MD12G1242700.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000230
Physical Location & Seq
Reverse (-)
31382197 .. 31382988
792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1242700.v1.1.491

Sequence Viewer

Length: 792 bp
ATGTTGTGGACGCAACGTGAGCCGTGTCCGAATTGCGACAAGCAGAAAAATCCCAAGTTTGATCCGAAAAAGTCCTCACCATATGCAACCCTTCCATGTACTGCACAAGAATGTAGTCATACTAATGACACTGGCTACACCTCTTGCTCAAAGGATGATCAAAAGGTCTGCAACTACAATTACACTTACATGGATGATTCAATAACGCAGGGGGTGATGTCAAAAGAGACAATCACATTCGGATCCAGCTCCGGGAAGCCAGTTTCGTTCAAAGATGTTGTGATAGGTTGTGGACACAACAATACTGGGAAAACCTTCGGCGAAAACGAAATGGGGATAGTAGGGGTTGGAGCTGGGAATTTATCGATTATTTCTCAACTTGCTCCTTACGTTGGAGGCAAGAAATTCTCACATTGTCTCGTGCCCTTTGATCCGGATCATCCCAATGATGCAAGCATCATGAGTTTCGGAAAGGGAAGTGAGGTTTCGGGTGAAGGTGTAGTATCGACTCCATTAATCACCAAACATGGCAAAAACCAATATTTTTTCACGGTTGAAGGAATCAGCGTCGGAGAACAGTTTGTGCCATTTAACTCATCAGGTTCGGTTTCAAAGGGGAACATGTATCTCGACTCGGGTACACAAGTGACAATGCTGCCACAAGATTTTTACGGCAGATTGGTAGAACAAGTAAAGAAGACGATGAATTCGTCAGTAAAACCAGTCGAAGTAAATGACGAGTCTGGAACTTTGCTGTGCTACAACACCACGGAGAATCTCAAAGCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

264

Amino Acids

28.49

Weight (kDa)

5.75

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 1 - 158 2.2e-35 Xylanase inhibitor N-terminal
TAXi_C PF14541 180 - 258 1.7e-12 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 433
AclWI GGATC 5 cut(s) 56, 237, 250, 425, 444
AcsI RAATTY 3 cut(s) 358, 404, 706
AfaI GTAC 2 cut(s) 100, 640
AfiI CCNNNNNNNGG 2 cut(s) 252, 392
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 4 cut(s) 201, 271, 557, 612
AluBI AGCT 2 cut(s) 249, 353
AluI AGCT 2 cut(s) 249, 353
Alw26I GTCTC 2 cut(s) 221, 422
AlwI GGATC 5 cut(s) 56, 237, 250, 425, 444
Ama87I CYCGRG 1 cut(s) 634
Aor13HI TCCGGA 1 cut(s) 433
ApeKI GCWGC 1 cut(s) 655
ApoI RAATTY 3 cut(s) 358, 404, 706
ArsI GACNNNNNNTTYG 2 cut(s) 691, 723
AseI ATTAAT 1 cut(s) 515
Asp700I GAANNNNTTC 1 cut(s) 314
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 4 cut(s) 69, 226, 503, 511
AvaI CYCGRG 1 cut(s) 634
BaeGI GKGCMC 1 cut(s) 426
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BamHI GGATCC 1 cut(s) 242
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 704
BbvI GCAGC 1 cut(s) 642
BceAI ACGGC 2 cut(s) 7, 688
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 221, 422
BisI GCNGC 1 cut(s) 656
BlsI GCNGC 1 cut(s) 657
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 634
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 439, 465
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 704
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 768
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 392
Bse1I ACTGG 4 cut(s) 136, 260, 310, 722
Bse8I GATNNNNATC 1 cut(s) 435
BseAI TCCGGA 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 768
BseGI GGATG 3 cut(s) 160, 199, 439
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 252, 392
BseNI ACTGG 4 cut(s) 136, 260, 310, 722
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 1 cut(s) 642
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 87
BshVI ATCGAT 1 cut(s) 365
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 2 cut(s) 252, 434
BslI CCNNNNNNNGG 2 cut(s) 252, 392
BsmAI GTCTC 2 cut(s) 221, 422
BsoBI CYCGRG 1 cut(s) 634
Bsp1286I GDGCHC 1 cut(s) 426
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 5 cut(s) 61, 157, 242, 430, 436
BspDI ATCGAT 1 cut(s) 365
BspEI TCCGGA 1 cut(s) 433
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 5 cut(s) 56, 237, 250, 425, 444
BsrI ACTGG 4 cut(s) 136, 260, 310, 722
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 5 cut(s) 61, 157, 242, 430, 436
BssSI CACGAG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 553, 579
BstC8I GCNNGC 1 cut(s) 454
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 3 cut(s) 160, 199, 439
BstKTI GATC 5 cut(s) 64, 160, 245, 433, 439
BstMAI GTCTC 2 cut(s) 221, 422
BstMBI GATC 5 cut(s) 61, 157, 242, 430, 436
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 642
BstV2I GAAGAC 1 cut(s) 704
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 365
BsuTUI ATCGAT 1 cut(s) 365
BtgI CCRYGG 1 cut(s) 768
BtsCI GGATG 3 cut(s) 160, 199, 439
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 454
CciI TCATGA 1 cut(s) 459
ClaI ATCGAT 1 cut(s) 365
CseI GACGC 2 cut(s) 19, 556
Csp6I GTAC 2 cut(s) 99, 639
CviAII CATG 6 cut(s) 96, 190, 460, 527, 622, 789
CviJI RGCY 5 cut(s) 22, 135, 249, 259, 353
CviKI_1 RGCY 5 cut(s) 22, 135, 249, 259, 353
CviQI GTAC 2 cut(s) 99, 639
DpnI GATC 5 cut(s) 63, 159, 244, 432, 438
DpnII GATC 5 cut(s) 61, 157, 242, 430, 436
Eco88I CYCGRG 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 706
FaeI CATG 6 cut(s) 99, 193, 463, 530, 625, 792
FaiI YATR 9 cut(s) 82, 84, 97, 120, 191, 461, 528, 623, 790
FatI CATG 6 cut(s) 95, 189, 459, 526, 621, 788
FauNDI CATATG 1 cut(s) 82
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 656
FokI GGATG 3 cut(s) 167, 206, 426
Fsp4HI GCNGC 1 cut(s) 656
GluI GCNGC 1 cut(s) 656
GsaI CCCAGC 1 cut(s) 357
HapII CCGG 2 cut(s) 252, 434
HgaI GACGC 2 cut(s) 19, 556
Hin1II CATG 6 cut(s) 99, 193, 463, 530, 625, 792
HinfI GANTC 6 cut(s) 197, 508, 561, 632, 740, 775
HpaII CCGG 2 cut(s) 252, 434
HphI GGTGA 4 cut(s) 69, 226, 503, 511
Hpy166II GTNNAC 3 cut(s) 9, 293, 641
Hpy188I TCNGA 5 cut(s) 30, 66, 242, 470, 572
Hpy188III TCNNGA 4 cut(s) 434, 460, 629, 744
Hpy8I GTNNAC 3 cut(s) 9, 293, 641
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 4 cut(s) 101, 325, 488, 551
HpyCH4III ACNGT 2 cut(s) 553, 579
HpyCH4IV ACGT 2 cut(s) 16, 390
HpyCH4V TGCA 4 cut(s) 86, 104, 171, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpySE526I ACGT 2 cut(s) 16, 390
Hsp92II CATG 6 cut(s) 99, 193, 463, 530, 625, 792
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 61, 157, 242, 430, 436
LmnI GCTCC 3 cut(s) 254, 350, 388
Lsp1109I GCAGC 1 cut(s) 642
LweI GCATC 2 cut(s) 439, 465
MaeII ACGT 2 cut(s) 16, 390
MaeIII GTNAC 1 cut(s) 646
MalI GATC 5 cut(s) 63, 159, 244, 432, 438
MboI GATC 5 cut(s) 61, 157, 242, 430, 436
MboII GAAGA 1 cut(s) 709
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 5 cut(s) 31, 178, 358, 404, 706
MlyI GAGTC 3 cut(s) 502, 626, 749
MmeI TCCRAC 3 cut(s) 328, 373, 550
MnlI CCTC 4 cut(s) 85, 151, 389, 475
MroI TCCGGA 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 2 cut(s) 515, 591
MslI CAYNNNNRTG 4 cut(s) 109, 123, 188, 444
MspI CCGG 2 cut(s) 252, 434
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 82
NdeII GATC 5 cut(s) 61, 157, 242, 430, 436
NlaIII CATG 6 cut(s) 99, 193, 463, 530, 625, 792
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 625
PagI TCATGA 1 cut(s) 459
PciI ACATGT 1 cut(s) 621
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 707
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 3 cut(s) 197, 561, 775
PfoI TCCNGGA 1 cut(s) 251
PkrI GCNGC 1 cut(s) 657
PleI GAGTC 3 cut(s) 502, 626, 748
PpsI GAGTC 3 cut(s) 502, 626, 748
PscI ACATGT 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 515
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 244
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 2 cut(s) 100, 640
RsaNI GTAC 2 cut(s) 99, 639
RseI CAYNNNNRTG 4 cut(s) 109, 123, 188, 444
SaqAI TTAA 2 cut(s) 515, 591
SatI GCNGC 1 cut(s) 656
Sau3AI GATC 5 cut(s) 61, 157, 242, 430, 436
SchI GAGTC 3 cut(s) 502, 626, 749
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 2 cut(s) 439, 465
SmiMI CAYNNNNRTG 4 cut(s) 109, 123, 188, 444
Sse9I AATT 5 cut(s) 31, 178, 358, 404, 706
SspI AATATT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 251
TaaI ACNGT 2 cut(s) 553, 579
TaiI ACGT 2 cut(s) 19, 393
TaqI TCGA 4 cut(s) 365, 506, 630, 726
TasI AATT 5 cut(s) 31, 178, 358, 404, 706
TatI WGTACW 1 cut(s) 98
TfiI GAWTC 3 cut(s) 197, 561, 775
Tru1I TTAA 2 cut(s) 515, 591
Tru9I TTAA 2 cut(s) 515, 591
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 1 cut(s) 655
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 719
TspGWI ACGGA 1 cut(s) 785
TspRI CASTG 1 cut(s) 136
VspI ATTAAT 1 cut(s) 515
XapI RAATTY 3 cut(s) 358, 404, 706
XceI RCATGY 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.