Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 817 |
| AccII | CGCG | 1 cut(s) | 675 |
| AciI | CCGC | 7 cut(s) | 474, 480, 615, 673, 675, 720, 1245 |
| AclWI | GGATC | 4 cut(s) | 118, 404, 653, 1079 |
| AcuI | CTGAAG | 1 cut(s) | 861 |
| AcvI | CACGTG | 1 cut(s) | 209 |
| AcyI | GRCGYC | 1 cut(s) | 8 |
| AfaI | GTAC | 4 cut(s) | 281, 304, 1004, 1339 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 98, 702, 703, 1244 |
| AgsI | TTSAA | 3 cut(s) | 501, 911, 1033 |
| AjnI | CCWGG | 2 cut(s) | 561, 1132 |
| AluBI | AGCT | 7 cut(s) | 416, 439, 504, 680, 907, 1177, 1347 |
| AluI | AGCT | 7 cut(s) | 416, 439, 504, 680, 907, 1177, 1347 |
| Alw21I | GWGCWC | 1 cut(s) | 441 |
| Alw26I | GTCTC | 4 cut(s) | 543, 921, 1208, 1321 |
| AlwI | GGATC | 4 cut(s) | 118, 404, 653, 1079 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 839 |
| AoxI | GGCC | 3 cut(s) | 670, 957, 1135 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 728 |
| AspLEI | GCGC | 1 cut(s) | 881 |
| AspS9I | GGNCC | 2 cut(s) | 836, 1136 |
| AsuHPI | GGTGA | 5 cut(s) | 86, 542, 841, 884, 1087 |
| AvaII | GGWCC | 1 cut(s) | 836 |
| BaeGI | GKGCMC | 1 cut(s) | 1273 |
| BanII | GRGCYC | 1 cut(s) | 441 |
| BbrPI | CACGTG | 1 cut(s) | 209 |
| Bbv12I | GWGCWC | 1 cut(s) | 441 |
| BccI | CCATC | 3 cut(s) | 135, 148, 1198 |
| BceAI | ACGGC | 1 cut(s) | 944 |
| BciT130I | CCWGG | 2 cut(s) | 563, 1134 |
| BclI | TGATCA | 2 cut(s) | 253, 975 |
| BcoDI | GTCTC | 4 cut(s) | 543, 921, 1208, 1321 |
| BfaI | CTAG | 5 cut(s) | 284, 668, 1301, 1344, 1348 |
| BfmI | CTRYAG | 1 cut(s) | 265 |
| BfoI | RGCGCY | 1 cut(s) | 882 |
| BisI | GCNGC | 2 cut(s) | 480, 673 |
| BlpI | GCTNAGC | 2 cut(s) | 470, 1225 |
| BlsI | GCNGC | 2 cut(s) | 481, 674 |
| Bme1390I | CCNGG | 2 cut(s) | 563, 1134 |
| Bme18I | GGWCC | 1 cut(s) | 836 |
| BmgT120I | GGNCC | 2 cut(s) | 836, 1136 |
| BmiI | GGNNCC | 3 cut(s) | 478, 747, 1138 |
| BmrFI | CCNGG | 2 cut(s) | 563, 1134 |
| BmsI | GCATC | 3 cut(s) | 225, 934, 940 |
| BpmI | CTGGAG | 1 cut(s) | 1091 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 470, 1225 |
| BpuEI | CTTGAG | 2 cut(s) | 781, 923 |
| Bsa29I | ATCGAT | 1 cut(s) | 1063 |
| BsaAI | YACGTR | 1 cut(s) | 209 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1065 |
| BsaHI | GRCGYC | 1 cut(s) | 8 |
| BsaI | GGTCTC | 1 cut(s) | 543 |
| BsaJI | CCNNGG | 5 cut(s) | 97, 271, 561, 673, 954 |
| BsaWI | WCCGGW | 2 cut(s) | 338, 1009 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 98, 702, 703, 1244 |
| Bse1I | ACTGG | 3 cut(s) | 492, 553, 1096 |
| Bse3DI | GCAATG | 2 cut(s) | 30, 196 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1065 |
| BseBI | CCWGG | 2 cut(s) | 563, 1134 |
| BseCI | ATCGAT | 1 cut(s) | 1063 |
| BseDI | CCNNGG | 5 cut(s) | 97, 271, 561, 673, 954 |
| BseGI | GGATG | 3 cut(s) | 587, 1209, 1213 |
| BseJI | GATNNNNATC | 1 cut(s) | 1065 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 98, 702, 703, 1244 |
| BseMI | GCAATG | 2 cut(s) | 30, 196 |
| BseMII | CTCAG | 2 cut(s) | 899, 999 |
| BseNI | ACTGG | 3 cut(s) | 492, 553, 1096 |
| BseRI | GAGGAG | 3 cut(s) | 390, 645, 784 |
| BseSI | GKGCMC | 1 cut(s) | 1273 |
| BsgI | GTGCAG | 1 cut(s) | 1185 |
| Bsh1236I | CGCG | 1 cut(s) | 675 |
| BshFI | GGCC | 3 cut(s) | 672, 959, 1137 |
| BshVI | ATCGAT | 1 cut(s) | 1063 |
| BsiHKAI | GWGCWC | 1 cut(s) | 441 |
| BsiSI | CCGG | 2 cut(s) | 339, 1010 |
| BslFI | GGGAC | 1 cut(s) | 1003 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 98, 702, 703, 1244 |
| BsmAI | GTCTC | 4 cut(s) | 543, 921, 1208, 1321 |
| BsmFI | GGGAC | 1 cut(s) | 1003 |
| BsmI | GAATGC | 1 cut(s) | 1229 |
| BsnI | GGCC | 3 cut(s) | 672, 959, 1137 |
| Bso31I | GGTCTC | 1 cut(s) | 543 |
| Bsp1286I | GDGCHC | 2 cut(s) | 441, 1273 |
| Bsp1407I | TGTACA | 1 cut(s) | 1337 |
| Bsp143I | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 470, 1225 |
| Bsp19I | CCATGG | 1 cut(s) | 97 |
| BspACI | CCGC | 7 cut(s) | 474, 480, 615, 673, 675, 720, 1245 |
| BspANI | GGCC | 3 cut(s) | 672, 959, 1137 |
| BspCNI | CTCAG | 2 cut(s) | 898, 998 |
| BspDI | ATCGAT | 1 cut(s) | 1063 |
| BspFNI | CGCG | 1 cut(s) | 675 |
| BspLI | GGNNCC | 3 cut(s) | 478, 747, 1138 |
| BspPI | GGATC | 4 cut(s) | 118, 404, 653, 1079 |
| BspTNI | GGTCTC | 1 cut(s) | 543 |
| BsrDI | GCAATG | 2 cut(s) | 30, 196 |
| BsrGI | TGTACA | 1 cut(s) | 1337 |
| BsrI | ACTGG | 3 cut(s) | 492, 553, 1096 |
| BssECI | CCNNGG | 5 cut(s) | 97, 271, 561, 673, 954 |
| BssMI | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| BssNI | GRCGYC | 1 cut(s) | 8 |
| BssT1I | CCWWGG | 3 cut(s) | 97, 271, 954 |
| Bst2UI | CCWGG | 2 cut(s) | 563, 1134 |
| Bst4CI | ACNGT | 4 cut(s) | 224, 571, 1219, 1315 |
| Bst6I | CTCTTC | 1 cut(s) | 410 |
| BstACI | GRCGYC | 1 cut(s) | 8 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 372 |
| BstAUI | TGTACA | 1 cut(s) | 1337 |
| BstBAI | YACGTR | 1 cut(s) | 209 |
| BstC8I | GCNNGC | 1 cut(s) | 368 |
| BstDEI | CTNAG | 5 cut(s) | 61, 470, 885, 985, 1225 |
| BstDSI | CCRYGG | 2 cut(s) | 97, 673 |
| BstEII | GGTNACC | 1 cut(s) | 847 |
| BstF5I | GGATG | 3 cut(s) | 587, 1209, 1213 |
| BstFNI | CGCG | 1 cut(s) | 675 |
| BstH2I | RGCGCY | 1 cut(s) | 882 |
| BstHHI | GCGC | 1 cut(s) | 881 |
| BstKTI | GATC | 9 cut(s) | 126, 214, 256, 349, 412, 648, 978, 1063, 1087 |
| BstMAI | GTCTC | 4 cut(s) | 543, 921, 1208, 1321 |
| BstMBI | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 372 |
| BstNI | CCWGG | 2 cut(s) | 563, 1134 |
| BstNSI | RCATGY | 1 cut(s) | 370 |
| BstPI | GGTNACC | 1 cut(s) | 847 |
| BstSCI | CCNGG | 2 cut(s) | 561, 1132 |
| BstSFI | CTRYAG | 1 cut(s) | 265 |
| BstSLI | GKGCMC | 1 cut(s) | 1273 |
| BstUI | CGCG | 1 cut(s) | 675 |
| BstX2I | RGATCY | 1 cut(s) | 1084 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1160 |
| BstYI | RGATCY | 1 cut(s) | 1084 |
| Bsu15I | ATCGAT | 1 cut(s) | 1063 |
| BsuRI | GGCC | 3 cut(s) | 672, 959, 1137 |
| BsuTUI | ATCGAT | 1 cut(s) | 1063 |
| BtgI | CCRYGG | 2 cut(s) | 97, 673 |
| BtgZI | GCGATG | 1 cut(s) | 117 |
| BtsCI | GGATG | 3 cut(s) | 587, 1209, 1213 |
| BtsIMutI | CAGTG | 3 cut(s) | 546, 567, 646 |
| Cac8I | GCNNGC | 1 cut(s) | 368 |
| CaiI | CAGNNNCTG | 1 cut(s) | 839 |
| CfoI | GCGC | 1 cut(s) | 881 |
| Cfr13I | GGNCC | 2 cut(s) | 836, 1136 |
| Cfr42I | CCGCGG | 1 cut(s) | 676 |
| ClaI | ATCGAT | 1 cut(s) | 1063 |
| CseI | GACGC | 1 cut(s) | 16 |
| Csp6I | GTAC | 4 cut(s) | 280, 303, 1003, 1338 |
| CviAII | CATG | 5 cut(s) | 98, 121, 367, 372, 973 |
| CviQI | GTAC | 4 cut(s) | 280, 303, 1003, 1338 |
| DdeI | CTNAG | 5 cut(s) | 61, 470, 885, 985, 1225 |
| DpnI | GATC | 9 cut(s) | 125, 213, 255, 348, 411, 647, 977, 1062, 1086 |
| DpnII | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| Eam1104I | CTCTTC | 1 cut(s) | 410 |
| EarI | CTCTTC | 1 cut(s) | 410 |
| Ecl136II | GAGCTC | 1 cut(s) | 439 |
| Eco130I | CCWWGG | 3 cut(s) | 97, 271, 954 |
| Eco24I | GRGCYC | 1 cut(s) | 441 |
| Eco31I | GGTCTC | 1 cut(s) | 543 |
| Eco32I | GATATC | 1 cut(s) | 1067 |
| Eco47I | GGWCC | 1 cut(s) | 836 |
| Eco53kI | GAGCTC | 1 cut(s) | 439 |
| Eco57I | CTGAAG | 1 cut(s) | 861 |
| Eco72I | CACGTG | 1 cut(s) | 209 |
| Eco91I | GGTNACC | 1 cut(s) | 847 |
| EcoICRI | GAGCTC | 1 cut(s) | 439 |
| EcoO109I | RGGNCCY | 1 cut(s) | 836 |
| EcoO65I | GGTNACC | 1 cut(s) | 847 |
| EcoRII | CCWGG | 2 cut(s) | 561, 1132 |
| EcoRV | GATATC | 1 cut(s) | 1067 |
| EcoT14I | CCWWGG | 3 cut(s) | 97, 271, 954 |
| EcoT22I | ATGCAT | 1 cut(s) | 368 |
| EcoT38I | GRGCYC | 1 cut(s) | 441 |
| ErhI | CCWWGG | 3 cut(s) | 97, 271, 954 |
| FaeI | CATG | 5 cut(s) | 101, 124, 370, 375, 976 |
| FaqI | GGGAC | 1 cut(s) | 1003 |
| FatI | CATG | 5 cut(s) | 97, 120, 366, 371, 972 |
| FauI | CCCGC | 1 cut(s) | 713 |
| FauNDI | CATATG | 1 cut(s) | 526 |
| FbaI | TGATCA | 2 cut(s) | 253, 975 |
| FblI | GTMKAC | 1 cut(s) | 817 |
| Fnu4HI | GCNGC | 2 cut(s) | 480, 673 |
| FokI | GGATG | 3 cut(s) | 574, 1216, 1220 |
| FriOI | GRGCYC | 1 cut(s) | 441 |
| Fsp4HI | GCNGC | 2 cut(s) | 480, 673 |
| FspBI | CTAG | 5 cut(s) | 284, 668, 1301, 1344, 1348 |
| GlaI | GCGC | 1 cut(s) | 880 |
| GluI | GCNGC | 2 cut(s) | 480, 673 |
| GsuI | CTGGAG | 1 cut(s) | 1091 |
| HaeII | RGCGCY | 1 cut(s) | 882 |
| HaeIII | GGCC | 3 cut(s) | 672, 959, 1137 |
| HapII | CCGG | 2 cut(s) | 339, 1010 |
| HgaI | GACGC | 1 cut(s) | 16 |
| HhaI | GCGC | 1 cut(s) | 881 |
| Hin1I | GRCGYC | 1 cut(s) | 8 |
| Hin1II | CATG | 5 cut(s) | 101, 124, 370, 375, 976 |
| Hin6I | GCGC | 1 cut(s) | 879 |
| HinP1I | GCGC | 1 cut(s) | 879 |
| HincII | GTYRAC | 3 cut(s) | 168, 334, 818 |
| HindII | GTYRAC | 3 cut(s) | 168, 334, 818 |
| HinfI | GANTC | 5 cut(s) | 245, 581, 650, 983, 1254 |
| HpaI | GTTAAC | 1 cut(s) | 168 |
| HpaII | CCGG | 2 cut(s) | 339, 1010 |
| HphI | GGTGA | 5 cut(s) | 86, 542, 841, 884, 1087 |
| Hpy166II | GTNNAC | 6 cut(s) | 168, 206, 289, 305, 334, 818 |
| Hpy188I | TCNGA | 3 cut(s) | 238, 888, 1089 |
| Hpy188III | TCNNGA | 6 cut(s) | 131, 654, 839, 1070, 1098, 1111 |
| Hpy8I | GTNNAC | 6 cut(s) | 168, 206, 289, 305, 334, 818 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 166, 204 |
| HpyAV | CCTTC | 1 cut(s) | 1330 |
| HpyCH4III | ACNGT | 4 cut(s) | 224, 571, 1219, 1315 |
| HpyCH4IV | ACGT | 3 cut(s) | 202, 208, 1001 |
| HpyCH4V | TGCA | 5 cut(s) | 35, 366, 375, 947, 1166 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 372 |
| HpyF3I | CTNAG | 5 cut(s) | 61, 470, 885, 985, 1225 |
| HpySE526I | ACGT | 3 cut(s) | 202, 208, 1001 |
| Hsp92I | GRCGYC | 1 cut(s) | 8 |
| Hsp92II | CATG | 5 cut(s) | 101, 124, 370, 375, 976 |
| HspAI | GCGC | 1 cut(s) | 879 |
| Ksp22I | TGATCA | 2 cut(s) | 253, 975 |
| KspAI | GTTAAC | 1 cut(s) | 168 |
| KspI | CCGCGG | 1 cut(s) | 676 |
| Kzo9I | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| LmnI | GCTCC | 2 cut(s) | 476, 677 |
| LweI | GCATC | 3 cut(s) | 225, 934, 940 |
| MaeI | CTAG | 5 cut(s) | 284, 668, 1301, 1344, 1348 |
| MaeII | ACGT | 3 cut(s) | 202, 208, 1001 |
| MaeIII | GTNAC | 7 cut(s) | 131, 509, 530, 571, 847, 968, 1144 |
| MalI | GATC | 9 cut(s) | 125, 213, 255, 348, 411, 647, 977, 1062, 1086 |
| MboI | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| MboII | GAAGA | 5 cut(s) | 135, 251, 397, 411, 1121 |
| MfeI | CAATTG | 1 cut(s) | 1264 |
| MflI | RGATCY | 1 cut(s) | 1084 |
| MhlI | GDGCHC | 2 cut(s) | 441, 1273 |
| MluCI | AATT | 7 cut(s) | 108, 157, 327, 496, 657, 1041, 1264 |
| MlyI | GAGTC | 2 cut(s) | 659, 977 |
| MmeI | TCCRAC | 5 cut(s) | 477, 643, 904, 1112, 1231 |
| MnlI | CCTC | 9 cut(s) | 102, 125, 408, 411, 493, 623, 805, 1042, 1066 |
| Mph1103I | ATGCAT | 1 cut(s) | 368 |
| MroXI | GAANNNNTTC | 1 cut(s) | 728 |
| MseI | TTAA | 6 cut(s) | 167, 822, 963, 998, 1056, 1158 |
| MslI | CAYNNNNRTG | 3 cut(s) | 102, 624, 1158 |
| MspA1I | CMGCKG | 1 cut(s) | 675 |
| MspI | CCGG | 2 cut(s) | 339, 1010 |
| MspR9I | CCNGG | 2 cut(s) | 563, 1134 |
| MunI | CAATTG | 1 cut(s) | 1264 |
| Mva1269I | GAATGC | 1 cut(s) | 1229 |
| MvaI | CCWGG | 2 cut(s) | 563, 1134 |
| MvnI | CGCG | 1 cut(s) | 675 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 372 |
| NcoI | CCATGG | 1 cut(s) | 97 |
| NdeI | CATATG | 1 cut(s) | 526 |
| NdeII | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| NlaIII | CATG | 5 cut(s) | 101, 124, 370, 375, 976 |
| NlaIV | GGNNCC | 3 cut(s) | 478, 747, 1138 |
| NmuCI | GTSAC | 5 cut(s) | 131, 509, 530, 847, 1144 |
| NsiI | ATGCAT | 1 cut(s) | 368 |
| NspI | RCATGY | 1 cut(s) | 370 |
| PaeI | GCATGC | 1 cut(s) | 370 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 220 |
| PctI | GAATGC | 1 cut(s) | 1229 |
| PdmI | GAANNNNTTC | 1 cut(s) | 728 |
| PfeI | GAWTC | 3 cut(s) | 245, 581, 1254 |
| PkrI | GCNGC | 2 cut(s) | 481, 674 |
| PleI | GAGTC | 2 cut(s) | 658, 977 |
| PmaCI | CACGTG | 1 cut(s) | 209 |
| PmlI | CACGTG | 1 cut(s) | 209 |
| PpsI | GAGTC | 2 cut(s) | 658, 977 |
| Ppu21I | YACGTR | 1 cut(s) | 209 |
| PpuMI | RGGWCCY | 1 cut(s) | 836 |
| Psp124BI | GAGCTC | 1 cut(s) | 441 |
| Psp5II | RGGWCCY | 1 cut(s) | 836 |
| Psp6I | CCWGG | 2 cut(s) | 561, 1132 |
| PspCI | CACGTG | 1 cut(s) | 209 |
| PspEI | GGTNACC | 1 cut(s) | 847 |
| PspGI | CCWGG | 2 cut(s) | 561, 1132 |
| PspN4I | GGNNCC | 3 cut(s) | 478, 747, 1138 |
| PspPI | GGNCC | 2 cut(s) | 836, 1136 |
| PspPPI | RGGWCCY | 1 cut(s) | 836 |
| PstNI | CAGNNNCTG | 1 cut(s) | 839 |
| PsuI | RGATCY | 1 cut(s) | 1084 |
| RsaI | GTAC | 4 cut(s) | 281, 304, 1004, 1339 |
| RsaNI | GTAC | 4 cut(s) | 280, 303, 1003, 1338 |
| RseI | CAYNNNNRTG | 3 cut(s) | 102, 624, 1158 |
| SacI | GAGCTC | 1 cut(s) | 441 |
| SacII | CCGCGG | 1 cut(s) | 676 |
| SalI | GTCGAC | 1 cut(s) | 816 |
| SaqAI | TTAA | 6 cut(s) | 167, 822, 963, 998, 1056, 1158 |
| SatI | GCNGC | 2 cut(s) | 480, 673 |
| Sau3AI | GATC | 9 cut(s) | 123, 211, 253, 346, 409, 645, 975, 1060, 1084 |
| Sau96I | GGNCC | 2 cut(s) | 836, 1136 |
| SchI | GAGTC | 2 cut(s) | 659, 977 |
| ScrFI | CCNGG | 2 cut(s) | 563, 1134 |
| SduI | GDGCHC | 2 cut(s) | 441, 1273 |
| SfaNI | GCATC | 3 cut(s) | 225, 934, 940 |
| SfcI | CTRYAG | 1 cut(s) | 265 |
| Sfr303I | CCGCGG | 1 cut(s) | 676 |
| SgrBI | CCGCGG | 1 cut(s) | 676 |
| SinI | GGWCC | 1 cut(s) | 836 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 102, 624, 1158 |
| SmlI | CTYRAG | 2 cut(s) | 796, 902 |
| SmoI | CTYRAG | 2 cut(s) | 796, 902 |
| SphI | GCATGC | 1 cut(s) | 370 |
| Sse9I | AATT | 7 cut(s) | 108, 157, 327, 496, 657, 1041, 1264 |
| SsiI | CCGC | 7 cut(s) | 474, 480, 615, 673, 675, 720, 1245 |
| SspI | AATATT | 2 cut(s) | 521, 728 |
| SspMI | CTAG | 5 cut(s) | 284, 668, 1301, 1344, 1348 |
| SstI | GAGCTC | 1 cut(s) | 441 |
| StyD4I | CCNGG | 2 cut(s) | 561, 1132 |
| StyI | CCWWGG | 3 cut(s) | 97, 271, 954 |
| TaaI | ACNGT | 4 cut(s) | 224, 571, 1219, 1315 |
| TaiI | ACGT | 3 cut(s) | 205, 211, 1004 |
| TaqI | TCGA | 7 cut(s) | 41, 387, 408, 648, 817, 866, 1063 |
| TasI | AATT | 7 cut(s) | 108, 157, 327, 496, 657, 1041, 1264 |
| TatI | WGTACW | 1 cut(s) | 1337 |
| TauI | GCSGC | 2 cut(s) | 482, 675 |
| TfiI | GAWTC | 3 cut(s) | 245, 581, 1254 |
| Tru1I | TTAA | 6 cut(s) | 167, 822, 963, 998, 1056, 1158 |
| Tru9I | TTAA | 6 cut(s) | 167, 822, 963, 998, 1056, 1158 |
| TscAI | CASTG | 3 cut(s) | 553, 574, 646 |
| TseFI | GTSAC | 5 cut(s) | 131, 509, 530, 847, 1144 |
| Tsp45I | GTSAC | 5 cut(s) | 131, 509, 530, 847, 1144 |
| TspDTI | ATGAA | 6 cut(s) | 217, 258, 310, 411, 513, 819 |
| TspRI | CASTG | 3 cut(s) | 553, 574, 646 |
| VpaK11BI | GGWCC | 1 cut(s) | 836 |
| XceI | RCATGY | 1 cut(s) | 370 |
| XmiI | GTMKAC | 1 cut(s) | 817 |
| XmnI | GAANNNNTTC | 1 cut(s) | 728 |
| XspI | CTAG | 5 cut(s) | 284, 668, 1301, 1344, 1348 |
| Zsp2I | ATGCAT | 1 cut(s) | 368 |