MD04G1225600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000230
Physical Location & Seq
Reverse (-)
30549080 .. 30552534
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1225600.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGGCATCCGCAGACACTAGAGGTCTTTCTCTTTTACACAATTATGGTGTAGTGGCTATGATTTTGATCATTTATCTAGTCATATGCCATAGTTCAGCTATAGAAGCGAGGAACTCTGATGTTAATGGTGGATTTAGTGTCAAACTTATTCGCCGAAATTCTTTAAATTCACCGCTTCACAATCATAATCATAAAATTTCTCGACGTCTTATGGAAGAAAGCACCCCACAATCGGAACTAAAGCGTGACAAGGAGGGCGGTGAGGGTGCACAACTTATGAAGCTCTCTCTTGGAACTCCACCCTATGAGATTTATGCAGTGGCGGATACAGGAAGTACGTTGTTGTGGACGCAATGTGAGCCGTGTCCGACTTGCTACAAGCAAAAAAATCCCAAGTTCGACCCGAAGAAGTCCTCATCGTATGCCACCCTTCCATGTACTGCACACGAATGTAGTTATGCTAACGACACTGGCTACACATCTTGCTCAAATGATGATAAAAAAATTTGCAACTACAATTACACATACATGGATGATTCCATAACGCAAGGGGTGATGTCGAAAGAGACGATCACGTTTGGATCCAGCTCCGGGAATCCGGTTTCATTCAAAGACGTTGTGATTGGTTGTGGGCACAACAACACCGGGGAAACTTTCGCCGAAAATGAAATGGGAATAGTAGGGCTTGGAGCCGGAAATTTATCTATTATTTCTCAACTTGCTCCTCACGTTGGAGGCAAGAAATTCTCACATTGTCTCGTACCCTTCGATCCGGATCATCCCAATGATGCAAGCATCATGAGTTTTGGGAAGGGGAGTGAGGTTTCGGGTGAAGGTGTGGTTTCGACGCCATTGATCACTAGAGCAGACAAAAACCAATATTTCGTCACCGTTGAGGGATTCAGCGTCGGAGAACAGTTTGTGCCATTTAACTCATCAGGGTCGATTTCAAAAGGGAACATGTATCTCGACTCGGGAACCCAAGTGACAATGCTGCCACAAGATTTTTACGGCAGATTGGTAGAACAAGTAAAGAAGGTGATGAATTTATCGCTAAAACCAGTCGAAGTACACGACTATTCTGGAACTGTGCTTTGCTACAACACCACGGAGAATCTTAAAGCACCATTATTGACGGTTCATTTCGACGGCGGTGCTAAATTGCAGTTGTCACCGGCACAAACTTTCTATCAAAACAAACAAGATAAATTACACTGCTTTGGAATGCTTAACACAAGTGATACAATAGATGAAGGAGTTGGTCTCTATGGAAGCTATGCTCAGTCAAATTTTTTGATTGGTTTTGATCTTGAAAAAATGTTGGTCTCCTTCAAGGCAGTTGACTGCAGAAAAACTAGCAGTCCTAGTCCTAGCCCTAGTCCTAGTCCTAGCCCTAGTCCTAGTCCTAATAGATTTCTATGGCCGATTGATAGAACAAGTAATGAAGGCAGTTGGTCTGAAACCGGAACATCTCCAAGGAGCGACATTGACGGTGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

501

Amino Acids

54.32

Weight (kDa)

5.82

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 92 - 271 7.3e-50 Xylanase inhibitor N-terminal
Asp PF00026 94 - 444 8.4e-12 Eukaryotic aspartyl protease
TAXi_C PF14541 293 - 445 2.7e-25 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02620 FvH4_6g02640 FvH4_6g02690 FvH4_6g03060 FvH4_6g03100 FvH4_6g03360 FvH4_6g03370 FvH4_6g18770 FvH4_6g24220 FvH4_6g49710
malus_domestica MD04G1225600.v1.1 MD04G1227300.v1.1 MD12G1242600.v1.1 MD12G1242700.v1.1 MD12G1242800.v1.1
prunus_persica Prupe.2G103000_v2.0.a1 Prupe.6G344200_v2.0.a1 Prupe.6G344300_v2.0.a1 Prupe.6G344400_v2.0.a1 Prupe.6G346300_v2.0.a1 Prupe.6G346400_v2.0.a1
pyrus_communis pycom12g22170
rosa_chinensis RchiOBHm_Chr3g0450531 RchiOBHm_Chr3g0450541 RchiOBHm_Chr3g0450561 RchiOBHm_Chr3g0450681 RchiOBHm_Chr3g0450691 RchiOBHm_Chr3g0450711 RchiOBHm_Chr3g0451881 RchiOBHm_Chr3g0454771 RchiOBHm_Chr3g0473771 RchiOBHm_Chr3g0473781 RchiOBHm_Chr3g0477881 RchiOBHm_Chr6g0253861
rosa_laevigata RLG00000014956 RLG00000023682 RLG00000023683 RLG00000023985 RLG00000025416 RLG00000025641 RLG00000025642 RLG00000025708 RLG00000025710 RLG00000025711 RLG00000025720 RLG00000025722 RLG00000025723 RLG00000025733 RLG00000035252
rosa_multiflora Rmu_sc0034928.1_g000007
rosa_roxburghii Rroxscaffold_164G00436250 Rroxscaffold_1G00021590 Rroxscaffold_1G00029330 Rroxscaffold_1G00029340 Rroxscaffold_6G00403630 Rroxscaffold_6G00407810 Rroxscaffold_6G00424780 Rroxscaffold_6G00428220 Rroxscaffold_6G00428250 Rroxscaffold_6G00428270 Rroxscaffold_6G00428340 Rroxscaffold_6G00428350 Rroxscaffold_6G00428380 Rroxscaffold_7G00211410
rosa_rugosa Rorug01G0107600 Rorug01G0107600 Rorug02G0450900 Rorug02G0627600 Rorug02G0628400 Rorug02G0628500 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628600 Rorug02G0628700 Rorug02G0629600 Rorug02G0629700 Rorug02G0629800 Rorug02G0636700 Rorug03G0002400 Rorug03G0135400 Rorug03G0135500 Rorug03G0163700 Rorug05G0548500
rosa_samantha Rh2DG537800 Rh3AG029200 Rh3AG029300 Rh3AG029500 Rh3AG030500 Rh3AG030700 Rh3AG038600 Rh3AG062400 Rh3AG214300 Rh3BG029600 Rh3BG029700 Rh3BG029900 Rh3BG030800 Rh3BG031000 Rh3BG031200 Rh3BG039900 Rh3BG064300 Rh3BG214100 Rh3BG214300 Rh3BG247800 Rh3CG028500 Rh3CG028600 Rh3CG028800 Rh3CG029600 Rh3CG029800 Rh3CG030000 Rh3CG038400 Rh3CG063200 Rh3CG210800 Rh3CG211000 Rh3CG241900 Rh3DG029300 Rh3DG029400 Rh3DG029600 Rh3DG030400 Rh3DG030600 Rh3DG030900 Rh3DG039200 Rh3DG064000 Rh3DG210000 Rh3DG210200 Rh3DG241200 Rh6AG064000 Rh6BG000600 Rh6BG057900 Rh6CG008800 Rh6CG057800 Rh6DG009600 Rh6DG054800
rosa_wichuraiana Rw2G042600 Rw3G002250 Rw3G002270 Rw3G002340 Rw3G002350 Rw3G002370 Rw3G002890 Rw3G004860 Rw3G016980 Rw3G016990 Rw6G005680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 208
AccIII TCCGGA 1 cut(s) 772
AciI CCGC 5 cut(s) 9, 173, 258, 323, 1152
AclWI GGATC 4 cut(s) 576, 589, 764, 783
AcoI YGGCCR 1 cut(s) 1421
AcsI RAATTY 8 cut(s) 157, 166, 195, 504, 697, 743, 1045, 1288
AcyI GRCGYC 2 cut(s) 205, 848
AfaI GTAC 4 cut(s) 337, 439, 762, 1071
AfiI CCNNNNNNNGG 3 cut(s) 232, 591, 731
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 4 cut(s) 610, 951, 1313, 1333
AluBI AGCT 4 cut(s) 98, 283, 588, 1275
AluI AGCT 4 cut(s) 98, 283, 588, 1275
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 4 cut(s) 560, 761, 1268, 1330
Alw44I GTGCAC 1 cut(s) 267
AlwI GGATC 4 cut(s) 576, 589, 764, 783
Ama87I CYCGRG 1 cut(s) 973
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 1 cut(s) 1421
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 994
ApoI RAATTY 8 cut(s) 157, 166, 195, 504, 697, 743, 1045, 1288
AsuC2I CCSGG 2 cut(s) 592, 646
AsuHPI GGTGA 7 cut(s) 162, 272, 565, 842, 880, 1051, 1164
AvaI CYCGRG 1 cut(s) 973
BaeGI GKGCMC 2 cut(s) 271, 636
BamHI GGATCC 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 981
BceAI ACGGC 3 cut(s) 346, 1027, 1165
BcgI CGANNNNNNTGC 2 cut(s) 1136, 1170
BciVI GTATCC 1 cut(s) 319
BclI TGATCA 2 cut(s) 66, 855
BcnI CCSGG 2 cut(s) 592, 646
BcoDI GTCTC 4 cut(s) 560, 761, 1268, 1330
BfmI CTRYAG 2 cut(s) 99, 1345
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 1 cut(s) 995
BlsI GCNGC 1 cut(s) 996
Bme1390I CCNGG 2 cut(s) 592, 646
BmeT110I CYCGRG 1 cut(s) 973
BmiI GGNNCC 3 cut(s) 583, 691, 979
BmrFI CCNGG 2 cut(s) 592, 646
BmsI GCATC 3 cut(s) 14, 778, 804
BplI GAGNNNNNCTC 2 cut(s) 1248, 1280
BpuMI CCSGG 2 cut(s) 592, 646
BsaBI GATNNNNATC 2 cut(s) 65, 774
BsaHI GRCGYC 2 cut(s) 205, 848
BsaI GGTCTC 2 cut(s) 1268, 1330
BsaJI CCNNGG 3 cut(s) 645, 1107, 1475
BsaWI WCCGGW 3 cut(s) 598, 772, 1463
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 591, 731
Bse118I RCCGGY 1 cut(s) 1174
Bse1I ACTGG 2 cut(s) 475, 1061
Bse3DI GCAATG 1 cut(s) 359
Bse8I GATNNNNATC 2 cut(s) 65, 774
BseAI TCCGGA 1 cut(s) 772
BseDI CCNNGG 3 cut(s) 645, 1107, 1475
BseGI GGATG 3 cut(s) 5, 538, 778
BseJI GATNNNNATC 2 cut(s) 65, 774
BseLI CCNNNNNNNGG 3 cut(s) 232, 591, 731
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 1295
BseNI ACTGG 2 cut(s) 475, 1061
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 2 cut(s) 271, 636
BseXI GCAGC 1 cut(s) 981
BsgI GTGCAG 1 cut(s) 426
BshFI GGCC 1 cut(s) 1423
BsiHKAI GWGCWC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 973
BsiSI CCGG 7 cut(s) 591, 599, 645, 693, 773, 1175, 1464
BslI CCNNNNNNNGG 3 cut(s) 232, 591, 731
BsmAI GTCTC 4 cut(s) 560, 761, 1268, 1330
BsmBI CGTCTC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 1230
BsnI GGCC 1 cut(s) 1423
Bso31I GGTCTC 2 cut(s) 1268, 1330
BsoBI CYCGRG 1 cut(s) 973
Bsp1286I GDGCHC 2 cut(s) 271, 636
Bsp13I TCCGGA 1 cut(s) 772
Bsp143I GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
BspACI CCGC 5 cut(s) 9, 173, 258, 323, 1152
BspANI GGCC 1 cut(s) 1423
BspCNI CTCAG 1 cut(s) 1294
BspEI TCCGGA 1 cut(s) 772
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 3 cut(s) 583, 691, 979
BspMAI CTGCAG 1 cut(s) 1349
BspPI GGATC 4 cut(s) 576, 589, 764, 783
BspTNI GGTCTC 2 cut(s) 1268, 1330
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 1174
BsrI ACTGG 2 cut(s) 475, 1061
BssAI RCCGGY 1 cut(s) 1174
BssECI CCNNGG 3 cut(s) 645, 1107, 1475
BssMI GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
BssNI GRCGYC 2 cut(s) 205, 848
BssT1I CCWWGG 1 cut(s) 1475
Bst4CI ACNGT 5 cut(s) 892, 918, 1090, 1138, 1493
BstACI GRCGYC 2 cut(s) 205, 848
BstC8I GCNNGC 1 cut(s) 793
BstDEI CTNAG 1 cut(s) 1281
BstDSI CCRYGG 1 cut(s) 1107
BstF5I GGATG 3 cut(s) 5, 538, 778
BstKTI GATC 7 cut(s) 69, 573, 584, 772, 778, 858, 1309
BstMAI GTCTC 4 cut(s) 560, 761, 1268, 1330
BstMBI GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
BstMWI GCNNNNNNNGC 2 cut(s) 104, 358
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 2 cut(s) 590, 644
BstSFI CTRYAG 2 cut(s) 99, 1345
BstSLI GKGCMC 2 cut(s) 271, 636
BstV1I GCAGC 1 cut(s) 981
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 1 cut(s) 1423
BtgI CCRYGG 1 cut(s) 1107
BtsCI GGATG 3 cut(s) 5, 538, 778
BtsI GCAGTG 2 cut(s) 324, 1213
BtsIMutI CAGTG 3 cut(s) 324, 468, 1213
Cac8I GCNNGC 1 cut(s) 793
CciI TCATGA 1 cut(s) 798
Cfr10I RCCGGY 1 cut(s) 1174
CseI GACGC 3 cut(s) 358, 856, 895
Csp6I GTAC 4 cut(s) 336, 438, 761, 1070
CviAII CATG 4 cut(s) 435, 529, 799, 961
CviQI GTAC 4 cut(s) 336, 438, 761, 1070
DdeI CTNAG 1 cut(s) 1281
DpnI GATC 7 cut(s) 68, 572, 583, 771, 777, 857, 1308
DpnII GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
DraI TTTAAA 1 cut(s) 165
EaeI YGGCCR 1 cut(s) 1421
EciI GGCGGA 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 1475
Eco31I GGTCTC 2 cut(s) 1268, 1330
Eco88I CYCGRG 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 1475
ErhI CCWWGG 1 cut(s) 1475
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 4 cut(s) 438, 532, 802, 964
FatI CATG 4 cut(s) 434, 528, 798, 960
FauNDI CATATG 1 cut(s) 83
FbaI TGATCA 2 cut(s) 66, 855
Fnu4HI GCNGC 1 cut(s) 995
FokI GGATG 2 cut(s) 545, 765
Fsp4HI GCNGC 1 cut(s) 995
GluI GCNGC 1 cut(s) 995
HaeIII GGCC 1 cut(s) 1423
HapII CCGG 7 cut(s) 591, 599, 645, 693, 773, 1175, 1464
HgaI GACGC 3 cut(s) 358, 856, 895
Hin1I GRCGYC 2 cut(s) 205, 848
Hin1II CATG 4 cut(s) 438, 532, 802, 964
HincII GTYRAC 1 cut(s) 1342
HindII GTYRAC 1 cut(s) 1342
HinfI GANTC 5 cut(s) 536, 595, 900, 971, 1114
HpaII CCGG 7 cut(s) 591, 599, 645, 693, 773, 1175, 1464
HphI GGTGA 7 cut(s) 162, 272, 565, 842, 880, 1051, 1164
Hpy166II GTNNAC 4 cut(s) 269, 348, 1072, 1342
Hpy188I TCNGA 5 cut(s) 118, 235, 369, 911, 1459
Hpy188III TCNNGA 8 cut(s) 201, 773, 799, 968, 975, 1083, 1310, 1500
Hpy8I GTNNAC 4 cut(s) 269, 348, 1072, 1342
Hpy99I CGWCG 4 cut(s) 207, 850, 911, 1151
HpyAV CCTTC 8 cut(s) 440, 775, 805, 827, 1030, 1247, 1339, 1439
HpyCH4III ACNGT 5 cut(s) 892, 918, 1090, 1138, 1493
HpyCH4IV ACGT 5 cut(s) 205, 338, 575, 615, 729
HpyCH4V TGCA 8 cut(s) 269, 317, 443, 510, 791, 1165, 1347, 1496
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 358
HpyF3I CTNAG 1 cut(s) 1281
HpySE526I ACGT 5 cut(s) 205, 338, 575, 615, 729
Hsp92I GRCGYC 2 cut(s) 205, 848
Hsp92II CATG 4 cut(s) 438, 532, 802, 964
Kpn2I TCCGGA 1 cut(s) 772
Ksp22I TGATCA 2 cut(s) 66, 855
Kzo9I GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
LmnI GCTCC 4 cut(s) 593, 689, 727, 1479
Lsp1109I GCAGC 1 cut(s) 981
LweI GCATC 3 cut(s) 14, 778, 804
MaeII ACGT 5 cut(s) 205, 338, 575, 615, 729
MaeIII GTNAC 4 cut(s) 245, 886, 985, 1170
MalI GATC 7 cut(s) 68, 572, 583, 771, 777, 857, 1308
MboI GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
MboII GAAGA 2 cut(s) 227, 418
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 2 cut(s) 271, 636
MlyI GAGTC 1 cut(s) 965
MmeI TCCRAC 3 cut(s) 392, 712, 889
MnlI CCTC 9 cut(s) 14, 102, 247, 256, 424, 728, 735, 814, 889
MroI TCCGGA 1 cut(s) 772
MseI TTAA 5 cut(s) 123, 164, 930, 1119, 1230
MslI CAYNNNNRTG 4 cut(s) 42, 448, 527, 783
MspI CCGG 7 cut(s) 591, 599, 645, 693, 773, 1175, 1464
MspR9I CCNGG 2 cut(s) 592, 646
Mva1269I GAATGC 1 cut(s) 1230
MwoI GCNNNNNNNGC 2 cut(s) 104, 358
NciI CCSGG 2 cut(s) 592, 646
NdeI CATATG 1 cut(s) 83
NdeII GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
NlaIII CATG 4 cut(s) 438, 532, 802, 964
NlaIV GGNNCC 3 cut(s) 583, 691, 979
NmuCI GTSAC 4 cut(s) 245, 886, 985, 1170
NspI RCATGY 1 cut(s) 964
PagI TCATGA 1 cut(s) 798
PciI ACATGT 1 cut(s) 960
PcsI WCGNNNNNNNCGW 3 cut(s) 566, 765, 1071
PctI GAATGC 1 cut(s) 1230
PfeI GAWTC 4 cut(s) 536, 595, 900, 1114
PfoI TCCNGGA 1 cut(s) 590
PkrI GCNGC 1 cut(s) 996
PleI GAGTC 1 cut(s) 965
PpsI GAGTC 1 cut(s) 965
PscI ACATGT 1 cut(s) 960
PspN4I GGNNCC 3 cut(s) 583, 691, 979
PstI CTGCAG 1 cut(s) 1349
PsuI RGATCY 1 cut(s) 581
RsaI GTAC 4 cut(s) 337, 439, 762, 1071
RsaNI GTAC 4 cut(s) 336, 438, 761, 1070
RseI CAYNNNNRTG 4 cut(s) 42, 448, 527, 783
SaqAI TTAA 5 cut(s) 123, 164, 930, 1119, 1230
SatI GCNGC 1 cut(s) 995
Sau3AI GATC 7 cut(s) 66, 570, 581, 769, 775, 855, 1306
SchI GAGTC 1 cut(s) 965
ScrFI CCNGG 2 cut(s) 592, 646
SduI GDGCHC 2 cut(s) 271, 636
SfaNI GCATC 3 cut(s) 14, 778, 804
SfcI CTRYAG 2 cut(s) 99, 1345
SmiMI CAYNNNNRTG 4 cut(s) 42, 448, 527, 783
SsiI CCGC 5 cut(s) 9, 173, 258, 323, 1152
SspI AATATT 1 cut(s) 881
StyD4I CCNGG 2 cut(s) 590, 644
StyI CCWWGG 1 cut(s) 1475
TaaI ACNGT 5 cut(s) 892, 918, 1090, 1138, 1493
TaiI ACGT 5 cut(s) 208, 341, 578, 618, 732
TaqI TCGA 9 cut(s) 202, 399, 560, 768, 845, 944, 969, 1065, 1146
TatI WGTACW 2 cut(s) 437, 1069
TfiI GAWTC 4 cut(s) 536, 595, 900, 1114
Tru1I TTAA 5 cut(s) 123, 164, 930, 1119, 1230
Tru9I TTAA 5 cut(s) 123, 164, 930, 1119, 1230
TscAI CASTG 3 cut(s) 324, 475, 1220
TseFI GTSAC 4 cut(s) 245, 886, 985, 1170
TseI GCWGC 1 cut(s) 994
Tsp45I GTSAC 4 cut(s) 245, 886, 985, 1170
TspDTI ATGAA 7 cut(s) 293, 594, 681, 1058, 1130, 1266, 1458
TspGWI ACGGA 1 cut(s) 1124
TspRI CASTG 3 cut(s) 324, 475, 1220
VneI GTGCAC 1 cut(s) 267
XapI RAATTY 8 cut(s) 157, 166, 195, 504, 697, 743, 1045, 1288
XceI RCATGY 1 cut(s) 964
ZraI GACGTC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.