Rorug02G0628400

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000230
Physical Location & Seq
Forward (+)
74351269 .. 74354786
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0628400.1

Sequence Viewer

Length: 804 bp
ATGGCGACGAGTGAGGAGAAGACCAACAATACTAAAGAAGGGAACAACCTACTGGGCTCGCCGACCTTCAAGGAGCTCGATAATGGTCGCTTCCAGTGCGTCGAGACTGGCCACCAATTACTGGCCAAAGACATTGAATCCTACTCTCAGAGCAAACGGTGCCGTTTGGGTCTCATCGAATTCGCACTCTCTCATAAGAAGCCTCCTCTCAACATGTTCAAGCAGGACCCTCTTTCTCGTTCAAAGTTGGTGTGTAAGCTAACCGAGGATACCATCAATAAGTCAGAGGAACACATTTGGAAGCATATTAATGGGAAACGATTCCTCAACAAACTGGAAGAAATGGAAGAACAAAAGCTAACAGTAAATGAAATGGAAGAAGAGCTGGATGATCACAAACCTGAAAAAGTATCTAAGCCAGGCAAAGATGGTGCCAGGAAGGAGAAAAAGGAGAAGAAAAAGAAGAAGAAGAAGGAGGAGGCAAAGGAGGAGGATGATGGTGATGATGAGATTATCTCTGAAATACCCAAGTCTCCTAGTGAGGAAAATGATTCTGAAGAAGCTGAGTTTTGGATCCCTCCACCAGGAGCACGTTGGGATTTTGATGATGGCGGTGACAGATGGGGTTCCGATTCAGAGTCAGAGAGCAATGACGAGAACAAAAGTGGCGAGGTTGATGGAATTGTTGAAGATGATGATGAGGAGTCAGAAGAGATCTCAATGCGGACAAAAAGAATGTCCATAGAAATTGGACCAAGCAGCTTTGCATCCAGAAAGAAGAAGAGTAAAAAGAACACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

267

Amino Acids

30.54

Weight (kDa)

5.05

Isoelectric Point (pI)

68.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF2 PF05477 12 - 263 9.2e-58 Surfeit locus protein 2 (SURF2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 159, 431
AccB7I CCANNNNNTGG 1 cut(s) 121
AciI CCGC 2 cut(s) 612, 724
AclWI GGATC 2 cut(s) 568, 581
AcoI YGGCCR 2 cut(s) 109, 123
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 576
AfiI CCNNNNNNNGG 2 cut(s) 121, 584
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 5 cut(s) 70, 137, 220, 243, 689
AjnI CCWGG 3 cut(s) 418, 434, 583
AluBI AGCT 6 cut(s) 76, 259, 358, 385, 563, 762
AluI AGCT 6 cut(s) 76, 259, 358, 385, 563, 762
Alw21I GWGCWC 2 cut(s) 78, 592
Alw26I GTCTC 3 cut(s) 98, 176, 537
AlwI GGATC 2 cut(s) 568, 581
AoxI GGCC 2 cut(s) 109, 123
ApeKI GCWGC 1 cut(s) 759
ApoI RAATTY 1 cut(s) 179
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AseI ATTAAT 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 226, 752
AsuHPI GGTGA 2 cut(s) 512, 626
AvaII GGWCC 2 cut(s) 226, 752
BalI TGGCCA 2 cut(s) 111, 125
BamHI GGATCC 1 cut(s) 573
BanI GGYRCC 2 cut(s) 159, 431
BanII GRGCYC 2 cut(s) 59, 78
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 78, 592
BbvI GCAGC 1 cut(s) 771
BccI CCATC 6 cut(s) 281, 422, 491, 602, 615, 671
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 3 cut(s) 420, 436, 585
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 3 cut(s) 98, 176, 537
BfaI CTAG 1 cut(s) 537
BfuI GTATCC 1 cut(s) 262
BglII AGATCT 1 cut(s) 714
BisI GCNGC 1 cut(s) 760
BlsI GCNGC 1 cut(s) 761
Bme1390I CCNGG 3 cut(s) 420, 436, 585
Bme18I GGWCC 2 cut(s) 226, 752
BmgT120I GGNCC 2 cut(s) 226, 752
BmiI GGNNCC 5 cut(s) 161, 228, 433, 575, 628
BmrFI CCNGG 3 cut(s) 420, 436, 585
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 776
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 26
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 264
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 584
Bse1I ACTGG 5 cut(s) 57, 94, 112, 126, 339
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 3 cut(s) 420, 436, 585
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 3 cut(s) 394, 499, 767
BseLI CCNNNNNNNGG 2 cut(s) 121, 584
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 2 cut(s) 161, 555
BseNI ACTGG 5 cut(s) 57, 94, 112, 126, 339
BseRI GAGGAG 5 cut(s) 29, 195, 491, 503, 716
BseXI GCAGC 1 cut(s) 771
BshFI GGCC 2 cut(s) 111, 125
BshNI GGYRCC 2 cut(s) 159, 431
BsiHKAI GWGCWC 2 cut(s) 78, 592
BslI CCNNNNNNNGG 2 cut(s) 121, 584
BsmAI GTCTC 3 cut(s) 98, 176, 537
BsnI GGCC 2 cut(s) 111, 125
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 59, 78, 592
Bsp143I GATC 3 cut(s) 391, 573, 714
BspACI CCGC 2 cut(s) 612, 724
BspANI GGCC 2 cut(s) 111, 125
BspCNI CTCAG 2 cut(s) 160, 556
BspLI GGNNCC 5 cut(s) 161, 228, 433, 575, 628
BspPI GGATC 2 cut(s) 568, 581
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 2 cut(s) 159, 431
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 655
BsrI ACTGG 5 cut(s) 57, 94, 112, 126, 339
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 3 cut(s) 391, 573, 714
Bst2UI CCWGG 3 cut(s) 420, 436, 585
Bst4CI ACNGT 2 cut(s) 159, 364
Bst6I CTCTTC 3 cut(s) 375, 705, 776
BstAPI GCANNNNNTGC 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 3 cut(s) 147, 414, 564
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 394, 499, 767
BstKTI GATC 3 cut(s) 394, 576, 717
BstMAI GTCTC 3 cut(s) 98, 176, 537
BstMBI GATC 3 cut(s) 391, 573, 714
BstMWI GCNNNNNNNGC 2 cut(s) 96, 159
BstNI CCWGG 3 cut(s) 420, 436, 585
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 3 cut(s) 418, 434, 583
BstV1I GCAGC 1 cut(s) 771
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 2 cut(s) 573, 714
BstYI RGATCY 2 cut(s) 573, 714
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 2 cut(s) 111, 125
BtsCI GGATG 3 cut(s) 394, 499, 767
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 2 cut(s) 101, 795
Cac8I GCNNGC 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 226, 752
CseI GACGC 1 cut(s) 88
CviAII CATG 2 cut(s) 214, 801
DdeI CTNAG 3 cut(s) 147, 414, 564
DpnI GATC 3 cut(s) 393, 575, 716
DpnII GATC 3 cut(s) 391, 573, 714
EaeI YGGCCR 2 cut(s) 109, 123
Eam1104I CTCTTC 3 cut(s) 375, 705, 776
EarI CTCTTC 3 cut(s) 375, 705, 776
Ecl136II GAGCTC 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 59, 78
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 226, 752
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 576
EcoICRI GAGCTC 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 418, 434, 583
EcoT38I GRGCYC 2 cut(s) 59, 78
FaeI CATG 2 cut(s) 217, 804
FaiI YATR 5 cut(s) 195, 215, 306, 743, 802
FatI CATG 2 cut(s) 213, 800
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 1 cut(s) 760
FokI GGATG 3 cut(s) 401, 506, 754
FriOI GRGCYC 2 cut(s) 59, 78
Fsp4HI GCNGC 1 cut(s) 760
FspBI CTAG 1 cut(s) 537
GluI GCNGC 1 cut(s) 760
HaeIII GGCC 2 cut(s) 111, 125
HgaI GACGC 1 cut(s) 88
Hin1II CATG 2 cut(s) 217, 804
HinfI GANTC 6 cut(s) 137, 321, 551, 632, 638, 704
HphI GGTGA 2 cut(s) 512, 626
Hpy188I TCNGA 8 cut(s) 150, 286, 520, 556, 631, 637, 643, 709
Hpy188III TCNNGA 2 cut(s) 103, 771
Hpy99I CGWCG 2 cut(s) 10, 104
HpyAV CCTTC 4 cut(s) 32, 76, 433, 466
HpyCH4III ACNGT 2 cut(s) 159, 364
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 2 cut(s) 767, 800
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 159
HpyF3I CTNAG 3 cut(s) 147, 414, 564
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 2 cut(s) 217, 804
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 391, 573, 714
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 2 cut(s) 73, 587
Lsp1109I GCAGC 1 cut(s) 771
LweI GCATC 1 cut(s) 776
MaeI CTAG 1 cut(s) 537
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 1 cut(s) 614
MalI GATC 3 cut(s) 393, 575, 716
MboI GATC 3 cut(s) 391, 573, 714
MflI RGATCY 2 cut(s) 573, 714
MhlI GDGCHC 3 cut(s) 59, 78, 592
MlsI TGGCCA 2 cut(s) 111, 125
MluCI AATT 4 cut(s) 116, 179, 681, 747
MluNI TGGCCA 2 cut(s) 111, 125
MlyI GAGTC 2 cut(s) 647, 713
Mox20I TGGCCA 2 cut(s) 111, 125
MroXI GAANNNNTTC 1 cut(s) 320
MscI TGGCCA 2 cut(s) 111, 125
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 2 cut(s) 111, 125
MspR9I CCNGG 3 cut(s) 420, 436, 585
MvaI CCWGG 3 cut(s) 420, 436, 585
MwoI GCNNNNNNNGC 2 cut(s) 96, 159
NdeII GATC 3 cut(s) 391, 573, 714
NlaIII CATG 2 cut(s) 217, 804
NlaIV GGNNCC 5 cut(s) 161, 228, 433, 575, 628
NmuCI GTSAC 1 cut(s) 614
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 4 cut(s) 137, 321, 551, 632
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 1 cut(s) 761
PleI GAGTC 2 cut(s) 646, 712
PpsI GAGTC 2 cut(s) 646, 712
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 213
PshBI ATTAAT 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 3 cut(s) 418, 434, 583
PspGI CCWGG 3 cut(s) 418, 434, 583
PspN4I GGNNCC 5 cut(s) 161, 228, 433, 575, 628
PspPI GGNCC 2 cut(s) 226, 752
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 2 cut(s) 573, 714
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 760
Sau3AI GATC 3 cut(s) 391, 573, 714
Sau96I GGNCC 2 cut(s) 226, 752
SchI GAGTC 2 cut(s) 647, 713
ScrFI CCNGG 3 cut(s) 420, 436, 585
SduI GDGCHC 3 cut(s) 59, 78, 592
SfaNI GCATC 1 cut(s) 776
SinI GGWCC 2 cut(s) 226, 752
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 4 cut(s) 116, 179, 681, 747
SsiI CCGC 2 cut(s) 612, 724
SspMI CTAG 1 cut(s) 537
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 418, 434, 583
TaaI ACNGT 2 cut(s) 159, 364
TaiI ACGT 1 cut(s) 595
TaqI TCGA 3 cut(s) 78, 102, 177
TasI AATT 4 cut(s) 116, 179, 681, 747
TfiI GAWTC 4 cut(s) 137, 321, 551, 632
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 101, 802
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 1 cut(s) 759
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 101, 802
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 226, 752
VspI ATTAAT 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.