MD12G1247200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009511
Physical Location & Seq
Reverse (-)
31735288 .. 31736824
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1247200.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAAAGTTCTCGACAGGTCCTGCAAAGCAGGACAGTACAATGAAGCACTCTACTTTCTTGAGCTAATGGTTAACGAGGGGTACAGGCCTGATGTGATTCTCTACACAAAGCTCATCAAAGGGTTCTTCAATTCGAGGAACGTCGAAAAGGCAGTTCGGGTTATGCAGATTTTGGAGAACGGGTTCTGTAAGGCGAATCGGATTGATTCAGCAAACAAGGTACTTGATAGGATGAGGAGTCGGGGATTTTCACCTGATGTTGTTACTTACAATATCATGATTGGGAGTCTTTGTGGCAGGGGCAAGCTAGGATTAGCTTTGAAGGTTTTGGATCAGTTGGTGAAGGATAATTGCAGGCCGACTGTGATTACTTACATGATTTTGATCGAAGCAATGATTCTTGAAGGCGGAATTGATGAGGCGGTGAATCTTTTGGATGACATGTTGTCTGGAGGGCTTAAACCTGACATGAATACTTACAATGCAGTTGTTAAGGGAATGTGTAGAGAAGGGATGCTGGATAGGGCGTTCGGCTTAGTTAGGAGCTTAGATTCAAAGGGTTGTTCGCCTAATGTAGTCTCGTACAACATTTTGTTACGAGCGCTTTTGAATAGGGGTAAATGGGAGGAAGGGGAGAAGCTAGTGAGCAATATGTATTCGAGAGGTTGTGAACCGAATGTGGTGACTTACAGCATTTTGATTAGCACGCTTTGTCGCCATGGGAAAGTTGAGGATGCTGTGAATGTGCTGAAGATCATGAAGGACAAGGGATTGACGCCGGATGCGTATAGTTATGATCCGCTGGTTTCTGCATTCTGCAAAGAGGGGAGATTGGATTTGGCGATAGAGTTTTTGGATTACATGATATCGGATGGCTGCTTGCCGGATATTGTGAACTACAATACAATCTTGGCTGCTTTGTGTAAGAGTGGAAAAGCTGATCAGGCTTTGGAAATCTTCGAGAACCCCGGTGAAGTGGGATGTCCTCCGAATGTGAGCTCTTACAACACGATGTTCAGTGCACTGTGGAATTGCGGAGATAGAGTGAGAGCGCTTCAAATGGTGTCGGAGATGGTAAGCAAAGGAATTGACCCCGATGAGATTACTTACAATTCGCTTATATCGTGTTTGTGCAGAGACAGGATGGTGAATGAGGCTATAGGGTTGTTGGTAGACATGGAAAGTGGTGGCTTTCAGCCAACAGTTATCAGTTACAACATTGTTGTTCTCGGGCTGTGCAAAACGCGTAGGATTGTCGATGCCATTGAAGTGCTTGCGGCAATGGTTGAAAAGGGGTGCCGGCCTAATGAAACTACCTACACTTTGTTGATTGAAGGAATTGGATTCGCAGGATGGCGAGCTGAAGCGATGGAGCTTGCCAACTCTGTTTTTAGTTTAAGGGCGATTTCCGAAGATTCGTTCGGGCGTTTGAATAGAACCTTTCCCATGCTTGATGTTTACAAAGAGCTTACTCTATCTGAGATTAAGAACTAG

Protein Analysis

498

Amino Acids

55.22

Weight (kDa)

5.33

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 60 - 98 1.2e-12 PPR repeat family
PPR_3 PF13812 73 - 131 2.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 81 - 112 3.2e-09 PPR repeat
PPR_2 PF13041 85 - 132 7.1e-14 PPR repeat family
TPR_24 PF23276 101 - 209 6.3e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 120 - 169 3.1e-12 PPR repeat family
PPR_3 PF13812 144 - 202 1.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 152 - 181 2.2e-09 PPR repeat
PPR_2 PF13041 155 - 203 4.9e-13 PPR repeat family
PPR_2 PF13041 190 - 239 2.8e-18 PPR repeat family
PPR_long PF17177 211 - 322 8.7e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 213 - 273 2.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 221 - 254 6.3e-14 PPR repeat
PPR PF01535 228 - 258 3.1e-07 PPR repeat
PPR_2 PF13041 232 - 274 1.1e-10 PPR repeat family
TPR_24 PF23276 235 - 354 1.9e-12 Fungal tetratrico peptide repeats
PPR_3 PF13812 249 - 306 1.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 256 - 288 6.4e-10 PPR repeat
PPR_3 PF13812 286 - 341 3.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 291 - 321 2.7e-08 PPR repeat
PPR_2 PF13041 295 - 342 2.3e-12 PPR repeat family
PPR_2 PF13041 330 - 379 4.1e-14 PPR repeat family
PPR_long PF17177 350 - 445 3.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 361 - 394 1.9e-11 PPR repeat
PPR_2 PF13041 365 - 414 2.4e-14 PPR repeat family
PPR PF01535 368 - 398 6.6e-07 PPR repeat
PPR_3 PF13812 389 - 448 6.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 400 - 446 1.1e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1296
AccI GTMKAC 1 cut(s) 1173
AccII CGCG 1 cut(s) 1246
AciI CCGC 5 cut(s) 408, 422, 800, 1035, 1277
AclWI GGATC 2 cut(s) 339, 791
AcuI CTGAAG 2 cut(s) 770, 1383
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 4 cut(s) 38, 83, 222, 584
AfeI AGCGCT 2 cut(s) 603, 1053
AflIII ACRYGT 2 cut(s) 441, 1244
AhdI GACNNNNNGTC 1 cut(s) 445
AloI GAACNNNNNNTCC 4 cut(s) 167, 199, 512, 544
Alw21I GWGCWC 2 cut(s) 1001, 1024
Alw26I GTCTC 2 cut(s) 583, 1131
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 2 cut(s) 339, 791
AlwNI CAGNNNCTG 1 cut(s) 21
Ama87I CYCGRG 1 cut(s) 1229
Aor51HI AGCGCT 2 cut(s) 603, 1053
AoxI GGCC 3 cut(s) 86, 356, 1301
ApaLI GTGCAC 1 cut(s) 1020
ApeKI GCWGC 2 cut(s) 876, 914
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 2 cut(s) 604, 1054
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 969
AsuHPI GGTGA 6 cut(s) 243, 352, 436, 694, 983, 1159
AvaI CYCGRG 1 cut(s) 1229
AvaII GGWCC 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 1024
BanI GGYRCC 1 cut(s) 1296
BanII GRGCYC 1 cut(s) 1001
Bbv12I GWGCWC 2 cut(s) 1001, 1024
BbvI GCAGC 2 cut(s) 863, 901
BccI CCATC 5 cut(s) 866, 1066, 1138, 1347, 1363
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 1 cut(s) 969
BcoDI GTCTC 2 cut(s) 583, 1131
BfaI CTAG 3 cut(s) 308, 641, 1492
BfmI CTRYAG 1 cut(s) 1158
BfoI RGCGCY 2 cut(s) 605, 1055
BisI GCNGC 3 cut(s) 877, 915, 1278
BlsI GCNGC 3 cut(s) 878, 916, 1279
Bme1390I CCNGG 1 cut(s) 969
Bme18I GGWCC 1 cut(s) 18
BmeRI GACNNNNNGTC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 1229
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 1 cut(s) 1298
BmrFI CCNGG 1 cut(s) 969
BmsI GCATC 4 cut(s) 504, 724, 772, 1249
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 969
BsaBI GATNNNNATC 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 776
BsaJI CCNNGG 2 cut(s) 718, 967
BsaXI ACNNNNNCTCC 6 cut(s) 167, 197, 277, 307, 1029, 1059
Bse118I RCCGGY 1 cut(s) 1299
Bse3DI GCAATG 2 cut(s) 399, 1287
Bse8I GATNNNNATC 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 718, 967
BseGI GGATG 9 cut(s) 237, 442, 519, 739, 787, 877, 986, 1149, 1358
BseJI GATNNNNATC 1 cut(s) 383
BseMI GCAATG 2 cut(s) 399, 1287
BseMII CTCAG 1 cut(s) 1470
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 1024
BseXI GCAGC 2 cut(s) 863, 901
BsgI GTGCAG 1 cut(s) 1153
Bsh1236I CGCG 1 cut(s) 1246
BshFI GGCC 3 cut(s) 88, 358, 1303
BshNI GGYRCC 1 cut(s) 1296
BsiHKAI GWGCWC 2 cut(s) 1001, 1024
BsiHKCI CYCGRG 1 cut(s) 1229
BsiSI CCGG 4 cut(s) 779, 884, 969, 1300
BsmAI GTCTC 2 cut(s) 583, 1131
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 3 cut(s) 88, 358, 1303
BsoBI CYCGRG 1 cut(s) 1229
Bsp1286I GDGCHC 2 cut(s) 1001, 1024
Bsp143I GATC 5 cut(s) 331, 384, 753, 796, 940
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 5 cut(s) 408, 422, 800, 1035, 1277
BspANI GGCC 3 cut(s) 88, 358, 1303
BspCNI CTCAG 1 cut(s) 1471
BspFNI CGCG 1 cut(s) 1246
BspHI TCATGA 2 cut(s) 276, 756
BspLI GGNNCC 1 cut(s) 1298
BspPI GGATC 2 cut(s) 339, 791
BspT107I GGYRCC 1 cut(s) 1296
BsrDI GCAATG 2 cut(s) 399, 1287
BsrFI RCCGGY 1 cut(s) 1299
BssAI RCCGGY 1 cut(s) 1299
BssECI CCNNGG 2 cut(s) 718, 967
BssMI GATC 5 cut(s) 331, 384, 753, 796, 940
BssNI GRCGYC 1 cut(s) 776
BssT1I CCWWGG 1 cut(s) 718
Bst4CI ACNGT 4 cut(s) 36, 364, 1026, 1204
BstACI GRCGYC 1 cut(s) 776
BstC8I GCNNGC 8 cut(s) 305, 356, 707, 881, 1275, 1301, 1359, 1377
BstDEI CTNAG 3 cut(s) 535, 547, 1479
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 9 cut(s) 237, 442, 519, 739, 787, 877, 986, 1149, 1358
BstFNI CGCG 1 cut(s) 1246
BstH2I RGCGCY 2 cut(s) 605, 1055
BstHHI GCGC 2 cut(s) 604, 1054
BstKTI GATC 5 cut(s) 334, 387, 756, 799, 943
BstMAI GTCTC 2 cut(s) 583, 1131
BstMBI GATC 5 cut(s) 331, 384, 753, 796, 940
BstMWI GCNNNNNNNGC 1 cut(s) 944
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 967
BstSFI CTRYAG 1 cut(s) 1158
BstSLI GKGCMC 1 cut(s) 1024
BstUI CGCG 1 cut(s) 1246
BstV1I GCAGC 2 cut(s) 863, 901
BsuRI GGCC 3 cut(s) 88, 358, 1303
BtgI CCRYGG 1 cut(s) 718
BtgZI GCGATG 1 cut(s) 1382
BtsCI GGATG 9 cut(s) 237, 442, 519, 739, 787, 877, 986, 1149, 1358
BtsIMutI CAGTG 2 cut(s) 1022, 1024
Cac8I GCNNGC 8 cut(s) 305, 356, 707, 881, 1275, 1301, 1359, 1377
CaiI CAGNNNCTG 1 cut(s) 21
CciI TCATGA 2 cut(s) 276, 756
CfoI GCGC 2 cut(s) 604, 1054
Cfr10I RCCGGY 1 cut(s) 1299
Cfr13I GGNCC 1 cut(s) 18
CseI GACGC 1 cut(s) 784
Csp6I GTAC 4 cut(s) 37, 82, 221, 583
CviAII CATG 9 cut(s) 277, 376, 442, 469, 719, 757, 862, 1177, 1447
CviQI GTAC 4 cut(s) 37, 82, 221, 583
DdeI CTNAG 3 cut(s) 535, 547, 1479
DpnI GATC 5 cut(s) 333, 386, 755, 798, 942
DpnII GATC 5 cut(s) 331, 384, 753, 796, 940
DriI GACNNNNNGTC 1 cut(s) 445
Eam1105I GACNNNNNGTC 1 cut(s) 445
EciI GGCGGA 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 999
Eco130I CCWWGG 1 cut(s) 718
Eco147I AGGCCT 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 1001
Eco32I GATATC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 18
Eco47III AGCGCT 2 cut(s) 603, 1053
Eco53kI GAGCTC 1 cut(s) 999
Eco57I CTGAAG 2 cut(s) 770, 1383
Eco88I CYCGRG 1 cut(s) 1229
EcoICRI GAGCTC 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 18
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 1001
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 9 cut(s) 280, 379, 445, 472, 722, 760, 865, 1180, 1450
FatI CATG 9 cut(s) 276, 375, 441, 468, 718, 756, 861, 1176, 1446
FbaI TGATCA 1 cut(s) 940
FblI GTMKAC 1 cut(s) 1173
Fnu4HI GCNGC 3 cut(s) 877, 915, 1278
FokI GGATG 9 cut(s) 244, 449, 526, 746, 794, 884, 993, 1156, 1365
FriOI GRGCYC 1 cut(s) 1001
Fsp4HI GCNGC 3 cut(s) 877, 915, 1278
FspBI CTAG 3 cut(s) 308, 641, 1492
GlaI GCGC 2 cut(s) 603, 1053
GluI GCNGC 3 cut(s) 877, 915, 1278
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 2 cut(s) 605, 1055
HaeIII GGCC 3 cut(s) 88, 358, 1303
HapII CCGG 4 cut(s) 779, 884, 969, 1300
HgaI GACGC 1 cut(s) 784
HhaI GCGC 2 cut(s) 604, 1054
Hin1I GRCGYC 1 cut(s) 776
Hin1II CATG 9 cut(s) 280, 379, 445, 472, 722, 760, 865, 1180, 1450
Hin6I GCGC 2 cut(s) 602, 1052
HinP1I GCGC 2 cut(s) 602, 1052
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 4 cut(s) 779, 884, 969, 1300
HphI GGTGA 6 cut(s) 243, 352, 436, 694, 983, 1159
Hpy166II GTNNAC 6 cut(s) 73, 671, 895, 1022, 1174, 1459
Hpy188I TCNGA 6 cut(s) 201, 871, 990, 1069, 1411, 1480
Hpy188III TCNNGA 8 cut(s) 11, 59, 277, 401, 450, 660, 757, 961
Hpy8I GTNNAC 6 cut(s) 73, 671, 895, 1022, 1174, 1459
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 7 cut(s) 316, 337, 398, 503, 623, 754, 1328
HpyCH4III ACNGT 4 cut(s) 36, 364, 1026, 1204
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 9 cut(s) 24, 166, 354, 485, 812, 819, 1022, 1134, 1239
HpyF10VI GCNNNNNNNGC 1 cut(s) 944
HpyF3I CTNAG 3 cut(s) 535, 547, 1479
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 776
Hsp92II CATG 9 cut(s) 280, 379, 445, 472, 722, 760, 865, 1180, 1450
HspAI GCGC 2 cut(s) 602, 1052
KroI GCCGGC 1 cut(s) 1299
KroNI GCCGGC 1 cut(s) 1301
Ksp22I TGATCA 1 cut(s) 940
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 5 cut(s) 331, 384, 753, 796, 940
LmnI GCTCC 2 cut(s) 543, 1372
Lsp1109I GCAGC 2 cut(s) 863, 901
LweI GCATC 4 cut(s) 504, 724, 772, 1249
MaeI CTAG 3 cut(s) 308, 641, 1492
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 4 cut(s) 262, 594, 682, 1211
MalI GATC 5 cut(s) 333, 386, 755, 798, 942
MboI GATC 5 cut(s) 331, 384, 753, 796, 940
MboII GAAGA 4 cut(s) 118, 763, 949, 1424
MhlI GDGCHC 2 cut(s) 1001, 1024
MluCI AATT 7 cut(s) 130, 349, 411, 1030, 1086, 1111, 1338
MluI ACGCGT 1 cut(s) 1244
MlyI GAGTC 2 cut(s) 247, 295
MmeI TCCRAC 1 cut(s) 1047
MroNI GCCGGC 1 cut(s) 1299
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 5 cut(s) 72, 459, 492, 1397, 1485
MslI CAYNNNNRTG 1 cut(s) 1268
MspA1I CMGCKG 1 cut(s) 802
MspI CCGG 4 cut(s) 779, 884, 969, 1300
MspR9I CCNGG 1 cut(s) 969
Mva1269I GAATGC 1 cut(s) 812
MvnI CGCG 1 cut(s) 1246
MwoI GCNNNNNNNGC 1 cut(s) 944
NaeI GCCGGC 1 cut(s) 1301
NciI CCSGG 1 cut(s) 969
NcoI CCATGG 1 cut(s) 718
NdeII GATC 5 cut(s) 331, 384, 753, 796, 940
NgoMIV GCCGGC 1 cut(s) 1299
NlaIII CATG 9 cut(s) 280, 379, 445, 472, 722, 760, 865, 1180, 1450
NlaIV GGNNCC 1 cut(s) 1298
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 1 cut(s) 445
PagI TCATGA 2 cut(s) 276, 756
PceI AGGCCT 1 cut(s) 88
PciI ACATGT 1 cut(s) 441
PcsI WCGNNNNNNNCGW 2 cut(s) 782, 1121
PctI GAATGC 1 cut(s) 812
PdiI GCCGGC 1 cut(s) 1301
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 8 cut(s) 97, 196, 206, 397, 427, 551, 1344, 1415
PkrI GCNGC 3 cut(s) 878, 916, 1279
PleI GAGTC 2 cut(s) 246, 294
PpsI GAGTC 2 cut(s) 246, 294
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 1001
Psp5II RGGWCCY 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 1298
PspPI GGNCC 1 cut(s) 18
PspPPI RGGWCCY 1 cut(s) 18
PstNI CAGNNNCTG 1 cut(s) 21
RsaI GTAC 4 cut(s) 38, 83, 222, 584
RsaNI GTAC 4 cut(s) 37, 82, 221, 583
RseI CAYNNNNRTG 1 cut(s) 1268
SacI GAGCTC 1 cut(s) 1001
SaqAI TTAA 5 cut(s) 72, 459, 492, 1397, 1485
SatI GCNGC 3 cut(s) 877, 915, 1278
Sau3AI GATC 5 cut(s) 331, 384, 753, 796, 940
Sau96I GGNCC 1 cut(s) 18
SchI GAGTC 2 cut(s) 247, 295
ScrFI CCNGG 1 cut(s) 969
SduI GDGCHC 2 cut(s) 1001, 1024
SfaNI GCATC 4 cut(s) 504, 724, 772, 1249
SfcI CTRYAG 1 cut(s) 1158
SinI GGWCC 1 cut(s) 18
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 7 cut(s) 130, 349, 411, 1030, 1086, 1111, 1338
SseBI AGGCCT 1 cut(s) 88
SsiI CCGC 5 cut(s) 408, 422, 800, 1035, 1277
SspMI CTAG 3 cut(s) 308, 641, 1492
SstI GAGCTC 1 cut(s) 1001
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 967
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 4 cut(s) 36, 364, 1026, 1204
TaiI ACGT 1 cut(s) 144
TaqI TCGA 7 cut(s) 12, 134, 144, 387, 659, 960, 1257
TasI AATT 7 cut(s) 130, 349, 411, 1030, 1086, 1111, 1338
TatI WGTACW 1 cut(s) 36
TauI GCSGC 1 cut(s) 1280
TfiI GAWTC 8 cut(s) 97, 196, 206, 397, 427, 551, 1344, 1415
Tru1I TTAA 5 cut(s) 72, 459, 492, 1397, 1485
Tru9I TTAA 5 cut(s) 72, 459, 492, 1397, 1485
TscAI CASTG 2 cut(s) 1024, 1029
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 2 cut(s) 876, 914
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 5 cut(s) 17, 57, 485, 773, 1323
TspRI CASTG 2 cut(s) 1024, 1029
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 1 cut(s) 18
XceI RCATGY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 1173
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 3 cut(s) 308, 641, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.