Rh7DG481300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005917
Physical Location & Seq
Forward (+)
68651104 .. 68673595
22492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG481300.1

Sequence Viewer

Length: 1911 bp
ATGGCAAATGCATTCTTCTCGTACACACCATTTGATTTCGAAATCCAAGGCGGTTTGAGTCCACCCCAGCTTGAAGATTATGACAAAGAAGACACAGTAATGTTCAATGTTCGCAAGGGAGATCATTTGTTTGGTCTAGAAGATCAGTTGGGAGAAATGAATACTCCTTCTGAACATGGTTTCTACCTAGACAACAACAAGGCCAAGAAAGGAGTAGTAGTATCCCATTTGTCCAAAGATCAAAACCAACACCATGATCTTGACCACCAACAGCAACAACAGCAATCCAAGCCAAACTTTCTGATTTTGGATAACTTCAACTTCGAGACTGTTTCTTCTCAACATGGTTTTTATCATGCCAATAAGGCCGGGAAGGATCACCAACACCACCATCACCAAGAACAGCAACAATATTCGAAGTCAAGTTCTTCAAATTTGGAGAATTTCAGCAGCTTTGATTTTACCTTTCCCTCAGGGCAAGTTACACAACCAGTTGAGGATGGTGCTCGGTTTACACACAGCCAGATAGGAGGCTCAGACATAGTTGAAACTATCAAGAAAATGCCGCCTCCTCAATCTTCTCTAGCAGCACTGGAGCTCCTAAACAGTTATGGAAGCGCCTTCAAGAAGTTGAGGGGGGAAAGACAAAGAAGTAGCAAGATAGTTAAAGGCAAAGAAATTGATCAAAACAGCAGCTATGTTGTTAGTGGTAAACAGAAACTATCAATCGAAGAGGTCATGAGGATTGCTGGAGCAAGGTTTGTGCAATTCTCTAGTCAGGGGTATGAAGACTTTTACATGTCTTTGCACCCTTTTGGCTATGCACTTTCAGGTCTTTGCGAAGAAGAAACGAAAGATGTAGAACTTGCTCACTTACTCTTAGCTGCAGCAGAGAAAGTAGGGTACCAGCAATTTGAAAGAGCAAGCAGATTACTTCTGCAGTGTGAATGGCTTGCATCCTTCAAAGGCACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCGGAGGCACTTCGTGAGAGGATTGAAAAAGAAACAGGACTCTATACATCTAAGGGAAATGAAGAAATGTCTCATTATGTACATGGTTTGGGGACAAACCTTGCATTCATCGCATGCCACCAAGAAGTCCCTTTCCATCCAGTACTGCAGTTCGCAGCAATCCAAACCATGATAGAAAATGTTGCAAATGAGAGTAGGATCCATTTAATTGATCTTGAAATCAGGTTTGGTACGCAATGGACAGGGTTGATGGAAGCACTAGCAGAACGTGAGGAATGCCCGGTTGAGCTTCTTACTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGACAAGTGTTGCAATGTCCTTGAACCTACCCTTTCAATTTAAGGAAGTTATTGTTTCAGACATGCAAGACATTAATGACCGTTTATTCGATGAAATTGAAGATGACCAAGCTTTGGTTGTGTACGCGCCATTAGTACTGAGGACAATGATACCAAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATACCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCCCCATCGTTTGTGAACCGTTTCATTGATGCATTGTTTTACCATAGTGCATTTTTCGATTGCGTTGAAACCTGTATGACAGAGGGGAAGGGTAGGGTGATCATGGAAAGCTTGTTGCGAGAGGGAATCCGGAACATAGTGGCGGCTGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCGAGGTTTAGAATGGTGGAAATGAGCTTTAGTAACACATCATTGTATCAAGCTAATGAGATTGCTAAAAAGATTAAATTACTACTAATAATCGGGCTTCGGACGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

636

Amino Acids

72.29

Weight (kDa)

5.68

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 290 - 625 1e-67 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 903
AccB7I CCANNNNNTGG 1 cut(s) 1465
AccII CGCG 2 cut(s) 1001, 1478
AccIII TCCGGA 1 cut(s) 1725
AciI CCGC 4 cut(s) 51, 566, 1001, 1739
AclWI GGATC 3 cut(s) 384, 1192, 1205
AcsI RAATTY 2 cut(s) 433, 442
AcuI CTGAAG 1 cut(s) 1764
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 7 cut(s) 23, 905, 1080, 1143, 1231, 1475, 1488
AfiI CCNNNNNNNGG 1 cut(s) 1465
AflIII ACRYGT 1 cut(s) 798
AjuI GAANNNNNNNTTGG 2 cut(s) 1209, 1241
Alw21I GWGCWC 2 cut(s) 508, 600
Alw26I GTCTC 3 cut(s) 320, 1074, 1331
AlwI GGATC 3 cut(s) 384, 1192, 1205
Aor13HI TCCGGA 1 cut(s) 1725
AoxI GGCC 4 cut(s) 201, 366, 1508, 1791
ApeKI GCWGC 6 cut(s) 450, 587, 693, 884, 887, 1154
ApoI RAATTY 2 cut(s) 433, 442
ArsI GACNNNNNNTTYG 2 cut(s) 1421, 1453
AseI ATTAAT 1 cut(s) 1425
Asp700I GAANNNNTTC 1 cut(s) 1616
Asp718I GGTACC 1 cut(s) 903
AspA2I CCTAGG 1 cut(s) 1511
AspLEI GCGC 2 cut(s) 620, 1480
AspS9I GGNCC 1 cut(s) 1791
AsuC2I CCSGG 2 cut(s) 370, 1280
AsuHPI GGTGA 4 cut(s) 371, 386, 1549, 1705
AsuII TTCGAA 2 cut(s) 39, 416
AvrII CCTAGG 1 cut(s) 1511
AxyI CCTNAGG 1 cut(s) 472
BamHI GGATCC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 903
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 96, 795
Bbv12I GWGCWC 2 cut(s) 508, 600
BbvI GCAGC 6 cut(s) 462, 599, 705, 871, 899, 1166
BccI CCATC 7 cut(s) 399, 494, 1143, 1243, 1558, 1609, 1771
BcgI CGANNNNNNTGC 1 cut(s) 34
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 2 cut(s) 682, 1695
BcnI CCSGG 2 cut(s) 370, 1280
BcoDI GTCTC 3 cut(s) 320, 1074, 1331
BfaI CTAG 6 cut(s) 137, 188, 584, 774, 1259, 1512
BfmI CTRYAG 3 cut(s) 885, 938, 1145
BfoI RGCGCY 1 cut(s) 621
BfuI GTATCC 1 cut(s) 232
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 8 cut(s) 451, 566, 588, 694, 885, 888, 1155, 1740
BlnI CCTAGG 1 cut(s) 1511
BlsI GCNGC 8 cut(s) 452, 567, 589, 695, 886, 889, 1156, 1741
BmcAI AGTACT 2 cut(s) 1143, 1488
Bme1390I CCNGG 2 cut(s) 370, 1280
BmgT120I GGNCC 1 cut(s) 1791
BmiI GGNNCC 2 cut(s) 905, 1199
BmrFI CCNGG 2 cut(s) 370, 1280
BmsI GCATC 2 cut(s) 965, 1615
BpiI GAAGAC 2 cut(s) 96, 795
BpmI CTGGAG 2 cut(s) 614, 771
Bpu14I TTCGAA 2 cut(s) 39, 416
BpuMI CCSGG 2 cut(s) 370, 1280
BsaJI CCNNGG 3 cut(s) 46, 1505, 1511
BsaWI WCCGGW 1 cut(s) 1725
BsaXI ACNNNNNCTCC 6 cut(s) 204, 234, 582, 612, 1580, 1610
Bsc4I CCNNNNNNNGG 1 cut(s) 1465
Bse1I ACTGG 3 cut(s) 491, 597, 1139
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 2 cut(s) 1241, 1371
BseAI TCCGGA 1 cut(s) 1725
BseDI CCNNGG 3 cut(s) 46, 1505, 1511
BseGI GGATG 4 cut(s) 505, 956, 1135, 1786
BseLI CCNNNNNNNGG 1 cut(s) 1465
BseMI GCAATG 2 cut(s) 1241, 1371
BseMII CTCAG 3 cut(s) 486, 549, 1481
BseNI ACTGG 3 cut(s) 491, 597, 1139
BseRI GAGGAG 1 cut(s) 561
BseXI GCAGC 6 cut(s) 462, 599, 705, 871, 899, 1166
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 1001, 1478
BshFI GGCC 4 cut(s) 203, 368, 1510, 1793
BshNI GGYRCC 1 cut(s) 903
BsiHKAI GWGCWC 2 cut(s) 508, 600
BsiSI CCGG 3 cut(s) 369, 1280, 1726
BslFI GGGAC 2 cut(s) 1105, 1112
BslI CCNNNNNNNGG 1 cut(s) 1465
BsmAI GTCTC 3 cut(s) 320, 1074, 1331
BsmFI GGGAC 2 cut(s) 1105, 1112
BsmI GAATGC 3 cut(s) 11, 1103, 1280
BsnI GGCC 4 cut(s) 203, 368, 1510, 1793
Bsp119I TTCGAA 2 cut(s) 39, 416
Bsp1286I GDGCHC 2 cut(s) 508, 600
Bsp13I TCCGGA 1 cut(s) 1725
Bsp1407I TGTACA 1 cut(s) 1078
Bsp143I GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
BspACI CCGC 4 cut(s) 51, 566, 1001, 1739
BspANI GGCC 4 cut(s) 203, 368, 1510, 1793
BspCNI CTCAG 3 cut(s) 485, 548, 1482
BspEI TCCGGA 1 cut(s) 1725
BspFNI CGCG 2 cut(s) 1001, 1478
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 2 cut(s) 905, 1199
BspMAI CTGCAG 3 cut(s) 889, 942, 1149
BspPI GGATC 3 cut(s) 384, 1192, 1205
BspT104I TTCGAA 2 cut(s) 39, 416
BspT107I GGYRCC 1 cut(s) 903
BsrDI GCAATG 2 cut(s) 1241, 1371
BsrGI TGTACA 1 cut(s) 1078
BsrI ACTGG 3 cut(s) 491, 597, 1139
BssECI CCNNGG 3 cut(s) 46, 1505, 1511
BssMI GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
BssT1I CCWWGG 3 cut(s) 46, 1505, 1511
Bst4CI ACNGT 5 cut(s) 97, 331, 608, 1433, 1616
Bst6I CTCTTC 1 cut(s) 726
BstAUI TGTACA 1 cut(s) 1078
BstBI TTCGAA 2 cut(s) 39, 416
BstC8I GCNNGC 3 cut(s) 925, 954, 1114
BstDEI CTNAG 5 cut(s) 472, 535, 880, 1050, 1490
BstF5I GGATG 4 cut(s) 505, 956, 1135, 1786
BstFNI CGCG 2 cut(s) 1001, 1478
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 2 cut(s) 620, 1480
BstKTI GATC 9 cut(s) 124, 145, 241, 259, 379, 685, 1200, 1213, 1698
BstMAI GTCTC 3 cut(s) 320, 1074, 1331
BstMBI GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
BstMWI GCNNNNNNNGC 5 cut(s) 280, 289, 365, 1109, 1799
BstNSI RCATGY 3 cut(s) 802, 1116, 1417
BstSCI CCNGG 2 cut(s) 368, 1278
BstSFI CTRYAG 3 cut(s) 885, 938, 1145
BstUI CGCG 2 cut(s) 1001, 1478
BstV1I GCAGC 6 cut(s) 462, 599, 705, 871, 899, 1166
BstV2I GAAGAC 2 cut(s) 96, 795
BstX2I RGATCY 1 cut(s) 1197
BstYI RGATCY 1 cut(s) 1197
Bsu36I CCTNAGG 1 cut(s) 472
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 4 cut(s) 203, 368, 1510, 1793
BtgZI GCGATG 1 cut(s) 1093
BtsCI GGATG 4 cut(s) 505, 956, 1135, 1786
BtsI GCAGTG 1 cut(s) 947
BtsIMutI CAGTG 2 cut(s) 590, 947
Cac8I GCNNGC 3 cut(s) 925, 954, 1114
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 620, 1480
Cfr13I GGNCC 1 cut(s) 1791
Csp6I GTAC 7 cut(s) 22, 904, 1079, 1142, 1230, 1474, 1487
CviQI GTAC 7 cut(s) 22, 904, 1079, 1142, 1230, 1474, 1487
DdeI CTNAG 5 cut(s) 472, 535, 880, 1050, 1490
DpnI GATC 9 cut(s) 123, 144, 240, 258, 378, 684, 1199, 1212, 1697
DpnII GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
DraIII CACNNNGTG 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 3 cut(s) 46, 1505, 1511
Eco147I AGGCCT 1 cut(s) 1510
Eco24I GRGCYC 1 cut(s) 600
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 1764
Eco81I CCTNAGG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 1791
EcoT14I CCWWGG 3 cut(s) 46, 1505, 1511
EcoT22I ATGCAT 2 cut(s) 13, 1630
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 3 cut(s) 46, 1505, 1511
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 2 cut(s) 1105, 1112
FbaI TGATCA 2 cut(s) 682, 1695
Fnu4HI GCNGC 8 cut(s) 451, 566, 588, 694, 885, 888, 1155, 1740
FokI GGATG 4 cut(s) 512, 943, 1122, 1793
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 8 cut(s) 451, 566, 588, 694, 885, 888, 1155, 1740
FspBI CTAG 6 cut(s) 137, 188, 584, 774, 1259, 1512
GlaI GCGC 2 cut(s) 619, 1479
GluI GCNGC 8 cut(s) 451, 566, 588, 694, 885, 888, 1155, 1740
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 614, 771
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 4 cut(s) 203, 368, 1510, 1793
HapII CCGG 3 cut(s) 369, 1280, 1726
HhaI GCGC 2 cut(s) 620, 1480
Hin6I GCGC 2 cut(s) 618, 1478
HinP1I GCGC 2 cut(s) 618, 1478
HincII GTYRAC 1 cut(s) 1353
HindII GTYRAC 1 cut(s) 1353
HindIII AAGCTT 2 cut(s) 1461, 1705
HinfI GANTC 3 cut(s) 58, 1038, 1722
HpaII CCGG 3 cut(s) 369, 1280, 1726
HphI GGTGA 4 cut(s) 371, 386, 1549, 1705
Hpy166II GTNNAC 7 cut(s) 24, 62, 513, 713, 1353, 1474, 1612
Hpy188I TCNGA 6 cut(s) 172, 303, 538, 981, 1411, 1902
Hpy188III TCNNGA 9 cut(s) 137, 260, 325, 556, 625, 739, 1013, 1214, 1726
Hpy8I GTNNAC 7 cut(s) 24, 62, 513, 713, 1353, 1474, 1612
HpyAV CCTTC 8 cut(s) 177, 367, 631, 970, 1566, 1678, 1739, 1804
HpyCH4III ACNGT 5 cut(s) 97, 331, 608, 1433, 1616
HpyCH4IV ACGT 1 cut(s) 1267
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 289, 365, 1109, 1799
HpyF3I CTNAG 5 cut(s) 472, 535, 880, 1050, 1490
HpySE526I ACGT 1 cut(s) 1267
HspAI GCGC 2 cut(s) 618, 1478
Kpn2I TCCGGA 1 cut(s) 1725
KpnI GGTACC 1 cut(s) 907
Ksp22I TGATCA 2 cut(s) 682, 1695
Kzo9I GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
LmnI GCTCC 3 cut(s) 595, 603, 752
Lsp1109I GCAGC 6 cut(s) 462, 599, 705, 871, 899, 1166
LweI GCATC 2 cut(s) 965, 1615
MaeI CTAG 6 cut(s) 137, 188, 584, 774, 1259, 1512
MaeII ACGT 1 cut(s) 1267
MaeIII GTNAC 2 cut(s) 481, 1832
MalI GATC 9 cut(s) 123, 144, 240, 258, 378, 684, 1199, 1212, 1697
MboI GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
MflI RGATCY 1 cut(s) 1197
MhlI GDGCHC 2 cut(s) 508, 600
MluCI AATT 9 cut(s) 433, 442, 678, 767, 911, 1206, 1388, 1446, 1877
MlyI GAGTC 2 cut(s) 67, 1032
Mph1103I ATGCAT 2 cut(s) 13, 1630
MroI TCCGGA 1 cut(s) 1725
MroXI GAANNNNTTC 1 cut(s) 1616
MseI TTAA 5 cut(s) 666, 1205, 1392, 1425, 1875
MslI CAYNNNNRTG 2 cut(s) 98, 1577
MspI CCGG 3 cut(s) 369, 1280, 1726
MspR9I CCNGG 2 cut(s) 370, 1280
Mva1269I GAATGC 3 cut(s) 11, 1103, 1280
MvnI CGCG 2 cut(s) 1001, 1478
MwoI GCNNNNNNNGC 5 cut(s) 280, 289, 365, 1109, 1799
NciI CCSGG 2 cut(s) 370, 1280
NdeII GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
NlaIV GGNNCC 2 cut(s) 905, 1199
NsiI ATGCAT 2 cut(s) 13, 1630
NspI RCATGY 3 cut(s) 802, 1116, 1417
NspV TTCGAA 2 cut(s) 39, 416
PaeI GCATGC 1 cut(s) 1116
PagI TCATGA 1 cut(s) 738
PceI AGGCCT 1 cut(s) 1510
PciI ACATGT 1 cut(s) 798
PctI GAATGC 3 cut(s) 11, 1103, 1280
PdmI GAANNNNTTC 1 cut(s) 1616
PfeI GAWTC 1 cut(s) 1722
PflMI CCANNNNNTGG 1 cut(s) 1465
PkrI GCNGC 8 cut(s) 452, 567, 589, 695, 886, 889, 1156, 1741
PleI GAGTC 2 cut(s) 66, 1032
PpsI GAGTC 2 cut(s) 66, 1032
PscI ACATGT 1 cut(s) 798
PshBI ATTAAT 1 cut(s) 1425
Psp124BI GAGCTC 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 905, 1199
PspPI GGNCC 1 cut(s) 1791
PstI CTGCAG 3 cut(s) 889, 942, 1149
PsuI RGATCY 1 cut(s) 1197
RsaI GTAC 7 cut(s) 23, 905, 1080, 1143, 1231, 1475, 1488
RsaNI GTAC 7 cut(s) 22, 904, 1079, 1142, 1230, 1474, 1487
RseI CAYNNNNRTG 2 cut(s) 98, 1577
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 5 cut(s) 666, 1205, 1392, 1425, 1875
SatI GCNGC 8 cut(s) 451, 566, 588, 694, 885, 888, 1155, 1740
Sau3AI GATC 9 cut(s) 121, 142, 238, 256, 376, 682, 1197, 1210, 1695
Sau96I GGNCC 1 cut(s) 1791
ScaI AGTACT 2 cut(s) 1143, 1488
SchI GAGTC 2 cut(s) 67, 1032
ScrFI CCNGG 2 cut(s) 370, 1280
SduI GDGCHC 2 cut(s) 508, 600
SfaNI GCATC 2 cut(s) 965, 1615
SfcI CTRYAG 3 cut(s) 885, 938, 1145
SfuI TTCGAA 2 cut(s) 39, 416
SmiMI CAYNNNNRTG 2 cut(s) 98, 1577
SphI GCATGC 1 cut(s) 1116
Sse9I AATT 9 cut(s) 433, 442, 678, 767, 911, 1206, 1388, 1446, 1877
SseBI AGGCCT 1 cut(s) 1510
SsiI CCGC 4 cut(s) 51, 566, 1001, 1739
SspI AATATT 1 cut(s) 413
SspMI CTAG 6 cut(s) 137, 188, 584, 774, 1259, 1512
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 1 cut(s) 1510
StyD4I CCNGG 2 cut(s) 368, 1278
StyI CCWWGG 3 cut(s) 46, 1505, 1511
TaaI ACNGT 5 cut(s) 97, 331, 608, 1433, 1616
TaiI ACGT 1 cut(s) 1270
TaqI TCGA 7 cut(s) 39, 324, 416, 729, 1440, 1653, 1765
TasI AATT 9 cut(s) 433, 442, 678, 767, 911, 1206, 1388, 1446, 1877
TatI WGTACW 3 cut(s) 1078, 1141, 1486
TauI GCSGC 2 cut(s) 568, 1742
TfiI GAWTC 1 cut(s) 1722
Tru1I TTAA 5 cut(s) 666, 1205, 1392, 1425, 1875
Tru9I TTAA 5 cut(s) 666, 1205, 1392, 1425, 1875
TscAI CASTG 2 cut(s) 597, 947
TseI GCWGC 6 cut(s) 450, 587, 693, 884, 887, 1154
TspDTI ATGAA 6 cut(s) 173, 801, 1074, 1096, 1458, 1609
TspRI CASTG 2 cut(s) 597, 947
Van91I CCANNNNNTGG 1 cut(s) 1465
VspI ATTAAT 1 cut(s) 1425
XapI RAATTY 2 cut(s) 433, 442
XbaI TCTAGA 1 cut(s) 136
XceI RCATGY 3 cut(s) 802, 1116, 1417
XmaJI CCTAGG 1 cut(s) 1511
XmnI GAANNNNTTC 1 cut(s) 1616
XspI CTAG 6 cut(s) 137, 188, 584, 774, 1259, 1512
ZrmI AGTACT 2 cut(s) 1143, 1488
Zsp2I ATGCAT 2 cut(s) 13, 1630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.