Rroxscaffold_6G00428190

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005029
Physical Location & Seq
Forward (+)
48679069 .. 48683834
4766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428190.1

Sequence Viewer

Length: 1371 bp
ATGTGTTTCCTGGAAGAGAAGAAAATTAATGGTGTTGTAGTGGGTACCAATGGTTTTACTACTGCTAAAATCCATGTCTCTCCCAAAATTTCCGAGAGAATCAGCAGGATCGAGTCCAAGGAGTACAAGGTGAAGGACTCTAATGAAGAGAAAGCAGCTGAGGGGGAAGGTCTTGAGAAGCAAGAGATGCTGGCTGTTAAGAGCACGAATCTTGATGATGACTTGACCAAGGAGAATAATGAGAAACCTGGAACTCAGATGTCAAGTGAGAGTTTAAATTCCAGTTCACCAGTTTCAAAATCTGCTGCTGTTGAAAATGGGCATTCAAATCAGACTGTTCCTCATCCCAATGCTCTGGTAACTGAAAGGGCTGGTACATGTGAAGAGACTGCTGGGGCTGAAGTTGCCACTTCAGAAAACTCCAGCACTGCAGAGTCTCCTAATGCAACTAAGACTTCACAGACTGCCAATGCAGAGTCACCCACTAAGAGTTCAGAGCCAAATTCACCTCGGTCATCACGACCTGATACTAAGAAGCATGCTGAGGAAGAAGATAATTGGTCTCTTACTTCCTCAACTGCTGCATCTGTGAGAACAAATAAATTCAAGGTTACAGTTGGACAAGCTCCTTCGTTTAAATGCAGTGAACGCGCACAAAAAAGAAATGAGTACTACTCAAAGTTAAAGGAAAAACAACAAGCTCTGGAGGCAGAGAGATTGCAGTACGAATCAAGGACCAGGGAAGAGCAAGAAGCAGCCATCAAGCAGCTGAGGAAGTCCTTGGTGATCAAAGCAAAGCCAGTACCTAGTTTCTATTACGAGCCACCTCCTCCTAAGGCTGAACTCAAGAAGCTTCCACTTACGCGGCCCAAGTCACCAAAACTGAACAGTCTAAGCAGGAGAAAGAGCTTTGGTGATGCTCTCAACACATCTCATGACGAAAAGGGAAGAGTTTGTCCTCGAGCACATCGTCACAGCGTTGGTTGCCAGAAAGAATGCACTGCATCGACTACTCCAAAGAATAAGGATCTGTCGATCTGCCTCTCTGATGTGGGGGATGTTGCTGTTCTGGGTGCGGCTCTTCCAACTAATACCCTTGGTATCCCCTGCAACTTTGATCCGCACAGAAGGGCTGGTATATGCGGTGATTGGTTACTTGGTTCGAGTTTAGAATCAGCAGCCATAAGCGGAATGACTCTTGCAAATCATATCGAGGACTATTTCCAAAGCGGTGGAGACCGCCCCGAAGAGTTTGCTTTTGGTTTACGCATTGAGTTTGAACGACTTAAAGGACATGATATAGGCCAATTTCCAGGGTTAGAGCCCGTGAGAGAAAGACATACAGCGCCAGAATTGCAGCTTACCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

456

Amino Acids

49.94

Weight (kDa)

7.16

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 211 - 283 9.6e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 2 cut(s) 651, 865
AciI CCGC 7 cut(s) 865, 1076, 1121, 1143, 1188, 1230, 1240
AclWI GGATC 3 cut(s) 116, 1035, 1112
AcsI RAATTY 4 cut(s) 87, 277, 502, 602
AcuI CTGAAG 2 cut(s) 396, 420
AfaI GTAC 6 cut(s) 46, 125, 376, 671, 725, 804
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 5 cut(s) 297, 314, 327, 607, 1280
AjnI CCWGG 4 cut(s) 9, 247, 737, 1312
AluBI AGCT 7 cut(s) 158, 626, 701, 769, 853, 909, 1360
AluI AGCT 7 cut(s) 158, 626, 701, 769, 853, 909, 1360
Alw21I GWGCWC 2 cut(s) 206, 967
Alw26I GTCTC 5 cut(s) 82, 380, 441, 567, 1230
AlwI GGATC 3 cut(s) 116, 1035, 1112
Ama87I CYCGRG 1 cut(s) 960
AoxI GGCC 2 cut(s) 866, 1303
ApeKI GCWGC 7 cut(s) 155, 305, 581, 755, 766, 1178, 1357
ApoI RAATTY 4 cut(s) 87, 277, 502, 602
AseI ATTAAT 1 cut(s) 27
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 2 cut(s) 653, 1348
AspS9I GGNCC 2 cut(s) 735, 867
AsuHPI GGTGA 8 cut(s) 142, 279, 471, 498, 796, 867, 926, 1157
AvaI CYCGRG 1 cut(s) 960
AvaII GGWCC 1 cut(s) 735
AxyI CCTNAGG 1 cut(s) 834
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 1326
Bbv12I GWGCWC 2 cut(s) 206, 967
BbvCI CCTCAGC 3 cut(s) 159, 543, 770
BbvI GCAGC 6 cut(s) 167, 292, 568, 767, 778, 1190
BccI CCATC 1 cut(s) 767
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 4 cut(s) 11, 249, 739, 1314
BciVI GTATCC 1 cut(s) 1112
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 5 cut(s) 82, 380, 441, 567, 1230
BfaI CTAG 1 cut(s) 807
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 1349
BfuI GTATCC 1 cut(s) 1112
BisI GCNGC 9 cut(s) 156, 306, 582, 756, 767, 866, 1077, 1179, 1358
BlsI GCNGC 9 cut(s) 157, 307, 583, 757, 768, 867, 1078, 1180, 1359
BmcAI AGTACT 1 cut(s) 671
Bme1390I CCNGG 4 cut(s) 11, 249, 739, 1314
Bme18I GGWCC 1 cut(s) 735
BmeT110I CYCGRG 1 cut(s) 960
BmgT120I GGNCC 2 cut(s) 735, 867
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 4 cut(s) 11, 249, 739, 1314
BmsI GCATC 4 cut(s) 177, 593, 907, 1013
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpmI CTGGAG 2 cut(s) 406, 725
Bpu10I CCTNAGC 3 cut(s) 159, 543, 770
BpuEI CTTGAG 2 cut(s) 194, 830
BsaI GGTCTC 2 cut(s) 567, 1230
BsaJI CCNNGG 7 cut(s) 117, 228, 509, 738, 780, 1096, 1313
Bse1I ACTGG 3 cut(s) 282, 290, 800
Bse21I CCTNAGG 1 cut(s) 834
BseBI CCWGG 4 cut(s) 11, 249, 739, 1314
BseDI CCNNGG 7 cut(s) 117, 228, 509, 738, 780, 1096, 1313
BseGI GGATG 2 cut(s) 343, 1063
BseMII CTCAG 4 cut(s) 150, 269, 534, 761
BseNI ACTGG 3 cut(s) 282, 290, 800
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 6 cut(s) 167, 292, 568, 767, 778, 1190
BseYI CCCAGC 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 651, 865
BshFI GGCC 2 cut(s) 868, 1305
BshNI GGYRCC 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 206, 967
BsiHKCI CYCGRG 1 cut(s) 960
BsmAI GTCTC 5 cut(s) 82, 380, 441, 567, 1230
BsmI GAATGC 2 cut(s) 322, 1001
BsnI GGCC 2 cut(s) 868, 1305
Bso31I GGTCTC 2 cut(s) 567, 1230
BsoBI CYCGRG 1 cut(s) 960
Bsp1286I GDGCHC 3 cut(s) 206, 967, 1326
Bsp143I GATC 5 cut(s) 108, 786, 1027, 1035, 1117
BspACI CCGC 7 cut(s) 865, 1076, 1121, 1143, 1188, 1230, 1240
BspANI GGCC 2 cut(s) 868, 1305
BspCNI CTCAG 4 cut(s) 151, 268, 535, 762
BspFNI CGCG 2 cut(s) 651, 865
BspHI TCATGA 1 cut(s) 934
BspLI GGNNCC 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 3 cut(s) 116, 1035, 1112
BspQI GCTCTTC 2 cut(s) 738, 1086
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 2 cut(s) 567, 1230
BsrI ACTGG 3 cut(s) 282, 290, 800
BssECI CCNNGG 7 cut(s) 117, 228, 509, 738, 780, 1096, 1313
BssMI GATC 5 cut(s) 108, 786, 1027, 1035, 1117
BssT1I CCWWGG 4 cut(s) 117, 228, 780, 1096
Bst2UI CCWGG 4 cut(s) 11, 249, 739, 1314
Bst4CI ACNGT 3 cut(s) 337, 616, 890
Bst6I CTCTTC 7 cut(s) 9, 141, 378, 738, 943, 1086, 1242
BstAPI GCANNNNNTGC 1 cut(s) 187
BstC8I GCNNGC 2 cut(s) 192, 540
BstDEI CTNAG 9 cut(s) 159, 255, 450, 486, 531, 543, 770, 834, 893
BstF5I GGATG 2 cut(s) 343, 1063
BstFNI CGCG 2 cut(s) 651, 865
BstH2I RGCGCY 1 cut(s) 1349
BstHHI GCGC 2 cut(s) 653, 1348
BstKTI GATC 5 cut(s) 111, 789, 1030, 1038, 1120
BstMAI GTCTC 5 cut(s) 82, 380, 441, 567, 1230
BstMBI GATC 5 cut(s) 108, 786, 1027, 1035, 1117
BstMWI GCNNNNNNNGC 6 cut(s) 187, 404, 648, 707, 984, 1354
BstNI CCWGG 4 cut(s) 11, 249, 739, 1314
BstNSI RCATGY 2 cut(s) 381, 542
BstSCI CCNGG 4 cut(s) 9, 247, 737, 1312
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 2 cut(s) 651, 865
BstV1I GCAGC 6 cut(s) 167, 292, 568, 767, 778, 1190
BstX2I RGATCY 1 cut(s) 1027
BstXI CCANNNNNNTGG 2 cut(s) 355, 1232
BstYI RGATCY 1 cut(s) 1027
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 1112
BsuRI GGCC 2 cut(s) 868, 1305
BtsCI GGATG 2 cut(s) 343, 1063
BtsI GCAGTG 3 cut(s) 426, 649, 999
BtsIMutI CAGTG 3 cut(s) 426, 649, 999
Cac8I GCNNGC 2 cut(s) 192, 540
CciI TCATGA 1 cut(s) 934
CfoI GCGC 2 cut(s) 653, 1348
Cfr13I GGNCC 2 cut(s) 735, 867
Csp6I GTAC 6 cut(s) 45, 124, 375, 670, 724, 803
CviAII CATG 5 cut(s) 74, 378, 539, 935, 1295
CviQI GTAC 6 cut(s) 45, 124, 375, 670, 724, 803
DdeI CTNAG 9 cut(s) 159, 255, 450, 486, 531, 543, 770, 834, 893
DpnI GATC 5 cut(s) 110, 788, 1029, 1037, 1119
DpnII GATC 5 cut(s) 108, 786, 1027, 1035, 1117
DraI TTTAAA 2 cut(s) 276, 637
Eam1104I CTCTTC 7 cut(s) 9, 141, 378, 738, 943, 1086, 1242
EarI CTCTTC 7 cut(s) 9, 141, 378, 738, 943, 1086, 1242
Eco130I CCWWGG 4 cut(s) 117, 228, 780, 1096
Eco24I GRGCYC 1 cut(s) 1326
Eco31I GGTCTC 2 cut(s) 567, 1230
Eco47I GGWCC 1 cut(s) 735
Eco57I CTGAAG 2 cut(s) 396, 420
Eco81I CCTNAGG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 960
EcoRII CCWGG 4 cut(s) 9, 247, 737, 1312
EcoT14I CCWWGG 4 cut(s) 117, 228, 780, 1096
EcoT38I GRGCYC 1 cut(s) 1326
ErhI CCWWGG 4 cut(s) 117, 228, 780, 1096
FaeI CATG 5 cut(s) 77, 381, 542, 938, 1298
FatI CATG 5 cut(s) 73, 377, 538, 934, 1294
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 9 cut(s) 156, 306, 582, 756, 767, 866, 1077, 1179, 1358
FokI GGATG 2 cut(s) 330, 1070
FriOI GRGCYC 1 cut(s) 1326
Fsp4HI GCNGC 9 cut(s) 156, 306, 582, 756, 767, 866, 1077, 1179, 1358
FspBI CTAG 1 cut(s) 807
GlaI GCGC 2 cut(s) 652, 1347
GluI GCNGC 9 cut(s) 156, 306, 582, 756, 767, 866, 1077, 1179, 1358
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 2 cut(s) 406, 725
HaeII RGCGCY 1 cut(s) 1349
HaeIII GGCC 2 cut(s) 868, 1305
HhaI GCGC 2 cut(s) 653, 1348
Hin1II CATG 5 cut(s) 77, 381, 542, 938, 1298
Hin6I GCGC 2 cut(s) 651, 1346
HinP1I GCGC 2 cut(s) 651, 1346
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 9 cut(s) 99, 113, 137, 208, 434, 476, 728, 1172, 1195
HphI GGTGA 8 cut(s) 142, 279, 471, 498, 796, 867, 926, 1157
Hpy166II GTNNAC 3 cut(s) 287, 647, 1265
Hpy188I TCNGA 6 cut(s) 94, 258, 333, 415, 496, 1048
Hpy188III TCNNGA 6 cut(s) 173, 212, 519, 704, 847, 935
Hpy8I GTNNAC 3 cut(s) 287, 647, 1265
HpyAV CCTTC 4 cut(s) 127, 161, 639, 1122
HpyCH4III ACNGT 3 cut(s) 337, 616, 890
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 404, 648, 707, 984, 1354
HpyF3I CTNAG 9 cut(s) 159, 255, 450, 486, 531, 543, 770, 834, 893
Hsp92II CATG 5 cut(s) 77, 381, 542, 938, 1298
HspAI GCGC 2 cut(s) 651, 1346
KpnI GGTACC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 5 cut(s) 108, 786, 1027, 1035, 1117
LguI GCTCTTC 2 cut(s) 738, 1086
LmnI GCTCC 1 cut(s) 631
Lsp1109I GCAGC 6 cut(s) 167, 292, 568, 767, 778, 1190
LweI GCATC 4 cut(s) 177, 593, 907, 1013
MaeI CTAG 1 cut(s) 807
MaeIII GTNAC 6 cut(s) 358, 477, 610, 873, 971, 1152
MalI GATC 5 cut(s) 110, 788, 1029, 1037, 1119
MboI GATC 5 cut(s) 108, 786, 1027, 1035, 1117
MflI RGATCY 1 cut(s) 1027
MhlI GDGCHC 3 cut(s) 206, 967, 1326
MluCI AATT 8 cut(s) 24, 87, 277, 502, 556, 602, 1307, 1352
MlyI GAGTC 5 cut(s) 122, 131, 443, 485, 1189
MmeI TCCRAC 2 cut(s) 598, 1109
MseI TTAA 7 cut(s) 27, 198, 275, 636, 683, 1287, 1369
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 2 cut(s) 158, 769
MspR9I CCNGG 4 cut(s) 11, 249, 739, 1314
Mva1269I GAATGC 2 cut(s) 322, 1001
MvaI CCWGG 4 cut(s) 11, 249, 739, 1314
MvnI CGCG 2 cut(s) 651, 865
MwoI GCNNNNNNNGC 6 cut(s) 187, 404, 648, 707, 984, 1354
NdeII GATC 5 cut(s) 108, 786, 1027, 1035, 1117
NlaIII CATG 5 cut(s) 77, 381, 542, 938, 1298
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 3 cut(s) 477, 873, 971
NspI RCATGY 2 cut(s) 381, 542
PaeI GCATGC 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 960
PagI TCATGA 1 cut(s) 934
PciI ACATGT 1 cut(s) 377
PciSI GCTCTTC 2 cut(s) 738, 1086
PcsI WCGNNNNNNNCGW 2 cut(s) 517, 967
PctI GAATGC 2 cut(s) 322, 1001
PfeI GAWTC 4 cut(s) 99, 208, 728, 1172
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 9 cut(s) 157, 307, 583, 757, 768, 867, 1078, 1180, 1359
PleI GAGTC 5 cut(s) 121, 131, 442, 484, 1189
PpsI GAGTC 5 cut(s) 121, 131, 442, 484, 1189
PscI ACATGT 1 cut(s) 377
PshBI ATTAAT 1 cut(s) 27
Psp6I CCWGG 4 cut(s) 9, 247, 737, 1312
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 4 cut(s) 9, 247, 737, 1312
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 2 cut(s) 735, 867
PspXI VCTCGAGB 1 cut(s) 960
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 1027
PvuII CAGCTG 2 cut(s) 158, 769
RsaI GTAC 6 cut(s) 46, 125, 376, 671, 725, 804
RsaNI GTAC 6 cut(s) 45, 124, 375, 670, 724, 803
RseI CAYNNNNRTG 1 cut(s) 348
SapI GCTCTTC 2 cut(s) 738, 1086
SaqAI TTAA 7 cut(s) 27, 198, 275, 636, 683, 1287, 1369
SatI GCNGC 9 cut(s) 156, 306, 582, 756, 767, 866, 1077, 1179, 1358
Sau3AI GATC 5 cut(s) 108, 786, 1027, 1035, 1117
Sau96I GGNCC 2 cut(s) 735, 867
ScaI AGTACT 1 cut(s) 671
SchI GAGTC 5 cut(s) 122, 131, 443, 485, 1189
ScrFI CCNGG 4 cut(s) 11, 249, 739, 1314
SduI GDGCHC 3 cut(s) 206, 967, 1326
SfaNI GCATC 4 cut(s) 177, 593, 907, 1013
SfcI CTRYAG 1 cut(s) 429
Sfr274I CTCGAG 1 cut(s) 960
SinI GGWCC 1 cut(s) 735
SlaI CTCGAG 1 cut(s) 960
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 173, 845, 960
SmoI CTYRAG 3 cut(s) 173, 845, 960
SphI GCATGC 1 cut(s) 542
Sse9I AATT 8 cut(s) 24, 87, 277, 502, 556, 602, 1307, 1352
SsiI CCGC 7 cut(s) 865, 1076, 1121, 1143, 1188, 1230, 1240
SspMI CTAG 1 cut(s) 807
StyD4I CCNGG 4 cut(s) 9, 247, 737, 1312
StyI CCWWGG 4 cut(s) 117, 228, 780, 1096
TaaI ACNGT 3 cut(s) 337, 616, 890
TaqI TCGA 6 cut(s) 111, 961, 1007, 1034, 1163, 1212
TaqII GACCGA 1 cut(s) 501
TasI AATT 8 cut(s) 24, 87, 277, 502, 556, 602, 1307, 1352
TatI WGTACW 2 cut(s) 123, 669
TauI GCSGC 2 cut(s) 868, 1079
TfiI GAWTC 4 cut(s) 99, 208, 728, 1172
Tru1I TTAA 7 cut(s) 27, 198, 275, 636, 683, 1287, 1369
Tru9I TTAA 7 cut(s) 27, 198, 275, 636, 683, 1287, 1369
TscAI CASTG 3 cut(s) 433, 649, 1006
TseFI GTSAC 3 cut(s) 477, 873, 971
TseI GCWGC 7 cut(s) 155, 305, 581, 755, 766, 1178, 1357
Tsp45I GTSAC 3 cut(s) 477, 873, 971
TspDTI ATGAA 1 cut(s) 159
TspRI CASTG 3 cut(s) 433, 649, 1006
VpaK11BI GGWCC 1 cut(s) 735
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 4 cut(s) 87, 277, 502, 602
XceI RCATGY 2 cut(s) 381, 542
XhoI CTCGAG 1 cut(s) 960
XspI CTAG 1 cut(s) 807
ZrmI AGTACT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.