Belongs to the peptidase A1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1212 |
| Acc16I | TGCGCA | 1 cut(s) | 1306 |
| AccI | GTMKAC | 1 cut(s) | 442 |
| AccII | CGCG | 1 cut(s) | 521 |
| AciI | CCGC | 3 cut(s) | 287, 519, 531 |
| AclWI | GGATC | 1 cut(s) | 749 |
| AcoI | YGGCCR | 1 cut(s) | 253 |
| AcsI | RAATTY | 4 cut(s) | 157, 374, 731, 1112 |
| AcuI | CTGAAG | 2 cut(s) | 516, 1297 |
| AfaI | GTAC | 4 cut(s) | 512, 816, 955, 1234 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 719 |
| AgsI | TTSAA | 8 cut(s) | 528, 767, 884, 1072, 1141, 1160, 1241, 1291 |
| AjiI | CACGTC | 1 cut(s) | 680 |
| AluBI | AGCT | 4 cut(s) | 44, 221, 932, 1006 |
| AluI | AGCT | 4 cut(s) | 44, 221, 932, 1006 |
| Alw21I | GWGCWC | 1 cut(s) | 229 |
| AlwI | GGATC | 1 cut(s) | 749 |
| Ama87I | CYCGRG | 1 cut(s) | 391 |
| AoxI | GGCC | 2 cut(s) | 253, 682 |
| ApeKI | GCWGC | 1 cut(s) | 321 |
| ApoI | RAATTY | 4 cut(s) | 157, 374, 731, 1112 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 226, 258 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 641 |
| AspLEI | GCGC | 3 cut(s) | 131, 1176, 1307 |
| AspS9I | GGNCC | 3 cut(s) | 209, 548, 683 |
| AsuHPI | GGTGA | 2 cut(s) | 153, 818 |
| AvaI | CYCGRG | 1 cut(s) | 391 |
| AvaII | GGWCC | 2 cut(s) | 209, 548 |
| BalI | TGGCCA | 1 cut(s) | 255 |
| Bbv12I | GWGCWC | 1 cut(s) | 229 |
| BbvI | GCAGC | 1 cut(s) | 333 |
| BccI | CCATC | 3 cut(s) | 141, 199, 466 |
| BceAI | ACGGC | 1 cut(s) | 1219 |
| BfaI | CTAG | 1 cut(s) | 1268 |
| BfmI | CTRYAG | 2 cut(s) | 439, 585 |
| BfoI | RGCGCY | 1 cut(s) | 132 |
| BglII | AGATCT | 1 cut(s) | 774 |
| BisI | GCNGC | 2 cut(s) | 322, 519 |
| BlsI | GCNGC | 2 cut(s) | 323, 520 |
| Bme18I | GGWCC | 2 cut(s) | 209, 548 |
| BmeT110I | CYCGRG | 1 cut(s) | 391 |
| BmgBI | CACGTC | 1 cut(s) | 680 |
| BmgT120I | GGNCC | 3 cut(s) | 209, 548, 683 |
| BmiI | GGNNCC | 3 cut(s) | 210, 685, 711 |
| BmrI | ACTGGG | 1 cut(s) | 921 |
| BmuI | ACTGGG | 1 cut(s) | 921 |
| BpmI | CTGGAG | 1 cut(s) | 243 |
| BsaJI | CCNNGG | 1 cut(s) | 646 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 719 |
| Bse1I | ACTGG | 1 cut(s) | 916 |
| Bse3DI | GCAATG | 4 cut(s) | 306, 347, 739, 917 |
| BseDI | CCNNGG | 1 cut(s) | 646 |
| BseGI | GGATG | 2 cut(s) | 447, 578 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BseMI | GCAATG | 4 cut(s) | 306, 347, 739, 917 |
| BseMII | CTCAG | 2 cut(s) | 963, 1016 |
| BseNI | ACTGG | 1 cut(s) | 916 |
| BseXI | GCAGC | 1 cut(s) | 333 |
| Bsh1236I | CGCG | 1 cut(s) | 521 |
| BshFI | GGCC | 2 cut(s) | 255, 684 |
| BsiHKAI | GWGCWC | 1 cut(s) | 229 |
| BsiHKCI | CYCGRG | 1 cut(s) | 391 |
| BslFI | GGGAC | 1 cut(s) | 928 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BsmFI | GGGAC | 1 cut(s) | 928 |
| BsnI | GGCC | 2 cut(s) | 255, 684 |
| BsoBI | CYCGRG | 1 cut(s) | 391 |
| Bsp1286I | GDGCHC | 1 cut(s) | 229 |
| Bsp143I | GATC | 3 cut(s) | 325, 754, 774 |
| BspACI | CCGC | 3 cut(s) | 287, 519, 531 |
| BspANI | GGCC | 2 cut(s) | 255, 684 |
| BspCNI | CTCAG | 2 cut(s) | 962, 1015 |
| BspFNI | CGCG | 1 cut(s) | 521 |
| BspLI | GGNNCC | 3 cut(s) | 210, 685, 711 |
| BspPI | GGATC | 1 cut(s) | 749 |
| BsrDI | GCAATG | 4 cut(s) | 306, 347, 739, 917 |
| BsrI | ACTGG | 1 cut(s) | 916 |
| BssECI | CCNNGG | 1 cut(s) | 646 |
| BssMI | GATC | 3 cut(s) | 325, 754, 774 |
| Bst4CI | ACNGT | 6 cut(s) | 201, 351, 440, 568, 1018, 1102 |
| Bst6I | CTCTTC | 1 cut(s) | 1242 |
| BstC8I | GCNNGC | 2 cut(s) | 433, 533 |
| BstDEI | CTNAG | 2 cut(s) | 949, 1002 |
| BstDSI | CCRYGG | 1 cut(s) | 646 |
| BstF5I | GGATG | 2 cut(s) | 447, 578 |
| BstFNI | CGCG | 1 cut(s) | 521 |
| BstH2I | RGCGCY | 1 cut(s) | 132 |
| BstHHI | GCGC | 3 cut(s) | 131, 1176, 1307 |
| BstKTI | GATC | 3 cut(s) | 328, 757, 777 |
| BstMBI | GATC | 3 cut(s) | 325, 754, 774 |
| BstNSI | RCATGY | 1 cut(s) | 940 |
| BstSFI | CTRYAG | 2 cut(s) | 439, 585 |
| BstUI | CGCG | 1 cut(s) | 521 |
| BstV1I | GCAGC | 1 cut(s) | 333 |
| BstX2I | RGATCY | 2 cut(s) | 754, 774 |
| BstYI | RGATCY | 2 cut(s) | 754, 774 |
| BsuRI | GGCC | 2 cut(s) | 255, 684 |
| BtgI | CCRYGG | 1 cut(s) | 646 |
| BtrI | CACGTC | 1 cut(s) | 680 |
| BtsCI | GGATG | 2 cut(s) | 447, 578 |
| BtsIMutI | CAGTG | 1 cut(s) | 1107 |
| Cac8I | GCNNGC | 2 cut(s) | 433, 533 |
| CfoI | GCGC | 3 cut(s) | 131, 1176, 1307 |
| Cfr13I | GGNCC | 3 cut(s) | 209, 548, 683 |
| CseI | GACGC | 2 cut(s) | 346, 1090 |
| Csp6I | GTAC | 4 cut(s) | 511, 815, 954, 1233 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 100, 135 |
| CviAII | CATG | 4 cut(s) | 423, 515, 937, 1036 |
| CviQI | GTAC | 4 cut(s) | 511, 815, 954, 1233 |
| DdeI | CTNAG | 2 cut(s) | 949, 1002 |
| DpnI | GATC | 3 cut(s) | 327, 756, 776 |
| DpnII | GATC | 3 cut(s) | 325, 754, 774 |
| EaeI | YGGCCR | 1 cut(s) | 253 |
| Eam1104I | CTCTTC | 1 cut(s) | 1242 |
| EarI | CTCTTC | 1 cut(s) | 1242 |
| EciI | GGCGGA | 1 cut(s) | 276 |
| Eco47I | GGWCC | 2 cut(s) | 209, 548 |
| Eco57I | CTGAAG | 2 cut(s) | 516, 1297 |
| Eco88I | CYCGRG | 1 cut(s) | 391 |
| EcoRI | GAATTC | 1 cut(s) | 157 |
| FaeI | CATG | 4 cut(s) | 426, 518, 940, 1039 |
| FaqI | GGGAC | 1 cut(s) | 928 |
| FatI | CATG | 4 cut(s) | 422, 514, 936, 1035 |
| FauI | CCCGC | 1 cut(s) | 524 |
| FblI | GTMKAC | 1 cut(s) | 442 |
| Fnu4HI | GCNGC | 2 cut(s) | 322, 519 |
| FokI | GGATG | 2 cut(s) | 434, 565 |
| Fsp4HI | GCNGC | 2 cut(s) | 322, 519 |
| FspBI | CTAG | 1 cut(s) | 1268 |
| FspI | TGCGCA | 1 cut(s) | 1306 |
| GlaI | GCGC | 3 cut(s) | 130, 1175, 1306 |
| GluI | GCNGC | 2 cut(s) | 322, 519 |
| GsuI | CTGGAG | 1 cut(s) | 243 |
| HaeII | RGCGCY | 1 cut(s) | 132 |
| HaeIII | GGCC | 2 cut(s) | 255, 684 |
| HgaI | GACGC | 2 cut(s) | 346, 1090 |
| HhaI | GCGC | 3 cut(s) | 131, 1176, 1307 |
| Hin1II | CATG | 4 cut(s) | 426, 518, 940, 1039 |
| Hin6I | GCGC | 3 cut(s) | 129, 1174, 1305 |
| HinP1I | GCGC | 3 cut(s) | 129, 1174, 1305 |
| HincII | GTYRAC | 1 cut(s) | 946 |
| HindII | GTYRAC | 1 cut(s) | 946 |
| HinfI | GANTC | 5 cut(s) | 242, 444, 454, 947, 1028 |
| HphI | GGTGA | 2 cut(s) | 153, 818 |
| Hpy166II | GTNNAC | 5 cut(s) | 443, 511, 946, 956, 971 |
| Hpy188I | TCNGA | 2 cut(s) | 143, 247 |
| Hpy188III | TCNNGA | 6 cut(s) | 212, 451, 494, 784, 870, 1252 |
| Hpy8I | GTNNAC | 5 cut(s) | 443, 511, 946, 956, 971 |
| Hpy99I | CGWCG | 2 cut(s) | 201, 1084 |
| HpyAV | CCTTC | 3 cut(s) | 20, 138, 803 |
| HpyCH4III | ACNGT | 6 cut(s) | 201, 351, 440, 568, 1018, 1102 |
| HpyCH4IV | ACGT | 2 cut(s) | 679, 967 |
| HpyCH4V | TGCA | 3 cut(s) | 922, 1039, 1135 |
| HpyF3I | CTNAG | 2 cut(s) | 949, 1002 |
| HpySE526I | ACGT | 2 cut(s) | 679, 967 |
| Hsp92II | CATG | 4 cut(s) | 426, 518, 940, 1039 |
| HspAI | GCGC | 3 cut(s) | 129, 1174, 1305 |
| Kzo9I | GATC | 3 cut(s) | 325, 754, 774 |
| LmnI | GCTCC | 2 cut(s) | 224, 715 |
| Lsp1109I | GCAGC | 1 cut(s) | 333 |
| MaeI | CTAG | 1 cut(s) | 1268 |
| MaeII | ACGT | 2 cut(s) | 679, 967 |
| MaeIII | GTNAC | 5 cut(s) | 77, 85, 568, 963, 1058 |
| MalI | GATC | 3 cut(s) | 327, 756, 776 |
| MboI | GATC | 3 cut(s) | 325, 754, 774 |
| MboII | GAAGA | 2 cut(s) | 509, 1229 |
| MflI | RGATCY | 2 cut(s) | 754, 774 |
| MhlI | GDGCHC | 1 cut(s) | 229 |
| MlsI | TGGCCA | 1 cut(s) | 255 |
| MluNI | TGGCCA | 1 cut(s) | 255 |
| MlyI | GAGTC | 3 cut(s) | 438, 941, 1037 |
| MmeI | TCCRAC | 1 cut(s) | 700 |
| MnlI | CCTC | 6 cut(s) | 412, 601, 716, 969, 1040, 1223 |
| Mox20I | TGGCCA | 1 cut(s) | 255 |
| MroXI | GAANNNNTTC | 1 cut(s) | 641 |
| MscI | TGGCCA | 1 cut(s) | 255 |
| MseI | TTAA | 3 cut(s) | 903, 1020, 1055 |
| Msp20I | TGGCCA | 1 cut(s) | 255 |
| MspA1I | CMGCKG | 1 cut(s) | 221 |
| MvnI | CGCG | 1 cut(s) | 521 |
| NdeII | GATC | 3 cut(s) | 325, 754, 774 |
| NlaIII | CATG | 4 cut(s) | 426, 518, 940, 1039 |
| NlaIV | GGNNCC | 3 cut(s) | 210, 685, 711 |
| NmuCI | GTSAC | 1 cut(s) | 963 |
| NsbI | TGCGCA | 1 cut(s) | 1306 |
| NspI | RCATGY | 1 cut(s) | 940 |
| PdmI | GAANNNNTTC | 1 cut(s) | 641 |
| PfeI | GAWTC | 2 cut(s) | 242, 454 |
| PkrI | GCNGC | 2 cut(s) | 323, 520 |
| PleI | GAGTC | 3 cut(s) | 438, 941, 1036 |
| PpsI | GAGTC | 3 cut(s) | 438, 941, 1036 |
| PsiI | TTATAA | 1 cut(s) | 1212 |
| PspN4I | GGNNCC | 3 cut(s) | 210, 685, 711 |
| PspPI | GGNCC | 3 cut(s) | 209, 548, 683 |
| PsuI | RGATCY | 2 cut(s) | 754, 774 |
| PvuII | CAGCTG | 1 cut(s) | 221 |
| RsaI | GTAC | 4 cut(s) | 512, 816, 955, 1234 |
| RsaNI | GTAC | 4 cut(s) | 511, 815, 954, 1233 |
| SaqAI | TTAA | 3 cut(s) | 903, 1020, 1055 |
| SatI | GCNGC | 2 cut(s) | 322, 519 |
| Sau3AI | GATC | 3 cut(s) | 325, 754, 774 |
| Sau96I | GGNCC | 3 cut(s) | 209, 548, 683 |
| SchI | GAGTC | 3 cut(s) | 438, 941, 1037 |
| SduI | GDGCHC | 1 cut(s) | 229 |
| SfcI | CTRYAG | 2 cut(s) | 439, 585 |
| SinI | GGWCC | 2 cut(s) | 209, 548 |
| SsiI | CCGC | 3 cut(s) | 287, 519, 531 |
| SspI | AATATT | 1 cut(s) | 763 |
| SspMI | CTAG | 1 cut(s) | 1268 |
| TaaI | ACNGT | 6 cut(s) | 201, 351, 440, 568, 1018, 1102 |
| TaiI | ACGT | 2 cut(s) | 682, 970 |
| TaqI | TCGA | 2 cut(s) | 196, 542 |
| TaqII | GACCGA | 2 cut(s) | 451, 1005 |
| TauI | GCSGC | 1 cut(s) | 521 |
| TfiI | GAWTC | 2 cut(s) | 242, 454 |
| Tru1I | TTAA | 3 cut(s) | 903, 1020, 1055 |
| Tru9I | TTAA | 3 cut(s) | 903, 1020, 1055 |
| TscAI | CASTG | 1 cut(s) | 1107 |
| TseFI | GTSAC | 1 cut(s) | 963 |
| TseI | GCWGC | 1 cut(s) | 321 |
| Tsp45I | GTSAC | 1 cut(s) | 963 |
| TspDTI | ATGAA | 5 cut(s) | 56, 281, 411, 1024, 1214 |
| TspGWI | ACGGA | 1 cut(s) | 663 |
| TspRI | CASTG | 1 cut(s) | 1107 |
| VpaK11BI | GGWCC | 2 cut(s) | 209, 548 |
| XapI | RAATTY | 4 cut(s) | 157, 374, 731, 1112 |
| XceI | RCATGY | 1 cut(s) | 940 |
| XmiI | GTMKAC | 1 cut(s) | 442 |
| XmnI | GAANNNNTTC | 1 cut(s) | 641 |
| XspI | CTAG | 1 cut(s) | 1268 |