RchiOBHm_Chr3g0449651

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009511
Physical Location & Seq
Reverse (-)
1355283 .. 1357424
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41701

Sequence Viewer

Length: 1770 bp
ATGGCAATACTCTCCACTGAGTTATTGCCTCATAGCTTCCACACTACCTCCCAACTCAAACCCACTTCCCATTCCCACCACCCCACTGCTTTGAGTTGTAGACCTTCTTCTTCTTCCTCCTCTTCAATCTCAAATGGCAGAAACTACACCTCCAGAAACCTAACAAGGGTGTCTGTCTCTGCTGAACCAAAACCAACACACTTGCAAAGCCATGATTACAAAGAGACCCACTTGATCAAAGTCCTCAACAGGTCTTGCAAAGCAGGCCAGTACAATGAAGCCGTCTACTTTCTTGAGCTCATGGTCAACAAGGGCTACAAGCCTGATGTGATTCTCTGCACCAAGCTCATCAAAGGCTTCTTCAACTCGAGAAACATCGAAAAGGCCGTTCGGGTTATGCAAATATTGGAACAGTATGGGGAGCCTGATTTGTTTTCATACAATGCTTTGATCAGTGGGTTCTATAAGGCCAATAGGATTGAATCAGCAAACAAAGTTCTTGATAGAATGAAGAGTCAGGGGTTCAAGCCTGATGTTGTTACATATAATATAATGATTGGGAGTCTCTGTAGTAGAGGGAAGCTTGGGTTAGCTAGGAAGGTCATGGATCGTTTAGTGAAAGACAATTGCAACCCTACTGTGATTACATATACAATATTGATAGAAGCTACTATTCTTGATGGTGGGATTGATGAAGCTATGAAGCTTTTGGATGAGATGTTGTCAAGAGGGCTTAGACCTGATATGTATACTTACAATGCCATTGTTAGGGGAATGTGCAGGGAAGGAATGCTGGATAGAGCTTTCGAGTTTGTTCGGAGCTTCGATGCTAAAGGCTGTGCGCCGAATGTGATCTCATACAACATATTGTTGCGTGCGCTTTTGAACCGTGGGAAATGGGAGGAAGGTGAGAATCTAGTGAGCAATATGTGTGCAAGAGGTTGTGAGCCTAATGTGGTGACTTATAGCATCTTGATTAGCACGCTGTGCCGCGATGGGAAAGTTGAGGATGCAGTGAATGTGTTGAAGATTATGAAGGAAAAGGGGTTAACTCCGGATGCATATAGTTATGATCCATTGATTTCAACTTTCTGCAAAGAAGGGAGGTTAGATTTAGCAATAGAGTTTTTGGATTGTATGATATCTGATGGTTGCTTGCCAGATATTGTGAACTACAATACAATCTTGGCTGCTTTGTGCAAGAATGGGAGTGCTGATCAGGCTTTGCAAATCTTCGAGAACTTGGGTGAAGTCGGTTGTCCTCCAAATGTGAGTTCCTACAACACAATGTTCAGTGCATTGTGGAATTGTGGAGATAGAGTAAGAGCTTTGGGAATGGTATCAGACATGGTAAGCAAAGGAATTGAGCCTGATGAGATCACATACAATTCGCTTATATCGTGTCTGTGCAGAGATGGGATGGTAAATGAGGCAATTGGGTTGTTGGTAGACATGGAAACTGGTGGTTTTCAGCCTACAGTTATCACTTACAATATTGTTCTTCTTGGGTTGAGCAAGGCTCGGAGGATTATCGATGCCATTGAAGTGTTCACAGCAATGGTTGAAAAGGGTTGCCGGCCTAACGAAACTACTTACATTTTGCTGATTGAAGGAATTGGATTTGCAGGATGGCGAGCTGAAGCTATGGAGCTTGCAAAATCTGTTTATAGTTTAAGGGCTATTTCCGAAGATTCTTTCAAACGCTTGAACAGGACCTTTCCCATGCTTGATGTTTACAAAGAGCTTACTCTGTCTGAGATTAAGAACTAG

Protein Analysis

589

Amino Acids

65.64

Weight (kDa)

6.73

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 99 - 152 2.9e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 141 - 188 2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 141 - 171 5e-07 PPR repeat
PPR_2 PF13041 142 - 190 2.1e-17 PPR repeat family
PPR PF01535 145 - 175 2.3e-06 PPR repeat
PPR_3 PF13812 165 - 223 1.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 174 - 204 3.9e-09 PPR repeat
PPR_2 PF13041 177 - 223 4.8e-14 PPR repeat family
TPR_24 PF23276 193 - 301 7.6e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 198 - 299 4.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 209 - 240 4.7e-07 PPR repeat
PPR_2 PF13041 228 - 261 1.7e-09 PPR repeat family
PPR_3 PF13812 235 - 294 4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 243 - 273 7.7e-11 PPR repeat
PPR_2 PF13041 251 - 295 9.5e-11 PPR repeat family
PPR_2 PF13041 282 - 331 5.9e-17 PPR repeat family
PPR_long PF17177 304 - 420 3.4e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 306 - 366 2.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 313 - 346 7.2e-14 PPR repeat
PPR PF01535 320 - 350 4.9e-07 PPR repeat
PPR_2 PF13041 324 - 366 1.6e-10 PPR repeat family
PPR_1 PF12854 348 - 380 9.4e-10 PPR repeat
TPR_24 PF23276 354 - 446 2.3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 380 - 433 1.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 383 - 413 5.4e-08 PPR repeat
PPR_2 PF13041 387 - 434 2.2e-13 PPR repeat family
PPR_2 PF13041 422 - 471 1.5e-13 PPR repeat family
PPR_long PF17177 444 - 537 1.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 447 - 501 2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 453 - 486 2.3e-13 PPR repeat
PPR_2 PF13041 457 - 506 1.8e-14 PPR repeat family
PPR PF01535 460 - 489 2e-07 PPR repeat
PPR_3 PF13812 481 - 540 1.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 492 - 538 2e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 100, 285, 749, 1449
AccII CGCG 1 cut(s) 993
AccIII TCCGGA 1 cut(s) 1054
AciI CCGC 1 cut(s) 991
AclWI GGATC 2 cut(s) 615, 1067
AcuI CTGAAG 1 cut(s) 1659
AdeI CACNNNGTG 1 cut(s) 987
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 166, 768
AjuI GAANNNNNNNTTGG 2 cut(s) 1258, 1290
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 3 cut(s) 181, 218, 569
AlwI GGATC 2 cut(s) 615, 1067
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 1054
AoxI GGCC 4 cut(s) 265, 384, 468, 1577
ApeKI GCWGC 1 cut(s) 1190
AspLEI GCGC 2 cut(s) 844, 880
AspS9I GGNCC 1 cut(s) 1713
AsuHPI GGTGA 3 cut(s) 920, 970, 1259
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 1 cut(s) 1713
BanII GRGCYC 1 cut(s) 300
BarI GAAGNNNNNNTAC 2 cut(s) 91, 123
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 1177
BccI CCATC 6 cut(s) 674, 989, 1142, 1409, 1414, 1623
BceAI ACGGC 2 cut(s) 266, 371
BclI TGATCA 3 cut(s) 234, 450, 1216
BcoDI GTCTC 3 cut(s) 181, 218, 569
BfaI CTAG 3 cut(s) 594, 917, 1768
BfmI CTRYAG 2 cut(s) 568, 1476
BisI GCNGC 2 cut(s) 991, 1191
BlsI GCNGC 2 cut(s) 992, 1192
Bme18I GGWCC 1 cut(s) 1713
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 1713
BmiI GGNNCC 1 cut(s) 423
BmsI GCATC 5 cut(s) 817, 978, 1000, 1048, 1525
BplI GAGNNNNNCTC 2 cut(s) 1504, 1536
BpmI CTGGAG 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 314
Bsa29I ATCGAT 1 cut(s) 1533
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 889
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 134, 164, 553, 583, 1305, 1335
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 768
Bse118I RCCGGY 1 cut(s) 1575
Bse1I ACTGG 2 cut(s) 268, 1465
Bse3DI GCAATG 1 cut(s) 1563
BseAI TCCGGA 1 cut(s) 1054
BseCI ATCGAT 1 cut(s) 1533
BseDI CCNNGG 1 cut(s) 889
BseGI GGATG 5 cut(s) 718, 1015, 1063, 1425, 1634
BseLI CCNNNNNNNGG 2 cut(s) 166, 768
BseMI GCAATG 1 cut(s) 1563
BseMII CTCAG 2 cut(s) 9, 1746
BseNI ACTGG 2 cut(s) 268, 1465
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 1177
BsgI GTGCAG 3 cut(s) 322, 799, 1429
Bsh1236I CGCG 1 cut(s) 993
BshFI GGCC 4 cut(s) 267, 386, 470, 1579
BshVI ATCGAT 1 cut(s) 1533
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 2 cut(s) 1055, 1576
BslI CCNNNNNNNGG 2 cut(s) 166, 768
BsmAI GTCTC 3 cut(s) 181, 218, 569
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 4 cut(s) 267, 386, 470, 1579
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 300
Bsp13I TCCGGA 1 cut(s) 1054
Bsp143I GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
BspACI CCGC 1 cut(s) 991
BspANI GGCC 4 cut(s) 267, 386, 470, 1579
BspCNI CTCAG 2 cut(s) 10, 1747
BspDI ATCGAT 1 cut(s) 1533
BspEI TCCGGA 1 cut(s) 1054
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 2 cut(s) 615, 1067
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 1563
BsrFI RCCGGY 1 cut(s) 1575
BsrI ACTGG 2 cut(s) 268, 1465
BssAI RCCGGY 1 cut(s) 1575
BssECI CCNNGG 1 cut(s) 889
BssMI GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
BssNAI GTATAC 1 cut(s) 750
Bst1107I GTATAC 1 cut(s) 750
Bst4CI ACNGT 4 cut(s) 414, 640, 890, 1480
Bst6I CTCTTC 2 cut(s) 127, 506
BstAPI GCANNNNNTGC 1 cut(s) 987
BstC8I GCNNGC 7 cut(s) 265, 876, 983, 1157, 1577, 1635, 1653
BstDEI CTNAG 3 cut(s) 18, 734, 1755
BstDSI CCRYGG 1 cut(s) 889
BstF5I GGATG 5 cut(s) 718, 1015, 1063, 1425, 1634
BstFNI CGCG 1 cut(s) 993
BstHHI GCGC 2 cut(s) 844, 880
BstKTI GATC 7 cut(s) 237, 453, 610, 855, 1075, 1219, 1380
BstMAI GTCTC 3 cut(s) 181, 218, 569
BstMBI GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
BstMWI GCNNNNNNNGC 3 cut(s) 264, 987, 1220
BstSFI CTRYAG 2 cut(s) 568, 1476
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 1 cut(s) 1177
BstZ17I GTATAC 1 cut(s) 750
Bsu15I ATCGAT 1 cut(s) 1533
BsuRI GGCC 4 cut(s) 267, 386, 470, 1579
BsuTUI ATCGAT 1 cut(s) 1533
BtgI CCRYGG 1 cut(s) 889
BtgZI GCGATG 1 cut(s) 1008
BtsCI GGATG 5 cut(s) 718, 1015, 1063, 1425, 1634
BtsI GCAGTG 2 cut(s) 84, 1020
BtsIMutI CAGTG 5 cut(s) 15, 84, 460, 1020, 1300
Cac8I GCNNGC 7 cut(s) 265, 876, 983, 1157, 1577, 1635, 1653
CfoI GCGC 2 cut(s) 844, 880
Cfr10I RCCGGY 1 cut(s) 1575
Cfr13I GGNCC 1 cut(s) 1713
ClaI ATCGAT 1 cut(s) 1533
Csp6I GTAC 1 cut(s) 271
CviAII CATG 6 cut(s) 212, 301, 604, 1348, 1453, 1723
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 3 cut(s) 18, 734, 1755
DpnI GATC 7 cut(s) 236, 452, 609, 854, 1074, 1218, 1379
DpnII GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
DraIII CACNNNGTG 1 cut(s) 987
Eam1104I CTCTTC 2 cut(s) 127, 506
EarI CTCTTC 2 cut(s) 127, 506
Ecl136II GAGCTC 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 1143
Eco47I GGWCC 1 cut(s) 1713
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 1659
Eco88I CYCGRG 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 1713
EcoRV GATATC 1 cut(s) 1143
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 1 cut(s) 300
FaeI CATG 6 cut(s) 215, 304, 607, 1351, 1456, 1726
FatI CATG 6 cut(s) 211, 300, 603, 1347, 1452, 1722
FbaI TGATCA 3 cut(s) 234, 450, 1216
FblI GTMKAC 4 cut(s) 100, 285, 749, 1449
Fnu4HI GCNGC 2 cut(s) 991, 1191
FokI GGATG 5 cut(s) 725, 1022, 1070, 1432, 1641
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 2 cut(s) 991, 1191
FspBI CTAG 3 cut(s) 594, 917, 1768
GlaI GCGC 2 cut(s) 843, 879
GluI GCNGC 2 cut(s) 991, 1191
GsuI CTGGAG 1 cut(s) 136
HaeIII GGCC 4 cut(s) 267, 386, 470, 1579
HapII CCGG 2 cut(s) 1055, 1576
HhaI GCGC 2 cut(s) 844, 880
Hin1II CATG 6 cut(s) 215, 304, 607, 1351, 1456, 1726
Hin6I GCGC 2 cut(s) 842, 878
HinP1I GCGC 2 cut(s) 842, 878
HincII GTYRAC 2 cut(s) 307, 1050
HindII GTYRAC 2 cut(s) 307, 1050
HindIII AAGCTT 2 cut(s) 581, 704
HinfI GANTC 6 cut(s) 331, 482, 514, 562, 913, 1691
HpaI GTTAAC 1 cut(s) 1050
HpaII CCGG 2 cut(s) 1055, 1576
HphI GGTGA 3 cut(s) 920, 970, 1259
Hpy166II GTNNAC 9 cut(s) 101, 286, 307, 750, 1050, 1171, 1450, 1551, 1735
Hpy188I TCNGA 6 cut(s) 819, 1147, 1345, 1524, 1687, 1756
Hpy188III TCNNGA 9 cut(s) 153, 293, 369, 500, 677, 726, 973, 1055, 1237
Hpy8I GTNNAC 9 cut(s) 101, 286, 307, 750, 1050, 1171, 1450, 1551, 1735
HpyAV CCTTC 7 cut(s) 114, 592, 779, 899, 1030, 1094, 1604
HpyCH4III ACNGT 4 cut(s) 414, 640, 890, 1480
HpyF10VI GCNNNNNNNGC 3 cut(s) 264, 987, 1220
HpyF3I CTNAG 3 cut(s) 18, 734, 1755
Hsp92II CATG 6 cut(s) 215, 304, 607, 1351, 1456, 1726
HspAI GCGC 2 cut(s) 842, 878
Kpn2I TCCGGA 1 cut(s) 1054
KroI GCCGGC 1 cut(s) 1575
KroNI GCCGGC 1 cut(s) 1577
Ksp22I TGATCA 3 cut(s) 234, 450, 1216
KspAI GTTAAC 1 cut(s) 1050
Kzo9I GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
LmnI GCTCC 3 cut(s) 421, 819, 1648
Lsp1109I GCAGC 1 cut(s) 1177
LweI GCATC 5 cut(s) 817, 978, 1000, 1048, 1525
MaeI CTAG 3 cut(s) 594, 917, 1768
MaeIII GTNAC 2 cut(s) 538, 958
MalI GATC 7 cut(s) 236, 452, 609, 854, 1074, 1218, 1379
MboI GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
MfeI CAATTG 2 cut(s) 625, 1434
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 6 cut(s) 625, 1306, 1362, 1387, 1434, 1614
MlyI GAGTC 2 cut(s) 523, 571
Mph1103I ATGCAT 1 cut(s) 1063
MroI TCCGGA 1 cut(s) 1054
MroNI GCCGGC 1 cut(s) 1575
MseI TTAA 3 cut(s) 1049, 1673, 1761
MslI CAYNNNNRTG 2 cut(s) 1544, 1556
MspI CCGG 2 cut(s) 1055, 1576
MunI CAATTG 2 cut(s) 625, 1434
Mva1269I GAATGC 1 cut(s) 795
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 3 cut(s) 264, 987, 1220
NaeI GCCGGC 1 cut(s) 1577
NdeII GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
NgoMIV GCCGGC 1 cut(s) 1575
NlaIII CATG 6 cut(s) 215, 304, 607, 1351, 1456, 1726
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 958
NsiI ATGCAT 1 cut(s) 1063
PaeR7I CTCGAG 1 cut(s) 367
PcsI WCGNNNNNNNCGW 2 cut(s) 384, 1397
PctI GAATGC 1 cut(s) 795
PdiI GCCGGC 1 cut(s) 1577
PfeI GAWTC 4 cut(s) 331, 482, 913, 1691
PkrI GCNGC 2 cut(s) 992, 1192
PleI GAGTC 2 cut(s) 522, 570
PpsI GAGTC 2 cut(s) 522, 570
PpuMI RGGWCCY 1 cut(s) 1713
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 1713
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 1713
PspPPI RGGWCCY 1 cut(s) 1713
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 2 cut(s) 1544, 1556
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 1049, 1673, 1761
SatI GCNGC 2 cut(s) 991, 1191
Sau3AI GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
Sau96I GGNCC 1 cut(s) 1713
SchI GAGTC 2 cut(s) 523, 571
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 5 cut(s) 817, 978, 1000, 1048, 1525
SfcI CTRYAG 2 cut(s) 568, 1476
Sfr274I CTCGAG 1 cut(s) 367
SinI GGWCC 1 cut(s) 1713
SlaI CTCGAG 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 1544, 1556
SmlI CTYRAG 2 cut(s) 293, 367
SmoI CTYRAG 2 cut(s) 293, 367
Sse9I AATT 6 cut(s) 625, 1306, 1362, 1387, 1434, 1614
SsiI CCGC 1 cut(s) 991
SspI AATATT 3 cut(s) 405, 657, 1495
SspMI CTAG 3 cut(s) 594, 917, 1768
SstI GAGCTC 1 cut(s) 300
TaaI ACNGT 4 cut(s) 414, 640, 890, 1480
TaqI TCGA 6 cut(s) 368, 378, 807, 825, 1236, 1533
TasI AATT 6 cut(s) 625, 1306, 1362, 1387, 1434, 1614
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 4 cut(s) 331, 482, 913, 1691
Tru1I TTAA 3 cut(s) 1049, 1673, 1761
Tru9I TTAA 3 cut(s) 1049, 1673, 1761
TscAI CASTG 5 cut(s) 22, 91, 460, 1020, 1300
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 1 cut(s) 1190
Tsp45I GTSAC 1 cut(s) 958
TspDTI ATGAA 6 cut(s) 291, 426, 524, 708, 716, 1049
TspRI CASTG 5 cut(s) 22, 91, 460, 1020, 1300
VpaK11BI GGWCC 1 cut(s) 1713
XhoI CTCGAG 1 cut(s) 367
XmiI GTMKAC 4 cut(s) 100, 285, 749, 1449
XspI CTAG 3 cut(s) 594, 917, 1768
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.