Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 78 |
| Acc16I | TGCGCA | 1 cut(s) | 1306 |
| Acc36I | ACCTGC | 1 cut(s) | 227 |
| AccBSI | CCGCTC | 1 cut(s) | 280 |
| AccI | GTMKAC | 2 cut(s) | 433, 469 |
| AciI | CCGC | 1 cut(s) | 278 |
| AclWI | GGATC | 2 cut(s) | 223, 743 |
| AcsI | RAATTY | 3 cut(s) | 139, 179, 1191 |
| AcyI | GRCGYC | 1 cut(s) | 1082 |
| AfaI | GTAC | 1 cut(s) | 955 |
| AfeI | AGCGCT | 1 cut(s) | 121 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 347, 713, 1117 |
| AgsI | TTSAA | 4 cut(s) | 639, 836, 1163, 1197 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 333 |
| AjiI | CACGTC | 1 cut(s) | 674 |
| AjnI | CCWGG | 2 cut(s) | 211, 1171 |
| AluBI | AGCT | 2 cut(s) | 501, 1006 |
| AluI | AGCT | 2 cut(s) | 501, 1006 |
| Alw26I | GTCTC | 2 cut(s) | 420, 1064 |
| AlwI | GGATC | 2 cut(s) | 223, 743 |
| Ama87I | CYCGRG | 1 cut(s) | 382 |
| Aor51HI | AGCGCT | 1 cut(s) | 121 |
| AoxI | GGCC | 1 cut(s) | 677 |
| ApeKI | GCWGC | 1 cut(s) | 312 |
| ApoI | RAATTY | 3 cut(s) | 139, 179, 1191 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 139 |
| AspLEI | GCGC | 3 cut(s) | 122, 248, 1307 |
| AspS9I | GGNCC | 3 cut(s) | 200, 677, 1147 |
| AsuHPI | GGTGA | 5 cut(s) | 43, 144, 210, 812, 823 |
| AvaI | CYCGRG | 1 cut(s) | 382 |
| AvaII | GGWCC | 2 cut(s) | 200, 1147 |
| BbvI | GCAGC | 1 cut(s) | 324 |
| BccI | CCATC | 4 cut(s) | 190, 887, 988, 1138 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 275, 309 |
| BciT130I | CCWGG | 2 cut(s) | 213, 1173 |
| BcoDI | GTCTC | 2 cut(s) | 420, 1064 |
| BfaI | CTAG | 1 cut(s) | 512 |
| BfmI | CTRYAG | 4 cut(s) | 87, 430, 570, 1238 |
| BfoI | RGCGCY | 1 cut(s) | 123 |
| BfuAI | ACCTGC | 1 cut(s) | 227 |
| BglII | AGATCT | 1 cut(s) | 768 |
| BisI | GCNGC | 1 cut(s) | 313 |
| BlsI | GCNGC | 1 cut(s) | 314 |
| Bme1390I | CCNGG | 2 cut(s) | 213, 1173 |
| Bme18I | GGWCC | 2 cut(s) | 200, 1147 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 333 |
| BmeT110I | CYCGRG | 1 cut(s) | 382 |
| BmgBI | CACGTC | 1 cut(s) | 674 |
| BmgT120I | GGNCC | 3 cut(s) | 200, 677, 1147 |
| BmiI | GGNNCC | 2 cut(s) | 201, 274 |
| BmrFI | CCNGG | 2 cut(s) | 213, 1173 |
| BmrI | ACTGGG | 1 cut(s) | 921 |
| BmsI | GCATC | 1 cut(s) | 223 |
| BmuI | ACTGGG | 1 cut(s) | 921 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1186 |
| BpuEI | CTTGAG | 1 cut(s) | 908 |
| BsaHI | GRCGYC | 1 cut(s) | 1082 |
| BsaJI | CCNNGG | 3 cut(s) | 439, 1044, 1172 |
| BsaWI | WCCGGW | 1 cut(s) | 990 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1069, 1099 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 347, 713, 1117 |
| Bse118I | RCCGGY | 1 cut(s) | 1135 |
| Bse1I | ACTGG | 2 cut(s) | 526, 916 |
| Bse3DI | GCAATG | 2 cut(s) | 155, 338 |
| BseBI | CCWGG | 2 cut(s) | 213, 1173 |
| BseDI | CCNNGG | 3 cut(s) | 439, 1044, 1172 |
| BseGI | GGATG | 3 cut(s) | 563, 756, 999 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 347, 713, 1117 |
| BseMI | GCAATG | 2 cut(s) | 155, 338 |
| BseMII | CTCAG | 1 cut(s) | 1016 |
| BseNI | ACTGG | 2 cut(s) | 526, 916 |
| BseXI | GCAGC | 1 cut(s) | 324 |
| BshFI | GGCC | 1 cut(s) | 679 |
| BsiHKCI | CYCGRG | 1 cut(s) | 382 |
| BsiSI | CCGG | 2 cut(s) | 991, 1136 |
| BslFI | GGGAC | 2 cut(s) | 532, 928 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 347, 713, 1117 |
| BsmAI | GTCTC | 2 cut(s) | 420, 1064 |
| BsmFI | GGGAC | 2 cut(s) | 532, 928 |
| BsnI | GGCC | 1 cut(s) | 679 |
| BsoBI | CYCGRG | 1 cut(s) | 382 |
| Bsp143I | GATC | 4 cut(s) | 215, 316, 748, 768 |
| BspACI | CCGC | 1 cut(s) | 278 |
| BspANI | GGCC | 1 cut(s) | 679 |
| BspCNI | CTCAG | 1 cut(s) | 1015 |
| BspLI | GGNNCC | 2 cut(s) | 201, 274 |
| BspMI | ACCTGC | 1 cut(s) | 227 |
| BspPI | GGATC | 2 cut(s) | 223, 743 |
| BspQI | GCTCTTC | 1 cut(s) | 287 |
| BsrBI | CCGCTC | 1 cut(s) | 280 |
| BsrDI | GCAATG | 2 cut(s) | 155, 338 |
| BsrFI | RCCGGY | 1 cut(s) | 1135 |
| BsrI | ACTGG | 2 cut(s) | 526, 916 |
| BssAI | RCCGGY | 1 cut(s) | 1135 |
| BssECI | CCNNGG | 3 cut(s) | 439, 1044, 1172 |
| BssMI | GATC | 4 cut(s) | 215, 316, 748, 768 |
| BssNAI | GTATAC | 1 cut(s) | 470 |
| BssNI | GRCGYC | 1 cut(s) | 1082 |
| BssT1I | CCWWGG | 2 cut(s) | 439, 1044 |
| Bst1107I | GTATAC | 1 cut(s) | 470 |
| Bst2UI | CCWGG | 2 cut(s) | 213, 1173 |
| Bst4CI | ACNGT | 7 cut(s) | 91, 192, 431, 553, 851, 865, 950 |
| Bst6I | CTCTTC | 1 cut(s) | 287 |
| BstACI | GRCGYC | 1 cut(s) | 1082 |
| BstDEI | CTNAG | 2 cut(s) | 1002, 1186 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 345 |
| BstF5I | GGATG | 3 cut(s) | 563, 756, 999 |
| BstH2I | RGCGCY | 1 cut(s) | 123 |
| BstHHI | GCGC | 3 cut(s) | 122, 248, 1307 |
| BstKTI | GATC | 4 cut(s) | 218, 319, 751, 771 |
| BstMAI | GTCTC | 2 cut(s) | 420, 1064 |
| BstMBI | GATC | 4 cut(s) | 215, 316, 748, 768 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 92, 337, 360, 369 |
| BstNI | CCWGG | 2 cut(s) | 213, 1173 |
| BstSCI | CCNGG | 2 cut(s) | 211, 1171 |
| BstSFI | CTRYAG | 4 cut(s) | 87, 430, 570, 1238 |
| BstV1I | GCAGC | 1 cut(s) | 324 |
| BstX2I | RGATCY | 2 cut(s) | 748, 768 |
| BstYI | RGATCY | 2 cut(s) | 748, 768 |
| BstZ17I | GTATAC | 1 cut(s) | 470 |
| BsuRI | GGCC | 1 cut(s) | 679 |
| BtgZI | GCGATG | 1 cut(s) | 1157 |
| BtrI | CACGTC | 1 cut(s) | 674 |
| BtsCI | GGATG | 3 cut(s) | 563, 756, 999 |
| BtsIMutI | CAGTG | 1 cut(s) | 955 |
| BveI | ACCTGC | 1 cut(s) | 227 |
| CfoI | GCGC | 3 cut(s) | 122, 248, 1307 |
| Cfr10I | RCCGGY | 1 cut(s) | 1135 |
| Cfr13I | GGNCC | 3 cut(s) | 200, 677, 1147 |
| CseI | GACGC | 2 cut(s) | 337, 1090 |
| Csp6I | GTAC | 1 cut(s) | 954 |
| CviAII | CATG | 2 cut(s) | 458, 937 |
| CviQI | GTAC | 1 cut(s) | 954 |
| DdeI | CTNAG | 2 cut(s) | 1002, 1186 |
| DpnI | GATC | 4 cut(s) | 217, 318, 750, 770 |
| DpnII | GATC | 4 cut(s) | 215, 316, 748, 768 |
| DriI | GACNNNNNGTC | 1 cut(s) | 333 |
| Eam1104I | CTCTTC | 1 cut(s) | 287 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 333 |
| EarI | CTCTTC | 1 cut(s) | 287 |
| Eco130I | CCWWGG | 2 cut(s) | 439, 1044 |
| Eco32I | GATATC | 1 cut(s) | 1284 |
| Eco47I | GGWCC | 2 cut(s) | 200, 1147 |
| Eco47III | AGCGCT | 1 cut(s) | 121 |
| Eco88I | CYCGRG | 1 cut(s) | 382 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 345 |
| EcoRII | CCWGG | 2 cut(s) | 211, 1171 |
| EcoRV | GATATC | 1 cut(s) | 1284 |
| EcoT14I | CCWWGG | 2 cut(s) | 439, 1044 |
| ErhI | CCWWGG | 2 cut(s) | 439, 1044 |
| FaeI | CATG | 2 cut(s) | 461, 940 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 350, 382, 715, 747 |
| FaqI | GGGAC | 2 cut(s) | 532, 928 |
| FatI | CATG | 2 cut(s) | 457, 936 |
| FauI | CCCGC | 1 cut(s) | 285 |
| FblI | GTMKAC | 2 cut(s) | 433, 469 |
| Fnu4HI | GCNGC | 1 cut(s) | 313 |
| FokI | GGATG | 3 cut(s) | 550, 743, 1006 |
| Fsp4HI | GCNGC | 1 cut(s) | 313 |
| FspBI | CTAG | 1 cut(s) | 512 |
| FspI | TGCGCA | 1 cut(s) | 1306 |
| GlaI | GCGC | 3 cut(s) | 121, 247, 1306 |
| GluI | GCNGC | 1 cut(s) | 313 |
| HaeII | RGCGCY | 1 cut(s) | 123 |
| HaeIII | GGCC | 1 cut(s) | 679 |
| HapII | CCGG | 2 cut(s) | 991, 1136 |
| HgaI | GACGC | 2 cut(s) | 337, 1090 |
| HhaI | GCGC | 3 cut(s) | 122, 248, 1307 |
| Hin1I | GRCGYC | 1 cut(s) | 1082 |
| Hin1II | CATG | 2 cut(s) | 461, 940 |
| Hin6I | GCGC | 3 cut(s) | 120, 246, 1305 |
| HinP1I | GCGC | 3 cut(s) | 120, 246, 1305 |
| HincII | GTYRAC | 1 cut(s) | 946 |
| HindII | GTYRAC | 1 cut(s) | 946 |
| HinfI | GANTC | 6 cut(s) | 435, 629, 726, 836, 1057, 1265 |
| HpaII | CCGG | 2 cut(s) | 991, 1136 |
| HphI | GGTGA | 5 cut(s) | 43, 144, 210, 812, 823 |
| Hpy166II | GTNNAC | 5 cut(s) | 434, 470, 496, 946, 971 |
| Hpy188I | TCNGA | 3 cut(s) | 134, 1229, 1270 |
| Hpy188III | TCNNGA | 5 cut(s) | 203, 423, 512, 925, 1252 |
| Hpy8I | GTNNAC | 5 cut(s) | 434, 470, 496, 946, 971 |
| Hpy99I | CGWCG | 2 cut(s) | 192, 1084 |
| HpyAV | CCTTC | 2 cut(s) | 509, 797 |
| HpyCH4III | ACNGT | 7 cut(s) | 91, 192, 431, 553, 851, 865, 950 |
| HpyCH4IV | ACGT | 3 cut(s) | 673, 962, 967 |
| HpyCH4V | TGCA | 7 cut(s) | 160, 226, 236, 296, 455, 745, 1027 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 92, 337, 360, 369 |
| HpyF3I | CTNAG | 2 cut(s) | 1002, 1186 |
| HpySE526I | ACGT | 3 cut(s) | 673, 962, 967 |
| Hsp92I | GRCGYC | 1 cut(s) | 1082 |
| Hsp92II | CATG | 2 cut(s) | 461, 940 |
| HspAI | GCGC | 3 cut(s) | 120, 246, 1305 |
| Kzo9I | GATC | 4 cut(s) | 215, 316, 748, 768 |
| LguI | GCTCTTC | 1 cut(s) | 287 |
| LmnI | GCTCC | 1 cut(s) | 709 |
| Lsp1109I | GCAGC | 1 cut(s) | 324 |
| LweI | GCATC | 1 cut(s) | 223 |
| MaeI | CTAG | 1 cut(s) | 512 |
| MaeII | ACGT | 3 cut(s) | 673, 962, 967 |
| MaeIII | GTNAC | 5 cut(s) | 553, 811, 932, 963, 997 |
| MalI | GATC | 4 cut(s) | 217, 318, 750, 770 |
| MbiI | CCGCTC | 1 cut(s) | 280 |
| MboI | GATC | 4 cut(s) | 215, 316, 748, 768 |
| MboII | GAAGA | 2 cut(s) | 274, 1175 |
| MfeI | CAATTG | 1 cut(s) | 297 |
| MflI | RGATCY | 2 cut(s) | 748, 768 |
| MlyI | GAGTC | 3 cut(s) | 429, 1066, 1259 |
| MmeI | TCCRAC | 1 cut(s) | 694 |
| MnlI | CCTC | 5 cut(s) | 403, 710, 1095, 1111, 1223 |
| MroXI | GAANNNNTTC | 1 cut(s) | 139 |
| MseI | TTAA | 6 cut(s) | 903, 1020, 1071, 1094, 1290, 1314 |
| MspI | CCGG | 2 cut(s) | 991, 1136 |
| MspR9I | CCNGG | 2 cut(s) | 213, 1173 |
| MunI | CAATTG | 1 cut(s) | 297 |
| MvaI | CCWGG | 2 cut(s) | 213, 1173 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 92, 337, 360, 369 |
| NdeII | GATC | 4 cut(s) | 215, 316, 748, 768 |
| NlaIII | CATG | 2 cut(s) | 461, 940 |
| NlaIV | GGNNCC | 2 cut(s) | 201, 274 |
| NmuCI | GTSAC | 2 cut(s) | 811, 963 |
| NsbI | TGCGCA | 1 cut(s) | 1306 |
| PciSI | GCTCTTC | 1 cut(s) | 287 |
| PdmI | GAANNNNTTC | 1 cut(s) | 139 |
| PfeI | GAWTC | 3 cut(s) | 629, 726, 836 |
| PkrI | GCNGC | 1 cut(s) | 314 |
| PleI | GAGTC | 3 cut(s) | 429, 1065, 1259 |
| PpsI | GAGTC | 3 cut(s) | 429, 1065, 1259 |
| PsiI | TTATAA | 1 cut(s) | 78 |
| Psp6I | CCWGG | 2 cut(s) | 211, 1171 |
| PspGI | CCWGG | 2 cut(s) | 211, 1171 |
| PspN4I | GGNNCC | 2 cut(s) | 201, 274 |
| PspPI | GGNCC | 3 cut(s) | 200, 677, 1147 |
| PsuI | RGATCY | 2 cut(s) | 748, 768 |
| RsaI | GTAC | 1 cut(s) | 955 |
| RsaNI | GTAC | 1 cut(s) | 954 |
| SapI | GCTCTTC | 1 cut(s) | 287 |
| SaqAI | TTAA | 6 cut(s) | 903, 1020, 1071, 1094, 1290, 1314 |
| SatI | GCNGC | 1 cut(s) | 313 |
| Sau3AI | GATC | 4 cut(s) | 215, 316, 748, 768 |
| Sau96I | GGNCC | 3 cut(s) | 200, 677, 1147 |
| SchI | GAGTC | 3 cut(s) | 429, 1066, 1259 |
| ScrFI | CCNGG | 2 cut(s) | 213, 1173 |
| SfaNI | GCATC | 1 cut(s) | 223 |
| SfcI | CTRYAG | 4 cut(s) | 87, 430, 570, 1238 |
| SinI | GGWCC | 2 cut(s) | 200, 1147 |
| SmlI | CTYRAG | 1 cut(s) | 923 |
| SmoI | CTYRAG | 1 cut(s) | 923 |
| SsiI | CCGC | 1 cut(s) | 278 |
| SspMI | CTAG | 1 cut(s) | 512 |
| StyD4I | CCNGG | 2 cut(s) | 211, 1171 |
| StyI | CCWWGG | 2 cut(s) | 439, 1044 |
| TaaI | ACNGT | 7 cut(s) | 91, 192, 431, 553, 851, 865, 950 |
| TaiI | ACGT | 3 cut(s) | 676, 965, 970 |
| TaqI | TCGA | 5 cut(s) | 187, 231, 285, 828, 882 |
| TaqII | GACCGA | 1 cut(s) | 408 |
| TfiI | GAWTC | 3 cut(s) | 629, 726, 836 |
| Tru1I | TTAA | 6 cut(s) | 903, 1020, 1071, 1094, 1290, 1314 |
| Tru9I | TTAA | 6 cut(s) | 903, 1020, 1071, 1094, 1290, 1314 |
| TscAI | CASTG | 1 cut(s) | 955 |
| TseFI | GTSAC | 2 cut(s) | 811, 963 |
| TseI | GCWGC | 1 cut(s) | 312 |
| Tsp45I | GTSAC | 2 cut(s) | 811, 963 |
| TspDTI | ATGAA | 5 cut(s) | 56, 272, 642, 953, 1024 |
| TspRI | CASTG | 1 cut(s) | 955 |
| VpaK11BI | GGWCC | 2 cut(s) | 200, 1147 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 345 |
| XapI | RAATTY | 3 cut(s) | 139, 179, 1191 |
| XbaI | TCTAGA | 1 cut(s) | 511 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1118 |
| XmiI | GTMKAC | 2 cut(s) | 433, 469 |
| XmnI | GAANNNNTTC | 1 cut(s) | 139 |
| XspI | CTAG | 1 cut(s) | 512 |