Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0004945

Physical Location & Seq

Genomic Coordinates Forward (+)

2183349 .. 2191630

Gene Structure 8282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g04000.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2115 bp

ATGACATCAGTTATAGGTTTTGGGGGACCTTACTTTTTTGGGTTGAGTTCCGAGAGTAAGCCTCGGTTTAGAGTGCAAGGTAGAAGTTTAAGTAATCAGGATGCATCAGTTTTGTCTTCAGACTCAATTGTAGTGAATGGAGCTTCTGTAGTTGGGGAGAGGGAGGGAAGTAGGCCTTGGGTTGATCATGGGAATGGCAGTTTGAGGCATAAGGTTAAGGACAAACAGAACATAGAAGATGGTGTACTTGAAAGTTTGGAACCTTTGTGGGATGACGGGTACGGGACTGTGACTGTAAAGGATTATTTCGATGCAAGTGAGGAGATGATTAAGCCTGATGGTGGTCCTCCCCGATGGTTTAGCCCCCTTGCTCGCAACCCTCCATTGAAGGATTCTCCAGTTCTTTTGTTTTTGCCTGGGTTGGATGGTACTGGGTGGGGACTCATTTTGCATCATAAAGCTCTTCAGAAGGTCTTTGAAGTTCGATGCTTGCATATTCCAGTTAATGATCGAACACCATTTGAAGGTTTGGTGAAACTGGTGGAAGACACTATTAGGCTTGAGCATGCTTCATCTCCCAACAAGCCAATTTATTTGGTTGGAGATTCTTTTGGAGGATGCTTATCACTTGCTGTTGCTGCACGCAATCCTACCATTGATCTAGTACTGATACTAGTCAACCCAGCAACATCCATGGAGAGGTCTCAATTACTACCTCTTTTCCCAATCTTGGAGGCTTTGCCTGATGAATTACATATCGGAGTTCCCTATCTTCTCAGTTTTGTTATGGGTGATCCAATGAAAATGGCGATGGTTAACATCAACAGTAGGCTTCCTCCCAGTTTAAAATTCATGCAACTGTCTCAGAATCTCACTGCTTTGCTACCATATCTCTCTTCTTTGGCCGATATTATACCAAAAGAAACTCTTCTGTGGAAGCTGAAGTTGCTCAGATCAGCTTCTGGTTATGCAAATTCCCGTCTGCATGCTGTTAAAGCTGAAGTACTTGTGCTGGCTAGTGACAAGGATAACATGGTACCTAGTCAAGATGAAGCGGAACGGCTAAGGAGCTCATTACAGAACTGTACAGTTCGTCACTTCCCGGACAATGGGCATACCCTTTTACTGGAAGATGGGGTTAATTTGTTGACTGTTATAAAACGTACTTTCAAATTTCGCCGGACGAGGAAGCATGATAGTTTCTCAGATTTCCTTCCTCCTAGTATGACAGAATTTAACTATACATTTGATCAAGAAGTCGGATTGTTACGCTTTGCTGCTGGTTCTGTTCTTTTCTCAACATTGGAAGATGGAACAGTAGTGAGAGGTCTTGCAGGAGTTCCAAATGAAGGACCTGTGTTAATTGTTGGCTATCACAATTTAATGGGGCTGGAACTGGTTTCTTTAGTTGAAGAATTTTTAAGAGAGAAGAAAATAATGTTTCGTGGTTTAGCGCATCCAGAACTGTTGTTGGGGAAGAGCTCAAGTCATTTTGACATTCTCAAATTATTTGGTGCATTACCTGTTACGCCAAGAAATCTATTCAAAGCACTTTCATCAAAATCACATGTTCTCCTGTATCCTGGTGGTGCACGTGAAGCTCTCCATTACAAGGGTGAAGAATACAAGTTATTCTGGCCTGATCAACCTGAATTTGTCAGAATGGCTGCACGTTTTGGGGCCACAATTGTACCATTTGCAGCTGTAGGAGAAGATGACTTACTGGAATTGGTTCTCGACTACAATGACATAAAGAATATCCCTGTGGTGGGTGACTATTTCATCAATGCTAATAATGATGCTGTAAATTTGAGGGAGGATACAAGTGGAGAAGTTGCCAATCAAAACCTCTTTATTCCGGGAGTTCTGCCTAAGTTGCCAGGCCGTTATTACTATTTGTTTGGGAAACCAATAGAAACAAAAGGAAGGGAAGAGATCCTGAAAGACAAAGACAGTGCAAACAAATTGTATTTGGAGATACAATCTGAAATTGAAAAAAGTTTAGCATACTTAATCAAGAAGCGGGAGGAGGATCCTTACAGGAATATTATTGACAGAACAGTATATCGGGCACTGCATTCCCCTCTAAATGAAGTTCCAACATTTGAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGAT Hydrolase_4

Protein Analysis

705

Amino Acids

78.57

Weight (kDa)

5.93

Isoelectric Point (pI)

46.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	135 - 378	1.5e-06	Alpha/beta hydrolase family
Hydrolase_4	PF12146	181 - 377	9e-10	Serine aminopeptidase, S33
DAGAT	PF03982	501 - 578	1.5e-06	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54570
fragaria_vesca	FvH4_6g03990 FvH4_6g04000
malus_domestica	MD04G1216900.v1.1 MD12G1233500.v1.1
prunus_persica	Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1
pyrus_communis	pycom04g19300 pycom12g21610
rosa_chinensis	RchiOBHm_Chr3g0452581
rosa_laevigata	RLG00000014771 RLG00000025582
rosa_multiflora	Rmu_co8485569.1_g000001 Rmu_sc0004414.1_g000004 Rmu_sc0028651.1_g000001 Rmu_sc0032164.1_g000001
rosa_roxburghii	Rroxscaffold_6G00429620 Rroxscaffold_7G00209280 Rroxscaffold_7G00209300
rosa_rugosa	Rorug02G0641400 Rorug02G0641500 Rorug02G0641500
rosa_samantha	Rh3AG044000 Rh3BG046100 Rh3CG044800 Rh3DG045100
rosa_wichuraiana	Rw3G003410 Rw6G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1157
Acc65I	GGTACC	1 cut(s)	1036
AccB1I	GGYRCC	1 cut(s)	1036
AciI	CCGC	2 cut(s)	1055, 2023
AclWI	GGATC	4 cut(s)	788, 1930, 2027, 2040
AcoI	YGGCCR	1 cut(s)	903
AcsI	RAATTY	7 cut(s)	848, 973, 1172, 1232, 1415, 1652, 1807
AcuI	CTGAAG	4 cut(s)	102, 449, 962, 1020
AcvI	CACGTG	1 cut(s)	1595
AfaI	GTAC	9 cut(s)	246, 281, 430, 666, 1005, 1038, 1087, 1165, 1692
AflIII	ACRYGT	1 cut(s)	1567
AgsI	TTSAA	8 cut(s)	251, 388, 479, 524, 1171, 1412, 1546, 1994
AhlI	ACTAGT	1 cut(s)	673
AjnI	CCWGG	3 cut(s)	415, 1582, 1879
AjuI	GAANNNNNNNTTGG	2 cut(s)	160, 192
AluBI	AGCT	9 cut(s)	143, 461, 940, 959, 998, 1071, 1482, 1601, 1703
AluI	AGCT	9 cut(s)	143, 461, 940, 959, 998, 1071, 1482, 1601, 1703
Alw21I	GWGCWC	3 cut(s)	1073, 1484, 1594
Alw26I	GTCTC	2 cut(s)	708, 867
Alw44I	GTGCAC	1 cut(s)	1590
AlwI	GGATC	4 cut(s)	788, 1930, 2027, 2040
AlwNI	CAGNNNCTG	1 cut(s)	962
AoxI	GGCC	5 cut(s)	173, 903, 1637, 1680, 1882
ApaLI	GTGCAC	1 cut(s)	1590
ApeKI	GCWGC	4 cut(s)	638, 1277, 1667, 1700
ApoI	RAATTY	7 cut(s)	848, 973, 1172, 1232, 1415, 1652, 1807
Asp700I	GAANNNNTTC	2 cut(s)	927, 1731
Asp718I	GGTACC	1 cut(s)	1036
AspLEI	GCGC	1 cut(s)	1456
AspS9I	GGNCC	4 cut(s)	26, 344, 1352, 1680
AsuC2I	CCSGG	2 cut(s)	1103, 1860
AsuHPI	GGTGA	4 cut(s)	544, 803, 1628, 1784
AvaII	GGWCC	3 cut(s)	26, 344, 1352
BaeGI	GKGCMC	2 cut(s)	1594, 2074
BaeI	ACNNNNGTAYC	2 cut(s)	1674, 1707
BamHI	GGATCC	1 cut(s)	2032
BanI	GGYRCC	1 cut(s)	1036
BanII	GRGCYC	2 cut(s)	1073, 1484
BarI	GAAGNNNNNNTAC	4 cut(s)	228, 260, 1704, 1736
BbrPI	CACGTG	1 cut(s)	1595
BbsI	GAAGAC	2 cut(s)	108, 552
Bbv12I	GWGCWC	3 cut(s)	1073, 1484, 1594
BbvI	GCAGC	4 cut(s)	625, 1264, 1654, 1712
BccI	CCATC	7 cut(s)	233, 332, 348, 419, 805, 1127, 1304
BceAI	ACGGC	2 cut(s)	1076, 1869
BciT130I	CCWGG	3 cut(s)	417, 1584, 1881
BciVI	GTATCC	2 cut(s)	1590, 1813
BclI	TGATCA	3 cut(s)	184, 1249, 1642
BcnI	CCSGG	2 cut(s)	1103, 1860
BcoDI	GTCTC	2 cut(s)	708, 867
BcuI	ACTAGT	1 cut(s)	673
BfaI	CTAG	5 cut(s)	662, 674, 1017, 1041, 1221
BfmI	CTRYAG	2 cut(s)	147, 1704
BfuI	GTATCC	2 cut(s)	1590, 1813
BisI	GCNGC	4 cut(s)	639, 1278, 1668, 1701
BlsI	GCNGC	4 cut(s)	640, 1279, 1669, 1702
BmcAI	AGTACT	2 cut(s)	666, 1005
Bme1390I	CCNGG	5 cut(s)	417, 1103, 1584, 1860, 1881
Bme18I	GGWCC	3 cut(s)	26, 344, 1352
BmgT120I	GGNCC	4 cut(s)	26, 344, 1352, 1680
BmiI	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
BmrFI	CCNGG	5 cut(s)	417, 1103, 1584, 1860, 1881
BmrI	ACTGGG	2 cut(s)	441, 834
BmsI	GCATC	8 cut(s)	91, 113, 301, 460, 476, 608, 1465, 1789
BmuI	ACTGGG	2 cut(s)	441, 834
BpiI	GAAGAC	2 cut(s)	108, 552
BplI	GAGNNNNNCTC	2 cut(s)	46, 78
BpmI	CTGGAG	1 cut(s)	381
Bpu10I	CCTNAGC	1 cut(s)	1064
BpuEI	CTTGAG	2 cut(s)	581, 1468
BpuMI	CCSGG	2 cut(s)	1103, 1860
BsaAI	YACGTR	1 cut(s)	1595
BsaBI	GATNNNNATC	1 cut(s)	622
BsaI	GGTCTC	1 cut(s)	708
BsaJI	CCNNGG	4 cut(s)	62, 176, 416, 693
BsaXI	ACNNNNNCTCC	4 cut(s)	132, 162, 1557, 1587
Bse1I	ACTGG	8 cut(s)	398, 436, 500, 543, 840, 1131, 1401, 1728
Bse8I	GATNNNNATC	1 cut(s)	622
BseBI	CCWGG	3 cut(s)	417, 1584, 1881
BseDI	CCNNGG	4 cut(s)	62, 176, 416, 693
BseGI	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BseJI	GATNNNNATC	1 cut(s)	622
BseMII	CTCAG	4 cut(s)	790, 878, 964, 1218
BseNI	ACTGG	8 cut(s)	398, 436, 500, 543, 840, 1131, 1401, 1728
BseRI	GAGGAG	2 cut(s)	335, 2042
BseSI	GKGCMC	2 cut(s)	1594, 2074
BseXI	GCAGC	4 cut(s)	625, 1264, 1654, 1712
BseYI	CCCAGC	1 cut(s)	682
BsgI	GTGCAG	2 cut(s)	624, 1653
BshFI	GGCC	5 cut(s)	175, 905, 1639, 1682, 1884
BshNI	GGYRCC	1 cut(s)	1036
BsiHKAI	GWGCWC	3 cut(s)	1073, 1484, 1594
BsiSI	CCGG	3 cut(s)	1103, 1180, 1859
BslFI	GGGAC	3 cut(s)	39, 298, 453
BsmAI	GTCTC	2 cut(s)	708, 867
BsmFI	GGGAC	3 cut(s)	39, 298, 453
BsmI	GAATGC	1 cut(s)	2077
BsnI	GGCC	5 cut(s)	175, 905, 1639, 1682, 1884
Bso31I	GGTCTC	1 cut(s)	708
Bsp1286I	GDGCHC	4 cut(s)	1073, 1484, 1594, 2074
Bsp1407I	TGTACA	1 cut(s)	1085
Bsp143I	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
Bsp19I	CCATGG	1 cut(s)	693
BspACI	CCGC	2 cut(s)	1055, 2023
BspANI	GGCC	5 cut(s)	175, 905, 1639, 1682, 1884
BspCNI	CTCAG	4 cut(s)	789, 877, 963, 1217
BspLI	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
BspPI	GGATC	4 cut(s)	788, 1930, 2027, 2040
BspQI	GCTCTTC	2 cut(s)	468, 1472
BspT107I	GGYRCC	1 cut(s)	1036
BspTNI	GGTCTC	1 cut(s)	708
BsrGI	TGTACA	1 cut(s)	1085
BsrI	ACTGG	8 cut(s)	398, 436, 500, 543, 840, 1131, 1401, 1728
BssECI	CCNNGG	4 cut(s)	62, 176, 416, 693
BssMI	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
BssT1I	CCWWGG	2 cut(s)	176, 693
Bst2UI	CCWGG	3 cut(s)	417, 1584, 1881
Bst6I	CTCTTC	5 cut(s)	468, 901, 933, 1472, 1926
BstAUI	TGTACA	1 cut(s)	1085
BstBAI	YACGTR	1 cut(s)	1595
BstC8I	GCNNGC	6 cut(s)	373, 491, 567, 643, 987, 1014
BstDEI	CTNAG	6 cut(s)	776, 864, 950, 1064, 1204, 1872
BstDSI	CCRYGG	1 cut(s)	693
BstF5I	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BstHHI	GCGC	1 cut(s)	1456
BstKTI	GATC	9 cut(s)	187, 511, 661, 796, 956, 1252, 1645, 1938, 2035
BstMAI	GTCTC	2 cut(s)	708, 867
BstMBI	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
BstMWI	GCNNNNNNNGC	5 cut(s)	638, 946, 995, 1598, 1876
BstNI	CCWGG	3 cut(s)	417, 1584, 1881
BstNSI	RCATGY	3 cut(s)	569, 989, 1571
BstSCI	CCNGG	5 cut(s)	415, 1101, 1582, 1858, 1879
BstSFI	CTRYAG	2 cut(s)	147, 1704
BstSLI	GKGCMC	2 cut(s)	1594, 2074
BstV1I	GCAGC	4 cut(s)	625, 1264, 1654, 1712
BstV2I	GAAGAC	2 cut(s)	108, 552
BstX2I	RGATCY	2 cut(s)	1935, 2032
BstYI	RGATCY	2 cut(s)	1935, 2032
BsuI	GTATCC	2 cut(s)	1590, 1813
BsuRI	GGCC	5 cut(s)	175, 905, 1639, 1682, 1884
BtgI	CCRYGG	1 cut(s)	693
BtgZI	GCGATG	1 cut(s)	824
BtsCI	GGATG	6 cut(s)	106, 277, 430, 623, 689, 1456
BtsI	GCAGTG	2 cut(s)	873, 2072
BtsIMutI	CAGTG	3 cut(s)	873, 1960, 2072
Cac8I	GCNNGC	6 cut(s)	373, 491, 567, 643, 987, 1014
CaiI	CAGNNNCTG	1 cut(s)	962
CfoI	GCGC	1 cut(s)	1456
Cfr13I	GGNCC	4 cut(s)	26, 344, 1352, 1680
Csp6I	GTAC	9 cut(s)	245, 280, 429, 665, 1004, 1037, 1086, 1164, 1691
CviAII	CATG	8 cut(s)	188, 566, 694, 853, 986, 1033, 1193, 1568
CviQI	GTAC	9 cut(s)	245, 280, 429, 665, 1004, 1037, 1086, 1164, 1691
DdeI	CTNAG	6 cut(s)	776, 864, 950, 1064, 1204, 1872
DpnI	GATC	9 cut(s)	186, 510, 660, 795, 955, 1251, 1644, 1937, 2034
DpnII	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
DraI	TTTAAA	1 cut(s)	846
EaeI	YGGCCR	1 cut(s)	903
Eam1104I	CTCTTC	5 cut(s)	468, 901, 933, 1472, 1926
EarI	CTCTTC	5 cut(s)	468, 901, 933, 1472, 1926
Ecl136II	GAGCTC	2 cut(s)	1071, 1482
Eco130I	CCWWGG	2 cut(s)	176, 693
Eco147I	AGGCCT	1 cut(s)	175
Eco24I	GRGCYC	2 cut(s)	1073, 1484
Eco31I	GGTCTC	1 cut(s)	708
Eco47I	GGWCC	3 cut(s)	26, 344, 1352
Eco53kI	GAGCTC	2 cut(s)	1071, 1482
Eco57I	CTGAAG	4 cut(s)	102, 449, 962, 1020
Eco72I	CACGTG	1 cut(s)	1595
EcoICRI	GAGCTC	2 cut(s)	1071, 1482
EcoO109I	RGGNCCY	2 cut(s)	26, 1352
EcoRII	CCWGG	3 cut(s)	415, 1582, 1879
EcoT14I	CCWWGG	2 cut(s)	176, 693
EcoT22I	ATGCAT	1 cut(s)	106
EcoT38I	GRGCYC	2 cut(s)	1073, 1484
ErhI	CCWWGG	2 cut(s)	176, 693
FaeI	CATG	8 cut(s)	191, 569, 697, 856, 989, 1036, 1196, 1571
FalI	AAGNNNNNCTT	4 cut(s)	160, 192, 912, 944
FaqI	GGGAC	3 cut(s)	39, 298, 453
FatI	CATG	8 cut(s)	187, 565, 693, 852, 985, 1032, 1192, 1567
FauI	CCCGC	1 cut(s)	2016
FbaI	TGATCA	3 cut(s)	184, 1249, 1642
Fnu4HI	GCNGC	4 cut(s)	639, 1278, 1668, 1701
FokI	GGATG	6 cut(s)	113, 284, 437, 630, 676, 1443
FriOI	GRGCYC	2 cut(s)	1073, 1484
Fsp4HI	GCNGC	4 cut(s)	639, 1278, 1668, 1701
FspBI	CTAG	5 cut(s)	662, 674, 1017, 1041, 1221
GlaI	GCGC	1 cut(s)	1455
GluI	GCNGC	4 cut(s)	639, 1278, 1668, 1701
GsaI	CCCAGC	1 cut(s)	686
GsuI	CTGGAG	1 cut(s)	381
HaeIII	GGCC	5 cut(s)	175, 905, 1639, 1682, 1884
HapII	CCGG	3 cut(s)	1103, 1180, 1859
HhaI	GCGC	1 cut(s)	1456
Hin1II	CATG	8 cut(s)	191, 569, 697, 856, 989, 1036, 1196, 1571
Hin6I	GCGC	1 cut(s)	1454
HinP1I	GCGC	1 cut(s)	1454
HincII	GTYRAC	3 cut(s)	679, 817, 1149
HindII	GTYRAC	3 cut(s)	679, 817, 1149
HinfI	GANTC	5 cut(s)	122, 392, 441, 605, 868
HpaI	GTTAAC	1 cut(s)	817
HpaII	CCGG	3 cut(s)	1103, 1180, 1859
HphI	GGTGA	4 cut(s)	544, 803, 1628, 1784
Hpy166II	GTNNAC	5 cut(s)	245, 679, 817, 1149, 1592
Hpy188III	TCNNGA	7 cut(s)	98, 1046, 1253, 1460, 1736, 1939, 2017
Hpy8I	GTNNAC	5 cut(s)	245, 679, 817, 1149, 1592
HpyAV	CCTTC	6 cut(s)	382, 463, 518, 1223, 1343, 1920
HpyCH4IV	ACGT	3 cut(s)	1162, 1594, 1672
HpyF10VI	GCNNNNNNNGC	5 cut(s)	638, 946, 995, 1598, 1876
HpyF3I	CTNAG	6 cut(s)	776, 864, 950, 1064, 1204, 1872
HpySE526I	ACGT	3 cut(s)	1162, 1594, 1672
Hsp92II	CATG	8 cut(s)	191, 569, 697, 856, 989, 1036, 1196, 1571
HspAI	GCGC	1 cut(s)	1454
KpnI	GGTACC	1 cut(s)	1040
Ksp22I	TGATCA	3 cut(s)	184, 1249, 1642
KspAI	GTTAAC	1 cut(s)	817
Kzo9I	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
LguI	GCTCTTC	2 cut(s)	468, 1472
LmnI	GCTCC	2 cut(s)	140, 1068
Lsp1109I	GCAGC	4 cut(s)	625, 1264, 1654, 1712
LweI	GCATC	8 cut(s)	91, 113, 301, 460, 476, 608, 1465, 1789
MaeI	CTAG	5 cut(s)	662, 674, 1017, 1041, 1221
MaeII	ACGT	3 cut(s)	1162, 1594, 1672
MaeIII	GTNAC	6 cut(s)	289, 1019, 1094, 1266, 1525, 1772
MalI	GATC	9 cut(s)	186, 510, 660, 795, 955, 1251, 1644, 1937, 2034
MboI	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
MfeI	CAATTG	2 cut(s)	126, 1686
MflI	RGATCY	2 cut(s)	1935, 2032
MhlI	GDGCHC	4 cut(s)	1073, 1484, 1594, 2074
MlyI	GAGTC	2 cut(s)	116, 435
MmeI	TCCRAC	3 cut(s)	402, 580, 1240
Mph1103I	ATGCAT	1 cut(s)	106
MroXI	GAANNNNTTC	2 cut(s)	927, 1731
MslI	CAYNNNNRTG	1 cut(s)	192
MspA1I	CMGCKG	1 cut(s)	1703
MspI	CCGG	3 cut(s)	1103, 1180, 1859
MspR9I	CCNGG	5 cut(s)	417, 1103, 1584, 1860, 1881
MunI	CAATTG	2 cut(s)	126, 1686
Mva1269I	GAATGC	1 cut(s)	2077
MvaI	CCWGG	3 cut(s)	417, 1584, 1881
MwoI	GCNNNNNNNGC	5 cut(s)	638, 946, 995, 1598, 1876
NciI	CCSGG	2 cut(s)	1103, 1860
NcoI	CCATGG	1 cut(s)	693
NdeII	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
NlaIII	CATG	8 cut(s)	191, 569, 697, 856, 989, 1036, 1196, 1571
NlaIV	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
NmuCI	GTSAC	4 cut(s)	289, 1019, 1094, 1772
NsiI	ATGCAT	1 cut(s)	106
NspI	RCATGY	3 cut(s)	569, 989, 1571
PaeI	GCATGC	2 cut(s)	569, 989
PceI	AGGCCT	1 cut(s)	175
PciI	ACATGT	1 cut(s)	1567
PciSI	GCTCTTC	2 cut(s)	468, 1472
PctI	GAATGC	1 cut(s)	2077
PdmI	GAANNNNTTC	2 cut(s)	927, 1731
PfeI	GAWTC	3 cut(s)	392, 605, 868
PfoI	TCCNGGA	2 cut(s)	1101, 1858
PkrI	GCNGC	4 cut(s)	640, 1279, 1669, 1702
PleI	GAGTC	2 cut(s)	116, 435
PmaCI	CACGTG	1 cut(s)	1595
PmlI	CACGTG	1 cut(s)	1595
PpsI	GAGTC	2 cut(s)	116, 435
Ppu21I	YACGTR	1 cut(s)	1595
PpuMI	RGGWCCY	2 cut(s)	26, 1352
PscI	ACATGT	1 cut(s)	1567
PsiI	TTATAA	1 cut(s)	1157
Psp124BI	GAGCTC	2 cut(s)	1073, 1484
Psp5II	RGGWCCY	2 cut(s)	26, 1352
Psp6I	CCWGG	3 cut(s)	415, 1582, 1879
PspCI	CACGTG	1 cut(s)	1595
PspFI	CCCAGC	1 cut(s)	682
PspGI	CCWGG	3 cut(s)	415, 1582, 1879
PspN4I	GGNNCC	5 cut(s)	27, 261, 1038, 1681, 2034
PspPI	GGNCC	4 cut(s)	26, 344, 1352, 1680
PspPPI	RGGWCCY	2 cut(s)	26, 1352
PstNI	CAGNNNCTG	1 cut(s)	962
PsuI	RGATCY	2 cut(s)	1935, 2032
PvuII	CAGCTG	1 cut(s)	1703
RsaI	GTAC	9 cut(s)	246, 281, 430, 666, 1005, 1038, 1087, 1165, 1692
RsaNI	GTAC	9 cut(s)	245, 280, 429, 665, 1004, 1037, 1086, 1164, 1691
RseI	CAYNNNNRTG	1 cut(s)	192
SacI	GAGCTC	2 cut(s)	1073, 1484
SapI	GCTCTTC	2 cut(s)	468, 1472
SatI	GCNGC	4 cut(s)	639, 1278, 1668, 1701
Sau3AI	GATC	9 cut(s)	184, 508, 658, 793, 953, 1249, 1642, 1935, 2032
Sau96I	GGNCC	4 cut(s)	26, 344, 1352, 1680
ScaI	AGTACT	2 cut(s)	666, 1005
SchI	GAGTC	2 cut(s)	116, 435
ScrFI	CCNGG	5 cut(s)	417, 1103, 1584, 1860, 1881
SduI	GDGCHC	4 cut(s)	1073, 1484, 1594, 2074
SfaNI	GCATC	8 cut(s)	91, 113, 301, 460, 476, 608, 1465, 1789
SfcI	CTRYAG	2 cut(s)	147, 1704
SinI	GGWCC	3 cut(s)	26, 344, 1352
SmiMI	CAYNNNNRTG	1 cut(s)	192
SmlI	CTYRAG	2 cut(s)	560, 1483
SmoI	CTYRAG	2 cut(s)	560, 1483
SpeI	ACTAGT	1 cut(s)	673
SphI	GCATGC	2 cut(s)	569, 989
SseBI	AGGCCT	1 cut(s)	175
SsiI	CCGC	2 cut(s)	1055, 2023
SspI	AATATT	1 cut(s)	2047
SspMI	CTAG	5 cut(s)	662, 674, 1017, 1041, 1221
SstI	GAGCTC	2 cut(s)	1073, 1484
StuI	AGGCCT	1 cut(s)	175
StyD4I	CCNGG	5 cut(s)	415, 1101, 1582, 1858, 1879
StyI	CCWWGG	2 cut(s)	176, 693
TaiI	ACGT	3 cut(s)	1165, 1597, 1675
TaqI	TCGA	4 cut(s)	309, 484, 511, 1737
TatI	WGTACW	4 cut(s)	244, 664, 1003, 1085
TfiI	GAWTC	3 cut(s)	392, 605, 868
TscAI	CASTG	3 cut(s)	880, 1960, 2079
TseFI	GTSAC	4 cut(s)	289, 1019, 1094, 1772
TseI	GCWGC	4 cut(s)	638, 1277, 1667, 1700
Tsp45I	GTSAC	4 cut(s)	289, 1019, 1094, 1772
TspDTI	ATGAA	9 cut(s)	561, 762, 815, 841, 1065, 1362, 1545, 1771, 2106
TspRI	CASTG	3 cut(s)	880, 1960, 2079
VneI	GTGCAC	1 cut(s)	1590
VpaK11BI	GGWCC	3 cut(s)	26, 344, 1352
XapI	RAATTY	7 cut(s)	848, 973, 1172, 1232, 1415, 1652, 1807
XceI	RCATGY	3 cut(s)	569, 989, 1571
XmnI	GAANNNNTTC	2 cut(s)	927, 1731
XspI	CTAG	5 cut(s)	662, 674, 1017, 1041, 1221
ZrmI	AGTACT	2 cut(s)	666, 1005
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.