MD12G1247400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009511
Physical Location & Seq
Reverse (-)
31779221 .. 31781119
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1247400.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGACAACAGTTGCCGCCAAGCTGTTGCCTCAGAGCCTCCACGACACCACCCACCCGAATCCTTCCTCCCACCAAACCAGCGCCGGGAGCTGCAGGGTTTGCATTCTATCAAACGGCGGAAACCGCCGCAGAAACCCGCCAAAGGCGGCTGCTTCCGCCGAAGCGAAACCGACCCATTTGCAGAACCATAACATCAAAGAAACCCATTTGTTGAAACCGTCTTCCCGCCAAACGAGTGCCTTGAGTTGCAGGGTTTGCATTTCGTCCAACGGAAACCGCCGCAGAAAACCACCAAAGGCGGCGGCTTCCGCCGAAGTAAGAGCGACCCAGTTGCAGAACAATGATTCCAAAGAAACCCATTTGATGAAAGTTCTCGACAGGTCCTGCAAAGCAGGACAGTACAATGAAGCACTCTACTTTCTTGAGCTAATGGTTAACGAGGGGTACAGGCCTGATGTGATTCTCTACACAAAGCTCATCAAAGGGTTCTTCAATTCGAGGAACGTCGAAAAGGCAGTTCGGGTTATGCAGATTTTGGAGAAGTATGGTGAGCCTGATTTGTTTTCGTACAATGCGTTGATCAGCGGGTTCTGTAAGGCGAATCGGATTGATTCAGCAAACAAGGTACTTGATAGGATGAGGAGTCGGGGATTTTCACCTGATGTTGTTACTTACAATATCATGATTGGGAGTCTTTGTGGCAGGGGCAAGCTAGGATTAGCTTTGAAGGTTTTGGATCAGTTGGTGAAGGATAATTGCAGGCCGACTGTGATTACTTACATGATTTTGATCGAAGCAATGATTCTTGAAGGCGGAATTGATGAGGCGGTGAATCTTTTGGATGACATGTTGTCTGGAGGGCTTAAACCTGACATGAATACTTACAATGCAGTTGTTAAGGGAATGTGTAGAGAAGGGATGCTGGATAGGGCGTTCGGCTTAGTTAGGAGCTTAGATTCAAAGGGTTGTTCGCCTAATGTAGTCTCGTACAACATTTTGTTACGAGCGCTTTTGAATAGGGGTAAATGGGAGGAAGGGGAGAAGCTAGTGAGCAATATGTATTCGAGAGGTTGTGAACCGAATGTGGTGACTTACAGCATTTTGATTAGCACGCTTTGTCGCCATGGGAAAGTTGAGGATGCTGTGAATGTGCTGAAGATCATGAAGGACAAGGGATTGACGCCGGATGCGTATAGTTATGATCCGCTGGTTTCTGCATTCTGCAAAGAGGGGAGATTGGATTTGGCGATAGAGTTTTTGGATTACATGATATCGGATGGCTGCTTGCCGGATATTGTGAACTACAATACAATCTTGGCTGCTTTGTGTAAGAGTGGAAAAGCTGATCAGGCTTTGGAAATCTTCGAGAACCCCGGTGAAGTGGGATGTCCTCCGAATGTGAGCTCTTACAACACGATGTTCAGTGCACTGTGGAATTGCGGAGATAGAGTGAGAGCGCTTCAAATGGTGTCGGAGATGGTAAGCAAAGGAATTGACCCCGATGAGATTACTTACAATTCGCTTATATCGTGTTTGTGCAGAGACAGGATGGTGAATGAGGCTATAGGGTTGTTGGTAGACATGGAAAGTGGTGGCTTTCAGCCAACAGTTATCAGTTACAACATTGTTGTTCTCGGGCTGTGCAAAACGCGTAGGATTGTCGATGCCATTGAAGTGCTTGCGGCAATGGTTGAAAAGGGGTGCCGGCCTAATGAAACTACCTACACTTTGTTGATTGAAGGAATTGGATTCGCAGGATGGCGAGCTGAAGCGATGGAGCTTGCCAACTCTGTTTTTAGTTTAAGGGCGATTTCCGAAGATTCGTTCGGGCGTTTGAATAGAACCTTTCCCATGCTTGATGTTTACAAAGAGCTTACTCTATCTGAGATTAAGAACTAG

Protein Analysis

633

Amino Acids

69.84

Weight (kDa)

8.24

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 142 - 195 1.9e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 184 - 232 1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 184 - 214 1.7e-11 PPR repeat
PPR_2 PF13041 185 - 233 2.3e-18 PPR repeat family
PPR PF01535 188 - 218 4.3e-08 PPR repeat
PPR_3 PF13812 208 - 266 3.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 216 - 247 4.3e-09 PPR repeat
PPR_2 PF13041 220 - 267 9.6e-14 PPR repeat family
TPR_24 PF23276 236 - 344 9.5e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 257 - 304 2.2e-11 PPR repeat family
PPR_3 PF13812 279 - 337 2.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 287 - 316 3e-09 PPR repeat
PPR_2 PF13041 294 - 338 4.4e-12 PPR repeat family
PPR_2 PF13041 325 - 374 3.9e-18 PPR repeat family
PPR_long PF17177 346 - 457 1.2e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 348 - 408 3.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 356 - 389 8.4e-14 PPR repeat
PPR PF01535 363 - 393 4.1e-07 PPR repeat
PPR_2 PF13041 367 - 409 1.5e-10 PPR repeat family
TPR_24 PF23276 370 - 489 3e-12 Fungal tetratrico peptide repeats
PPR_3 PF13812 388 - 442 4.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 391 - 423 8.6e-10 PPR repeat
PPR_3 PF13812 421 - 476 4.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 426 - 456 3.6e-08 PPR repeat
PPR_2 PF13041 430 - 477 3.1e-12 PPR repeat family
PPR_2 PF13041 465 - 514 5.6e-14 PPR repeat family
PPR_long PF17177 485 - 580 5.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 496 - 529 2.5e-11 PPR repeat
PPR_2 PF13041 500 - 549 3.3e-14 PPR repeat family
PPR PF01535 503 - 533 8.9e-07 PPR repeat
PPR_3 PF13812 524 - 583 9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 535 - 581 1.5e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1701
AccI GTMKAC 1 cut(s) 1578
AccII CGCG 1 cut(s) 1651
AclWI GGATC 2 cut(s) 744, 1196
AcuI CTGAAG 2 cut(s) 1175, 1788
AcyI GRCGYC 1 cut(s) 1181
AfaI GTAC 5 cut(s) 401, 446, 569, 627, 989
AfeI AGCGCT 2 cut(s) 1008, 1458
AfiI CCNNNNNNNGG 2 cut(s) 84, 142
AflIII ACRYGT 2 cut(s) 846, 1649
AhdI GACNNNNNGTC 1 cut(s) 850
AloI GAACNNNNNNTCC 4 cut(s) 329, 361, 917, 949
Alw21I GWGCWC 2 cut(s) 1406, 1429
Alw26I GTCTC 2 cut(s) 988, 1536
Alw44I GTGCAC 1 cut(s) 1425
AlwI GGATC 2 cut(s) 744, 1196
AlwNI CAGNNNCTG 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 1634
Aor51HI AGCGCT 2 cut(s) 1008, 1458
AoxI GGCC 3 cut(s) 449, 761, 1706
ApaLI GTGCAC 1 cut(s) 1425
ApeKI GCWGC 4 cut(s) 90, 149, 1281, 1319
AspLEI GCGC 3 cut(s) 83, 1009, 1459
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 2 cut(s) 85, 1374
AsuHPI GGTGA 7 cut(s) 560, 648, 757, 841, 1099, 1388, 1564
AvaI CYCGRG 1 cut(s) 1634
AvaII GGWCC 1 cut(s) 381
BaeGI GKGCMC 1 cut(s) 1429
BanI GGYRCC 1 cut(s) 1701
BanII GRGCYC 1 cut(s) 1406
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 1406, 1429
BbvI GCAGC 4 cut(s) 77, 136, 1268, 1306
BccI CCATC 5 cut(s) 1271, 1471, 1543, 1752, 1768
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 4 cut(s) 160, 194, 313, 347
BclI TGATCA 2 cut(s) 579, 1345
BcnI CCSGG 2 cut(s) 85, 1374
BcoDI GTCTC 2 cut(s) 988, 1536
BfaI CTAG 3 cut(s) 713, 1046, 1897
BfmI CTRYAG 2 cut(s) 91, 1563
BfoI RGCGCY 3 cut(s) 84, 1010, 1460
Bme1390I CCNGG 2 cut(s) 85, 1374
Bme18I GGWCC 1 cut(s) 381
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 1634
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 1 cut(s) 1703
BmrFI CCNGG 2 cut(s) 85, 1374
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 4 cut(s) 909, 1129, 1177, 1654
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 262, 443
BpuMI CCSGG 2 cut(s) 85, 1374
BsaBI GATNNNNATC 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 1181
BsaJI CCNNGG 2 cut(s) 1123, 1372
BsaXI ACNNNNNCTCC 4 cut(s) 682, 712, 1434, 1464
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 142
Bse118I RCCGGY 1 cut(s) 1704
Bse1I ACTGG 1 cut(s) 328
Bse3DI GCAATG 2 cut(s) 804, 1692
Bse8I GATNNNNATC 1 cut(s) 788
BseDI CCNNGG 2 cut(s) 1123, 1372
BseGI GGATG 9 cut(s) 642, 847, 924, 1144, 1192, 1282, 1391, 1554, 1763
BseJI GATNNNNATC 1 cut(s) 788
BseLI CCNNNNNNNGG 2 cut(s) 84, 142
BseMI GCAATG 2 cut(s) 804, 1692
BseMII CTCAG 2 cut(s) 44, 1875
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 655
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 4 cut(s) 77, 136, 1268, 1306
BsgI GTGCAG 1 cut(s) 1558
Bsh1236I CGCG 1 cut(s) 1651
BshFI GGCC 3 cut(s) 451, 763, 1708
BshNI GGYRCC 1 cut(s) 1701
BsiHKAI GWGCWC 2 cut(s) 1406, 1429
BsiHKCI CYCGRG 1 cut(s) 1634
BsiSI CCGG 5 cut(s) 84, 1184, 1289, 1374, 1705
BslI CCNNNNNNNGG 2 cut(s) 84, 142
BsmAI GTCTC 2 cut(s) 988, 1536
BsmI GAATGC 2 cut(s) 102, 1217
BsnI GGCC 3 cut(s) 451, 763, 1708
BsoBI CYCGRG 1 cut(s) 1634
Bsp1286I GDGCHC 2 cut(s) 1406, 1429
Bsp143I GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
Bsp19I CCATGG 1 cut(s) 1123
BspANI GGCC 3 cut(s) 451, 763, 1708
BspCNI CTCAG 2 cut(s) 43, 1876
BspFNI CGCG 1 cut(s) 1651
BspHI TCATGA 2 cut(s) 681, 1161
BspLI GGNNCC 1 cut(s) 1703
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 2 cut(s) 744, 1196
BspT107I GGYRCC 1 cut(s) 1701
BsrDI GCAATG 2 cut(s) 804, 1692
BsrFI RCCGGY 1 cut(s) 1704
BsrI ACTGG 1 cut(s) 328
BssAI RCCGGY 1 cut(s) 1704
BssECI CCNNGG 2 cut(s) 1123, 1372
BssMI GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
BssNI GRCGYC 1 cut(s) 1181
BssT1I CCWWGG 1 cut(s) 1123
Bst4CI ACNGT 6 cut(s) 10, 219, 399, 769, 1431, 1609
BstACI GRCGYC 1 cut(s) 1181
BstAPI GCANNNNNTGC 2 cut(s) 99, 255
BstC8I GCNNGC 8 cut(s) 710, 761, 1112, 1286, 1680, 1706, 1764, 1782
BstDEI CTNAG 4 cut(s) 30, 940, 952, 1884
BstDSI CCRYGG 1 cut(s) 1123
BstF5I GGATG 9 cut(s) 642, 847, 924, 1144, 1192, 1282, 1391, 1554, 1763
BstFNI CGCG 1 cut(s) 1651
BstH2I RGCGCY 3 cut(s) 84, 1010, 1460
BstHHI GCGC 3 cut(s) 83, 1009, 1459
BstKTI GATC 6 cut(s) 582, 739, 792, 1161, 1204, 1348
BstMAI GTCTC 2 cut(s) 988, 1536
BstMBI GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
BstMWI GCNNNNNNNGC 7 cut(s) 87, 99, 123, 155, 255, 308, 1349
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 2 cut(s) 83, 1372
BstSFI CTRYAG 2 cut(s) 91, 1563
BstSLI GKGCMC 1 cut(s) 1429
BstUI CGCG 1 cut(s) 1651
BstV1I GCAGC 4 cut(s) 77, 136, 1268, 1306
BstV2I GAAGAC 1 cut(s) 213
BsuRI GGCC 3 cut(s) 451, 763, 1708
BtgI CCRYGG 1 cut(s) 1123
BtgZI GCGATG 1 cut(s) 1787
BtsCI GGATG 9 cut(s) 642, 847, 924, 1144, 1192, 1282, 1391, 1554, 1763
BtsIMutI CAGTG 2 cut(s) 1427, 1429
Cac8I GCNNGC 8 cut(s) 710, 761, 1112, 1286, 1680, 1706, 1764, 1782
CaiI CAGNNNCTG 1 cut(s) 384
CciI TCATGA 2 cut(s) 681, 1161
CfoI GCGC 3 cut(s) 83, 1009, 1459
Cfr10I RCCGGY 1 cut(s) 1704
Cfr13I GGNCC 1 cut(s) 381
CseI GACGC 1 cut(s) 1189
Csp6I GTAC 5 cut(s) 400, 445, 568, 626, 988
CviAII CATG 9 cut(s) 682, 781, 847, 874, 1124, 1162, 1267, 1582, 1852
CviQI GTAC 5 cut(s) 400, 445, 568, 626, 988
DdeI CTNAG 4 cut(s) 30, 940, 952, 1884
DpnI GATC 6 cut(s) 581, 738, 791, 1160, 1203, 1347
DpnII GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
DriI GACNNNNNGTC 1 cut(s) 850
Eam1105I GACNNNNNGTC 1 cut(s) 850
EciI GGCGGA 4 cut(s) 132, 145, 298, 828
Ecl136II GAGCTC 1 cut(s) 1404
Eco130I CCWWGG 1 cut(s) 1123
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 1406
Eco32I GATATC 1 cut(s) 1272
Eco47I GGWCC 1 cut(s) 381
Eco47III AGCGCT 2 cut(s) 1008, 1458
Eco53kI GAGCTC 1 cut(s) 1404
Eco57I CTGAAG 2 cut(s) 1175, 1788
Eco88I CYCGRG 1 cut(s) 1634
EcoICRI GAGCTC 1 cut(s) 1404
EcoO109I RGGNCCY 1 cut(s) 381
EcoRV GATATC 1 cut(s) 1272
EcoT14I CCWWGG 1 cut(s) 1123
EcoT38I GRGCYC 1 cut(s) 1406
ErhI CCWWGG 1 cut(s) 1123
FaeI CATG 9 cut(s) 685, 784, 850, 877, 1127, 1165, 1270, 1585, 1855
FatI CATG 9 cut(s) 681, 780, 846, 873, 1123, 1161, 1266, 1581, 1851
FauI CCCGC 3 cut(s) 144, 233, 578
FbaI TGATCA 2 cut(s) 579, 1345
FblI GTMKAC 1 cut(s) 1578
FokI GGATG 9 cut(s) 649, 854, 931, 1151, 1199, 1289, 1398, 1561, 1770
FriOI GRGCYC 1 cut(s) 1406
FspBI CTAG 3 cut(s) 713, 1046, 1897
GlaI GCGC 3 cut(s) 82, 1008, 1458
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 3 cut(s) 84, 1010, 1460
HaeIII GGCC 3 cut(s) 451, 763, 1708
HapII CCGG 5 cut(s) 84, 1184, 1289, 1374, 1705
HgaI GACGC 1 cut(s) 1189
HhaI GCGC 3 cut(s) 83, 1009, 1459
Hin1I GRCGYC 1 cut(s) 1181
Hin1II CATG 9 cut(s) 685, 784, 850, 877, 1127, 1165, 1270, 1585, 1855
Hin6I GCGC 3 cut(s) 81, 1007, 1457
HinP1I GCGC 3 cut(s) 81, 1007, 1457
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 5 cut(s) 84, 1184, 1289, 1374, 1705
HphI GGTGA 7 cut(s) 560, 648, 757, 841, 1099, 1388, 1564
Hpy166II GTNNAC 6 cut(s) 436, 1076, 1300, 1427, 1579, 1864
Hpy188I TCNGA 7 cut(s) 33, 606, 1276, 1395, 1474, 1816, 1885
Hpy188III TCNNGA 8 cut(s) 374, 422, 682, 806, 855, 1065, 1162, 1366
Hpy8I GTNNAC 6 cut(s) 436, 1076, 1300, 1427, 1579, 1864
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 8 cut(s) 72, 721, 742, 803, 908, 1028, 1159, 1733
HpyCH4III ACNGT 6 cut(s) 10, 219, 399, 769, 1431, 1609
HpyCH4IV ACGT 1 cut(s) 504
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 99, 123, 155, 255, 308, 1349
HpyF3I CTNAG 4 cut(s) 30, 940, 952, 1884
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 1 cut(s) 1181
Hsp92II CATG 9 cut(s) 685, 784, 850, 877, 1127, 1165, 1270, 1585, 1855
HspAI GCGC 3 cut(s) 81, 1007, 1457
KroI GCCGGC 1 cut(s) 1704
KroNI GCCGGC 1 cut(s) 1706
Ksp22I TGATCA 2 cut(s) 579, 1345
KspAI GTTAAC 1 cut(s) 436
Kzo9I GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
LmnI GCTCC 3 cut(s) 87, 948, 1777
Lsp1109I GCAGC 4 cut(s) 77, 136, 1268, 1306
LweI GCATC 4 cut(s) 909, 1129, 1177, 1654
MaeI CTAG 3 cut(s) 713, 1046, 1897
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 4 cut(s) 667, 999, 1087, 1616
MalI GATC 6 cut(s) 581, 738, 791, 1160, 1203, 1347
MboI GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
MboII GAAGA 5 cut(s) 213, 481, 1168, 1354, 1829
MhlI GDGCHC 2 cut(s) 1406, 1429
MluCI AATT 7 cut(s) 493, 754, 816, 1435, 1491, 1516, 1743
MluI ACGCGT 1 cut(s) 1649
MlyI GAGTC 2 cut(s) 652, 700
MmeI TCCRAC 2 cut(s) 291, 1452
MroNI GCCGGC 1 cut(s) 1704
MseI TTAA 5 cut(s) 435, 864, 897, 1802, 1890
MslI CAYNNNNRTG 1 cut(s) 1673
MspA1I CMGCKG 2 cut(s) 585, 1207
MspI CCGG 5 cut(s) 84, 1184, 1289, 1374, 1705
MspR9I CCNGG 2 cut(s) 85, 1374
Mva1269I GAATGC 2 cut(s) 102, 1217
MvnI CGCG 1 cut(s) 1651
MwoI GCNNNNNNNGC 7 cut(s) 87, 99, 123, 155, 255, 308, 1349
NaeI GCCGGC 1 cut(s) 1706
NciI CCSGG 2 cut(s) 85, 1374
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
NgoMIV GCCGGC 1 cut(s) 1704
NlaIII CATG 9 cut(s) 685, 784, 850, 877, 1127, 1165, 1270, 1585, 1855
NlaIV GGNNCC 1 cut(s) 1703
NmuCI GTSAC 1 cut(s) 1087
NspI RCATGY 1 cut(s) 850
PagI TCATGA 2 cut(s) 681, 1161
PceI AGGCCT 1 cut(s) 451
PciI ACATGT 1 cut(s) 846
PcsI WCGNNNNNNNCGW 3 cut(s) 572, 1187, 1526
PctI GAATGC 2 cut(s) 102, 1217
PdiI GCCGGC 1 cut(s) 1706
PleI GAGTC 2 cut(s) 651, 699
PpsI GAGTC 2 cut(s) 651, 699
PpuMI RGGWCCY 1 cut(s) 381
PscI ACATGT 1 cut(s) 846
Psp124BI GAGCTC 1 cut(s) 1406
Psp5II RGGWCCY 1 cut(s) 381
PspN4I GGNNCC 1 cut(s) 1703
PspPI GGNCC 1 cut(s) 381
PspPPI RGGWCCY 1 cut(s) 381
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 384
RsaI GTAC 5 cut(s) 401, 446, 569, 627, 989
RsaNI GTAC 5 cut(s) 400, 445, 568, 626, 988
RseI CAYNNNNRTG 1 cut(s) 1673
SacI GAGCTC 1 cut(s) 1406
SaqAI TTAA 5 cut(s) 435, 864, 897, 1802, 1890
Sau3AI GATC 6 cut(s) 579, 736, 789, 1158, 1201, 1345
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 2 cut(s) 652, 700
ScrFI CCNGG 2 cut(s) 85, 1374
SduI GDGCHC 2 cut(s) 1406, 1429
SfaNI GCATC 4 cut(s) 909, 1129, 1177, 1654
SfcI CTRYAG 2 cut(s) 91, 1563
SinI GGWCC 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 1673
SmlI CTYRAG 2 cut(s) 241, 422
SmoI CTYRAG 2 cut(s) 241, 422
Sse9I AATT 7 cut(s) 493, 754, 816, 1435, 1491, 1516, 1743
SseBI AGGCCT 1 cut(s) 451
SspMI CTAG 3 cut(s) 713, 1046, 1897
SstI GAGCTC 1 cut(s) 1406
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 2 cut(s) 83, 1372
StyI CCWWGG 1 cut(s) 1123
TaaI ACNGT 6 cut(s) 10, 219, 399, 769, 1431, 1609
TaiI ACGT 1 cut(s) 507
TaqI TCGA 7 cut(s) 375, 497, 507, 792, 1064, 1365, 1662
TasI AATT 7 cut(s) 493, 754, 816, 1435, 1491, 1516, 1743
TatI WGTACW 1 cut(s) 399
TauI GCSGC 7 cut(s) 17, 129, 149, 282, 302, 305, 1685
Tru1I TTAA 5 cut(s) 435, 864, 897, 1802, 1890
Tru9I TTAA 5 cut(s) 435, 864, 897, 1802, 1890
TscAI CASTG 2 cut(s) 1429, 1434
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 4 cut(s) 90, 149, 1281, 1319
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 5 cut(s) 380, 420, 890, 1178, 1728
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 2 cut(s) 1429, 1434
VneI GTGCAC 1 cut(s) 1425
VpaK11BI GGWCC 1 cut(s) 381
XceI RCATGY 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 1578
XspI CTAG 3 cut(s) 713, 1046, 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.