MD04G1226800.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005029
Physical Location & Seq
Reverse (-)
30622406 .. 30626136
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1226800.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAGAGGAAGGTGGTGGAGAAGAAGATTAATGGTGTCGTGGTGACTTCAATTGGTTTTTCTCGTGGTAAGGTCCACGTTGCTCCCAAAGTTTCAGAAGAAAGCATTGAATCAAAGGATTTTAAGGTGAAGAACCCTGTTGAAGAAAACAAAGTTGCTGTGGAATCTGAGGAGAAGCAAAATGTGCTGGCTGTTAAGAGTACAAATCTTGATGATGACTCGACTGATGCCAAAAATGAGAAACCTGAATTTCCGAAGCCGAGTGACATTAAAAAGTTGAACTTAAAATCCACTGCTGCTGAAAATGGGCATTCAAATCAGACTGTTGGGGCTGAAACTGCCACTGCCAAAAAATCCAGCAATTCTGAGTCTCCTGATGCTAATAAGAGCTCACAGTCTCCTCTTGCTACTAAGAGTCCACAGTCTCCCAATGCAGAGCCTCCCAAGAGTTCACAGCCAACTTCACCTCAGTCATCAGGAGAGGGCGCGGAACATGATAGTAAGAAGCATGCTAATGAAGAAGATATTTGGTCTCTGACTTCCTCAACTGCTGCATCAAGAACACCTAAATTCAAGGTTACAGTTGGACAAGCTCCCACGTTTAGGATCTACGAACGTATAGAGAAAAGGAAAGAGTTCTACTCGAAGTTGGAGGAAAAGCATCAAGCTATGGAGGCAGAGAGAAGCCAGTGGGAAGCAAGGGCCAAGGAAGAGCAAGAAGCAGCCATCAAGGCACTGAGGAAGAGCATGGTCTTCAAAGCAAATCCAATACCTAGTTTCATTTACGAGCCCCCTCCTCCTAAGGCTGAACGCAAGAAGCTTCCACTCACTCGGCCCAAGTCACCAAAACTAAACAGTCTAAGCCGGAGGAAGAGCTATGGCGATGCAATCACCAACTCATCCGCAGAGGAAAAAAAGAAAGGCTGTTCTCGGGCACAACGTCACAGCCTTGGCACTCATAAGGAAGAATCCACGCCTCCCAAAAGTAAGGCTCAGTCCGGAAGGCGAAACAGCCTTGGCGGTTGCAAGACCAAAGACCATTCGAAACACGAAAGGGAAACAACGGAAACATCTCGAAAGACCGCCGAGCAGACATCGGATTCGAATGACGATGGACACCAAAGCTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

378

Amino Acids

41.65

Weight (kDa)

9.43

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 200 - 272 2.3e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 488
AccIII TCCGGA 1 cut(s) 998
AciI CCGC 4 cut(s) 488, 903, 1020, 1083
AclWI GGATC 1 cut(s) 614
AcsI RAATTY 2 cut(s) 248, 569
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 603
AgsI TTSAA 7 cut(s) 51, 110, 143, 280, 315, 574, 757
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 6 cut(s) 390, 593, 668, 820, 876, 1125
AluI AGCT 6 cut(s) 390, 593, 668, 820, 876, 1125
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 4 cut(s) 375, 402, 429, 537
AlwI GGATC 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 930
Aor13HI TCCGGA 1 cut(s) 998
AoxI GGCC 2 cut(s) 702, 833
ApeKI GCWGC 3 cut(s) 296, 551, 722
ApoI RAATTY 2 cut(s) 248, 569
ArsI GACNNNNNNTTYG 2 cut(s) 1084, 1116
AseI ATTAAT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 3 cut(s) 73, 702, 834
AsuHPI GGTGA 5 cut(s) 55, 139, 456, 834, 883
AsuII TTCGAA 2 cut(s) 1043, 1103
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 1 cut(s) 73
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 1 cut(s) 937
BanII GRGCYC 2 cut(s) 392, 792
BauI CACGAG 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 745
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 3 cut(s) 283, 538, 734
BccI CCATC 2 cut(s) 734, 1106
BcoDI GTCTC 4 cut(s) 375, 402, 429, 537
BfaI CTAG 1 cut(s) 774
BglI GCCNNNNNGGC 1 cut(s) 731
BisI GCNGC 3 cut(s) 297, 552, 723
BlsI GCNGC 3 cut(s) 298, 553, 724
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 930
BmgT120I GGNCC 3 cut(s) 73, 702, 834
BmsI GCATC 5 cut(s) 217, 367, 563, 670, 874
BpiI GAAGAC 1 cut(s) 745
BplI GAGNNNNNCTC 2 cut(s) 626, 658
Bpu14I TTCGAA 2 cut(s) 1043, 1103
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 3 cut(s) 705, 949, 1015
BsaWI WCCGGW 1 cut(s) 998
Bsc4I CCNNNNNNNGG 1 cut(s) 603
Bse1I ACTGG 1 cut(s) 688
Bse21I CCTNAGG 1 cut(s) 801
BseAI TCCGGA 1 cut(s) 998
BseDI CCNNGG 3 cut(s) 705, 949, 1015
BseGI GGATG 1 cut(s) 899
BseLI CCNNNNNNNGG 1 cut(s) 603
BseMII CTCAG 5 cut(s) 159, 357, 482, 728, 1007
BseNI ACTGG 1 cut(s) 688
BseRI GAGGAG 3 cut(s) 185, 390, 786
BseSI GKGCMC 1 cut(s) 937
BseXI GCAGC 3 cut(s) 283, 538, 734
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 2 cut(s) 704, 835
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 2 cut(s) 865, 999
BslI CCNNNNNNNGG 1 cut(s) 603
BsmAI GTCTC 4 cut(s) 375, 402, 429, 537
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 2 cut(s) 704, 835
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 930
Bsp119I TTCGAA 2 cut(s) 1043, 1103
Bsp1286I GDGCHC 3 cut(s) 392, 792, 937
Bsp13I TCCGGA 1 cut(s) 998
Bsp143I GATC 1 cut(s) 606
BspACI CCGC 4 cut(s) 488, 903, 1020, 1083
BspANI GGCC 2 cut(s) 704, 835
BspCNI CTCAG 5 cut(s) 160, 358, 481, 729, 1006
BspEI TCCGGA 1 cut(s) 998
BspFNI CGCG 1 cut(s) 488
BspPI GGATC 1 cut(s) 614
BspQI GCTCTTC 3 cut(s) 705, 737, 866
BspT104I TTCGAA 2 cut(s) 1043, 1103
BspTNI GGTCTC 1 cut(s) 537
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 705, 949, 1015
BssMI GATC 1 cut(s) 606
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 3 cut(s) 705, 949, 1015
Bst2BI CACGAG 1 cut(s) 63
Bst4CI ACNGT 5 cut(s) 325, 396, 423, 583, 857
Bst6I CTCTTC 3 cut(s) 705, 737, 866
BstAPI GCANNNNNTGC 1 cut(s) 184
BstBI TTCGAA 2 cut(s) 1043, 1103
BstC8I GCNNGC 2 cut(s) 189, 510
BstDEI CTNAG 8 cut(s) 168, 366, 411, 468, 737, 801, 860, 993
BstF5I GGATG 1 cut(s) 899
BstFNI CGCG 1 cut(s) 488
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 1 cut(s) 609
BstMAI GTCTC 4 cut(s) 375, 402, 429, 537
BstMBI GATC 1 cut(s) 606
BstMWI GCNNNNNNNGC 4 cut(s) 184, 338, 674, 731
BstNSI RCATGY 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 937
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 283, 538, 734
BstV2I GAAGAC 1 cut(s) 745
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
Bsu36I CCTNAGG 1 cut(s) 801
BsuRI GGCC 2 cut(s) 704, 835
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 1 cut(s) 899
BtsI GCAGTG 2 cut(s) 291, 342
BtsIMutI CAGTG 4 cut(s) 291, 342, 695, 734
Cac8I GCNNGC 2 cut(s) 189, 510
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 3 cut(s) 73, 702, 834
Csp6I GTAC 1 cut(s) 201
CviAII CATG 3 cut(s) 494, 509, 748
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 8 cut(s) 168, 366, 411, 468, 737, 801, 860, 993
DpnI GATC 1 cut(s) 608
DpnII GATC 1 cut(s) 606
Eam1104I CTCTTC 3 cut(s) 705, 737, 866
EarI CTCTTC 3 cut(s) 705, 737, 866
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 3 cut(s) 705, 949, 1015
Eco24I GRGCYC 2 cut(s) 392, 792
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 930
EcoICRI GAGCTC 1 cut(s) 390
EcoT14I CCWWGG 3 cut(s) 705, 949, 1015
EcoT38I GRGCYC 2 cut(s) 392, 792
ErhI CCWWGG 3 cut(s) 705, 949, 1015
FaeI CATG 3 cut(s) 497, 512, 751
FaiI YATR 7 cut(s) 495, 510, 620, 671, 749, 879, 960
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FatI CATG 3 cut(s) 493, 508, 747
Fnu4HI GCNGC 3 cut(s) 297, 552, 723
FokI GGATG 1 cut(s) 886
FriOI GRGCYC 2 cut(s) 392, 792
Fsp4HI GCNGC 3 cut(s) 297, 552, 723
FspBI CTAG 1 cut(s) 774
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 297, 552, 723
HaeIII GGCC 2 cut(s) 704, 835
HapII CCGG 2 cut(s) 865, 999
HhaI GCGC 1 cut(s) 488
Hin1II CATG 3 cut(s) 497, 512, 751
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HindIII AAGCTT 2 cut(s) 818, 1123
HinfI GANTC 7 cut(s) 110, 164, 218, 368, 415, 968, 1100
HpaII CCGG 2 cut(s) 865, 999
HphI GGTGA 5 cut(s) 55, 139, 456, 834, 883
Hpy166II GTNNAC 3 cut(s) 76, 419, 452
Hpy188I TCNGA 7 cut(s) 97, 169, 255, 321, 367, 537, 1099
Hpy188III TCNNGA 6 cut(s) 209, 374, 477, 558, 999, 1074
Hpy8I GTNNAC 3 cut(s) 76, 419, 452
HpyAV CCTTC 2 cut(s) 4, 996
HpyCH4III ACNGT 5 cut(s) 325, 396, 423, 583, 857
HpyCH4IV ACGT 4 cut(s) 78, 599, 616, 940
HpyCH4V TGCA 4 cut(s) 434, 554, 887, 1026
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 338, 674, 731
HpyF3I CTNAG 8 cut(s) 168, 366, 411, 468, 737, 801, 860, 993
HpySE526I ACGT 4 cut(s) 78, 599, 616, 940
Hsp92II CATG 3 cut(s) 497, 512, 751
HspAI GCGC 1 cut(s) 486
Kpn2I TCCGGA 1 cut(s) 998
Kzo9I GATC 1 cut(s) 606
LguI GCTCTTC 3 cut(s) 705, 737, 866
LmnI GCTCC 2 cut(s) 88, 598
LpnPI CCDG 9 cut(s) 150, 173, 258, 370, 387, 462, 701, 878, 1012
Lsp1109I GCAGC 3 cut(s) 283, 538, 734
LweI GCATC 5 cut(s) 217, 367, 563, 670, 874
MaeI CTAG 1 cut(s) 774
MaeII ACGT 4 cut(s) 78, 599, 616, 940
MaeIII GTNAC 5 cut(s) 43, 263, 577, 840, 941
MalI GATC 1 cut(s) 608
MboI GATC 1 cut(s) 606
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 1 cut(s) 606
MhlI GDGCHC 3 cut(s) 392, 792, 937
MluCI AATT 4 cut(s) 51, 248, 361, 569
MlyI GAGTC 3 cut(s) 212, 377, 424
MmeI TCCRAC 2 cut(s) 565, 630
MroI TCCGGA 1 cut(s) 998
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 6 cut(s) 30, 123, 195, 270, 284, 1132
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 2 cut(s) 865, 999
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 310
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 184, 338, 674, 731
NdeII GATC 1 cut(s) 606
NlaIII CATG 3 cut(s) 497, 512, 751
NmeAIII GCCGAG 3 cut(s) 285, 811, 1111
NmuCI GTSAC 4 cut(s) 43, 263, 840, 941
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 2 cut(s) 1043, 1103
PaeI GCATGC 1 cut(s) 512
PciSI GCTCTTC 3 cut(s) 705, 737, 866
PcsI WCGNNNNNNNCGW 1 cut(s) 937
PctI GAATGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 4 cut(s) 110, 164, 968, 1100
PkrI GCNGC 3 cut(s) 298, 553, 724
PleI GAGTC 3 cut(s) 212, 376, 423
PpsI GAGTC 3 cut(s) 212, 376, 423
PshBI ATTAAT 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 392
PspPI GGNCC 3 cut(s) 73, 702, 834
PsuI RGATCY 1 cut(s) 606
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 513
SacI GAGCTC 1 cut(s) 392
SapI GCTCTTC 3 cut(s) 705, 737, 866
SaqAI TTAA 6 cut(s) 30, 123, 195, 270, 284, 1132
SatI GCNGC 3 cut(s) 297, 552, 723
Sau3AI GATC 1 cut(s) 606
Sau96I GGNCC 3 cut(s) 73, 702, 834
SchI GAGTC 3 cut(s) 212, 377, 424
SduI GDGCHC 3 cut(s) 392, 792, 937
SfaNI GCATC 5 cut(s) 217, 367, 563, 670, 874
SfuI TTCGAA 2 cut(s) 1043, 1103
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 513
SphI GCATGC 1 cut(s) 512
Sse9I AATT 4 cut(s) 51, 248, 361, 569
SsiI CCGC 4 cut(s) 488, 903, 1020, 1083
SspMI CTAG 1 cut(s) 774
SstI GAGCTC 1 cut(s) 392
StyI CCWWGG 3 cut(s) 705, 949, 1015
TaaI ACNGT 5 cut(s) 325, 396, 423, 583, 857
TaiI ACGT 4 cut(s) 81, 602, 619, 943
TaqI TCGA 5 cut(s) 221, 644, 1043, 1075, 1103
TasI AATT 4 cut(s) 51, 248, 361, 569
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 4 cut(s) 110, 164, 968, 1100
Tru1I TTAA 6 cut(s) 30, 123, 195, 270, 284, 1132
Tru9I TTAA 6 cut(s) 30, 123, 195, 270, 284, 1132
TscAI CASTG 4 cut(s) 298, 349, 695, 741
TseFI GTSAC 4 cut(s) 43, 263, 840, 941
TseI GCWGC 3 cut(s) 296, 551, 722
Tsp45I GTSAC 4 cut(s) 43, 263, 840, 941
TspDTI ATGAA 2 cut(s) 531, 769
TspGWI ACGGA 1 cut(s) 1079
TspRI CASTG 4 cut(s) 298, 349, 695, 741
VpaK11BI GGWCC 1 cut(s) 73
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 2 cut(s) 248, 569
XceI RCATGY 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.