Belongs to the peptidase A1 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Vac_ImportDeg | PF09783 | 94 - 180 | 1.2e-22 | Vacuolar import and degradation protein |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 543, 661 |
| AccIII | TCCGGA | 1 cut(s) | 889 |
| AciI | CCGC | 1 cut(s) | 254 |
| AclWI | GGATC | 1 cut(s) | 536 |
| AcoI | YGGCCR | 1 cut(s) | 277 |
| AcsI | RAATTY | 2 cut(s) | 386, 639 |
| AfaI | GTAC | 2 cut(s) | 618, 743 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 877 |
| AflII | CTTAAG | 1 cut(s) | 875 |
| AgsI | TTSAA | 6 cut(s) | 57, 238, 245, 653, 713, 969 |
| AjnI | CCWGG | 3 cut(s) | 279, 797, 883 |
| AluBI | AGCT | 3 cut(s) | 171, 691, 721 |
| AluI | AGCT | 3 cut(s) | 171, 691, 721 |
| Alw26I | GTCTC | 2 cut(s) | 89, 633 |
| AlwI | GGATC | 1 cut(s) | 536 |
| Aor13HI | TCCGGA | 1 cut(s) | 889 |
| AoxI | GGCC | 3 cut(s) | 277, 563, 868 |
| ApeKI | GCWGC | 1 cut(s) | 171 |
| ApoI | RAATTY | 2 cut(s) | 386, 639 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 640, 643 |
| AspS9I | GGNCC | 3 cut(s) | 381, 869, 887 |
| AsuHPI | GGTGA | 1 cut(s) | 280 |
| AvaII | GGWCC | 2 cut(s) | 381, 887 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 473, 505 |
| BbsI | GAAGAC | 1 cut(s) | 811 |
| BbvI | GCAGC | 1 cut(s) | 158 |
| BccI | CCATC | 5 cut(s) | 176, 422, 471, 530, 867 |
| BceAI | ACGGC | 1 cut(s) | 544 |
| BciT130I | CCWGG | 3 cut(s) | 281, 799, 885 |
| BcoDI | GTCTC | 2 cut(s) | 89, 633 |
| BfaI | CTAG | 1 cut(s) | 836 |
| BfmI | CTRYAG | 1 cut(s) | 767 |
| BfrI | CTTAAG | 1 cut(s) | 875 |
| BfuAI | ACCTGC | 2 cut(s) | 543, 661 |
| BglII | AGATCT | 1 cut(s) | 445 |
| BisI | GCNGC | 1 cut(s) | 172 |
| BlsI | GCNGC | 1 cut(s) | 173 |
| Bme1390I | CCNGG | 3 cut(s) | 281, 799, 885 |
| Bme18I | GGWCC | 2 cut(s) | 381, 887 |
| BmgT120I | GGNCC | 3 cut(s) | 381, 869, 887 |
| BmiI | GGNNCC | 5 cut(s) | 147, 520, 803, 882, 929 |
| BmrFI | CCNGG | 3 cut(s) | 281, 799, 885 |
| BmsI | GCATC | 1 cut(s) | 236 |
| BpiI | GAAGAC | 1 cut(s) | 811 |
| BpmI | CTGGAG | 1 cut(s) | 867 |
| BsaI | GGTCTC | 1 cut(s) | 89 |
| BsaJI | CCNNGG | 1 cut(s) | 139 |
| BsaWI | WCCGGW | 1 cut(s) | 889 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 503, 533 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 877 |
| Bse1I | ACTGG | 1 cut(s) | 383 |
| BseAI | TCCGGA | 1 cut(s) | 889 |
| BseBI | CCWGG | 3 cut(s) | 281, 799, 885 |
| BseDI | CCNNGG | 1 cut(s) | 139 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 877 |
| BseMII | CTCAG | 2 cut(s) | 330, 463 |
| BseNI | ACTGG | 1 cut(s) | 383 |
| BseXI | GCAGC | 1 cut(s) | 158 |
| BseYI | CCCAGC | 1 cut(s) | 691 |
| BshFI | GGCC | 3 cut(s) | 279, 565, 870 |
| BsiSI | CCGG | 1 cut(s) | 890 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 877 |
| BsmAI | GTCTC | 2 cut(s) | 89, 633 |
| BsmI | GAATGC | 1 cut(s) | 759 |
| BsnI | GGCC | 3 cut(s) | 279, 565, 870 |
| Bso31I | GGTCTC | 1 cut(s) | 89 |
| Bsp13I | TCCGGA | 1 cut(s) | 889 |
| Bsp143I | GATC | 2 cut(s) | 445, 541 |
| BspACI | CCGC | 1 cut(s) | 254 |
| BspANI | GGCC | 3 cut(s) | 279, 565, 870 |
| BspCNI | CTCAG | 2 cut(s) | 331, 462 |
| BspEI | TCCGGA | 1 cut(s) | 889 |
| BspHI | TCATGA | 1 cut(s) | 471 |
| BspLI | GGNNCC | 5 cut(s) | 147, 520, 803, 882, 929 |
| BspMI | ACCTGC | 2 cut(s) | 543, 661 |
| BspPI | GGATC | 1 cut(s) | 536 |
| BspQI | GCTCTTC | 1 cut(s) | 711 |
| BspTI | CTTAAG | 1 cut(s) | 875 |
| BspTNI | GGTCTC | 1 cut(s) | 89 |
| BsrI | ACTGG | 1 cut(s) | 383 |
| BssECI | CCNNGG | 1 cut(s) | 139 |
| BssMI | GATC | 2 cut(s) | 445, 541 |
| BssT1I | CCWWGG | 1 cut(s) | 139 |
| Bst2UI | CCWGG | 3 cut(s) | 281, 799, 885 |
| Bst4CI | ACNGT | 2 cut(s) | 515, 627 |
| Bst6I | CTCTTC | 2 cut(s) | 13, 711 |
| BstAFI | CTTAAG | 1 cut(s) | 875 |
| BstC8I | GCNNGC | 1 cut(s) | 169 |
| BstDEI | CTNAG | 2 cut(s) | 339, 449 |
| BstKTI | GATC | 2 cut(s) | 448, 544 |
| BstMAI | GTCTC | 2 cut(s) | 89, 633 |
| BstMBI | GATC | 2 cut(s) | 445, 541 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 591, 763 |
| BstNI | CCWGG | 3 cut(s) | 281, 799, 885 |
| BstSCI | CCNGG | 3 cut(s) | 279, 797, 883 |
| BstSFI | CTRYAG | 1 cut(s) | 767 |
| BstV1I | GCAGC | 1 cut(s) | 158 |
| BstV2I | GAAGAC | 1 cut(s) | 811 |
| BstX2I | RGATCY | 1 cut(s) | 445 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 866 |
| BstYI | RGATCY | 1 cut(s) | 445 |
| BsuRI | GGCC | 3 cut(s) | 279, 565, 870 |
| BtsI | GCAGTG | 1 cut(s) | 985 |
| BtsIMutI | CAGTG | 3 cut(s) | 336, 376, 985 |
| BveI | ACCTGC | 2 cut(s) | 543, 661 |
| Cac8I | GCNNGC | 1 cut(s) | 169 |
| CciI | TCATGA | 1 cut(s) | 471 |
| Cfr13I | GGNCC | 3 cut(s) | 381, 869, 887 |
| CseI | GACGC | 1 cut(s) | 262 |
| Csp6I | GTAC | 2 cut(s) | 617, 742 |
| CviAII | CATG | 2 cut(s) | 472, 902 |
| CviQI | GTAC | 2 cut(s) | 617, 742 |
| DdeI | CTNAG | 2 cut(s) | 339, 449 |
| DpnI | GATC | 2 cut(s) | 447, 543 |
| DpnII | GATC | 2 cut(s) | 445, 541 |
| EaeI | YGGCCR | 1 cut(s) | 277 |
| Eam1104I | CTCTTC | 2 cut(s) | 13, 711 |
| EarI | CTCTTC | 2 cut(s) | 13, 711 |
| Eco130I | CCWWGG | 1 cut(s) | 139 |
| Eco31I | GGTCTC | 1 cut(s) | 89 |
| Eco47I | GGWCC | 2 cut(s) | 381, 887 |
| EcoRII | CCWGG | 3 cut(s) | 279, 797, 883 |
| EcoT14I | CCWWGG | 1 cut(s) | 139 |
| EcoT22I | ATGCAT | 1 cut(s) | 128 |
| ErhI | CCWWGG | 1 cut(s) | 139 |
| FaeI | CATG | 2 cut(s) | 475, 905 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 628, 660, 705, 737 |
| FatI | CATG | 2 cut(s) | 471, 901 |
| FauI | CCCGC | 1 cut(s) | 261 |
| Fnu4HI | GCNGC | 1 cut(s) | 172 |
| Fsp4HI | GCNGC | 1 cut(s) | 172 |
| FspBI | CTAG | 1 cut(s) | 836 |
| GluI | GCNGC | 1 cut(s) | 172 |
| GsaI | CCCAGC | 1 cut(s) | 695 |
| GsuI | CTGGAG | 1 cut(s) | 867 |
| HaeIII | GGCC | 3 cut(s) | 279, 565, 870 |
| HapII | CCGG | 1 cut(s) | 890 |
| HgaI | GACGC | 1 cut(s) | 262 |
| Hin1II | CATG | 2 cut(s) | 475, 905 |
| HincII | GTYRAC | 1 cut(s) | 313 |
| HindII | GTYRAC | 1 cut(s) | 313 |
| HinfI | GANTC | 2 cut(s) | 53, 897 |
| HpaII | CCGG | 1 cut(s) | 890 |
| HphI | GGTGA | 1 cut(s) | 280 |
| Hpy166II | GTNNAC | 2 cut(s) | 313, 847 |
| Hpy188I | TCNGA | 5 cut(s) | 29, 52, 133, 635, 752 |
| Hpy188III | TCNNGA | 4 cut(s) | 472, 713, 836, 890 |
| Hpy8I | GTNNAC | 2 cut(s) | 313, 847 |
| Hpy99I | CGWCG | 1 cut(s) | 278 |
| HpyAV | CCTTC | 6 cut(s) | 216, 232, 268, 293, 402, 475 |
| HpyCH4III | ACNGT | 2 cut(s) | 515, 627 |
| HpyCH4V | TGCA | 3 cut(s) | 126, 227, 854 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 591, 763 |
| HpyF3I | CTNAG | 2 cut(s) | 339, 449 |
| Hsp92II | CATG | 2 cut(s) | 475, 905 |
| Kpn2I | TCCGGA | 1 cut(s) | 889 |
| Kzo9I | GATC | 2 cut(s) | 445, 541 |
| LguI | GCTCTTC | 1 cut(s) | 711 |
| LmnI | GCTCC | 4 cut(s) | 484, 524, 807, 886 |
| Lsp1109I | GCAGC | 1 cut(s) | 158 |
| LweI | GCATC | 1 cut(s) | 236 |
| MaeI | CTAG | 1 cut(s) | 836 |
| MaeIII | GTNAC | 3 cut(s) | 286, 789, 829 |
| MalI | GATC | 2 cut(s) | 447, 543 |
| MboI | GATC | 2 cut(s) | 445, 541 |
| MflI | RGATCY | 1 cut(s) | 445 |
| MluCI | AATT | 5 cut(s) | 12, 322, 386, 639, 955 |
| MmeI | TCCRAC | 1 cut(s) | 829 |
| MnlI | CCTC | 3 cut(s) | 63, 220, 882 |
| Mph1103I | ATGCAT | 1 cut(s) | 128 |
| MroI | TCCGGA | 1 cut(s) | 889 |
| MroXI | GAANNNNTTC | 2 cut(s) | 640, 643 |
| MseI | TTAA | 3 cut(s) | 9, 269, 876 |
| MslI | CAYNNNNRTG | 2 cut(s) | 470, 653 |
| MspCI | CTTAAG | 1 cut(s) | 875 |
| MspI | CCGG | 1 cut(s) | 890 |
| MspR9I | CCNGG | 3 cut(s) | 281, 799, 885 |
| Mva1269I | GAATGC | 1 cut(s) | 759 |
| MvaI | CCWGG | 3 cut(s) | 281, 799, 885 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 591, 763 |
| NdeII | GATC | 2 cut(s) | 445, 541 |
| NlaIII | CATG | 2 cut(s) | 475, 905 |
| NlaIV | GGNNCC | 5 cut(s) | 147, 520, 803, 882, 929 |
| NmuCI | GTSAC | 1 cut(s) | 286 |
| NsiI | ATGCAT | 1 cut(s) | 128 |
| PagI | TCATGA | 1 cut(s) | 471 |
| PciSI | GCTCTTC | 1 cut(s) | 711 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 282 |
| PctI | GAATGC | 1 cut(s) | 759 |
| PdmI | GAANNNNTTC | 2 cut(s) | 640, 643 |
| PfeI | GAWTC | 2 cut(s) | 53, 897 |
| PkrI | GCNGC | 1 cut(s) | 173 |
| Psp6I | CCWGG | 3 cut(s) | 279, 797, 883 |
| PspFI | CCCAGC | 1 cut(s) | 691 |
| PspGI | CCWGG | 3 cut(s) | 279, 797, 883 |
| PspN4I | GGNNCC | 5 cut(s) | 147, 520, 803, 882, 929 |
| PspPI | GGNCC | 3 cut(s) | 381, 869, 887 |
| PsuI | RGATCY | 1 cut(s) | 445 |
| RsaI | GTAC | 2 cut(s) | 618, 743 |
| RsaNI | GTAC | 2 cut(s) | 617, 742 |
| RseI | CAYNNNNRTG | 2 cut(s) | 470, 653 |
| SapI | GCTCTTC | 1 cut(s) | 711 |
| SaqAI | TTAA | 3 cut(s) | 9, 269, 876 |
| SatI | GCNGC | 1 cut(s) | 172 |
| Sau3AI | GATC | 2 cut(s) | 445, 541 |
| Sau96I | GGNCC | 3 cut(s) | 381, 869, 887 |
| ScrFI | CCNGG | 3 cut(s) | 281, 799, 885 |
| SfaNI | GCATC | 1 cut(s) | 236 |
| SfcI | CTRYAG | 1 cut(s) | 767 |
| SinI | GGWCC | 2 cut(s) | 381, 887 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 470, 653 |
| SmlI | CTYRAG | 1 cut(s) | 875 |
| SmoI | CTYRAG | 1 cut(s) | 875 |
| Sse9I | AATT | 5 cut(s) | 12, 322, 386, 639, 955 |
| SsiI | CCGC | 1 cut(s) | 254 |
| SspMI | CTAG | 1 cut(s) | 836 |
| StyD4I | CCNGG | 3 cut(s) | 279, 797, 883 |
| StyI | CCWWGG | 1 cut(s) | 139 |
| TaaI | ACNGT | 2 cut(s) | 515, 627 |
| TasI | AATT | 5 cut(s) | 12, 322, 386, 639, 955 |
| TfiI | GAWTC | 2 cut(s) | 53, 897 |
| Tru1I | TTAA | 3 cut(s) | 9, 269, 876 |
| Tru9I | TTAA | 3 cut(s) | 9, 269, 876 |
| TscAI | CASTG | 3 cut(s) | 343, 383, 985 |
| TseFI | GTSAC | 1 cut(s) | 286 |
| TseI | GCWGC | 1 cut(s) | 171 |
| Tsp45I | GTSAC | 1 cut(s) | 286 |
| TspDTI | ATGAA | 2 cut(s) | 460, 596 |
| TspGWI | ACGGA | 1 cut(s) | 909 |
| TspRI | CASTG | 3 cut(s) | 343, 383, 985 |
| Vha464I | CTTAAG | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 2 cut(s) | 381, 887 |
| XapI | RAATTY | 2 cut(s) | 386, 639 |
| XbaI | TCTAGA | 1 cut(s) | 835 |
| XmnI | GAANNNNTTC | 2 cut(s) | 640, 643 |
| XspI | CTAG | 1 cut(s) | 836 |
| Zsp2I | ATGCAT | 1 cut(s) | 128 |