FvH4_6g03060

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000230
Physical Location & Seq
Forward (+)
1717344 .. 1719046
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03060.t1

Sequence Viewer

Length: 1329 bp
ATGGCTACCATTTGTTCTTTACCAAGAATTAGCCATACTATAGTCGTCATCACCTTGTTTTTCTTTCATCTTGGATACTACATTGTTACAGCAACAACCAATATCCACAATATCAAGGGTGGATTTAGCGCCCATCTTATCCATAAAAATTCTCCCAAATCACCATTGCCCAAACAAAGAAACAACAAAGTTTCTCGACGGTTGATCGGTCCAGACGTTCTCGAAGCACCAATGAAACTCGATACCAACTTCGAACGTATTATGAAGTTCTCAATGGGAACTCCGCCTATCGATATTCATGCAATTACTGATAGTGGTAGCGATCTAGTATGGACGCAGTGTGAGCCGTGCAATGCTTGTTACAAGACCAAGTTTGGTGTTTTTGACCCAAGAAACTCATCAACTTATAAGAACATTACCTGTCGGTCAGGGGATTGTAGACTACTTGGCGAATCCTATACCTCGAGTTTAGCAAGAGTGTGTAAAAAAGAACCTTCTACAAATTGCGTGTACATGTACAGTTATGCAGACGGGACATACACAGTAGGTAATCTTGGTAGAGAAACAATTTCGTTGAAATCCACTACAGGTAACATTGTGATCCTAAAAGACATTATCTTTGGGTGTGGGACTACGAACAATGACACTTTTAGCACTGGAAATGAAATGGGAGTTATAGGGCTTGGGCGTGGGCCCTTGTCATTTGTCTCTCAAATTGCTCCTTATGTTGGAGGCAGGAGATTCTCTCATTGTTTGGTGCAAGATCCCAATTTAGAAAGCAATATCTATTTCGGGAACGGAAGTGAAGTTTTGGATGAAGGGGTGGTGACAACACCTTTAGTCGATCATACGACCTTTTATTATGTGACGGTATCAGGAATTAGCATCGGAAATGATTTTCTTCCATTTAACTCAAATGGTACCCTGTTAAAGAAAGATAACATGTTGATCGACTCAGGTACACCTTTTCCACGACTACCAAAAGATCTGTTTGACCGGGTGGTAACTAAGCTACAAAAGGCAGTTCCATTAAAGTCAGTCGTAAATAAAATCTATGGGTTGGATACCCTTTGCTTTATTACCACGACGCCTTTAGAATTGCCAAGAATTGCTTTACATTTTGAGGGTGGTGGCGAAGTGCTGTTGAGCAATAAGGTGTTAACAGCTCACGAGAATGGGATGTATTGTCTTGTATTCGTGGGCGGCACTGATGACTTTGGCTATTTTGGGGGCATGCTTCAGACAGATTTATTAATTGGTTTTGATTTGGATAAAAACGTAGTATCCTTCAAGCCAACTGATTGCATAGACTACAACAAAAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

443

Amino Acids

48.65

Weight (kDa)

7.51

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 87 - 266 3e-47 Xylanase inhibitor N-terminal
Asp PF00026 172 - 430 8.3e-07 Eukaryotic aspartyl protease
TAXi_C PF14541 287 - 431 2.8e-19 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
Acc65I GGTACC 1 cut(s) 920
AccB1I GGYRCC 1 cut(s) 920
AccI GTMKAC 1 cut(s) 439
AciI CCGC 2 cut(s) 284, 1203
AclWI GGATC 2 cut(s) 595, 758
AcsI RAATTY 1 cut(s) 148
AcuI CTGAAG 1 cut(s) 1223
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 4 cut(s) 512, 518, 922, 961
AfiI CCNNNNNNNGG 1 cut(s) 728
AflIII ACRYGT 2 cut(s) 513, 942
AgsI TTSAA 2 cut(s) 577, 1291
AluBI AGCT 2 cut(s) 1012, 1166
AluI AGCT 2 cut(s) 1012, 1166
Alw26I GTCTC 1 cut(s) 712
AlwI GGATC 2 cut(s) 595, 758
Ama87I CYCGRG 1 cut(s) 463
AoxI GGCC 1 cut(s) 692
ApaI GGGCCC 1 cut(s) 696
ApoI RAATTY 1 cut(s) 148
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AseI ATTAAT 1 cut(s) 1253
Asp718I GGTACC 1 cut(s) 920
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 209, 692, 693
AsuC2I CCSGG 1 cut(s) 998
AsuHPI GGTGA 3 cut(s) 43, 153, 838
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 463
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 696
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 696
BauI CACGAG 1 cut(s) 1169
BccI CCATC 1 cut(s) 141
BceAI ACGGC 1 cut(s) 331
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciVI GTATCC 3 cut(s) 68, 1057, 1294
BcnI CCSGG 1 cut(s) 998
BcoDI GTCTC 1 cut(s) 712
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 39, 585
BfoI RGCGCY 1 cut(s) 132
BfuI GTATCC 3 cut(s) 68, 1057, 1294
BglII AGATCT 1 cut(s) 985
BisI GCNGC 1 cut(s) 1204
BlsI GCNGC 1 cut(s) 1205
Bme1390I CCNGG 1 cut(s) 998
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 209, 692, 693
BmiI GGNNCC 2 cut(s) 694, 922
BmrFI CCNGG 1 cut(s) 998
BmsI GCATC 1 cut(s) 894
BplI GAGNNNNNCTC 2 cut(s) 730, 762
Bpu14I TTCGAA 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 998
Bsa29I ATCGAT 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 1088
Bsc4I CCNNNNNNNGG 1 cut(s) 728
Bse1I ACTGG 1 cut(s) 661
Bse3DI GCAATG 2 cut(s) 164, 358
BseCI ATCGAT 1 cut(s) 291
BseGI GGATG 2 cut(s) 820, 1185
BseLI CCNNNNNNNGG 1 cut(s) 728
BseMI GCAATG 2 cut(s) 164, 358
BseMII CTCAG 1 cut(s) 969
BseNI ACTGG 1 cut(s) 661
BseSI GKGCMC 1 cut(s) 696
BshFI GGCC 1 cut(s) 694
BshNI GGYRCC 1 cut(s) 920
BshVI ATCGAT 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 463
BsiSI CCGG 1 cut(s) 997
BslFI GGGAC 2 cut(s) 547, 643
BslI CCNNNNNNNGG 1 cut(s) 728
BsmAI GTCTC 1 cut(s) 712
BsmFI GGGAC 2 cut(s) 547, 643
BsnI GGCC 1 cut(s) 694
BsoBI CYCGRG 1 cut(s) 463
Bsp119I TTCGAA 1 cut(s) 252
Bsp120I GGGCCC 1 cut(s) 692
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 2 cut(s) 510, 516
Bsp143I GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
BspACI CCGC 2 cut(s) 284, 1203
BspANI GGCC 1 cut(s) 694
BspCNI CTCAG 1 cut(s) 968
BspDI ATCGAT 1 cut(s) 291
BspLI GGNNCC 2 cut(s) 694, 922
BspPI GGATC 2 cut(s) 595, 758
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 920
BsrDI GCAATG 2 cut(s) 164, 358
BsrGI TGTACA 2 cut(s) 510, 516
BsrI ACTGG 1 cut(s) 661
BssMI GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
BssNI GRCGYC 1 cut(s) 1088
BssSI CACGAG 1 cut(s) 1169
Bst2BI CACGAG 1 cut(s) 1169
Bst4CI ACNGT 4 cut(s) 201, 521, 544, 871
BstACI GRCGYC 1 cut(s) 1088
BstAUI TGTACA 2 cut(s) 510, 516
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 1235
BstDEI CTNAG 2 cut(s) 955, 1008
BstF5I GGATG 2 cut(s) 820, 1185
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 7 cut(s) 207, 325, 603, 766, 847, 951, 988
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNSI RCATGY 3 cut(s) 517, 946, 1237
BstSCI CCNGG 1 cut(s) 996
BstSFI CTRYAG 2 cut(s) 39, 585
BstSLI GKGCMC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 763, 985
BstYI RGATCY 2 cut(s) 763, 985
Bsu15I ATCGAT 1 cut(s) 291
BsuI GTATCC 3 cut(s) 68, 1057, 1294
BsuRI GGCC 1 cut(s) 694
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 2 cut(s) 820, 1185
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 3 cut(s) 344, 654, 1206
Cac8I GCNNGC 1 cut(s) 1235
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 209, 692, 693
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 2 cut(s) 343, 1096
Csp6I GTAC 4 cut(s) 511, 517, 921, 960
CviAII CATG 4 cut(s) 299, 514, 943, 1234
CviJI RGCY 9 cut(s) 5, 33, 346, 682, 694, 1012, 1166, 1221, 1294
CviKI_1 RGCY 9 cut(s) 5, 33, 346, 682, 694, 1012, 1166, 1221, 1294
CviQI GTAC 4 cut(s) 511, 517, 921, 960
DdeI CTNAG 2 cut(s) 955, 1008
DpnI GATC 7 cut(s) 206, 324, 602, 765, 846, 950, 987
DpnII GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
EciI GGCGGA 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 1223
Eco88I CYCGRG 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 693
EcoT38I GRGCYC 1 cut(s) 696
FaeI CATG 4 cut(s) 302, 517, 946, 1237
FalI AAGNNNNNCTT 2 cut(s) 1096, 1128
FaqI GGGAC 2 cut(s) 547, 643
FatI CATG 4 cut(s) 298, 513, 942, 1233
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 1 cut(s) 1204
FokI GGATG 2 cut(s) 827, 1192
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 1 cut(s) 1204
FspBI CTAG 1 cut(s) 326
GlaI GCGC 1 cut(s) 130
GluI GCNGC 1 cut(s) 1204
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 694
HapII CCGG 1 cut(s) 997
HgaI GACGC 2 cut(s) 343, 1096
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 4 cut(s) 302, 517, 946, 1237
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HinfI GANTC 3 cut(s) 452, 741, 953
HpaI GTTAAC 1 cut(s) 1161
HpaII CCGG 1 cut(s) 997
HphI GGTGA 3 cut(s) 43, 153, 838
Hpy166II GTNNAC 4 cut(s) 440, 511, 962, 1161
Hpy188I TCNGA 2 cut(s) 890, 1242
Hpy188III TCNNGA 6 cut(s) 195, 212, 221, 793, 876, 1169
Hpy8I GTNNAC 4 cut(s) 440, 511, 962, 1161
Hpy99I CGWCG 2 cut(s) 201, 1090
HpyAV CCTTC 3 cut(s) 504, 812, 1297
HpyCH4III ACNGT 4 cut(s) 201, 521, 544, 871
HpyCH4IV ACGT 3 cut(s) 216, 256, 1278
HpyCH4V TGCA 5 cut(s) 302, 351, 527, 760, 1305
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 2 cut(s) 955, 1008
HpySE526I ACGT 3 cut(s) 216, 256, 1278
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 4 cut(s) 302, 517, 946, 1237
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 924
KspAI GTTAAC 1 cut(s) 1161
Kzo9I GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
LmnI GCTCC 1 cut(s) 724
LweI GCATC 1 cut(s) 894
MaeI CTAG 1 cut(s) 326
MaeII ACGT 3 cut(s) 216, 256, 1278
MaeIII GTNAC 6 cut(s) 85, 359, 590, 826, 865, 1003
MalI GATC 7 cut(s) 206, 324, 602, 765, 846, 950, 987
MboI GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
MboII GAAGA 1 cut(s) 893
MflI RGATCY 2 cut(s) 763, 985
MhlI GDGCHC 1 cut(s) 696
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 2 cut(s) 709, 1041
MnlI CCTC 3 cut(s) 472, 725, 1117
MseI TTAA 6 cut(s) 909, 929, 1031, 1160, 1253, 1327
MslI CAYNNNNRTG 1 cut(s) 1173
MspI CCGG 1 cut(s) 997
MspR9I CCNGG 1 cut(s) 998
MwoI GCNNNNNNNGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 998
NdeII GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
NlaIII CATG 4 cut(s) 302, 517, 946, 1237
NlaIV GGNNCC 2 cut(s) 694, 922
NmuCI GTSAC 2 cut(s) 826, 865
NspI RCATGY 3 cut(s) 517, 946, 1237
NspV TTCGAA 1 cut(s) 252
PaeI GCATGC 1 cut(s) 1237
PaeR7I CTCGAG 1 cut(s) 463
PciI ACATGT 2 cut(s) 513, 942
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 2 cut(s) 452, 741
PkrI GCNGC 1 cut(s) 1205
PleI GAGTC 1 cut(s) 947
PpsI GAGTC 1 cut(s) 947
PscI ACATGT 2 cut(s) 513, 942
PshBI ATTAAT 1 cut(s) 1253
PsiI TTATAA 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 694, 922
PspOMI GGGCCC 1 cut(s) 692
PspPI GGNCC 3 cut(s) 209, 692, 693
PspXI VCTCGAGB 1 cut(s) 463
PsuI RGATCY 2 cut(s) 763, 985
RsaI GTAC 4 cut(s) 512, 518, 922, 961
RsaNI GTAC 4 cut(s) 511, 517, 921, 960
RseI CAYNNNNRTG 1 cut(s) 1173
SaqAI TTAA 6 cut(s) 909, 929, 1031, 1160, 1253, 1327
SatI GCNGC 1 cut(s) 1204
Sau3AI GATC 7 cut(s) 204, 322, 600, 763, 844, 948, 985
Sau96I GGNCC 3 cut(s) 209, 692, 693
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 1 cut(s) 998
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 1 cut(s) 894
SfcI CTRYAG 2 cut(s) 39, 585
Sfr274I CTCGAG 1 cut(s) 463
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 1173
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SphI GCATGC 1 cut(s) 1237
SsiI CCGC 2 cut(s) 284, 1203
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 996
TaaI ACNGT 4 cut(s) 201, 521, 544, 871
TaiI ACGT 3 cut(s) 219, 259, 1281
TaqI TCGA 8 cut(s) 196, 222, 240, 252, 291, 464, 843, 951
TaqII GACCGA 2 cut(s) 197, 414
TatI WGTACW 2 cut(s) 510, 516
TauI GCSGC 1 cut(s) 1206
TfiI GAWTC 2 cut(s) 452, 741
Tru1I TTAA 6 cut(s) 909, 929, 1031, 1160, 1253, 1327
Tru9I TTAA 6 cut(s) 909, 929, 1031, 1160, 1253, 1327
TscAI CASTG 3 cut(s) 344, 661, 1213
TseFI GTSAC 2 cut(s) 826, 865
Tsp45I GTSAC 2 cut(s) 826, 865
TspDTI ATGAA 6 cut(s) 56, 248, 278, 287, 678, 831
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 3 cut(s) 344, 661, 1213
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 1253
XapI RAATTY 1 cut(s) 148
XceI RCATGY 3 cut(s) 517, 946, 1237
XhoI CTCGAG 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 439
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.