FvH4_5g39090

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005917
Physical Location & Seq
Forward (+)
28847479 .. 28849839
2361 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39090.t1

Sequence Viewer

Length: 1965 bp
ATGGCAAATGCATTCTTCTCTTTCACACCATTTGATTTCGGAATCCAAGGCGGTTTGAGTCCACCCCATCTTGAAGATTATGACAAAGAAGAAGATACAGTCATGTTCAATGTTGGTAAAGGAGACCATTTATTTGGTCTAGATGATCAGTTGGGAGAAATGAACACTCTTTCTTCTGAACATGGTTTCTACCAAGACAACAAGGCCAAGAAAGTAGTAGTAGTATCCCATCTGTCCAAAGAGCAAAACCAACACCATGATATTGACCAGCAGCAGCAACAACAGAAAACCAAACCAAGCTTTTTGATTTTGGATAACTTCAACTTCGAGACTGTTTCTTCTCAACAAGGTTTCTATCATGCCGGGAAGGATGACCAACACCACCATTACCAACAACAGCAACAACAATCCAAGTCAACTTCTTCAATTTTGGAGAATTTCAGCAGCTTTGATTTTAGCTTCCCCTCAGGACAAGCTACACAAACAATTGAGGATGGTGCTCGGTTTACACTTCAGAGCCAGATGGGAGGCTCAAACATTGTCGAAACTAACAAGCAAATGCCGCCTCCTCAATCTTCTTTAGCAACACAGGAGCTACTAAACAGTTACGGAAGCGCCTTTAAGAAGTTGAAGGGAGAAAGGCAAACCAGTAGCAGGGCAGCTAAGGGCATTGAAACCTATCAAAACAGCAACTATGTTGTTAATGGTAAACAGAAACTGTCGACTGAGGAGATCATGAGGGTTGCTGGAGCAAGGTATGTGCAATTCTCTGGTCAGGGGTATGATGACTTTTACATGCCTATGCACCATTTTGGCTATGCGCTTCCAGGTCTTTGCGAAGAAGAAACAAAAGATGTAGAACTTGCTCACTTGCTGTTAGCTATAGCAGAGAAAGTAGGCTACCAACAATTCGAAAGAGCTAGCAGATTGCTTGTGCAGTGTGAATGGTCTGCTTCATTGAAAGGCACTACTGTTCAGAGAGTTGTGTGCTACTTTGCTGAGGCGCTACGCGAGAGGATCAAAAAAGAAACAGGAATGTATGCATCTAAGGGAAATCAAGAAATGTCTCATTATGTACAGGGTTTGGGGACAAACCTTGCATACATCGTATGCCACCAAGAAGTCCCTTTCCATCTAGTACTGCAGTTCGCGGCAATCCAAGCCATGATAGAAACTGTTGCAAATGAGAGAAGGATCCATTTGATTGATCTTGAAATTAGGAGTGGTGTGCAATGGACAGGGTTTATGGAAGCACTAGCAGAACGAGAGGAGTGTCCGGTTGAGCTTCTAACCATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTAGCAAGTGTGGCAAAGTCCTTGAACCTACCCTTTGAGTACAAGGAAGTTATTGTATCAGACATGCAAGACATTAAGGATCATCTTTTCGATGAAATTGAAGATGAAGAAGCTGTGGTTGTGTACGCGCCATTAGTACTGAGGACAATGATAATGAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATATCAACCCTTGTTTGATGGTGGTCATGGAAGTAGAAGCAAACCATAACTCACCGTCATTTGTGAACCGTTTCATTGATGCATTGTTTTACTATAGTGCATTTTTTGACTGCCTTGAAAATTGTATGAAACATAACAAGTACAGGGTTTTCATTGAAGACTTGTTACGCAAGGGAATCCAGAACATAGTGGCGGCAGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGAACTTTCTTTGCAAGGTTTAGAATGGTAGAAATGAGCTTTAGTAACTCATCTTTGTATCAAGCAAGTCTGGTGGCTAAGAAGTATTGTTGCACAACACTTGATAGGAATGGAAAATGTCTAACTGTTGGATGGAAGGGAACCCCAATTCATTCGCTTTCAGCTTGGAAGTTCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

655

Amino Acids

74.55

Weight (kDa)

5.99

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 289 - 652 1.5e-78 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 722
AccII CGCG 3 cut(s) 1011, 1151, 1475
AciI CCGC 4 cut(s) 51, 563, 1151, 1736
AclWI GGATC 4 cut(s) 1025, 1189, 1202, 1434
AcsI RAATTY 1 cut(s) 436
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 6 cut(s) 1077, 1140, 1388, 1472, 1485, 1685
AfiI CCNNNNNNNGG 1 cut(s) 654
AjnI CCWGG 1 cut(s) 826
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 4 cut(s) 117, 323, 1071, 1328
AlwI GGATC 4 cut(s) 1025, 1189, 1202, 1434
AlwNI CAGNNNCTG 1 cut(s) 718
AoxI GGCC 2 cut(s) 204, 1505
ApeKI GCWGC 4 cut(s) 271, 274, 444, 659
ApoI RAATTY 1 cut(s) 436
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
Asp700I GAANNNNTTC 1 cut(s) 1613
AspA2I CCTAGG 1 cut(s) 1508
AspLEI GCGC 4 cut(s) 617, 823, 1006, 1477
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 2 cut(s) 1546, 1587
AsuII TTCGAA 1 cut(s) 912
AsuNHI GCTAGC 1 cut(s) 920
AvrII CCTAGG 1 cut(s) 1508
AxyI CCTNAGG 1 cut(s) 466
BamHI GGATCC 1 cut(s) 1194
BarI GAAGNNNNNNTAC 2 cut(s) 1692, 1724
BbsI GAAGAC 1 cut(s) 1707
Bbv12I GWGCWC 1 cut(s) 502
BbvCI CCTCAGC 1 cut(s) 999
BbvI GCAGC 4 cut(s) 283, 286, 456, 671
BccI CCATC 8 cut(s) 75, 237, 488, 517, 1140, 1555, 1768, 1911
BciT130I CCWGG 1 cut(s) 828
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 4 cut(s) 117, 323, 1071, 1328
BfaI CTAG 5 cut(s) 140, 921, 1136, 1256, 1509
BfmI CTRYAG 3 cut(s) 882, 1142, 1636
BfoI RGCGCY 2 cut(s) 618, 1007
BfuI GTATCC 1 cut(s) 235
BisI GCNGC 7 cut(s) 272, 275, 445, 563, 660, 1152, 1737
BlnI CCTAGG 1 cut(s) 1508
BlsI GCNGC 7 cut(s) 273, 276, 446, 564, 661, 1153, 1738
BmcAI AGTACT 2 cut(s) 1140, 1485
Bme1390I CCNGG 2 cut(s) 364, 828
BmiI GGNNCC 2 cut(s) 1196, 1927
BmrFI CCNGG 2 cut(s) 364, 828
BmsI GCATC 2 cut(s) 1052, 1612
BmtI GCTAGC 1 cut(s) 924
BpiI GAAGAC 1 cut(s) 1707
BpmI CTGGAG 1 cut(s) 768
Bpu10I CCTNAGC 2 cut(s) 663, 999
Bpu14I TTCGAA 1 cut(s) 912
BpuMI CCSGG 1 cut(s) 364
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 46, 1508
BsaWI WCCGGW 1 cut(s) 1276
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse1I ACTGG 1 cut(s) 648
Bse21I CCTNAGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 1238
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 2 cut(s) 46, 1508
BseGI GGATG 4 cut(s) 376, 499, 1783, 1922
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMI GCAATG 1 cut(s) 1238
BseMII CTCAG 4 cut(s) 480, 717, 990, 1478
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 3 cut(s) 558, 743, 1283
BseXI GCAGC 4 cut(s) 283, 286, 456, 671
BsgI GTGCAG 1 cut(s) 956
Bsh1236I CGCG 3 cut(s) 1011, 1151, 1475
BshFI GGCC 2 cut(s) 206, 1507
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 2 cut(s) 363, 1277
BslFI GGGAC 2 cut(s) 1102, 1109
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 4 cut(s) 117, 323, 1071, 1328
BsmFI GGGAC 2 cut(s) 1102, 1109
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 2 cut(s) 206, 1507
Bso31I GGTCTC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
BspACI CCGC 4 cut(s) 51, 563, 1151, 1736
BspANI GGCC 2 cut(s) 206, 1507
BspCNI CTCAG 4 cut(s) 479, 718, 991, 1479
BspFNI CGCG 3 cut(s) 1011, 1151, 1475
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 1196, 1927
BspMAI CTGCAG 1 cut(s) 1146
BspOI GCTAGC 1 cut(s) 924
BspPI GGATC 4 cut(s) 1025, 1189, 1202, 1434
BspT104I TTCGAA 1 cut(s) 912
BspTNI GGTCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 1238
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 2 cut(s) 46, 1508
BssMI GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
BssT1I CCWWGG 2 cut(s) 46, 1508
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 9 cut(s) 100, 334, 605, 720, 973, 1177, 1599, 1613, 1912
BstAUI TGTACA 1 cut(s) 1075
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 1 cut(s) 922
BstDEI CTNAG 7 cut(s) 466, 663, 726, 999, 1047, 1487, 1863
BstF5I GGATG 4 cut(s) 376, 499, 1783, 1922
BstFNI CGCG 3 cut(s) 1011, 1151, 1475
BstH2I RGCGCY 2 cut(s) 618, 1007
BstHHI GCGC 4 cut(s) 617, 823, 1006, 1477
BstKTI GATC 6 cut(s) 148, 735, 1020, 1197, 1210, 1429
BstMAI GTCTC 4 cut(s) 117, 323, 1071, 1328
BstMBI GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
BstMWI GCNNNNNNNGC 3 cut(s) 562, 1160, 1358
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 2 cut(s) 799, 1414
BstSCI CCNGG 2 cut(s) 362, 826
BstSFI CTRYAG 3 cut(s) 882, 1142, 1636
BstUI CGCG 3 cut(s) 1011, 1151, 1475
BstV1I GCAGC 4 cut(s) 283, 286, 456, 671
BstV2I GAAGAC 1 cut(s) 1707
BstX2I RGATCY 1 cut(s) 1194
BstXI CCANNNNNNTGG 1 cut(s) 134
BstYI RGATCY 1 cut(s) 1194
Bsu36I CCTNAGG 1 cut(s) 466
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 206, 1507
BtsCI GGATG 4 cut(s) 376, 499, 1783, 1922
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 1 cut(s) 944
Cac8I GCNNGC 1 cut(s) 922
CaiI CAGNNNCTG 1 cut(s) 718
CciI TCATGA 1 cut(s) 735
CfoI GCGC 4 cut(s) 617, 823, 1006, 1477
Csp6I GTAC 6 cut(s) 1076, 1139, 1387, 1471, 1484, 1684
CspCI CAANNNNNGTGG 2 cut(s) 1839, 1874
CviAII CATG 9 cut(s) 103, 182, 257, 359, 736, 796, 1165, 1411, 1570
CviQI GTAC 6 cut(s) 1076, 1139, 1387, 1471, 1484, 1684
DdeI CTNAG 7 cut(s) 466, 663, 726, 999, 1047, 1487, 1863
DpnI GATC 6 cut(s) 147, 734, 1019, 1196, 1209, 1428
DpnII GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
Eco130I CCWWGG 2 cut(s) 46, 1508
Eco147I AGGCCT 1 cut(s) 1507
Eco31I GGTCTC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 46, 1508
EcoT22I ATGCAT 3 cut(s) 13, 1045, 1627
ErhI CCWWGG 2 cut(s) 46, 1508
FaeI CATG 9 cut(s) 106, 185, 260, 362, 739, 799, 1168, 1414, 1573
FaqI GGGAC 2 cut(s) 1102, 1109
FatI CATG 9 cut(s) 102, 181, 256, 358, 735, 795, 1164, 1410, 1569
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 722
Fnu4HI GCNGC 7 cut(s) 272, 275, 445, 563, 660, 1152, 1737
FokI GGATG 4 cut(s) 383, 506, 1790, 1929
Fsp4HI GCNGC 7 cut(s) 272, 275, 445, 563, 660, 1152, 1737
FspBI CTAG 5 cut(s) 140, 921, 1136, 1256, 1509
GlaI GCGC 4 cut(s) 616, 822, 1005, 1476
GluI GCNGC 7 cut(s) 272, 275, 445, 563, 660, 1152, 1737
GsuI CTGGAG 1 cut(s) 768
HaeII RGCGCY 2 cut(s) 618, 1007
HaeIII GGCC 2 cut(s) 206, 1507
HapII CCGG 2 cut(s) 363, 1277
HhaI GCGC 4 cut(s) 617, 823, 1006, 1477
Hin1II CATG 9 cut(s) 106, 185, 260, 362, 739, 799, 1168, 1414, 1573
Hin6I GCGC 4 cut(s) 615, 821, 1004, 1475
HinP1I GCGC 4 cut(s) 615, 821, 1004, 1475
HincII GTYRAC 2 cut(s) 417, 723
HindII GTYRAC 2 cut(s) 417, 723
HindIII AAGCTT 1 cut(s) 298
HinfI GANTC 3 cut(s) 42, 58, 1719
HpaII CCGG 2 cut(s) 363, 1277
HphI GGTGA 2 cut(s) 1546, 1587
Hpy166II GTNNAC 7 cut(s) 62, 417, 507, 710, 723, 1471, 1609
Hpy188I TCNGA 5 cut(s) 41, 178, 516, 978, 1408
Hpy188III TCNNGA 8 cut(s) 71, 140, 328, 468, 736, 1058, 1211, 1723
Hpy8I GTNNAC 7 cut(s) 62, 417, 507, 710, 723, 1471, 1609
HpyAV CCTTC 5 cut(s) 361, 625, 1185, 1736, 1915
HpyCH4III ACNGT 9 cut(s) 100, 334, 605, 720, 973, 1177, 1599, 1613, 1912
HpyF10VI GCNNNNNNNGC 3 cut(s) 562, 1160, 1358
HpyF3I CTNAG 7 cut(s) 466, 663, 726, 999, 1047, 1487, 1863
Hsp92II CATG 9 cut(s) 106, 185, 260, 362, 739, 799, 1168, 1414, 1573
HspAI GCGC 4 cut(s) 615, 821, 1004, 1475
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
LmnI GCTCC 2 cut(s) 592, 749
Lsp1109I GCAGC 4 cut(s) 283, 286, 456, 671
LweI GCATC 2 cut(s) 1052, 1612
MaeI CTAG 5 cut(s) 140, 921, 1136, 1256, 1509
MaeIII GTNAC 3 cut(s) 605, 1707, 1829
MalI GATC 6 cut(s) 147, 734, 1019, 1196, 1209, 1428
MboI GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 1194
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 9 cut(s) 426, 436, 486, 764, 908, 1215, 1443, 1663, 1932
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 1894
Mph1103I ATGCAT 3 cut(s) 13, 1045, 1627
MroXI GAANNNNTTC 1 cut(s) 1613
MseI TTAA 3 cut(s) 621, 702, 1422
MslI CAYNNNNRTG 1 cut(s) 800
MspI CCGG 2 cut(s) 363, 1277
MspR9I CCNGG 2 cut(s) 364, 828
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 3 cut(s) 1011, 1151, 1475
MwoI GCNNNNNNNGC 3 cut(s) 562, 1160, 1358
NciI CCSGG 1 cut(s) 364
NdeII GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
NheI GCTAGC 1 cut(s) 920
NlaIII CATG 9 cut(s) 106, 185, 260, 362, 739, 799, 1168, 1414, 1573
NlaIV GGNNCC 2 cut(s) 1196, 1927
NsiI ATGCAT 3 cut(s) 13, 1045, 1627
NspI RCATGY 2 cut(s) 799, 1414
NspV TTCGAA 1 cut(s) 912
PagI TCATGA 1 cut(s) 735
PceI AGGCCT 1 cut(s) 1507
PctI GAATGC 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 1613
PfeI GAWTC 2 cut(s) 42, 1719
PkrI GCNGC 7 cut(s) 273, 276, 446, 564, 661, 1153, 1738
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 2 cut(s) 1196, 1927
PstI CTGCAG 1 cut(s) 1146
PstNI CAGNNNCTG 1 cut(s) 718
PsuI RGATCY 1 cut(s) 1194
RsaI GTAC 6 cut(s) 1077, 1140, 1388, 1472, 1485, 1685
RsaNI GTAC 6 cut(s) 1076, 1139, 1387, 1471, 1484, 1684
RseI CAYNNNNRTG 1 cut(s) 800
SalI GTCGAC 1 cut(s) 721
SaqAI TTAA 3 cut(s) 621, 702, 1422
SatI GCNGC 7 cut(s) 272, 275, 445, 563, 660, 1152, 1737
Sau3AI GATC 6 cut(s) 145, 732, 1017, 1194, 1207, 1426
ScaI AGTACT 2 cut(s) 1140, 1485
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 364, 828
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 2 cut(s) 1052, 1612
SfcI CTRYAG 3 cut(s) 882, 1142, 1636
SfuI TTCGAA 1 cut(s) 912
SmiMI CAYNNNNRTG 1 cut(s) 800
Sse9I AATT 9 cut(s) 426, 436, 486, 764, 908, 1215, 1443, 1663, 1932
SseBI AGGCCT 1 cut(s) 1507
SsiI CCGC 4 cut(s) 51, 563, 1151, 1736
SspMI CTAG 5 cut(s) 140, 921, 1136, 1256, 1509
StuI AGGCCT 1 cut(s) 1507
StyD4I CCNGG 2 cut(s) 362, 826
StyI CCWWGG 2 cut(s) 46, 1508
TaaI ACNGT 9 cut(s) 100, 334, 605, 720, 973, 1177, 1599, 1613, 1912
TaqI TCGA 6 cut(s) 327, 543, 722, 912, 1437, 1762
TasI AATT 9 cut(s) 426, 436, 486, 764, 908, 1215, 1443, 1663, 1932
TatI WGTACW 5 cut(s) 1075, 1138, 1386, 1483, 1683
TauI GCSGC 3 cut(s) 565, 1154, 1739
TfiI GAWTC 2 cut(s) 42, 1719
Tru1I TTAA 3 cut(s) 621, 702, 1422
Tru9I TTAA 3 cut(s) 621, 702, 1422
TscAI CASTG 1 cut(s) 944
TseI GCWGC 4 cut(s) 271, 274, 444, 659
TspDTI ATGAA 8 cut(s) 176, 945, 1455, 1467, 1606, 1684, 1685, 1925
TspGWI ACGGA 2 cut(s) 624, 1949
TspRI CASTG 1 cut(s) 944
XapI RAATTY 1 cut(s) 436
XbaI TCTAGA 1 cut(s) 139
XceI RCATGY 2 cut(s) 799, 1414
XcmI CCANNNNNNNNNTGG 1 cut(s) 1300
XmaJI CCTAGG 1 cut(s) 1508
XmiI GTMKAC 1 cut(s) 722
XmnI GAANNNNTTC 1 cut(s) 1613
XspI CTAG 5 cut(s) 140, 921, 1136, 1256, 1509
ZrmI AGTACT 2 cut(s) 1140, 1485
Zsp2I ATGCAT 3 cut(s) 13, 1045, 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.