RLG00000025725

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005029
Physical Location & Seq
Reverse (-)
47895081 .. 47901388
6308 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025725

Sequence Viewer

Length: 1254 bp
ATGCAGATACGTACGTACAGTACAGAGGCCATTACAACTGCACCAGCTAGCAGGCTCCCAGCTGATGATCGATCGGTTTCAGAAGATTCATGTGAGGTATATGAAGGTAGCTGCAGAAACCTTGACATGGGGAGGGAAATTACTGGTGTGAAGGAAGAGAAGAAAATTAATGGTGGTGTAGTGGGTACCAATGGTTTTTCTACTGCTAAAATCCATGTCTCTCCCAAAATTTCTGAGAGAATCAGCAGGATCGAGTCCAAGGAGTACAAGGTGAAGGACTCTAATGAAGAGAAAGCAGCTGAGGGGGAAGGTCTTGAGAAGCAAGATATGCTGGCTGTTAAGAGCACGAATCTTGATGATGACTTGACCAAGGAGAAAAATGAGAAACCTGGAACTCAGCAGTCAAGTGAGAGTTTAAATTCCAGTTCACCAGTTTCAAAATCTGCTGCTGTTGAAAATGGGCATTCAAATCAGACAGTTCCTCATCCCAATGCTCTGGTAACTGAAAGGGCTGGTACATGTGAAGATACTACTGGGGCTGAAGTTGCCACTTCAGAAAACTCCAGCACTGCAGAGTCTCCTAATGCAACTAAGACTTCACAGACTGCCAATGCAGAGTCACCTACTAAGAGTTCAGAGCCAAATTCACCTCGGTCATCACGACCTGATACTAAGAAGCATGCTGAGGAAGAAGATAATTGGTCTCTCACTTCCTCAACTGCTGCATCTGTGAGAACAAATAAATTCAAGGTTACAGTTGGACAAGCTCCTTCGTTTAAATGCAGTGAACGTGCACAAAAAAGAAATGAGTACTACTCAAAGTTAAAGGAAAAACAACAAGCTCTGGAGGCAGAGAGATTGCAGTACGAATCAAGGACCAGGGAAGAGCAAGAAGCAGCCATCAAGCAGTTGAGGAAGTCCTTGCTTCCACTTACGCGGCCCAAGTCACCAAAACTGAACAGTCTAAGCAGGAGAAAGAGCTTTGGTGATGCTCTCAACACATCTCATGACGAAAAGGGAATAGTTTGTCCTCGAGCACATCGTCACAGCGTTGGTTGCCAGAAAGAATGCACTGCATCGACTACTCCAAAGAATAAGGTGCAGAACAGTAGACGTAACAGTCTTGGCAATGGTAGCAAAGTCAAAGACCAATCAAAACAGGGAGAAGAAGAAACAACGAAAACAGCTCCTAAGTTCACGCAGAAGAGAAACGTAGACATCAGTTTGAATCATGAACTCGAAAGCTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

418

Amino Acids

45.83

Weight (kDa)

8.63

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 258 - 314 1.5e-15 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1119
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 2 cut(s) 1111, 1215
AccII CGCG 1 cut(s) 937
AciI CCGC 1 cut(s) 937
AclWI GGATC 1 cut(s) 257
AcsI RAATTY 4 cut(s) 228, 418, 643, 743
AcuI CTGAAG 2 cut(s) 537, 561
AfaI GTAC 8 cut(s) 13, 17, 22, 187, 266, 517, 812, 866
AfiI CCNNNNNNNGG 1 cut(s) 127
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 5 cut(s) 438, 455, 468, 748, 1228
AjnI CCWGG 2 cut(s) 388, 878
AluBI AGCT 9 cut(s) 47, 62, 111, 299, 767, 842, 981, 1187, 1245
AluI AGCT 9 cut(s) 47, 62, 111, 299, 767, 842, 981, 1187, 1245
Alw21I GWGCWC 3 cut(s) 347, 796, 1039
Alw26I GTCTC 3 cut(s) 223, 582, 708
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 1032
AoxI GGCC 2 cut(s) 27, 938
ApaLI GTGCAC 1 cut(s) 792
ApeKI GCWGC 5 cut(s) 111, 296, 446, 722, 896
ApoI RAATTY 4 cut(s) 228, 418, 643, 743
AseI ATTAAT 1 cut(s) 168
Asp718I GGTACC 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 876, 939
AsuHPI GGTGA 6 cut(s) 283, 420, 612, 639, 939, 998
AsuNHI GCTAGC 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 1032
AvaII GGWCC 1 cut(s) 876
BaeGI GKGCMC 1 cut(s) 796
BaeI ACNNNNGTAYC 1 cut(s) 32
BanI GGYRCC 1 cut(s) 185
Bbv12I GWGCWC 3 cut(s) 347, 796, 1039
BbvCI CCTCAGC 2 cut(s) 300, 684
BbvI GCAGC 5 cut(s) 98, 308, 433, 709, 908
BccI CCATC 1 cut(s) 908
BciT130I CCWGG 2 cut(s) 390, 880
BcoDI GTCTC 3 cut(s) 223, 582, 708
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 2 cut(s) 112, 570
BisI GCNGC 6 cut(s) 112, 297, 447, 723, 897, 938
BlsI GCNGC 6 cut(s) 113, 298, 448, 724, 898, 939
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 2 cut(s) 390, 880
Bme18I GGWCC 1 cut(s) 876
BmeT110I CYCGRG 1 cut(s) 1032
BmgT120I GGNCC 2 cut(s) 876, 939
BmiI GGNNCC 2 cut(s) 56, 187
BmrFI CCNGG 2 cut(s) 390, 880
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 3 cut(s) 734, 979, 1085
BmtI GCTAGC 1 cut(s) 51
BmuI ACTGGG 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 800, 832
BpmI CTGGAG 2 cut(s) 547, 866
Bpu10I CCTNAGC 2 cut(s) 300, 684
BpuEI CTTGAG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 70
BsaAI YACGTR 2 cut(s) 11, 15
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 4 cut(s) 258, 369, 650, 879
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 4 cut(s) 148, 423, 431, 538
Bse3DI GCAATG 1 cut(s) 1135
BseBI CCWGG 2 cut(s) 390, 880
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 258, 369, 650, 879
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 1135
BseMII CTCAG 4 cut(s) 225, 291, 410, 675
BseNI ACTGG 4 cut(s) 148, 423, 431, 538
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 5 cut(s) 98, 308, 433, 709, 908
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 2 cut(s) 24, 1121
Bsh1236I CGCG 1 cut(s) 937
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 29, 940
BshNI GGYRCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 3 cut(s) 347, 796, 1039
BsiHKCI CYCGRG 1 cut(s) 1032
BsiWI CGTACG 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 3 cut(s) 223, 582, 708
BsmI GAATGC 2 cut(s) 463, 1073
BsnI GGCC 2 cut(s) 29, 940
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 1 cut(s) 1032
Bsp1286I GDGCHC 3 cut(s) 347, 796, 1039
Bsp143I GATC 3 cut(s) 67, 71, 249
BspACI CCGC 1 cut(s) 937
BspANI GGCC 2 cut(s) 29, 940
BspCNI CTCAG 4 cut(s) 226, 292, 409, 676
BspDI ATCGAT 1 cut(s) 70
BspFNI CGCG 1 cut(s) 937
BspHI TCATGA 2 cut(s) 1006, 1231
BspLI GGNNCC 2 cut(s) 56, 187
BspMAI CTGCAG 2 cut(s) 116, 574
BspOI GCTAGC 1 cut(s) 51
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 879
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 1 cut(s) 1135
BsrI ACTGG 4 cut(s) 148, 423, 431, 538
BssECI CCNNGG 4 cut(s) 258, 369, 650, 879
BssMI GATC 3 cut(s) 67, 71, 249
BssT1I CCWWGG 2 cut(s) 258, 369
Bst2UI CCWGG 2 cut(s) 390, 880
Bst4CI ACNGT 6 cut(s) 20, 478, 757, 962, 1109, 1121
Bst6I CTCTTC 4 cut(s) 150, 282, 879, 1199
BstAPI GCANNNNNTGC 1 cut(s) 328
BstBAI YACGTR 2 cut(s) 11, 15
BstC8I GCNNGC 4 cut(s) 49, 53, 333, 681
BstDEI CTNAG 9 cut(s) 234, 300, 396, 591, 627, 672, 684, 965, 1191
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 1 cut(s) 937
BstKTI GATC 3 cut(s) 70, 74, 252
BstMAI GTCTC 3 cut(s) 223, 582, 708
BstMBI GATC 3 cut(s) 67, 71, 249
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 5 cut(s) 328, 545, 848, 1056, 1134
BstNI CCWGG 2 cut(s) 390, 880
BstNSI RCATGY 2 cut(s) 522, 683
BstSCI CCNGG 2 cut(s) 388, 878
BstSFI CTRYAG 2 cut(s) 112, 570
BstSLI GKGCMC 1 cut(s) 796
BstSNI TACGTA 2 cut(s) 11, 15
BstUI CGCG 1 cut(s) 937
BstV1I GCAGC 5 cut(s) 98, 308, 433, 709, 908
BstXI CCANNNNNNTGG 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 70
BsuRI GGCC 2 cut(s) 29, 940
BsuTUI ATCGAT 1 cut(s) 70
BtsCI GGATG 1 cut(s) 484
BtsI GCAGTG 3 cut(s) 567, 790, 1071
BtsIMutI CAGTG 3 cut(s) 567, 790, 1071
Cac8I GCNNGC 4 cut(s) 49, 53, 333, 681
CciI TCATGA 2 cut(s) 1006, 1231
Cfr13I GGNCC 2 cut(s) 876, 939
ClaI ATCGAT 1 cut(s) 70
Csp6I GTAC 8 cut(s) 12, 16, 21, 186, 265, 516, 811, 865
CviAII CATG 7 cut(s) 90, 127, 215, 519, 680, 1007, 1232
CviQI GTAC 8 cut(s) 12, 16, 21, 186, 265, 516, 811, 865
DdeI CTNAG 9 cut(s) 234, 300, 396, 591, 627, 672, 684, 965, 1191
DpnI GATC 3 cut(s) 69, 73, 251
DpnII GATC 3 cut(s) 67, 71, 249
DraI TTTAAA 2 cut(s) 417, 778
DrdI GACNNNNNNGTC 1 cut(s) 1119
DseDI GACNNNNNNGTC 1 cut(s) 1119
Eam1104I CTCTTC 4 cut(s) 150, 282, 879, 1199
EarI CTCTTC 4 cut(s) 150, 282, 879, 1199
Eco105I TACGTA 2 cut(s) 11, 15
Eco130I CCWWGG 2 cut(s) 258, 369
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 876
Eco57I CTGAAG 2 cut(s) 537, 561
Eco88I CYCGRG 1 cut(s) 1032
EcoRII CCWGG 2 cut(s) 388, 878
EcoT14I CCWWGG 2 cut(s) 258, 369
ErhI CCWWGG 2 cut(s) 258, 369
FaeI CATG 7 cut(s) 93, 130, 218, 522, 683, 1010, 1235
FatI CATG 7 cut(s) 89, 126, 214, 518, 679, 1006, 1231
FblI GTMKAC 2 cut(s) 1111, 1215
Fnu4HI GCNGC 6 cut(s) 112, 297, 447, 723, 897, 938
FokI GGATG 1 cut(s) 471
Fsp4HI GCNGC 6 cut(s) 112, 297, 447, 723, 897, 938
FspBI CTAG 1 cut(s) 48
GluI GCNGC 6 cut(s) 112, 297, 447, 723, 897, 938
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 2 cut(s) 547, 866
HaeIII GGCC 2 cut(s) 29, 940
Hin1II CATG 7 cut(s) 93, 130, 218, 522, 683, 1010, 1235
HindIII AAGCTT 1 cut(s) 1243
HinfI GANTC 9 cut(s) 86, 240, 254, 278, 349, 575, 617, 869, 1228
HphI GGTGA 6 cut(s) 283, 420, 612, 639, 939, 998
Hpy166II GTNNAC 6 cut(s) 428, 788, 794, 1112, 1197, 1216
Hpy188I TCNGA 5 cut(s) 82, 235, 474, 556, 637
Hpy188III TCNNGA 6 cut(s) 314, 353, 660, 845, 1007, 1232
Hpy8I GTNNAC 6 cut(s) 428, 788, 794, 1112, 1197, 1216
HpyAV CCTTC 5 cut(s) 98, 145, 268, 302, 780
HpyCH4III ACNGT 6 cut(s) 20, 478, 757, 962, 1109, 1121
HpyCH4IV ACGT 5 cut(s) 10, 14, 790, 1114, 1212
HpyF10VI GCNNNNNNNGC 5 cut(s) 328, 545, 848, 1056, 1134
HpyF3I CTNAG 9 cut(s) 234, 300, 396, 591, 627, 672, 684, 965, 1191
HpySE526I ACGT 5 cut(s) 10, 14, 790, 1114, 1212
Hsp92II CATG 7 cut(s) 93, 130, 218, 522, 683, 1010, 1235
KpnI GGTACC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 67, 71, 249
LguI GCTCTTC 1 cut(s) 879
LmnI GCTCC 3 cut(s) 60, 772, 1192
Lsp1109I GCAGC 5 cut(s) 98, 308, 433, 709, 908
LweI GCATC 3 cut(s) 734, 979, 1085
MaeI CTAG 1 cut(s) 48
MaeII ACGT 5 cut(s) 10, 14, 790, 1114, 1212
MaeIII GTNAC 6 cut(s) 499, 618, 751, 945, 1043, 1115
MalI GATC 3 cut(s) 69, 73, 251
MboI GATC 3 cut(s) 67, 71, 249
MhlI GDGCHC 3 cut(s) 347, 796, 1039
MluCI AATT 7 cut(s) 138, 165, 228, 418, 643, 697, 743
MlyI GAGTC 4 cut(s) 263, 272, 584, 626
MmeI TCCRAC 1 cut(s) 739
MseI TTAA 5 cut(s) 168, 339, 416, 777, 824
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 62, 299
MspR9I CCNGG 2 cut(s) 390, 880
Mva1269I GAATGC 2 cut(s) 463, 1073
MvaI CCWGG 2 cut(s) 390, 880
MvnI CGCG 1 cut(s) 937
MwoI GCNNNNNNNGC 5 cut(s) 328, 545, 848, 1056, 1134
NdeII GATC 3 cut(s) 67, 71, 249
NheI GCTAGC 1 cut(s) 47
NlaIII CATG 7 cut(s) 93, 130, 218, 522, 683, 1010, 1235
NlaIV GGNNCC 2 cut(s) 56, 187
NmuCI GTSAC 3 cut(s) 618, 945, 1043
NspI RCATGY 2 cut(s) 522, 683
PaeI GCATGC 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 1032
PagI TCATGA 2 cut(s) 1006, 1231
PciI ACATGT 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 879
PcsI WCGNNNNNNNCGW 2 cut(s) 658, 1039
PctI GAATGC 2 cut(s) 463, 1073
PfeI GAWTC 5 cut(s) 86, 240, 349, 869, 1228
Pfl23II CGTACG 1 cut(s) 11
PkrI GCNGC 6 cut(s) 113, 298, 448, 724, 898, 939
Ple19I CGATCG 1 cut(s) 74
PleI GAGTC 4 cut(s) 262, 272, 583, 625
PpsI GAGTC 4 cut(s) 262, 272, 583, 625
Ppu21I YACGTR 2 cut(s) 11, 15
PscI ACATGT 1 cut(s) 518
PshBI ATTAAT 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 388, 878
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 388, 878
PspLI CGTACG 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 56, 187
PspPI GGNCC 2 cut(s) 876, 939
PspXI VCTCGAGB 1 cut(s) 1032
PstI CTGCAG 2 cut(s) 116, 574
PvuI CGATCG 1 cut(s) 74
PvuII CAGCTG 2 cut(s) 62, 299
RsaI GTAC 8 cut(s) 13, 17, 22, 187, 266, 517, 812, 866
RsaNI GTAC 8 cut(s) 12, 16, 21, 186, 265, 516, 811, 865
RseI CAYNNNNRTG 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 879
SaqAI TTAA 5 cut(s) 168, 339, 416, 777, 824
SatI GCNGC 6 cut(s) 112, 297, 447, 723, 897, 938
Sau3AI GATC 3 cut(s) 67, 71, 249
Sau96I GGNCC 2 cut(s) 876, 939
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 4 cut(s) 263, 272, 584, 626
ScrFI CCNGG 2 cut(s) 390, 880
SduI GDGCHC 3 cut(s) 347, 796, 1039
SfaNI GCATC 3 cut(s) 734, 979, 1085
SfcI CTRYAG 2 cut(s) 112, 570
Sfr274I CTCGAG 1 cut(s) 1032
SinI GGWCC 1 cut(s) 876
SlaI CTCGAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 314, 1032
SmoI CTYRAG 2 cut(s) 314, 1032
SnaBI TACGTA 2 cut(s) 11, 15
SphI GCATGC 1 cut(s) 683
Sse9I AATT 7 cut(s) 138, 165, 228, 418, 643, 697, 743
SsiI CCGC 1 cut(s) 937
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 388, 878
StyI CCWWGG 2 cut(s) 258, 369
TaaI ACNGT 6 cut(s) 20, 478, 757, 962, 1109, 1121
TaiI ACGT 5 cut(s) 13, 17, 793, 1117, 1215
TaqI TCGA 5 cut(s) 70, 252, 1033, 1079, 1239
TaqII GACCGA 1 cut(s) 642
TasI AATT 7 cut(s) 138, 165, 228, 418, 643, 697, 743
TatI WGTACW 3 cut(s) 20, 264, 810
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 5 cut(s) 86, 240, 349, 869, 1228
Tru1I TTAA 5 cut(s) 168, 339, 416, 777, 824
Tru9I TTAA 5 cut(s) 168, 339, 416, 777, 824
TscAI CASTG 3 cut(s) 574, 790, 1078
TseFI GTSAC 3 cut(s) 618, 945, 1043
TseI GCWGC 5 cut(s) 111, 296, 446, 722, 896
Tsp45I GTSAC 3 cut(s) 618, 945, 1043
TspDTI ATGAA 4 cut(s) 78, 117, 300, 1248
TspRI CASTG 3 cut(s) 574, 790, 1078
VneI GTGCAC 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 876
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 4 cut(s) 228, 418, 643, 743
XceI RCATGY 2 cut(s) 522, 683
XhoI CTCGAG 1 cut(s) 1032
XmiI GTMKAC 2 cut(s) 1111, 1215
XspI CTAG 1 cut(s) 48
ZrmI AGTACT 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.