Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 132 |
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AccI | GTMKAC | 1 cut(s) | 460 |
| AccII | CGCG | 1 cut(s) | 702 |
| AciI | CCGC | 7 cut(s) | 108, 113, 239, 305, 669, 702, 1190 |
| AclWI | GGATC | 3 cut(s) | 254, 779, 1052 |
| AcoI | YGGCCR | 1 cut(s) | 63 |
| AcsI | RAATTY | 3 cut(s) | 175, 752, 1063 |
| AfaI | GTAC | 4 cut(s) | 25, 188, 562, 781 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 740 |
| AflIII | ACRYGT | 1 cut(s) | 972 |
| AgsI | TTSAA | 7 cut(s) | 385, 589, 1101, 1117, 1301, 1351, 1480 |
| AjiI | CACGTC | 1 cut(s) | 597 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1281, 1313 |
| AluBI | AGCT | 2 cut(s) | 512, 1042 |
| AluI | AGCT | 2 cut(s) | 512, 1042 |
| Alw21I | GWGCWC | 1 cut(s) | 151 |
| Alw26I | GTCTC | 2 cut(s) | 1200, 1348 |
| AlwI | GGATC | 3 cut(s) | 254, 779, 1052 |
| AoxI | GGCC | 2 cut(s) | 63, 704 |
| ApaI | GGGCCC | 1 cut(s) | 708 |
| ApeKI | GCWGC | 5 cut(s) | 110, 339, 512, 1376, 1379 |
| ApoI | RAATTY | 3 cut(s) | 175, 752, 1063 |
| AseI | ATTAAT | 1 cut(s) | 807 |
| AspS9I | GGNCC | 4 cut(s) | 227, 704, 705, 1242 |
| AsuHPI | GGTGA | 3 cut(s) | 471, 848, 1045 |
| AvaII | GGWCC | 2 cut(s) | 227, 1242 |
| BaeGI | GKGCMC | 1 cut(s) | 708 |
| BalI | TGGCCA | 1 cut(s) | 65 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BanII | GRGCYC | 1 cut(s) | 708 |
| Bbv12I | GWGCWC | 1 cut(s) | 151 |
| BbvI | GCAGC | 5 cut(s) | 122, 351, 499, 1363, 1366 |
| BccI | CCATC | 5 cut(s) | 74, 217, 260, 1160, 1366 |
| BceAI | ACGGC | 1 cut(s) | 1203 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 474, 508 |
| BcoDI | GTCTC | 2 cut(s) | 1200, 1348 |
| BfaI | CTAG | 5 cut(s) | 251, 1248, 1266, 1383, 1472 |
| BisI | GCNGC | 7 cut(s) | 111, 114, 239, 340, 513, 1377, 1380 |
| BlsI | GCNGC | 7 cut(s) | 112, 115, 240, 341, 514, 1378, 1381 |
| BmcAI | AGTACT | 1 cut(s) | 781 |
| Bme18I | GGWCC | 2 cut(s) | 227, 1242 |
| BmgBI | CACGTC | 1 cut(s) | 597 |
| BmgT120I | GGNCC | 4 cut(s) | 227, 704, 705, 1242 |
| BmiI | GGNNCC | 3 cut(s) | 6, 228, 706 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 536, 568 |
| BpuEI | CTTGAG | 3 cut(s) | 492, 980, 996 |
| Bsa29I | ATCGAT | 1 cut(s) | 864 |
| BsaI | GGTCTC | 1 cut(s) | 1348 |
| BsaJI | CCNNGG | 1 cut(s) | 1231 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 740 |
| Bse3DI | GCAATG | 1 cut(s) | 953 |
| BseCI | ATCGAT | 1 cut(s) | 864 |
| BseDI | CCNNGG | 1 cut(s) | 1231 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 740 |
| BseMI | GCAATG | 1 cut(s) | 953 |
| BseMII | CTCAG | 1 cut(s) | 999 |
| BseSI | GKGCMC | 1 cut(s) | 708 |
| BseXI | GCAGC | 5 cut(s) | 122, 351, 499, 1363, 1366 |
| Bsh1236I | CGCG | 1 cut(s) | 702 |
| BshFI | GGCC | 2 cut(s) | 65, 706 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BshVI | ATCGAT | 1 cut(s) | 864 |
| BsiHKAI | GWGCWC | 1 cut(s) | 151 |
| BslFI | GGGAC | 1 cut(s) | 717 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 740 |
| BsmAI | GTCTC | 2 cut(s) | 1200, 1348 |
| BsmBI | CGTCTC | 1 cut(s) | 1200 |
| BsmFI | GGGAC | 1 cut(s) | 717 |
| BsnI | GGCC | 2 cut(s) | 65, 706 |
| Bso31I | GGTCTC | 1 cut(s) | 1348 |
| Bsp120I | GGGCCC | 1 cut(s) | 704 |
| Bsp1286I | GDGCHC | 2 cut(s) | 151, 708 |
| Bsp1407I | TGTACA | 1 cut(s) | 186 |
| Bsp143I | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| BspACI | CCGC | 7 cut(s) | 108, 113, 239, 305, 669, 702, 1190 |
| BspANI | GGCC | 2 cut(s) | 65, 706 |
| BspCNI | CTCAG | 1 cut(s) | 998 |
| BspDI | ATCGAT | 1 cut(s) | 864 |
| BspFNI | CGCG | 1 cut(s) | 702 |
| BspLI | GGNNCC | 3 cut(s) | 6, 228, 706 |
| BspPI | GGATC | 3 cut(s) | 254, 779, 1052 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BspTNI | GGTCTC | 1 cut(s) | 1348 |
| BsrDI | GCAATG | 1 cut(s) | 953 |
| BsrGI | TGTACA | 1 cut(s) | 186 |
| BssECI | CCNNGG | 1 cut(s) | 1231 |
| BssMI | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| BssT1I | CCWWGG | 1 cut(s) | 1231 |
| BstAUI | TGTACA | 1 cut(s) | 186 |
| BstC8I | GCNNGC | 4 cut(s) | 365, 517, 667, 704 |
| BstDEI | CTNAG | 3 cut(s) | 776, 985, 1238 |
| BstFNI | CGCG | 1 cut(s) | 702 |
| BstKTI | GATC | 5 cut(s) | 262, 787, 864, 981, 1060 |
| BstMAI | GTCTC | 2 cut(s) | 1200, 1348 |
| BstMBI | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1388, 1397 |
| BstNSI | RCATGY | 2 cut(s) | 398, 523 |
| BstSLI | GKGCMC | 1 cut(s) | 708 |
| BstUI | CGCG | 1 cut(s) | 702 |
| BstV1I | GCAGC | 5 cut(s) | 122, 351, 499, 1363, 1366 |
| Bsu15I | ATCGAT | 1 cut(s) | 864 |
| BsuRI | GGCC | 2 cut(s) | 65, 706 |
| BsuTUI | ATCGAT | 1 cut(s) | 864 |
| BtrI | CACGTC | 1 cut(s) | 597 |
| Cac8I | GCNNGC | 4 cut(s) | 365, 517, 667, 704 |
| Cfr13I | GGNCC | 4 cut(s) | 227, 704, 705, 1242 |
| ClaI | ATCGAT | 1 cut(s) | 864 |
| CseI | GACGC | 1 cut(s) | 708 |
| Csp6I | GTAC | 4 cut(s) | 24, 187, 561, 780 |
| CviAII | CATG | 4 cut(s) | 369, 395, 520, 880 |
| CviQI | GTAC | 4 cut(s) | 24, 187, 561, 780 |
| DdeI | CTNAG | 3 cut(s) | 776, 985, 1238 |
| DpnI | GATC | 5 cut(s) | 261, 786, 863, 980, 1059 |
| DpnII | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| EaeI | YGGCCR | 1 cut(s) | 63 |
| EciI | GGCGGA | 2 cut(s) | 294, 1205 |
| Eco130I | CCWWGG | 1 cut(s) | 1231 |
| Eco24I | GRGCYC | 1 cut(s) | 708 |
| Eco31I | GGTCTC | 1 cut(s) | 1348 |
| Eco32I | GATATC | 2 cut(s) | 121, 316 |
| Eco47I | GGWCC | 2 cut(s) | 227, 1242 |
| EcoO109I | RGGNCCY | 2 cut(s) | 705, 1242 |
| EcoRV | GATATC | 2 cut(s) | 121, 316 |
| EcoT14I | CCWWGG | 1 cut(s) | 1231 |
| EcoT38I | GRGCYC | 1 cut(s) | 708 |
| ErhI | CCWWGG | 1 cut(s) | 1231 |
| Esp3I | CGTCTC | 1 cut(s) | 1200 |
| FaeI | CATG | 4 cut(s) | 372, 398, 523, 883 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1244, 1276 |
| FaqI | GGGAC | 1 cut(s) | 717 |
| FatI | CATG | 4 cut(s) | 368, 394, 519, 879 |
| FauI | CCCGC | 2 cut(s) | 662, 695 |
| FblI | GTMKAC | 1 cut(s) | 460 |
| Fnu4HI | GCNGC | 7 cut(s) | 111, 114, 239, 340, 513, 1377, 1380 |
| FriOI | GRGCYC | 1 cut(s) | 708 |
| Fsp4HI | GCNGC | 7 cut(s) | 111, 114, 239, 340, 513, 1377, 1380 |
| FspBI | CTAG | 5 cut(s) | 251, 1248, 1266, 1383, 1472 |
| GluI | GCNGC | 7 cut(s) | 111, 114, 239, 340, 513, 1377, 1380 |
| HaeIII | GGCC | 2 cut(s) | 65, 706 |
| HgaI | GACGC | 1 cut(s) | 708 |
| Hin1II | CATG | 4 cut(s) | 372, 398, 523, 883 |
| HincII | GTYRAC | 2 cut(s) | 469, 852 |
| HindII | GTYRAC | 2 cut(s) | 469, 852 |
| HinfI | GANTC | 7 cut(s) | 355, 407, 416, 545, 872, 983, 1036 |
| HphI | GGTGA | 3 cut(s) | 471, 848, 1045 |
| Hpy166II | GTNNAC | 6 cut(s) | 275, 461, 469, 594, 852, 1048 |
| Hpy188I | TCNGA | 8 cut(s) | 161, 215, 448, 877, 1062, 1289, 1491, 1518 |
| Hpy188III | TCNNGA | 8 cut(s) | 230, 263, 385, 814, 1013, 1301, 1312, 1472 |
| Hpy8I | GTNNAC | 6 cut(s) | 275, 461, 469, 594, 852, 1048 |
| Hpy99I | CGWCG | 1 cut(s) | 219 |
| HpyAV | CCTTC | 2 cut(s) | 833, 1357 |
| HpyCH4IV | ACGT | 4 cut(s) | 217, 533, 596, 974 |
| HpyCH4V | TGCA | 5 cut(s) | 495, 519, 539, 1156, 1365 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1388, 1397 |
| HpyF3I | CTNAG | 3 cut(s) | 776, 985, 1238 |
| HpySE526I | ACGT | 4 cut(s) | 217, 533, 596, 974 |
| Hsp92II | CATG | 4 cut(s) | 372, 398, 523, 883 |
| Kzo9I | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| LmnI | GCTCC | 1 cut(s) | 1518 |
| LpnPI | CCDG | 9 cut(s) | 243, 276, 321, 377, 585, 933, 972, 1028, 1241 |
| Lsp1109I | GCAGC | 5 cut(s) | 122, 351, 499, 1363, 1366 |
| MaeI | CTAG | 5 cut(s) | 251, 1248, 1266, 1383, 1472 |
| MaeII | ACGT | 4 cut(s) | 217, 533, 596, 974 |
| MaeIII | GTNAC | 4 cut(s) | 610, 895, 968, 1268 |
| MalI | GATC | 5 cut(s) | 261, 786, 863, 980, 1059 |
| MboI | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| MboII | GAAGA | 3 cut(s) | 174, 1103, 1223 |
| MfeI | CAATTG | 1 cut(s) | 915 |
| MhlI | GDGCHC | 2 cut(s) | 151, 708 |
| MlsI | TGGCCA | 1 cut(s) | 65 |
| MluNI | TGGCCA | 1 cut(s) | 65 |
| MlyI | GAGTC | 4 cut(s) | 349, 401, 554, 977 |
| MmeI | TCCRAC | 2 cut(s) | 238, 721 |
| MnlI | CCTC | 9 cut(s) | 430, 492, 516, 655, 737, 783, 1020, 1063, 1255 |
| Mox20I | TGGCCA | 1 cut(s) | 65 |
| MscI | TGGCCA | 1 cut(s) | 65 |
| MseI | TTAA | 6 cut(s) | 530, 807, 939, 1184, 1448, 1527 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1370 |
| Msp20I | TGGCCA | 1 cut(s) | 65 |
| MspA1I | CMGCKG | 2 cut(s) | 113, 1042 |
| MunI | CAATTG | 1 cut(s) | 915 |
| MvnI | CGCG | 1 cut(s) | 702 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1388, 1397 |
| NdeII | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| NlaIII | CATG | 4 cut(s) | 372, 398, 523, 883 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 228, 706 |
| NmuCI | GTSAC | 2 cut(s) | 895, 1268 |
| NspI | RCATGY | 2 cut(s) | 398, 523 |
| PfeI | GAWTC | 3 cut(s) | 416, 872, 1036 |
| PkrI | GCNGC | 7 cut(s) | 112, 115, 240, 341, 514, 1378, 1381 |
| PleI | GAGTC | 4 cut(s) | 349, 401, 553, 977 |
| PpsI | GAGTC | 4 cut(s) | 349, 401, 553, 977 |
| PpuMI | RGGWCCY | 1 cut(s) | 1242 |
| PshBI | ATTAAT | 1 cut(s) | 807 |
| PsiI | TTATAA | 1 cut(s) | 132 |
| Psp5II | RGGWCCY | 1 cut(s) | 1242 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 228, 706 |
| PspOMI | GGGCCC | 1 cut(s) | 704 |
| PspPI | GGNCC | 4 cut(s) | 227, 704, 705, 1242 |
| PspPPI | RGGWCCY | 1 cut(s) | 1242 |
| PvuII | CAGCTG | 1 cut(s) | 1042 |
| RsaI | GTAC | 4 cut(s) | 25, 188, 562, 781 |
| RsaNI | GTAC | 4 cut(s) | 24, 187, 561, 780 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1370 |
| SaqAI | TTAA | 6 cut(s) | 530, 807, 939, 1184, 1448, 1527 |
| SatI | GCNGC | 7 cut(s) | 111, 114, 239, 340, 513, 1377, 1380 |
| Sau3AI | GATC | 5 cut(s) | 259, 784, 861, 978, 1057 |
| Sau96I | GGNCC | 4 cut(s) | 227, 704, 705, 1242 |
| ScaI | AGTACT | 1 cut(s) | 781 |
| SchI | GAGTC | 4 cut(s) | 349, 401, 554, 977 |
| SduI | GDGCHC | 2 cut(s) | 151, 708 |
| SinI | GGWCC | 2 cut(s) | 227, 1242 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1370 |
| SmlI | CTYRAG | 3 cut(s) | 507, 959, 1011 |
| SmoI | CTYRAG | 3 cut(s) | 507, 959, 1011 |
| SsiI | CCGC | 7 cut(s) | 108, 113, 239, 305, 669, 702, 1190 |
| SspI | AATATT | 1 cut(s) | 1396 |
| SspMI | CTAG | 5 cut(s) | 251, 1248, 1266, 1383, 1472 |
| StyI | CCWWGG | 1 cut(s) | 1231 |
| TaiI | ACGT | 4 cut(s) | 220, 536, 599, 977 |
| TaqI | TCGA | 5 cut(s) | 410, 484, 795, 864, 981 |
| TatI | WGTACW | 2 cut(s) | 186, 779 |
| TauI | GCSGC | 2 cut(s) | 116, 241 |
| TfiI | GAWTC | 3 cut(s) | 416, 872, 1036 |
| Tru1I | TTAA | 6 cut(s) | 530, 807, 939, 1184, 1448, 1527 |
| Tru9I | TTAA | 6 cut(s) | 530, 807, 939, 1184, 1448, 1527 |
| TseFI | GTSAC | 2 cut(s) | 895, 1268 |
| TseI | GCWGC | 5 cut(s) | 110, 339, 512, 1376, 1379 |
| Tsp45I | GTSAC | 2 cut(s) | 895, 1268 |
| TspDTI | ATGAA | 6 cut(s) | 74, 299, 690, 896, 917, 1038 |
| VpaK11BI | GGWCC | 2 cut(s) | 227, 1242 |
| VspI | ATTAAT | 1 cut(s) | 807 |
| XapI | RAATTY | 3 cut(s) | 175, 752, 1063 |
| XbaI | TCTAGA | 1 cut(s) | 1471 |
| XceI | RCATGY | 2 cut(s) | 398, 523 |
| XmiI | GTMKAC | 1 cut(s) | 460 |
| XspI | CTAG | 5 cut(s) | 251, 1248, 1266, 1383, 1472 |
| ZrmI | AGTACT | 1 cut(s) | 781 |