MD12G1242600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000230
Physical Location & Seq
Reverse (-)
31377047 .. 31378540
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1242600.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGGCAGCTGCAGGCACTACTACTTTATACAATCATATGATTTTGATCACTTTTCTAATTTATCTATGCCATAATTCAGCTACATCTAGAGTAGCAGCATGCAGTGGTATTAGTACTAATGGCGGGTTTAGCGTACCACTTACTCGCCGAAACTCCCCAAATTCGTCATTATACAATCGTAACAAAGTTTTTCGGCGTCTCATGGGGCCATCCGGTGACCAAAACACCCCACAATCAGAAGTAACACGGGACACCATAGACGGTGAGGGTGCACACGTTATGAAACTCTCAATTGGAACTCCGCCATTTGATATTTACACCGTGGCTGATACTGGCAGCTCGCTCATATGGACGCAATGCGAGCCGTGCCCAGGATGCTACAAGCAGAAAAAACCCATGTTCGACCCGCGGAAATCCTCCACGTATCATATCATCCCATGTTATGCGCCAGAATATAATGCTACAATTGATGATTTCTCGTGCTCAAGAAGTGGTGATCAAAATGTTTGCAGTTACAATTACGTTTACATGGACCAGTCGAAGACACAAGGAGTAGTCGCAAAAGACACGATTACGTTGGAGTCCGGATCAGGGAAGCCGGTTTCGTTCAAAGAGGTTGTGTTTGGTTGTGGACATAACAACACCGGGGAGAATTTTTCTGAAAATCTTATGGGAATGGTAGGGCTTGGCCTTGGGAACACATCACTTGTTTCTCAAATCGCTCCCTACGTCGGAGGCAGAAAGTTCTCCCATTGTCTCGTGCCATTCGATCCTGATCACCCGAATGCTGCAGGCACGTTGAGCTTTGGGAACGGAAGTGAGGTTACTGGTGAAGGAGCGGTTTCGACGCCTTTGATCATTAAAGATCAAAACGACAGGTACGCGTACCAATATCATGTGACAGTTGAAGGAATCAGTGTTGGAACCACATTTGTGCCTTTTGACTCATCGGGGTCGGTTTCGAAAGGGAACATGTTTCTTGACTCTGGAACACAAGTGACAGTGCTACCACAGGATTTCTATGGCCAATTGATGGAACAAGTAAAGAAGGCAGTTGATCTGAAACCGGTTCTAGTGGGTGACCCGAAAAACGGAACTCGGTTTTGCTACAATACAAGCACGTTGGATCATCTCAAAGGAGCGACATTGACAGTGCATTTTGAAGGCGGTGGTAAAGTGAAATTAACACCGGAACAAGCCTTCTTCCATACCGAAGATGATGGATATAAGCTGTTGTGTTCTATGTTGATGAATGCCACCGAAGTTGCAAGTGAAGACGTTAATGTTGCTATTTATGGAAGCAACGCTCAGTCGAATTTCTGGGTCGGTTTCGACCTTGAAAGAATGATGGTCTCCTTCAAGCCAACCGATTGCAGGAGTGGTGGCGATGGAAAGAGTACTGGCGATGGAAAGAGTAGTTCTACTGTCATTCACTTTTCCTATTTTTACACTTGCCTTTTTAATTTTCTTTTAATTTTCATTTTCTCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

498

Amino Acids

54.0

Weight (kDa)

5.84

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 93 - 271 2e-49 Xylanase inhibitor N-terminal
Asp PF00026 167 - 453 4.9e-10 Eukaryotic aspartyl protease
TAXi_C PF14541 297 - 454 2.4e-21 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccBSI CCGCTC 1 cut(s) 839
AccII CGCG 2 cut(s) 409, 884
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 6 cut(s) 123, 302, 407, 409, 839, 1167
AclWI GGATC 3 cut(s) 595, 764, 1134
AcoI YGGCCR 1 cut(s) 1024
AcsI RAATTY 3 cut(s) 160, 652, 1315
AcyI GRCGYC 2 cut(s) 196, 848
AfaI GTAC 5 cut(s) 115, 135, 881, 887, 1399
AfiI CCNNNNNNNGG 6 cut(s) 371, 591, 731, 1033, 1091, 1374
AflIII ACRYGT 3 cut(s) 274, 882, 972
AgeI ACCGGT 1 cut(s) 1066
AgsI TTSAA 5 cut(s) 610, 908, 1163, 1340, 1360
AjnI CCWGG 1 cut(s) 370
AleI CACNNNNGTG 1 cut(s) 932
AluBI AGCT 5 cut(s) 8, 80, 339, 804, 1231
AluI AGCT 5 cut(s) 8, 80, 339, 804, 1231
Alw21I GWGCWC 2 cut(s) 274, 485
Alw26I GTCTC 3 cut(s) 203, 761, 1357
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 3 cut(s) 595, 764, 1134
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 3 cut(s) 206, 688, 1024
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 5 cut(s) 5, 8, 95, 336, 788
ApoI RAATTY 3 cut(s) 160, 652, 1315
AsiGI ACCGGT 1 cut(s) 1066
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 2 cut(s) 206, 532
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 6 cut(s) 227, 275, 506, 770, 842, 1091
AsuII TTCGAA 1 cut(s) 962
AvaII GGWCC 1 cut(s) 532
BaeGI GKGCMC 2 cut(s) 274, 371
BalI TGGCCA 1 cut(s) 1026
BarI GAAGNNNNNNTAC 2 cut(s) 808, 840
BauI CACGAG 2 cut(s) 478, 758
BbsI GAAGAC 2 cut(s) 548, 1281
Bbv12I GWGCWC 2 cut(s) 274, 485
BbvI GCAGC 4 cut(s) 17, 107, 348, 775
BccI CCATC 6 cut(s) 217, 1027, 1214, 1342, 1382, 1400
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 4 cut(s) 45, 496, 775, 855
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 3 cut(s) 203, 761, 1357
BfaI CTAG 2 cut(s) 87, 1073
BfmI CTRYAG 2 cut(s) 9, 789
BisI GCNGC 5 cut(s) 6, 9, 96, 337, 789
BlsI GCNGC 5 cut(s) 7, 10, 97, 338, 790
BmcAI AGTACT 2 cut(s) 115, 1399
Bme1390I CCNGG 2 cut(s) 372, 646
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 2 cut(s) 206, 532
BmiI GGNNCC 2 cut(s) 207, 925
BmrFI CCNGG 2 cut(s) 372, 646
BmsI GCATC 1 cut(s) 365
BpiI GAAGAC 2 cut(s) 548, 1281
Bpu14I TTCGAA 1 cut(s) 962
BpuEI CTTGAG 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 646
BsaAI YACGTR 1 cut(s) 423
BsaBI GATNNNNATC 2 cut(s) 44, 774
BsaHI GRCGYC 2 cut(s) 196, 848
BsaI GGTCTC 1 cut(s) 1357
BsaJI CCNNGG 5 cut(s) 321, 370, 407, 645, 691
BsaWI WCCGGW 4 cut(s) 212, 584, 1066, 1189
Bsc4I CCNNNNNNNGG 6 cut(s) 371, 591, 731, 1033, 1091, 1374
Bse118I RCCGGY 2 cut(s) 598, 1066
Bse1I ACTGG 4 cut(s) 337, 535, 832, 1405
Bse3DI GCAATG 1 cut(s) 362
Bse8I GATNNNNATC 2 cut(s) 44, 774
BseAI TCCGGA 1 cut(s) 584
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 5 cut(s) 321, 370, 407, 645, 691
BseGI GGATG 3 cut(s) 209, 380, 432
BseJI GATNNNNATC 2 cut(s) 44, 774
BseLI CCNNNNNNNGG 6 cut(s) 371, 591, 731, 1033, 1091, 1374
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 1322
BseNI ACTGG 4 cut(s) 337, 535, 832, 1405
BseSI GKGCMC 2 cut(s) 274, 371
BseXI GCAGC 4 cut(s) 17, 107, 348, 775
Bsh1236I CGCG 2 cut(s) 409, 884
BshFI GGCC 3 cut(s) 208, 690, 1026
BshTI ACCGGT 1 cut(s) 1066
BsiHKAI GWGCWC 2 cut(s) 274, 485
BsiSI CCGG 6 cut(s) 213, 585, 599, 645, 1067, 1190
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 6 cut(s) 371, 591, 731, 1033, 1091, 1374
BsmAI GTCTC 3 cut(s) 203, 761, 1357
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 2 cut(s) 790, 1258
BsnI GGCC 3 cut(s) 208, 690, 1026
Bso31I GGTCTC 1 cut(s) 1357
Bsp119I TTCGAA 1 cut(s) 962
Bsp1286I GDGCHC 3 cut(s) 274, 371, 485
Bsp13I TCCGGA 1 cut(s) 584
Bsp143I GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
BspACI CCGC 6 cut(s) 123, 302, 407, 409, 839, 1167
BspANI GGCC 3 cut(s) 208, 690, 1026
BspCNI CTCAG 1 cut(s) 1321
BspEI TCCGGA 1 cut(s) 584
BspFNI CGCG 2 cut(s) 409, 884
BspLI GGNNCC 2 cut(s) 207, 925
BspMAI CTGCAG 2 cut(s) 13, 793
BspPI GGATC 3 cut(s) 595, 764, 1134
BspT104I TTCGAA 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 1357
BsrBI CCGCTC 1 cut(s) 839
BsrDI GCAATG 1 cut(s) 362
BsrFI RCCGGY 2 cut(s) 598, 1066
BsrI ACTGG 4 cut(s) 337, 535, 832, 1405
BssAI RCCGGY 2 cut(s) 598, 1066
BssECI CCNNGG 5 cut(s) 321, 370, 407, 645, 691
BssMI GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
BssNI GRCGYC 2 cut(s) 196, 848
BssSI CACGAG 2 cut(s) 478, 758
BssT1I CCWWGG 1 cut(s) 691
Bst2BI CACGAG 2 cut(s) 478, 758
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 6 cut(s) 263, 322, 904, 1003, 1153, 1426
BstACI GRCGYC 2 cut(s) 196, 848
BstBAI YACGTR 1 cut(s) 423
BstBI TTCGAA 1 cut(s) 962
BstC8I GCNNGC 5 cut(s) 13, 100, 341, 362, 793
BstDEI CTNAG 1 cut(s) 1308
BstDSI CCRYGG 2 cut(s) 321, 407
BstEII GGTNACC 2 cut(s) 215, 1079
BstF5I GGATG 3 cut(s) 209, 380, 432
BstFNI CGCG 2 cut(s) 409, 884
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 9 cut(s) 48, 499, 590, 772, 778, 858, 868, 1060, 1129
BstMAI GTCTC 3 cut(s) 203, 761, 1357
BstMBI GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 129, 361, 366, 375, 801
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 2 cut(s) 102, 976
BstPI GGTNACC 2 cut(s) 215, 1079
BstSCI CCNGG 2 cut(s) 370, 644
BstSFI CTRYAG 2 cut(s) 9, 789
BstSLI GKGCMC 2 cut(s) 274, 371
BstUI CGCG 2 cut(s) 409, 884
BstV1I GCAGC 4 cut(s) 17, 107, 348, 775
BstV2I GAAGAC 2 cut(s) 548, 1281
BsuRI GGCC 3 cut(s) 208, 690, 1026
BtgI CCRYGG 2 cut(s) 321, 407
BtgZI GCGATG 2 cut(s) 1401, 1419
BtsCI GGATG 3 cut(s) 209, 380, 432
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 4 cut(s) 109, 922, 1008, 1158
Cac8I GCNNGC 5 cut(s) 13, 100, 341, 362, 793
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 2 cut(s) 598, 1066
Cfr13I GGNCC 2 cut(s) 206, 532
Cfr42I CCGCGG 1 cut(s) 410
CseI GACGC 3 cut(s) 185, 361, 856
Csp6I GTAC 5 cut(s) 114, 134, 880, 886, 1398
CspAI ACCGGT 1 cut(s) 1066
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviAII CATG 7 cut(s) 99, 202, 397, 438, 529, 896, 973
CviQI GTAC 5 cut(s) 114, 134, 880, 886, 1398
DdeI CTNAG 1 cut(s) 1308
DpnI GATC 9 cut(s) 47, 498, 589, 771, 777, 857, 867, 1059, 1128
DpnII GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
EaeI YGGCCR 1 cut(s) 1024
EciI GGCGGA 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 1357
Eco47I GGWCC 1 cut(s) 532
Eco91I GGTNACC 2 cut(s) 215, 1079
EcoO65I GGTNACC 2 cut(s) 215, 1079
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 691
ErhI CCWWGG 1 cut(s) 691
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 7 cut(s) 102, 205, 400, 441, 532, 899, 976
FaqI GGGAC 1 cut(s) 263
FatI CATG 7 cut(s) 98, 201, 396, 437, 528, 895, 972
FauI CCCGC 2 cut(s) 116, 414
FauNDI CATATG 2 cut(s) 36, 347
FbaI TGATCA 4 cut(s) 45, 496, 775, 855
Fnu4HI GCNGC 5 cut(s) 6, 9, 96, 337, 789
FokI GGATG 3 cut(s) 196, 387, 419
Fsp4HI GCNGC 5 cut(s) 6, 9, 96, 337, 789
FspBI CTAG 2 cut(s) 87, 1073
GlaI GCGC 1 cut(s) 447
GluI GCNGC 5 cut(s) 6, 9, 96, 337, 789
HaeIII GGCC 3 cut(s) 208, 690, 1026
HapII CCGG 6 cut(s) 213, 585, 599, 645, 1067, 1190
HgaI GACGC 3 cut(s) 185, 361, 856
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 2 cut(s) 196, 848
Hin1II CATG 7 cut(s) 102, 205, 400, 441, 532, 899, 976
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 4 cut(s) 581, 912, 944, 983
HpaII CCGG 6 cut(s) 213, 585, 599, 645, 1067, 1190
HphI GGTGA 6 cut(s) 227, 275, 506, 770, 842, 1091
Hpy166II GTNNAC 3 cut(s) 272, 526, 632
Hpy188I TCNGA 4 cut(s) 238, 661, 734, 1062
Hpy188III TCNNGA 6 cut(s) 87, 486, 585, 773, 980, 987
Hpy8I GTNNAC 3 cut(s) 272, 526, 632
Hpy99I CGWCG 2 cut(s) 734, 850
HpyAV CCTTC 6 cut(s) 827, 902, 1042, 1157, 1210, 1366
HpyCH4III ACNGT 6 cut(s) 263, 322, 904, 1003, 1153, 1426
HpyCH4IV ACGT 8 cut(s) 276, 422, 522, 575, 729, 797, 1121, 1278
HpyCH4V TGCA 8 cut(s) 11, 102, 272, 510, 791, 1156, 1268, 1374
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 361, 366, 375, 801
HpyF3I CTNAG 1 cut(s) 1308
HpySE526I ACGT 8 cut(s) 276, 422, 522, 575, 729, 797, 1121, 1278
Hsp92I GRCGYC 2 cut(s) 196, 848
Hsp92II CATG 7 cut(s) 102, 205, 400, 441, 532, 899, 976
HspAI GCGC 1 cut(s) 446
Kpn2I TCCGGA 1 cut(s) 584
Ksp22I TGATCA 4 cut(s) 45, 496, 775, 855
KspI CCGCGG 1 cut(s) 410
Kzo9I GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
LmnI GCTCC 3 cut(s) 727, 836, 1139
Lsp1109I GCAGC 4 cut(s) 17, 107, 348, 775
LweI GCATC 1 cut(s) 365
MaeI CTAG 2 cut(s) 87, 1073
MaeII ACGT 8 cut(s) 276, 422, 522, 575, 729, 797, 1121, 1278
MaeIII GTNAC 8 cut(s) 179, 215, 241, 512, 823, 898, 997, 1079
MalI GATC 9 cut(s) 47, 498, 589, 771, 777, 857, 867, 1059, 1128
MbiI CCGCTC 1 cut(s) 839
MboI GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
MboII GAAGA 4 cut(s) 553, 1195, 1226, 1286
MfeI CAATTG 3 cut(s) 291, 465, 1028
MhlI GDGCHC 3 cut(s) 274, 371, 485
MlsI TGGCCA 1 cut(s) 1026
MluI ACGCGT 1 cut(s) 882
MluNI TGGCCA 1 cut(s) 1026
MlyI GAGTC 3 cut(s) 590, 938, 977
MmeI TCCRAC 4 cut(s) 558, 712, 901, 1104
MnlI CCTC 5 cut(s) 259, 427, 607, 728, 814
Mox20I TGGCCA 1 cut(s) 1026
MroI TCCGGA 1 cut(s) 584
MscI TGGCCA 1 cut(s) 1026
MseI TTAA 5 cut(s) 861, 1184, 1281, 1461, 1472
MslI CAYNNNNRTG 2 cut(s) 783, 932
Msp20I TGGCCA 1 cut(s) 1026
MspA1I CMGCKG 2 cut(s) 8, 409
MspI CCGG 6 cut(s) 213, 585, 599, 645, 1067, 1190
MspR9I CCNGG 2 cut(s) 372, 646
MunI CAATTG 3 cut(s) 291, 465, 1028
Mva1269I GAATGC 2 cut(s) 790, 1258
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 2 cut(s) 409, 884
MwoI GCNNNNNNNGC 5 cut(s) 129, 361, 366, 375, 801
NciI CCSGG 1 cut(s) 646
NdeI CATATG 2 cut(s) 36, 347
NdeII GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
NlaIII CATG 7 cut(s) 102, 205, 400, 441, 532, 899, 976
NlaIV GGNNCC 2 cut(s) 207, 925
NmuCI GTSAC 4 cut(s) 215, 898, 997, 1079
NspI RCATGY 2 cut(s) 102, 976
NspV TTCGAA 1 cut(s) 962
OliI CACNNNNGTG 1 cut(s) 932
PaeI GCATGC 1 cut(s) 102
PciI ACATGT 1 cut(s) 972
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 765
PctI GAATGC 2 cut(s) 790, 1258
PfeI GAWTC 1 cut(s) 912
PflMI CCANNNNNTGG 1 cut(s) 1033
PinAI ACCGGT 1 cut(s) 1066
PkrI GCNGC 5 cut(s) 7, 10, 97, 338, 790
PleI GAGTC 3 cut(s) 589, 938, 977
PpsI GAGTC 3 cut(s) 589, 938, 977
Ppu21I YACGTR 1 cut(s) 423
PscI ACATGT 1 cut(s) 972
Psp6I CCWGG 1 cut(s) 370
PspEI GGTNACC 2 cut(s) 215, 1079
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 207, 925
PspPI GGNCC 2 cut(s) 206, 532
PstI CTGCAG 2 cut(s) 13, 793
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 115, 135, 881, 887, 1399
RsaNI GTAC 5 cut(s) 114, 134, 880, 886, 1398
RseI CAYNNNNRTG 2 cut(s) 783, 932
SacII CCGCGG 1 cut(s) 410
SaqAI TTAA 5 cut(s) 861, 1184, 1281, 1461, 1472
SatI GCNGC 5 cut(s) 6, 9, 96, 337, 789
Sau3AI GATC 9 cut(s) 45, 496, 587, 769, 775, 855, 865, 1057, 1126
Sau96I GGNCC 2 cut(s) 206, 532
ScaI AGTACT 2 cut(s) 115, 1399
SchI GAGTC 3 cut(s) 590, 938, 977
ScrFI CCNGG 2 cut(s) 372, 646
SduI GDGCHC 3 cut(s) 274, 371, 485
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 2 cut(s) 9, 789
Sfr303I CCGCGG 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 962
SgrBI CCGCGG 1 cut(s) 410
SinI GGWCC 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 783, 932
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
SphI GCATGC 1 cut(s) 102
SsiI CCGC 6 cut(s) 123, 302, 407, 409, 839, 1167
SspMI CTAG 2 cut(s) 87, 1073
StyD4I CCNGG 2 cut(s) 370, 644
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 6 cut(s) 263, 322, 904, 1003, 1153, 1426
TaiI ACGT 8 cut(s) 279, 425, 525, 578, 732, 800, 1124, 1281
TaqI TCGA 7 cut(s) 402, 539, 768, 845, 962, 1313, 1332
TatI WGTACW 2 cut(s) 113, 1397
TfiI GAWTC 1 cut(s) 912
Tru1I TTAA 5 cut(s) 861, 1184, 1281, 1461, 1472
Tru9I TTAA 5 cut(s) 861, 1184, 1281, 1461, 1472
TscAI CASTG 4 cut(s) 109, 922, 1008, 1158
TseFI GTSAC 4 cut(s) 215, 898, 997, 1079
TseI GCWGC 5 cut(s) 5, 8, 95, 336, 788
Tsp45I GTSAC 4 cut(s) 215, 898, 997, 1079
TspDTI ATGAA 3 cut(s) 296, 1265, 1468
TspGWI ACGGA 2 cut(s) 828, 1107
TspRI CASTG 4 cut(s) 109, 922, 1008, 1158
Van91I CCANNNNNTGG 1 cut(s) 1033
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 3 cut(s) 160, 652, 1315
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 2 cut(s) 102, 976
XspI CTAG 2 cut(s) 87, 1073
ZrmI AGTACT 2 cut(s) 115, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.