG 1 cut(s) 1280
AsuHPI GGTGA 3 cut(s) 371, 1549, 1705
AsuII TTCGAA 2 cut(s) 546, 1440
AvaI CYCGRG 1 cut(s) 472
AvrII CCTAGG 1 cut(s) 1511
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BamHI GGATCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 600
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
Bbv12I GWGCWC 2 cut(s) 508, 600
BbvI GCAGC 3 cut(s) 286, 871, 899
BccI CCATC 8 cut(s) 494, 964, 1143, 1243, 1558, 1609, 1771, 1855
BcgI CGANNNNNNTGC 5 cut(s) 34, 1163, 1197, 1257, 1291
BciVI GTATCC 1 cut(s) 896
BclI TGATCA 2 cut(s) 682, 1695
BcnI CCSGG 1 cut(s) 1280
BcoDI GTCTC 6 cut(s) 114, 320, 839, 1074, 1331, 1913
BfaI CTAG 5 cut(s) 137, 584, 774, 1512, 1854
BfmI CTRYAG 3 cut(s) 885, 938, 1145
BfoI RGCGCY 1 cut(s) 621
BfuAI ACCTGC 1 cut(s) 684
BfuI GTATCC 1 cut(s) 896
BisI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
BlnI CCTAGG 1 cut(s) 1511
BlsI GCNGC 5 cut(s) 276, 886, 889, 1156, 1741
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 1280
BmeT110I CYCGRG 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 1791
BmiI GGNNCC 2 cut(s) 1199, 1930
BmrFI CCNGG 1 cut(s) 1280
BmsI GCATC 1 cut(s) 1615
BpmI CTGGAG 2 cut(s) 614, 771
Bpu14I TTCGAA 2 cut(s) 546, 1440
BpuMI CCSGG 1 cut(s) 1280
BsaI GGTCTC 2 cut(s) 114, 839
BsaJI CCNNGG 3 cut(s) 46, 471, 1511
BsaWI WCCGGW 1 cut(s) 1725
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 582, 612
Bse1I ACTGG 3 cut(s) 491, 597, 1432
Bse3DI GCAATG 2 cut(s) 1241, 1903
BseAI TCCGGA 1 cut(s) 1725
BseDI CCNNGG 3 cut(s) 46, 471, 1511
BseGI GGATG 5 cut(s) 505, 956, 1135, 1254, 1786
BseMI GCAATG 2 cut(s) 1241, 1903
BseMII CTCAG 2 cut(s) 549, 1481
BseNI ACTGG 3 cut(s) 491, 597, 1432
BseXI GCAGC 3 cut(s) 286, 871, 899
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 1154, 1478
BshFI GGCC 2 cut(s) 1510, 1793
BsiHKAI GWGCWC 2 cut(s) 508, 600
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 2 cut(s) 1280, 1726
BslFI GGGAC 4 cut(s) 45, 489, 1105, 1112
BsmAI GTCTC 6 cut(s) 114, 320, 839, 1074, 1331, 1913
BsmFI GGGAC 4 cut(s) 45, 489, 1105, 1112
BsmI GAATGC 2 cut(s) 1112, 1280
BsnI GGCC 2 cut(s) 1510, 1793
Bso31I GGTCTC 2 cut(s) 114, 839
BsoBI CYCGRG 1 cut(s) 472
Bsp119I TTCGAA 2 cut(s) 546, 1440
Bsp1286I GDGCHC 2 cut(s) 508, 600
Bsp13I TCCGGA 1 cut(s) 1725
BspACI CCGC 4 cut(s) 51, 1154, 1739, 1950
BspANI GGCC 2 cut(s) 1510, 1793
BspCNI CTCAG 2 cut(s) 548, 1482
BspEI TCCGGA 1 cut(s) 1725
BspFNI CGCG 2 cut(s) 1154, 1478
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 2 cut(s) 1199, 1930
BspMAI CTGCAG 3 cut(s) 889, 942, 1149
BspMI ACCTGC 1 cut(s) 684
BspPI GGATC 4 cut(s) 384, 1028, 1192, 1205
BspT104I TTCGAA 2 cut(s) 546, 1440
BspTNI GGTCTC 2 cut(s) 114, 839
BsrDI GCAATG 2 cut(s) 1241, 1903
BsrI ACTGG 3 cut(s) 491, 597, 1432
BssECI CCNNGG 3 cut(s) 46, 471, 1511
BssNAI GTATAC 1 cut(s) 1044
BssT1I CCWWGG 2 cut(s) 46, 1511
Bst1107I GTATAC 1 cut(s) 1044
Bst4CI ACNGT 8 cut(s) 97, 254, 331, 608, 723, 1041, 1616, 1915
Bst6I CTCTTC 1 cut(s) 726
BstBI TTCGAA 2 cut(s) 546, 1440
BstC8I GCNNGC 1 cut(s) 925
BstDEI CTNAG 3 cut(s) 535, 1050, 1490
BstF5I GGATG 5 cut(s) 505, 956, 1135, 1254, 1786
BstFNI CGCG 2 cut(s) 1154, 1478
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 620, 826, 1480
BstMAI GTCTC 6 cut(s) 114, 320, 839, 1074, 1331, 1913
BstMWI GCNNNNNNNGC 6 cut(s) 280, 565, 1109, 1163, 1361, 1799
BstNSI RCATGY 1 cut(s) 1417
BstSCI CCNGG 1 cut(s) 1278
BstSFI CTRYAG 3 cut(s) 885, 938, 1145
BstUI CGCG 2 cut(s) 1154, 1478
BstV1I GCAGC 3 cut(s) 286, 871, 899
BstX2I RGATCY 1 cut(s) 1197
BstXI CCANNNNNNTGG 2 cut(s) 131, 367
BstYI RGATCY 1 cut(s) 1197
BstZ17I GTATAC 1 cut(s) 1044
BsuI GTATCC 1 cut(s) 896
BsuRI GGCC 2 cut(s) 1510, 1793
BtgZI GCGATG 1 cut(s) 1093
BtsCI GGATG 5 cut(s) 505, 956, 1135, 1254, 1786
BtsI GCAGTG 1 cut(s) 947
BtsIMutI CAGTG 3 cut(s) 590, 947, 1911
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 1 cut(s) 925
CaiI CAGNNNCTG 1 cut(s) 721
CciI TCATGA 1 cut(s) 738
CfoI GCGC 3 cut(s) 620, 826, 1480
Cfr13I GGNCC 1 cut(s) 1791
Csp6I GTAC 6 cut(s) 162, 697, 969, 1142, 1474, 1687
CviAII CATG 9 cut(s) 179, 344, 356, 739, 1082, 1168, 1414, 1699, 1813
CviQI GTAC 6 cut(s) 162, 697, 969, 1142, 1474, 1687
DdeI CTNAG 3 cut(s) 535, 1050, 1490
DraI TTTAAA 1 cut(s) 1387
DraIII CACNNNGTG 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 46, 1511
Eco147I AGGCCT 1 cut(s) 1510
Eco24I GRGCYC 1 cut(s) 600
Eco31I GGTCTC 2 cut(s) 114, 839
Eco32I GATATC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 1791
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 46, 1511
EcoT22I ATGCAT 1 cut(s) 1630
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 2 cut(s) 46, 1511
FaeI CATG 9 cut(s) 182, 347, 359, 742, 1085, 1171, 1417, 1702, 1816
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 1575, 1607
FaqI GGGAC 4 cut(s) 45, 489, 1105, 1112
FatI CATG 9 cut(s) 178, 343, 355, 738, 1081, 1167, 1413, 1698, 1812
FbaI TGATCA 2 cut(s) 682, 1695
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
FokI GGATG 5 cut(s) 512, 943, 1122, 1261, 1793
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
FspBI CTAG 5 cut(s) 137, 584, 774, 1512, 1854
GlaI GCGC 3 cut(s) 619, 825, 1479
GluI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 614, 771
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 2 cut(s) 1510, 1793
HapII CCGG 2 cut(s) 1280, 1726
HhaI GCGC 3 cut(s) 620, 826, 1480
Hin1II CATG 9 cut(s) 182, 347, 359, 742, 1085, 1171, 1417, 1702, 1816
Hin6I GCGC 3 cut(s) 618, 824, 1478
HinP1I GCGC 3 cut(s) 618, 824, 1478
HinfI GANTC 4 cut(s) 58, 304, 952, 1722
HpaII CCGG 2 cut(s) 1280, 1726
HphI GGTGA 3 cut(s) 371, 1549, 1705
Hpy166II GTNNAC 7 cut(s) 24, 513, 726, 871, 1044, 1474, 1612
Hpy188I TCNGA 7 cut(s) 175, 530, 538, 717, 981, 1411, 1860
Hpy8I GTNNAC 7 cut(s) 24, 513, 726, 871, 1044, 1474, 1612
HpyAV CCTTC 8 cut(s) 177, 367, 631, 970, 1697, 1739, 1804, 1918
HpyCH4III ACNGT 8 cut(s) 97, 254, 331, 608, 723, 1041, 1616, 1915
HpyCH4IV ACGT 1 cut(s) 699
HpyF10VI GCNNNNNNNGC 6 cut(s) 280, 565, 1109, 1163, 1361, 1799
HpyF3I CTNAG 3 cut(s) 535, 1050, 1490
HpySE526I ACGT 1 cut(s) 699
Hsp92II CATG 9 cut(s) 182, 347, 359, 742, 1085, 1171, 1417, 1702, 1816
HspAI GCGC 3 cut(s) 618, 824, 1478
Kpn2I TCCGGA 1 cut(s) 1725
Ksp22I TGATCA 2 cut(s) 682, 1695
LmnI GCTCC 3 cut(s) 595, 603, 752
Lsp1109I GCAGC 3 cut(s) 286, 871, 899
LweI GCATC 1 cut(s) 1615
MaeI CTAG 5 cut(s) 137, 584, 774, 1512, 1854
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 4 cut(s) 481, 608, 794, 1832
MfeI CAATTG 2 cut(s) 560, 1257
MflI RGATCY 1 cut(s) 1197
MhlI GDGCHC 2 cut(s) 508, 600
MlyI GAGTC 1 cut(s) 67
Mph1103I ATGCAT 1 cut(s) 1630
MroI TCCGGA 1 cut(s) 1725
MroXI GAANNNNTTC 3 cut(s) 867, 951, 1616
MseI TTAA 9 cut(s) 603, 632, 666, 705, 1386, 1392, 1425, 1484, 1593
MslI CAYNNNNRTG 1 cut(s) 1577
MspI CCGG 2 cut(s) 1280, 1726
MspR9I CCNGG 1 cut(s) 1280
MunI CAATTG 2 cut(s) 560, 1257
Mva1269I GAATGC 2 cut(s) 1112, 1280
MvnI CGCG 2 cut(s) 1154, 1478
MwoI GCNNNNNNNGC 6 cut(s) 280, 565, 1109, 1163, 1361, 1799
NciI CCSGG 1 cut(s) 1280
NlaIII CATG 9 cut(s) 182, 347, 359, 742, 1085, 1171, 1417, 1702, 1816
NlaIV GGNNCC 2 cut(s) 1199, 1930
NsiI ATGCAT 1 cut(s) 1630
NspI RCATGY 1 cut(s) 1417
NspV TTCGAA 2 cut(s) 546, 1440
PagI TCATGA 1 cut(s) 738
PceI AGGCCT 1 cut(s) 1510
PctI GAATGC 2 cut(s) 1112, 1280
PdmI GAANNNNTTC 3 cut(s) 867, 951, 1616
PfeI GAWTC 3 cut(s) 304, 952, 1722
PkrI GCNGC 5 cut(s) 276, 886, 889, 1156, 1741
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PshBI ATTAAT 2 cut(s) 1425, 1484
Psp124BI GAGCTC 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 1199, 1930
PspPI GGNCC 1 cut(s) 1791
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 3 cut(s) 889, 942, 1149
PstNI CAGNNNCTG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 1197
RsaI GTAC 6 cut(s) 163, 698, 970, 1143, 1475, 1688
RsaNI GTAC 6 cut(s) 162, 697, 969, 1142, 1474, 1687
RseI CAYNNNNRTG 1 cut(s) 1577
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 9 cut(s) 603, 632, 666, 705, 1386, 1392, 1425, 1484, 1593
SatI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
Sau96I GGNCC 1 cut(s) 1791
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 1280
SduI GDGCHC 2 cut(s) 508, 600
SfaNI GCATC 1 cut(s) 1615
SfcI CTRYAG 3 cut(s) 885, 938, 1145
SfuI TTCGAA 2 cut(s) 546, 1440
SmiMI CAYNNNNRTG 1 cut(s) 1577
SseBI AGGCCT 1 cut(s) 1510
SsiI CCGC 4 cut(s) 51, 1154, 1739, 1950
SspMI CTAG 5 cut(s) 137, 584, 774, 1512, 1854
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 1 cut(s) 1510
StyD4I CCNGG 1 cut(s) 1278
StyI CCWWGG 2 cut(s) 46, 1511
TaaI ACNGT 8 cut(s) 97, 254, 331, 608, 723, 1041, 1616, 1915
TaiI ACGT 1 cut(s) 702
TaqI TCGA 8 cut(s) 39, 324, 546, 1023, 1173, 1440, 1653, 1765
TatI WGTACW 2 cut(s) 161, 1686
TauI GCSGC 2 cut(s) 1157, 1742
TfiI GAWTC 3 cut(s) 304, 952, 1722
Tru1I TTAA 9 cut(s) 603, 632, 666, 705, 1386, 1392, 1425, 1484, 1593
Tru9I TTAA 9 cut(s) 603, 632, 666, 705, 1386, 1392, 1425, 1484, 1593
TscAI CASTG 3 cut(s) 597, 947, 1918
TseI GCWGC 3 cut(s) 274, 884, 887
TspDTI ATGAA 7 cut(s) 690, 1074, 1473, 1609, 1688, 1914, 1928
TspGWI ACGGA 2 cut(s) 627, 1952
TspRI CASTG 3 cut(s) 597, 947, 1918
VspI ATTAAT 2 cut(s) 1425, 1484
XapI RAATTY 2 cut(s) 442, 1388
XceI RCATGY 1 cut(s) 1417
XmaJI CCTAGG 1 cut(s) 1511
XmiI GTMKAC 1 cut(s) 1043
XmnI GAANNNNTTC 3 cut(s) 867, 951, 1616
XspI CTAG 5 cut(s) 137, 584, 774, 1512, 1854
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 1630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.