Rh3AG029000

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005029
Physical Location & Seq
Forward (+)
1951346 .. 1956346
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG029000.1

Sequence Viewer

Length: 945 bp
ATGGGGAGGGAAATTACTGGTATGAAGGAAGAGAAGAAAATTAATGGTGTTGTAGTGGGTGCCAATGGTTTTTCTACTGCTAAACTCCATGTCTCTCCCAAAATTTCCGAGAGAATCAGTAGGATCGAGTCCAAGGAGTACAAGGTGAAGGACTCTAATGAAGAGAAAGCAGCTGAGGGGGAAGGTCTTGAGAAGCAAGATATGCTGGCTGTTAAGAGCACGAATCTTGATGATGACTTGACCAAGGAGAATAATGAGAAACCTGAAACTCAGAAGTCAAGCGAGAGTTTAAATTCCAGCTCACCAGTTTCAAAATCTGCTGCTGTTGAAAATGGGCACTCAAATCAGACTCTTCCTCATCCCAATGCTCTGGTAACTGAAAGGGCTGGTACATGTGAAGAGACTGCTGGGGCTGAAGTTGCCACTTCAGAAAACTCCAGCACTGCAGAGTCTCCTAATGCAACTAAGACTTCACAGACTGCCAATGCAGAGTCACCTACTAAGAGTTCAGAGCCAAATTCACCTCGGTCATCACGACCTGATACTAAGAAGCATGCTGGGGAAGAAGATAATTGGTCTCTTACTTCCTCAACTGCTGCATCTGTGAGAACAAATAAATTCAAGGTTACAGTTGGACAAGCTCCTTCGTTTAAATGCAGTGAACGCGCACAAAAAAGAAATGAGTACTACTCAAAGTTAAAGGAAAAACAAGAAGCTCTGGAGGCAGAGAGATTGCAGTACGAATCAAGGACCAGGGAAGAGCAAGAAGCAGCCATCAAGCAGCTGAGGAAGTCCTTGGTGATCAAAGCAAAGCCAGTACCTAGTTTCTATTATGAGCCACCTCCTCCTAAGGCTGAACTCAAGAAGGTAAGTAGCCAAAATCATTTCGAGTTGTGTTCGACTCTTTCTAAGTTCTATTCTGATGCCCAGACTCTAGATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

314

Amino Acids

34.49

Weight (kDa)

6.59

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 216 - 285 1.9e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccII CGCG 1 cut(s) 666
AclWI GGATC 1 cut(s) 131
AcsI RAATTY 4 cut(s) 102, 292, 517, 617
AcuI CTGAAG 2 cut(s) 411, 435
AfaI GTAC 5 cut(s) 140, 391, 686, 740, 819
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 3 cut(s) 312, 329, 622
AjnI CCWGG 1 cut(s) 752
AluBI AGCT 5 cut(s) 173, 300, 641, 716, 784
AluI AGCT 5 cut(s) 173, 300, 641, 716, 784
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 4 cut(s) 97, 395, 456, 582
AlwI GGATC 1 cut(s) 131
ApeKI GCWGC 5 cut(s) 170, 320, 596, 770, 781
ApoI RAATTY 4 cut(s) 102, 292, 517, 617
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 1 cut(s) 750
AsuHPI GGTGA 5 cut(s) 157, 294, 486, 513, 811
AvaII GGWCC 1 cut(s) 750
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 2 cut(s) 174, 785
BbvI GCAGC 5 cut(s) 182, 307, 583, 782, 793
BccI CCATC 1 cut(s) 782
BciT130I CCWGG 1 cut(s) 754
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 4 cut(s) 97, 395, 456, 582
BfaI CTAG 2 cut(s) 822, 935
BfmI CTRYAG 1 cut(s) 444
BisI GCNGC 5 cut(s) 171, 321, 597, 771, 782
BlsI GCNGC 5 cut(s) 172, 322, 598, 772, 783
BmcAI AGTACT 1 cut(s) 686
Bme1390I CCNGG 1 cut(s) 754
Bme18I GGWCC 1 cut(s) 750
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 608, 913
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 2 cut(s) 421, 740
Bpu10I CCTNAGC 2 cut(s) 174, 785
BpuEI CTTGAG 2 cut(s) 209, 845
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
Bse1I ACTGG 3 cut(s) 22, 305, 815
Bse21I CCTNAGG 1 cut(s) 849
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
BseGI GGATG 1 cut(s) 358
BseMII CTCAG 3 cut(s) 165, 284, 776
BseNI ACTGG 3 cut(s) 22, 305, 815
BseRI GAGGAG 1 cut(s) 834
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 5 cut(s) 182, 307, 583, 782, 793
BseYI CCCAGC 2 cut(s) 407, 557
Bsh1236I CGCG 1 cut(s) 666
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 221
BsmAI GTCTC 4 cut(s) 97, 395, 456, 582
Bso31I GGTCTC 1 cut(s) 582
Bsp1286I GDGCHC 2 cut(s) 221, 339
Bsp143I GATC 2 cut(s) 123, 801
BspCNI CTCAG 3 cut(s) 166, 283, 777
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 582
BsrI ACTGG 3 cut(s) 22, 305, 815
BssECI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
BssMI GATC 2 cut(s) 123, 801
BssT1I CCWWGG 3 cut(s) 132, 243, 795
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 1 cut(s) 631
Bst6I CTCTTC 5 cut(s) 24, 156, 357, 393, 753
BstAPI GCANNNNNTGC 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 207, 555
BstDEI CTNAG 8 cut(s) 174, 270, 465, 501, 546, 785, 849, 909
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 666
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 2 cut(s) 126, 804
BstMAI GTCTC 4 cut(s) 97, 395, 456, 582
BstMBI GATC 2 cut(s) 123, 801
BstMWI GCNNNNNNNGC 4 cut(s) 202, 419, 663, 722
BstNI CCWGG 1 cut(s) 754
BstNSI RCATGY 2 cut(s) 396, 557
BstSCI CCNGG 1 cut(s) 752
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 5 cut(s) 182, 307, 583, 782, 793
BstXI CCANNNNNNTGG 1 cut(s) 370
Bsu36I CCTNAGG 1 cut(s) 849
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 2 cut(s) 441, 664
BtsIMutI CAGTG 2 cut(s) 441, 664
Cac8I GCNNGC 2 cut(s) 207, 555
CfoI GCGC 1 cut(s) 668
Cfr13I GGNCC 1 cut(s) 750
Csp6I GTAC 5 cut(s) 139, 390, 685, 739, 818
CviAII CATG 3 cut(s) 89, 393, 554
CviQI GTAC 5 cut(s) 139, 390, 685, 739, 818
DdeI CTNAG 8 cut(s) 174, 270, 465, 501, 546, 785, 849, 909
DpnI GATC 2 cut(s) 125, 803
DpnII GATC 2 cut(s) 123, 801
DraI TTTAAA 2 cut(s) 291, 652
Eam1104I CTCTTC 5 cut(s) 24, 156, 357, 393, 753
EarI CTCTTC 5 cut(s) 24, 156, 357, 393, 753
Eco130I CCWWGG 3 cut(s) 132, 243, 795
Eco31I GGTCTC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 750
Eco57I CTGAAG 2 cut(s) 411, 435
Eco81I CCTNAGG 1 cut(s) 849
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 3 cut(s) 132, 243, 795
ErhI CCWWGG 3 cut(s) 132, 243, 795
FaeI CATG 3 cut(s) 92, 396, 557
FaiI YATR 6 cut(s) 23, 90, 203, 394, 555, 834
FatI CATG 3 cut(s) 88, 392, 553
FbaI TGATCA 1 cut(s) 801
Fnu4HI GCNGC 5 cut(s) 171, 321, 597, 771, 782
FokI GGATG 1 cut(s) 345
Fsp4HI GCNGC 5 cut(s) 171, 321, 597, 771, 782
FspBI CTAG 2 cut(s) 822, 935
GlaI GCGC 1 cut(s) 667
GluI GCNGC 5 cut(s) 171, 321, 597, 771, 782
GsaI CCCAGC 2 cut(s) 411, 561
GsuI CTGGAG 2 cut(s) 421, 740
HhaI GCGC 1 cut(s) 668
Hin1II CATG 3 cut(s) 92, 396, 557
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HphI GGTGA 5 cut(s) 157, 294, 486, 513, 811
Hpy166II GTNNAC 1 cut(s) 662
Hpy188I TCNGA 6 cut(s) 109, 273, 348, 430, 511, 922
Hpy188III TCNNGA 6 cut(s) 188, 227, 534, 719, 862, 935
Hpy8I GTNNAC 1 cut(s) 662
HpyAV CCTTC 5 cut(s) 19, 142, 176, 654, 859
HpyCH4III ACNGT 1 cut(s) 631
HpyCH4V TGCA 6 cut(s) 446, 461, 488, 599, 657, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 202, 419, 663, 722
HpyF3I CTNAG 8 cut(s) 174, 270, 465, 501, 546, 785, 849, 909
Hsp92II CATG 3 cut(s) 92, 396, 557
HspAI GCGC 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 2 cut(s) 123, 801
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 5 cut(s) 182, 307, 583, 782, 793
LweI GCATC 2 cut(s) 608, 913
MaeI CTAG 2 cut(s) 822, 935
MaeIII GTNAC 3 cut(s) 373, 492, 625
MalI GATC 2 cut(s) 125, 803
MboI GATC 2 cut(s) 123, 801
MboII GAAGA 8 cut(s) 41, 46, 173, 344, 410, 575, 578, 770
MhlI GDGCHC 2 cut(s) 221, 339
MluCI AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
MlyI GAGTC 7 cut(s) 137, 146, 343, 458, 500, 895, 925
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 8 cut(s) 169, 366, 534, 598, 715, 780, 852, 855
MseI TTAA 6 cut(s) 42, 213, 290, 651, 698, 943
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 173, 784
MspR9I CCNGG 1 cut(s) 754
MvaI CCWGG 1 cut(s) 754
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 4 cut(s) 202, 419, 663, 722
NdeII GATC 2 cut(s) 123, 801
NlaIII CATG 3 cut(s) 92, 396, 557
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 492
NspI RCATGY 2 cut(s) 396, 557
PaeI GCATGC 1 cut(s) 557
PciI ACATGT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 3 cut(s) 114, 223, 743
PkrI GCNGC 5 cut(s) 172, 322, 598, 772, 783
PleI GAGTC 7 cut(s) 136, 146, 343, 457, 499, 895, 925
PpsI GAGTC 7 cut(s) 136, 146, 343, 457, 499, 895, 925
PscI ACATGT 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 752
PspFI CCCAGC 2 cut(s) 407, 557
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 1 cut(s) 750
PstI CTGCAG 1 cut(s) 448
PvuII CAGCTG 2 cut(s) 173, 784
RsaI GTAC 5 cut(s) 140, 391, 686, 740, 819
RsaNI GTAC 5 cut(s) 139, 390, 685, 739, 818
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 6 cut(s) 42, 213, 290, 651, 698, 943
SatI GCNGC 5 cut(s) 171, 321, 597, 771, 782
Sau3AI GATC 2 cut(s) 123, 801
Sau96I GGNCC 1 cut(s) 750
ScaI AGTACT 1 cut(s) 686
SchI GAGTC 7 cut(s) 137, 146, 343, 458, 500, 895, 925
ScrFI CCNGG 1 cut(s) 754
SduI GDGCHC 2 cut(s) 221, 339
SfaNI GCATC 2 cut(s) 608, 913
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 2 cut(s) 188, 860
SmoI CTYRAG 2 cut(s) 188, 860
SphI GCATGC 1 cut(s) 557
Sse9I AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
SspMI CTAG 2 cut(s) 822, 935
StyD4I CCNGG 1 cut(s) 752
StyI CCWWGG 3 cut(s) 132, 243, 795
TaaI ACNGT 1 cut(s) 631
TaqI TCGA 3 cut(s) 126, 888, 899
TaqII GACCGA 1 cut(s) 516
TasI AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
TatI WGTACW 2 cut(s) 138, 684
TfiI GAWTC 3 cut(s) 114, 223, 743
Tru1I TTAA 6 cut(s) 42, 213, 290, 651, 698, 943
Tru9I TTAA 6 cut(s) 42, 213, 290, 651, 698, 943
TscAI CASTG 2 cut(s) 448, 664
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 5 cut(s) 170, 320, 596, 770, 781
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 2 cut(s) 38, 174
TspRI CASTG 2 cut(s) 448, 664
VpaK11BI GGWCC 1 cut(s) 750
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 4 cut(s) 102, 292, 517, 617
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 2 cut(s) 396, 557
XspI CTAG 2 cut(s) 822, 935
ZrmI AGTACT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.