Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0000230

Physical Location & Seq

Genomic Coordinates Forward (+)

30429209 .. 30430570

Gene Structure 1362 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00407810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGGCGACGCCATATCATTCGCTTTCCACCATTGCAACTTCCATTGTTATTATCTCTCTCTTAGTTATCTCTCACGCAGAAGATTATGTCCCTCACCATGGCGATGTAATTAGCCTCAACATGATCCATCGTGACACACCATCTTCTCCCTTATACAATTCGTCGTTAACTAAATGGCAACGAGTAAGCAATGCCTTGCGACGTTCACGTGATCGCATCAACCGTTTCATCTCATCAGAAGATGAATCGCCTGATCATCTAATCTATAGCCAAGGAGAGTACCTGGTGAACATATCAATCGGTACACCACCAAGAACTTTCATTGGAATTGTTGACACCGGAAGCGATCTTATATGGACACAATGCATGCCATGCAGGGAATGTTTCGAGCAAAACCCTCCTCTCTTCGATCCAGCTTCTTCATCAACTTATAGAGAGCTCTCTTGTATGTCCAACGAATGTAATTTGCTAAGCGGAGCCGCCTCTTGTTCCAGTAATTCAAGCTCTTGTGACTATCAATATTCATATGGTGACGGGTCTCATACCACCGGAAATCTATCCCTGGACACTGTTACTTTGGATTCCACTGTTTCCACATACATTCCCAACACTATATTTGGCTGCGGACACCATAATGGTGGAGTGTTCAGTGGGATCGAGTCAGGAATTATTGGACTAGGCCGCGGAGCTGTGTCGTTTATCTCCCAAATAGGGGGTTATTTGGTTGGCGGGAAATATTTCTCTTATTGCTTGGTTCCTAATCCTGCAATGTCTTTCCTGTCTAATAACTCTAATCCAATCTCCTCAAGCAAGATGTATTTTGGTCGACTTAATACTTCATCAGGTCCTGAAGTGGTCACCACCCCTTTGCTTTCGACAGGTGATAGCGCCACTCAGACCTTTTATTACCTTGAGCTTCAAGGCATTAGTGTTGGAGCCAAAAATATCACACTTGATGCAGATGCCAAGGCCTTTAAGGGTAACATGATCATAGACTCAGGGACTACCTTAACGTACTTACCGGAGTTGCTTTATGACTGGTTCAAATCAGCAATTAGAGAGGCACTTAAAGATCGATATCTGGAGGTGAAGCAAGATCCGACTGGTTCTGGACTTTGTTTCATGACTAAATATGATATTCTTCCTGGCCCCAATGTCATTCTCCATTTTAATGGTGCAGATGTGGAGCTAAAATCCACCAACACTTTTATTAGGATGGATGATGAGACTGTTTGCTTAGCATTCTCCCCAAGCAGCGGTGTCGGAATCTATGGGAATTGGGCACAGATAAACTACTTGGTAGGATATGACCTAGATAACAAGACTGTCTCCTTCCAGCCAACTGATTGCATGACTAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04138 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04138 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

453

Amino Acids

49.4

Weight (kDa)

4.95

Isoelectric Point (pI)

39.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	93 - 147	6.2e-08	Eukaryotic aspartyl protease
TAXi_N	PF14543	94 - 256	1.8e-50	Xylanase inhibitor N-terminal
Asp	PF00026	160 - 446	1.5e-16	Eukaryotic aspartyl protease
TAXi_C	PF14541	302 - 446	1.6e-23	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000230)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica	MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica	Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis	pycom12g22170
rosa_chinensis	RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata	RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora	Rmu_sc0034928.1_g000007
rosa_roxburghii	Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa	Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha	Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana	Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	826
AccII	CGCG	1 cut(s)	684
AciI	CCGC	7 cut(s)	474, 480, 624, 682, 684, 729, 1257
AclWI	GGATC	4 cut(s)	118, 404, 662, 1091
AcuI	CTGAAG	1 cut(s)	870
AcvI	CACGTG	1 cut(s)	209
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	3 cut(s)	281, 304, 1016
AfiI	CCNNNNNNNGG	4 cut(s)	98, 711, 712, 1256
AgsI	TTSAA	3 cut(s)	501, 920, 1045
AjnI	CCWGG	3 cut(s)	282, 561, 1144
AluBI	AGCT	7 cut(s)	416, 439, 504, 689, 916, 1189, 1359
AluI	AGCT	7 cut(s)	416, 439, 504, 689, 916, 1189, 1359
Alw21I	GWGCWC	1 cut(s)	441
Alw26I	GTCTC	3 cut(s)	543, 1220, 1333
AlwI	GGATC	4 cut(s)	118, 404, 662, 1091
AlwNI	CAGNNNCTG	1 cut(s)	848
AoxI	GGCC	3 cut(s)	679, 969, 1147
ApeKI	GCWGC	2 cut(s)	621, 1254
Asp700I	GAANNNNTTC	1 cut(s)	737
AspLEI	GCGC	1 cut(s)	890
AspS9I	GGNCC	2 cut(s)	845, 1148
AsuHPI	GGTGA	6 cut(s)	86, 298, 542, 850, 893, 1099
AvaII	GGWCC	1 cut(s)	845
BaeGI	GKGCMC	1 cut(s)	1285
BanII	GRGCYC	1 cut(s)	441
BbrPI	CACGTG	1 cut(s)	209
Bbv12I	GWGCWC	1 cut(s)	441
BbvI	GCAGC	2 cut(s)	608, 1266
BccI	CCATC	3 cut(s)	135, 148, 1210
BcgI	CGANNNNNNTGC	2 cut(s)	1243, 1277
BciT130I	CCWGG	3 cut(s)	284, 563, 1146
BclI	TGATCA	2 cut(s)	253, 987
BcoDI	GTCTC	3 cut(s)	543, 1220, 1333
BfaI	CTAG	4 cut(s)	677, 1313, 1356, 1360
BfmI	CTRYAG	1 cut(s)	265
BfoI	RGCGCY	1 cut(s)	891
BisI	GCNGC	4 cut(s)	480, 622, 682, 1255
BlpI	GCTNAGC	2 cut(s)	470, 1237
BlsI	GCNGC	4 cut(s)	481, 623, 683, 1256
Bme1390I	CCNGG	3 cut(s)	284, 563, 1146
Bme18I	GGWCC	1 cut(s)	845
BmgT120I	GGNCC	2 cut(s)	845, 1148
BmiI	GGNNCC	4 cut(s)	478, 756, 937, 1150
BmrFI	CCNGG	3 cut(s)	284, 563, 1146
BmsI	GCATC	3 cut(s)	225, 946, 952
BpmI	CTGGAG	1 cut(s)	1103
Bpu1102I	GCTNAGC	2 cut(s)	470, 1237
BpuEI	CTTGAG	2 cut(s)	790, 932
Bsa29I	ATCGAT	1 cut(s)	1075
BsaAI	YACGTR	1 cut(s)	209
BsaBI	GATNNNNATC	1 cut(s)	1077
BsaHI	GRCGYC	1 cut(s)	8
BsaI	GGTCTC	1 cut(s)	543
BsaJI	CCNNGG	5 cut(s)	97, 271, 561, 682, 966
BsaWI	WCCGGW	3 cut(s)	338, 548, 1021
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 711, 712, 1256
Bse1I	ACTGG	3 cut(s)	492, 1043, 1108
Bse3DI	GCAATG	3 cut(s)	30, 196, 774
Bse8I	GATNNNNATC	1 cut(s)	1077
BseBI	CCWGG	3 cut(s)	284, 563, 1146
BseCI	ATCGAT	1 cut(s)	1075
BseDI	CCNNGG	5 cut(s)	97, 271, 561, 682, 966
BseGI	GGATG	2 cut(s)	1221, 1225
BseJI	GATNNNNATC	1 cut(s)	1077
BseLI	CCNNNNNNNGG	4 cut(s)	98, 711, 712, 1256
BseMI	GCAATG	3 cut(s)	30, 196, 774
BseMII	CTCAG	2 cut(s)	908, 1011
BseNI	ACTGG	3 cut(s)	492, 1043, 1108
BseRI	GAGGAG	2 cut(s)	390, 793
BseSI	GKGCMC	1 cut(s)	1285
BseXI	GCAGC	2 cut(s)	608, 1266
BsgI	GTGCAG	1 cut(s)	1197
Bsh1236I	CGCG	1 cut(s)	684
BshFI	GGCC	3 cut(s)	681, 971, 1149
BshVI	ATCGAT	1 cut(s)	1075
BsiHKAI	GWGCWC	1 cut(s)	441
BsiSI	CCGG	3 cut(s)	339, 549, 1022
BslFI	GGGAC	2 cut(s)	74, 1015
BslI	CCNNNNNNNGG	4 cut(s)	98, 711, 712, 1256
BsmAI	GTCTC	3 cut(s)	543, 1220, 1333
BsmFI	GGGAC	2 cut(s)	74, 1015
BsmI	GAATGC	1 cut(s)	1241
BsnI	GGCC	3 cut(s)	681, 971, 1149
Bso31I	GGTCTC	1 cut(s)	543
Bsp1286I	GDGCHC	2 cut(s)	441, 1285
Bsp143I	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
Bsp1720I	GCTNAGC	2 cut(s)	470, 1237
Bsp19I	CCATGG	1 cut(s)	97
BspACI	CCGC	7 cut(s)	474, 480, 624, 682, 684, 729, 1257
BspANI	GGCC	3 cut(s)	681, 971, 1149
BspCNI	CTCAG	2 cut(s)	907, 1010
BspDI	ATCGAT	1 cut(s)	1075
BspFNI	CGCG	1 cut(s)	684
BspHI	TCATGA	1 cut(s)	1122
BspLI	GGNNCC	4 cut(s)	478, 756, 937, 1150
BspPI	GGATC	4 cut(s)	118, 404, 662, 1091
BspTNI	GGTCTC	1 cut(s)	543
BsrDI	GCAATG	3 cut(s)	30, 196, 774
BsrI	ACTGG	3 cut(s)	492, 1043, 1108
BssECI	CCNNGG	5 cut(s)	97, 271, 561, 682, 966
BssMI	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
BssNI	GRCGYC	1 cut(s)	8
BssT1I	CCWWGG	3 cut(s)	97, 271, 966
Bst2UI	CCWGG	3 cut(s)	284, 563, 1146
Bst4CI	ACNGT	5 cut(s)	224, 571, 589, 1231, 1327
Bst6I	CTCTTC	1 cut(s)	410
BstACI	GRCGYC	1 cut(s)	8
BstAPI	GCANNNNNTGC	1 cut(s)	372
BstBAI	YACGTR	1 cut(s)	209
BstC8I	GCNNGC	1 cut(s)	368
BstDEI	CTNAG	5 cut(s)	61, 470, 894, 997, 1237
BstDSI	CCRYGG	2 cut(s)	97, 682
BstEII	GGTNACC	1 cut(s)	856
BstF5I	GGATG	2 cut(s)	1221, 1225
BstFNI	CGCG	1 cut(s)	684
BstH2I	RGCGCY	1 cut(s)	891
BstHHI	GCGC	1 cut(s)	890
BstKTI	GATC	9 cut(s)	126, 214, 256, 349, 412, 657, 990, 1075, 1099
BstMAI	GTCTC	3 cut(s)	543, 1220, 1333
BstMBI	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
BstMWI	GCNNNNNNNGC	2 cut(s)	372, 1356
BstNI	CCWGG	3 cut(s)	284, 563, 1146
BstNSI	RCATGY	1 cut(s)	370
BstPI	GGTNACC	1 cut(s)	856
BstSCI	CCNGG	3 cut(s)	282, 561, 1144
BstSFI	CTRYAG	1 cut(s)	265
BstSLI	GKGCMC	1 cut(s)	1285
BstUI	CGCG	1 cut(s)	684
BstV1I	GCAGC	2 cut(s)	608, 1266
BstX2I	RGATCY	1 cut(s)	1096
BstXI	CCANNNNNNTGG	2 cut(s)	638, 1172
BstYI	RGATCY	1 cut(s)	1096
Bsu15I	ATCGAT	1 cut(s)	1075
BsuRI	GGCC	3 cut(s)	681, 971, 1149
BsuTUI	ATCGAT	1 cut(s)	1075
BtgI	CCRYGG	2 cut(s)	97, 682
BtgZI	GCGATG	1 cut(s)	117
BtsCI	GGATG	2 cut(s)	1221, 1225
BtsIMutI	CAGTG	3 cut(s)	567, 585, 655
Cac8I	GCNNGC	1 cut(s)	368
CaiI	CAGNNNCTG	1 cut(s)	848
CciI	TCATGA	1 cut(s)	1122
CfoI	GCGC	1 cut(s)	890
Cfr13I	GGNCC	2 cut(s)	845, 1148
Cfr42I	CCGCGG	1 cut(s)	685
ClaI	ATCGAT	1 cut(s)	1075
CseI	GACGC	1 cut(s)	16
CsiI	ACCWGGT	1 cut(s)	282
Csp6I	GTAC	3 cut(s)	280, 303, 1015
CviAII	CATG	7 cut(s)	98, 121, 367, 372, 985, 1123, 1351
CviQI	GTAC	3 cut(s)	280, 303, 1015
DdeI	CTNAG	5 cut(s)	61, 470, 894, 997, 1237
DpnI	GATC	9 cut(s)	125, 213, 255, 348, 411, 656, 989, 1074, 1098
DpnII	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
Eam1104I	CTCTTC	1 cut(s)	410
EarI	CTCTTC	1 cut(s)	410
Ecl136II	GAGCTC	1 cut(s)	439
Eco130I	CCWWGG	3 cut(s)	97, 271, 966
Eco147I	AGGCCT	1 cut(s)	971
Eco24I	GRGCYC	1 cut(s)	441
Eco31I	GGTCTC	1 cut(s)	543
Eco32I	GATATC	1 cut(s)	1079
Eco47I	GGWCC	1 cut(s)	845
Eco53kI	GAGCTC	1 cut(s)	439
Eco57I	CTGAAG	1 cut(s)	870
Eco72I	CACGTG	1 cut(s)	209
Eco91I	GGTNACC	1 cut(s)	856
EcoICRI	GAGCTC	1 cut(s)	439
EcoO109I	RGGNCCY	1 cut(s)	845
EcoO65I	GGTNACC	1 cut(s)	856
EcoRII	CCWGG	3 cut(s)	282, 561, 1144
EcoRV	GATATC	1 cut(s)	1079
EcoT14I	CCWWGG	3 cut(s)	97, 271, 966
EcoT22I	ATGCAT	1 cut(s)	368
EcoT38I	GRGCYC	1 cut(s)	441
ErhI	CCWWGG	3 cut(s)	97, 271, 966
FaeI	CATG	7 cut(s)	101, 124, 370, 375, 988, 1126, 1354
FaqI	GGGAC	2 cut(s)	74, 1015
FatI	CATG	7 cut(s)	97, 120, 366, 371, 984, 1122, 1350
FauI	CCCGC	1 cut(s)	722
FauNDI	CATATG	1 cut(s)	526
FbaI	TGATCA	2 cut(s)	253, 987
FblI	GTMKAC	1 cut(s)	826
Fnu4HI	GCNGC	4 cut(s)	480, 622, 682, 1255
FokI	GGATG	2 cut(s)	1228, 1232
FriOI	GRGCYC	1 cut(s)	441
Fsp4HI	GCNGC	4 cut(s)	480, 622, 682, 1255
FspBI	CTAG	4 cut(s)	677, 1313, 1356, 1360
GlaI	GCGC	1 cut(s)	889
GluI	GCNGC	4 cut(s)	480, 622, 682, 1255
GsuI	CTGGAG	1 cut(s)	1103
HaeII	RGCGCY	1 cut(s)	891
HaeIII	GGCC	3 cut(s)	681, 971, 1149
HapII	CCGG	3 cut(s)	339, 549, 1022
HgaI	GACGC	1 cut(s)	16
HhaI	GCGC	1 cut(s)	890
Hin1I	GRCGYC	1 cut(s)	8
Hin1II	CATG	7 cut(s)	101, 124, 370, 375, 988, 1126, 1354
Hin6I	GCGC	1 cut(s)	888
HinP1I	GCGC	1 cut(s)	888
HincII	GTYRAC	3 cut(s)	168, 334, 827
HindII	GTYRAC	3 cut(s)	168, 334, 827
HinfI	GANTC	5 cut(s)	245, 581, 659, 995, 1266
HpaI	GTTAAC	1 cut(s)	168
HpaII	CCGG	3 cut(s)	339, 549, 1022
HphI	GGTGA	6 cut(s)	86, 298, 542, 850, 893, 1099
Hpy166II	GTNNAC	6 cut(s)	168, 206, 289, 305, 334, 827
Hpy188I	TCNGA	4 cut(s)	238, 897, 1101, 1265
Hpy188III	TCNNGA	6 cut(s)	131, 663, 848, 1082, 1110, 1123
Hpy8I	GTNNAC	6 cut(s)	168, 206, 289, 305, 334, 827
Hpy99I	CGWCG	3 cut(s)	10, 166, 204
HpyAV	CCTTC	1 cut(s)	1342
HpyCH4III	ACNGT	5 cut(s)	224, 571, 589, 1231, 1327
HpyCH4IV	ACGT	3 cut(s)	202, 208, 1013
HpyCH4V	TGCA	7 cut(s)	35, 366, 375, 767, 959, 1178, 1350
HpyF10VI	GCNNNNNNNGC	2 cut(s)	372, 1356
HpyF3I	CTNAG	5 cut(s)	61, 470, 894, 997, 1237
HpySE526I	ACGT	3 cut(s)	202, 208, 1013
Hsp92I	GRCGYC	1 cut(s)	8
Hsp92II	CATG	7 cut(s)	101, 124, 370, 375, 988, 1126, 1354
HspAI	GCGC	1 cut(s)	888
Ksp22I	TGATCA	2 cut(s)	253, 987
KspAI	GTTAAC	1 cut(s)	168
KspI	CCGCGG	1 cut(s)	685
Kzo9I	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
LmnI	GCTCC	4 cut(s)	476, 686, 935, 1186
Lsp1109I	GCAGC	2 cut(s)	608, 1266
LweI	GCATC	3 cut(s)	225, 946, 952
MabI	ACCWGGT	1 cut(s)	282
MaeI	CTAG	4 cut(s)	677, 1313, 1356, 1360
MaeII	ACGT	3 cut(s)	202, 208, 1013
MaeIII	GTNAC	6 cut(s)	131, 509, 530, 571, 856, 980
MalI	GATC	9 cut(s)	125, 213, 255, 348, 411, 656, 989, 1074, 1098
MboI	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
MboII	GAAGA	6 cut(s)	92, 135, 251, 397, 411, 1133
MflI	RGATCY	1 cut(s)	1096
MhlI	GDGCHC	2 cut(s)	441, 1285
MluCI	AATT	8 cut(s)	108, 157, 327, 463, 496, 666, 1053, 1276
MlyI	GAGTC	2 cut(s)	668, 989
MmeI	TCCRAC	4 cut(s)	477, 913, 1124, 1243
MnlI	CCTC	8 cut(s)	102, 125, 408, 411, 493, 814, 1054, 1078
Mph1103I	ATGCAT	1 cut(s)	368
MroXI	GAANNNNTTC	1 cut(s)	737
MseI	TTAA	6 cut(s)	167, 831, 975, 1010, 1068, 1170
MslI	CAYNNNNRTG	4 cut(s)	102, 633, 636, 1170
MspA1I	CMGCKG	2 cut(s)	684, 1257
MspI	CCGG	3 cut(s)	339, 549, 1022
MspR9I	CCNGG	3 cut(s)	284, 563, 1146
Mva1269I	GAATGC	1 cut(s)	1241
MvaI	CCWGG	3 cut(s)	284, 563, 1146
MvnI	CGCG	1 cut(s)	684
MwoI	GCNNNNNNNGC	2 cut(s)	372, 1356
NcoI	CCATGG	1 cut(s)	97
NdeI	CATATG	1 cut(s)	526
NdeII	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
NlaIII	CATG	7 cut(s)	101, 124, 370, 375, 988, 1126, 1354
NlaIV	GGNNCC	4 cut(s)	478, 756, 937, 1150
NmuCI	GTSAC	4 cut(s)	131, 509, 530, 856
NsiI	ATGCAT	1 cut(s)	368
NspI	RCATGY	1 cut(s)	370
PaeI	GCATGC	1 cut(s)	370
PagI	TCATGA	1 cut(s)	1122
PceI	AGGCCT	1 cut(s)	971
PcsI	WCGNNNNNNNCGW	1 cut(s)	220
PctI	GAATGC	1 cut(s)	1241
PdmI	GAANNNNTTC	1 cut(s)	737
PfeI	GAWTC	3 cut(s)	245, 581, 1266
PkrI	GCNGC	4 cut(s)	481, 623, 683, 1256
PleI	GAGTC	2 cut(s)	667, 989
PmaCI	CACGTG	1 cut(s)	209
PmlI	CACGTG	1 cut(s)	209
PpsI	GAGTC	2 cut(s)	667, 989
Ppu21I	YACGTR	1 cut(s)	209
PpuMI	RGGWCCY	1 cut(s)	845
Psp124BI	GAGCTC	1 cut(s)	441
Psp5II	RGGWCCY	1 cut(s)	845
Psp6I	CCWGG	3 cut(s)	282, 561, 1144
PspCI	CACGTG	1 cut(s)	209
PspEI	GGTNACC	1 cut(s)	856
PspGI	CCWGG	3 cut(s)	282, 561, 1144
PspN4I	GGNNCC	4 cut(s)	478, 756, 937, 1150
PspPI	GGNCC	2 cut(s)	845, 1148
PspPPI	RGGWCCY	1 cut(s)	845
PstNI	CAGNNNCTG	1 cut(s)	848
PsuI	RGATCY	1 cut(s)	1096
RsaI	GTAC	3 cut(s)	281, 304, 1016
RsaNI	GTAC	3 cut(s)	280, 303, 1015
RseI	CAYNNNNRTG	4 cut(s)	102, 633, 636, 1170
SacI	GAGCTC	1 cut(s)	441
SacII	CCGCGG	1 cut(s)	685
SalI	GTCGAC	1 cut(s)	825
SaqAI	TTAA	6 cut(s)	167, 831, 975, 1010, 1068, 1170
SatI	GCNGC	4 cut(s)	480, 622, 682, 1255
Sau3AI	GATC	9 cut(s)	123, 211, 253, 346, 409, 654, 987, 1072, 1096
Sau96I	GGNCC	2 cut(s)	845, 1148
SchI	GAGTC	2 cut(s)	668, 989
ScrFI	CCNGG	3 cut(s)	284, 563, 1146
SduI	GDGCHC	2 cut(s)	441, 1285
SexAI	ACCWGGT	1 cut(s)	282
SfaNI	GCATC	3 cut(s)	225, 946, 952
SfcI	CTRYAG	1 cut(s)	265
Sfr303I	CCGCGG	1 cut(s)	685
SgrBI	CCGCGG	1 cut(s)	685
SinI	GGWCC	1 cut(s)	845
SmiMI	CAYNNNNRTG	4 cut(s)	102, 633, 636, 1170
SmlI	CTYRAG	2 cut(s)	805, 911
SmoI	CTYRAG	2 cut(s)	805, 911
SphI	GCATGC	1 cut(s)	370
Sse9I	AATT	8 cut(s)	108, 157, 327, 463, 496, 666, 1053, 1276
SseBI	AGGCCT	1 cut(s)	971
SsiI	CCGC	7 cut(s)	474, 480, 624, 682, 684, 729, 1257
SspI	AATATT	2 cut(s)	521, 737
SspMI	CTAG	4 cut(s)	677, 1313, 1356, 1360
SstI	GAGCTC	1 cut(s)	441
StuI	AGGCCT	1 cut(s)	971
StyD4I	CCNGG	3 cut(s)	282, 561, 1144
StyI	CCWWGG	3 cut(s)	97, 271, 966
TaaI	ACNGT	5 cut(s)	224, 571, 589, 1231, 1327
TaiI	ACGT	3 cut(s)	205, 211, 1016
TaqI	TCGA	6 cut(s)	387, 408, 657, 826, 875, 1075
TasI	AATT	8 cut(s)	108, 157, 327, 463, 496, 666, 1053, 1276
TauI	GCSGC	2 cut(s)	482, 684
TfiI	GAWTC	3 cut(s)	245, 581, 1266
Tru1I	TTAA	6 cut(s)	167, 831, 975, 1010, 1068, 1170
Tru9I	TTAA	6 cut(s)	167, 831, 975, 1010, 1068, 1170
TscAI	CASTG	3 cut(s)	574, 592, 655
TseFI	GTSAC	4 cut(s)	131, 509, 530, 856
TseI	GCWGC	2 cut(s)	621, 1254
Tsp45I	GTSAC	4 cut(s)	131, 509, 530, 856
TspDTI	ATGAA	7 cut(s)	217, 258, 310, 411, 513, 828, 1111
TspRI	CASTG	3 cut(s)	574, 592, 655
VpaK11BI	GGWCC	1 cut(s)	845
XceI	RCATGY	1 cut(s)	370
XmiI	GTMKAC	1 cut(s)	826
XmnI	GAANNNNTTC	1 cut(s)	737
XspI	CTAG	4 cut(s)	677, 1313, 1356, 1360
Zsp2I	ATGCAT	1 cut(s)	368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.