Rh7BG468200

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005917
Physical Location & Seq
Forward (+)
55374750 .. 55376826
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG468200.1

Sequence Viewer

Length: 1980 bp
ATGGCAAATGCATTATTCTCGTTCACACCATTTGATTTCGATATCCGAGGCGGTTTGAGTCCCCCCCAGCTTGAAGATTATGACAAAGAAGAAACAGTAATGTTCAATGTTGGCAAGGGAGAGCATTTGTTTGGACTAGAAGATCAGTTGGGAGGAATGAATACTCCTCCTTCTGAACATGGTTTCTACCAAGACAACAACGCCAAGACAGGAGTAGTAGTATGCCATTTGTCCAAAGATCAAAACCAACACCGTGATCTTGAACACCAACAGCAGCAACAGCAATCCAAGACAGACTTTTTGATTCTGGATGACTTCAACTTCGAGACTGTTTCTTCTCAACATGGTTTTTATCATGCCAATAAGGTGGGGAAGGATCACCAACACCACCATTACCAACAACAGCAACAACAATCCAAGTCAAGTTTTTCAATTTTGGAGAATTTCAATAACTTTGATTTTTACTTCCCCTCGGGACAAGTTACAGAACCAGTTGAGGATGGTGCTCGGTTTACACACAGCAAGATTAGAGGCTCAGACATTTTCGAAACGAACAAGCAATTGCCTCCTCCTCAATCTTCTCTAGGAACACTGGAGCTCCTTAACAGTTACGGAAGCGCCTTCAAGAAGTTGAAGGGAGAAAGACAAAGCAGTAGCAGGGCAGTTAAGGGTAATGAAATTGATCAAAACAGCAGGTACGTTGTTAATGGTAATCAGAAACTGTCCACCGAAGAGGTCATGAGGGTTGCTGGAGCAAGGTATGTGCAATTCTCTAGTCAGGGGTATGAAAATTTGTACATGTCTATGCACCCTTTTGGCTATGCGCTTTCAGGTCTCTGCGAAGAAGAAACGAAAGATGTAGAACTTGTTCACTTACTGTTAGCTGCAGCAGAGAAAGTAGGGTACCAACAATTTGAAAGAGCAAGCAGATTGCTTCTGCAGTGTGAATGGATTCCATCCTTCAAAGGTACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCAGAGGCACTTCGTGAGAGGATCGAAAAAGAAACAGGACAGTATACATCTAAGGGAAATGAAGAATTGTCTCAGTATGCACATGGTTTGGGGACAAACCTTGCATACATCGCATGCCACCAAGAAGTCCCTTTCCATCCAGTACTGCAGTTCGCTGCAATCCAAGCCATGATCGAAAATGTTGCAAATGAGAGTAGGATCCATTTGATTGATCTGGAAATCAGGAGTGGTGTGCAATGGACAGGGTGGATGGAAGCAATTGCAGAACGAGAGGAATGCCCGGTTAAGCTTCTTACTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGGCAAGTGTTGCAATGTCCTTGAACCTACCCTTTAAATTTAAGGAAGTTATTGTATCAGACATGCAAGACATTAATAACCAGTTATTCGAAGACATTGAAGATGATGAAGCTGTGATTGTGTACGCGCCATTAATACTGAGGACAATGATAGCAAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATATCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCCCCATCATTTGTGCACCGTTTCATTGATACATTGTTTTTTTATAGTGCATTTTTCGACTGCCTTGAAACCTGTATGAAACAGGAGAAGTACAGGGTGATCATGGAAGGCTGGTTACGTGAGGGAATCCGGAACATAGTGGCGGCGGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCAAGGTTTAGCATGGTGGAAATGAGCTTTAGTAACTCATCATTTTATCTAGCTAGTCTAGTGGCTAAAAAGTTCTGTTGCACCACACTTGATAGGAATGGAAAATGTCTCACTGTTGGGTGGAAGGGAACCCCAATTCATTCTCTTTCCGCTTGGAAGTTCCGTCTTGAAAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

659

Amino Acids

75.21

Weight (kDa)

5.82

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 289 - 653 5.9e-75 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 684
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 903
AccI GTMKAC 1 cut(s) 1043
AccII CGCG 1 cut(s) 1478
AccIII TCCGGA 1 cut(s) 1725
AciI CCGC 4 cut(s) 51, 1739, 1742, 1950
AclWI GGATC 4 cut(s) 384, 1028, 1192, 1205
AcsI RAATTY 3 cut(s) 442, 790, 1388
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 7 cut(s) 698, 797, 905, 970, 1143, 1475, 1688
AflIII ACRYGT 1 cut(s) 798
AluBI AGCT 8 cut(s) 70, 598, 884, 1288, 1463, 1586, 1827, 1853
AluI AGCT 8 cut(s) 70, 598, 884, 1288, 1463, 1586, 1827, 1853
Alw21I GWGCWC 3 cut(s) 508, 600, 1614
Alw26I GTCTC 5 cut(s) 320, 839, 1074, 1331, 1913
Alw44I GTGCAC 1 cut(s) 1610
AlwI GGATC 4 cut(s) 384, 1028, 1192, 1205
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 472
Aor13HI TCCGGA 1 cut(s) 1725
AoxI GGCC 2 cut(s) 1508, 1791
ApaLI GTGCAC 1 cut(s) 1610
ApeKI GCWGC 4 cut(s) 274, 884, 887, 1154
ApoI RAATTY 3 cut(s) 442, 790, 1388
ArsI GACNNNNNNTTYG 4 cut(s) 283, 305, 315, 337
AseI ATTAAT 2 cut(s) 1425, 1484
Asp700I GAANNNNTTC 2 cut(s) 867, 951
Asp718I GGTACC 1 cut(s) 903
AspA2I CCTAGG 1 cut(s) 1511
AspLEI GCGC 3 cut(s) 620, 826, 1480
AspS9I GGNCC 1 cut(s) 1791
AsuC2I CCSGG 1 cut(s) 1280
AsuHPI GGTGA 3 cut(s) 371, 1549, 1705
AsuII TTCGAA 2 cut(s) 546, 1440
AvaI CYCGRG 1 cut(s) 472
AvrII CCTAGG 1 cut(s) 1511
BaeGI GKGCMC 1 cut(s) 1614
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BamHI GGATCC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 903
BanII GRGCYC 1 cut(s) 600
BarI GAAGNNNNNNTAC 2 cut(s) 1695, 1727
BbsI GAAGAC 1 cut(s) 1449
Bbv12I GWGCWC 3 cut(s) 508, 600, 1614
BbvI GCAGC 4 cut(s) 286, 871, 899, 1141
BccI CCATC 7 cut(s) 494, 964, 1143, 1243, 1558, 1609, 1771
BcgI CGANNNNNNTGC 5 cut(s) 34, 1163, 1197, 1257, 1291
BclI TGATCA 2 cut(s) 682, 1695
BcnI CCSGG 1 cut(s) 1280
BcoDI GTCTC 5 cut(s) 320, 839, 1074, 1331, 1913
BfaI CTAG 7 cut(s) 137, 584, 774, 1512, 1850, 1854, 1859
BfmI CTRYAG 3 cut(s) 885, 938, 1145
BfoI RGCGCY 1 cut(s) 621
BfuAI ACCTGC 1 cut(s) 684
BisI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
BlnI CCTAGG 1 cut(s) 1511
BlsI GCNGC 5 cut(s) 276, 886, 889, 1156, 1741
BmcAI AGTACT 1 cut(s) 1143
Bme1390I CCNGG 1 cut(s) 1280
BmeT110I CYCGRG 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 1791
BmiI GGNNCC 3 cut(s) 905, 1199, 1930
BmrFI CCNGG 1 cut(s) 1280
BpiI GAAGAC 1 cut(s) 1449
BpmI CTGGAG 2 cut(s) 614, 771
Bpu14I TTCGAA 2 cut(s) 546, 1440
BpuMI CCSGG 1 cut(s) 1280
BsaAI YACGTR 1 cut(s) 1715
BsaI GGTCTC 1 cut(s) 839
BsaJI CCNNGG 3 cut(s) 46, 471, 1511
BsaWI WCCGGW 1 cut(s) 1725
BsaXI ACNNNNNCTCC 6 cut(s) 204, 234, 582, 612, 1580, 1610
Bse1I ACTGG 4 cut(s) 491, 597, 1139, 1432
Bse3DI GCAATG 2 cut(s) 1241, 1371
BseAI TCCGGA 1 cut(s) 1725
BseDI CCNNGG 3 cut(s) 46, 471, 1511
BseGI GGATG 6 cut(s) 316, 505, 956, 1135, 1254, 1786
BseMI GCAATG 2 cut(s) 1241, 1371
BseMII CTCAG 3 cut(s) 549, 1085, 1481
BseNI ACTGG 4 cut(s) 491, 597, 1139, 1432
BseRI GAGGAG 3 cut(s) 156, 558, 561
BseSI GKGCMC 1 cut(s) 1614
BseXI GCAGC 4 cut(s) 286, 871, 899, 1141
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 1478
BshFI GGCC 2 cut(s) 1510, 1793
BshNI GGYRCC 1 cut(s) 903
BsiHKAI GWGCWC 3 cut(s) 508, 600, 1614
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 2 cut(s) 1280, 1726
BslFI GGGAC 4 cut(s) 45, 489, 1105, 1112
BsmAI GTCTC 5 cut(s) 320, 839, 1074, 1331, 1913
BsmFI GGGAC 4 cut(s) 45, 489, 1105, 1112
BsmI GAATGC 1 cut(s) 1280
BsnI GGCC 2 cut(s) 1510, 1793
Bso31I GGTCTC 1 cut(s) 839
BsoBI CYCGRG 1 cut(s) 472
Bsp119I TTCGAA 2 cut(s) 546, 1440
Bsp1286I GDGCHC 3 cut(s) 508, 600, 1614
Bsp13I TCCGGA 1 cut(s) 1725
Bsp1407I TGTACA 1 cut(s) 795
BspACI CCGC 4 cut(s) 51, 1739, 1742, 1950
BspANI GGCC 2 cut(s) 1510, 1793
BspCNI CTCAG 3 cut(s) 548, 1084, 1482
BspEI TCCGGA 1 cut(s) 1725
BspFNI CGCG 1 cut(s) 1478
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 3 cut(s) 905, 1199, 1930
BspMAI CTGCAG 3 cut(s) 889, 942, 1149
BspMI ACCTGC 1 cut(s) 684
BspPI GGATC 4 cut(s) 384, 1028, 1192, 1205
BspT104I TTCGAA 2 cut(s) 546, 1440
BspT107I GGYRCC 1 cut(s) 903
BspTNI GGTCTC 1 cut(s) 839
BsrDI GCAATG 2 cut(s) 1241, 1371
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 4 cut(s) 491, 597, 1139, 1432
BssECI CCNNGG 3 cut(s) 46, 471, 1511
BssNAI GTATAC 1 cut(s) 1044
BssT1I CCWWGG 1 cut(s) 1511
Bst1107I GTATAC 1 cut(s) 1044
Bst4CI ACNGT 9 cut(s) 97, 254, 331, 608, 723, 879, 1041, 1616, 1915
Bst6I CTCTTC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 1361
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 1 cut(s) 1715
BstBI TTCGAA 2 cut(s) 546, 1440
BstC8I GCNNGC 2 cut(s) 925, 1114
BstDEI CTNAG 4 cut(s) 535, 1050, 1071, 1490
BstF5I GGATG 6 cut(s) 316, 505, 956, 1135, 1254, 1786
BstFNI CGCG 1 cut(s) 1478
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 620, 826, 1480
BstMAI GTCTC 5 cut(s) 320, 839, 1074, 1331, 1913
BstMWI GCNNNNNNNGC 5 cut(s) 280, 1109, 1163, 1361, 1799
BstNSI RCATGY 3 cut(s) 802, 1116, 1417
BstSCI CCNGG 1 cut(s) 1278
BstSFI CTRYAG 3 cut(s) 885, 938, 1145
BstSLI GKGCMC 1 cut(s) 1614
BstUI CGCG 1 cut(s) 1478
BstV1I GCAGC 4 cut(s) 286, 871, 899, 1141
BstV2I GAAGAC 1 cut(s) 1449
BstX2I RGATCY 1 cut(s) 1197
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 1197
BstZ17I GTATAC 1 cut(s) 1044
BsuRI GGCC 2 cut(s) 1510, 1793
BtgZI GCGATG 1 cut(s) 1093
BtsCI GGATG 6 cut(s) 316, 505, 956, 1135, 1254, 1786
BtsI GCAGTG 1 cut(s) 947
BtsIMutI CAGTG 3 cut(s) 590, 947, 1911
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 2 cut(s) 925, 1114
CaiI CAGNNNCTG 1 cut(s) 721
CciI TCATGA 1 cut(s) 738
CfoI GCGC 3 cut(s) 620, 826, 1480
Cfr13I GGNCC 1 cut(s) 1791
Csp6I GTAC 7 cut(s) 697, 796, 904, 969, 1142, 1474, 1687
CviQI GTAC 7 cut(s) 697, 796, 904, 969, 1142, 1474, 1687
DdeI CTNAG 4 cut(s) 535, 1050, 1071, 1490
DraI TTTAAA 1 cut(s) 1387
DraIII CACNNNGTG 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
EciI GGCGGA 1 cut(s) 1757
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 1511
Eco147I AGGCCT 1 cut(s) 1510
Eco24I GRGCYC 1 cut(s) 600
Eco31I GGTCTC 1 cut(s) 839
Eco32I GATATC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 1791
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 1511
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 1511
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 4 cut(s) 45, 489, 1105, 1112
FbaI TGATCA 2 cut(s) 682, 1695
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
FokI GGATG 6 cut(s) 323, 512, 943, 1122, 1261, 1793
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
FspBI CTAG 7 cut(s) 137, 584, 774, 1512, 1850, 1854, 1859
GlaI GCGC 3 cut(s) 619, 825, 1479
GluI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 614, 771
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 2 cut(s) 1510, 1793
HapII CCGG 2 cut(s) 1280, 1726
HhaI GCGC 3 cut(s) 620, 826, 1480
Hin6I GCGC 3 cut(s) 618, 824, 1478
HinP1I GCGC 3 cut(s) 618, 824, 1478
HindIII AAGCTT 1 cut(s) 1286
HinfI GANTC 4 cut(s) 58, 304, 952, 1722
HpaII CCGG 2 cut(s) 1280, 1726
HphI GGTGA 3 cut(s) 371, 1549, 1705
Hpy166II GTNNAC 7 cut(s) 24, 513, 726, 871, 1044, 1474, 1612
Hpy188I TCNGA 6 cut(s) 47, 175, 538, 717, 981, 1411
Hpy8I GTNNAC 7 cut(s) 24, 513, 726, 871, 1044, 1474, 1612
HpyCH4III ACNGT 9 cut(s) 97, 254, 331, 608, 723, 879, 1041, 1616, 1915
HpyCH4IV ACGT 2 cut(s) 699, 1714
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 1109, 1163, 1361, 1799
HpyF3I CTNAG 4 cut(s) 535, 1050, 1071, 1490
HpySE526I ACGT 2 cut(s) 699, 1714
HspAI GCGC 3 cut(s) 618, 824, 1478
Kpn2I TCCGGA 1 cut(s) 1725
KpnI GGTACC 1 cut(s) 907
Ksp22I TGATCA 2 cut(s) 682, 1695
LmnI GCTCC 3 cut(s) 595, 603, 752
Lsp1109I GCAGC 4 cut(s) 286, 871, 899, 1141
MaeI CTAG 7 cut(s) 137, 584, 774, 1512, 1850, 1854, 1859
MaeII ACGT 2 cut(s) 699, 1714
MaeIII GTNAC 4 cut(s) 481, 608, 1710, 1832
MfeI CAATTG 2 cut(s) 560, 1257
MflI RGATCY 1 cut(s) 1197
MhlI GDGCHC 3 cut(s) 508, 600, 1614
MlyI GAGTC 1 cut(s) 67
Mph1103I ATGCAT 1 cut(s) 13
MroI TCCGGA 1 cut(s) 1725
MroXI GAANNNNTTC 2 cut(s) 867, 951
MseI TTAA 8 cut(s) 603, 666, 705, 1284, 1386, 1392, 1425, 1484
MslI CAYNNNNRTG 2 cut(s) 803, 1577
MspI CCGG 2 cut(s) 1280, 1726
MspR9I CCNGG 1 cut(s) 1280
MunI CAATTG 2 cut(s) 560, 1257
Mva1269I GAATGC 1 cut(s) 1280
MvnI CGCG 1 cut(s) 1478
MwoI GCNNNNNNNGC 5 cut(s) 280, 1109, 1163, 1361, 1799
NciI CCSGG 1 cut(s) 1280
NlaIV GGNNCC 3 cut(s) 905, 1199, 1930
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 802, 1116, 1417
NspV TTCGAA 2 cut(s) 546, 1440
PaeI GCATGC 1 cut(s) 1116
PagI TCATGA 1 cut(s) 738
PceI AGGCCT 1 cut(s) 1510
PciI ACATGT 1 cut(s) 798
PctI GAATGC 1 cut(s) 1280
PdmI GAANNNNTTC 2 cut(s) 867, 951
PfeI GAWTC 3 cut(s) 304, 952, 1722
PkrI GCNGC 5 cut(s) 276, 886, 889, 1156, 1741
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Ppu21I YACGTR 1 cut(s) 1715
PscI ACATGT 1 cut(s) 798
PshBI ATTAAT 2 cut(s) 1425, 1484
Psp124BI GAGCTC 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 905, 1199, 1930
PspPI GGNCC 1 cut(s) 1791
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 3 cut(s) 889, 942, 1149
PstNI CAGNNNCTG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 1197
RsaI GTAC 7 cut(s) 698, 797, 905, 970, 1143, 1475, 1688
RsaNI GTAC 7 cut(s) 697, 796, 904, 969, 1142, 1474, 1687
RseI CAYNNNNRTG 2 cut(s) 803, 1577
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 8 cut(s) 603, 666, 705, 1284, 1386, 1392, 1425, 1484
SatI GCNGC 5 cut(s) 275, 885, 888, 1155, 1740
Sau96I GGNCC 1 cut(s) 1791
ScaI AGTACT 1 cut(s) 1143
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 1280
SduI GDGCHC 3 cut(s) 508, 600, 1614
SfcI CTRYAG 3 cut(s) 885, 938, 1145
SfuI TTCGAA 2 cut(s) 546, 1440
SmiMI CAYNNNNRTG 2 cut(s) 803, 1577
SphI GCATGC 1 cut(s) 1116
SseBI AGGCCT 1 cut(s) 1510
SsiI CCGC 4 cut(s) 51, 1739, 1742, 1950
SspMI CTAG 7 cut(s) 137, 584, 774, 1512, 1850, 1854, 1859
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 1 cut(s) 1510
StyD4I CCNGG 1 cut(s) 1278
StyI CCWWGG 1 cut(s) 1511
TaaI ACNGT 9 cut(s) 97, 254, 331, 608, 723, 879, 1041, 1616, 1915
TaiI ACGT 2 cut(s) 702, 1717
TaqI TCGA 8 cut(s) 39, 324, 546, 1023, 1173, 1440, 1653, 1765
TatI WGTACW 3 cut(s) 795, 1141, 1686
TauI GCSGC 1 cut(s) 1742
TfiI GAWTC 3 cut(s) 304, 952, 1722
Tru1I TTAA 8 cut(s) 603, 666, 705, 1284, 1386, 1392, 1425, 1484
Tru9I TTAA 8 cut(s) 603, 666, 705, 1284, 1386, 1392, 1425, 1484
TscAI CASTG 3 cut(s) 597, 947, 1918
TseI GCWGC 4 cut(s) 274, 884, 887, 1154
TspDTI ATGAA 8 cut(s) 173, 690, 801, 1074, 1473, 1609, 1688, 1928
TspGWI ACGGA 2 cut(s) 627, 1952
TspRI CASTG 3 cut(s) 597, 947, 1918
VneI GTGCAC 1 cut(s) 1610
VspI ATTAAT 2 cut(s) 1425, 1484
XapI RAATTY 3 cut(s) 442, 790, 1388
XceI RCATGY 3 cut(s) 802, 1116, 1417
XmaJI CCTAGG 1 cut(s) 1511
XmiI GTMKAC 1 cut(s) 1043
XmnI GAANNNNTTC 2 cut(s) 867, 951
XspI CTAG 7 cut(s) 137, 584, 774, 1512, 1850, 1854, 1859
ZrmI AGTACT 1 cut(s) 1143
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.