Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 414 |
| AarI | CACCTGC | 1 cut(s) | 509 |
| Acc16I | TGCGCA | 2 cut(s) | 132, 627 |
| Acc36I | ACCTGC | 3 cut(s) | 495, 509, 1223 |
| AccI | GTMKAC | 1 cut(s) | 196 |
| AccII | CGCG | 1 cut(s) | 858 |
| AciI | CCGC | 1 cut(s) | 858 |
| AclWI | GGATC | 5 cut(s) | 236, 386, 482, 764, 959 |
| AcoI | YGGCCR | 1 cut(s) | 454 |
| AcsI | RAATTY | 4 cut(s) | 148, 447, 737, 1294 |
| AcuI | CTGAAG | 1 cut(s) | 109 |
| AdeI | CACNNNGTG | 1 cut(s) | 1003 |
| AfaI | GTAC | 1 cut(s) | 1108 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 725 |
| AgsI | TTSAA | 3 cut(s) | 944, 1147, 1339 |
| AluBI | AGCT | 1 cut(s) | 274 |
| AluI | AGCT | 1 cut(s) | 274 |
| Alw26I | GTCTC | 2 cut(s) | 365, 1336 |
| AlwI | GGATC | 5 cut(s) | 236, 386, 482, 764, 959 |
| AoxI | GGCC | 3 cut(s) | 291, 454, 1246 |
| ApeKI | GCWGC | 4 cut(s) | 5, 92, 95, 324 |
| ApoI | RAATTY | 4 cut(s) | 148, 447, 737, 1294 |
| AseI | ATTAAT | 1 cut(s) | 1140 |
| AspA2I | CCTAGG | 1 cut(s) | 758 |
| AspLEI | GCGC | 2 cut(s) | 133, 628 |
| AspS9I | GGNCC | 2 cut(s) | 209, 1246 |
| AsuC2I | CCSGG | 2 cut(s) | 290, 1001 |
| AsuHPI | GGTGA | 5 cut(s) | 449, 833, 989, 1082, 1268 |
| AsuII | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| AvaII | GGWCC | 1 cut(s) | 209 |
| AvrII | CCTAGG | 1 cut(s) | 758 |
| BbsI | GAAGAC | 1 cut(s) | 1028 |
| BbvI | GCAGC | 3 cut(s) | 104, 107, 336 |
| BccI | CCATC | 2 cut(s) | 251, 467 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 122, 156, 433, 467 |
| BciVI | GTATCC | 1 cut(s) | 757 |
| BcnI | CCSGG | 2 cut(s) | 290, 1001 |
| BcoDI | GTCTC | 2 cut(s) | 365, 1336 |
| BfaI | CTAG | 3 cut(s) | 39, 759, 1316 |
| BfmI | CTRYAG | 2 cut(s) | 502, 540 |
| BfuAI | ACCTGC | 3 cut(s) | 495, 509, 1223 |
| BfuI | GTATCC | 1 cut(s) | 757 |
| BisI | GCNGC | 4 cut(s) | 6, 93, 96, 325 |
| BlnI | CCTAGG | 1 cut(s) | 758 |
| BlsI | GCNGC | 4 cut(s) | 7, 94, 97, 326 |
| Bme1390I | CCNGG | 2 cut(s) | 290, 1001 |
| Bme18I | GGWCC | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 2 cut(s) | 209, 1246 |
| BmiI | GGNNCC | 1 cut(s) | 286 |
| BmrFI | CCNGG | 2 cut(s) | 290, 1001 |
| BmsI | GCATC | 3 cut(s) | 142, 853, 1107 |
| BpiI | GAAGAC | 1 cut(s) | 1028 |
| BpmI | CTGGAG | 1 cut(s) | 747 |
| Bpu14I | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| BpuEI | CTTGAG | 1 cut(s) | 969 |
| BpuMI | CCSGG | 2 cut(s) | 290, 1001 |
| BsaBI | GATNNNNATC | 2 cut(s) | 261, 768 |
| BsaI | GGTCTC | 1 cut(s) | 1336 |
| BsaJI | CCNNGG | 2 cut(s) | 288, 758 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 725 |
| Bse1I | ACTGG | 1 cut(s) | 457 |
| Bse3DI | GCAATG | 3 cut(s) | 164, 343, 842 |
| Bse8I | GATNNNNATC | 2 cut(s) | 261, 768 |
| BseDI | CCNNGG | 2 cut(s) | 288, 758 |
| BseGI | GGATG | 1 cut(s) | 868 |
| BseJI | GATNNNNATC | 2 cut(s) | 261, 768 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 725 |
| BseMI | GCAATG | 3 cut(s) | 164, 343, 842 |
| BseMII | CTCAG | 1 cut(s) | 1025 |
| BseNI | ACTGG | 1 cut(s) | 457 |
| BseRI | GAGGAG | 1 cut(s) | 699 |
| BseXI | GCAGC | 3 cut(s) | 104, 107, 336 |
| Bsh1236I | CGCG | 1 cut(s) | 858 |
| BshFI | GGCC | 3 cut(s) | 293, 456, 1248 |
| BsiSI | CCGG | 2 cut(s) | 290, 1000 |
| BslFI | GGGAC | 1 cut(s) | 943 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 725 |
| BsmAI | GTCTC | 2 cut(s) | 365, 1336 |
| BsmBI | CGTCTC | 1 cut(s) | 365 |
| BsmFI | GGGAC | 1 cut(s) | 943 |
| BsnI | GGCC | 3 cut(s) | 293, 456, 1248 |
| Bso31I | GGTCTC | 1 cut(s) | 1336 |
| Bsp119I | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| Bsp143I | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| BspACI | CCGC | 1 cut(s) | 858 |
| BspANI | GGCC | 3 cut(s) | 293, 456, 1248 |
| BspCNI | CTCAG | 1 cut(s) | 1024 |
| BspFNI | CGCG | 1 cut(s) | 858 |
| BspHI | TCATGA | 1 cut(s) | 1017 |
| BspLI | GGNNCC | 1 cut(s) | 286 |
| BspMAI | CTGCAG | 1 cut(s) | 506 |
| BspMI | ACCTGC | 3 cut(s) | 495, 509, 1223 |
| BspPI | GGATC | 5 cut(s) | 236, 386, 482, 764, 959 |
| BspT104I | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| BspTNI | GGTCTC | 1 cut(s) | 1336 |
| BsrDI | GCAATG | 3 cut(s) | 164, 343, 842 |
| BsrI | ACTGG | 1 cut(s) | 457 |
| BssECI | CCNNGG | 2 cut(s) | 288, 758 |
| BssMI | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| BssT1I | CCWWGG | 1 cut(s) | 758 |
| Bst4CI | ACNGT | 1 cut(s) | 565 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 50 |
| BstBI | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| BstC8I | GCNNGC | 1 cut(s) | 350 |
| BstDEI | CTNAG | 1 cut(s) | 1011 |
| BstEII | GGTNACC | 1 cut(s) | 1256 |
| BstF5I | GGATG | 1 cut(s) | 868 |
| BstFNI | CGCG | 1 cut(s) | 858 |
| BstHHI | GCGC | 2 cut(s) | 133, 628 |
| BstKTI | GATC | 7 cut(s) | 244, 394, 490, 772, 792, 876, 954 |
| BstMAI | GTCTC | 2 cut(s) | 365, 1336 |
| BstMBI | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 50, 722 |
| BstNSI | RCATGY | 1 cut(s) | 388 |
| BstPI | GGTNACC | 1 cut(s) | 1256 |
| BstSCI | CCNGG | 2 cut(s) | 288, 999 |
| BstSFI | CTRYAG | 2 cut(s) | 502, 540 |
| BstUI | CGCG | 1 cut(s) | 858 |
| BstV1I | GCAGC | 3 cut(s) | 104, 107, 336 |
| BstV2I | GAAGAC | 1 cut(s) | 1028 |
| BstX2I | RGATCY | 1 cut(s) | 487 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 191 |
| BstYI | RGATCY | 1 cut(s) | 487 |
| BsuI | GTATCC | 1 cut(s) | 757 |
| BsuRI | GGCC | 3 cut(s) | 293, 456, 1248 |
| BtsCI | GGATG | 1 cut(s) | 868 |
| BtsIMutI | CAGTG | 1 cut(s) | 133 |
| BveI | ACCTGC | 3 cut(s) | 495, 509, 1223 |
| Cac8I | GCNNGC | 1 cut(s) | 350 |
| CciI | TCATGA | 1 cut(s) | 1017 |
| CfoI | GCGC | 2 cut(s) | 133, 628 |
| Cfr13I | GGNCC | 2 cut(s) | 209, 1246 |
| Csp6I | GTAC | 1 cut(s) | 1107 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 1348 |
| CviAII | CATG | 4 cut(s) | 354, 380, 385, 1018 |
| CviQI | GTAC | 1 cut(s) | 1107 |
| DdeI | CTNAG | 1 cut(s) | 1011 |
| DpnI | GATC | 7 cut(s) | 243, 393, 489, 771, 791, 875, 953 |
| DpnII | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| DraI | TTTAAA | 2 cut(s) | 154, 1206 |
| DraIII | CACNNNGTG | 1 cut(s) | 1003 |
| EaeI | YGGCCR | 1 cut(s) | 454 |
| Eco130I | CCWWGG | 1 cut(s) | 758 |
| Eco31I | GGTCTC | 1 cut(s) | 1336 |
| Eco32I | GATATC | 1 cut(s) | 301 |
| Eco47I | GGWCC | 1 cut(s) | 209 |
| Eco57I | CTGAAG | 1 cut(s) | 109 |
| Eco91I | GGTNACC | 1 cut(s) | 1256 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1246 |
| EcoO65I | GGTNACC | 1 cut(s) | 1256 |
| EcoRV | GATATC | 1 cut(s) | 301 |
| EcoT14I | CCWWGG | 1 cut(s) | 758 |
| ErhI | CCWWGG | 1 cut(s) | 758 |
| Esp3I | CGTCTC | 1 cut(s) | 365 |
| FaeI | CATG | 4 cut(s) | 357, 383, 388, 1021 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 597, 629 |
| FaqI | GGGAC | 1 cut(s) | 943 |
| FatI | CATG | 4 cut(s) | 353, 379, 384, 1017 |
| FauI | CCCGC | 1 cut(s) | 851 |
| FblI | GTMKAC | 1 cut(s) | 196 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 93, 96, 325 |
| FokI | GGATG | 1 cut(s) | 875 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 93, 96, 325 |
| FspAI | RTGCGCAY | 1 cut(s) | 132 |
| FspBI | CTAG | 3 cut(s) | 39, 759, 1316 |
| FspI | TGCGCA | 2 cut(s) | 132, 627 |
| GlaI | GCGC | 2 cut(s) | 132, 627 |
| GluI | GCNGC | 4 cut(s) | 6, 93, 96, 325 |
| GsuI | CTGGAG | 1 cut(s) | 747 |
| HaeIII | GGCC | 3 cut(s) | 293, 456, 1248 |
| HapII | CCGG | 2 cut(s) | 290, 1000 |
| HhaI | GCGC | 2 cut(s) | 133, 628 |
| Hin1II | CATG | 4 cut(s) | 357, 383, 388, 1021 |
| Hin6I | GCGC | 2 cut(s) | 131, 626 |
| HinP1I | GCGC | 2 cut(s) | 131, 626 |
| HincII | GTYRAC | 1 cut(s) | 197 |
| HindII | GTYRAC | 1 cut(s) | 197 |
| HinfI | GANTC | 5 cut(s) | 851, 956, 1040, 1052, 1114 |
| HpaII | CCGG | 2 cut(s) | 290, 1000 |
| HphI | GGTGA | 5 cut(s) | 449, 833, 989, 1082, 1268 |
| Hpy166II | GTNNAC | 3 cut(s) | 197, 837, 1170 |
| Hpy188I | TCNGA | 4 cut(s) | 396, 800, 1066, 1290 |
| Hpy188III | TCNNGA | 5 cut(s) | 212, 245, 986, 1018, 1300 |
| Hpy8I | GTNNAC | 3 cut(s) | 197, 837, 1170 |
| Hpy99I | CGWCG | 2 cut(s) | 201, 1114 |
| HpyAV | CCTTC | 4 cut(s) | 304, 818, 878, 1345 |
| HpyCH4III | ACNGT | 1 cut(s) | 565 |
| HpyCH4IV | ACGT | 1 cut(s) | 199 |
| HpyCH4V | TGCA | 8 cut(s) | 169, 366, 444, 483, 504, 1098, 1165, 1353 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 50, 722 |
| HpyF3I | CTNAG | 1 cut(s) | 1011 |
| HpySE526I | ACGT | 1 cut(s) | 199 |
| Hsp92II | CATG | 4 cut(s) | 357, 383, 388, 1021 |
| HspAI | GCGC | 2 cut(s) | 131, 626 |
| Kzo9I | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| LmnI | GCTCC | 1 cut(s) | 721 |
| Lsp1109I | GCAGC | 3 cut(s) | 104, 107, 336 |
| LweI | GCATC | 3 cut(s) | 142, 853, 1107 |
| MaeI | CTAG | 3 cut(s) | 39, 759, 1316 |
| MaeII | ACGT | 1 cut(s) | 199 |
| MaeIII | GTNAC | 4 cut(s) | 565, 595, 1088, 1256 |
| MalI | GATC | 7 cut(s) | 243, 393, 489, 771, 791, 875, 953 |
| MboI | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| MboII | GAAGA | 1 cut(s) | 1033 |
| MfeI | CAATTG | 1 cut(s) | 312 |
| MflI | RGATCY | 1 cut(s) | 487 |
| MlyI | GAGTC | 1 cut(s) | 950 |
| MnlI | CCTC | 9 cut(s) | 415, 677, 680, 722, 942, 993, 1096, 1259, 1271 |
| MseI | TTAA | 7 cut(s) | 153, 572, 918, 1140, 1205, 1211, 1378 |
| MspI | CCGG | 2 cut(s) | 290, 1000 |
| MspR9I | CCNGG | 2 cut(s) | 290, 1001 |
| MunI | CAATTG | 1 cut(s) | 312 |
| MvnI | CGCG | 1 cut(s) | 858 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 50, 722 |
| NciI | CCSGG | 2 cut(s) | 290, 1001 |
| NdeII | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| NlaIII | CATG | 4 cut(s) | 357, 383, 388, 1021 |
| NlaIV | GGNNCC | 1 cut(s) | 286 |
| NmeAIII | GCCGAG | 1 cut(s) | 432 |
| NmuCI | GTSAC | 2 cut(s) | 1088, 1256 |
| NsbI | TGCGCA | 2 cut(s) | 132, 627 |
| NspI | RCATGY | 1 cut(s) | 388 |
| NspV | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| PagI | TCATGA | 1 cut(s) | 1017 |
| PaqCI | CACCTGC | 1 cut(s) | 509 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 459 |
| PfeI | GAWTC | 4 cut(s) | 851, 1040, 1052, 1114 |
| PkrI | GCNGC | 4 cut(s) | 7, 94, 97, 326 |
| PleI | GAGTC | 1 cut(s) | 950 |
| PpsI | GAGTC | 1 cut(s) | 950 |
| PshBI | ATTAAT | 1 cut(s) | 1140 |
| PsiI | TTATAA | 1 cut(s) | 414 |
| PspEI | GGTNACC | 1 cut(s) | 1256 |
| PspN4I | GGNNCC | 1 cut(s) | 286 |
| PspPI | GGNCC | 2 cut(s) | 209, 1246 |
| PstI | CTGCAG | 1 cut(s) | 506 |
| PsuI | RGATCY | 1 cut(s) | 487 |
| RsaI | GTAC | 1 cut(s) | 1108 |
| RsaNI | GTAC | 1 cut(s) | 1107 |
| SalI | GTCGAC | 1 cut(s) | 195 |
| SaqAI | TTAA | 7 cut(s) | 153, 572, 918, 1140, 1205, 1211, 1378 |
| SatI | GCNGC | 4 cut(s) | 6, 93, 96, 325 |
| Sau3AI | GATC | 7 cut(s) | 241, 391, 487, 769, 789, 873, 951 |
| Sau96I | GGNCC | 2 cut(s) | 209, 1246 |
| SbfI | CCTGCAGG | 1 cut(s) | 506 |
| SchI | GAGTC | 1 cut(s) | 950 |
| ScrFI | CCNGG | 2 cut(s) | 290, 1001 |
| SdaI | CCTGCAGG | 1 cut(s) | 506 |
| SfaNI | GCATC | 3 cut(s) | 142, 853, 1107 |
| SfcI | CTRYAG | 2 cut(s) | 502, 540 |
| SfuI | TTCGAA | 3 cut(s) | 142, 462, 1043 |
| SinI | GGWCC | 1 cut(s) | 209 |
| SmlI | CTYRAG | 1 cut(s) | 984 |
| SmoI | CTYRAG | 1 cut(s) | 984 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 506 |
| SsiI | CCGC | 1 cut(s) | 858 |
| SspI | AATATT | 2 cut(s) | 1185, 1285 |
| SspMI | CTAG | 3 cut(s) | 39, 759, 1316 |
| StyD4I | CCNGG | 2 cut(s) | 288, 999 |
| StyI | CCWWGG | 1 cut(s) | 758 |
| TaaI | ACNGT | 1 cut(s) | 565 |
| TaiI | ACGT | 1 cut(s) | 202 |
| TfiI | GAWTC | 4 cut(s) | 851, 1040, 1052, 1114 |
| Tru1I | TTAA | 7 cut(s) | 153, 572, 918, 1140, 1205, 1211, 1378 |
| Tru9I | TTAA | 7 cut(s) | 153, 572, 918, 1140, 1205, 1211, 1378 |
| TscAI | CASTG | 1 cut(s) | 133 |
| TseFI | GTSAC | 2 cut(s) | 1088, 1256 |
| TseI | GCWGC | 4 cut(s) | 5, 92, 95, 324 |
| Tsp45I | GTSAC | 2 cut(s) | 1088, 1256 |
| TspDTI | ATGAA | 7 cut(s) | 56, 284, 675, 1006, 1034, 1048, 1098 |
| TspGWI | ACGGA | 3 cut(s) | 268, 668, 903 |
| TspRI | CASTG | 1 cut(s) | 133 |
| VpaK11BI | GGWCC | 1 cut(s) | 209 |
| VspI | ATTAAT | 1 cut(s) | 1140 |
| XapI | RAATTY | 4 cut(s) | 148, 447, 737, 1294 |
| XceI | RCATGY | 1 cut(s) | 388 |
| XmaJI | CCTAGG | 1 cut(s) | 758 |
| XmiI | GTMKAC | 1 cut(s) | 196 |
| XspI | CTAG | 3 cut(s) | 39, 759, 1316 |