RLG00000025811

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009511
Physical Location & Seq
Forward (+)
48436116 .. 48437882
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025811

Sequence Viewer

Length: 1767 bp
ATGGCAATAGTCTCCACTGAGTTATTGCCTCATAGCTTCCACACTACCTCCCAACTCAAACCCACTTCCCATTCCCACCACCCCACTACTTTGAGTTGCAGACCTTCTTCTTCCTCCTCTTCATTCTCAAATGGCAGAAACAACACCTCCAGAAACCTAACAAAGGTGTCTGTCTCTGCTGAACTAAAACCAACACACTTGCAAAACCATGATTACAAAGAGACCCACTTGATCAAAGTCCTCAACAGGTCTTGCAAAGCAGGGCAGTACAATGAAGCCTTCTACTTTCTTGAGCTCATGGTCAACAAGGGCTACAAGCCTGATGTGATTCTCTGCACCAAGCTCATCAAAGGGTTCTTCAACTCGAGAAACATCGAAAAGGCCATTCGGGTTATGCAAATATTGGAACAGTGTGGGGAGCCTGATTTGTTTTCATACAATGCTTTGATCAGTGGGTTTTGTAAGGCCAATAGGATTGAATCAGCAAACAAAGTTCTTGATAGAATGAAGTGTCAAGGGTTCAAGCCTGATGTTGTTACATATAATATAATGATTGGGAGTCTTTGTAGTAGAGGGAAGCTTGGGTTAGCTAGGAAGGTTATGGATCGTTTAGTAAAAGACAATTGCAAGCCCACTGTGATTACATATACAATATTGATAGAAGCTACTATTCTTGATGGTGGGATTGATGAAGCTATGAAGCTTTTGGATGAGATGTTGTCAAGAGGGCTTAGACCTGATATGTATACTTACAATGCCATTGTTAGGGGAATGTGCAGGGAAGGAATGCTGGATAGAGCTTTCGAGTTTGTTAGGAGCTTCGATGCTAAAGGCTGTGCGCCGAATGTGATCTCATACAACATATTGTTGCGTGCGCTTTTGAACCGTGGGAAATGGGAGGAAGGAGAGAATCTAGTGAGCAATATGTGTGCAAGAGGTTGTGAGCCTAATGTGGTGACTTATAGCATCTTGATTAGCACGCTGTGCCGCGATGGGAAAGTTGAGGATGCAGTGAACGTGTTGAAGATTATGAAGGAAAAGGGGTTAACTCCAGATGCTTATAGTTATGATCCATTGATTTCAAGTTTCTGCAAAGAAGGGAGGTTAGATTTAGCAATAGAGTTTTTGGATTGTATGATATCTGATGGTTGCTTGCCAGATATTGTGAACTACAATACAATCTTGGCTGCTTTGTGCAAGAATGGGAGTGCTGATCAGGCTTTGGAAATCTTCGAGAACTTGGGTGAAGTCGGTTGTCCTCCAAATGTGAGTTCCTACAACACAATGTTCAGTGCATTGTGGAATTGTGGAGACAGAGTAAGAGCTTTGGGAATGGTATCAGACATGGTAAGCAAAGGAATTGAGCCTGATGAGATCACATACAATTCGCTTATATCGTGTTTGTGCAGAGATGGGATGGTAAATGAGGCTATTGGGTTGTTGGTAGACATGGAAATTGGTGGTTTTCAGCCTACAGTTATCACTTACAATATTGTTCTTCTTGGGTTGAGCAAGGCTCGGAGGATTATCGATGCCATTGAAGTGTTCACAGCAATGGTTGAAAAGGGTTGCCGGCCTAACGAAACTACTTACATTTTGCTGATTGAAGGAATTGGATTTGCAGGATGGCGAGCTGAAGCTATGGAGCTTGCAAAATCTGTTTATAGTTTAAGGGCTATTTCCGAAGATTCTTTCAAACGCTTGAACAGGACCTTTCCCATGCTTGATGTTTACAAAGAGCTTACTCTGTCTGAGATTAAGAACTAG

Protein Analysis

589

Amino Acids

65.54

Weight (kDa)

6.72

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 98 - 153 2.8e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 140 - 187 5.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 140 - 170 4.1e-11 PPR repeat
PPR_2 PF13041 141 - 189 1.2e-18 PPR repeat family
PPR PF01535 144 - 174 1.7e-07 PPR repeat
PPR_3 PF13812 164 - 222 1.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 173 - 203 3.9e-09 PPR repeat
PPR_2 PF13041 176 - 222 1.7e-14 PPR repeat family
TPR_24 PF23276 192 - 300 3.9e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 197 - 298 4.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 208 - 239 2.1e-07 PPR repeat
PPR_2 PF13041 227 - 260 1.7e-09 PPR repeat family
PPR_3 PF13812 234 - 293 4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 242 - 272 7.7e-11 PPR repeat
PPR_2 PF13041 250 - 294 9.4e-11 PPR repeat family
PPR_2 PF13041 281 - 330 5.9e-17 PPR repeat family
PPR_long PF17177 304 - 420 4.7e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 305 - 364 7.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 312 - 345 7.2e-14 PPR repeat
PPR PF01535 319 - 349 4.8e-07 PPR repeat
PPR_2 PF13041 323 - 360 1.8e-08 PPR repeat family
PPR_1 PF12854 347 - 379 2.2e-10 PPR repeat
TPR_24 PF23276 353 - 445 3.4e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 379 - 432 9.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 382 - 412 4.1e-08 PPR repeat
PPR_2 PF13041 386 - 433 2.2e-13 PPR repeat family
PPR_2 PF13041 421 - 470 1.5e-13 PPR repeat family
PPR_long PF17177 443 - 536 3.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 446 - 500 6.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 452 - 485 2.3e-13 PPR repeat
PPR_2 PF13041 456 - 505 4.1e-14 PPR repeat family
PPR PF01535 459 - 486 4.8e-07 PPR repeat
PPR_3 PF13812 480 - 539 1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 491 - 537 2e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 746, 1446
AccII CGCG 1 cut(s) 990
AciI CCGC 1 cut(s) 988
AclWI GGATC 2 cut(s) 612, 1064
AcuI CTGAAG 1 cut(s) 1656
AdeI CACNNNGTG 1 cut(s) 984
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 2 cut(s) 163, 765
AflIII ACRYGT 1 cut(s) 1017
AjuI GAANNNNNNNTTGG 2 cut(s) 1255, 1287
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 4 cut(s) 16, 178, 215, 1305
AlwI GGATC 2 cut(s) 612, 1064
Ama87I CYCGRG 1 cut(s) 364
AoxI GGCC 3 cut(s) 381, 465, 1574
ApeKI GCWGC 1 cut(s) 1187
AspLEI GCGC 2 cut(s) 841, 877
AspS9I GGNCC 1 cut(s) 1710
AsuHPI GGTGA 2 cut(s) 967, 1256
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 1 cut(s) 1710
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 1174
BccI CCATC 6 cut(s) 671, 986, 1139, 1406, 1411, 1620
BclI TGATCA 3 cut(s) 231, 447, 1213
BcoDI GTCTC 4 cut(s) 16, 178, 215, 1305
BfaI CTAG 3 cut(s) 591, 914, 1765
BfmI CTRYAG 1 cut(s) 1473
BisI GCNGC 2 cut(s) 988, 1188
BlsI GCNGC 2 cut(s) 989, 1189
Bme18I GGWCC 1 cut(s) 1710
BmeT110I CYCGRG 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 1710
BmiI GGNNCC 1 cut(s) 420
BmsI GCATC 5 cut(s) 814, 975, 997, 1045, 1522
BplI GAGNNNNNCTC 2 cut(s) 1501, 1533
BpmI CTGGAG 2 cut(s) 133, 1035
BpuEI CTTGAG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 1530
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 886
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 131, 161, 550, 580, 1302, 1332
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 765
Bse118I RCCGGY 1 cut(s) 1572
Bse3DI GCAATG 1 cut(s) 1560
BseCI ATCGAT 1 cut(s) 1530
BseDI CCNNGG 1 cut(s) 886
BseGI GGATG 4 cut(s) 715, 1012, 1422, 1631
BseLI CCNNNNNNNGG 2 cut(s) 163, 765
BseMI GCAATG 1 cut(s) 1560
BseMII CTCAG 2 cut(s) 9, 1743
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 1174
BsgI GTGCAG 3 cut(s) 319, 796, 1426
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 3 cut(s) 383, 467, 1576
BshVI ATCGAT 1 cut(s) 1530
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 364
BsiSI CCGG 1 cut(s) 1573
BslI CCNNNNNNNGG 2 cut(s) 163, 765
BsmAI GTCTC 4 cut(s) 16, 178, 215, 1305
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 3 cut(s) 383, 467, 1576
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 364
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
BspACI CCGC 1 cut(s) 988
BspANI GGCC 3 cut(s) 383, 467, 1576
BspCNI CTCAG 2 cut(s) 10, 1744
BspDI ATCGAT 1 cut(s) 1530
BspFNI CGCG 1 cut(s) 990
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 2 cut(s) 612, 1064
BspTNI GGTCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 1560
BsrFI RCCGGY 1 cut(s) 1572
BssAI RCCGGY 1 cut(s) 1572
BssECI CCNNGG 1 cut(s) 886
BssMI GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
BssNAI GTATAC 1 cut(s) 747
Bst1107I GTATAC 1 cut(s) 747
Bst4CI ACNGT 4 cut(s) 411, 637, 887, 1477
Bst6I CTCTTC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 984
BstC8I GCNNGC 7 cut(s) 629, 873, 980, 1154, 1574, 1632, 1650
BstDEI CTNAG 3 cut(s) 18, 731, 1752
BstDSI CCRYGG 1 cut(s) 886
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 4 cut(s) 715, 1012, 1422, 1631
BstFNI CGCG 1 cut(s) 990
BstHHI GCGC 2 cut(s) 841, 877
BstKTI GATC 7 cut(s) 234, 450, 607, 852, 1072, 1216, 1377
BstMAI GTCTC 4 cut(s) 16, 178, 215, 1305
BstMBI GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
BstMWI GCNNNNNNNGC 2 cut(s) 984, 1217
BstSFI CTRYAG 1 cut(s) 1473
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 1 cut(s) 1174
BstZ17I GTATAC 1 cut(s) 747
Bsu15I ATCGAT 1 cut(s) 1530
BsuRI GGCC 3 cut(s) 383, 467, 1576
BsuTUI ATCGAT 1 cut(s) 1530
BtgI CCRYGG 1 cut(s) 886
BtgZI GCGATG 1 cut(s) 1005
BtsCI GGATG 4 cut(s) 715, 1012, 1422, 1631
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 6 cut(s) 15, 416, 457, 633, 1017, 1297
Cac8I GCNNGC 7 cut(s) 629, 873, 980, 1154, 1574, 1632, 1650
CfoI GCGC 2 cut(s) 841, 877
Cfr10I RCCGGY 1 cut(s) 1572
Cfr13I GGNCC 1 cut(s) 1710
ClaI ATCGAT 1 cut(s) 1530
Csp6I GTAC 1 cut(s) 268
CviAII CATG 5 cut(s) 209, 298, 1345, 1450, 1720
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 18, 731, 1752
DpnI GATC 7 cut(s) 233, 449, 606, 851, 1071, 1215, 1376
DpnII GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
DraIII CACNNNGTG 1 cut(s) 984
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 1140
Eco47I GGWCC 1 cut(s) 1710
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 1656
Eco88I CYCGRG 1 cut(s) 364
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 1710
EcoRV GATATC 1 cut(s) 1140
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 5 cut(s) 212, 301, 1348, 1453, 1723
FatI CATG 5 cut(s) 208, 297, 1344, 1449, 1719
FbaI TGATCA 3 cut(s) 231, 447, 1213
FblI GTMKAC 2 cut(s) 746, 1446
Fnu4HI GCNGC 2 cut(s) 988, 1188
FokI GGATG 4 cut(s) 722, 1019, 1429, 1638
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 988, 1188
FspBI CTAG 3 cut(s) 591, 914, 1765
GlaI GCGC 2 cut(s) 840, 876
GluI GCNGC 2 cut(s) 988, 1188
GsuI CTGGAG 2 cut(s) 133, 1035
HaeIII GGCC 3 cut(s) 383, 467, 1576
HapII CCGG 1 cut(s) 1573
HhaI GCGC 2 cut(s) 841, 877
Hin1II CATG 5 cut(s) 212, 301, 1348, 1453, 1723
Hin6I GCGC 2 cut(s) 839, 875
HinP1I GCGC 2 cut(s) 839, 875
HincII GTYRAC 2 cut(s) 304, 1047
HindII GTYRAC 2 cut(s) 304, 1047
HindIII AAGCTT 2 cut(s) 578, 701
HinfI GANTC 5 cut(s) 328, 479, 559, 910, 1688
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 1 cut(s) 1573
HphI GGTGA 2 cut(s) 967, 1256
Hpy166II GTNNAC 8 cut(s) 304, 747, 1015, 1047, 1168, 1447, 1548, 1732
Hpy188I TCNGA 5 cut(s) 1144, 1342, 1521, 1684, 1753
Hpy188III TCNNGA 9 cut(s) 150, 290, 366, 497, 674, 723, 970, 1052, 1234
Hpy8I GTNNAC 8 cut(s) 304, 747, 1015, 1047, 1168, 1447, 1548, 1732
HpyAV CCTTC 8 cut(s) 114, 289, 589, 776, 896, 1027, 1091, 1601
HpyCH4III ACNGT 4 cut(s) 411, 637, 887, 1477
HpyCH4IV ACGT 1 cut(s) 1017
HpyF10VI GCNNNNNNNGC 2 cut(s) 984, 1217
HpyF3I CTNAG 3 cut(s) 18, 731, 1752
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 5 cut(s) 212, 301, 1348, 1453, 1723
HspAI GCGC 2 cut(s) 839, 875
KroI GCCGGC 1 cut(s) 1572
KroNI GCCGGC 1 cut(s) 1574
Ksp22I TGATCA 3 cut(s) 231, 447, 1213
KspAI GTTAAC 1 cut(s) 1047
Kzo9I GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
LmnI GCTCC 3 cut(s) 418, 816, 1645
Lsp1109I GCAGC 1 cut(s) 1174
LweI GCATC 5 cut(s) 814, 975, 997, 1045, 1522
MaeI CTAG 3 cut(s) 591, 914, 1765
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 2 cut(s) 535, 955
MalI GATC 7 cut(s) 233, 449, 606, 851, 1071, 1215, 1376
MboI GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
MboII GAAGA 8 cut(s) 99, 102, 111, 349, 1036, 1222, 1490, 1697
MfeI CAATTG 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 6 cut(s) 622, 1303, 1359, 1384, 1455, 1611
MlyI GAGTC 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 1572
MseI TTAA 3 cut(s) 1046, 1670, 1758
MslI CAYNNNNRTG 2 cut(s) 1541, 1553
MspI CCGG 1 cut(s) 1573
MunI CAATTG 1 cut(s) 622
Mva1269I GAATGC 1 cut(s) 792
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 2 cut(s) 984, 1217
NaeI GCCGGC 1 cut(s) 1574
NdeII GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
NgoMIV GCCGGC 1 cut(s) 1572
NlaIII CATG 5 cut(s) 212, 301, 1348, 1453, 1723
NlaIV GGNNCC 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 955
PaeR7I CTCGAG 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 1394
PctI GAATGC 1 cut(s) 792
PdiI GCCGGC 1 cut(s) 1574
PfeI GAWTC 4 cut(s) 328, 479, 910, 1688
PkrI GCNGC 2 cut(s) 989, 1189
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
PpuMI RGGWCCY 1 cut(s) 1710
Psp124BI GAGCTC 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 1710
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 1 cut(s) 1710
PspPPI RGGWCCY 1 cut(s) 1710
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 2 cut(s) 1541, 1553
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 1046, 1670, 1758
SatI GCNGC 2 cut(s) 988, 1188
Sau3AI GATC 7 cut(s) 231, 447, 604, 849, 1069, 1213, 1374
Sau96I GGNCC 1 cut(s) 1710
SchI GAGTC 1 cut(s) 568
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 5 cut(s) 814, 975, 997, 1045, 1522
SfcI CTRYAG 1 cut(s) 1473
Sfr274I CTCGAG 1 cut(s) 364
SinI GGWCC 1 cut(s) 1710
SlaI CTCGAG 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 1541, 1553
SmlI CTYRAG 2 cut(s) 290, 364
SmoI CTYRAG 2 cut(s) 290, 364
Sse9I AATT 6 cut(s) 622, 1303, 1359, 1384, 1455, 1611
SsiI CCGC 1 cut(s) 988
SspI AATATT 3 cut(s) 402, 654, 1492
SspMI CTAG 3 cut(s) 591, 914, 1765
SstI GAGCTC 1 cut(s) 297
TaaI ACNGT 4 cut(s) 411, 637, 887, 1477
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 6 cut(s) 365, 375, 804, 822, 1233, 1530
TasI AATT 6 cut(s) 622, 1303, 1359, 1384, 1455, 1611
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 990
TfiI GAWTC 4 cut(s) 328, 479, 910, 1688
Tru1I TTAA 3 cut(s) 1046, 1670, 1758
Tru9I TTAA 3 cut(s) 1046, 1670, 1758
TscAI CASTG 6 cut(s) 22, 416, 457, 640, 1017, 1297
TseFI GTSAC 1 cut(s) 955
TseI GCWGC 1 cut(s) 1187
Tsp45I GTSAC 1 cut(s) 955
TspDTI ATGAA 7 cut(s) 111, 288, 423, 521, 705, 713, 1046
TspRI CASTG 6 cut(s) 22, 416, 457, 640, 1017, 1297
VpaK11BI GGWCC 1 cut(s) 1710
XagI CCTNNNNNAGG 1 cut(s) 161
XhoI CTCGAG 1 cut(s) 364
XmiI GTMKAC 2 cut(s) 746, 1446
XspI CTAG 3 cut(s) 591, 914, 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.