Rw3G002240

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005029
Physical Location & Seq
Forward (+)
1975335 .. 1979935
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G002240.1

Sequence Viewer

Length: 1197 bp
ATGGGGAGGGAAATTACTGGTATGAAGGAAGAGAAGAAAATTAATGGTGTTGTAGTGGGTACCAATGGTTTTTCTACTGCTAAACTCCATGTCTCTCCCAAAATTTCCGAGAGAATCAGCAGGATCGAGTCCAAGGAGTACAAGGTGAAGGACTCTAATGAAGAGAAAGCAGCTGAGGGGGAAGGTCTTGAGAAGCAAGATATGCTGGCTGTTAAGACCACGAATCTTGATGATGACTTGACCAAGGAGAATAATGAGAAACCTGAAACTCAGAAGTCAAGCGAGAGTTTAAATTCCAGCTCACCAGTTTCAAAATCTGCTGCTGTTGAAAATGGGCACTCAAATCAGACTGTTCCTCATCCCAATGCTCTGGTAACTGAAAGGGCTGGTACATGTGAAGAGACTGCTGGGGCTGAAGTTGCCACTTCAGAAAACTCCAGCACTGCAGAGTCTCCTAATGCAACTAAGACTTCACAGACTGCCAATGCAGAGTCACCTACTAAGAGTTCAGAGCCAAATTCACCTCGGTCATCACGACCTGATACTAAGAAGCATGCTGAGGAAGAAGATAATTGGTCTCTTACTTCCTCAACTGCTGCATCTGTGAGAACAAATAAATTCAAGGTTACAGTTGGACAAGCTCCTTCGTTTAAATGCAGTGAACGCGCACAAAAAAGAAATGAGTACTACTCGAAGTTAAAGGAAAAACAAGAAGCTCTGGAGGCAGAGAGATTGCAGTACGAATCAAGGACCAGGGAAGAGCAAGAAGCAGCCATCAAGCAGCTGAGGAAGTCCTTGGTGATCAAAGCAAAGCCAGTACCTAGTTTCTATTATGAGCCACCTCCTCCTAAGGCTGAACTCAAGAAGCTTCCACTTACGCGGCCCAAGTCACCAAAACTGAACAGTCTAAGCAGGAGAAAGAGCTTTGGTGATGCTCTCAACACATCTCATGACGAAAAGGGAAGAGTTTGTCCTCGAGCACATCGTCACAGCGTTGGTTGCCAGAAAGAATGCACTGCATCGACTACTCCAAAGAATAAGGTGCAGAACAGTAGACGTAACAGTCTTGGCAATGGTAGCAAAGTCAAAGACCAATCAAAACAGGGAGAAGAAGAAACAACGAAAACAGCTCCTAAGTTCACCCAGAAGAGAAACGTAGACATCAGTTTGAATCATGAACTCGAAAGCTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

398

Amino Acids

43.99

Weight (kDa)

9.19

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 216 - 288 2.1e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1062
Acc65I GGTACC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 2 cut(s) 1054, 1158
AccII CGCG 2 cut(s) 666, 880
AciI CCGC 1 cut(s) 880
AclWI GGATC 1 cut(s) 131
AcsI RAATTY 4 cut(s) 102, 292, 517, 617
AcuI CTGAAG 2 cut(s) 411, 435
AfaI GTAC 6 cut(s) 61, 140, 391, 686, 740, 819
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 4 cut(s) 312, 329, 622, 1171
AjnI CCWGG 1 cut(s) 752
AluBI AGCT 9 cut(s) 173, 300, 641, 716, 784, 868, 924, 1130, 1188
AluI AGCT 9 cut(s) 173, 300, 641, 716, 784, 868, 924, 1130, 1188
Alw21I GWGCWC 1 cut(s) 982
Alw26I GTCTC 4 cut(s) 97, 395, 456, 582
AlwI GGATC 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 975
AoxI GGCC 1 cut(s) 881
ApeKI GCWGC 5 cut(s) 170, 320, 596, 770, 781
ApoI RAATTY 4 cut(s) 102, 292, 517, 617
AseI ATTAAT 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 2 cut(s) 750, 882
AsuHPI GGTGA 8 cut(s) 157, 294, 486, 513, 811, 882, 941, 1132
AvaI CYCGRG 1 cut(s) 975
AvaII GGWCC 1 cut(s) 750
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 982
BbvCI CCTCAGC 3 cut(s) 174, 558, 785
BbvI GCAGC 5 cut(s) 182, 307, 583, 782, 793
BccI CCATC 1 cut(s) 782
BciT130I CCWGG 1 cut(s) 754
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 4 cut(s) 97, 395, 456, 582
BfaI CTAG 1 cut(s) 822
BfmI CTRYAG 1 cut(s) 444
BisI GCNGC 6 cut(s) 171, 321, 597, 771, 782, 881
BlsI GCNGC 6 cut(s) 172, 322, 598, 772, 783, 882
BmcAI AGTACT 1 cut(s) 686
Bme1390I CCNGG 1 cut(s) 754
Bme18I GGWCC 1 cut(s) 750
BmeT110I CYCGRG 1 cut(s) 975
BmgT120I GGNCC 2 cut(s) 750, 882
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 754
BmsI GCATC 3 cut(s) 608, 922, 1028
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 2 cut(s) 421, 740
Bpu10I CCTNAGC 3 cut(s) 174, 558, 785
BpuEI CTTGAG 2 cut(s) 209, 845
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
Bse1I ACTGG 3 cut(s) 22, 305, 815
Bse21I CCTNAGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 1078
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
BseGI GGATG 1 cut(s) 358
BseMI GCAATG 1 cut(s) 1078
BseMII CTCAG 4 cut(s) 165, 284, 549, 776
BseNI ACTGG 3 cut(s) 22, 305, 815
BseRI GAGGAG 1 cut(s) 834
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 5 cut(s) 182, 307, 583, 782, 793
BseYI CCCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 1064
Bsh1236I CGCG 2 cut(s) 666, 880
BshFI GGCC 1 cut(s) 883
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 982
BsiHKCI CYCGRG 1 cut(s) 975
BsmAI GTCTC 4 cut(s) 97, 395, 456, 582
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 1 cut(s) 883
Bso31I GGTCTC 1 cut(s) 582
BsoBI CYCGRG 1 cut(s) 975
Bsp1286I GDGCHC 2 cut(s) 339, 982
Bsp143I GATC 2 cut(s) 123, 801
BspACI CCGC 1 cut(s) 880
BspANI GGCC 1 cut(s) 883
BspCNI CTCAG 4 cut(s) 166, 283, 550, 777
BspFNI CGCG 2 cut(s) 666, 880
BspHI TCATGA 2 cut(s) 949, 1174
BspLI GGNNCC 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 1078
BsrI ACTGG 3 cut(s) 22, 305, 815
BssECI CCNNGG 5 cut(s) 132, 243, 524, 753, 795
BssMI GATC 2 cut(s) 123, 801
BssT1I CCWWGG 3 cut(s) 132, 243, 795
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 5 cut(s) 352, 631, 905, 1052, 1064
Bst6I CTCTTC 6 cut(s) 24, 156, 393, 753, 958, 1142
BstAPI GCANNNNNTGC 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 207, 555
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 2 cut(s) 666, 880
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 2 cut(s) 126, 804
BstMAI GTCTC 4 cut(s) 97, 395, 456, 582
BstMBI GATC 2 cut(s) 123, 801
BstMWI GCNNNNNNNGC 6 cut(s) 202, 419, 663, 722, 999, 1077
BstNI CCWGG 1 cut(s) 754
BstNSI RCATGY 2 cut(s) 396, 557
BstSCI CCNGG 1 cut(s) 752
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 2 cut(s) 666, 880
BstV1I GCAGC 5 cut(s) 182, 307, 583, 782, 793
BstXI CCANNNNNNTGG 1 cut(s) 370
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 1 cut(s) 883
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 3 cut(s) 441, 664, 1014
BtsIMutI CAGTG 3 cut(s) 441, 664, 1014
Cac8I GCNNGC 2 cut(s) 207, 555
CciI TCATGA 2 cut(s) 949, 1174
CfoI GCGC 1 cut(s) 668
Cfr13I GGNCC 2 cut(s) 750, 882
Csp6I GTAC 6 cut(s) 60, 139, 390, 685, 739, 818
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 5 cut(s) 89, 393, 554, 950, 1175
CviQI GTAC 6 cut(s) 60, 139, 390, 685, 739, 818
DpnI GATC 2 cut(s) 125, 803
DpnII GATC 2 cut(s) 123, 801
DraI TTTAAA 2 cut(s) 291, 652
DrdI GACNNNNNNGTC 1 cut(s) 1062
DseDI GACNNNNNNGTC 1 cut(s) 1062
Eam1104I CTCTTC 6 cut(s) 24, 156, 393, 753, 958, 1142
EarI CTCTTC 6 cut(s) 24, 156, 393, 753, 958, 1142
Eco130I CCWWGG 3 cut(s) 132, 243, 795
Eco31I GGTCTC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 750
Eco57I CTGAAG 2 cut(s) 411, 435
Eco81I CCTNAGG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 975
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 3 cut(s) 132, 243, 795
ErhI CCWWGG 3 cut(s) 132, 243, 795
FaeI CATG 5 cut(s) 92, 396, 557, 953, 1178
FaiI YATR 8 cut(s) 23, 90, 203, 394, 555, 834, 951, 1176
FatI CATG 5 cut(s) 88, 392, 553, 949, 1174
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 2 cut(s) 1054, 1158
Fnu4HI GCNGC 6 cut(s) 171, 321, 597, 771, 782, 881
FokI GGATG 1 cut(s) 345
Fsp4HI GCNGC 6 cut(s) 171, 321, 597, 771, 782, 881
FspBI CTAG 1 cut(s) 822
GlaI GCGC 1 cut(s) 667
GluI GCNGC 6 cut(s) 171, 321, 597, 771, 782, 881
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 421, 740
HaeIII GGCC 1 cut(s) 883
HhaI GCGC 1 cut(s) 668
Hin1II CATG 5 cut(s) 92, 396, 557, 953, 1178
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 2 cut(s) 866, 1186
HinfI GANTC 8 cut(s) 114, 128, 152, 223, 449, 491, 743, 1171
HphI GGTGA 8 cut(s) 157, 294, 486, 513, 811, 882, 941, 1132
Hpy166II GTNNAC 4 cut(s) 662, 1055, 1140, 1159
Hpy188I TCNGA 5 cut(s) 109, 273, 348, 430, 511
Hpy188III TCNNGA 7 cut(s) 188, 227, 534, 719, 862, 950, 1175
Hpy8I GTNNAC 4 cut(s) 662, 1055, 1140, 1159
HpyAV CCTTC 4 cut(s) 19, 142, 176, 654
HpyCH4III ACNGT 5 cut(s) 352, 631, 905, 1052, 1064
HpyCH4IV ACGT 2 cut(s) 1057, 1155
HpyCH4V TGCA 9 cut(s) 446, 461, 488, 599, 657, 736, 1014, 1019, 1045
HpyF10VI GCNNNNNNNGC 6 cut(s) 202, 419, 663, 722, 999, 1077
HpySE526I ACGT 2 cut(s) 1057, 1155
Hsp92II CATG 5 cut(s) 92, 396, 557, 953, 1178
HspAI GCGC 1 cut(s) 666
KpnI GGTACC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 2 cut(s) 123, 801
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 2 cut(s) 646, 1135
Lsp1109I GCAGC 5 cut(s) 182, 307, 583, 782, 793
LweI GCATC 3 cut(s) 608, 922, 1028
MaeI CTAG 1 cut(s) 822
MaeII ACGT 2 cut(s) 1057, 1155
MaeIII GTNAC 6 cut(s) 373, 492, 625, 888, 986, 1058
MalI GATC 2 cut(s) 125, 803
MboI GATC 2 cut(s) 123, 801
MhlI GDGCHC 2 cut(s) 339, 982
MluCI AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
MlyI GAGTC 4 cut(s) 137, 146, 458, 500
MmeI TCCRAC 1 cut(s) 613
MseI TTAA 5 cut(s) 42, 213, 290, 651, 698
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 173, 784
MspR9I CCNGG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 1 cut(s) 754
MvnI CGCG 2 cut(s) 666, 880
MwoI GCNNNNNNNGC 6 cut(s) 202, 419, 663, 722, 999, 1077
NdeII GATC 2 cut(s) 123, 801
NlaIII CATG 5 cut(s) 92, 396, 557, 953, 1178
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 3 cut(s) 492, 888, 986
NspI RCATGY 2 cut(s) 396, 557
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 975
PagI TCATGA 2 cut(s) 949, 1174
PciI ACATGT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 532, 982
PctI GAATGC 1 cut(s) 1016
PfeI GAWTC 4 cut(s) 114, 223, 743, 1171
PkrI GCNGC 6 cut(s) 172, 322, 598, 772, 783, 882
PleI GAGTC 4 cut(s) 136, 146, 457, 499
PpsI GAGTC 4 cut(s) 136, 146, 457, 499
PscI ACATGT 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 752
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 750, 882
PspXI VCTCGAGB 1 cut(s) 975
PstI CTGCAG 1 cut(s) 448
PvuII CAGCTG 2 cut(s) 173, 784
RsaI GTAC 6 cut(s) 61, 140, 391, 686, 740, 819
RsaNI GTAC 6 cut(s) 60, 139, 390, 685, 739, 818
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 5 cut(s) 42, 213, 290, 651, 698
SatI GCNGC 6 cut(s) 171, 321, 597, 771, 782, 881
Sau3AI GATC 2 cut(s) 123, 801
Sau96I GGNCC 2 cut(s) 750, 882
ScaI AGTACT 1 cut(s) 686
SchI GAGTC 4 cut(s) 137, 146, 458, 500
ScrFI CCNGG 1 cut(s) 754
SduI GDGCHC 2 cut(s) 339, 982
SfaNI GCATC 3 cut(s) 608, 922, 1028
SfcI CTRYAG 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 975
SinI GGWCC 1 cut(s) 750
SlaI CTCGAG 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 3 cut(s) 188, 860, 975
SmoI CTYRAG 3 cut(s) 188, 860, 975
SphI GCATGC 1 cut(s) 557
Sse9I AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
SsiI CCGC 1 cut(s) 880
SspMI CTAG 1 cut(s) 822
StyD4I CCNGG 1 cut(s) 752
StyI CCWWGG 3 cut(s) 132, 243, 795
TaaI ACNGT 5 cut(s) 352, 631, 905, 1052, 1064
TaiI ACGT 2 cut(s) 1060, 1158
TaqI TCGA 5 cut(s) 126, 692, 976, 1022, 1182
TaqII GACCGA 1 cut(s) 516
TasI AATT 7 cut(s) 12, 39, 102, 292, 517, 571, 617
TatI WGTACW 2 cut(s) 138, 684
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 4 cut(s) 114, 223, 743, 1171
Tru1I TTAA 5 cut(s) 42, 213, 290, 651, 698
Tru9I TTAA 5 cut(s) 42, 213, 290, 651, 698
TscAI CASTG 3 cut(s) 448, 664, 1021
TseFI GTSAC 3 cut(s) 492, 888, 986
TseI GCWGC 5 cut(s) 170, 320, 596, 770, 781
Tsp45I GTSAC 3 cut(s) 492, 888, 986
TspDTI ATGAA 3 cut(s) 38, 174, 1191
TspRI CASTG 3 cut(s) 448, 664, 1021
VpaK11BI GGWCC 1 cut(s) 750
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 4 cut(s) 102, 292, 517, 617
XceI RCATGY 2 cut(s) 396, 557
XhoI CTCGAG 1 cut(s) 975
XmiI GTMKAC 2 cut(s) 1054, 1158
XspI CTAG 1 cut(s) 822
ZrmI AGTACT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.