FvH4_5g39330

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003842
Physical Location & Seq
Reverse (-)
29058512 .. 29059804
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39330.t1

Sequence Viewer

Length: 462 bp
ATGGCGTCTGGCACAGAGAGTGATGGAGCTTCTGACACCCCAGAAATCTATAAAGACCCAGATGATGGGAGGCAGCGATTCTTGCTTGAATTGGAGTTCATCCAATGTCTCGCAAATCCCACATACATCCACTATTTGGCTCAAAATCGGTACTTTAAAGATGAAGCGTTTATTGGGTACTTGAAATATCTTCAGTATTGGCAGCGTCCTGAGTACACCAAGTTCATAATGTACCCTCATTGCTTGTTTTTCCTTGAGCAACTCCAAAATGCAAACTTTCGCAATGCGATGGCACATCCTGACAACAAGGAATTGGCACATAGGCAGCAATTCTACTCCTGGAAGAACTATAGGCACAACCGTTTGAAACACATATTACCGATGCCTCTTCCTGAATCTGAAGATGCACCGCCTGCTTCTGCTCCGCCTCTGCAATTTAATAGGAAGAGGAAGTGCGTGTAA

Protein Analysis

154

Amino Acids

18.3

Weight (kDa)

7.74

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med31 PF05669 25 - 117 5.2e-36 SOH1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 65, 136
AciI CCGC 2 cut(s) 410, 425
AcuI CTGAAG 2 cut(s) 176, 420
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 152, 179, 215, 233
AfiI CCNNNNNNNGG 2 cut(s) 65, 136
AgsI TTSAA 3 cut(s) 89, 184, 367
AjnI CCWGG 1 cut(s) 338
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
Alw26I GTCTC 1 cut(s) 113
ApeKI GCWGC 3 cut(s) 73, 202, 325
BbvI GCAGC 3 cut(s) 85, 214, 337
BccI CCATC 3 cut(s) 17, 59, 283
BciT130I CCWGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 113
BfmI CTRYAG 1 cut(s) 349
BisI GCNGC 3 cut(s) 74, 203, 326
BlsI GCNGC 3 cut(s) 75, 204, 327
Bme1390I CCNGG 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 2 cut(s) 372, 394
BpuEI CTTGAG 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 5
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 136
Bse3DI GCAATG 2 cut(s) 238, 289
BseBI CCWGG 1 cut(s) 340
BseGI GGATG 3 cut(s) 99, 126, 295
BseLI CCNNNNNNNGG 2 cut(s) 65, 136
BseMI GCAATG 2 cut(s) 238, 289
BseMII CTCAG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 85, 214, 337
BslI CCNNNNNNNGG 2 cut(s) 65, 136
BsmAI GTCTC 1 cut(s) 113
BspACI CCGC 2 cut(s) 410, 425
BspCNI CTCAG 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 238, 289
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 2 cut(s) 393, 440
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 99, 126, 295
BstMAI GTCTC 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 82, 413
BstNI CCWGG 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 338
BstSFI CTRYAG 1 cut(s) 349
BstV1I GCAGC 3 cut(s) 85, 214, 337
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 3 cut(s) 99, 126, 295
Cac8I GCNNGC 1 cut(s) 414
CseI GACGC 1 cut(s) 194
Csp6I GTAC 4 cut(s) 151, 178, 214, 232
CviJI RGCY 2 cut(s) 29, 140
CviKI_1 RGCY 2 cut(s) 29, 140
CviQI GTAC 4 cut(s) 151, 178, 214, 232
DdeI CTNAG 1 cut(s) 210
DraI TTTAAA 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 393, 440
EarI CTCTTC 2 cut(s) 393, 440
EciI GGCGGA 1 cut(s) 414
Eco57I CTGAAG 2 cut(s) 176, 420
EcoRII CCWGG 1 cut(s) 338
FaiI YATR 6 cut(s) 51, 124, 227, 321, 351, 374
Fnu4HI GCNGC 3 cut(s) 74, 203, 326
FokI GGATG 3 cut(s) 86, 113, 282
Fsp4HI GCNGC 3 cut(s) 74, 203, 326
GluI GCNGC 3 cut(s) 74, 203, 326
HgaI GACGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 5
HinfI GANTC 2 cut(s) 78, 395
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 2 cut(s) 34, 400
Hpy188III TCNNGA 3 cut(s) 209, 299, 392
Hpy8I GTNNAC 1 cut(s) 216
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 272, 407, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 413
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 26, 427
LpnPI CCDG 8 cut(s) 54, 72, 222, 312, 325, 352, 405, 426
Lsp1109I GCAGC 3 cut(s) 85, 214, 337
LweI GCATC 2 cut(s) 372, 394
MboII GAAGA 5 cut(s) 182, 355, 380, 413, 457
MluCI AATT 4 cut(s) 89, 311, 329, 434
MnlI CCTC 5 cut(s) 63, 246, 396, 438, 441
MseI TTAA 2 cut(s) 156, 438
MspR9I CCNGG 1 cut(s) 340
MvaI CCWGG 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 82, 413
PfeI GAWTC 2 cut(s) 78, 395
PflMI CCANNNNNTGG 2 cut(s) 65, 136
PfoI TCCNGGA 1 cut(s) 338
PkrI GCNGC 3 cut(s) 75, 204, 327
Psp6I CCWGG 1 cut(s) 338
PspGI CCWGG 1 cut(s) 338
PsrI GAACNNNNNNTAC 2 cut(s) 206, 238
RsaI GTAC 4 cut(s) 152, 179, 215, 233
RsaNI GTAC 4 cut(s) 151, 178, 214, 232
SaqAI TTAA 2 cut(s) 156, 438
SatI GCNGC 3 cut(s) 74, 203, 326
ScrFI CCNGG 1 cut(s) 340
SetI ASST 1 cut(s) 31
SfaNI GCATC 2 cut(s) 372, 394
SfcI CTRYAG 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 89, 311, 329, 434
SsiI CCGC 2 cut(s) 410, 425
StyD4I CCNGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 362
TasI AATT 4 cut(s) 89, 311, 329, 434
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 2 cut(s) 78, 395
Tru1I TTAA 2 cut(s) 156, 438
Tru9I TTAA 2 cut(s) 156, 438
TseI GCWGC 3 cut(s) 73, 202, 325
TspDTI ATGAA 3 cut(s) 88, 177, 214
Van91I CCANNNNNTGG 2 cut(s) 65, 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.