Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0004945

Physical Location & Seq

Genomic Coordinates Forward (+)

2171822 .. 2182207

Gene Structure 10386 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g03990.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2115 bp

ATGACATCAGTTATAGGTTTTGGGGGACCTCACTTTTTTGGGTTGAGTTCCGAGAGTAAGCCTCGGTTTAGAGTGCAAGGTAGAAGTTTAAGTAATCAGGATGCATCAGTTTTGTCTTCAGATTCAGTTGTAGTGAATGGAGCTTCTGTAGTTGGGGAGAGGGAGGGAAGTAGGCCTTTAGTTGATCAAGGGAATGGCAGTTTGAGGCATAAGGTTAAGGAGAAACAGAAGATAGAAGATGGTGTTCTTGAAAGTTTGGAACCTTTGTGGGATGACGGGTATGGGACTGTGACTGTAAAGGATTATTTCGATGCAAATGAGCAGATGATTAAGCCTGATGGTGGTCCTCCCCGATGGTTCAGCCCCCTTGCGTTTAACCCTCCATTGAAGGGTTCTCCAGTTCTTTTGTTTTTGCCTGGGCTGGATGGTACTGGCTGGGGACTCATTTTGCATCATAAAGCTCTTCAGAAGGTCTTTGAAGTTCGATGCTTGCATATTCCAGTTAACGATCGAACACCGTTTGAAGGGCTGGTGAAATTGGTTGAAGAAACTATTAGGCTCGAGCATGCTTCATCTCCCAACAAGCCAATATATTTGGTAGGAGATTCTTTTGGAGGATGCCTCTCACTTGCTGTGGCTGCACGTAATCCTACCATTGACCTCGTACTGGTACTAGTCAACCCAGCAACATCCATTGAGAGGTCTCAGTTGCAACCTCTCTTCCCAATCTTGGAGGCTATGCCTGAAGAATTACATATTACAGTTCCCTATCTTCTCAGCTTTGTTATGGGTGATCCTACAAAAATGGCAATGGTGAACATCAAAAGTAGGCTTCCTCCAGGTTTAAAACTTGCGCAAATGTCTCAGAACCTCACTGCTTTGCTACCATGTCTCTCTTCCTTGGCCGATATTATACCAAGGGAAACTCTTCTTTGGAAGCTGAAGCTGCTCAAATCAGCTGCTGGTTATGCAAATTCCCGTCTCCATGCTGTCAAAGCTGAAGTACTTGTGTTGGCTAGTGACAAGGATAACATGGTTCCTAGTCAAGATGAAGCGGGCCGATTAAGGAGCGCATTACAGAACTGTACAGTTCGTCACTTCCCAGACAATGGGCATACCCTATTACTGGAAGATGGGGTTAATTTGTTGACAGTAATAAAAGGTACTCTCAAATATCGACGTTCGAGGAAGCGTGATAGTTTCTCAGATTTTCTTCCTCCTAGTATGACAGAATTCAACAATACATTTGATGAAACAGTCGGATTGTTACGCTTTGCTTCTGGTGCTGTTCTTTTCTCAACTTTGGAAGATGGAACGGTGGTGAGAGGTCTTGCAGGAGTTCCAAATGAAGGACCTGTCTTAATTGTTGGCTATCATAATCTAATGGGGCTGGAACTGTATTCTATGGTTGAAGAATTTTTGAGAGAAAAGAAAATAATGGTTCGTGGTTTAGCACATCCAGAACTGTTGTTGGGGAAGAGCTCAGATGATTTTGACTTGCTCAAAGTATTTGGTGCAGTACCAGTTACGGCAAGATATCTATTCAAATCACTTTCATCAAAATCACATGTTCTCCTGTATCCTGGTGGTGCACGTGAAGCTCTCCATTACAAGGGTGAAGAATACAAGTTATTCTGGCCTGATCAACCTGAATTTGTCAGAATGGCAGCACGTTTTGGAGCCACAATTGTACCATTTGCAGCTGTAGGAGCAGATGACTTACTCGAATTGGTTATCGACTACAATGACATAAAGAATATCCCTGTGGTGGGCGACTATTTCATCAATGCTAATAATGGTGCTGTAAAATTGAGGGAGGATACAAGTGGAGAAGTTGCCAATCAAAACCTCTTTATTCCAGGAGTTCTACCTAAGTTGCCAGGCCGTTATTACCATTTGTTTGGGAAACCAATAGAAACAAAAGGAAGGGAAGAGATCCTGAAAGACAAAGACAGTGCAAACAAATTGTATTTGGAGATACAATCTGAAATTGAACAAAGTTTAGCATACTTAATCAAGAAGCGGGAGGAGGATCCTTACAGGAATATTATTGACAGAACAGTATATCGGGCACTATATTCCCCTCTAAATGAAGTTCCAACATTTGAACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGAT Hydrolase_4

Protein Analysis

705

Amino Acids

77.99

Weight (kDa)

6.16

Isoelectric Point (pI)

46.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	135 - 378	2.5e-06	Alpha/beta hydrolase family
Hydrolase_4	PF12146	182 - 377	1.4e-09	Serine aminopeptidase, S33
DAGAT	PF03982	513 - 580	7.9e-06	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54570
fragaria_vesca	FvH4_6g03990 FvH4_6g04000
malus_domestica	MD04G1216900.v1.1 MD12G1233500.v1.1
prunus_persica	Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1
pyrus_communis	pycom04g19300 pycom12g21610
rosa_chinensis	RchiOBHm_Chr3g0452581
rosa_laevigata	RLG00000014771 RLG00000025582
rosa_multiflora	Rmu_co8485569.1_g000001 Rmu_sc0004414.1_g000004 Rmu_sc0028651.1_g000001 Rmu_sc0032164.1_g000001
rosa_roxburghii	Rroxscaffold_6G00429620 Rroxscaffold_7G00209280 Rroxscaffold_7G00209300
rosa_rugosa	Rorug02G0641400 Rorug02G0641500 Rorug02G0641500
rosa_samantha	Rh3AG044000 Rh3BG046100 Rh3CG044800 Rh3DG045100
rosa_wichuraiana	Rw3G003410 Rw6G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	855
AccB7I	CCANNNNNTGG	1 cut(s)	1109
AciI	CCGC	2 cut(s)	1055, 2023
AclWI	GGATC	4 cut(s)	788, 1930, 2027, 2040
AcoI	YGGCCR	1 cut(s)	903
AcsI	RAATTY	4 cut(s)	973, 1232, 1415, 1652
AcuI	CTGAAG	5 cut(s)	102, 449, 765, 962, 1020
AcvI	CACGTG	1 cut(s)	1595
AfaI	GTAC	8 cut(s)	430, 666, 672, 1005, 1087, 1165, 1521, 1692
AflIII	ACRYGT	1 cut(s)	1567
AhlI	ACTAGT	1 cut(s)	673
AjnI	CCWGG	5 cut(s)	415, 838, 1582, 1858, 1879
Alw21I	GWGCWC	2 cut(s)	1484, 1594
Alw26I	GTCTC	4 cut(s)	708, 867, 896, 986
Alw44I	GTGCAC	1 cut(s)	1590
AlwI	GGATC	4 cut(s)	788, 1930, 2027, 2040
AlwNI	CAGNNNCTG	1 cut(s)	962
Ama87I	CYCGRG	1 cut(s)	560
AoxI	GGCC	5 cut(s)	173, 903, 1057, 1637, 1882
ApaLI	GTGCAC	1 cut(s)	1590
ApeKI	GCWGC	5 cut(s)	638, 946, 959, 1667, 1700
ApoI	RAATTY	4 cut(s)	973, 1232, 1415, 1652
Asp700I	GAANNNNTTC	1 cut(s)	927
AspLEI	GCGC	2 cut(s)	856, 1073
AspS9I	GGNCC	4 cut(s)	26, 344, 1057, 1352
AsuHPI	GGTGA	5 cut(s)	544, 803, 826, 1333, 1628
AvaI	CYCGRG	1 cut(s)	560
AvaII	GGWCC	3 cut(s)	26, 344, 1352
BaeGI	GKGCMC	2 cut(s)	1594, 2074
BaeI	ACNNNNGTAYC	2 cut(s)	1674, 1707
BamHI	GGATCC	1 cut(s)	2032
BanII	GRGCYC	1 cut(s)	1484
BbrPI	CACGTG	1 cut(s)	1595
BbsI	GAAGAC	1 cut(s)	108
Bbv12I	GWGCWC	2 cut(s)	1484, 1594
BbvI	GCAGC	5 cut(s)	625, 933, 946, 1679, 1712
BccI	CCATC	6 cut(s)	233, 332, 348, 419, 1127, 1304
BceAI	ACGGC	2 cut(s)	1545, 1869
BciT130I	CCWGG	5 cut(s)	417, 840, 1584, 1860, 1881
BciVI	GTATCC	2 cut(s)	1590, 1813
BclI	TGATCA	2 cut(s)	184, 1642
BcoDI	GTCTC	4 cut(s)	708, 867, 896, 986
BcuI	ACTAGT	1 cut(s)	673
BfaI	CTAG	4 cut(s)	674, 1017, 1041, 1221
BfmI	CTRYAG	2 cut(s)	147, 1704
BfuI	GTATCC	2 cut(s)	1590, 1813
BisI	GCNGC	5 cut(s)	639, 947, 960, 1668, 1701
BlsI	GCNGC	5 cut(s)	640, 948, 961, 1669, 1702
BmcAI	AGTACT	1 cut(s)	1005
Bme1390I	CCNGG	5 cut(s)	417, 840, 1584, 1860, 1881
Bme18I	GGWCC	3 cut(s)	26, 344, 1352
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	4 cut(s)	26, 344, 1057, 1352
BmiI	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
BmrFI	CCNGG	5 cut(s)	417, 840, 1584, 1860, 1881
BmsI	GCATC	6 cut(s)	91, 113, 301, 460, 476, 608
BpiI	GAAGAC	1 cut(s)	108
BplI	GAGNNNNNCTC	4 cut(s)	46, 78, 606, 638
BpmI	CTGGAG	2 cut(s)	381, 822
BsaAI	YACGTR	2 cut(s)	644, 1595
BsaI	GGTCTC	1 cut(s)	708
BsaJI	CCNNGG	4 cut(s)	62, 416, 900, 917
BsaXI	ACNNNNNCTCC	4 cut(s)	132, 162, 1557, 1587
Bse1I	ACTGG	6 cut(s)	398, 436, 500, 672, 1131, 1523
Bse3DI	GCAATG	1 cut(s)	816
BseBI	CCWGG	5 cut(s)	417, 840, 1584, 1860, 1881
BseDI	CCNNGG	4 cut(s)	62, 416, 900, 917
BseGI	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BseMI	GCAATG	1 cut(s)	816
BseMII	CTCAG	5 cut(s)	719, 790, 878, 1218, 1497
BseNI	ACTGG	6 cut(s)	398, 436, 500, 672, 1131, 1523
BseRI	GAGGAG	1 cut(s)	2042
BseSI	GKGCMC	2 cut(s)	1594, 2074
BseXI	GCAGC	5 cut(s)	625, 933, 946, 1679, 1712
BseYI	CCCAGC	2 cut(s)	435, 682
BsgI	GTGCAG	2 cut(s)	624, 1536
Bsh1285I	CGRYCG	1 cut(s)	511
BshFI	GGCC	5 cut(s)	175, 905, 1059, 1639, 1884
BsiEI	CGRYCG	1 cut(s)	511
BsiHKAI	GWGCWC	2 cut(s)	1484, 1594
BsiHKCI	CYCGRG	1 cut(s)	560
BslFI	GGGAC	3 cut(s)	39, 298, 453
BsmAI	GTCTC	4 cut(s)	708, 867, 896, 986
BsmBI	CGTCTC	1 cut(s)	986
BsmFI	GGGAC	3 cut(s)	39, 298, 453
BsnI	GGCC	5 cut(s)	175, 905, 1059, 1639, 1884
Bso31I	GGTCTC	1 cut(s)	708
BsoBI	CYCGRG	1 cut(s)	560
Bsp1286I	GDGCHC	3 cut(s)	1484, 1594, 2074
Bsp1407I	TGTACA	1 cut(s)	1085
Bsp143I	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
BspACI	CCGC	2 cut(s)	1055, 2023
BspANI	GGCC	5 cut(s)	175, 905, 1059, 1639, 1884
BspCNI	CTCAG	5 cut(s)	718, 789, 877, 1217, 1496
BspLI	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
BspPI	GGATC	4 cut(s)	788, 1930, 2027, 2040
BspQI	GCTCTTC	2 cut(s)	468, 1472
BspTNI	GGTCTC	1 cut(s)	708
BsrDI	GCAATG	1 cut(s)	816
BsrGI	TGTACA	1 cut(s)	1085
BsrI	ACTGG	6 cut(s)	398, 436, 500, 672, 1131, 1523
BssECI	CCNNGG	4 cut(s)	62, 416, 900, 917
BssMI	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
BssT1I	CCWWGG	2 cut(s)	900, 917
Bst2UI	CCWGG	5 cut(s)	417, 840, 1584, 1860, 1881
Bst6I	CTCTTC	6 cut(s)	468, 725, 901, 933, 1472, 1926
BstAUI	TGTACA	1 cut(s)	1085
BstBAI	YACGTR	2 cut(s)	644, 1595
BstC8I	GCNNGC	3 cut(s)	491, 567, 1057
BstDEI	CTNAG	6 cut(s)	705, 776, 864, 1204, 1483, 1872
BstF5I	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BstHHI	GCGC	2 cut(s)	856, 1073
BstKTI	GATC	6 cut(s)	187, 511, 796, 1645, 1938, 2035
BstMAI	GTCTC	4 cut(s)	708, 867, 896, 986
BstMBI	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
BstMCI	CGRYCG	1 cut(s)	511
BstMWI	GCNNNNNNNGC	7 cut(s)	638, 946, 968, 995, 1283, 1598, 1709
BstNI	CCWGG	5 cut(s)	417, 840, 1584, 1860, 1881
BstNSI	RCATGY	2 cut(s)	569, 1571
BstSCI	CCNGG	5 cut(s)	415, 838, 1582, 1858, 1879
BstSFI	CTRYAG	2 cut(s)	147, 1704
BstSLI	GKGCMC	2 cut(s)	1594, 2074
BstV1I	GCAGC	5 cut(s)	625, 933, 946, 1679, 1712
BstV2I	GAAGAC	1 cut(s)	108
BstX2I	RGATCY	2 cut(s)	1935, 2032
BstXI	CCANNNNNNTGG	1 cut(s)	1901
BstYI	RGATCY	2 cut(s)	1935, 2032
BsuI	GTATCC	2 cut(s)	1590, 1813
BsuRI	GGCC	5 cut(s)	175, 905, 1059, 1639, 1884
BtsCI	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BtsI	GCAGTG	1 cut(s)	873
BtsIMutI	CAGTG	2 cut(s)	873, 1960
Cac8I	GCNNGC	3 cut(s)	491, 567, 1057
CaiI	CAGNNNCTG	1 cut(s)	962
CfoI	GCGC	2 cut(s)	856, 1073
Cfr13I	GGNCC	4 cut(s)	26, 344, 1057, 1352
Csp6I	GTAC	8 cut(s)	429, 665, 671, 1004, 1086, 1164, 1520, 1691
CviAII	CATG	5 cut(s)	566, 888, 986, 1033, 1568
CviQI	GTAC	8 cut(s)	429, 665, 671, 1004, 1086, 1164, 1520, 1691
DdeI	CTNAG	6 cut(s)	705, 776, 864, 1204, 1483, 1872
DpnI	GATC	6 cut(s)	186, 510, 795, 1644, 1937, 2034
DpnII	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
DraI	TTTAAA	1 cut(s)	846
EaeI	YGGCCR	1 cut(s)	903
Eam1104I	CTCTTC	6 cut(s)	468, 725, 901, 933, 1472, 1926
EarI	CTCTTC	6 cut(s)	468, 725, 901, 933, 1472, 1926
Ecl136II	GAGCTC	1 cut(s)	1482
Eco130I	CCWWGG	2 cut(s)	900, 917
Eco147I	AGGCCT	1 cut(s)	175
Eco24I	GRGCYC	1 cut(s)	1484
Eco31I	GGTCTC	1 cut(s)	708
Eco32I	GATATC	1 cut(s)	1538
Eco47I	GGWCC	3 cut(s)	26, 344, 1352
Eco53kI	GAGCTC	1 cut(s)	1482
Eco57I	CTGAAG	5 cut(s)	102, 449, 765, 962, 1020
Eco72I	CACGTG	1 cut(s)	1595
Eco88I	CYCGRG	1 cut(s)	560
EcoICRI	GAGCTC	1 cut(s)	1482
EcoO109I	RGGNCCY	2 cut(s)	26, 1352
EcoRI	GAATTC	1 cut(s)	1232
EcoRII	CCWGG	5 cut(s)	415, 838, 1582, 1858, 1879
EcoRV	GATATC	1 cut(s)	1538
EcoT14I	CCWWGG	2 cut(s)	900, 917
EcoT22I	ATGCAT	1 cut(s)	106
EcoT38I	GRGCYC	1 cut(s)	1484
ErhI	CCWWGG	2 cut(s)	900, 917
Esp3I	CGTCTC	1 cut(s)	986
FaeI	CATG	5 cut(s)	569, 891, 989, 1036, 1571
FalI	AAGNNNNNCTT	2 cut(s)	160, 192
FaqI	GGGAC	3 cut(s)	39, 298, 453
FatI	CATG	5 cut(s)	565, 887, 985, 1032, 1567
FauI	CCCGC	2 cut(s)	1048, 2016
FbaI	TGATCA	2 cut(s)	184, 1642
Fnu4HI	GCNGC	5 cut(s)	639, 947, 960, 1668, 1701
FokI	GGATG	6 cut(s)	113, 284, 437, 630, 676, 1443
FriOI	GRGCYC	1 cut(s)	1484
Fsp4HI	GCNGC	5 cut(s)	639, 947, 960, 1668, 1701
FspBI	CTAG	4 cut(s)	674, 1017, 1041, 1221
FspI	TGCGCA	1 cut(s)	855
GlaI	GCGC	2 cut(s)	855, 1072
GluI	GCNGC	5 cut(s)	639, 947, 960, 1668, 1701
GsaI	CCCAGC	2 cut(s)	439, 686
GsuI	CTGGAG	2 cut(s)	381, 822
HaeIII	GGCC	5 cut(s)	175, 905, 1059, 1639, 1884
HhaI	GCGC	2 cut(s)	856, 1073
Hin1II	CATG	5 cut(s)	569, 891, 989, 1036, 1571
Hin6I	GCGC	2 cut(s)	854, 1071
HinP1I	GCGC	2 cut(s)	854, 1071
HincII	GTYRAC	3 cut(s)	505, 679, 1149
HindII	GTYRAC	3 cut(s)	505, 679, 1149
HinfI	GANTC	3 cut(s)	122, 441, 605
HpaI	GTTAAC	1 cut(s)	505
HphI	GGTGA	5 cut(s)	544, 803, 826, 1333, 1628
Hpy166II	GTNNAC	5 cut(s)	505, 679, 817, 1149, 1592
Hpy188I	TCNGA	9 cut(s)	52, 121, 468, 867, 1207, 1262, 1486, 1661, 1987
Hpy188III	TCNNGA	6 cut(s)	98, 248, 1046, 1460, 1939, 2017
Hpy8I	GTNNAC	5 cut(s)	505, 679, 817, 1149, 1592
Hpy99I	CGWCG	1 cut(s)	1182
HpyAV	CCTTC	5 cut(s)	382, 463, 518, 1343, 1920
HpyCH4IV	ACGT	4 cut(s)	643, 1180, 1594, 1672
HpyF10VI	GCNNNNNNNGC	7 cut(s)	638, 946, 968, 995, 1283, 1598, 1709
HpyF3I	CTNAG	6 cut(s)	705, 776, 864, 1204, 1483, 1872
HpySE526I	ACGT	4 cut(s)	643, 1180, 1594, 1672
Hsp92II	CATG	5 cut(s)	569, 891, 989, 1036, 1571
HspAI	GCGC	2 cut(s)	854, 1071
Ksp22I	TGATCA	2 cut(s)	184, 1642
KspAI	GTTAAC	1 cut(s)	505
Kzo9I	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
LguI	GCTCTTC	2 cut(s)	468, 1472
LmnI	GCTCC	4 cut(s)	140, 1068, 1679, 1709
Lsp1109I	GCAGC	5 cut(s)	625, 933, 946, 1679, 1712
LweI	GCATC	6 cut(s)	91, 113, 301, 460, 476, 608
MaeI	CTAG	4 cut(s)	674, 1017, 1041, 1221
MaeII	ACGT	4 cut(s)	643, 1180, 1594, 1672
MaeIII	GTNAC	5 cut(s)	289, 1019, 1094, 1266, 1525
MalI	GATC	6 cut(s)	186, 510, 795, 1644, 1937, 2034
MboI	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
MfeI	CAATTG	1 cut(s)	1686
MflI	RGATCY	2 cut(s)	1935, 2032
MhlI	GDGCHC	3 cut(s)	1484, 1594, 2074
MlyI	GAGTC	1 cut(s)	435
MmeI	TCCRAC	1 cut(s)	1240
Mph1103I	ATGCAT	1 cut(s)	106
MroXI	GAANNNNTTC	1 cut(s)	927
MspA1I	CMGCKG	2 cut(s)	959, 1703
MspR9I	CCNGG	5 cut(s)	417, 840, 1584, 1860, 1881
MunI	CAATTG	1 cut(s)	1686
MvaI	CCWGG	5 cut(s)	417, 840, 1584, 1860, 1881
MwoI	GCNNNNNNNGC	7 cut(s)	638, 946, 968, 995, 1283, 1598, 1709
NdeII	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
NlaIII	CATG	5 cut(s)	569, 891, 989, 1036, 1571
NlaIV	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
NmuCI	GTSAC	3 cut(s)	289, 1019, 1094
NsbI	TGCGCA	1 cut(s)	855
NsiI	ATGCAT	1 cut(s)	106
NspI	RCATGY	2 cut(s)	569, 1571
PaeI	GCATGC	1 cut(s)	569
PaeR7I	CTCGAG	1 cut(s)	560
PceI	AGGCCT	1 cut(s)	175
PciI	ACATGT	1 cut(s)	1567
PciSI	GCTCTTC	2 cut(s)	468, 1472
PdmI	GAANNNNTTC	1 cut(s)	927
PfeI	GAWTC	2 cut(s)	122, 605
PflMI	CCANNNNNTGG	1 cut(s)	1109
PfoI	TCCNGGA	1 cut(s)	1858
PkrI	GCNGC	5 cut(s)	640, 948, 961, 1669, 1702
Ple19I	CGATCG	1 cut(s)	511
PleI	GAGTC	1 cut(s)	435
PmaCI	CACGTG	1 cut(s)	1595
PmlI	CACGTG	1 cut(s)	1595
PpsI	GAGTC	1 cut(s)	435
Ppu21I	YACGTR	2 cut(s)	644, 1595
PpuMI	RGGWCCY	2 cut(s)	26, 1352
PscI	ACATGT	1 cut(s)	1567
Psp124BI	GAGCTC	1 cut(s)	1484
Psp5II	RGGWCCY	2 cut(s)	26, 1352
Psp6I	CCWGG	5 cut(s)	415, 838, 1582, 1858, 1879
PspCI	CACGTG	1 cut(s)	1595
PspFI	CCCAGC	2 cut(s)	435, 682
PspGI	CCWGG	5 cut(s)	415, 838, 1582, 1858, 1879
PspN4I	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
PspPI	GGNCC	4 cut(s)	26, 344, 1057, 1352
PspPPI	RGGWCCY	2 cut(s)	26, 1352
PspXI	VCTCGAGB	1 cut(s)	560
PstNI	CAGNNNCTG	1 cut(s)	962
PsuI	RGATCY	2 cut(s)	1935, 2032
PvuI	CGATCG	1 cut(s)	511
PvuII	CAGCTG	2 cut(s)	959, 1703
RsaI	GTAC	8 cut(s)	430, 666, 672, 1005, 1087, 1165, 1521, 1692
RsaNI	GTAC	8 cut(s)	429, 665, 671, 1004, 1086, 1164, 1520, 1691
SacI	GAGCTC	1 cut(s)	1484
SapI	GCTCTTC	2 cut(s)	468, 1472
SatI	GCNGC	5 cut(s)	639, 947, 960, 1668, 1701
Sau3AI	GATC	6 cut(s)	184, 508, 793, 1642, 1935, 2032
Sau96I	GGNCC	4 cut(s)	26, 344, 1057, 1352
ScaI	AGTACT	1 cut(s)	1005
SchI	GAGTC	1 cut(s)	435
ScrFI	CCNGG	5 cut(s)	417, 840, 1584, 1860, 1881
SduI	GDGCHC	3 cut(s)	1484, 1594, 2074
SfaNI	GCATC	6 cut(s)	91, 113, 301, 460, 476, 608
SfcI	CTRYAG	2 cut(s)	147, 1704
Sfr274I	CTCGAG	1 cut(s)	560
SinI	GGWCC	3 cut(s)	26, 344, 1352
SlaI	CTCGAG	1 cut(s)	560
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SpeI	ACTAGT	1 cut(s)	673
SphI	GCATGC	1 cut(s)	569
SseBI	AGGCCT	1 cut(s)	175
SsiI	CCGC	2 cut(s)	1055, 2023
SspI	AATATT	1 cut(s)	2047
SspMI	CTAG	4 cut(s)	674, 1017, 1041, 1221
SstI	GAGCTC	1 cut(s)	1484
StuI	AGGCCT	1 cut(s)	175
StyD4I	CCNGG	5 cut(s)	415, 838, 1582, 1858, 1879
StyI	CCWWGG	2 cut(s)	900, 917
TaiI	ACGT	4 cut(s)	646, 1183, 1597, 1675
TaqI	TCGA	8 cut(s)	309, 484, 511, 561, 1177, 1184, 1725, 1737
TatI	WGTACW	2 cut(s)	1003, 1085
TfiI	GAWTC	2 cut(s)	122, 605
TscAI	CASTG	2 cut(s)	880, 1960
TseFI	GTSAC	3 cut(s)	289, 1019, 1094
TseI	GCWGC	5 cut(s)	638, 946, 959, 1667, 1700
Tsp45I	GTSAC	3 cut(s)	289, 1019, 1094
TspDTI	ATGAA	7 cut(s)	561, 1065, 1266, 1362, 1545, 1771, 2106
TspRI	CASTG	2 cut(s)	880, 1960
Van91I	CCANNNNNTGG	1 cut(s)	1109
VneI	GTGCAC	1 cut(s)	1590
VpaK11BI	GGWCC	3 cut(s)	26, 344, 1352
XapI	RAATTY	4 cut(s)	973, 1232, 1415, 1652
XceI	RCATGY	2 cut(s)	569, 1571
XhoI	CTCGAG	1 cut(s)	560
XmnI	GAANNNNTTC	1 cut(s)	927
XspI	CTAG	4 cut(s)	674, 1017, 1041, 1221
ZrmI	AGTACT	1 cut(s)	1005
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.