FvH4_6g01920

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009511
Physical Location & Seq
Reverse (-)
1085877 .. 1087637
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01920.t1

Sequence Viewer

Length: 1761 bp
ATGGCAATAGTTTCCACCGAGTTATTGCCTCATAGCTTCCACACTACCTCCCAACTCAAACCCACTTCCCATTCCCACCACCCCACTGCTTTGAGTTGCAGAGCTTCTTCTGCTTCTTCAATCTCAAATGGCAGAAACAGCTCCAGAAACCCAACAAGGGTGTCAGTTTCTGCTGAACCAAAATCAACACAGTTGCAAAACTATGACTTCAAAGACACCCACTTGATGAAAGTCCTCAACAGGTCTTGCAAAGCAGGGCAGTACAATGAAGCAATCTACTTTCTTGAGCTGATGGTCAACAAGGGCTACAAGCCTGATGTGATTCTCTGCACCAAGCTCATCAAAGGGTTCTTCAACTCGAGAAACATCGAAAAGGCTATTCGGGTTATGCAAATCTTGGAACAATATGGGGAGCCTGATTTGTTTGCATACAATGCTTTGATCAGTGGGTTCTGTAAGGCCAATAGGATCGAGTCAGCTAACAAAGTTCTTGATAGAATGAAGAGTCAGGGGTTCAAGCCTGATGTTGTTACATATAATATAATGATTGGGAGTCTTTGTAGTAGAGGGAAGCTTGGTTTAGCTTTGCAGGTTATGGATCGTTTAGTGAGAGACAATTGCAAGCCTACTGTGATTACATATACAATATTGATAGAAGCTATTATACTTGATGGTGGGATTAATGAAGCTATGAAGCTTTTGGACGAGATGTTGTCAAGAGGGCTTAAACCCGATATGTATACTTATAATGCCATTGTTAGGGGAATGTGCAGGGAAGGAATGCTGGATAGAGCTTTCGAGTTTGTTAAGTGCTTCGATGCCAAGGGCTGTGCGCCGAATGTGATTTCATACAACATTTTGTTGCGTGCACTTTTGAACCGTGGGAAATGGGAGGAAGGAGAGAATCTGGTGGCCAATATGTGTGCAAGAGGTTGTGAGCCTAATGTGGTGACTTATAGCATCTTGATTAGCACGTTGTGCCGAGATGGGAAAGTTGAGGATGGAATGAATGTGTTGAAGATCATGAAGGAAAAGGGGTTAACTCCGGATGCATATAGTTATGATCCATTGATATCTTGTTTCTGCAAAGAAGGGAGGCTGGATTTAGCAATAGAGCTTCTGGATTGTATGATATCTGATGGTTGCTTGCCAGATATTGTGAACTACAATACAGTCTTGGCAGCTTTGTGCAAGAATGGGAGCGCTGATCAGGCTTTGGAAATCTTCGAGAACTTGGGTGAAGTTGGTTGTCCTCCAAATGTGAGTTCTTACAATACAATGTTCAGTGCCTTGTGGAACTGTGGAGATAGAGTAAGAGCTTTGGGAATGGTATCAGACATGGTAAGCAAAGGAATTGAGCCTGATGAGATTACATACAATTCGCTTATATCGTGTTTGTGCAGAGATGGGATGGTAAATGAGGCCATTGGGTTGTTGGTAGATATGGAAGCTGGTGGTTTTCAGCCGACAGTTATCACTTACAATATAGTTCTTCTTGGGTTGAGCAAGGCTCGGAGGATTGTCGATGCCATTGAAGTGTTCACAGCAATGGTTGAAAAGGGTTGCCGGCCTAACGAAACCACTTACATTTTGCTGATTGAAGGAATTGGATTTGCAGGATGGCGAGCTGAAGCTATGGAGCTCGCAAAATCTGTCTATAGTTTAAGTGCTATATGTGAAGATTCATTTAAACGCTTGAGCAGGACCTTTCCCATGCTTGATGTGTACAAAGAGCTTACTCTGTCTGAGATTGAGAACTAG

Protein Analysis

587

Amino Acids

65.0

Weight (kDa)

5.89

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 96 - 151 5.5e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 138 - 185 1.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 138 - 168 2.4e-10 PPR repeat
PPR_2 PF13041 139 - 187 9.5e-19 PPR repeat family
PPR PF01535 143 - 172 1.5e-07 PPR repeat
PPR_3 PF13812 162 - 221 3.1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 171 - 201 1.7e-09 PPR repeat
PPR_2 PF13041 174 - 220 1.6e-14 PPR repeat family
TPR_24 PF23276 190 - 298 3.3e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 195 - 296 7.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 206 - 237 5.9e-07 PPR repeat
PPR_2 PF13041 225 - 258 7.5e-10 PPR repeat family
PPR_3 PF13812 232 - 291 2.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 240 - 269 3.3e-11 PPR repeat
PPR_2 PF13041 248 - 292 2.3e-10 PPR repeat family
PPR_2 PF13041 279 - 328 1.1e-16 PPR repeat family
PPR_long PF17177 301 - 418 2.5e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 303 - 362 5.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 310 - 343 4.5e-12 PPR repeat
PPR PF01535 317 - 347 3.5e-06 PPR repeat
PPR_2 PF13041 322 - 362 1.5e-08 PPR repeat family
PPR_1 PF12854 345 - 377 1.1e-10 PPR repeat
TPR_24 PF23276 351 - 443 1.7e-08 Fungal tetratrico peptide repeats
PPR PF01535 353 - 382 3.5e-06 PPR repeat
PPR_3 PF13812 373 - 430 1.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 380 - 410 1.3e-07 PPR repeat
PPR_2 PF13041 384 - 431 3.6e-13 PPR repeat family
PPR_2 PF13041 419 - 468 1.5e-13 PPR repeat family
PPR_long PF17177 441 - 534 1.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 444 - 498 2.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 450 - 483 2.2e-13 PPR repeat
PPR PF01535 457 - 486 2.3e-07 PPR repeat
PPR_2 PF13041 457 - 503 4.7e-13 PPR repeat family
PPR_3 PF13812 478 - 537 6.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 489 - 535 5.6e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
Acc36I ACCTGC 1 cut(s) 580
AccI GTMKAC 1 cut(s) 740
AccIII TCCGGA 1 cut(s) 1045
AclWI GGATC 3 cut(s) 476, 606, 1058
AcoI YGGCCR 1 cut(s) 912
AcuI CTGAAG 1 cut(s) 1650
AdeI CACNNNGTG 1 cut(s) 978
AfaI GTAC 2 cut(s) 263, 1727
AfeI AGCGCT 1 cut(s) 1204
AfiI CCNNNNNNNGG 2 cut(s) 157, 759
AgsI TTSAA 9 cut(s) 120, 211, 355, 517, 877, 1018, 1535, 1556, 1601
AjuI GAANNNNNNNTTGG 2 cut(s) 1249, 1281
Alw21I GWGCWC 2 cut(s) 871, 1644
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 867
AlwI GGATC 3 cut(s) 476, 606, 1058
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 358
Aor13HI TCCGGA 1 cut(s) 1045
Aor51HI AGCGCT 1 cut(s) 1204
AoxI GGCC 4 cut(s) 459, 912, 1422, 1568
ApaLI GTGCAC 1 cut(s) 867
ApeKI GCWGC 1 cut(s) 1181
AseI ATTAAT 1 cut(s) 681
AspLEI GCGC 2 cut(s) 835, 1205
AspS9I GGNCC 1 cut(s) 1704
AsuHPI GGTGA 2 cut(s) 961, 1250
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 1704
BaeGI GKGCMC 1 cut(s) 871
BalI TGGCCA 1 cut(s) 914
BanII GRGCYC 1 cut(s) 1644
BarI GAAGNNNNNNTAC 2 cut(s) 648, 680
Bbv12I GWGCWC 2 cut(s) 871, 1644
BbvI GCAGC 1 cut(s) 1193
BccI CCATC 8 cut(s) 286, 665, 980, 995, 1133, 1400, 1405, 1614
BclI TGATCA 2 cut(s) 441, 1207
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 1 cut(s) 1759
BfmI CTRYAG 1 cut(s) 1657
BfoI RGCGCY 1 cut(s) 1206
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 1 cut(s) 1182
BlsI GCNGC 1 cut(s) 1183
Bme18I GGWCC 1 cut(s) 1704
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 1704
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 4 cut(s) 808, 969, 1039, 1516
BplI GAGNNNNNCTC 2 cut(s) 1495, 1527
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 305, 1717
BsaJI CCNNGG 2 cut(s) 822, 880
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 544, 574, 1296, 1326
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 759
Bse118I RCCGGY 1 cut(s) 1566
Bse3DI GCAATG 1 cut(s) 1554
BseAI TCCGGA 1 cut(s) 1045
BseDI CCNNGG 2 cut(s) 822, 880
BseGI GGATG 4 cut(s) 1006, 1054, 1416, 1625
BseLI CCNNNNNNNGG 2 cut(s) 157, 759
BseMI GCAATG 1 cut(s) 1554
BseMII CTCAG 1 cut(s) 1737
BseSI GKGCMC 1 cut(s) 871
BseXI GCAGC 1 cut(s) 1193
BsgI GTGCAG 3 cut(s) 313, 790, 1420
BshFI GGCC 4 cut(s) 461, 914, 1424, 1570
BsiHKAI GWGCWC 2 cut(s) 871, 1644
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 2 cut(s) 1046, 1567
BslI CCNNNNNNNGG 2 cut(s) 157, 759
BsmAI GTCTC 1 cut(s) 606
BsmI GAATGC 1 cut(s) 786
BsnI GGCC 4 cut(s) 461, 914, 1424, 1570
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 871, 1644
Bsp13I TCCGGA 1 cut(s) 1045
Bsp1407I TGTACA 1 cut(s) 1725
Bsp143I GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
BspANI GGCC 4 cut(s) 461, 914, 1424, 1570
BspCNI CTCAG 1 cut(s) 1738
BspEI TCCGGA 1 cut(s) 1045
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 1 cut(s) 414
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 3 cut(s) 476, 606, 1058
BsrDI GCAATG 1 cut(s) 1554
BsrFI RCCGGY 1 cut(s) 1566
BsrGI TGTACA 1 cut(s) 1725
BssAI RCCGGY 1 cut(s) 1566
BssECI CCNNGG 2 cut(s) 822, 880
BssMI GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
BssNAI GTATAC 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 822
Bst1107I GTATAC 1 cut(s) 741
Bst4CI ACNGT 6 cut(s) 192, 631, 881, 1174, 1301, 1471
Bst6I CTCTTC 1 cut(s) 497
BstAPI GCANNNNNTGC 2 cut(s) 434, 978
BstAUI TGTACA 1 cut(s) 1725
BstC8I GCNNGC 6 cut(s) 623, 867, 1148, 1568, 1626, 1644
BstDEI CTNAG 1 cut(s) 1746
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 4 cut(s) 1006, 1054, 1416, 1625
BstH2I RGCGCY 1 cut(s) 1206
BstHHI GCGC 2 cut(s) 835, 1205
BstKTI GATC 6 cut(s) 444, 471, 601, 1023, 1066, 1210
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
BstMWI GCNNNNNNNGC 5 cut(s) 110, 138, 434, 978, 1211
BstSFI CTRYAG 1 cut(s) 1657
BstSLI GKGCMC 1 cut(s) 871
BstV1I GCAGC 1 cut(s) 1193
BstZ17I GTATAC 1 cut(s) 741
BsuRI GGCC 4 cut(s) 461, 914, 1424, 1570
BtgI CCRYGG 1 cut(s) 880
BtsCI GGATG 4 cut(s) 1006, 1054, 1416, 1625
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 3 cut(s) 84, 451, 1291
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 6 cut(s) 623, 867, 1148, 1568, 1626, 1644
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 2 cut(s) 835, 1205
Cfr10I RCCGGY 1 cut(s) 1566
Cfr13I GGNCC 1 cut(s) 1704
Csp6I GTAC 2 cut(s) 262, 1726
CviAII CATG 3 cut(s) 1024, 1339, 1714
CviQI GTAC 2 cut(s) 262, 1726
DdeI CTNAG 1 cut(s) 1746
DpnI GATC 6 cut(s) 443, 470, 600, 1022, 1065, 1209
DpnII GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
DraI TTTAAA 1 cut(s) 1690
DraIII CACNNNGTG 1 cut(s) 978
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Ecl136II GAGCTC 1 cut(s) 1642
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 1644
Eco32I GATATC 2 cut(s) 1074, 1134
Eco47I GGWCC 1 cut(s) 1704
Eco47III AGCGCT 1 cut(s) 1204
Eco53kI GAGCTC 1 cut(s) 1642
Eco57I CTGAAG 1 cut(s) 1650
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 1642
EcoO109I RGGNCCY 1 cut(s) 1704
EcoRV GATATC 2 cut(s) 1074, 1134
EcoT14I CCWWGG 1 cut(s) 822
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 1 cut(s) 1644
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 3 cut(s) 1027, 1342, 1717
FatI CATG 3 cut(s) 1023, 1338, 1713
FbaI TGATCA 2 cut(s) 441, 1207
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 1 cut(s) 1182
FokI GGATG 4 cut(s) 1013, 1061, 1423, 1632
FriOI GRGCYC 1 cut(s) 1644
Fsp4HI GCNGC 1 cut(s) 1182
FspBI CTAG 1 cut(s) 1759
GlaI GCGC 2 cut(s) 834, 1204
GluI GCNGC 1 cut(s) 1182
GsuI CTGGAG 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 1206
HaeIII GGCC 4 cut(s) 461, 914, 1424, 1570
HapII CCGG 2 cut(s) 1046, 1567
HhaI GCGC 2 cut(s) 835, 1205
Hin1II CATG 3 cut(s) 1027, 1342, 1717
Hin6I GCGC 2 cut(s) 833, 1203
HinP1I GCGC 2 cut(s) 833, 1203
HincII GTYRAC 2 cut(s) 298, 1041
HindII GTYRAC 2 cut(s) 298, 1041
HindIII AAGCTT 2 cut(s) 572, 695
HinfI GANTC 6 cut(s) 322, 473, 505, 553, 904, 1682
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 2 cut(s) 1046, 1567
HphI GGTGA 2 cut(s) 961, 1250
Hpy166II GTNNAC 7 cut(s) 298, 741, 869, 1041, 1162, 1542, 1726
Hpy188I TCNGA 4 cut(s) 1138, 1336, 1515, 1747
Hpy8I GTNNAC 7 cut(s) 298, 741, 869, 1041, 1162, 1542, 1726
HpyAV CCTTC 5 cut(s) 770, 890, 1021, 1085, 1595
HpyCH4III ACNGT 6 cut(s) 192, 631, 881, 1174, 1301, 1471
HpyCH4IV ACGT 1 cut(s) 974
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 138, 434, 978, 1211
HpyF3I CTNAG 1 cut(s) 1746
HpySE526I ACGT 1 cut(s) 974
Hsp92II CATG 3 cut(s) 1027, 1342, 1717
HspAI GCGC 2 cut(s) 833, 1203
Kpn2I TCCGGA 1 cut(s) 1045
KroI GCCGGC 1 cut(s) 1566
KroNI GCCGGC 1 cut(s) 1568
Ksp22I TGATCA 2 cut(s) 441, 1207
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
LmnI GCTCC 4 cut(s) 146, 412, 1200, 1639
Lsp1109I GCAGC 1 cut(s) 1193
LweI GCATC 4 cut(s) 808, 969, 1039, 1516
MaeI CTAG 1 cut(s) 1759
MaeII ACGT 1 cut(s) 974
MaeIII GTNAC 2 cut(s) 529, 949
MalI GATC 6 cut(s) 443, 470, 600, 1022, 1065, 1209
MboI GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
MboII GAAGA 8 cut(s) 99, 108, 343, 514, 1030, 1216, 1484, 1691
MfeI CAATTG 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 871, 1644
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 4 cut(s) 616, 1353, 1378, 1605
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 3 cut(s) 482, 514, 562
Mox20I TGGCCA 1 cut(s) 914
Mph1103I ATGCAT 1 cut(s) 1054
MroI TCCGGA 1 cut(s) 1045
MroNI GCCGGC 1 cut(s) 1566
MscI TGGCCA 1 cut(s) 914
MseI TTAA 6 cut(s) 681, 726, 807, 1040, 1664, 1689
MslI CAYNNNNRTG 2 cut(s) 1535, 1547
Msp20I TGGCCA 1 cut(s) 914
MspI CCGG 2 cut(s) 1046, 1567
MunI CAATTG 1 cut(s) 616
Mva1269I GAATGC 1 cut(s) 786
MwoI GCNNNNNNNGC 5 cut(s) 110, 138, 434, 978, 1211
NaeI GCCGGC 1 cut(s) 1568
NdeII GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
NgoMIV GCCGGC 1 cut(s) 1566
NlaIII CATG 3 cut(s) 1027, 1342, 1717
NlaIV GGNNCC 1 cut(s) 414
NmeAIII GCCGAG 1 cut(s) 1007
NmuCI GTSAC 1 cut(s) 949
NsiI ATGCAT 1 cut(s) 1054
PaeR7I CTCGAG 1 cut(s) 358
PagI TCATGA 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 1 cut(s) 1388
PctI GAATGC 1 cut(s) 786
PdiI GCCGGC 1 cut(s) 1568
PfeI GAWTC 3 cut(s) 322, 904, 1682
PkrI GCNGC 1 cut(s) 1183
PleI GAGTC 3 cut(s) 481, 513, 561
PpsI GAGTC 3 cut(s) 481, 513, 561
PpuMI RGGWCCY 1 cut(s) 1704
PshBI ATTAAT 1 cut(s) 681
PsiI TTATAA 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 1644
Psp5II RGGWCCY 1 cut(s) 1704
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 1704
PspPPI RGGWCCY 1 cut(s) 1704
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 2 cut(s) 263, 1727
RsaNI GTAC 2 cut(s) 262, 1726
RseI CAYNNNNRTG 2 cut(s) 1535, 1547
SacI GAGCTC 1 cut(s) 1644
SaqAI TTAA 6 cut(s) 681, 726, 807, 1040, 1664, 1689
SatI GCNGC 1 cut(s) 1182
Sau3AI GATC 6 cut(s) 441, 468, 598, 1020, 1063, 1207
Sau96I GGNCC 1 cut(s) 1704
SchI GAGTC 3 cut(s) 482, 514, 562
SduI GDGCHC 2 cut(s) 871, 1644
SfaNI GCATC 4 cut(s) 808, 969, 1039, 1516
SfcI CTRYAG 1 cut(s) 1657
Sfr274I CTCGAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 1704
SlaI CTCGAG 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 1535, 1547
SmlI CTYRAG 3 cut(s) 284, 358, 1696
SmoI CTYRAG 3 cut(s) 284, 358, 1696
Sse9I AATT 4 cut(s) 616, 1353, 1378, 1605
SspI AATATT 1 cut(s) 648
SspMI CTAG 1 cut(s) 1759
SstI GAGCTC 1 cut(s) 1644
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 6 cut(s) 192, 631, 881, 1174, 1301, 1471
TaiI ACGT 1 cut(s) 977
TaqI TCGA 7 cut(s) 359, 369, 471, 798, 816, 1227, 1524
TasI AATT 4 cut(s) 616, 1353, 1378, 1605
TatI WGTACW 2 cut(s) 261, 1725
TfiI GAWTC 3 cut(s) 322, 904, 1682
Tru1I TTAA 6 cut(s) 681, 726, 807, 1040, 1664, 1689
Tru9I TTAA 6 cut(s) 681, 726, 807, 1040, 1664, 1689
TscAI CASTG 3 cut(s) 91, 451, 1291
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 1 cut(s) 1181
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 9 cut(s) 242, 282, 515, 699, 707, 837, 1022, 1040, 1674
TspRI CASTG 3 cut(s) 91, 451, 1291
VneI GTGCAC 1 cut(s) 867
VpaK11BI GGWCC 1 cut(s) 1704
VspI ATTAAT 1 cut(s) 681
XcmI CCANNNNNNNNNTGG 1 cut(s) 1432
XhoI CTCGAG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 740
XspI CTAG 1 cut(s) 1759
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.