pycom12g22170

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000230
Physical Location & Seq
Reverse (-)
23179329 .. 23183538
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g22170.1

Sequence Viewer

Length: 2697 bp
ATGGATGAAAGTACCCCACAATCAAAACTAAGGCGTGACAAAGAGGGTGGTGAGGGTGCACAACTTATGAAGCTCTCTCTTGGAACTCCACCCTATGAGATTTATGCAGTGACGGATACAAGAAGTATGATGTTGTGGACGCAATGTGAGCCGTGTCCGAATTGCTACAAGCAGAAAAATCCCAAGTTTGATCCGAAAAAGTCCCCATCATACGCAACACTTCCATGTACTGCACAAGAATGTAGTCATGCTAATGACACTGGCTACAGCTCATGCTCAAAGGATGATCAAAAGGTCTGCAACTACAATTACACTTACATGGATGATTCAATAACGCAGGGGGTGGTGTCAAAAGAGACAATCACATTCGGATCCAGCTCCGGGAAGCCAGTTTCGTTCAAAGATGTTGTGATAGGTTGTGGACACAACAATACTGGGAAAACCTTTGGCAAGAAATTCTCACATTGTCTCGTGCCCCTTGATCCGGATCACCCCAATGATGCAAGCATCATGAGTTTCGGAAGGGGAAGTGAGGTTTCGGGTGAAGGTGTAGTTTCGACTCCATTAATCACCAAACATTGCAAAACCCAATATTTTGTCACGGTTGAAGGAATCAGCGTCGGAGAACAGTTTGTGCCTTTTAACTCATCAGGTTCGGTTTCAAAAGGGAACATGTATCTCGACTCGGGTACACAAGTGACAATGCTGCCACAAGATTTTTACGGCAGATCGGTAGAACAAGTAAAGAAGACGATGAATTCGTCACTAAAACCAGTCGAAGTAAATGACGAGTCTGGAACTTTGCTTTGCTACAACACCACGGAGAATCTCCAAGCACCATTATTCACGGTTCATTTCTACGGCGGTGCTAAATTGCAGCTGTCACCGGCACAAACTTTCTATCAAAACAAACAACATAAACTACACTGCTTCGGAACGCTTAACTCGAGTCATACCATAGGTGAAGGAATTGGTCTCTATGGAAGCTATGCTCAGTCAAATTTTTTGGTCGGTTTTGATCTTGAAAAGATGTTGGTCTCCTTCAAGGCAGCTGATTGCAGAAAAACTAGCAGTACTAGTAGTTCTAGTCCTAATAGTGCTTTTGCTATTCATATTTCTTATTTTTTCAACTACGTTTTCAGTTTTATTTTGATTGTAATTTTGAGAATTAATTACTCTCCTATGGCAGCTGCAGGCACTACTGCTTTATACAATCATATAATTTTGATCACTGTTCTAATTTATCTATGCCATAATTCAGCTACATCTAGAGTAGCAGCATGCAGTGGTATTAGTACTAATGGCGGGTTTAGCGTACCACTTACTCGCCGAAACTCCCCAAATTCGCCATTATACAATCGTAACAAAGTTTTTCGGCATCTCATGGGACCAGCCGGTGACCAAAACAGCCCACAATCAGAAGTAACACGGGACACCATAGACGGTGAGGGTGCACACGTTATGAAACTCTCAATTGGAACTCCGCCATTTGATATTTACACCGTCGCTGATACTGGCAGCTCGCTCATATGGACGCAATGCGAGCCATGTCCAGGATGCTACAAGCAGAAAAAACCCATGTTCGACCCGCGGAAATCCTCGACGTATCATATCATCCCATGTTATGCGCTAGAATGTAATGCTACTATTGATGATTTCTCATGCTCAAGAAGTGGTGATCAAAATGTTTGCAGTTACAATTACGTTTACATGGACCAGTCGAAGACACATGGAGTAGTCGCAAAAGACACGATTACCTTGGCGTCCGGATCAGGCAAGCCGGTTTCGTTCAAAGATGTTGTGTTTGGTTGTGGACATAACAACACTGGGGAGAATTTTTCTGAAAATCAAGTGGGAATGGTAGGGCTTGGCCTTGGGAACACATCACTTGTTTCTCAAATCGCTCCCTACGTCGGAGGCAGAAAGTTCTCCCATTGTCTCGTGCCGTTCGACCCTGATCACACGAATGCTGCAGGCACGTTAAGGTTTGGGAACGGAAGTGAGGTTACTGGTGAAGGAGCGGTTTCGACGCCTTTGATCATTAAGGATCAAAACGACAGGTACGCGTACCAATATCATGTGACAGTTGAAGGAATCAGTGTTGGAAACACATTTGTGCCTTTTGACTCATCGGGGTCGGTTTCGAAAGGGAACATGTTTCTTGACTCTGGAACACAAGTGACAGTACTACCACAGGATTTCTATGGCCAATTGATGGAACAAGTAAAGAAGGCAGTTGATCTGAAACCGGTTCTAGTAGGTGACCCGAAAAACGGAACTCGGTTTTGCTACAATACAAGCACGTTGGATCATCTCAAAGGAGCGACATTGACAGTGCATTTTGACGGCGGTGGTAAAGTGCAATTAACACCGGAACAAGCCTTCTTCCATACCGAAGATGATGGATATAAGCTGTTGTGTTCTATGTTGATGAATGCCACCGAAGTTGCAAGTGAAGACCTTAATGTTGCTATTTATGGAAGCAACGCTCAGTCGAATTTCTGGGTCGGTTTCGACCTTGAAAGAATGACGGTCTCCTTCAAGCCAACTGATTGCAGAAAGAGTAGTGGAGATAGAAAGAGTAGTGGAGATGGAAAGAGTAGTGGCGATGGAAAGAGTAGTTCTACTGTCATTCAAATTTCCTATTTTTACACCTGCCTTATTAATTTTCTTTTATTTGTAATTTTTTCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

899

Amino Acids

97.8

Weight (kDa)

6.45

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 22 - 149 1.2e-30 Xylanase inhibitor N-terminal
Asp PF00026 172 - 348 2.9e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 197 - 349 2.9e-25 Xylanase inhibitor C-terminal
TAXi_N PF14543 487 - 665 4.9e-49 Xylanase inhibitor N-terminal
Asp PF00026 560 - 847 6.1e-10 Eukaryotic aspartyl protease
TAXi_C PF14541 691 - 848 1.2e-20 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2663
Acc36I ACCTGC 1 cut(s) 2663
AccB7I CCANNNNNTGG 1 cut(s) 2215
AccBSI CCGCTC 1 cut(s) 2021
AccII CGCG 2 cut(s) 1591, 2066
AccIII TCCGGA 2 cut(s) 484, 1766
AciI CCGC 7 cut(s) 864, 1305, 1484, 1589, 1591, 2021, 2349
AclWI GGATC 8 cut(s) 185, 366, 379, 476, 495, 1777, 2055, 2316
AcoI YGGCCR 1 cut(s) 2206
AcsI RAATTY 7 cut(s) 455, 757, 1000, 1342, 1834, 2497, 2637
AcyI GRCGYC 2 cut(s) 1763, 2030
AfaI GTAC 9 cut(s) 13, 229, 691, 1075, 1297, 1317, 2063, 2069, 2187
AfiI CCNNNNNNNGG 6 cut(s) 381, 484, 1553, 1913, 2215, 2273
AflIII ACRYGT 4 cut(s) 672, 1456, 2064, 2154
AgeI ACCGGT 1 cut(s) 2248
AhlI ACTAGT 1 cut(s) 1076
AjnI CCWGG 1 cut(s) 1552
AleI CACNNNNGTG 1 cut(s) 2114
Alw21I GWGCWC 2 cut(s) 61, 1456
Alw26I GTCTC 6 cut(s) 350, 473, 980, 1042, 1943, 2539
Alw44I GTGCAC 2 cut(s) 57, 1452
AlwI GGATC 8 cut(s) 185, 366, 379, 476, 495, 1777, 2055, 2316
Ama87I CYCGRG 2 cut(s) 685, 946
Aor13HI TCCGGA 2 cut(s) 484, 1766
AoxI GGCC 2 cut(s) 1870, 2206
ApaLI GTGCAC 2 cut(s) 57, 1452
ApeKI GCWGC 8 cut(s) 706, 877, 1049, 1187, 1190, 1277, 1518, 1970
ApoI RAATTY 7 cut(s) 455, 757, 1000, 1342, 1834, 2497, 2637
ArsI GACNNNNNNTTYG 2 cut(s) 742, 774
AseI ATTAAT 3 cut(s) 566, 1170, 2664
AsiGI ACCGGT 1 cut(s) 2248
AspLEI GCGC 1 cut(s) 1630
AspS9I GGNCC 2 cut(s) 1388, 1714
AsuC2I CCSGG 1 cut(s) 382
AsuII TTCGAA 1 cut(s) 2144
AvaI CYCGRG 2 cut(s) 685, 946
AvaII GGWCC 2 cut(s) 1388, 1714
BaeGI GKGCMC 3 cut(s) 61, 477, 1456
BaeI ACNNNNGTAYC 2 cut(s) 681, 714
BalI TGGCCA 1 cut(s) 2208
BamHI GGATCC 1 cut(s) 371
BarI GAAGNNNNNNTAC 2 cut(s) 1990, 2022
BauI CACGAG 2 cut(s) 470, 1940
BbsI GAAGAC 3 cut(s) 755, 1730, 2463
Bbv12I GWGCWC 2 cut(s) 61, 1456
BbvI GCAGC 8 cut(s) 693, 889, 1061, 1177, 1199, 1289, 1530, 1957
BccI CCATC 5 cut(s) 214, 2209, 2396, 2585, 2603
BceAI ACGGC 5 cut(s) 136, 739, 877, 1930, 2362
BciT130I CCWGG 1 cut(s) 1554
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 5 cut(s) 286, 1227, 1678, 1957, 2037
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 6 cut(s) 350, 473, 980, 1042, 1943, 2539
BcuI ACTAGT 1 cut(s) 1076
BfaI CTAG 6 cut(s) 1068, 1077, 1086, 1269, 1631, 2255
BfmI CTRYAG 3 cut(s) 265, 1191, 1971
BfuAI ACCTGC 1 cut(s) 2663
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 8 cut(s) 707, 878, 1050, 1188, 1191, 1278, 1519, 1971
BlsI GCNGC 8 cut(s) 708, 879, 1051, 1189, 1192, 1279, 1520, 1972
BmcAI AGTACT 3 cut(s) 1075, 1297, 2187
Bme1390I CCNGG 2 cut(s) 382, 1554
Bme18I GGWCC 2 cut(s) 1388, 1714
BmeT110I CYCGRG 2 cut(s) 685, 946
BmgT120I GGNCC 2 cut(s) 1388, 1714
BmiI GGNNCC 2 cut(s) 373, 1389
BmrFI CCNGG 2 cut(s) 382, 1554
BmrI ACTGGG 2 cut(s) 444, 1836
BmsI GCATC 4 cut(s) 490, 516, 1387, 1547
BmuI ACTGGG 2 cut(s) 444, 1836
BpiI GAAGAC 3 cut(s) 755, 1730, 2463
Bpu14I TTCGAA 1 cut(s) 2144
BpuEI CTTGAG 1 cut(s) 1651
BpuMI CCSGG 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 2 cut(s) 1763, 2030
BsaI GGTCTC 3 cut(s) 980, 1042, 2539
BsaJI CCNNGG 4 cut(s) 819, 1589, 1758, 1873
BsaWI WCCGGW 4 cut(s) 484, 1766, 2248, 2371
Bsc4I CCNNNNNNNGG 6 cut(s) 381, 484, 1553, 1913, 2215, 2273
Bse118I RCCGGY 4 cut(s) 886, 1394, 1780, 2248
Bse1I ACTGG 8 cut(s) 265, 389, 439, 773, 1519, 1717, 1831, 2014
Bse3DI GCAATG 3 cut(s) 149, 577, 1544
Bse8I GATNNNNATC 1 cut(s) 486
BseAI TCCGGA 2 cut(s) 484, 1766
BseBI CCWGG 1 cut(s) 1554
BseDI CCNNGG 4 cut(s) 819, 1589, 1758, 1873
BseGI GGATG 5 cut(s) 10, 289, 328, 1562, 1614
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 6 cut(s) 381, 484, 1553, 1913, 2215, 2273
BseMI GCAATG 3 cut(s) 149, 577, 1544
BseMII CTCAG 2 cut(s) 1007, 2504
BseNI ACTGG 8 cut(s) 265, 389, 439, 773, 1519, 1717, 1831, 2014
BseSI GKGCMC 3 cut(s) 61, 477, 1456
BseXI GCAGC 8 cut(s) 693, 889, 1061, 1177, 1199, 1289, 1530, 1957
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 2 cut(s) 1591, 2066
BshFI GGCC 2 cut(s) 1872, 2208
BshTI ACCGGT 1 cut(s) 2248
BsiHKAI GWGCWC 2 cut(s) 61, 1456
BsiHKCI CYCGRG 2 cut(s) 685, 946
BsiSI CCGG 8 cut(s) 381, 485, 887, 1395, 1767, 1781, 2249, 2372
BslFI GGGAC 3 cut(s) 187, 1401, 1445
BslI CCNNNNNNNGG 6 cut(s) 381, 484, 1553, 1913, 2215, 2273
BsmAI GTCTC 6 cut(s) 350, 473, 980, 1042, 1943, 2539
BsmFI GGGAC 3 cut(s) 187, 1401, 1445
BsmI GAATGC 2 cut(s) 1972, 2440
BsnI GGCC 2 cut(s) 1872, 2208
Bso31I GGTCTC 3 cut(s) 980, 1042, 2539
BsoBI CYCGRG 2 cut(s) 685, 946
Bsp119I TTCGAA 1 cut(s) 2144
Bsp1286I GDGCHC 3 cut(s) 61, 477, 1456
Bsp13I TCCGGA 2 cut(s) 484, 1766
BspACI CCGC 7 cut(s) 864, 1305, 1484, 1589, 1591, 2021, 2349
BspANI GGCC 2 cut(s) 1872, 2208
BspCNI CTCAG 2 cut(s) 1006, 2503
BspEI TCCGGA 2 cut(s) 484, 1766
BspFNI CGCG 2 cut(s) 1591, 2066
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 373, 1389
BspMAI CTGCAG 2 cut(s) 1195, 1975
BspMI ACCTGC 1 cut(s) 2663
BspPI GGATC 8 cut(s) 185, 366, 379, 476, 495, 1777, 2055, 2316
BspT104I TTCGAA 1 cut(s) 2144
BspTNI GGTCTC 3 cut(s) 980, 1042, 2539
BsrBI CCGCTC 1 cut(s) 2021
BsrDI GCAATG 3 cut(s) 149, 577, 1544
BsrFI RCCGGY 4 cut(s) 886, 1394, 1780, 2248
BsrI ACTGG 8 cut(s) 265, 389, 439, 773, 1519, 1717, 1831, 2014
BssAI RCCGGY 4 cut(s) 886, 1394, 1780, 2248
BssECI CCNNGG 4 cut(s) 819, 1589, 1758, 1873
BssNI GRCGYC 2 cut(s) 1763, 2030
BssSI CACGAG 2 cut(s) 470, 1940
BssT1I CCWWGG 2 cut(s) 1758, 1873
Bst2BI CACGAG 2 cut(s) 470, 1940
Bst2UI CCWGG 1 cut(s) 1554
BstACI GRCGYC 2 cut(s) 1763, 2030
BstBI TTCGAA 1 cut(s) 2144
BstC8I GCNNGC 7 cut(s) 505, 1195, 1282, 1523, 1544, 1778, 1975
BstDEI CTNAG 3 cut(s) 29, 993, 2490
BstDSI CCRYGG 2 cut(s) 819, 1589
BstEII GGTNACC 2 cut(s) 1397, 2261
BstF5I GGATG 5 cut(s) 10, 289, 328, 1562, 1614
BstFNI CGCG 2 cut(s) 1591, 2066
BstHHI GCGC 1 cut(s) 1630
BstMAI GTCTC 6 cut(s) 350, 473, 980, 1042, 1943, 2539
BstMWI GCNNNNNNNGC 3 cut(s) 148, 1311, 1543
BstNI CCWGG 1 cut(s) 1554
BstNSI RCATGY 3 cut(s) 676, 1284, 2158
BstPI GGTNACC 2 cut(s) 1397, 2261
BstSCI CCNGG 2 cut(s) 380, 1552
BstSFI CTRYAG 3 cut(s) 265, 1191, 1971
BstSLI GKGCMC 3 cut(s) 61, 477, 1456
BstUI CGCG 2 cut(s) 1591, 2066
BstV1I GCAGC 8 cut(s) 693, 889, 1061, 1177, 1199, 1289, 1530, 1957
BstV2I GAAGAC 3 cut(s) 755, 1730, 2463
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 1872, 2208
BtgI CCRYGG 2 cut(s) 819, 1589
BtgZI GCGATG 1 cut(s) 2622
BtsCI GGATG 5 cut(s) 10, 289, 328, 1562, 1614
BtsI GCAGTG 3 cut(s) 114, 925, 1291
BtsIMutI CAGTG 8 cut(s) 114, 258, 925, 1230, 1291, 1824, 2104, 2340
BveI ACCTGC 1 cut(s) 2663
Cac8I GCNNGC 7 cut(s) 505, 1195, 1282, 1523, 1544, 1778, 1975
CciI TCATGA 1 cut(s) 510
CfoI GCGC 1 cut(s) 1630
Cfr10I RCCGGY 4 cut(s) 886, 1394, 1780, 2248
Cfr13I GGNCC 2 cut(s) 1388, 1714
Cfr42I CCGCGG 1 cut(s) 1592
CseI GACGC 5 cut(s) 148, 607, 1543, 1752, 2038
Csp6I GTAC 9 cut(s) 12, 228, 690, 1074, 1296, 1316, 2062, 2068, 2186
CspAI ACCGGT 1 cut(s) 2248
CspCI CAANNNNNGTGG 4 cut(s) 28, 63, 2428, 2463
CviQI GTAC 9 cut(s) 12, 228, 690, 1074, 1296, 1316, 2062, 2068, 2186
DdeI CTNAG 3 cut(s) 29, 993, 2490
EaeI YGGCCR 1 cut(s) 2206
EciI GGCGGA 1 cut(s) 1473
Eco130I CCWWGG 2 cut(s) 1758, 1873
Eco31I GGTCTC 3 cut(s) 980, 1042, 2539
Eco47I GGWCC 2 cut(s) 1388, 1714
Eco88I CYCGRG 2 cut(s) 685, 946
Eco91I GGTNACC 2 cut(s) 1397, 2261
EcoO65I GGTNACC 2 cut(s) 1397, 2261
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 1552
EcoT14I CCWWGG 2 cut(s) 1758, 1873
ErhI CCWWGG 2 cut(s) 1758, 1873
FaqI GGGAC 3 cut(s) 187, 1401, 1445
FauI CCCGC 2 cut(s) 1298, 1596
FauNDI CATATG 1 cut(s) 1529
FbaI TGATCA 5 cut(s) 286, 1227, 1678, 1957, 2037
Fnu4HI GCNGC 8 cut(s) 707, 878, 1050, 1188, 1191, 1278, 1519, 1971
FokI GGATG 5 cut(s) 17, 296, 335, 1569, 1601
Fsp4HI GCNGC 8 cut(s) 707, 878, 1050, 1188, 1191, 1278, 1519, 1971
FspBI CTAG 6 cut(s) 1068, 1077, 1086, 1269, 1631, 2255
GlaI GCGC 1 cut(s) 1629
GluI GCNGC 8 cut(s) 707, 878, 1050, 1188, 1191, 1278, 1519, 1971
HaeIII GGCC 2 cut(s) 1872, 2208
HapII CCGG 8 cut(s) 381, 485, 887, 1395, 1767, 1781, 2249, 2372
HgaI GACGC 5 cut(s) 148, 607, 1543, 1752, 2038
HhaI GCGC 1 cut(s) 1630
Hin1I GRCGYC 2 cut(s) 1763, 2030
Hin6I GCGC 1 cut(s) 1628
HinP1I GCGC 1 cut(s) 1628
HpaII CCGG 8 cut(s) 381, 485, 887, 1395, 1767, 1781, 2249, 2372
Hpy166II GTNNAC 7 cut(s) 59, 138, 422, 692, 1454, 1708, 1814
Hpy8I GTNNAC 7 cut(s) 59, 138, 422, 692, 1454, 1708, 1814
Hpy99I CGWCG 5 cut(s) 623, 1508, 1606, 1916, 2032
HpyCH4IV ACGT 7 cut(s) 1134, 1458, 1604, 1704, 1911, 1979, 2303
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 1311, 1543
HpyF3I CTNAG 3 cut(s) 29, 993, 2490
HpySE526I ACGT 7 cut(s) 1134, 1458, 1604, 1704, 1911, 1979, 2303
Hsp92I GRCGYC 2 cut(s) 1763, 2030
HspAI GCGC 1 cut(s) 1628
Kpn2I TCCGGA 2 cut(s) 484, 1766
Ksp22I TGATCA 5 cut(s) 286, 1227, 1678, 1957, 2037
KspI CCGCGG 1 cut(s) 1592
LmnI GCTCC 4 cut(s) 383, 1909, 2018, 2321
Lsp1109I GCAGC 8 cut(s) 693, 889, 1061, 1177, 1199, 1289, 1530, 1957
LweI GCATC 4 cut(s) 490, 516, 1387, 1547
MaeI CTAG 6 cut(s) 1068, 1077, 1086, 1269, 1631, 2255
MaeII ACGT 7 cut(s) 1134, 1458, 1604, 1704, 1911, 1979, 2303
MbiI CCGCTC 1 cut(s) 2021
MboII GAAGA 5 cut(s) 760, 1735, 2377, 2408, 2468
MfeI CAATTG 2 cut(s) 1473, 2210
MflI RGATCY 1 cut(s) 371
MhlI GDGCHC 3 cut(s) 61, 477, 1456
MlsI TGGCCA 1 cut(s) 2208
MluI ACGCGT 1 cut(s) 2064
MluNI TGGCCA 1 cut(s) 2208
MlyI GAGTC 6 cut(s) 553, 677, 800, 958, 2120, 2159
MmeI TCCRAC 4 cut(s) 601, 1894, 2083, 2286
MnlI CCTC 7 cut(s) 37, 46, 526, 1441, 1609, 1910, 1996
Mox20I TGGCCA 1 cut(s) 2208
MroI TCCGGA 2 cut(s) 484, 1766
MscI TGGCCA 1 cut(s) 2208
MseI TTAA 9 cut(s) 566, 642, 942, 1170, 1982, 2043, 2366, 2463, 2664
MslI CAYNNNNRTG 7 cut(s) 223, 238, 252, 317, 495, 1965, 2114
Msp20I TGGCCA 1 cut(s) 2208
MspA1I CMGCKG 4 cut(s) 880, 1052, 1190, 1591
MspI CCGG 8 cut(s) 381, 485, 887, 1395, 1767, 1781, 2249, 2372
MspR9I CCNGG 2 cut(s) 382, 1554
MunI CAATTG 2 cut(s) 1473, 2210
Mva1269I GAATGC 2 cut(s) 1972, 2440
MvaI CCWGG 1 cut(s) 1554
MvnI CGCG 2 cut(s) 1591, 2066
MwoI GCNNNNNNNGC 3 cut(s) 148, 1311, 1543
NciI CCSGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 1529
NlaIV GGNNCC 2 cut(s) 373, 1389
NspI RCATGY 3 cut(s) 676, 1284, 2158
NspV TTCGAA 1 cut(s) 2144
OliI CACNNNNGTG 1 cut(s) 2114
PaeI GCATGC 1 cut(s) 1284
PaeR7I CTCGAG 1 cut(s) 946
PagI TCATGA 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 2663
PciI ACATGT 2 cut(s) 672, 2154
PcsI WCGNNNNNNNCGW 4 cut(s) 758, 944, 1908, 1947
PctI GAATGC 2 cut(s) 1972, 2440
PfeI GAWTC 4 cut(s) 326, 612, 826, 2094
PflMI CCANNNNNTGG 1 cut(s) 2215
PfoI TCCNGGA 2 cut(s) 380, 1552
PinAI ACCGGT 1 cut(s) 2248
PkrI GCNGC 8 cut(s) 708, 879, 1051, 1189, 1192, 1279, 1520, 1972
PleI GAGTC 6 cut(s) 553, 677, 799, 957, 2120, 2159
PpsI GAGTC 6 cut(s) 553, 677, 799, 957, 2120, 2159
PscI ACATGT 2 cut(s) 672, 2154
PshBI ATTAAT 3 cut(s) 566, 1170, 2664
Psp6I CCWGG 1 cut(s) 1552
PspEI GGTNACC 2 cut(s) 1397, 2261
PspGI CCWGG 1 cut(s) 1552
PspN4I GGNNCC 2 cut(s) 373, 1389
PspPI GGNCC 2 cut(s) 1388, 1714
PspXI VCTCGAGB 1 cut(s) 946
PsrI GAACNNNNNNTAC 2 cut(s) 1066, 1098
PstI CTGCAG 2 cut(s) 1195, 1975
PsuI RGATCY 1 cut(s) 371
PvuII CAGCTG 3 cut(s) 880, 1052, 1190
RsaI GTAC 9 cut(s) 13, 229, 691, 1075, 1297, 1317, 2063, 2069, 2187
RsaNI GTAC 9 cut(s) 12, 228, 690, 1074, 1296, 1316, 2062, 2068, 2186
RseI CAYNNNNRTG 7 cut(s) 223, 238, 252, 317, 495, 1965, 2114
SacII CCGCGG 1 cut(s) 1592
SaqAI TTAA 9 cut(s) 566, 642, 942, 1170, 1982, 2043, 2366, 2463, 2664
SatI GCNGC 8 cut(s) 707, 878, 1050, 1188, 1191, 1278, 1519, 1971
Sau96I GGNCC 2 cut(s) 1388, 1714
ScaI AGTACT 3 cut(s) 1075, 1297, 2187
SchI GAGTC 6 cut(s) 553, 677, 800, 958, 2120, 2159
ScrFI CCNGG 2 cut(s) 382, 1554
SduI GDGCHC 3 cut(s) 61, 477, 1456
SfaNI GCATC 4 cut(s) 490, 516, 1387, 1547
SfcI CTRYAG 3 cut(s) 265, 1191, 1971
Sfr274I CTCGAG 1 cut(s) 946
Sfr303I CCGCGG 1 cut(s) 1592
SfuI TTCGAA 1 cut(s) 2144
SgrBI CCGCGG 1 cut(s) 1592
SinI GGWCC 2 cut(s) 1388, 1714
SlaI CTCGAG 1 cut(s) 946
SmiMI CAYNNNNRTG 7 cut(s) 223, 238, 252, 317, 495, 1965, 2114
SmlI CTYRAG 2 cut(s) 946, 1666
SmoI CTYRAG 2 cut(s) 946, 1666
SpeI ACTAGT 1 cut(s) 1076
SphI GCATGC 1 cut(s) 1284
SsiI CCGC 7 cut(s) 864, 1305, 1484, 1589, 1591, 2021, 2349
SspI AATATT 1 cut(s) 593
SspMI CTAG 6 cut(s) 1068, 1077, 1086, 1269, 1631, 2255
StyD4I CCNGG 2 cut(s) 380, 1552
StyI CCWWGG 2 cut(s) 1758, 1873
TaiI ACGT 7 cut(s) 1137, 1461, 1607, 1707, 1914, 1982, 2306
TatI WGTACW 4 cut(s) 227, 1073, 1295, 2185
TfiI GAWTC 4 cut(s) 326, 612, 826, 2094
Tru1I TTAA 9 cut(s) 566, 642, 942, 1170, 1982, 2043, 2366, 2463, 2664
Tru9I TTAA 9 cut(s) 566, 642, 942, 1170, 1982, 2043, 2366, 2463, 2664
TscAI CASTG 8 cut(s) 114, 265, 932, 1237, 1291, 1831, 2104, 2340
TseI GCWGC 8 cut(s) 706, 877, 1049, 1187, 1190, 1277, 1518, 1970
TspDTI ATGAA 8 cut(s) 21, 83, 770, 842, 1100, 1478, 2447, 2679
TspGWI ACGGA 4 cut(s) 128, 836, 2010, 2289
TspRI CASTG 8 cut(s) 114, 265, 932, 1237, 1291, 1831, 2104, 2340
Van91I CCANNNNNTGG 1 cut(s) 2215
VneI GTGCAC 2 cut(s) 57, 1452
VpaK11BI GGWCC 2 cut(s) 1388, 1714
VspI ATTAAT 3 cut(s) 566, 1170, 2664
XapI RAATTY 7 cut(s) 455, 757, 1000, 1342, 1834, 2497, 2637
XbaI TCTAGA 1 cut(s) 1268
XceI RCATGY 3 cut(s) 676, 1284, 2158
XhoI CTCGAG 1 cut(s) 946
XspI CTAG 6 cut(s) 1068, 1077, 1086, 1269, 1631, 2255
ZrmI AGTACT 3 cut(s) 1075, 1297, 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.