Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 156, 470 |
| AciI | CCGC | 1 cut(s) | 461 |
| AcoI | YGGCCR | 1 cut(s) | 462 |
| AfaI | GTAC | 2 cut(s) | 513, 539 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 119, 410, 411 |
| AgsI | TTSAA | 5 cut(s) | 41, 52, 275, 367, 429 |
| AhlI | ACTAGT | 1 cut(s) | 175 |
| AjnI | CCWGG | 3 cut(s) | 118, 474, 519 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 359, 391 |
| AleI | CACNNNNGTG | 1 cut(s) | 32 |
| AluBI | AGCT | 5 cut(s) | 20, 46, 200, 258, 559 |
| AluI | AGCT | 5 cut(s) | 20, 46, 200, 258, 559 |
| Alw26I | GTCTC | 1 cut(s) | 220 |
| Ama87I | CYCGRG | 1 cut(s) | 201 |
| AoxI | GGCC | 3 cut(s) | 5, 291, 462 |
| ApeKI | GCWGC | 1 cut(s) | 255 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 66, 98 |
| AspS9I | GGNCC | 2 cut(s) | 104, 493 |
| AsuC2I | CCSGG | 1 cut(s) | 405 |
| AsuHPI | GGTGA | 2 cut(s) | 419, 509 |
| AvaI | CYCGRG | 1 cut(s) | 201 |
| AvaII | GGWCC | 2 cut(s) | 104, 493 |
| BbvI | GCAGC | 1 cut(s) | 267 |
| BccI | CCATC | 2 cut(s) | 404, 541 |
| BciT130I | CCWGG | 3 cut(s) | 120, 476, 521 |
| BcnI | CCSGG | 1 cut(s) | 405 |
| BcoDI | GTCTC | 1 cut(s) | 220 |
| BcuI | ACTAGT | 1 cut(s) | 175 |
| BfaI | CTAG | 1 cut(s) | 176 |
| BfmI | CTRYAG | 1 cut(s) | 30 |
| BisI | GCNGC | 2 cut(s) | 256, 462 |
| BlsI | GCNGC | 2 cut(s) | 257, 463 |
| BmcAI | AGTACT | 1 cut(s) | 539 |
| Bme1390I | CCNGG | 4 cut(s) | 120, 405, 476, 521 |
| Bme18I | GGWCC | 2 cut(s) | 104, 493 |
| BmeT110I | CYCGRG | 1 cut(s) | 201 |
| BmgT120I | GGNCC | 2 cut(s) | 104, 493 |
| BmiI | GGNNCC | 1 cut(s) | 495 |
| BmrFI | CCNGG | 4 cut(s) | 120, 405, 476, 521 |
| BpuEI | CTTGAG | 1 cut(s) | 562 |
| BpuMI | CCSGG | 1 cut(s) | 405 |
| BsaJI | CCNNGG | 1 cut(s) | 474 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 119, 410, 411 |
| BseBI | CCWGG | 3 cut(s) | 120, 476, 521 |
| BseDI | CCNNGG | 1 cut(s) | 474 |
| BseGI | GGATG | 3 cut(s) | 19, 552, 559 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 119, 410, 411 |
| BseXI | GCAGC | 1 cut(s) | 267 |
| BshFI | GGCC | 3 cut(s) | 7, 293, 464 |
| BsiHKCI | CYCGRG | 1 cut(s) | 201 |
| BsiSI | CCGG | 1 cut(s) | 405 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 119, 410, 411 |
| BsmAI | GTCTC | 1 cut(s) | 220 |
| BsnI | GGCC | 3 cut(s) | 7, 293, 464 |
| BsoBI | CYCGRG | 1 cut(s) | 201 |
| BspACI | CCGC | 1 cut(s) | 461 |
| BspANI | GGCC | 3 cut(s) | 7, 293, 464 |
| BspHI | TCATGA | 1 cut(s) | 97 |
| BspLI | GGNNCC | 1 cut(s) | 495 |
| BspMAI | CTGCAG | 1 cut(s) | 34 |
| BssECI | CCNNGG | 1 cut(s) | 474 |
| Bst2UI | CCWGG | 3 cut(s) | 120, 476, 521 |
| Bst4CI | ACNGT | 2 cut(s) | 251, 271 |
| BstC8I | GCNNGC | 1 cut(s) | 186 |
| BstF5I | GGATG | 3 cut(s) | 19, 552, 559 |
| BstMAI | GTCTC | 1 cut(s) | 220 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 299 |
| BstNI | CCWGG | 3 cut(s) | 120, 476, 521 |
| BstSCI | CCNGG | 4 cut(s) | 118, 403, 474, 519 |
| BstSFI | CTRYAG | 1 cut(s) | 30 |
| BstV1I | GCAGC | 1 cut(s) | 267 |
| BsuRI | GGCC | 3 cut(s) | 7, 293, 464 |
| BtsCI | GGATG | 3 cut(s) | 19, 552, 559 |
| BtsI | GCAGTG | 2 cut(s) | 27, 39 |
| BtsIMutI | CAGTG | 3 cut(s) | 27, 39, 172 |
| Cac8I | GCNNGC | 1 cut(s) | 186 |
| CciI | TCATGA | 1 cut(s) | 97 |
| Cfr13I | GGNCC | 2 cut(s) | 104, 493 |
| Csp6I | GTAC | 2 cut(s) | 512, 538 |
| CviAII | CATG | 2 cut(s) | 98, 359 |
| CviQI | GTAC | 2 cut(s) | 512, 538 |
| EaeI | YGGCCR | 1 cut(s) | 462 |
| Eco147I | AGGCCT | 1 cut(s) | 7 |
| Eco47I | GGWCC | 2 cut(s) | 104, 493 |
| Eco88I | CYCGRG | 1 cut(s) | 201 |
| EcoRII | CCWGG | 3 cut(s) | 118, 474, 519 |
| FaeI | CATG | 2 cut(s) | 101, 362 |
| FaiI | YATR | 6 cut(s) | 99, 197, 343, 349, 360, 401 |
| FatI | CATG | 2 cut(s) | 97, 358 |
| FblI | GTMKAC | 2 cut(s) | 156, 470 |
| Fnu4HI | GCNGC | 2 cut(s) | 256, 462 |
| FokI | GGATG | 2 cut(s) | 26, 559 |
| Fsp4HI | GCNGC | 2 cut(s) | 256, 462 |
| FspBI | CTAG | 1 cut(s) | 176 |
| GluI | GCNGC | 2 cut(s) | 256, 462 |
| HaeIII | GGCC | 3 cut(s) | 7, 293, 464 |
| HapII | CCGG | 1 cut(s) | 405 |
| Hin1II | CATG | 2 cut(s) | 101, 362 |
| HinfI | GANTC | 1 cut(s) | 170 |
| HpaII | CCGG | 1 cut(s) | 405 |
| HphI | GGTGA | 2 cut(s) | 419, 509 |
| Hpy166II | GTNNAC | 4 cut(s) | 57, 157, 243, 471 |
| Hpy188I | TCNGA | 2 cut(s) | 334, 436 |
| Hpy188III | TCNNGA | 3 cut(s) | 85, 98, 107 |
| Hpy8I | GTNNAC | 4 cut(s) | 57, 157, 243, 471 |
| HpyAV | CCTTC | 2 cut(s) | 95, 102 |
| HpyCH4III | ACNGT | 2 cut(s) | 251, 271 |
| HpyCH4IV | ACGT | 1 cut(s) | 162 |
| HpyCH4V | TGCA | 2 cut(s) | 32, 188 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 299 |
| HpySE526I | ACGT | 1 cut(s) | 162 |
| Hsp92II | CATG | 2 cut(s) | 101, 362 |
| Lsp1109I | GCAGC | 1 cut(s) | 267 |
| MaeI | CTAG | 1 cut(s) | 176 |
| MaeII | ACGT | 1 cut(s) | 162 |
| MaeIII | GTNAC | 1 cut(s) | 167 |
| MluCI | AATT | 2 cut(s) | 208, 504 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MmeI | TCCRAC | 1 cut(s) | 471 |
| MnlI | CCTC | 6 cut(s) | 80, 149, 304, 340, 537, 544 |
| MseI | TTAA | 1 cut(s) | 562 |
| MslI | CAYNNNNRTG | 1 cut(s) | 32 |
| MspI | CCGG | 1 cut(s) | 405 |
| MspR9I | CCNGG | 4 cut(s) | 120, 405, 476, 521 |
| MvaI | CCWGG | 3 cut(s) | 120, 476, 521 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 299 |
| NciI | CCSGG | 1 cut(s) | 405 |
| NlaIII | CATG | 2 cut(s) | 101, 362 |
| NlaIV | GGNNCC | 1 cut(s) | 495 |
| NmuCI | GTSAC | 1 cut(s) | 167 |
| OliI | CACNNNNGTG | 1 cut(s) | 32 |
| PagI | TCATGA | 1 cut(s) | 97 |
| PceI | AGGCCT | 1 cut(s) | 7 |
| PkrI | GCNGC | 2 cut(s) | 257, 463 |
| PleI | GAGTC | 1 cut(s) | 164 |
| PpsI | GAGTC | 1 cut(s) | 164 |
| Psp6I | CCWGG | 3 cut(s) | 118, 474, 519 |
| PspGI | CCWGG | 3 cut(s) | 118, 474, 519 |
| PspN4I | GGNNCC | 1 cut(s) | 495 |
| PspPI | GGNCC | 2 cut(s) | 104, 493 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 140, 172 |
| PstI | CTGCAG | 1 cut(s) | 34 |
| RsaI | GTAC | 2 cut(s) | 513, 539 |
| RsaNI | GTAC | 2 cut(s) | 512, 538 |
| RseI | CAYNNNNRTG | 1 cut(s) | 32 |
| SaqAI | TTAA | 1 cut(s) | 562 |
| SatI | GCNGC | 2 cut(s) | 256, 462 |
| Sau96I | GGNCC | 2 cut(s) | 104, 493 |
| ScaI | AGTACT | 1 cut(s) | 539 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 4 cut(s) | 120, 405, 476, 521 |
| SfcI | CTRYAG | 1 cut(s) | 30 |
| SinI | GGWCC | 2 cut(s) | 104, 493 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 32 |
| SmlI | CTYRAG | 1 cut(s) | 541 |
| SmoI | CTYRAG | 1 cut(s) | 541 |
| SpeI | ACTAGT | 1 cut(s) | 175 |
| Sse9I | AATT | 2 cut(s) | 208, 504 |
| SseBI | AGGCCT | 1 cut(s) | 7 |
| SsiI | CCGC | 1 cut(s) | 461 |
| SspI | AATATT | 1 cut(s) | 397 |
| SspMI | CTAG | 1 cut(s) | 176 |
| StuI | AGGCCT | 1 cut(s) | 7 |
| StyD4I | CCNGG | 4 cut(s) | 118, 403, 474, 519 |
| TaaI | ACNGT | 2 cut(s) | 251, 271 |
| TaiI | ACGT | 1 cut(s) | 165 |
| TaqI | TCGA | 1 cut(s) | 84 |
| TasI | AATT | 2 cut(s) | 208, 504 |
| TatI | WGTACW | 1 cut(s) | 537 |
| TauI | GCSGC | 1 cut(s) | 464 |
| Tru1I | TTAA | 1 cut(s) | 562 |
| Tru9I | TTAA | 1 cut(s) | 562 |
| TscAI | CASTG | 3 cut(s) | 34, 39, 172 |
| TseFI | GTSAC | 1 cut(s) | 167 |
| TseI | GCWGC | 1 cut(s) | 255 |
| Tsp45I | GTSAC | 1 cut(s) | 167 |
| TspDTI | ATGAA | 2 cut(s) | 30, 114 |
| TspGWI | ACGGA | 1 cut(s) | 366 |
| TspRI | CASTG | 3 cut(s) | 34, 39, 172 |
| VpaK11BI | GGWCC | 2 cut(s) | 104, 493 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 505, 527 |
| XmiI | GTMKAC | 2 cut(s) | 156, 470 |
| XspI | CTAG | 1 cut(s) | 176 |
| ZrmI | AGTACT | 1 cut(s) | 539 |