RchiOBHm_Chr7g0241091

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005917
Physical Location & Seq
Forward (+)
67079927 .. 67081888
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21608

Sequence Viewer

Length: 1962 bp
ATGGCAAATGCATTATTCTCGTTCACACCATTTGATTTCGAAATCCAAGGCGGTTTGAGTCCCCCCCAGCTTGAAGATTATGACAAAGAAGAAACAGTAATGTTCAATGTTGGCAAGCGAGACCATTTGTTTGGACTAGAAGATCAGTTGGGAGGAATGAATACTCTTTCTTCTGAACATGGTTTCTACCAAGACAACGATGCCAAGACAGGAGTAGTAGTATGCCATTTGTCCAAAGATCAAAACCAACAAAGTGATCTTGAAAACCAACAGCAATCCAAGACAGAATTTTTGATTCTGGATAACTTCGACTTCGAGACTGTTTCTTCTCAACATGGTTTTTATCATGCCAATAAGGCGGGGAAGGATCACCAACACCACCATTACCAACAACAGTCCAAGTCAAGTTTTTCAATTTTGGAGAATTTCAGTAACTTTGATTTTTACTTCCCCTCCGGACAAGTTACAGAACCAGTTGAGAATGATGCTCGGTTTACACACAGCCAGATCAGAGGCTCAGACATTTTCGAAATGAACACGCAATTGCTGCCTCCTCAATCTTCTCTAGGAACACTGGAGCTCCTAAACAGTTATGGAAGCGCGTTCAAGAAGTTGAAGGGAGAAAGACAAAGCAGTAGCAGGGCAGTTAAGGGTAATGAAATTGATCAAAACAGCAGGTACGTTGTTAATGGTAAACAGAAACTGTCCACCGAAGAGGTCATGAGGGTTGCTGGAGCAAGGTATGTGCAATTCTCTAGTCAGGGGTATGAAAATTTGTACATGTCTATGCACCCTTTTGGCTATGCGCTTTCAGGTCTCTGCGAAGAAGAAACAAAAGATGTAGAACTTGTTCACTTACTGTTAGCTGCAGCAGAGAAAGTAGGGTACCAACAATTTGAAAGAGCAAGCAGATTGCTTCTGCAGTGTGAATGGATTCCATCCTTCAAAGGTACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCAGAGGCACTCCGTGAGAGGATCGAAAAAGAAACAGGACAGTATACTTCTAAGGGACATGAAGAATTGTCTCAATATGCACATGGTTTGGGGACAAACCTTGCATACATCGCATGCCACCGAGAAGTCCCTTTCCATCCCGTACTGCAGTTCGCGGCAATCCAAGCCATGATAGAAAATGTTGCAAATGAGAGTAGGATCCATTTGATTGATCTGGAAATCAGGAGTGGTGTGCAATGGACAGGGTGGATGGAAGCAATTGCAGAACGAGAGGAATGCCCGGTTAAGCTTCTTACTATTACTGCTGTGGGAGTTGGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGGCAAGTGTTGCAATGTCCTTGAACCTACCCTTTAAATTTAAGGAAGTTATTGTATCAGACATGCAAGACATTAATAACCAGTTATTCGAAGACATTGAAGATGATGAAGCTGTGGTTGTGTACGCACCATTAATACTGAGGACAATGATAGCAAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATATCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCCCCATCATTTGTGCACCGTTTCATTGATACATTGTTTTTTTATAGTGCATTTTTCGACTGCCTTGAAACCTGTATGAAACAGGAGAAGTACAGGGTGATCATAGAAGGCTTGTTGCGTGAGGGAATCCGGAACATAGTGGCGGCGGAAGGGAGTGAAAGGGTTGTTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCAAGGTTTAGCATGGTGGAAATGAGCTTTAGTAACTCATCATTTTATCAAGCTAGTCTAGTGGCTAAAAAGTTCTGTTGCACCACACTAGATAGGAATGGAAAATGTCTCACTGTTGGGTGGAAGGGAACCCCAATTCATTCTCTTTCCGCTTGGAAGTTCCGTCTTGAAAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

653

Amino Acids

74.36

Weight (kDa)

5.63

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 283 - 647 1.8e-75 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 666
Acc65I GGTACC 1 cut(s) 885
AccB1I GGYRCC 1 cut(s) 885
AccI GTMKAC 1 cut(s) 1025
AccII CGCG 2 cut(s) 602, 1136
AccIII TCCGGA 2 cut(s) 455, 1707
AciI CCGC 6 cut(s) 51, 359, 1136, 1721, 1724, 1932
AclWI GGATC 4 cut(s) 375, 1010, 1174, 1187
AcsI RAATTY 4 cut(s) 287, 424, 772, 1370
AdeI CACNNNGTG 1 cut(s) 995
AfaI GTAC 7 cut(s) 680, 779, 887, 952, 1125, 1457, 1670
AflIII ACRYGT 1 cut(s) 780
AluBI AGCT 8 cut(s) 70, 580, 866, 1270, 1445, 1568, 1809, 1835
AluI AGCT 8 cut(s) 70, 580, 866, 1270, 1445, 1568, 1809, 1835
Alw21I GWGCWC 2 cut(s) 582, 1596
Alw26I GTCTC 6 cut(s) 114, 311, 821, 1056, 1313, 1895
Alw44I GTGCAC 1 cut(s) 1592
AlwI GGATC 4 cut(s) 375, 1010, 1174, 1187
AlwNI CAGNNNCTG 1 cut(s) 703
Aor13HI TCCGGA 2 cut(s) 455, 1707
AoxI GGCC 2 cut(s) 1490, 1773
ApaLI GTGCAC 1 cut(s) 1592
ApeKI GCWGC 3 cut(s) 547, 866, 869
ApoI RAATTY 4 cut(s) 287, 424, 772, 1370
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 274, 306
AseI ATTAAT 2 cut(s) 1407, 1466
Asp700I GAANNNNTTC 2 cut(s) 849, 933
Asp718I GGTACC 1 cut(s) 885
AspA2I CCTAGG 1 cut(s) 1493
AspLEI GCGC 2 cut(s) 602, 808
AspS9I GGNCC 1 cut(s) 1773
AsuC2I CCSGG 1 cut(s) 1262
AsuHPI GGTGA 3 cut(s) 362, 1531, 1687
AsuII TTCGAA 4 cut(s) 39, 528, 1422, 1747
AvrII CCTAGG 1 cut(s) 1493
BaeGI GKGCMC 1 cut(s) 1596
BaeI ACNNNNGTAYC 2 cut(s) 942, 975
BamHI GGATCC 1 cut(s) 1179
BanI GGYRCC 1 cut(s) 885
BanII GRGCYC 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 1431
Bbv12I GWGCWC 2 cut(s) 582, 1596
BbvI GCAGC 3 cut(s) 534, 853, 881
BccI CCATC 6 cut(s) 946, 1125, 1225, 1540, 1591, 1753
BcgI CGANNNNNNTGC 3 cut(s) 34, 1239, 1273
BclI TGATCA 2 cut(s) 664, 1677
BcnI CCSGG 1 cut(s) 1262
BcoDI GTCTC 6 cut(s) 114, 311, 821, 1056, 1313, 1895
BfaI CTAG 7 cut(s) 137, 566, 756, 1494, 1836, 1841, 1871
BfmI CTRYAG 3 cut(s) 867, 920, 1127
BfuAI ACCTGC 1 cut(s) 666
BglI GCCNNNNNGGC 1 cut(s) 356
BisI GCNGC 5 cut(s) 548, 867, 870, 1137, 1722
BlnI CCTAGG 1 cut(s) 1493
BlsI GCNGC 5 cut(s) 549, 868, 871, 1138, 1723
Bme1390I CCNGG 1 cut(s) 1262
BmgT120I GGNCC 1 cut(s) 1773
BmiI GGNNCC 3 cut(s) 887, 1181, 1912
BmrFI CCNGG 1 cut(s) 1262
BmsI GCATC 2 cut(s) 190, 475
BpiI GAAGAC 1 cut(s) 1431
BpmI CTGGAG 2 cut(s) 596, 753
Bpu14I TTCGAA 4 cut(s) 39, 528, 1422, 1747
BpuMI CCSGG 1 cut(s) 1262
BsaI GGTCTC 2 cut(s) 114, 821
BsaJI CCNNGG 2 cut(s) 46, 1493
BsaWI WCCGGW 2 cut(s) 455, 1707
BsaXI ACNNNNNCTCC 8 cut(s) 204, 234, 437, 467, 564, 594, 1562, 1592
Bse1I ACTGG 3 cut(s) 473, 579, 1414
Bse3DI GCAATG 2 cut(s) 1223, 1353
BseAI TCCGGA 2 cut(s) 455, 1707
BseDI CCNNGG 2 cut(s) 46, 1493
BseGI GGATG 4 cut(s) 938, 1117, 1236, 1768
BseMI GCAATG 2 cut(s) 1223, 1353
BseMII CTCAG 2 cut(s) 531, 1463
BseNI ACTGG 3 cut(s) 473, 579, 1414
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 1596
BseXI GCAGC 3 cut(s) 534, 853, 881
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 602, 1136
BshFI GGCC 2 cut(s) 1492, 1775
BshNI GGYRCC 1 cut(s) 885
BsiHKAI GWGCWC 2 cut(s) 582, 1596
BsiSI CCGG 3 cut(s) 456, 1262, 1708
BslFI GGGAC 4 cut(s) 45, 1050, 1087, 1094
BsmAI GTCTC 6 cut(s) 114, 311, 821, 1056, 1313, 1895
BsmFI GGGAC 4 cut(s) 45, 1050, 1087, 1094
BsmI GAATGC 1 cut(s) 1262
BsnI GGCC 2 cut(s) 1492, 1775
Bso31I GGTCTC 2 cut(s) 114, 821
Bsp119I TTCGAA 4 cut(s) 39, 528, 1422, 1747
Bsp1286I GDGCHC 2 cut(s) 582, 1596
Bsp13I TCCGGA 2 cut(s) 455, 1707
Bsp1407I TGTACA 1 cut(s) 777
BspACI CCGC 6 cut(s) 51, 359, 1136, 1721, 1724, 1932
BspANI GGCC 2 cut(s) 1492, 1775
BspCNI CTCAG 2 cut(s) 530, 1464
BspEI TCCGGA 2 cut(s) 455, 1707
BspFNI CGCG 2 cut(s) 602, 1136
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 3 cut(s) 887, 1181, 1912
BspMAI CTGCAG 3 cut(s) 871, 924, 1131
BspMI ACCTGC 1 cut(s) 666
BspPI GGATC 4 cut(s) 375, 1010, 1174, 1187
BspT104I TTCGAA 4 cut(s) 39, 528, 1422, 1747
BspT107I GGYRCC 1 cut(s) 885
BspTNI GGTCTC 2 cut(s) 114, 821
BsrDI GCAATG 2 cut(s) 1223, 1353
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 3 cut(s) 473, 579, 1414
BssECI CCNNGG 2 cut(s) 46, 1493
BssNAI GTATAC 1 cut(s) 1026
BssT1I CCWWGG 2 cut(s) 46, 1493
Bst1107I GTATAC 1 cut(s) 1026
Bst4CI ACNGT 9 cut(s) 97, 322, 396, 590, 705, 861, 1023, 1598, 1897
Bst6I CTCTTC 1 cut(s) 708
BstAPI GCANNNNNTGC 2 cut(s) 547, 1343
BstAUI TGTACA 1 cut(s) 777
BstBI TTCGAA 4 cut(s) 39, 528, 1422, 1747
BstC8I GCNNGC 3 cut(s) 116, 907, 1096
BstDEI CTNAG 3 cut(s) 517, 1032, 1472
BstF5I GGATG 4 cut(s) 938, 1117, 1236, 1768
BstFNI CGCG 2 cut(s) 602, 1136
BstHHI GCGC 2 cut(s) 602, 808
BstMAI GTCTC 6 cut(s) 114, 311, 821, 1056, 1313, 1895
BstMWI GCNNNNNNNGC 6 cut(s) 356, 547, 1091, 1145, 1343, 1781
BstNSI RCATGY 3 cut(s) 784, 1098, 1399
BstSCI CCNGG 1 cut(s) 1260
BstSFI CTRYAG 3 cut(s) 867, 920, 1127
BstSLI GKGCMC 1 cut(s) 1596
BstUI CGCG 2 cut(s) 602, 1136
BstV1I GCAGC 3 cut(s) 534, 853, 881
BstV2I GAAGAC 1 cut(s) 1431
BstX2I RGATCY 1 cut(s) 1179
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 1179
BstZ17I GTATAC 1 cut(s) 1026
BsuRI GGCC 2 cut(s) 1492, 1775
BtgZI GCGATG 1 cut(s) 1075
BtsCI GGATG 4 cut(s) 938, 1117, 1236, 1768
BtsI GCAGTG 1 cut(s) 929
BtsIMutI CAGTG 3 cut(s) 572, 929, 1893
BveI ACCTGC 1 cut(s) 666
Cac8I GCNNGC 3 cut(s) 116, 907, 1096
CaiI CAGNNNCTG 1 cut(s) 703
CciI TCATGA 1 cut(s) 720
CfoI GCGC 2 cut(s) 602, 808
Cfr13I GGNCC 1 cut(s) 1773
Csp6I GTAC 7 cut(s) 679, 778, 886, 951, 1124, 1456, 1669
CviQI GTAC 7 cut(s) 679, 778, 886, 951, 1124, 1456, 1669
DdeI CTNAG 3 cut(s) 517, 1032, 1472
DraI TTTAAA 1 cut(s) 1369
DraIII CACNNNGTG 1 cut(s) 995
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
EciI GGCGGA 1 cut(s) 1739
Ecl136II GAGCTC 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 46, 1493
Eco147I AGGCCT 1 cut(s) 1492
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 2 cut(s) 114, 821
Eco53kI GAGCTC 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 1773
EcoT14I CCWWGG 2 cut(s) 46, 1493
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 2 cut(s) 46, 1493
FaqI GGGAC 4 cut(s) 45, 1050, 1087, 1094
FauI CCCGC 1 cut(s) 352
FbaI TGATCA 2 cut(s) 664, 1677
FblI GTMKAC 1 cut(s) 1025
Fnu4HI GCNGC 5 cut(s) 548, 867, 870, 1137, 1722
FokI GGATG 4 cut(s) 925, 1104, 1243, 1775
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 5 cut(s) 548, 867, 870, 1137, 1722
FspBI CTAG 7 cut(s) 137, 566, 756, 1494, 1836, 1841, 1871
GlaI GCGC 2 cut(s) 601, 807
GluI GCNGC 5 cut(s) 548, 867, 870, 1137, 1722
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 596, 753
HaeIII GGCC 2 cut(s) 1492, 1775
HapII CCGG 3 cut(s) 456, 1262, 1708
HhaI GCGC 2 cut(s) 602, 808
Hin6I GCGC 2 cut(s) 600, 806
HinP1I GCGC 2 cut(s) 600, 806
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 4 cut(s) 58, 295, 934, 1704
HpaII CCGG 3 cut(s) 456, 1262, 1708
HphI GGTGA 3 cut(s) 362, 1531, 1687
Hpy166II GTNNAC 8 cut(s) 24, 495, 695, 708, 853, 1026, 1456, 1594
Hpy188I TCNGA 5 cut(s) 175, 512, 520, 963, 1393
Hpy8I GTNNAC 8 cut(s) 24, 495, 695, 708, 853, 1026, 1456, 1594
HpyAV CCTTC 8 cut(s) 358, 610, 952, 1548, 1679, 1721, 1786, 1900
HpyCH4III ACNGT 9 cut(s) 97, 322, 396, 590, 705, 861, 1023, 1598, 1897
HpyCH4IV ACGT 1 cut(s) 681
HpyF10VI GCNNNNNNNGC 6 cut(s) 356, 547, 1091, 1145, 1343, 1781
HpyF3I CTNAG 3 cut(s) 517, 1032, 1472
HpySE526I ACGT 1 cut(s) 681
HspAI GCGC 2 cut(s) 600, 806
Kpn2I TCCGGA 2 cut(s) 455, 1707
KpnI GGTACC 1 cut(s) 889
Ksp22I TGATCA 2 cut(s) 664, 1677
LmnI GCTCC 3 cut(s) 577, 585, 734
Lsp1109I GCAGC 3 cut(s) 534, 853, 881
LweI GCATC 2 cut(s) 190, 475
MaeI CTAG 7 cut(s) 137, 566, 756, 1494, 1836, 1841, 1871
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 3 cut(s) 431, 463, 1814
MfeI CAATTG 2 cut(s) 542, 1239
MflI RGATCY 1 cut(s) 1179
MhlI GDGCHC 2 cut(s) 582, 1596
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 1276
Mph1103I ATGCAT 1 cut(s) 13
MroI TCCGGA 2 cut(s) 455, 1707
MroXI GAANNNNTTC 2 cut(s) 849, 933
MseI TTAA 7 cut(s) 648, 687, 1266, 1368, 1374, 1407, 1466
MslI CAYNNNNRTG 2 cut(s) 785, 1559
MspI CCGG 3 cut(s) 456, 1262, 1708
MspR9I CCNGG 1 cut(s) 1262
MunI CAATTG 2 cut(s) 542, 1239
Mva1269I GAATGC 1 cut(s) 1262
MvnI CGCG 2 cut(s) 602, 1136
MwoI GCNNNNNNNGC 6 cut(s) 356, 547, 1091, 1145, 1343, 1781
NciI CCSGG 1 cut(s) 1262
NlaIV GGNNCC 3 cut(s) 887, 1181, 1912
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 784, 1098, 1399
NspV TTCGAA 4 cut(s) 39, 528, 1422, 1747
PaeI GCATGC 1 cut(s) 1098
PagI TCATGA 1 cut(s) 720
PceI AGGCCT 1 cut(s) 1492
PciI ACATGT 1 cut(s) 780
PctI GAATGC 1 cut(s) 1262
PdmI GAANNNNTTC 2 cut(s) 849, 933
PfeI GAWTC 3 cut(s) 295, 934, 1704
PkrI GCNGC 5 cut(s) 549, 868, 871, 1138, 1723
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PscI ACATGT 1 cut(s) 780
PshBI ATTAAT 2 cut(s) 1407, 1466
Psp124BI GAGCTC 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 887, 1181, 1912
PspPI GGNCC 1 cut(s) 1773
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 3 cut(s) 871, 924, 1131
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 1179
RsaI GTAC 7 cut(s) 680, 779, 887, 952, 1125, 1457, 1670
RsaNI GTAC 7 cut(s) 679, 778, 886, 951, 1124, 1456, 1669
RseI CAYNNNNRTG 2 cut(s) 785, 1559
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 7 cut(s) 648, 687, 1266, 1368, 1374, 1407, 1466
SatI GCNGC 5 cut(s) 548, 867, 870, 1137, 1722
Sau96I GGNCC 1 cut(s) 1773
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 1262
SduI GDGCHC 2 cut(s) 582, 1596
SfaNI GCATC 2 cut(s) 190, 475
SfcI CTRYAG 3 cut(s) 867, 920, 1127
SfuI TTCGAA 4 cut(s) 39, 528, 1422, 1747
SmiMI CAYNNNNRTG 2 cut(s) 785, 1559
SphI GCATGC 1 cut(s) 1098
SseBI AGGCCT 1 cut(s) 1492
SsiI CCGC 6 cut(s) 51, 359, 1136, 1721, 1724, 1932
SspMI CTAG 7 cut(s) 137, 566, 756, 1494, 1836, 1841, 1871
SstI GAGCTC 1 cut(s) 582
StuI AGGCCT 1 cut(s) 1492
StyD4I CCNGG 1 cut(s) 1260
StyI CCWWGG 2 cut(s) 46, 1493
TaaI ACNGT 9 cut(s) 97, 322, 396, 590, 705, 861, 1023, 1598, 1897
TaiI ACGT 1 cut(s) 684
TaqI TCGA 8 cut(s) 39, 309, 315, 528, 1005, 1422, 1635, 1747
TatI WGTACW 2 cut(s) 777, 1668
TauI GCSGC 2 cut(s) 1139, 1724
TfiI GAWTC 3 cut(s) 295, 934, 1704
Tru1I TTAA 7 cut(s) 648, 687, 1266, 1368, 1374, 1407, 1466
Tru9I TTAA 7 cut(s) 648, 687, 1266, 1368, 1374, 1407, 1466
TscAI CASTG 3 cut(s) 579, 929, 1900
TseI GCWGC 3 cut(s) 547, 866, 869
TspDTI ATGAA 9 cut(s) 173, 548, 672, 783, 1056, 1455, 1591, 1670, 1910
TspGWI ACGGA 2 cut(s) 983, 1934
TspRI CASTG 3 cut(s) 579, 929, 1900
VneI GTGCAC 1 cut(s) 1592
VspI ATTAAT 2 cut(s) 1407, 1466
XapI RAATTY 4 cut(s) 287, 424, 772, 1370
XceI RCATGY 3 cut(s) 784, 1098, 1399
XmaJI CCTAGG 1 cut(s) 1493
XmiI GTMKAC 1 cut(s) 1025
XmnI GAANNNNTTC 2 cut(s) 849, 933
XspI CTAG 7 cut(s) 137, 566, 756, 1494, 1836, 1841, 1871
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.