Rroxscaffold_3G00219290

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005917
Physical Location & Seq
Reverse (-)
1441155 .. 1443163
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219290.1

Sequence Viewer

Length: 1623 bp
ATGGCAAATGCAGTCTTCTCGTTCTCACCATTTGATTTCGAAATCCAAGGCGGTTTGAGTCCACCCCAGCTTGAAGACTATGACAGAGAAGACACAGTAATGTTCAATGTTCACAGGGGAGACCATTTGTTTGGTCTAGAAGATCAGTTGGGAGAAATAAATACTCCTTCTGAACATGGACAAGTTACACAACCAGTTGAGGATGGTGCTCGGTTTACACACAGCCAGATAGGAGGCTTAGACATAGTTGAAACTATCAAGAAAATGCCGCCTCCTCAATCTTCTCTAGCAACACTGGAGCTCCTAAACAGTTATGGAAGCGCCTTCAAGAAGTTGAGGGGGGAAAGACAAAGAAGTAGCAAGATAGTTAAAGGCAAAGAAATTGATCAAAACAGCAGCTATGTTGTTAGTGGTAAACAGAAACTATCAATCGAAGAGGTCATGAGGGTTGCTGGAGCAAGGTTTGTGCAATTCTCTAGTCAGGGGTATGAAGACTTTTACATGTCTATGCACCCTTTTGGCTATGCACTTTCAGGTCTTTGCGAAGAAGAAACGAAAGATGTAGAACTTGCTCACTTACTCTTAGCTGCAGCAGAGAAAGTAGGGTACCAGCAATTTGAAAGAGCAATCAGATTACTTCTGCAGTGTGAATGGCTTGCATCCTTCAAAAACACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCGGAGGCACTTCGTGAGAGGATTGAAAAAGAAACAGGACTCTATACATCTAAGGGAAATGAAGAAATGTCTCATTATGTACATGGTTTGGGGACAAACCTTGCATTCATCGCATGCCACCAAGAAGTCCCTTTCCATCCAGTACTGCAGTTCGCGGCAATCCAAGCCATGATAGAAAATGTTGCAAATGAGAGTAGGATCCATTTAATTGATCTTGAAATCAGGTTTGGTACGCAATGGACAGGGTTGATGGAAGCACTAGCAGAACGTGAGGAATGCCCGGTTGAGCTTCTTACTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGACGAGTGTTGCAATGTCCTTGAACCTACCCTTTCAATTTAAGGAAGTTATTGTTTCAGACATGCAAGACATTAATGACCGTTTATTCTATGAAATTGAAGTTGACCAAGCTTTGGTTGTGTACGCGCCATTAGTACTGAGGACAATGATAGCAAGGCCTAGGTGCTTGGAGAACTTGATGAGAGTGATGAGAAATATCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCACCATCGTTTGTGAACCGTTTCATTGATGCATTGTTTTACCATAGTGCATTTTTCGATTGCGTTGAAACCTGTATGACAGAGGGGAAGGGTAGGGTGATCATGGAAGGCTTGTTGCGAGAGGGAATCCGGAACATAGTGGCAGCTGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCGAGGTTTAGAATGGTGGAAATGAGCTTTAGTAACACATCATTGTATCAAGCTAATGAGATTGCTAAAAAGAGAAAGAGAGATGGTCTTCAAAACAAATGTAACATAGGCTTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

540

Amino Acids

61.21

Weight (kDa)

5.54

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 191 - 532 2.7e-68 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 606
AccB1I GGYRCC 1 cut(s) 606
AccB7I CCANNNNNTGG 1 cut(s) 1168
AccII CGCG 3 cut(s) 704, 857, 1181
AccIII TCCGGA 1 cut(s) 1428
AciI CCGC 4 cut(s) 51, 269, 704, 857
AclWI GGATC 2 cut(s) 895, 908
AcuI CTGAAG 1 cut(s) 1467
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 6 cut(s) 608, 783, 846, 934, 1178, 1191
AfiI CCNNNNNNNGG 1 cut(s) 1168
AflIII ACRYGT 1 cut(s) 501
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
Alw21I GWGCWC 2 cut(s) 211, 303
Alw26I GTCTC 3 cut(s) 114, 777, 1034
AlwI GGATC 2 cut(s) 895, 908
Aor13HI TCCGGA 1 cut(s) 1428
AoxI GGCC 2 cut(s) 1211, 1494
ApeKI GCWGC 4 cut(s) 396, 587, 590, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AseI ATTAAT 1 cut(s) 1128
Asp700I GAANNNNTTC 1 cut(s) 1319
Asp718I GGTACC 1 cut(s) 606
AspA2I CCTAGG 1 cut(s) 1214
AspLEI GCGC 2 cut(s) 323, 1183
AspS9I GGNCC 1 cut(s) 1494
AsuC2I CCSGG 1 cut(s) 983
AsuHPI GGTGA 3 cut(s) 18, 1293, 1408
AsuII TTCGAA 1 cut(s) 39
AvrII CCTAGG 1 cut(s) 1214
BamHI GGATCC 1 cut(s) 900
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 5 cut(s) 7, 81, 96, 498, 1583
Bbv12I GWGCWC 2 cut(s) 211, 303
BbvI GCAGC 4 cut(s) 408, 574, 602, 1454
BccI CCATC 7 cut(s) 197, 846, 946, 1261, 1312, 1474, 1580
BcgI CGANNNNNNTGC 1 cut(s) 34
BclI TGATCA 2 cut(s) 385, 1398
BcnI CCSGG 1 cut(s) 983
BcoDI GTCTC 3 cut(s) 114, 777, 1034
BfaI CTAG 5 cut(s) 137, 287, 477, 962, 1215
BfmI CTRYAG 3 cut(s) 588, 641, 848
BfoI RGCGCY 1 cut(s) 324
BisI GCNGC 6 cut(s) 269, 397, 588, 591, 858, 1443
BlnI CCTAGG 1 cut(s) 1214
BlsI GCNGC 6 cut(s) 270, 398, 589, 592, 859, 1444
BmcAI AGTACT 2 cut(s) 846, 1191
Bme1390I CCNGG 1 cut(s) 983
BmgT120I GGNCC 1 cut(s) 1494
BmiI GGNNCC 2 cut(s) 608, 902
BmrFI CCNGG 1 cut(s) 983
BmsI GCATC 2 cut(s) 668, 1318
BpiI GAAGAC 5 cut(s) 7, 81, 96, 498, 1583
BpmI CTGGAG 2 cut(s) 317, 474
Bpu14I TTCGAA 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 46, 1214
BsaWI WCCGGW 1 cut(s) 1428
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 1168
Bse1I ACTGG 3 cut(s) 194, 300, 842
Bse3DI GCAATG 2 cut(s) 944, 1074
BseAI TCCGGA 1 cut(s) 1428
BseDI CCNNGG 2 cut(s) 46, 1214
BseGI GGATG 4 cut(s) 208, 659, 838, 1489
BseLI CCNNNNNNNGG 1 cut(s) 1168
BseMI GCAATG 2 cut(s) 944, 1074
BseMII CTCAG 1 cut(s) 1184
BseNI ACTGG 3 cut(s) 194, 300, 842
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 4 cut(s) 408, 574, 602, 1454
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 3 cut(s) 704, 857, 1181
BshFI GGCC 2 cut(s) 1213, 1496
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 211, 303
BsiSI CCGG 2 cut(s) 983, 1429
BslFI GGGAC 2 cut(s) 808, 815
BslI CCNNNNNNNGG 1 cut(s) 1168
BsmAI GTCTC 3 cut(s) 114, 777, 1034
BsmFI GGGAC 2 cut(s) 808, 815
BsmI GAATGC 2 cut(s) 806, 983
BsnI GGCC 2 cut(s) 1213, 1496
Bso31I GGTCTC 1 cut(s) 114
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 211, 303
Bsp13I TCCGGA 1 cut(s) 1428
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 5 cut(s) 142, 385, 900, 913, 1398
BspACI CCGC 4 cut(s) 51, 269, 704, 857
BspANI GGCC 2 cut(s) 1213, 1496
BspCNI CTCAG 1 cut(s) 1185
BspEI TCCGGA 1 cut(s) 1428
BspFNI CGCG 3 cut(s) 704, 857, 1181
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 608, 902
BspMAI CTGCAG 3 cut(s) 592, 645, 852
BspPI GGATC 2 cut(s) 895, 908
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 606
BspTNI GGTCTC 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 944, 1074
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 3 cut(s) 194, 300, 842
BssECI CCNNGG 2 cut(s) 46, 1214
BssMI GATC 5 cut(s) 142, 385, 900, 913, 1398
BssT1I CCWWGG 2 cut(s) 46, 1214
Bst4CI ACNGT 4 cut(s) 97, 311, 1136, 1319
Bst6I CTCTTC 1 cut(s) 429
BstAUI TGTACA 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 657, 817
BstDEI CTNAG 4 cut(s) 238, 583, 753, 1193
BstF5I GGATG 4 cut(s) 208, 659, 838, 1489
BstFNI CGCG 3 cut(s) 704, 857, 1181
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 2 cut(s) 323, 1183
BstKTI GATC 5 cut(s) 145, 388, 903, 916, 1401
BstMAI GTCTC 3 cut(s) 114, 777, 1034
BstMBI GATC 5 cut(s) 142, 385, 900, 913, 1398
BstMWI GCNNNNNNNGC 3 cut(s) 812, 866, 1502
BstNSI RCATGY 3 cut(s) 505, 819, 1120
BstSCI CCNGG 1 cut(s) 981
BstSFI CTRYAG 3 cut(s) 588, 641, 848
BstUI CGCG 3 cut(s) 704, 857, 1181
BstV1I GCAGC 4 cut(s) 408, 574, 602, 1454
BstV2I GAAGAC 5 cut(s) 7, 81, 96, 498, 1583
BstX2I RGATCY 1 cut(s) 900
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 900
BsuRI GGCC 2 cut(s) 1213, 1496
BtgZI GCGATG 1 cut(s) 796
BtsCI GGATG 4 cut(s) 208, 659, 838, 1489
BtsI GCAGTG 1 cut(s) 650
BtsIMutI CAGTG 2 cut(s) 293, 650
Cac8I GCNNGC 2 cut(s) 657, 817
CciI TCATGA 1 cut(s) 441
CfoI GCGC 2 cut(s) 323, 1183
Cfr13I GGNCC 1 cut(s) 1494
Csp6I GTAC 6 cut(s) 607, 782, 845, 933, 1177, 1190
CviAII CATG 8 cut(s) 176, 442, 502, 785, 816, 871, 1117, 1402
CviQI GTAC 6 cut(s) 607, 782, 845, 933, 1177, 1190
DdeI CTNAG 4 cut(s) 238, 583, 753, 1193
DpnI GATC 5 cut(s) 144, 387, 902, 915, 1400
DpnII GATC 5 cut(s) 142, 385, 900, 913, 1398
DraIII CACNNNGTG 1 cut(s) 716
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 46, 1214
Eco147I AGGCCT 1 cut(s) 1213
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 1467
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 1494
EcoT14I CCWWGG 2 cut(s) 46, 1214
EcoT22I ATGCAT 1 cut(s) 1333
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 46, 1214
FaeI CATG 8 cut(s) 179, 445, 505, 788, 819, 874, 1120, 1405
FaqI GGGAC 2 cut(s) 808, 815
FatI CATG 8 cut(s) 175, 441, 501, 784, 815, 870, 1116, 1401
FbaI TGATCA 2 cut(s) 385, 1398
Fnu4HI GCNGC 6 cut(s) 269, 397, 588, 591, 858, 1443
FokI GGATG 4 cut(s) 215, 646, 825, 1496
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 6 cut(s) 269, 397, 588, 591, 858, 1443
FspBI CTAG 5 cut(s) 137, 287, 477, 962, 1215
GlaI GCGC 2 cut(s) 322, 1182
GluI GCNGC 6 cut(s) 269, 397, 588, 591, 858, 1443
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 317, 474
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 2 cut(s) 1213, 1496
HapII CCGG 2 cut(s) 983, 1429
HhaI GCGC 2 cut(s) 323, 1183
Hin1II CATG 8 cut(s) 179, 445, 505, 788, 819, 874, 1120, 1405
Hin6I GCGC 2 cut(s) 321, 1181
HinP1I GCGC 2 cut(s) 321, 1181
HincII GTYRAC 2 cut(s) 1056, 1159
HindII GTYRAC 2 cut(s) 1056, 1159
HindIII AAGCTT 1 cut(s) 1164
HinfI GANTC 3 cut(s) 58, 741, 1425
HpaII CCGG 2 cut(s) 983, 1429
HphI GGTGA 3 cut(s) 18, 1293, 1408
Hpy166II GTNNAC 8 cut(s) 62, 112, 216, 416, 1056, 1159, 1177, 1315
Hpy188I TCNGA 4 cut(s) 172, 632, 684, 1114
Hpy188III TCNNGA 7 cut(s) 137, 259, 328, 442, 716, 917, 1429
Hpy8I GTNNAC 8 cut(s) 62, 112, 216, 416, 1056, 1159, 1177, 1315
HpyAV CCTTC 8 cut(s) 177, 334, 673, 1269, 1381, 1400, 1442, 1507
HpyCH4III ACNGT 4 cut(s) 97, 311, 1136, 1319
HpyCH4IV ACGT 1 cut(s) 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 812, 866, 1502
HpyF3I CTNAG 4 cut(s) 238, 583, 753, 1193
HpySE526I ACGT 1 cut(s) 970
Hsp92II CATG 8 cut(s) 179, 445, 505, 788, 819, 874, 1120, 1405
HspAI GCGC 2 cut(s) 321, 1181
Kpn2I TCCGGA 1 cut(s) 1428
KpnI GGTACC 1 cut(s) 610
Ksp22I TGATCA 2 cut(s) 385, 1398
Kzo9I GATC 5 cut(s) 142, 385, 900, 913, 1398
LmnI GCTCC 3 cut(s) 298, 306, 455
Lsp1109I GCAGC 4 cut(s) 408, 574, 602, 1454
LweI GCATC 2 cut(s) 668, 1318
MaeI CTAG 5 cut(s) 137, 287, 477, 962, 1215
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 3 cut(s) 184, 1535, 1604
MalI GATC 5 cut(s) 144, 387, 902, 915, 1400
MboI GATC 5 cut(s) 142, 385, 900, 913, 1398
MflI RGATCY 1 cut(s) 900
MhlI GDGCHC 2 cut(s) 211, 303
MluCI AATT 6 cut(s) 381, 470, 614, 909, 1091, 1149
MlyI GAGTC 2 cut(s) 67, 735
Mph1103I ATGCAT 1 cut(s) 1333
MroI TCCGGA 1 cut(s) 1428
MroXI GAANNNNTTC 1 cut(s) 1319
MseI TTAA 4 cut(s) 369, 908, 1095, 1128
MslI CAYNNNNRTG 3 cut(s) 98, 506, 1280
MspA1I CMGCKG 1 cut(s) 1445
MspI CCGG 2 cut(s) 983, 1429
MspR9I CCNGG 1 cut(s) 983
Mva1269I GAATGC 2 cut(s) 806, 983
MvnI CGCG 3 cut(s) 704, 857, 1181
MwoI GCNNNNNNNGC 3 cut(s) 812, 866, 1502
NciI CCSGG 1 cut(s) 983
NdeII GATC 5 cut(s) 142, 385, 900, 913, 1398
NlaIII CATG 8 cut(s) 179, 445, 505, 788, 819, 874, 1120, 1405
NlaIV GGNNCC 2 cut(s) 608, 902
NsiI ATGCAT 1 cut(s) 1333
NspI RCATGY 3 cut(s) 505, 819, 1120
NspV TTCGAA 1 cut(s) 39
PaeI GCATGC 1 cut(s) 819
PagI TCATGA 1 cut(s) 441
PceI AGGCCT 1 cut(s) 1213
PciI ACATGT 1 cut(s) 501
PctI GAATGC 2 cut(s) 806, 983
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 1 cut(s) 1425
PflMI CCANNNNNTGG 1 cut(s) 1168
PkrI GCNGC 6 cut(s) 270, 398, 589, 592, 859, 1444
PleI GAGTC 2 cut(s) 66, 735
PpsI GAGTC 2 cut(s) 66, 735
PscI ACATGT 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 1128
Psp124BI GAGCTC 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 608, 902
PspPI GGNCC 1 cut(s) 1494
PstI CTGCAG 3 cut(s) 592, 645, 852
PsuI RGATCY 1 cut(s) 900
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 6 cut(s) 608, 783, 846, 934, 1178, 1191
RsaNI GTAC 6 cut(s) 607, 782, 845, 933, 1177, 1190
RseI CAYNNNNRTG 3 cut(s) 98, 506, 1280
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 4 cut(s) 369, 908, 1095, 1128
SatI GCNGC 6 cut(s) 269, 397, 588, 591, 858, 1443
Sau3AI GATC 5 cut(s) 142, 385, 900, 913, 1398
Sau96I GGNCC 1 cut(s) 1494
ScaI AGTACT 2 cut(s) 846, 1191
SchI GAGTC 2 cut(s) 67, 735
ScrFI CCNGG 1 cut(s) 983
SduI GDGCHC 2 cut(s) 211, 303
SfaNI GCATC 2 cut(s) 668, 1318
SfcI CTRYAG 3 cut(s) 588, 641, 848
SfuI TTCGAA 1 cut(s) 39
SmiMI CAYNNNNRTG 3 cut(s) 98, 506, 1280
SphI GCATGC 1 cut(s) 819
Sse9I AATT 6 cut(s) 381, 470, 614, 909, 1091, 1149
SseBI AGGCCT 1 cut(s) 1213
SsiI CCGC 4 cut(s) 51, 269, 704, 857
SspMI CTAG 5 cut(s) 137, 287, 477, 962, 1215
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 1213
StyD4I CCNGG 1 cut(s) 981
StyI CCWWGG 2 cut(s) 46, 1214
TaaI ACNGT 4 cut(s) 97, 311, 1136, 1319
TaiI ACGT 1 cut(s) 973
TaqI TCGA 4 cut(s) 39, 432, 1356, 1468
TasI AATT 6 cut(s) 381, 470, 614, 909, 1091, 1149
TatI WGTACW 3 cut(s) 781, 844, 1189
TauI GCSGC 2 cut(s) 271, 860
TfiI GAWTC 1 cut(s) 1425
Tru1I TTAA 4 cut(s) 369, 908, 1095, 1128
Tru9I TTAA 4 cut(s) 369, 908, 1095, 1128
TscAI CASTG 2 cut(s) 300, 650
TseI GCWGC 4 cut(s) 396, 587, 590, 1442
TspDTI ATGAA 5 cut(s) 504, 777, 799, 1161, 1312
TspRI CASTG 2 cut(s) 300, 650
Van91I CCANNNNNTGG 1 cut(s) 1168
VspI ATTAAT 1 cut(s) 1128
XbaI TCTAGA 1 cut(s) 136
XceI RCATGY 3 cut(s) 505, 819, 1120
XmaJI CCTAGG 1 cut(s) 1214
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 5 cut(s) 137, 287, 477, 962, 1215
ZrmI AGTACT 2 cut(s) 846, 1191
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.