Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 432 |
| AccI | GTMKAC | 2 cut(s) | 333, 817 |
| AciI | CCGC | 5 cut(s) | 99, 339, 615, 717, 1092 |
| AclWI | GGATC | 3 cut(s) | 115, 404, 1076 |
| AcoI | YGGCCR | 1 cut(s) | 1185 |
| AcsI | RAATTY | 1 cut(s) | 1180 |
| AdeI | CACNNNGTG | 1 cut(s) | 251 |
| AfaI | GTAC | 3 cut(s) | 281, 304, 1001 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 95, 323, 699, 700, 1091 |
| AgsI | TTSAA | 3 cut(s) | 388, 501, 905 |
| AjiI | CACGTC | 1 cut(s) | 206 |
| AjnI | CCWGG | 4 cut(s) | 247, 282, 838, 1284 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1133, 1165 |
| AluBI | AGCT | 6 cut(s) | 437, 504, 521, 677, 901, 1177 |
| AluI | AGCT | 6 cut(s) | 437, 504, 521, 677, 901, 1177 |
| Alw21I | GWGCWC | 2 cut(s) | 256, 377 |
| Alw26I | GTCTC | 4 cut(s) | 232, 796, 1208, 1321 |
| Alw44I | GTGCAC | 1 cut(s) | 373 |
| AlwI | GGATC | 3 cut(s) | 115, 404, 1076 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 839 |
| AoxI | GGCC | 3 cut(s) | 667, 1135, 1185 |
| ApaLI | GTGCAC | 1 cut(s) | 373 |
| ApeKI | GCWGC | 1 cut(s) | 1242 |
| ApoI | RAATTY | 1 cut(s) | 1180 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 725 |
| AspS9I | GGNCC | 2 cut(s) | 836, 1136 |
| AsuHPI | GGTGA | 3 cut(s) | 237, 298, 1084 |
| AsuII | TTCGAA | 1 cut(s) | 1029 |
| AvaII | GGWCC | 1 cut(s) | 836 |
| AxyI | CCTNAGG | 1 cut(s) | 1237 |
| BaeGI | GKGCMC | 1 cut(s) | 377 |
| BauI | CACGAG | 1 cut(s) | 442 |
| BbsI | GAAGAC | 1 cut(s) | 43 |
| Bbv12I | GWGCWC | 2 cut(s) | 256, 377 |
| BbvI | GCAGC | 1 cut(s) | 1254 |
| BccI | CCATC | 1 cut(s) | 145 |
| BceAI | ACGGC | 2 cut(s) | 355, 654 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 898, 932 |
| BciT130I | CCWGG | 4 cut(s) | 249, 284, 840, 1286 |
| BclI | TGATCA | 1 cut(s) | 972 |
| BcoDI | GTCTC | 4 cut(s) | 232, 796, 1208, 1321 |
| BfaI | CTAG | 2 cut(s) | 665, 1301 |
| BfmI | CTRYAG | 1 cut(s) | 265 |
| BglII | AGATCT | 1 cut(s) | 1099 |
| BisI | GCNGC | 1 cut(s) | 1243 |
| BlpI | GCTNAGC | 1 cut(s) | 1225 |
| BlsI | GCNGC | 1 cut(s) | 1244 |
| Bme1390I | CCNGG | 4 cut(s) | 249, 284, 840, 1286 |
| Bme18I | GGWCC | 1 cut(s) | 836 |
| BmgBI | CACGTC | 1 cut(s) | 206 |
| BmgT120I | GGNCC | 2 cut(s) | 836, 1136 |
| BmiI | GGNNCC | 3 cut(s) | 744, 922, 1138 |
| BmrFI | CCNGG | 4 cut(s) | 249, 284, 840, 1286 |
| BmrI | ACTGGG | 1 cut(s) | 648 |
| BmsI | GCATC | 4 cut(s) | 145, 458, 931, 937 |
| BmuI | ACTGGG | 1 cut(s) | 648 |
| BpiI | GAAGAC | 1 cut(s) | 43 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1035, 1067 |
| BpmI | CTGGAG | 2 cut(s) | 861, 1088 |
| Bpu10I | CCTNAGC | 1 cut(s) | 863 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1225 |
| Bpu14I | TTCGAA | 1 cut(s) | 1029 |
| BpuEI | CTTGAG | 1 cut(s) | 917 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1062 |
| BsaJI | CCNNGG | 2 cut(s) | 670, 951 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 95, 323, 699, 700, 1091 |
| Bse1I | ACTGG | 6 cut(s) | 492, 582, 643, 769, 788, 1093 |
| Bse21I | CCTNAGG | 1 cut(s) | 1237 |
| Bse3DI | GCAATG | 1 cut(s) | 33 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1062 |
| BseBI | CCWGG | 4 cut(s) | 249, 284, 840, 1286 |
| BseDI | CCNNGG | 2 cut(s) | 670, 951 |
| BseGI | GGATG | 2 cut(s) | 449, 1213 |
| BseJI | GATNNNNATC | 1 cut(s) | 1062 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 95, 323, 699, 700, 1091 |
| BseMI | GCAATG | 1 cut(s) | 33 |
| BseMII | CTCAG | 2 cut(s) | 996, 1251 |
| BseNI | ACTGG | 6 cut(s) | 492, 582, 643, 769, 788, 1093 |
| BseRI | GAGGAG | 3 cut(s) | 78, 390, 1225 |
| BseSI | GKGCMC | 1 cut(s) | 377 |
| BseXI | GCAGC | 1 cut(s) | 1254 |
| BsgI | GTGCAG | 1 cut(s) | 738 |
| BshFI | GGCC | 3 cut(s) | 669, 1137, 1187 |
| BsiHKAI | GWGCWC | 2 cut(s) | 256, 377 |
| BsiSI | CCGG | 2 cut(s) | 413, 1007 |
| BslFI | GGGAC | 2 cut(s) | 544, 1000 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 95, 323, 699, 700, 1091 |
| BsmAI | GTCTC | 4 cut(s) | 232, 796, 1208, 1321 |
| BsmBI | CGTCTC | 1 cut(s) | 232 |
| BsmFI | GGGAC | 2 cut(s) | 544, 1000 |
| BsmI | GAATGC | 1 cut(s) | 1229 |
| BsnI | GGCC | 3 cut(s) | 669, 1137, 1187 |
| Bsp119I | TTCGAA | 1 cut(s) | 1029 |
| Bsp1286I | GDGCHC | 2 cut(s) | 256, 377 |
| Bsp143I | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1225 |
| BspACI | CCGC | 5 cut(s) | 99, 339, 615, 717, 1092 |
| BspANI | GGCC | 3 cut(s) | 669, 1137, 1187 |
| BspCNI | CTCAG | 2 cut(s) | 995, 1250 |
| BspLI | GGNNCC | 3 cut(s) | 744, 922, 1138 |
| BspPI | GGATC | 3 cut(s) | 115, 404, 1076 |
| BspT104I | TTCGAA | 1 cut(s) | 1029 |
| BsrDI | GCAATG | 1 cut(s) | 33 |
| BsrI | ACTGG | 6 cut(s) | 492, 582, 643, 769, 788, 1093 |
| BssECI | CCNNGG | 2 cut(s) | 670, 951 |
| BssMI | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| BssSI | CACGAG | 1 cut(s) | 442 |
| BssT1I | CCWWGG | 1 cut(s) | 951 |
| Bst2BI | CACGAG | 1 cut(s) | 442 |
| Bst2UI | CCWGG | 4 cut(s) | 249, 284, 840, 1286 |
| Bst4CI | ACNGT | 7 cut(s) | 95, 128, 221, 571, 937, 1219, 1315 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 372 |
| BstBI | TTCGAA | 1 cut(s) | 1029 |
| BstC8I | GCNNGC | 2 cut(s) | 368, 1135 |
| BstDEI | CTNAG | 5 cut(s) | 58, 863, 982, 1225, 1237 |
| BstDSI | CCRYGG | 1 cut(s) | 670 |
| BstEII | GGTNACC | 1 cut(s) | 847 |
| BstF5I | GGATG | 2 cut(s) | 449, 1213 |
| BstKTI | GATC | 7 cut(s) | 123, 349, 412, 975, 1060, 1084, 1102 |
| BstMAI | GTCTC | 4 cut(s) | 232, 796, 1208, 1321 |
| BstMBI | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 372, 510 |
| BstNI | CCWGG | 4 cut(s) | 249, 284, 840, 1286 |
| BstNSI | RCATGY | 1 cut(s) | 370 |
| BstPI | GGTNACC | 1 cut(s) | 847 |
| BstSCI | CCNGG | 4 cut(s) | 247, 282, 838, 1284 |
| BstSFI | CTRYAG | 1 cut(s) | 265 |
| BstSLI | GKGCMC | 1 cut(s) | 377 |
| BstV1I | GCAGC | 1 cut(s) | 1254 |
| BstV2I | GAAGAC | 1 cut(s) | 43 |
| BstX2I | RGATCY | 2 cut(s) | 1081, 1099 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1160 |
| BstYI | RGATCY | 2 cut(s) | 1081, 1099 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1237 |
| BsuRI | GGCC | 3 cut(s) | 669, 1137, 1187 |
| BtgI | CCRYGG | 1 cut(s) | 670 |
| BtrI | CACGTC | 1 cut(s) | 206 |
| BtsCI | GGATG | 2 cut(s) | 449, 1213 |
| BtsI | GCAGTG | 1 cut(s) | 277 |
| BtsIMutI | CAGTG | 4 cut(s) | 100, 277, 575, 636 |
| Cac8I | GCNNGC | 2 cut(s) | 368, 1135 |
| CaiI | CAGNNNCTG | 1 cut(s) | 839 |
| Cfr13I | GGNCC | 2 cut(s) | 836, 1136 |
| CsiI | ACCWGGT | 3 cut(s) | 247, 282, 1284 |
| Csp6I | GTAC | 3 cut(s) | 280, 303, 1000 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 16, 51 |
| CviAII | CATG | 3 cut(s) | 118, 367, 970 |
| CviQI | GTAC | 3 cut(s) | 280, 303, 1000 |
| DdeI | CTNAG | 5 cut(s) | 58, 863, 982, 1225, 1237 |
| DpnI | GATC | 7 cut(s) | 122, 348, 411, 974, 1059, 1083, 1101 |
| DpnII | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| DraIII | CACNNNGTG | 1 cut(s) | 251 |
| EaeI | YGGCCR | 1 cut(s) | 1185 |
| Eco130I | CCWWGG | 1 cut(s) | 951 |
| Eco32I | GATATC | 1 cut(s) | 588 |
| Eco47I | GGWCC | 1 cut(s) | 836 |
| Eco81I | CCTNAGG | 1 cut(s) | 1237 |
| Eco91I | GGTNACC | 1 cut(s) | 847 |
| EcoO109I | RGGNCCY | 1 cut(s) | 836 |
| EcoO65I | GGTNACC | 1 cut(s) | 847 |
| EcoRII | CCWGG | 4 cut(s) | 247, 282, 838, 1284 |
| EcoRV | GATATC | 1 cut(s) | 588 |
| EcoT14I | CCWWGG | 1 cut(s) | 951 |
| EcoT22I | ATGCAT | 1 cut(s) | 368 |
| ErhI | CCWWGG | 1 cut(s) | 951 |
| Esp3I | CGTCTC | 1 cut(s) | 232 |
| FaeI | CATG | 3 cut(s) | 121, 370, 973 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 468, 500 |
| FaqI | GGGAC | 2 cut(s) | 544, 1000 |
| FatI | CATG | 3 cut(s) | 117, 366, 969 |
| FauI | CCCGC | 1 cut(s) | 710 |
| FauNDI | CATATG | 1 cut(s) | 526 |
| FbaI | TGATCA | 1 cut(s) | 972 |
| FblI | GTMKAC | 2 cut(s) | 333, 817 |
| Fnu4HI | GCNGC | 1 cut(s) | 1243 |
| FokI | GGATG | 2 cut(s) | 436, 1220 |
| Fsp4HI | GCNGC | 1 cut(s) | 1243 |
| FspBI | CTAG | 2 cut(s) | 665, 1301 |
| GluI | GCNGC | 1 cut(s) | 1243 |
| GsuI | CTGGAG | 2 cut(s) | 861, 1088 |
| HaeIII | GGCC | 3 cut(s) | 669, 1137, 1187 |
| HapII | CCGG | 2 cut(s) | 413, 1007 |
| Hin1II | CATG | 3 cut(s) | 121, 370, 973 |
| HincII | GTYRAC | 3 cut(s) | 165, 334, 818 |
| HindII | GTYRAC | 3 cut(s) | 165, 334, 818 |
| HindIII | AAGCTT | 1 cut(s) | 519 |
| HinfI | GANTC | 9 cut(s) | 170, 242, 581, 647, 819, 868, 980, 1010, 1254 |
| HpaI | GTTAAC | 1 cut(s) | 165 |
| HpaII | CCGG | 2 cut(s) | 413, 1007 |
| HphI | GGTGA | 3 cut(s) | 237, 298, 1084 |
| Hpy166II | GTNNAC | 8 cut(s) | 165, 305, 334, 375, 472, 636, 818, 1018 |
| Hpy188I | TCNGA | 4 cut(s) | 235, 920, 1086, 1099 |
| Hpy188III | TCNNGA | 2 cut(s) | 651, 1067 |
| Hpy8I | GTNNAC | 8 cut(s) | 165, 305, 334, 375, 472, 636, 818, 1018 |
| Hpy99I | CGWCG | 1 cut(s) | 536 |
| HpyAV | CCTTC | 2 cut(s) | 895, 1330 |
| HpyCH4III | ACNGT | 7 cut(s) | 95, 128, 221, 571, 937, 1219, 1315 |
| HpyCH4IV | ACGT | 3 cut(s) | 205, 761, 998 |
| HpyCH4V | TGCA | 6 cut(s) | 74, 196, 366, 375, 449, 755 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 372, 510 |
| HpyF3I | CTNAG | 5 cut(s) | 58, 863, 982, 1225, 1237 |
| HpySE526I | ACGT | 3 cut(s) | 205, 761, 998 |
| Hsp92II | CATG | 3 cut(s) | 121, 370, 973 |
| Ksp22I | TGATCA | 1 cut(s) | 972 |
| KspAI | GTTAAC | 1 cut(s) | 165 |
| Kzo9I | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| LmnI | GCTCC | 3 cut(s) | 91, 674, 920 |
| Lsp1109I | GCAGC | 1 cut(s) | 1254 |
| LweI | GCATC | 4 cut(s) | 145, 458, 931, 937 |
| MabI | ACCWGGT | 3 cut(s) | 247, 282, 1284 |
| MaeI | CTAG | 2 cut(s) | 665, 1301 |
| MaeII | ACGT | 3 cut(s) | 205, 761, 998 |
| MaeIII | GTNAC | 5 cut(s) | 128, 571, 847, 1106, 1144 |
| MalI | GATC | 7 cut(s) | 122, 348, 411, 974, 1059, 1083, 1101 |
| MboI | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| MboII | GAAGA | 4 cut(s) | 43, 132, 248, 411 |
| MflI | RGATCY | 2 cut(s) | 1081, 1099 |
| MhlI | GDGCHC | 2 cut(s) | 256, 377 |
| MluCI | AATT | 7 cut(s) | 327, 496, 654, 1038, 1180, 1192, 1279 |
| MlyI | GAGTC | 4 cut(s) | 656, 813, 974, 1019 |
| MmeI | TCCRAC | 4 cut(s) | 477, 640, 898, 1109 |
| MnlI | CCTC | 9 cut(s) | 65, 99, 122, 408, 411, 1003, 1039, 1063, 1246 |
| Mph1103I | ATGCAT | 1 cut(s) | 368 |
| MroXI | GAANNNNTTC | 1 cut(s) | 725 |
| MseI | TTAA | 3 cut(s) | 164, 960, 1158 |
| MslI | CAYNNNNRTG | 2 cut(s) | 624, 1158 |
| MspA1I | CMGCKG | 1 cut(s) | 339 |
| MspI | CCGG | 2 cut(s) | 413, 1007 |
| MspR9I | CCNGG | 4 cut(s) | 249, 284, 840, 1286 |
| Mva1269I | GAATGC | 1 cut(s) | 1229 |
| MvaI | CCWGG | 4 cut(s) | 249, 284, 840, 1286 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 372, 510 |
| NdeI | CATATG | 1 cut(s) | 526 |
| NdeII | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| NlaIII | CATG | 3 cut(s) | 121, 370, 973 |
| NlaIV | GGNNCC | 3 cut(s) | 744, 922, 1138 |
| NmuCI | GTSAC | 2 cut(s) | 128, 1144 |
| NsiI | ATGCAT | 1 cut(s) | 368 |
| NspI | RCATGY | 1 cut(s) | 370 |
| NspV | TTCGAA | 1 cut(s) | 1029 |
| PaeI | GCATGC | 1 cut(s) | 370 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 217, 1026 |
| PctI | GAATGC | 1 cut(s) | 1229 |
| PdmI | GAANNNNTTC | 1 cut(s) | 725 |
| PfeI | GAWTC | 5 cut(s) | 170, 242, 581, 868, 1254 |
| PfoI | TCCNGGA | 1 cut(s) | 838 |
| PkrI | GCNGC | 1 cut(s) | 1244 |
| PleI | GAGTC | 4 cut(s) | 655, 813, 974, 1018 |
| PpsI | GAGTC | 4 cut(s) | 655, 813, 974, 1018 |
| PpuMI | RGGWCCY | 1 cut(s) | 836 |
| PsiI | TTATAA | 1 cut(s) | 432 |
| Psp5II | RGGWCCY | 1 cut(s) | 836 |
| Psp6I | CCWGG | 4 cut(s) | 247, 282, 838, 1284 |
| PspEI | GGTNACC | 1 cut(s) | 847 |
| PspGI | CCWGG | 4 cut(s) | 247, 282, 838, 1284 |
| PspN4I | GGNNCC | 3 cut(s) | 744, 922, 1138 |
| PspPI | GGNCC | 2 cut(s) | 836, 1136 |
| PspPPI | RGGWCCY | 1 cut(s) | 836 |
| PstNI | CAGNNNCTG | 1 cut(s) | 839 |
| PsuI | RGATCY | 2 cut(s) | 1081, 1099 |
| RsaI | GTAC | 3 cut(s) | 281, 304, 1001 |
| RsaNI | GTAC | 3 cut(s) | 280, 303, 1000 |
| RseI | CAYNNNNRTG | 2 cut(s) | 624, 1158 |
| SalI | GTCGAC | 2 cut(s) | 332, 816 |
| SaqAI | TTAA | 3 cut(s) | 164, 960, 1158 |
| SatI | GCNGC | 1 cut(s) | 1243 |
| Sau3AI | GATC | 7 cut(s) | 120, 346, 409, 972, 1057, 1081, 1099 |
| Sau96I | GGNCC | 2 cut(s) | 836, 1136 |
| SchI | GAGTC | 4 cut(s) | 656, 813, 974, 1019 |
| ScrFI | CCNGG | 4 cut(s) | 249, 284, 840, 1286 |
| SduI | GDGCHC | 2 cut(s) | 256, 377 |
| SexAI | ACCWGGT | 3 cut(s) | 247, 282, 1284 |
| SfaNI | GCATC | 4 cut(s) | 145, 458, 931, 937 |
| SfcI | CTRYAG | 1 cut(s) | 265 |
| SfuI | TTCGAA | 1 cut(s) | 1029 |
| SinI | GGWCC | 1 cut(s) | 836 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 624, 1158 |
| SmlI | CTYRAG | 1 cut(s) | 896 |
| SmoI | CTYRAG | 1 cut(s) | 896 |
| SphI | GCATGC | 1 cut(s) | 370 |
| Sse9I | AATT | 7 cut(s) | 327, 496, 654, 1038, 1180, 1192, 1279 |
| SsiI | CCGC | 5 cut(s) | 99, 339, 615, 717, 1092 |
| SspI | AATATT | 1 cut(s) | 725 |
| SspMI | CTAG | 2 cut(s) | 665, 1301 |
| StyD4I | CCNGG | 4 cut(s) | 247, 282, 838, 1284 |
| StyI | CCWWGG | 1 cut(s) | 951 |
| TaaI | ACNGT | 7 cut(s) | 95, 128, 221, 571, 937, 1219, 1315 |
| TaiI | ACGT | 3 cut(s) | 208, 764, 1001 |
| TaqI | TCGA | 5 cut(s) | 333, 584, 817, 1029, 1060 |
| TasI | AATT | 7 cut(s) | 327, 496, 654, 1038, 1180, 1192, 1279 |
| TfiI | GAWTC | 5 cut(s) | 170, 242, 581, 868, 1254 |
| Tru1I | TTAA | 3 cut(s) | 164, 960, 1158 |
| Tru9I | TTAA | 3 cut(s) | 164, 960, 1158 |
| TscAI | CASTG | 4 cut(s) | 100, 277, 582, 643 |
| TseFI | GTSAC | 2 cut(s) | 128, 1144 |
| TseI | GCWGC | 1 cut(s) | 1242 |
| Tsp45I | GTSAC | 2 cut(s) | 128, 1144 |
| TspDTI | ATGAA | 5 cut(s) | 255, 310, 411, 513, 819 |
| TspGWI | ACGGA | 2 cut(s) | 392, 564 |
| TspRI | CASTG | 4 cut(s) | 100, 277, 582, 643 |
| VneI | GTGCAC | 1 cut(s) | 373 |
| VpaK11BI | GGWCC | 1 cut(s) | 836 |
| XapI | RAATTY | 1 cut(s) | 1180 |
| XceI | RCATGY | 1 cut(s) | 370 |
| XmiI | GTMKAC | 2 cut(s) | 333, 817 |
| XmnI | GAANNNNTTC | 1 cut(s) | 725 |
| XspI | CTAG | 2 cut(s) | 665, 1301 |
| Zsp2I | ATGCAT | 1 cut(s) | 368 |