FvH4_6g02600

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005029
Physical Location & Seq
Forward (+)
1496455 .. 1507942
11488 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02600.t3

Sequence Viewer

Length: 1203 bp
ATGGGGAGGGAAATTACTGGTGTGAAGCAAGAGAAGAAAAATAATGGTGTTGTAGTGGGTACCAATGGAATTTCTACCACTAAGATCCATGTTTCTCCCAAAATTTCGGAGAGAGTCAGCCGGATCCAATCCAAGGAACACAAGGTGAAGGACTCACATGAAGAGAAAGCAGCTGACGGGGAAGGTCTTGAGAAGCAAGATATACTGGCTGTTCAGAGCACGAATCTTGATGATGACTTGACCAAGGAGAATAATGAGAAACCTGGAACTCAGAAGTCAAGCGAGAATTTAAATTCCAGCTCACCAGTTTCAAAATCTGCTGCTTTTGAAAATGGGCATTCTAATCAGACTGTTTCTCATTCCAATGCTGTGGTGACTGAAAGGGCTGGTACATGTAAAGAGACTGCTAAGGTTGAAGTTGCCACTTCAGAAAACTCCAGCACTGCAGAGTCCCCTAATATAACTAAGGCATCACAGACTCCAAATGTAGAGTCACCTACTAAGAGTTCAGAGCCAAATTCACCTCGATCATCACGAAATGGCCTACAAGCTGATACTAAGAAGCACGGTGAGGAAGAAGATAATTGGTCCTTTACTTCCTCCACTGCTGCATCTATGAGAACAAATAAGTTCAAGGTTACAGTTGGACAAGCTCCTTCATTTAAATGCAGTGAACGCGCACAAAAAAGAAACGAGTACTACTCGAAGTTAAAGGAAAAACAGCAAGCTTTGGAGGCAGAGAGACTGCAGTACGAAGCGAGGACCAGGGAAGAGCAAGAAGCAGCCATAAAGCAGCTGAGGAAGTCCTTGGTGATCAAAGCTAAACCAGTACCTAGTTTCTATTATGAGCCACCTCCTCCTAAGGCTGAGCTGAAGAAGCTTCCACTTACGCGGCCCAAATCACCAAAACTGAACAGTCTAGGCAGGAGAAGGAGCTTTGGTGATGCTCTAAACACATCTCCTAACGAAAAGGGAAAAGTTTGCCCTCGAGCACATCGTCATAGTGTTGGTTGCCAGAAAGAATGCAGTACTACTCCGAAGAATAAGGTGCAGAACAGTAGTCGTAACAGTCTTAGCAATGGTAGCAGAGTCAAAGACCAATCAAAACTGGGAGAAGAAGAAATAACGGAAACAGCTCCTAAGTTCAACCAGAAAAGACATGTAGACATCAGTTTGAATCATGAACTTGAAAGCTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

401

Amino Acids

44.23

Weight (kDa)

9.42

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 220 - 292 3.9e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 2 cut(s) 678, 892
AciI CCGC 1 cut(s) 892
AclWI GGATC 3 cut(s) 79, 118, 131
AcsI RAATTY 5 cut(s) 69, 102, 286, 292, 517
AcuI CTGAAG 2 cut(s) 411, 893
AdeI CACNNNGTG 1 cut(s) 145
AfaI GTAC 6 cut(s) 61, 391, 698, 752, 831, 1030
AfiI CCNNNNNNNGG 1 cut(s) 133
AflIII ACRYGT 2 cut(s) 392, 1159
AgsI TTSAA 7 cut(s) 312, 329, 416, 634, 1147, 1177, 1190
AjnI CCWGG 2 cut(s) 262, 764
Alw21I GWGCWC 2 cut(s) 221, 994
Alw26I GTCTC 2 cut(s) 395, 736
AlwI GGATC 3 cut(s) 79, 118, 131
Ama87I CYCGRG 1 cut(s) 987
AoxI GGCC 2 cut(s) 541, 893
ApeKI GCWGC 5 cut(s) 170, 320, 608, 782, 793
ApoI RAATTY 5 cut(s) 69, 102, 286, 292, 517
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp718I GGTACC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 3 cut(s) 588, 762, 894
AsuHPI GGTGA 9 cut(s) 157, 294, 385, 486, 513, 581, 823, 894, 953
AvaI CYCGRG 1 cut(s) 987
AvaII GGWCC 2 cut(s) 588, 762
AxyI CCTNAGG 1 cut(s) 861
BamHI GGATCC 1 cut(s) 123
BanI GGYRCC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 221, 994
BbvCI CCTCAGC 1 cut(s) 797
BbvI GCAGC 5 cut(s) 182, 307, 595, 794, 805
BciT130I CCWGG 2 cut(s) 264, 766
BclI TGATCA 1 cut(s) 813
BcoDI GTCTC 2 cut(s) 395, 736
BfaI CTAG 2 cut(s) 834, 920
BfmI CTRYAG 2 cut(s) 444, 746
BisI GCNGC 6 cut(s) 171, 321, 609, 783, 794, 893
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 6 cut(s) 172, 322, 610, 784, 795, 894
BmcAI AGTACT 2 cut(s) 698, 1030
Bme1390I CCNGG 2 cut(s) 264, 766
Bme18I GGWCC 2 cut(s) 588, 762
BmeT110I CYCGRG 1 cut(s) 987
BmgT120I GGNCC 3 cut(s) 588, 762, 894
BmiI GGNNCC 2 cut(s) 61, 125
BmrFI CCNGG 2 cut(s) 264, 766
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 3 cut(s) 479, 620, 934
BmuI ACTGGG 1 cut(s) 1118
BplI GAGNNNNNCTC 2 cut(s) 686, 718
BpmI CTGGAG 1 cut(s) 421
Bpu10I CCTNAGC 2 cut(s) 408, 797
Bpu1102I GCTNAGC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 209
BsaJI CCNNGG 4 cut(s) 132, 243, 765, 807
BsaXI ACNNNNNCTCC 2 cut(s) 925, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 5 cut(s) 22, 210, 305, 827, 1113
Bse21I CCTNAGG 1 cut(s) 861
Bse3DI GCAATG 1 cut(s) 1084
BseBI CCWGG 2 cut(s) 264, 766
BseDI CCNNGG 4 cut(s) 132, 243, 765, 807
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 3 cut(s) 284, 788, 858
BseNI ACTGG 5 cut(s) 22, 210, 305, 827, 1113
BseRI GAGGAG 1 cut(s) 846
BseXI GCAGC 5 cut(s) 182, 307, 595, 794, 805
BsgI GTGCAG 1 cut(s) 1070
Bsh1236I CGCG 2 cut(s) 678, 892
BshFI GGCC 2 cut(s) 543, 895
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 2 cut(s) 221, 994
BsiHKCI CYCGRG 1 cut(s) 987
BsiSI CCGG 1 cut(s) 121
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 395, 736
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 2 cut(s) 337, 1028
BsnI GGCC 2 cut(s) 543, 895
BsoBI CYCGRG 1 cut(s) 987
Bsp1286I GDGCHC 2 cut(s) 221, 994
Bsp143I GATC 4 cut(s) 84, 123, 527, 813
Bsp1720I GCTNAGC 1 cut(s) 867
BspACI CCGC 1 cut(s) 892
BspANI GGCC 2 cut(s) 543, 895
BspCNI CTCAG 3 cut(s) 283, 789, 859
BspFNI CGCG 2 cut(s) 678, 892
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 2 cut(s) 61, 125
BspMAI CTGCAG 2 cut(s) 448, 750
BspPI GGATC 3 cut(s) 79, 118, 131
BspQI GCTCTTC 1 cut(s) 765
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 1084
BsrI ACTGG 5 cut(s) 22, 210, 305, 827, 1113
BssECI CCNNGG 4 cut(s) 132, 243, 765, 807
BssMI GATC 4 cut(s) 84, 123, 527, 813
BssT1I CCWWGG 3 cut(s) 132, 243, 807
Bst2UI CCWGG 2 cut(s) 264, 766
Bst4CI ACNGT 6 cut(s) 352, 569, 643, 917, 1058, 1070
Bst6I CTCTTC 2 cut(s) 156, 765
BstC8I GCNNGC 1 cut(s) 726
BstFNI CGCG 2 cut(s) 678, 892
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 4 cut(s) 87, 126, 530, 816
BstMAI GTCTC 2 cut(s) 395, 736
BstMBI GATC 4 cut(s) 84, 123, 527, 813
BstMWI GCNNNNNNNGC 4 cut(s) 675, 734, 877, 1083
BstNI CCWGG 2 cut(s) 264, 766
BstNSI RCATGY 2 cut(s) 396, 1163
BstSCI CCNGG 2 cut(s) 262, 764
BstSFI CTRYAG 2 cut(s) 444, 746
BstUI CGCG 2 cut(s) 678, 892
BstV1I GCAGC 5 cut(s) 182, 307, 595, 794, 805
BstX2I RGATCY 2 cut(s) 84, 123
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 84, 123
Bsu36I CCTNAGG 1 cut(s) 861
BsuRI GGCC 2 cut(s) 543, 895
BtsI GCAGTG 3 cut(s) 441, 603, 676
BtsIMutI CAGTG 3 cut(s) 441, 603, 676
Cac8I GCNNGC 1 cut(s) 726
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 1 cut(s) 680
Cfr13I GGNCC 3 cut(s) 588, 762, 894
Csp6I GTAC 6 cut(s) 60, 390, 697, 751, 830, 1029
CviAII CATG 5 cut(s) 89, 158, 393, 1160, 1181
CviQI GTAC 6 cut(s) 60, 390, 697, 751, 830, 1029
DpnI GATC 4 cut(s) 86, 125, 529, 815
DpnII GATC 4 cut(s) 84, 123, 527, 813
DraI TTTAAA 2 cut(s) 291, 664
DraIII CACNNNGTG 1 cut(s) 145
Eam1104I CTCTTC 2 cut(s) 156, 765
EarI CTCTTC 2 cut(s) 156, 765
Eco130I CCWWGG 3 cut(s) 132, 243, 807
Eco47I GGWCC 2 cut(s) 588, 762
Eco57I CTGAAG 2 cut(s) 411, 893
Eco81I CCTNAGG 1 cut(s) 861
Eco88I CYCGRG 1 cut(s) 987
EcoRII CCWGG 2 cut(s) 262, 764
EcoT14I CCWWGG 3 cut(s) 132, 243, 807
ErhI CCWWGG 3 cut(s) 132, 243, 807
FaeI CATG 5 cut(s) 92, 161, 396, 1163, 1184
FaqI GGGAC 1 cut(s) 436
FatI CATG 5 cut(s) 88, 157, 392, 1159, 1180
FbaI TGATCA 1 cut(s) 813
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 6 cut(s) 171, 321, 609, 783, 794, 893
Fsp4HI GCNGC 6 cut(s) 171, 321, 609, 783, 794, 893
FspBI CTAG 2 cut(s) 834, 920
GlaI GCGC 1 cut(s) 679
GluI GCNGC 6 cut(s) 171, 321, 609, 783, 794, 893
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 2 cut(s) 543, 895
HapII CCGG 1 cut(s) 121
HhaI GCGC 1 cut(s) 680
Hin1II CATG 5 cut(s) 92, 161, 396, 1163, 1184
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HindIII AAGCTT 3 cut(s) 726, 878, 1192
HinfI GANTC 8 cut(s) 114, 152, 223, 449, 478, 491, 1089, 1177
HpaII CCGG 1 cut(s) 121
HphI GGTGA 9 cut(s) 157, 294, 385, 486, 513, 581, 823, 894, 953
Hpy166II GTNNAC 2 cut(s) 674, 1165
Hpy188I TCNGA 7 cut(s) 109, 216, 273, 348, 430, 511, 1038
Hpy188III TCNNGA 4 cut(s) 188, 227, 534, 1181
Hpy8I GTNNAC 2 cut(s) 674, 1165
HpyAV CCTTC 4 cut(s) 142, 176, 666, 924
HpyCH4III ACNGT 6 cut(s) 352, 569, 643, 917, 1058, 1070
HpyCH4V TGCA 6 cut(s) 446, 611, 669, 748, 1026, 1051
HpyF10VI GCNNNNNNNGC 4 cut(s) 675, 734, 877, 1083
Hsp92II CATG 5 cut(s) 92, 161, 396, 1163, 1184
HspAI GCGC 1 cut(s) 678
KpnI GGTACC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 4 cut(s) 84, 123, 527, 813
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 3 cut(s) 658, 933, 1141
Lsp1109I GCAGC 5 cut(s) 182, 307, 595, 794, 805
LweI GCATC 3 cut(s) 479, 620, 934
MaeI CTAG 2 cut(s) 834, 920
MaeIII GTNAC 4 cut(s) 373, 492, 637, 1064
MalI GATC 4 cut(s) 86, 125, 529, 815
MboI GATC 4 cut(s) 84, 123, 527, 813
MboII GAAGA 9 cut(s) 46, 173, 587, 590, 782, 886, 1051, 1127, 1130
MflI RGATCY 2 cut(s) 84, 123
MhlI GDGCHC 2 cut(s) 221, 994
MluCI AATT 7 cut(s) 12, 69, 102, 286, 292, 517, 583
MlyI GAGTC 6 cut(s) 123, 146, 458, 472, 500, 1098
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 9 cut(s) 534, 565, 610, 727, 753, 792, 864, 867, 996
MseI TTAA 3 cut(s) 290, 663, 710
MslI CAYNNNNRTG 2 cut(s) 363, 664
MspA1I CMGCKG 2 cut(s) 173, 796
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 2 cut(s) 264, 766
Mva1269I GAATGC 2 cut(s) 337, 1028
MvaI CCWGG 2 cut(s) 264, 766
MvnI CGCG 2 cut(s) 678, 892
MwoI GCNNNNNNNGC 4 cut(s) 675, 734, 877, 1083
NdeII GATC 4 cut(s) 84, 123, 527, 813
NlaIII CATG 5 cut(s) 92, 161, 396, 1163, 1184
NlaIV GGNNCC 2 cut(s) 61, 125
NmuCI GTSAC 2 cut(s) 373, 492
NspI RCATGY 2 cut(s) 396, 1163
PaeR7I CTCGAG 1 cut(s) 987
PagI TCATGA 1 cut(s) 1180
PciI ACATGT 2 cut(s) 392, 1159
PciSI GCTCTTC 1 cut(s) 765
PcsI WCGNNNNNNNCGW 2 cut(s) 532, 994
PctI GAATGC 2 cut(s) 337, 1028
PfeI GAWTC 2 cut(s) 223, 1177
PkrI GCNGC 6 cut(s) 172, 322, 610, 784, 795, 894
PleI GAGTC 6 cut(s) 122, 146, 457, 472, 499, 1097
PpsI GAGTC 6 cut(s) 122, 146, 457, 472, 499, 1097
PscI ACATGT 2 cut(s) 392, 1159
Psp6I CCWGG 2 cut(s) 262, 764
PspGI CCWGG 2 cut(s) 262, 764
PspN4I GGNNCC 2 cut(s) 61, 125
PspPI GGNCC 3 cut(s) 588, 762, 894
PspXI VCTCGAGB 1 cut(s) 987
PstI CTGCAG 2 cut(s) 448, 750
PsuI RGATCY 2 cut(s) 84, 123
PvuII CAGCTG 2 cut(s) 173, 796
RsaI GTAC 6 cut(s) 61, 391, 698, 752, 831, 1030
RsaNI GTAC 6 cut(s) 60, 390, 697, 751, 830, 1029
RseI CAYNNNNRTG 2 cut(s) 363, 664
SapI GCTCTTC 1 cut(s) 765
SaqAI TTAA 3 cut(s) 290, 663, 710
SatI GCNGC 6 cut(s) 171, 321, 609, 783, 794, 893
Sau3AI GATC 4 cut(s) 84, 123, 527, 813
Sau96I GGNCC 3 cut(s) 588, 762, 894
ScaI AGTACT 2 cut(s) 698, 1030
SchI GAGTC 6 cut(s) 123, 146, 458, 472, 500, 1098
ScrFI CCNGG 2 cut(s) 264, 766
SduI GDGCHC 2 cut(s) 221, 994
SfaNI GCATC 3 cut(s) 479, 620, 934
SfcI CTRYAG 2 cut(s) 444, 746
Sfr274I CTCGAG 1 cut(s) 987
SinI GGWCC 2 cut(s) 588, 762
SlaI CTCGAG 1 cut(s) 987
SmiI ATTTAAAT 2 cut(s) 291, 664
SmiMI CAYNNNNRTG 2 cut(s) 363, 664
SmlI CTYRAG 2 cut(s) 188, 987
SmoI CTYRAG 2 cut(s) 188, 987
Sse9I AATT 7 cut(s) 12, 69, 102, 286, 292, 517, 583
SsiI CCGC 1 cut(s) 892
SspMI CTAG 2 cut(s) 834, 920
StyD4I CCNGG 2 cut(s) 262, 764
StyI CCWWGG 3 cut(s) 132, 243, 807
SwaI ATTTAAAT 2 cut(s) 291, 664
TaaI ACNGT 6 cut(s) 352, 569, 643, 917, 1058, 1070
TaqI TCGA 3 cut(s) 526, 704, 988
TasI AATT 7 cut(s) 12, 69, 102, 286, 292, 517, 583
TatI WGTACW 2 cut(s) 696, 1028
TauI GCSGC 1 cut(s) 895
TfiI GAWTC 2 cut(s) 223, 1177
Tru1I TTAA 3 cut(s) 290, 663, 710
Tru9I TTAA 3 cut(s) 290, 663, 710
TscAI CASTG 3 cut(s) 448, 610, 676
TseFI GTSAC 2 cut(s) 373, 492
TseI GCWGC 5 cut(s) 170, 320, 608, 782, 793
Tsp45I GTSAC 2 cut(s) 373, 492
TspDTI ATGAA 3 cut(s) 174, 648, 1197
TspGWI ACGGA 1 cut(s) 1142
TspRI CASTG 3 cut(s) 448, 610, 676
VpaK11BI GGWCC 2 cut(s) 588, 762
XapI RAATTY 5 cut(s) 69, 102, 286, 292, 517
XceI RCATGY 2 cut(s) 396, 1163
XhoI CTCGAG 1 cut(s) 987
XmiI GTMKAC 1 cut(s) 1164
XspI CTAG 2 cut(s) 834, 920
ZrmI AGTACT 2 cut(s) 698, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.