MD03G1293700.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005917
Physical Location & Seq
Forward (+)
37023448 .. 37025172
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1293700.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGAAAAGTTATCTCATTTGTCCAAAGATCAACAACAATCCAAGGCAAGTTATTTGATGTTGGATAATTTCAGTTTCGATACAGGTTGTTCCGAACATGATCTCCAACGTTCCAGCAGCAAGTGCGAGAAAGCACGATCTCATGTTTCCAAAGATCACGACCACCGCCACTACCAACAACAAAATCAACATTACCAACAACCATCCAAGTCAAATTACTCCGATTGGGATAATTTCACCTTTGACTCTGCCTTCTCATCCACGCAACCAATCCAGGACGCGACGAATGAGCTCACGCACAGCCAAATAGGAGAATCAGACATTGCGGAGACCAACAAGCAAATGCCCCATCAGTCTTCCTTAGCTGTGTTGGAGCTCCTAAACAACTACGGCAGTGCGTTCAAGAATCTGAGGGGAGACAGATTAAGCAACAGATGCAATGAAGCTGAAGCCTCCTTGTTTTCGATCCAACAAAAATTGTCAACCGAGGAGATCATGAGGGTTGCCGGAGCAAGGTATGTGCAATTCTCTAATCAAGGATATGGCGATTTTTTCATGCCTTTGCACCCGTTTGGCTACGCGCTTTCGGGTCTATCTGAAGAAGAAACGAAAGATGTGGAGCTTGCTCACATCTTTTTAGCTGCAGCAGAGAAGGTAGGCTACCAACAATTCGAGCGCGCAAACCGCCTGCTTCTTCGTTGCGAATTTGCTGCCTCCTTCAAAGTCAACCCTGTCCAGAGGGTTGTTTTCTATTTTGCCAAGGCGCTTCGCGAGAGGATCGACAACGAAACGGGGAACAGCATGTATAAGGGGAATGAAATGTCTACTTATAGTAATGGACTAAGCACAAACCTTACAACTCTTGCATTCTACCAAAATACCCCTTTTCATTCAGTTATGCAGTACCCAGGAATCCAAGTAATACTCGAAAATGTAGTCTTGGAAAGCAAGGTTCATTTGATTGATCTCGAAATCAGATGCGGAGTGCAGTGGACAGGGCTGATGGAAGTGCTTGCAGGGCGCGAGGAGTGCCCCATTGAGCTCCTTACAATAACTGCAGTGGGAGTGACGGGCAAGCAGAAGATCGAGGAGACAGGAAGGAGGCTGGCAAGCGTCGCAGAGGCCTTGAATTTACCCTTTCAATTCAAGGCAGTGATTGTGACAGACATGGAAGATGTCAAGGACCAATTGTTTGATGTCAAGGATGACGAAGCTGTGGTGGTTTACGCGCCTATAATACTAAGGACAATGATTTCGAGGCCAAGTTGCTTGGAAAATCTGATGAGGGTGATGAGAAAGCTCACCCCTTGTGTAATGGTGGTGATTGAGGTGGAGGCCAACCACAATTCACCGTCATTTGTGAACCGATTCATCGATGCGCTGTTTTATTACAGTGCATTTTTCGACTGCCTTGAGACTTGCATGAAGCAGGAGAAGGACAGAGTGCTGATGGAGGGGTTGTTACATGAGGGAATACGAAACATTGTGGTGGCGGAGGGGAGTGAAAGGGTTGTGAGAAGCGTGACGATGGAGGTGTGGAGAGCCTTCTTTGCAAGGCTTAGAATTGTGGAAATCAACTTAAGTAATGTAGCTTTGTACCAAGCTAGTTTGGTGGCTAAAAAGTTTGGAAGCCCTCCTTGCACACTTGATAGGAATGGGAAATGTCTTATTGTTGGGTGGAAAGGAACCCCAATCCATTCACTTTCGGCTTGGAAGTTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

575

Amino Acids

65.18

Weight (kDa)

5.94

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 209 - 572 4.6e-80 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 824
AccII CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
AciI CCGC 5 cut(s) 166, 326, 685, 981, 1493
AclI AACGTT 1 cut(s) 109
AclWI GGATC 2 cut(s) 460, 785
AcsI RAATTY 2 cut(s) 704, 1129
AcuI CTGAAG 2 cut(s) 468, 618
AfaI GTAC 2 cut(s) 905, 1598
AfiI CCNNNNNNNGG 1 cut(s) 513
AflII CTTAAG 1 cut(s) 1579
AgsI TTSAA 5 cut(s) 403, 721, 1129, 1142, 1147
AjnI CCWGG 2 cut(s) 273, 907
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
Alw21I GWGCWC 3 cut(s) 294, 378, 1044
Alw26I GTCTC 4 cut(s) 323, 411, 1085, 1409
AlwI GGATC 2 cut(s) 460, 785
AoxI GGCC 3 cut(s) 1122, 1259, 1335
ApeKI GCWGC 4 cut(s) 117, 641, 644, 710
ApoI RAATTY 2 cut(s) 704, 1129
ArsI GACNNNNNNTTYG 6 cut(s) 921, 953, 1175, 1207, 1237, 1269
Asp700I GAANNNNTTC 1 cut(s) 1367
AspLEI GCGC 7 cut(s) 583, 678, 680, 766, 1023, 1231, 1381
AspS9I GGNCC 1 cut(s) 1183
AsuHPI GGTGA 5 cut(s) 229, 1294, 1300, 1333, 1341
AvaII GGWCC 1 cut(s) 1183
BaeGI GKGCMC 1 cut(s) 1034
BanII GRGCYC 3 cut(s) 294, 378, 1044
BarI GAAGNNNNNNTAC 2 cut(s) 644, 676
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 3 cut(s) 294, 378, 1044
BbvI GCAGC 4 cut(s) 129, 628, 656, 697
BccI CCATC 5 cut(s) 211, 357, 997, 1444, 1522
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 2 cut(s) 275, 909
BcoDI GTCTC 4 cut(s) 323, 411, 1085, 1409
BfaI CTAG 1 cut(s) 1605
BfmI CTRYAG 2 cut(s) 642, 1056
BfoI RGCGCY 1 cut(s) 767
BfrI CTTAAG 1 cut(s) 1579
BisI GCNGC 4 cut(s) 118, 642, 645, 711
BlsI GCNGC 4 cut(s) 119, 643, 646, 712
Bme1390I CCNGG 2 cut(s) 275, 909
Bme18I GGWCC 1 cut(s) 1183
BmgT120I GGNCC 1 cut(s) 1183
BmiI GGNNCC 1 cut(s) 1687
BmrFI CCNGG 2 cut(s) 275, 909
BmsI GCATC 3 cut(s) 425, 968, 1366
BpiI GAAGAC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 361
BpuEI CTTGAG 1 cut(s) 1433
Bsa29I ATCGAT 1 cut(s) 1374
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 4 cut(s) 42, 486, 759, 907
BsaXI ACNNNNNCTCC 4 cut(s) 87, 117, 1445, 1475
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse3DI GCAATG 2 cut(s) 321, 445
BseBI CCWGG 2 cut(s) 275, 909
BseCI ATCGAT 1 cut(s) 1374
BseDI CCNNGG 4 cut(s) 42, 486, 759, 907
BseGI GGATG 3 cut(s) 203, 257, 1210
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 2 cut(s) 321, 445
BseMII CTCAG 1 cut(s) 401
BsePI GCGCGC 1 cut(s) 676
BseRI GAGGAG 3 cut(s) 503, 1040, 1103
BseSI GKGCMC 1 cut(s) 1034
BseXI GCAGC 4 cut(s) 129, 628, 656, 697
BsgI GTGCAG 1 cut(s) 1007
Bsh1236I CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
BshFI GGCC 3 cut(s) 1124, 1261, 1337
BshVI ATCGAT 1 cut(s) 1374
BsiHKAI GWGCWC 3 cut(s) 294, 378, 1044
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 4 cut(s) 323, 411, 1085, 1409
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 3 cut(s) 1124, 1261, 1337
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 4 cut(s) 294, 378, 1034, 1044
Bsp143I GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
Bsp68I TCGCGA 1 cut(s) 771
BspACI CCGC 5 cut(s) 166, 326, 685, 981, 1493
BspANI GGCC 3 cut(s) 1124, 1261, 1337
BspCNI CTCAG 1 cut(s) 402
BspDI ATCGAT 1 cut(s) 1374
BspFNI CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 1687
BspMAI CTGCAG 2 cut(s) 646, 1060
BspPI GGATC 2 cut(s) 460, 785
BspTI CTTAAG 1 cut(s) 1579
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 321, 445
BssECI CCNNGG 4 cut(s) 42, 486, 759, 907
BssHII GCGCGC 1 cut(s) 676
BssMI GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
BssT1I CCWWGG 2 cut(s) 42, 759
Bst2UI CCWGG 2 cut(s) 275, 909
Bst4CI ACNGT 2 cut(s) 1353, 1394
BstAFI CTTAAG 1 cut(s) 1579
BstAPI GCANNNNNTGC 2 cut(s) 123, 435
BstC8I GCNNGC 7 cut(s) 624, 678, 689, 1014, 1076, 1107, 1111
BstDEI CTNAG 5 cut(s) 361, 410, 842, 1241, 1559
BstF5I GGATG 3 cut(s) 203, 257, 1210
BstFNI CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
BstH2I RGCGCY 1 cut(s) 767
BstHHI GCGC 7 cut(s) 583, 678, 680, 766, 1023, 1231, 1381
BstKTI GATC 9 cut(s) 31, 103, 140, 157, 468, 495, 780, 967, 1086
BstMAI GTCTC 4 cut(s) 323, 411, 1085, 1409
BstMBI GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
BstMWI GCNNNNNNNGC 8 cut(s) 123, 435, 684, 1018, 1029, 1115, 1550, 1638
BstNI CCWGG 2 cut(s) 275, 909
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 2 cut(s) 273, 907
BstSFI CTRYAG 2 cut(s) 642, 1056
BstSLI GKGCMC 1 cut(s) 1034
BstUI CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
BstV1I GCAGC 4 cut(s) 129, 628, 656, 697
BstV2I GAAGAC 1 cut(s) 348
Bsu15I ATCGAT 1 cut(s) 1374
BsuRI GGCC 3 cut(s) 1124, 1261, 1337
BsuTUI ATCGAT 1 cut(s) 1374
BtsCI GGATG 3 cut(s) 203, 257, 1210
BtsI GCAGTG 4 cut(s) 400, 995, 1065, 1158
BtsIMutI CAGTG 5 cut(s) 400, 995, 1065, 1158, 1399
BtuMI TCGCGA 1 cut(s) 771
Cac8I GCNNGC 7 cut(s) 624, 678, 689, 1014, 1076, 1107, 1111
CciI TCATGA 1 cut(s) 495
CfoI GCGC 7 cut(s) 583, 678, 680, 766, 1023, 1231, 1381
Cfr13I GGNCC 1 cut(s) 1183
ClaI ATCGAT 1 cut(s) 1374
CseI GACGC 2 cut(s) 287, 1102
Csp6I GTAC 2 cut(s) 904, 1597
CviAII CATG 8 cut(s) 98, 143, 496, 556, 802, 1168, 1423, 1466
CviQI GTAC 2 cut(s) 904, 1597
DdeI CTNAG 5 cut(s) 361, 410, 842, 1241, 1559
DpnI GATC 9 cut(s) 30, 102, 139, 156, 467, 494, 779, 966, 1085
DpnII GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
EciI GGCGGA 1 cut(s) 1508
Ecl136II GAGCTC 3 cut(s) 292, 376, 1042
Eco130I CCWWGG 2 cut(s) 42, 759
Eco147I AGGCCT 1 cut(s) 1124
Eco24I GRGCYC 3 cut(s) 294, 378, 1044
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 1183
Eco53kI GAGCTC 3 cut(s) 292, 376, 1042
Eco57I CTGAAG 2 cut(s) 468, 618
EcoICRI GAGCTC 3 cut(s) 292, 376, 1042
EcoRII CCWGG 2 cut(s) 273, 907
EcoT14I CCWWGG 2 cut(s) 42, 759
EcoT38I GRGCYC 3 cut(s) 294, 378, 1044
ErhI CCWWGG 2 cut(s) 42, 759
FaeI CATG 8 cut(s) 101, 146, 499, 559, 805, 1171, 1426, 1469
FalI AAGNNNNNCTT 2 cut(s) 1621, 1653
FatI CATG 8 cut(s) 97, 142, 495, 555, 801, 1167, 1422, 1465
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 4 cut(s) 118, 642, 645, 711
FokI GGATG 3 cut(s) 190, 244, 1217
FriOI GRGCYC 3 cut(s) 294, 378, 1044
Fsp4HI GCNGC 4 cut(s) 118, 642, 645, 711
FspBI CTAG 1 cut(s) 1605
GlaI GCGC 7 cut(s) 582, 677, 679, 765, 1022, 1230, 1380
GluI GCNGC 4 cut(s) 118, 642, 645, 711
HaeII RGCGCY 1 cut(s) 767
HaeIII GGCC 3 cut(s) 1124, 1261, 1337
HapII CCGG 1 cut(s) 507
HgaI GACGC 2 cut(s) 287, 1102
HhaI GCGC 7 cut(s) 583, 678, 680, 766, 1023, 1231, 1381
Hin1II CATG 8 cut(s) 101, 146, 499, 559, 805, 1171, 1426, 1469
Hin6I GCGC 7 cut(s) 581, 676, 678, 764, 1021, 1229, 1379
HinP1I GCGC 7 cut(s) 581, 676, 678, 764, 1021, 1229, 1379
HincII GTYRAC 2 cut(s) 483, 727
HindII GTYRAC 2 cut(s) 483, 727
HinfI GANTC 5 cut(s) 245, 314, 406, 912, 1368
HpaII CCGG 1 cut(s) 507
HphI GGTGA 5 cut(s) 229, 1294, 1300, 1333, 1341
Hpy166II GTNNAC 7 cut(s) 483, 727, 825, 993, 1225, 1363, 1719
Hpy188I TCNGA 7 cut(s) 94, 223, 319, 411, 598, 977, 1281
Hpy188III TCNNGA 6 cut(s) 158, 403, 496, 736, 770, 968
Hpy8I GTNNAC 7 cut(s) 483, 727, 825, 993, 1225, 1363, 1719
Hpy99I CGWCG 2 cut(s) 286, 1118
HpyAV CCTTC 6 cut(s) 262, 646, 727, 1092, 1429, 1555
HpyCH4III ACNGT 2 cut(s) 1353, 1394
HpyCH4IV ACGT 1 cut(s) 109
HpyF10VI GCNNNNNNNGC 8 cut(s) 123, 435, 684, 1018, 1029, 1115, 1550, 1638
HpyF3I CTNAG 5 cut(s) 361, 410, 842, 1241, 1559
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 8 cut(s) 101, 146, 499, 559, 805, 1171, 1426, 1469
HspAI GCGC 7 cut(s) 581, 676, 678, 764, 1021, 1229, 1379
Kzo9I GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
LmnI GCTCC 5 cut(s) 373, 381, 509, 619, 1047
Lsp1109I GCAGC 4 cut(s) 129, 628, 656, 697
LweI GCATC 3 cut(s) 425, 968, 1366
MaeI CTAG 1 cut(s) 1605
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 4 cut(s) 1066, 1159, 1461, 1522
MalI GATC 9 cut(s) 30, 102, 139, 156, 467, 494, 779, 966, 1085
MboI GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
MboII GAAGA 6 cut(s) 348, 611, 614, 686, 1093, 1184
MfeI CAATTG 1 cut(s) 1187
MhlI GDGCHC 4 cut(s) 294, 378, 1034, 1044
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 4 cut(s) 42, 130, 351, 493
MroXI GAANNNNTTC 1 cut(s) 1367
MseI TTAA 2 cut(s) 425, 1580
MslI CAYNNNNRTG 1 cut(s) 1487
MspCI CTTAAG 1 cut(s) 1579
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 2 cut(s) 275, 909
MunI CAATTG 1 cut(s) 1187
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 2 cut(s) 275, 909
MvnI CGCG 6 cut(s) 281, 581, 678, 771, 1023, 1229
MwoI GCNNNNNNNGC 8 cut(s) 123, 435, 684, 1018, 1029, 1115, 1550, 1638
NdeII GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
NlaIII CATG 8 cut(s) 101, 146, 499, 559, 805, 1171, 1426, 1469
NlaIV GGNNCC 1 cut(s) 1687
NmuCI GTSAC 3 cut(s) 1066, 1159, 1522
NruI TCGCGA 1 cut(s) 771
NspI RCATGY 1 cut(s) 805
PagI TCATGA 1 cut(s) 495
PauI GCGCGC 1 cut(s) 676
PceI AGGCCT 1 cut(s) 1124
PctI GAATGC 1 cut(s) 866
PdmI GAANNNNTTC 1 cut(s) 1367
PfeI GAWTC 4 cut(s) 314, 406, 912, 1368
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 4 cut(s) 119, 643, 646, 712
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Psp124BI GAGCTC 3 cut(s) 294, 378, 1044
Psp1406I AACGTT 1 cut(s) 109
Psp6I CCWGG 2 cut(s) 273, 907
PspGI CCWGG 2 cut(s) 273, 907
PspN4I GGNNCC 1 cut(s) 1687
PspPI GGNCC 1 cut(s) 1183
PstI CTGCAG 2 cut(s) 646, 1060
PteI GCGCGC 1 cut(s) 676
RruI TCGCGA 1 cut(s) 771
RsaI GTAC 2 cut(s) 905, 1598
RsaNI GTAC 2 cut(s) 904, 1597
RseI CAYNNNNRTG 1 cut(s) 1487
SacI GAGCTC 3 cut(s) 294, 378, 1044
SaqAI TTAA 2 cut(s) 425, 1580
SatI GCNGC 4 cut(s) 118, 642, 645, 711
Sau3AI GATC 9 cut(s) 28, 100, 137, 154, 465, 492, 777, 964, 1083
Sau96I GGNCC 1 cut(s) 1183
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 275, 909
SduI GDGCHC 4 cut(s) 294, 378, 1034, 1044
SfaNI GCATC 3 cut(s) 425, 968, 1366
SfcI CTRYAG 2 cut(s) 642, 1056
SinI GGWCC 1 cut(s) 1183
SmiMI CAYNNNNRTG 1 cut(s) 1487
SmlI CTYRAG 2 cut(s) 1412, 1579
SmoI CTYRAG 2 cut(s) 1412, 1579
SseBI AGGCCT 1 cut(s) 1124
SsiI CCGC 5 cut(s) 166, 326, 685, 981, 1493
SspMI CTAG 1 cut(s) 1605
SstI GAGCTC 3 cut(s) 294, 378, 1044
StuI AGGCCT 1 cut(s) 1124
StyD4I CCNGG 2 cut(s) 273, 907
StyI CCWWGG 2 cut(s) 42, 759
TaaI ACNGT 2 cut(s) 1353, 1394
TaiI ACGT 1 cut(s) 112
TfiI GAWTC 4 cut(s) 314, 406, 912, 1368
Tru1I TTAA 2 cut(s) 425, 1580
Tru9I TTAA 2 cut(s) 425, 1580
TscAI CASTG 5 cut(s) 400, 995, 1065, 1158, 1399
TseFI GTSAC 3 cut(s) 1066, 1159, 1522
TseI GCWGC 4 cut(s) 117, 641, 644, 710
Tsp45I GTSAC 3 cut(s) 1066, 1159, 1522
TspDTI ATGAA 7 cut(s) 456, 544, 831, 878, 944, 1360, 1439
TspRI CASTG 5 cut(s) 400, 995, 1065, 1158, 1399
Vha464I CTTAAG 1 cut(s) 1579
VpaK11BI GGWCC 1 cut(s) 1183
XapI RAATTY 2 cut(s) 704, 1129
XceI RCATGY 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 824
XmnI GAANNNNTTC 1 cut(s) 1367
XspI CTAG 1 cut(s) 1605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.