MD04G1230300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009511
Physical Location & Seq
Reverse (-)
31041741 .. 31043642
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1230300.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGACAACAGTTGCCGCCAAGCTGTTGCCTCAGAGCCTCCACGTCACCACCCACCGGAACCCCTCCTCCCACCAGACCAGTGCCGTGAGCTGCAGGGTTTGCATTTCGTCAAACGGCGGAAACCACCGCAGAAACCCGCCAAAGGCGGCGGCTTTCGCCAAAGCAAGACCGACCCAGTTGCAGAACAACGATGTCAAGGAAACCCATTTGGTGAAACCCTCGTCCCGCCAAACCAATGCCTTGAGATGCGGGGTTTGCATTTCGTCCAACCCCGGAAACCGCCGCAGAAATCGGCCAAAGGCGGCGGCTTCCGCCGAAGCAAGACCGACCCAGTTGCAGAACAATGATGTCAAAGAAACCCATTTGGTGAAAGTCCTCAACAGGTCCTGCAAAGCAGGGCAGTACAATGAAGCACTCTACTTTCTTGAGCTGATGGTGAACAAGGGGTACAAGCCCGACGTGATCCTCTGCACAAAGCTCATCAAAGGGTTCTTCAATTCGAGAAACGTCGAAAAGGCCGTTCGGGTTATGCAGATTTTGGAGAAGTATGGTGAGCCTGATTTGTTTTCATACAATGCGTTGATCAGCGGGTTCTGTAAGGCGAATCGGATTGATGCAGCAAACAAAGTGCTTGATAGGATGAGGAGCCAGGGGTTCTCACCCGATGTTGTTACTTACAATATAATGATTGGGAGTCTTTGTGGGAGGGGGAAGCTAGGATTAGCTTTGAAGGTTTTGGATCAGTTGGTGAAGGATAATTGCAGGCCAACTGTGATTACTTACACGATTTTGATCGAAGCAACGATTCCTGAAGGCGGAATTGATGAAGCAATGAAGCTTTTGGATGAGATGTTGTCAAGAGGGCTTAAACCTGACATGCATACTTACAATGCAGTTGTTAGGGGAATGTGCAGAGAAGGGATGCTGGATAGGGCTTTCGAGTTTGTTAGGAGCTTAGATTCGAAGGGATGTTCGCCTAATGGAGTCTCGTACAACATTTTGTTACGAGCGCTATTGAATCGAGGTAAATGGGAAGAAGGGGAGAAGCTGGTGAGCAATATGTGTTCGAGAGGCTGTGAGCCGAACGTGGTGACTTACAGCATTTTGATTAGCACGCTGTGTCGCGATGGCAAAGTCGAGGATGCAGTGAATGTGTTAAAGATCATGAAGGACAAGGGATTGACTCCGGATGCGTATAGTTATGATCCGTTGGTTTCTGCTTTCTGCAAAGAGGGGAGATTGGATTTGGCAATAGAGTTTTTGGATTACATGATATCGGATGGCTGCTTGCCGGATATTGTGAACTACAATACGATATTGGCTGCGTTGTGTAAGAGTGGAAAAGCTGATCAGGCTTTGCAAATCTTCGAGAACCTCGGTGAAGTGGGATGTCCTCCGAATGTGAGCTCATACAACACCATGTTCAGTGCACTGTGGAACTGTGGAGACAGAGTTCGAGCTTTGCAAATGGTATCAGAAATGGTAAGCAAAGGAATTGATCCCGATGGGATTACTTACAATTCGCTTATATCGTGTTTGTGCAGAGATGGGATGGTGAATGAGGCTATAGGGTTGTTGGTAGACATGGAAACTGGTGGCTTTCAGCCAACAGTTATCAGTTACAACATTGTTCTTCTCGGGTTGTGCAAAACGCGTAGGATTGTCGATGCCATTGAAGTGCTCGCGGCAATGGTTGAAAAGGGGTGCCGGCCAAATGAAACCACCTACACTCTGTTGATTGAAGGAATTGGTTTCGCAGGATGGCGAGCTGAAGCGATGCAGCTTGCCCACTCAGTTTTTAGTATAAGGGCGATTTCCGAAGATTCGTTCAGACGTTTGAATAGAACCTTTCCCATGCTTGATGTGTACAAAGAGCTTACTCTGTCTGAGATCAAGAACTAG

Protein Analysis

634

Amino Acids

70.0

Weight (kDa)

8.74

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 143 - 198 9.1e-08 Pentatricopeptide repeat domain
PPR_3 PF13812 185 - 233 3.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 185 - 215 4.1e-11 PPR repeat
PPR_2 PF13041 186 - 234 5.9e-18 PPR repeat family
PPR PF01535 189 - 219 2e-07 PPR repeat
PPR_1 PF12854 218 - 248 6.5e-09 PPR repeat
PPR_2 PF13041 221 - 267 8.1e-15 PPR repeat family
TPR_24 PF23276 237 - 344 4.1e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 242 - 343 7.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 253 - 284 5.1e-06 PPR repeat
PPR_2 PF13041 265 - 305 7.8e-10 PPR repeat family
PPR_3 PF13812 279 - 338 5.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 287 - 317 3e-10 PPR repeat
PPR_2 PF13041 292 - 339 6.8e-12 PPR repeat family
PPR_2 PF13041 329 - 375 4.6e-16 PPR repeat family
PPR_long PF17177 347 - 464 3.4e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 349 - 409 6.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 357 - 390 7.9e-14 PPR repeat
PPR PF01535 364 - 394 1.3e-06 PPR repeat
PPR_2 PF13041 368 - 410 3.1e-10 PPR repeat family
PPR_3 PF13812 386 - 443 1.6e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 392 - 424 8.6e-10 PPR repeat
TPR_24 PF23276 398 - 490 2e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 422 - 477 4.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 427 - 457 4.3e-08 PPR repeat
PPR_2 PF13041 431 - 478 8.6e-14 PPR repeat family
PPR_2 PF13041 466 - 515 3.3e-13 PPR repeat family
PPR_3 PF13812 467 - 512 2.4e-06 Pentatricopeptide repeat domain
PPR_long PF17177 486 - 587 1.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 491 - 549 1.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 497 - 530 4.5e-12 PPR repeat
PPR PF01535 504 - 533 2.2e-07 PPR repeat
PPR_2 PF13041 504 - 550 1.3e-13 PPR repeat family
PPR_3 PF13812 525 - 584 1.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 536 - 582 3.6e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1704
AccI GTMKAC 1 cut(s) 1581
AccII CGCG 3 cut(s) 1125, 1654, 1685
AccIII TCCGGA 1 cut(s) 1186
AclWI GGATC 4 cut(s) 457, 747, 1199, 1493
AcoI YGGCCR 2 cut(s) 293, 1709
AcuI CTGAAG 2 cut(s) 831, 1791
AdeI CACNNNGTG 1 cut(s) 1119
AfaI GTAC 4 cut(s) 404, 449, 992, 1868
AfeI AGCGCT 1 cut(s) 1011
AfiI CCNNNNNNNGG 3 cut(s) 54, 142, 815
AflIII ACRYGT 1 cut(s) 1652
AgsI TTSAA 7 cut(s) 496, 730, 1018, 1676, 1697, 1742, 1840
AjiI CACGTC 2 cut(s) 43, 460
AjnI CCWGG 1 cut(s) 648
AloI GAACNNNNNNTCC 4 cut(s) 50, 82, 1437, 1469
Alw21I GWGCWC 3 cut(s) 1409, 1432, 1683
Alw26I GTCTC 2 cut(s) 991, 1440
Alw44I GTGCAC 1 cut(s) 1428
AlwI GGATC 4 cut(s) 457, 747, 1199, 1493
AlwNI CAGNNNCTG 1 cut(s) 387
Ama87I CYCGRG 1 cut(s) 1637
Aor13HI TCCGGA 1 cut(s) 1186
Aor51HI AGCGCT 1 cut(s) 1011
AoxI GGCC 4 cut(s) 293, 516, 764, 1709
ApaLI GTGCAC 1 cut(s) 1428
ApeKI GCWGC 5 cut(s) 90, 617, 1284, 1322, 1780
AspLEI GCGC 1 cut(s) 1012
AspS9I GGNCC 1 cut(s) 384
AsuC2I CCSGG 1 cut(s) 273
AsuII TTCGAA 1 cut(s) 962
AvaI CYCGRG 1 cut(s) 1637
AvaII GGWCC 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 1432
BanI GGYRCC 1 cut(s) 1704
BanII GRGCYC 1 cut(s) 1409
Bbv12I GWGCWC 3 cut(s) 1409, 1432, 1683
BbvI GCAGC 5 cut(s) 77, 629, 1271, 1309, 1792
BccI CCATC 7 cut(s) 427, 1121, 1274, 1499, 1541, 1546, 1755
BceAI ACGGC 3 cut(s) 68, 130, 503
BcgI CGANNNNNNTGC 4 cut(s) 160, 194, 316, 350
BciT130I CCWGG 1 cut(s) 650
BclI TGATCA 2 cut(s) 582, 1348
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 991, 1440
BfaI CTAG 2 cut(s) 716, 1900
BfmI CTRYAG 2 cut(s) 91, 1566
BfoI RGCGCY 1 cut(s) 1013
Bme1390I CCNGG 2 cut(s) 273, 650
Bme18I GGWCC 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 1637
BmgBI CACGTC 2 cut(s) 43, 460
BmgT120I GGNCC 1 cut(s) 384
BmiI GGNNCC 3 cut(s) 59, 647, 1706
BmrFI CCNGG 2 cut(s) 273, 650
BmrI ACTGGG 2 cut(s) 169, 325
BmsI GCATC 7 cut(s) 236, 604, 912, 1132, 1180, 1657, 1767
BmuI ACTGGG 2 cut(s) 169, 325
Bpu14I TTCGAA 1 cut(s) 962
BpuEI CTTGAG 2 cut(s) 262, 446
BpuMI CCSGG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 791
BsaJI CCNNGG 3 cut(s) 271, 649, 1375
BsaWI WCCGGW 2 cut(s) 54, 1186
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 685, 715, 1437, 1467
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 142, 815
Bse118I RCCGGY 1 cut(s) 1707
Bse1I ACTGG 4 cut(s) 78, 175, 331, 1597
Bse3DI GCAATG 2 cut(s) 837, 1695
Bse8I GATNNNNATC 1 cut(s) 791
BseAI TCCGGA 1 cut(s) 1186
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 3 cut(s) 271, 649, 1375
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 3 cut(s) 54, 142, 815
BseMI GCAATG 2 cut(s) 837, 1695
BseMII CTCAG 3 cut(s) 44, 1806, 1878
BseNI ACTGG 4 cut(s) 78, 175, 331, 1597
BseRI GAGGAG 2 cut(s) 55, 658
BseSI GKGCMC 1 cut(s) 1432
BseXI GCAGC 5 cut(s) 77, 629, 1271, 1309, 1792
BsgI GTGCAG 3 cut(s) 454, 931, 1561
Bsh1236I CGCG 3 cut(s) 1125, 1654, 1685
BshFI GGCC 4 cut(s) 295, 518, 766, 1711
BshNI GGYRCC 1 cut(s) 1704
BsiHKAI GWGCWC 3 cut(s) 1409, 1432, 1683
BsiHKCI CYCGRG 1 cut(s) 1637
BsiSI CCGG 5 cut(s) 55, 273, 1187, 1292, 1708
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 3 cut(s) 54, 142, 815
BsmAI GTCTC 2 cut(s) 991, 1440
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 4 cut(s) 295, 518, 766, 1711
BsoBI CYCGRG 1 cut(s) 1637
Bsp119I TTCGAA 1 cut(s) 962
Bsp1286I GDGCHC 3 cut(s) 1409, 1432, 1683
Bsp13I TCCGGA 1 cut(s) 1186
Bsp1407I TGTACA 1 cut(s) 1866
Bsp143I GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
Bsp68I TCGCGA 1 cut(s) 1125
BspANI GGCC 4 cut(s) 295, 518, 766, 1711
BspCNI CTCAG 3 cut(s) 43, 1805, 1879
BspEI TCCGGA 1 cut(s) 1186
BspFNI CGCG 3 cut(s) 1125, 1654, 1685
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 3 cut(s) 59, 647, 1706
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 4 cut(s) 457, 747, 1199, 1493
BspT104I TTCGAA 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 1704
BsrDI GCAATG 2 cut(s) 837, 1695
BsrFI RCCGGY 1 cut(s) 1707
BsrGI TGTACA 1 cut(s) 1866
BsrI ACTGG 4 cut(s) 78, 175, 331, 1597
BssAI RCCGGY 1 cut(s) 1707
BssECI CCNNGG 3 cut(s) 271, 649, 1375
BssMI GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 5 cut(s) 10, 772, 1434, 1442, 1612
BstAPI GCANNNNNTGC 1 cut(s) 99
BstAUI TGTACA 1 cut(s) 1866
BstBI TTCGAA 1 cut(s) 962
BstC8I GCNNGC 7 cut(s) 764, 1115, 1289, 1683, 1709, 1767, 1785
BstDEI CTNAG 4 cut(s) 30, 955, 1792, 1887
BstFNI CGCG 3 cut(s) 1125, 1654, 1685
BstH2I RGCGCY 1 cut(s) 1013
BstHHI GCGC 1 cut(s) 1012
BstKTI GATC 9 cut(s) 465, 585, 742, 795, 1164, 1207, 1351, 1501, 1893
BstMAI GTCTC 2 cut(s) 991, 1440
BstMBI GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
BstMWI GCNNNNNNNGC 5 cut(s) 99, 155, 255, 311, 1352
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 880
BstSCI CCNGG 2 cut(s) 271, 648
BstSFI CTRYAG 2 cut(s) 91, 1566
BstSLI GKGCMC 1 cut(s) 1432
BstUI CGCG 3 cut(s) 1125, 1654, 1685
BstV1I GCAGC 5 cut(s) 77, 629, 1271, 1309, 1792
BsuRI GGCC 4 cut(s) 295, 518, 766, 1711
BtgZI GCGATG 2 cut(s) 1140, 1790
BtrI CACGTC 2 cut(s) 43, 460
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 4 cut(s) 85, 1152, 1430, 1432
BtuMI TCGCGA 1 cut(s) 1125
Cac8I GCNNGC 7 cut(s) 764, 1115, 1289, 1683, 1709, 1767, 1785
CaiI CAGNNNCTG 1 cut(s) 387
CciI TCATGA 1 cut(s) 1164
CfoI GCGC 1 cut(s) 1012
Cfr10I RCCGGY 1 cut(s) 1707
Cfr13I GGNCC 1 cut(s) 384
Csp6I GTAC 4 cut(s) 403, 448, 991, 1867
CviAII CATG 6 cut(s) 877, 1165, 1270, 1420, 1585, 1855
CviQI GTAC 4 cut(s) 403, 448, 991, 1867
DdeI CTNAG 4 cut(s) 30, 955, 1792, 1887
DpnI GATC 9 cut(s) 464, 584, 741, 794, 1163, 1206, 1350, 1500, 1892
DpnII GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
DraIII CACNNNGTG 1 cut(s) 1119
EaeI YGGCCR 2 cut(s) 293, 1709
EciI GGCGGA 3 cut(s) 132, 301, 831
Ecl136II GAGCTC 1 cut(s) 1407
Eco24I GRGCYC 1 cut(s) 1409
Eco32I GATATC 1 cut(s) 1275
Eco47I GGWCC 1 cut(s) 384
Eco47III AGCGCT 1 cut(s) 1011
Eco53kI GAGCTC 1 cut(s) 1407
Eco57I CTGAAG 2 cut(s) 831, 1791
Eco88I CYCGRG 1 cut(s) 1637
EcoICRI GAGCTC 1 cut(s) 1407
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 1 cut(s) 1275
EcoT22I ATGCAT 1 cut(s) 882
EcoT38I GRGCYC 1 cut(s) 1409
FaeI CATG 6 cut(s) 880, 1168, 1273, 1423, 1588, 1858
FaqI GGGAC 1 cut(s) 208
FatI CATG 6 cut(s) 876, 1164, 1269, 1419, 1584, 1854
FauI CCCGC 4 cut(s) 144, 233, 242, 581
FbaI TGATCA 2 cut(s) 582, 1348
FblI GTMKAC 1 cut(s) 1581
FriOI GRGCYC 1 cut(s) 1409
FspBI CTAG 2 cut(s) 716, 1900
GlaI GCGC 1 cut(s) 1011
HaeII RGCGCY 1 cut(s) 1013
HaeIII GGCC 4 cut(s) 295, 518, 766, 1711
HapII CCGG 5 cut(s) 55, 273, 1187, 1292, 1708
HhaI GCGC 1 cut(s) 1012
Hin1II CATG 6 cut(s) 880, 1168, 1273, 1423, 1588, 1858
Hin6I GCGC 1 cut(s) 1010
HinP1I GCGC 1 cut(s) 1010
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 8 cut(s) 604, 694, 805, 959, 984, 1018, 1183, 1823
HpaII CCGG 5 cut(s) 55, 273, 1187, 1292, 1708
Hpy166II GTNNAC 5 cut(s) 439, 1303, 1430, 1582, 1867
Hpy188I TCNGA 8 cut(s) 33, 609, 1279, 1398, 1477, 1819, 1832, 1888
Hpy8I GTNNAC 5 cut(s) 439, 1303, 1430, 1582, 1867
Hpy99I CGWCG 2 cut(s) 461, 512
HpyAV CCTTC 8 cut(s) 724, 745, 806, 911, 958, 1031, 1162, 1736
HpyCH4III ACNGT 5 cut(s) 10, 772, 1434, 1442, 1612
HpyCH4IV ACGT 5 cut(s) 42, 459, 507, 1086, 1834
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 155, 255, 311, 1352
HpyF3I CTNAG 4 cut(s) 30, 955, 1792, 1887
HpySE526I ACGT 5 cut(s) 42, 459, 507, 1086, 1834
Hsp92II CATG 6 cut(s) 880, 1168, 1273, 1423, 1588, 1858
HspAI GCGC 1 cut(s) 1010
Kpn2I TCCGGA 1 cut(s) 1186
KroI GCCGGC 1 cut(s) 1707
KroNI GCCGGC 1 cut(s) 1709
Ksp22I TGATCA 2 cut(s) 582, 1348
Kzo9I GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
LmnI GCTCC 2 cut(s) 645, 951
Lsp1109I GCAGC 5 cut(s) 77, 629, 1271, 1309, 1792
LweI GCATC 7 cut(s) 236, 604, 912, 1132, 1180, 1657, 1767
MaeI CTAG 2 cut(s) 716, 1900
MaeII ACGT 5 cut(s) 42, 459, 507, 1086, 1834
MaeIII GTNAC 5 cut(s) 43, 670, 1002, 1090, 1619
MalI GATC 9 cut(s) 464, 584, 741, 794, 1163, 1206, 1350, 1500, 1892
MboI GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
MboII GAAGA 5 cut(s) 484, 1046, 1357, 1625, 1832
MhlI GDGCHC 3 cut(s) 1409, 1432, 1683
MluCI AATT 6 cut(s) 496, 757, 819, 1494, 1519, 1746
MluI ACGCGT 1 cut(s) 1652
MlyI GAGTC 3 cut(s) 703, 993, 1177
MmeI TCCRAC 1 cut(s) 291
Mph1103I ATGCAT 1 cut(s) 882
MroI TCCGGA 1 cut(s) 1186
MroNI GCCGGC 1 cut(s) 1707
MseI TTAA 2 cut(s) 867, 1157
MslI CAYNNNNRTG 1 cut(s) 1676
MspA1I CMGCKG 1 cut(s) 588
MspI CCGG 5 cut(s) 55, 273, 1187, 1292, 1708
MspR9I CCNGG 2 cut(s) 273, 650
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 3 cut(s) 1125, 1654, 1685
MwoI GCNNNNNNNGC 5 cut(s) 99, 155, 255, 311, 1352
NaeI GCCGGC 1 cut(s) 1709
NciI CCSGG 1 cut(s) 273
NdeII GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
NgoMIV GCCGGC 1 cut(s) 1707
NlaIII CATG 6 cut(s) 880, 1168, 1273, 1423, 1588, 1858
NlaIV GGNNCC 3 cut(s) 59, 647, 1706
NmuCI GTSAC 2 cut(s) 43, 1090
NruI TCGCGA 1 cut(s) 1125
NsiI ATGCAT 1 cut(s) 882
NspI RCATGY 1 cut(s) 880
NspV TTCGAA 1 cut(s) 962
PagI TCATGA 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 2 cut(s) 516, 1529
PdiI GCCGGC 1 cut(s) 1709
PfeI GAWTC 5 cut(s) 604, 805, 959, 1018, 1823
PleI GAGTC 3 cut(s) 702, 992, 1177
PpsI GAGTC 3 cut(s) 702, 992, 1177
PpuMI RGGWCCY 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 1409
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 3 cut(s) 59, 647, 1706
PspPI GGNCC 1 cut(s) 384
PspPPI RGGWCCY 1 cut(s) 384
PsrI GAACNNNNNNTAC 2 cut(s) 431, 463
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 387
RruI TCGCGA 1 cut(s) 1125
RsaI GTAC 4 cut(s) 404, 449, 992, 1868
RsaNI GTAC 4 cut(s) 403, 448, 991, 1867
RseI CAYNNNNRTG 1 cut(s) 1676
SacI GAGCTC 1 cut(s) 1409
SaqAI TTAA 2 cut(s) 867, 1157
Sau3AI GATC 9 cut(s) 462, 582, 739, 792, 1161, 1204, 1348, 1498, 1890
Sau96I GGNCC 1 cut(s) 384
SchI GAGTC 3 cut(s) 703, 993, 1177
ScrFI CCNGG 2 cut(s) 273, 650
SduI GDGCHC 3 cut(s) 1409, 1432, 1683
SfaNI GCATC 7 cut(s) 236, 604, 912, 1132, 1180, 1657, 1767
SfcI CTRYAG 2 cut(s) 91, 1566
SfuI TTCGAA 1 cut(s) 962
SinI GGWCC 1 cut(s) 384
SmiMI CAYNNNNRTG 1 cut(s) 1676
SmlI CTYRAG 2 cut(s) 241, 425
SmoI CTYRAG 2 cut(s) 241, 425
Sse9I AATT 6 cut(s) 496, 757, 819, 1494, 1519, 1746
SspMI CTAG 2 cut(s) 716, 1900
SstI GAGCTC 1 cut(s) 1409
StyD4I CCNGG 2 cut(s) 271, 648
TaaI ACNGT 5 cut(s) 10, 772, 1434, 1442, 1612
TaiI ACGT 5 cut(s) 45, 462, 510, 1089, 1837
TaqII GACCGA 2 cut(s) 184, 340
TasI AATT 6 cut(s) 496, 757, 819, 1494, 1519, 1746
TatI WGTACW 2 cut(s) 402, 1866
TauI GCSGC 7 cut(s) 17, 149, 152, 285, 305, 308, 1688
TfiI GAWTC 5 cut(s) 604, 805, 959, 1018, 1823
Tru1I TTAA 2 cut(s) 867, 1157
Tru9I TTAA 2 cut(s) 867, 1157
TscAI CASTG 4 cut(s) 85, 1152, 1432, 1437
TseFI GTSAC 2 cut(s) 43, 1090
TseI GCWGC 5 cut(s) 90, 617, 1284, 1322, 1780
Tsp45I GTSAC 2 cut(s) 43, 1090
TspDTI ATGAA 6 cut(s) 423, 558, 840, 848, 1181, 1731
TspGWI ACGGA 1 cut(s) 1197
TspRI CASTG 4 cut(s) 85, 1152, 1432, 1437
VneI GTGCAC 1 cut(s) 1428
VpaK11BI GGWCC 1 cut(s) 384
XceI RCATGY 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 1581
XspI CTAG 2 cut(s) 716, 1900
Zsp2I ATGCAT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.