Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1009 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 583 |
| AatII | GACGTC | 1 cut(s) | 581 |
| Acc36I | ACCTGC | 1 cut(s) | 1009 |
| AccB1I | GGYRCC | 2 cut(s) | 754, 1421 |
| AccI | GTMKAC | 1 cut(s) | 972 |
| AciI | CCGC | 5 cut(s) | 187, 248, 305, 1001, 1349 |
| AclWI | GGATC | 5 cut(s) | 764, 876, 1055, 1068, 1085 |
| AcsI | RAATTY | 2 cut(s) | 175, 737 |
| AcuI | CTGAAG | 1 cut(s) | 1292 |
| AcyI | GRCGYC | 1 cut(s) | 578 |
| AfaI | GTAC | 2 cut(s) | 148, 1232 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 374, 725, 1100 |
| AgsI | TTSAA | 5 cut(s) | 470, 943, 1120, 1211, 1360 |
| AjiI | CACGTC | 2 cut(s) | 585, 686 |
| AjnI | CCWGG | 1 cut(s) | 864 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1269, 1301 |
| AluBI | AGCT | 2 cut(s) | 1111, 1367 |
| AluI | AGCT | 2 cut(s) | 1111, 1367 |
| Alw21I | GWGCWC | 1 cut(s) | 1485 |
| Alw26I | GTCTC | 1 cut(s) | 1357 |
| AlwI | GGATC | 5 cut(s) | 764, 876, 1055, 1068, 1085 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 329 |
| ApeKI | GCWGC | 2 cut(s) | 107, 110 |
| ApoI | RAATTY | 2 cut(s) | 175, 737 |
| AseI | ATTAAT | 1 cut(s) | 1476 |
| AspS9I | GGNCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| AsuHPI | GGTGA | 7 cut(s) | 171, 237, 284, 833, 844, 1258, 1286 |
| AsuII | TTCGAA | 1 cut(s) | 160 |
| AvaII | GGWCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| BamHI | GGATCC | 1 cut(s) | 1060 |
| BanI | GGYRCC | 2 cut(s) | 754, 1421 |
| BbsI | GAAGAC | 3 cut(s) | 477, 1046, 1218 |
| Bbv12I | GWGCWC | 1 cut(s) | 1485 |
| BbvI | GCAGC | 2 cut(s) | 119, 122 |
| BccI | CCATC | 5 cut(s) | 217, 263, 368, 613, 1277 |
| BceAI | ACGGC | 2 cut(s) | 352, 1206 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 302, 336 |
| BciT130I | CCWGG | 1 cut(s) | 866 |
| BciVI | GTATCC | 1 cut(s) | 610 |
| BcoDI | GTCTC | 1 cut(s) | 1357 |
| BfaI | CTAG | 3 cut(s) | 878, 887, 1492 |
| BfuAI | ACCTGC | 1 cut(s) | 1009 |
| BfuI | GTATCC | 1 cut(s) | 610 |
| BisI | GCNGC | 2 cut(s) | 108, 111 |
| BlsI | GCNGC | 2 cut(s) | 109, 112 |
| Bme1390I | CCNGG | 1 cut(s) | 866 |
| Bme18I | GGWCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| BmgBI | CACGTC | 2 cut(s) | 585, 686 |
| BmgT120I | GGNCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| BmiI | GGNNCC | 7 cut(s) | 228, 356, 438, 756, 1062, 1105, 1423 |
| BmrFI | CCNGG | 1 cut(s) | 866 |
| BmrI | ACTGGG | 1 cut(s) | 352 |
| BmuI | ACTGGG | 1 cut(s) | 352 |
| BoxI | GACNNNNGTC | 1 cut(s) | 694 |
| BpiI | GAAGAC | 3 cut(s) | 477, 1046, 1218 |
| Bpu14I | TTCGAA | 1 cut(s) | 160 |
| BpuEI | CTTGAG | 2 cut(s) | 1014, 1166 |
| BsaBI | GATNNNNATC | 2 cut(s) | 768, 873 |
| BsaHI | GRCGYC | 1 cut(s) | 578 |
| BsaI | GGTCTC | 1 cut(s) | 1357 |
| BsaWI | WCCGGW | 2 cut(s) | 88, 1063 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 885, 915 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 374, 725, 1100 |
| Bse1I | ACTGG | 1 cut(s) | 358 |
| Bse3DI | GCAATG | 1 cut(s) | 745 |
| Bse8I | GATNNNNATC | 2 cut(s) | 768, 873 |
| BseBI | CCWGG | 1 cut(s) | 866 |
| BseGI | GGATG | 3 cut(s) | 85, 271, 1429 |
| BseJI | GATNNNNATC | 2 cut(s) | 768, 873 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 374, 725, 1100 |
| BseMI | GCAATG | 1 cut(s) | 745 |
| BseMII | CTCAG | 2 cut(s) | 348, 990 |
| BseNI | ACTGG | 1 cut(s) | 358 |
| BseXI | GCAGC | 2 cut(s) | 119, 122 |
| BseYI | CCCAGC | 1 cut(s) | 1107 |
| BshNI | GGYRCC | 2 cut(s) | 754, 1421 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1485 |
| BsiSI | CCGG | 2 cut(s) | 89, 1064 |
| BslFI | GGGAC | 1 cut(s) | 154 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 374, 725, 1100 |
| BsmAI | GTCTC | 1 cut(s) | 1357 |
| BsmFI | GGGAC | 1 cut(s) | 154 |
| Bso31I | GGTCTC | 1 cut(s) | 1357 |
| Bsp119I | TTCGAA | 1 cut(s) | 160 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1485 |
| Bsp143I | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| BspACI | CCGC | 5 cut(s) | 187, 248, 305, 1001, 1349 |
| BspCNI | CTCAG | 2 cut(s) | 347, 989 |
| BspLI | GGNNCC | 7 cut(s) | 228, 356, 438, 756, 1062, 1105, 1423 |
| BspMI | ACCTGC | 1 cut(s) | 1009 |
| BspPI | GGATC | 5 cut(s) | 764, 876, 1055, 1068, 1085 |
| BspT104I | TTCGAA | 1 cut(s) | 160 |
| BspT107I | GGYRCC | 2 cut(s) | 754, 1421 |
| BspTNI | GGTCTC | 1 cut(s) | 1357 |
| BsrDI | GCAATG | 1 cut(s) | 745 |
| BsrI | ACTGG | 1 cut(s) | 358 |
| BssMI | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| BssNI | GRCGYC | 1 cut(s) | 578 |
| Bst2UI | CCWGG | 1 cut(s) | 866 |
| Bst4CI | ACNGT | 4 cut(s) | 641, 827, 901, 1045 |
| Bst6I | CTCTTC | 1 cut(s) | 1441 |
| BstACI | GRCGYC | 1 cut(s) | 578 |
| BstBI | TTCGAA | 1 cut(s) | 160 |
| BstC8I | GCNNGC | 4 cut(s) | 365, 396, 784, 1365 |
| BstDEI | CTNAG | 2 cut(s) | 334, 976 |
| BstF5I | GGATG | 3 cut(s) | 85, 271, 1429 |
| BstKTI | GATC | 7 cut(s) | 772, 792, 871, 1063, 1093, 1326, 1336 |
| BstMAI | GTCTC | 1 cut(s) | 1357 |
| BstMBI | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| BstNI | CCWGG | 1 cut(s) | 866 |
| BstNSI | RCATGY | 1 cut(s) | 398 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 694 |
| BstSCI | CCNGG | 1 cut(s) | 864 |
| BstV1I | GCAGC | 2 cut(s) | 119, 122 |
| BstV2I | GAAGAC | 3 cut(s) | 477, 1046, 1218 |
| BstX2I | RGATCY | 1 cut(s) | 1060 |
| BstYI | RGATCY | 1 cut(s) | 1060 |
| BsuI | GTATCC | 1 cut(s) | 610 |
| BtrI | CACGTC | 2 cut(s) | 585, 686 |
| BtsCI | GGATG | 3 cut(s) | 85, 271, 1429 |
| BtsIMutI | CAGTG | 2 cut(s) | 365, 646 |
| BveI | ACCTGC | 1 cut(s) | 1009 |
| Cac8I | GCNNGC | 4 cut(s) | 365, 396, 784, 1365 |
| CaiI | CAGNNNCTG | 1 cut(s) | 329 |
| Cfr13I | GGNCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| CseI | GACGC | 1 cut(s) | 892 |
| Csp6I | GTAC | 2 cut(s) | 147, 1231 |
| CviAII | CATG | 4 cut(s) | 395, 400, 964, 1156 |
| CviJI | RGCY | 8 cut(s) | 27, 367, 557, 595, 679, 1111, 1363, 1367 |
| CviKI_1 | RGCY | 8 cut(s) | 27, 367, 557, 595, 679, 1111, 1363, 1367 |
| CviQI | GTAC | 2 cut(s) | 147, 1231 |
| DdeI | CTNAG | 2 cut(s) | 334, 976 |
| DpnI | GATC | 7 cut(s) | 771, 791, 870, 1062, 1092, 1325, 1335 |
| DpnII | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 583 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 583 |
| Eam1104I | CTCTTC | 1 cut(s) | 1441 |
| EarI | CTCTTC | 1 cut(s) | 1441 |
| EciI | GGCGGA | 1 cut(s) | 294 |
| Eco31I | GGTCTC | 1 cut(s) | 1357 |
| Eco47I | GGWCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| Eco57I | CTGAAG | 1 cut(s) | 1292 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1142 |
| EcoRII | CCWGG | 1 cut(s) | 864 |
| EcoT22I | ATGCAT | 1 cut(s) | 1161 |
| FaeI | CATG | 4 cut(s) | 398, 403, 967, 1159 |
| FaqI | GGGAC | 1 cut(s) | 154 |
| FatI | CATG | 4 cut(s) | 394, 399, 963, 1155 |
| FblI | GTMKAC | 1 cut(s) | 972 |
| Fnu4HI | GCNGC | 2 cut(s) | 108, 111 |
| FokI | GGATG | 3 cut(s) | 72, 278, 1416 |
| Fsp4HI | GCNGC | 2 cut(s) | 108, 111 |
| FspBI | CTAG | 3 cut(s) | 878, 887, 1492 |
| GluI | GCNGC | 2 cut(s) | 108, 111 |
| GsaI | CCCAGC | 1 cut(s) | 1111 |
| HapII | CCGG | 2 cut(s) | 89, 1064 |
| HgaI | GACGC | 1 cut(s) | 892 |
| Hin1I | GRCGYC | 1 cut(s) | 578 |
| Hin1II | CATG | 4 cut(s) | 398, 403, 967, 1159 |
| HincII | GTYRAC | 2 cut(s) | 973, 1188 |
| HindII | GTYRAC | 2 cut(s) | 973, 1188 |
| HinfI | GANTC | 5 cut(s) | 332, 480, 533, 974, 1132 |
| HpaI | GTTAAC | 1 cut(s) | 1188 |
| HpaII | CCGG | 2 cut(s) | 89, 1064 |
| HphI | GGTGA | 7 cut(s) | 171, 237, 284, 833, 844, 1258, 1286 |
| Hpy166II | GTNNAC | 6 cut(s) | 275, 582, 689, 973, 1188, 1249 |
| Hpy188I | TCNGA | 5 cut(s) | 905, 1053, 1095, 1137, 1264 |
| Hpy188III | TCNNGA | 6 cut(s) | 467, 574, 616, 978, 1321, 1337 |
| Hpy8I | GTNNAC | 6 cut(s) | 275, 582, 689, 973, 1188, 1249 |
| Hpy99I | CGWCG | 3 cut(s) | 219, 314, 905 |
| HpyAV | CCTTC | 6 cut(s) | 38, 193, 427, 884, 1338, 1366 |
| HpyCH4III | ACNGT | 4 cut(s) | 641, 827, 901, 1045 |
| HpyCH4IV | ACGT | 5 cut(s) | 217, 578, 584, 685, 839 |
| HpyCH4V | TGCA | 7 cut(s) | 107, 323, 363, 507, 782, 1159, 1374 |
| HpyF3I | CTNAG | 2 cut(s) | 334, 976 |
| HpySE526I | ACGT | 5 cut(s) | 217, 578, 584, 685, 839 |
| Hsp92I | GRCGYC | 1 cut(s) | 578 |
| Hsp92II | CATG | 4 cut(s) | 398, 403, 967, 1159 |
| KspAI | GTTAAC | 1 cut(s) | 1188 |
| Kzo9I | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| Lsp1109I | GCAGC | 2 cut(s) | 119, 122 |
| MaeI | CTAG | 3 cut(s) | 878, 887, 1492 |
| MaeII | ACGT | 5 cut(s) | 217, 578, 584, 685, 839 |
| MaeIII | GTNAC | 8 cut(s) | 821, 832, 880, 895, 959, 1024, 1150, 1391 |
| MalI | GATC | 7 cut(s) | 771, 791, 870, 1062, 1092, 1325, 1335 |
| MboI | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| MboII | GAAGA | 7 cut(s) | 482, 1048, 1051, 1141, 1223, 1298, 1428 |
| MflI | RGATCY | 1 cut(s) | 1060 |
| MhlI | GDGCHC | 1 cut(s) | 1485 |
| MluCI | AATT | 5 cut(s) | 175, 567, 711, 737, 1055 |
| MlyI | GAGTC | 3 cut(s) | 326, 527, 968 |
| MmeI | TCCRAC | 3 cut(s) | 706, 883, 1118 |
| MnlI | CCTC | 7 cut(s) | 256, 722, 729, 1093, 1159, 1264, 1444 |
| Mph1103I | ATGCAT | 1 cut(s) | 1161 |
| MseI | TTAA | 6 cut(s) | 9, 120, 650, 1047, 1187, 1476 |
| MslI | CAYNNNNRTG | 2 cut(s) | 77, 1379 |
| MspA1I | CMGCKG | 1 cut(s) | 1367 |
| MspI | CCGG | 2 cut(s) | 89, 1064 |
| MspR9I | CCNGG | 1 cut(s) | 866 |
| MvaI | CCWGG | 1 cut(s) | 866 |
| NdeII | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| NlaIII | CATG | 4 cut(s) | 398, 403, 967, 1159 |
| NlaIV | GGNNCC | 7 cut(s) | 228, 356, 438, 756, 1062, 1105, 1423 |
| NmuCI | GTSAC | 3 cut(s) | 821, 832, 880 |
| NsiI | ATGCAT | 1 cut(s) | 1161 |
| NspI | RCATGY | 1 cut(s) | 398 |
| NspV | TTCGAA | 1 cut(s) | 160 |
| PaeI | GCATGC | 1 cut(s) | 398 |
| PaqCI | CACCTGC | 1 cut(s) | 1009 |
| PfeI | GAWTC | 2 cut(s) | 480, 1132 |
| PflFI | GACNNNGTC | 1 cut(s) | 838 |
| PfoI | TCCNGGA | 1 cut(s) | 864 |
| PkrI | GCNGC | 2 cut(s) | 109, 112 |
| PleI | GAGTC | 3 cut(s) | 326, 527, 968 |
| PpsI | GAGTC | 3 cut(s) | 326, 527, 968 |
| PpuMI | RGGWCCY | 1 cut(s) | 1142 |
| PshAI | GACNNNNGTC | 1 cut(s) | 694 |
| PshBI | ATTAAT | 1 cut(s) | 1476 |
| Psp5II | RGGWCCY | 1 cut(s) | 1142 |
| Psp6I | CCWGG | 1 cut(s) | 864 |
| PspFI | CCCAGC | 1 cut(s) | 1107 |
| PspGI | CCWGG | 1 cut(s) | 864 |
| PspN4I | GGNNCC | 7 cut(s) | 228, 356, 438, 756, 1062, 1105, 1423 |
| PspPI | GGNCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| PspPPI | RGGWCCY | 1 cut(s) | 1142 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1214, 1246 |
| PstNI | CAGNNNCTG | 1 cut(s) | 329 |
| PsuI | RGATCY | 1 cut(s) | 1060 |
| PsyI | GACNNNGTC | 1 cut(s) | 838 |
| PvuII | CAGCTG | 1 cut(s) | 1367 |
| RsaI | GTAC | 2 cut(s) | 148, 1232 |
| RsaNI | GTAC | 2 cut(s) | 147, 1231 |
| RseI | CAYNNNNRTG | 2 cut(s) | 77, 1379 |
| SalI | GTCGAC | 1 cut(s) | 971 |
| SaqAI | TTAA | 6 cut(s) | 9, 120, 650, 1047, 1187, 1476 |
| SatI | GCNGC | 2 cut(s) | 108, 111 |
| Sau3AI | GATC | 7 cut(s) | 769, 789, 868, 1060, 1090, 1323, 1333 |
| Sau96I | GGNCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| SchI | GAGTC | 3 cut(s) | 326, 527, 968 |
| ScrFI | CCNGG | 1 cut(s) | 866 |
| SduI | GDGCHC | 1 cut(s) | 1485 |
| SfuI | TTCGAA | 1 cut(s) | 160 |
| SinI | GGWCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 77, 1379 |
| SmlI | CTYRAG | 2 cut(s) | 1029, 1181 |
| SmoI | CTYRAG | 2 cut(s) | 1029, 1181 |
| SphI | GCATGC | 1 cut(s) | 398 |
| Sse9I | AATT | 5 cut(s) | 175, 567, 711, 737, 1055 |
| SsiI | CCGC | 5 cut(s) | 187, 248, 305, 1001, 1349 |
| SspI | AATATT | 1 cut(s) | 118 |
| SspMI | CTAG | 3 cut(s) | 878, 887, 1492 |
| StyD4I | CCNGG | 1 cut(s) | 864 |
| TaaI | ACNGT | 4 cut(s) | 641, 827, 901, 1045 |
| TaiI | ACGT | 5 cut(s) | 220, 581, 587, 688, 842 |
| TaqI | TCGA | 5 cut(s) | 160, 214, 312, 405, 972 |
| TasI | AATT | 5 cut(s) | 175, 567, 711, 737, 1055 |
| TatI | WGTACW | 1 cut(s) | 1230 |
| TfiI | GAWTC | 2 cut(s) | 480, 1132 |
| Tru1I | TTAA | 6 cut(s) | 9, 120, 650, 1047, 1187, 1476 |
| Tru9I | TTAA | 6 cut(s) | 9, 120, 650, 1047, 1187, 1476 |
| TscAI | CASTG | 2 cut(s) | 365, 646 |
| TseFI | GTSAC | 3 cut(s) | 821, 832, 880 |
| TseI | GCWGC | 2 cut(s) | 107, 110 |
| Tsp45I | GTSAC | 3 cut(s) | 821, 832, 880 |
| TspDTI | ATGAA | 6 cut(s) | 20, 74, 299, 404, 1088, 1428 |
| TspGWI | ACGGA | 1 cut(s) | 909 |
| TspRI | CASTG | 2 cut(s) | 365, 646 |
| Tth111I | GACNNNGTC | 1 cut(s) | 838 |
| VpaK11BI | GGWCC | 5 cut(s) | 227, 354, 689, 716, 1142 |
| VspI | ATTAAT | 1 cut(s) | 1476 |
| XapI | RAATTY | 2 cut(s) | 175, 737 |
| XceI | RCATGY | 1 cut(s) | 398 |
| XmiI | GTMKAC | 1 cut(s) | 972 |
| XspI | CTAG | 3 cut(s) | 878, 887, 1492 |
| ZraI | GACGTC | 1 cut(s) | 579 |
| Zsp2I | ATGCAT | 1 cut(s) | 1161 |