Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004945

Physical Location & Seq

Genomic Coordinates Reverse (-)

29947882 .. 29953829

Gene Structure 5948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1216900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGGCATCAGTTATAGGCTTTCCGGGTCCTCATTTTTTCGTGTTGAATTCGGAGAATAAGCTTCGGACTTGTGTACTGCAGGTTAAGAGTTTAGGTGGTAAGGATTCACCGGGGTTGTCATCCGAATCTGTTGTTTTGAATTGTGCTTCCGTAATCGAGGAGAGGGAGGAAAGGAGGTCTGTGATTGATAGAGGGAATGAGAGTTTGAGGCTTAAGGTTAAGGAGGAGAAGAGGGTGGAGGATGGTGGTGGTCTTGAAAGTTTGAAACCTTTGTACGATGATGGGTATGGGACTGTCACTGTAAAAGATTATTTTCATGCAAACAAGGAGATGATTAAGCCTGATGGTGGACCTCCCCGGTGGTTTTCTCCCATTGAGTGCGGGCGTCCACTGAAGGATTCTCCGATTCTTTTGTTTTTGCCTGGGCTGGATGGTACTGGATGGGGCCTCATTTTGCACCATAAAGCTCTTGGGAAGGCTTTTGAAGTTCGATGCCTGCATATTCCTGTTAATGATCGGACACCATTTGAAGGGCTGGTGAAATTTGTCGAAGAAACTATAAGGCTTGAGCATGCTTCATCTCCAAACAAGCCAATTTACTTGGTAGGAGATTCCTTTGGAGGATGCCTGTCACTTGCCGTTGCTGCACGTAATCCTACCATTGATTTAGTACTGACACTGGTCAATCCAGCTACATCGGTTGAAAGGTCTCCACTGCAACCTCTGTTCCCAATCTTGGCTGCTATGCCTGATGAATTACATATTGCTGTTCCCTATCTACTTAGCTTTGTTATGGGTGATCCAACAAAAATGGCGACGGTTAACATAGAAAATAGACTTCCTTCCAGTTTAAAGTTGACACAAATGTCACGCAATTTGACTGATTTGCTACCATATCTCTCTTCTTTGGCTGATATTATACCGAGGGACACTCTTCTTTGGAAGCTGAAGCTGCTCAAGTCAGCTGCTGCTTATGCAAATTCCCGTCTCCATGCTGTTAAAGCTGAAGTGCTAGTGTTGGCTAGTGGCAAAGATAACATGGTTCCAAGTCGAGATGAAGCAGAGCGACTGGCACGTTCATTACAGAATTGTACAGTACGACACTGCAATGACAATGGACATACTCTATTACTAGAAGATGGCGTTAATTTGATGTCGGTTATAAAAGGCACATACAAATACCGTCGTTCTAGGAAGCATGATTATGTCTCAGATTTTCTTCCTCCTAGTATGTGGGAATTCAAACATACATGTGATGAACAATTCGGGTTATTACGCCCATATACTGCTTCCGTAATGCTCTCAACATTGGAAGATGGGAAGATAGTGAGAGGTCTTGCTGGAGTTCCAGATGAAGGACCTGTCTTACTTGTTGGTAATCACAACATATTGGGATTGGAAGTTTATCCTTTAGTTGAAGAATTTTTAAGAGAGAAGAGCATAATCATTCGTGGAGTAGCACATCCAGCGTTGTTTTGGGGCAAGTACGGGAACTTGGCCCAATTTGACTTTCTCAAACTATTCGGCGCAGTACCTGTCGCAGCAAAAAACCTGTTCAAATTGCTTGCAATAAAATCTCATGTTCTGCTCTATCCTGGTGGTGCACGTGAGTCTCTCCATAACAAGGGTGAAGAATACAAGTTATTCTGGCCTGACCAACCCGAATTTGTGAGAATGGCTGCACACTTTGGGGCCACAATTGTACCATTTGCGGCTGTAGGAGAAGATGACATGCTAGAATTGGTTCTTGACCACAAGGACCTAATGAATATCCCAGTGGTGAGTAACTATCTCAAAGACGCTAATCTTGAATCTATAAAAATAAGGGATGAGTCGAGTGGGGAGGTTGCCAACGTACAACCCTTTTTTCCAGGGATTCTACCGAAGATACCAGGACGGTATTACTGTTTGTTTGGGAAGCCCATCGAAACAAAGGGAAAGAAAGAGATCCTGAAAGACAAAGAAAACGCAAAGGAATTGTACCTGCAGATACAATCAGACATTGAAAACAGTTTAGCGTACTTAATTAAAAAGCGGGAGGAGGATCCTTACAGGAATGTTTTCGACAGAACAATATATCGGGCAATGTATCACCATCTAGAGGAAGTTCCAACATTTGAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGAT Hydrolase_4

Protein Analysis

709

Amino Acids

79.2

Weight (kDa)

6.49

Isoelectric Point (pI)

49.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	175 - 380	1.3e-06	alpha/beta hydrolase fold
Hydrolase_4	PF12146	184 - 379	1e-09	Serine aminopeptidase, S33
DAGAT	PF03982	501 - 588	3.2e-07	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54570
fragaria_vesca	FvH4_6g03990 FvH4_6g04000
malus_domestica	MD04G1216900.v1.1 MD12G1233500.v1.1
prunus_persica	Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1
pyrus_communis	pycom04g19300 pycom12g21610
rosa_chinensis	RchiOBHm_Chr3g0452581
rosa_laevigata	RLG00000014771 RLG00000025582
rosa_multiflora	Rmu_co8485569.1_g000001 Rmu_sc0004414.1_g000004 Rmu_sc0028651.1_g000001 Rmu_sc0032164.1_g000001
rosa_roxburghii	Rroxscaffold_6G00429620 Rroxscaffold_7G00209280 Rroxscaffold_7G00209300
rosa_rugosa	Rorug02G0641400 Rorug02G0641500 Rorug02G0641500
rosa_samantha	Rh3AG044000 Rh3BG046100 Rh3CG044800 Rh3DG045100
rosa_wichuraiana	Rw3G003410 Rw6G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1163
AasI	GACNNNNNNGTC	1 cut(s)	865
Acc36I	ACCTGC	2 cut(s)	70, 1992
AciI	CCGC	3 cut(s)	381, 1712, 2035
AclWI	GGATC	4 cut(s)	794, 1942, 2039, 2052
AcsI	RAATTY	6 cut(s)	46, 542, 979, 1238, 1421, 1664
AcuI	CTGAAG	3 cut(s)	413, 968, 1026
AcvI	CACGTG	1 cut(s)	1607
AcyI	GRCGYC	1 cut(s)	385
AfiI	CCNNNNNNNGG	6 cut(s)	347, 530, 736, 1355, 1624, 2101
AflII	CTTAAG	1 cut(s)	212
AflIII	ACRYGT	1 cut(s)	1250
AjnI	CCWGG	4 cut(s)	421, 1594, 1870, 1891
AjuI	GAANNNNNNNTTGG	2 cut(s)	1494, 1526
AluBI	AGCT	8 cut(s)	61, 467, 692, 786, 946, 952, 965, 1004
AluI	AGCT	8 cut(s)	61, 467, 692, 786, 946, 952, 965, 1004
Alw21I	GWGCWC	1 cut(s)	1606
Alw26I	GTCTC	4 cut(s)	714, 992, 1213, 1617
Alw44I	GTGCAC	1 cut(s)	1602
AlwI	GGATC	4 cut(s)	794, 1942, 2039, 2052
AlwNI	CAGNNNCTG	2 cut(s)	968, 1535
AoxI	GGCC	4 cut(s)	445, 1497, 1649, 1692
ApaLI	GTGCAC	1 cut(s)	1602
ApeKI	GCWGC	7 cut(s)	644, 740, 952, 965, 968, 1541, 1679
ApoI	RAATTY	6 cut(s)	46, 542, 979, 1238, 1421, 1664
Asp700I	GAANNNNTTC	2 cut(s)	1743, 2060
AspLEI	GCGC	1 cut(s)	1529
AspS9I	GGNCC	7 cut(s)	26, 350, 445, 1358, 1498, 1692, 1759
AsuC2I	CCSGG	3 cut(s)	24, 111, 358
AsuHPI	GGTGA	6 cut(s)	99, 550, 809, 1640, 1792, 2084
AvaII	GGWCC	4 cut(s)	26, 350, 1358, 1759
BaeGI	GKGCMC	1 cut(s)	1606
BaeI	ACNNNNGTAYC	2 cut(s)	1686, 1719
BamHI	GGATCC	1 cut(s)	2044
BbrPI	CACGTG	1 cut(s)	1607
Bbv12I	GWGCWC	1 cut(s)	1606
BbvI	GCAGC	7 cut(s)	631, 727, 939, 952, 955, 1553, 1666
BccI	CCATC	9 cut(s)	236, 275, 338, 425, 435, 1133, 1310, 1931, 2103
BceAI	ACGGC	1 cut(s)	623
BciT130I	CCWGG	4 cut(s)	423, 1596, 1872, 1893
BcnI	CCSGG	3 cut(s)	24, 111, 358
BcoDI	GTCTC	4 cut(s)	714, 992, 1213, 1617
BfaI	CTAG	7 cut(s)	1013, 1023, 1133, 1191, 1227, 1736, 2099
BfmI	CTRYAG	3 cut(s)	77, 1716, 1985
BfrI	CTTAAG	1 cut(s)	212
BfuAI	ACCTGC	2 cut(s)	70, 1992
BisI	GCNGC	8 cut(s)	645, 741, 953, 966, 969, 1542, 1680, 1713
BlsI	GCNGC	8 cut(s)	646, 742, 954, 967, 970, 1543, 1681, 1714
BmcAI	AGTACT	1 cut(s)	672
Bme1390I	CCNGG	7 cut(s)	24, 111, 358, 423, 1596, 1872, 1893
Bme18I	GGWCC	4 cut(s)	26, 350, 1358, 1759
BmgT120I	GGNCC	7 cut(s)	26, 350, 445, 1358, 1498, 1692, 1759
BmiI	GGNNCC	5 cut(s)	27, 446, 1044, 1693, 2046
BmrFI	CCNGG	7 cut(s)	24, 111, 358, 423, 1596, 1872, 1893
BmrI	ACTGGG	1 cut(s)	1769
BmsI	GCATC	3 cut(s)	14, 482, 614
BmuI	ACTGGG	1 cut(s)	1769
BoxI	GACNNNNGTC	1 cut(s)	680
BplI	GAGNNNNNCTC	2 cut(s)	916, 948
BpmI	CTGGAG	1 cut(s)	1362
BpuEI	CTTGAG	2 cut(s)	587, 941
BpuMI	CCSGG	3 cut(s)	24, 111, 358
BsaAI	YACGTR	2 cut(s)	650, 1607
BsaHI	GRCGYC	1 cut(s)	385
BsaI	GGTCTC	1 cut(s)	714
BsaJI	CCNNGG	5 cut(s)	110, 356, 422, 923, 1871
Bsc4I	CCNNNNNNNGG	6 cut(s)	347, 530, 736, 1355, 1624, 2101
Bse1I	ACTGG	5 cut(s)	442, 684, 846, 1074, 1775
Bse3DI	GCAATG	2 cut(s)	1114, 2091
BseBI	CCWGG	4 cut(s)	423, 1596, 1872, 1893
BseDI	CCNNGG	5 cut(s)	110, 356, 422, 923, 1871
BseGI	GGATG	7 cut(s)	119, 247, 436, 446, 629, 1462, 1834
BseLI	CCNNNNNNNGG	6 cut(s)	347, 530, 736, 1355, 1624, 2101
BseMI	GCAATG	2 cut(s)	1114, 2091
BseMII	CTCAG	1 cut(s)	1224
BseNI	ACTGG	5 cut(s)	442, 684, 846, 1074, 1775
BseRI	GAGGAG	3 cut(s)	173, 239, 2054
BseSI	GKGCMC	1 cut(s)	1606
BseXI	GCAGC	7 cut(s)	631, 727, 939, 952, 955, 1553, 1666
BsgI	GTGCAG	2 cut(s)	630, 1665
BshFI	GGCC	4 cut(s)	447, 1499, 1651, 1694
BsiHKAI	GWGCWC	1 cut(s)	1606
BsiSI	CCGG	3 cut(s)	23, 110, 358
BslFI	GGGAC	2 cut(s)	304, 941
BslI	CCNNNNNNNGG	6 cut(s)	347, 530, 736, 1355, 1624, 2101
BsmAI	GTCTC	4 cut(s)	714, 992, 1213, 1617
BsmBI	CGTCTC	1 cut(s)	992
BsmFI	GGGAC	2 cut(s)	304, 941
BsnI	GGCC	4 cut(s)	447, 1499, 1651, 1694
Bso31I	GGTCTC	1 cut(s)	714
Bsp1286I	GDGCHC	1 cut(s)	1606
Bsp1407I	TGTACA	1 cut(s)	1091
Bsp143I	GATC	4 cut(s)	514, 799, 1947, 2044
BspACI	CCGC	3 cut(s)	381, 1712, 2035
BspANI	GGCC	4 cut(s)	447, 1499, 1651, 1694
BspCNI	CTCAG	1 cut(s)	1223
BspLI	GGNNCC	5 cut(s)	27, 446, 1044, 1693, 2046
BspMAI	CTGCAG	2 cut(s)	81, 1989
BspMI	ACCTGC	2 cut(s)	70, 1992
BspPI	GGATC	4 cut(s)	794, 1942, 2039, 2052
BspQI	GCTCTTC	1 cut(s)	1430
BspTI	CTTAAG	1 cut(s)	212
BspTNI	GGTCTC	1 cut(s)	714
BsrDI	GCAATG	2 cut(s)	1114, 2091
BsrGI	TGTACA	1 cut(s)	1091
BsrI	ACTGG	5 cut(s)	442, 684, 846, 1074, 1775
BssECI	CCNNGG	5 cut(s)	110, 356, 422, 923, 1871
BssMI	GATC	4 cut(s)	514, 799, 1947, 2044
BssNI	GRCGYC	1 cut(s)	385
Bst2UI	CCWGG	4 cut(s)	423, 1596, 1872, 1893
Bst4CI	ACNGT	8 cut(s)	295, 301, 820, 1096, 1184, 1899, 1907, 2012
Bst6I	CTCTTC	4 cut(s)	224, 907, 939, 1430
BstACI	GRCGYC	1 cut(s)	385
BstAFI	CTTAAG	1 cut(s)	212
BstAUI	TGTACA	1 cut(s)	1091
BstBAI	YACGTR	2 cut(s)	650, 1607
BstC8I	GCNNGC	4 cut(s)	383, 497, 573, 1566
BstDEI	CTNAG	2 cut(s)	782, 1210
BstF5I	GGATG	7 cut(s)	119, 247, 436, 446, 629, 1462, 1834
BstHHI	GCGC	1 cut(s)	1529
BstKTI	GATC	4 cut(s)	517, 802, 1950, 2047
BstMAI	GTCTC	4 cut(s)	714, 992, 1213, 1617
BstMBI	GATC	4 cut(s)	514, 799, 1947, 2044
BstMWI	GCNNNNNNNGC	5 cut(s)	644, 952, 974, 1001, 1466
BstNI	CCWGG	4 cut(s)	423, 1596, 1872, 1893
BstNSI	RCATGY	3 cut(s)	575, 1254, 1735
BstPAI	GACNNNNGTC	1 cut(s)	680
BstSCI	CCNGG	7 cut(s)	22, 109, 356, 421, 1594, 1870, 1891
BstSFI	CTRYAG	3 cut(s)	77, 1716, 1985
BstSLI	GKGCMC	1 cut(s)	1606
BstV1I	GCAGC	7 cut(s)	631, 727, 939, 952, 955, 1553, 1666
BstX2I	RGATCY	2 cut(s)	1947, 2044
BstYI	RGATCY	2 cut(s)	1947, 2044
BsuRI	GGCC	4 cut(s)	447, 1499, 1651, 1694
BtsCI	GGATG	7 cut(s)	119, 247, 436, 446, 629, 1462, 1834
BtsI	GCAGTG	2 cut(s)	713, 1102
BtsIMutI	CAGTG	6 cut(s)	297, 389, 677, 713, 1102, 1782
BveI	ACCTGC	2 cut(s)	70, 1992
Cac8I	GCNNGC	4 cut(s)	383, 497, 573, 1566
CaiI	CAGNNNCTG	2 cut(s)	968, 1535
CfoI	GCGC	1 cut(s)	1529
Cfr13I	GGNCC	7 cut(s)	26, 350, 445, 1358, 1498, 1692, 1759
CseI	GACGC	2 cut(s)	374, 1808
CviAII	CATG	8 cut(s)	317, 572, 992, 1039, 1199, 1251, 1580, 1732
DdeI	CTNAG	2 cut(s)	782, 1210
DpnI	GATC	4 cut(s)	516, 801, 1949, 2046
DpnII	GATC	4 cut(s)	514, 799, 1947, 2044
DraI	TTTAAA	1 cut(s)	852
DrdI	GACNNNNNNGTC	1 cut(s)	865
DseDI	GACNNNNNNGTC	1 cut(s)	865
Eam1104I	CTCTTC	4 cut(s)	224, 907, 939, 1430
EarI	CTCTTC	4 cut(s)	224, 907, 939, 1430
Eco31I	GGTCTC	1 cut(s)	714
Eco47I	GGWCC	4 cut(s)	26, 350, 1358, 1759
Eco57I	CTGAAG	3 cut(s)	413, 968, 1026
Eco72I	CACGTG	1 cut(s)	1607
EcoO109I	RGGNCCY	4 cut(s)	26, 445, 1358, 1759
EcoRI	GAATTC	2 cut(s)	46, 1238
EcoRII	CCWGG	4 cut(s)	421, 1594, 1870, 1891
Esp3I	CGTCTC	1 cut(s)	992
FaeI	CATG	8 cut(s)	320, 575, 995, 1042, 1202, 1254, 1583, 1735
FaqI	GGGAC	2 cut(s)	304, 941
FatI	CATG	8 cut(s)	316, 571, 991, 1038, 1198, 1250, 1579, 1731
FauI	CCCGC	2 cut(s)	374, 2028
Fnu4HI	GCNGC	8 cut(s)	645, 741, 953, 966, 969, 1542, 1680, 1713
FokI	GGATG	7 cut(s)	106, 254, 443, 453, 636, 1449, 1841
Fsp4HI	GCNGC	8 cut(s)	645, 741, 953, 966, 969, 1542, 1680, 1713
FspBI	CTAG	7 cut(s)	1013, 1023, 1133, 1191, 1227, 1736, 2099
GlaI	GCGC	1 cut(s)	1528
GluI	GCNGC	8 cut(s)	645, 741, 953, 966, 969, 1542, 1680, 1713
GsuI	CTGGAG	1 cut(s)	1362
HaeIII	GGCC	4 cut(s)	447, 1499, 1651, 1694
HapII	CCGG	3 cut(s)	23, 110, 358
HgaI	GACGC	2 cut(s)	374, 1808
HhaI	GCGC	1 cut(s)	1529
Hin1I	GRCGYC	1 cut(s)	385
Hin1II	CATG	8 cut(s)	320, 575, 995, 1042, 1202, 1254, 1583, 1735
Hin6I	GCGC	1 cut(s)	1527
HinP1I	GCGC	1 cut(s)	1527
HincII	GTYRAC	2 cut(s)	823, 858
HindII	GTYRAC	2 cut(s)	823, 858
HindIII	AAGCTT	1 cut(s)	59
HinfI	GANTC	9 cut(s)	104, 125, 398, 406, 611, 1610, 1811, 1832, 1876
HpaI	GTTAAC	1 cut(s)	823
HpaII	CCGG	3 cut(s)	23, 110, 358
HphI	GGTGA	6 cut(s)	99, 550, 809, 1640, 1792, 2084
Hpy166II	GTNNAC	6 cut(s)	74, 350, 389, 823, 858, 1604
Hpy188I	TCNGA	7 cut(s)	52, 66, 124, 405, 519, 1213, 1999
Hpy188III	TCNNGA	7 cut(s)	254, 1052, 1349, 1748, 1808, 1951, 2099
Hpy8I	GTNNAC	6 cut(s)	74, 350, 389, 823, 858, 1604
Hpy99I	CGWCG	2 cut(s)	820, 1188
HpyAV	CCTTC	5 cut(s)	388, 469, 524, 852, 1349
HpyCH4III	ACNGT	8 cut(s)	295, 301, 820, 1096, 1184, 1899, 1907, 2012
HpyCH4IV	ACGT	4 cut(s)	649, 1075, 1606, 1854
HpyF10VI	GCNNNNNNNGC	5 cut(s)	644, 952, 974, 1001, 1466
HpyF3I	CTNAG	2 cut(s)	782, 1210
HpySE526I	ACGT	4 cut(s)	649, 1075, 1606, 1854
Hsp92I	GRCGYC	1 cut(s)	385
Hsp92II	CATG	8 cut(s)	320, 575, 995, 1042, 1202, 1254, 1583, 1735
HspAI	GCGC	1 cut(s)	1527
KspAI	GTTAAC	1 cut(s)	823
Kzo9I	GATC	4 cut(s)	514, 799, 1947, 2044
LguI	GCTCTTC	1 cut(s)	1430
Lsp1109I	GCAGC	7 cut(s)	631, 727, 939, 952, 955, 1553, 1666
LweI	GCATC	3 cut(s)	14, 482, 614
MaeI	CTAG	7 cut(s)	1013, 1023, 1133, 1191, 1227, 1736, 2099
MaeII	ACGT	4 cut(s)	649, 1075, 1606, 1854
MaeIII	GTNAC	4 cut(s)	295, 630, 867, 1784
MalI	GATC	4 cut(s)	516, 801, 1949, 2046
MboI	GATC	4 cut(s)	514, 799, 1947, 2044
MfeI	CAATTG	1 cut(s)	1698
MflI	RGATCY	2 cut(s)	1947, 2044
MhlI	GDGCHC	1 cut(s)	1606
MlyI	GAGTC	2 cut(s)	1619, 1841
MmeI	TCCRAC	1 cut(s)	827
MroXI	GAANNNNTTC	2 cut(s)	1743, 2060
MslI	CAYNNNNRTG	3 cut(s)	1107, 1203, 1251
MspA1I	CMGCKG	1 cut(s)	965
MspCI	CTTAAG	1 cut(s)	212
MspI	CCGG	3 cut(s)	23, 110, 358
MspR9I	CCNGG	7 cut(s)	24, 111, 358, 423, 1596, 1872, 1893
MunI	CAATTG	1 cut(s)	1698
MvaI	CCWGG	4 cut(s)	423, 1596, 1872, 1893
MwoI	GCNNNNNNNGC	5 cut(s)	644, 952, 974, 1001, 1466
NciI	CCSGG	3 cut(s)	24, 111, 358
NdeII	GATC	4 cut(s)	514, 799, 1947, 2044
NlaIII	CATG	8 cut(s)	320, 575, 995, 1042, 1202, 1254, 1583, 1735
NlaIV	GGNNCC	5 cut(s)	27, 446, 1044, 1693, 2046
NmuCI	GTSAC	3 cut(s)	295, 630, 867
NspI	RCATGY	3 cut(s)	575, 1254, 1735
PacI	TTAATTAA	1 cut(s)	2028
PaeI	GCATGC	1 cut(s)	575
PciI	ACATGT	1 cut(s)	1250
PciSI	GCTCTTC	1 cut(s)	1430
PdmI	GAANNNNTTC	2 cut(s)	1743, 2060
PfeI	GAWTC	7 cut(s)	104, 125, 398, 406, 611, 1811, 1876
PkrI	GCNGC	8 cut(s)	646, 742, 954, 967, 970, 1543, 1681, 1714
PleI	GAGTC	2 cut(s)	1618, 1840
PmaCI	CACGTG	1 cut(s)	1607
PmlI	CACGTG	1 cut(s)	1607
PpsI	GAGTC	2 cut(s)	1618, 1840
Ppu21I	YACGTR	2 cut(s)	650, 1607
PpuMI	RGGWCCY	3 cut(s)	26, 1358, 1759
PscI	ACATGT	1 cut(s)	1250
PshAI	GACNNNNGTC	1 cut(s)	680
PsiI	TTATAA	1 cut(s)	1163
Psp5II	RGGWCCY	3 cut(s)	26, 1358, 1759
Psp6I	CCWGG	4 cut(s)	421, 1594, 1870, 1891
PspCI	CACGTG	1 cut(s)	1607
PspGI	CCWGG	4 cut(s)	421, 1594, 1870, 1891
PspN4I	GGNNCC	5 cut(s)	27, 446, 1044, 1693, 2046
PspPI	GGNCC	7 cut(s)	26, 350, 445, 1358, 1498, 1692, 1759
PspPPI	RGGWCCY	3 cut(s)	26, 1358, 1759
PstI	CTGCAG	2 cut(s)	81, 1989
PstNI	CAGNNNCTG	2 cut(s)	968, 1535
PsuI	RGATCY	2 cut(s)	1947, 2044
PvuII	CAGCTG	1 cut(s)	965
RseI	CAYNNNNRTG	3 cut(s)	1107, 1203, 1251
SapI	GCTCTTC	1 cut(s)	1430
SatI	GCNGC	8 cut(s)	645, 741, 953, 966, 969, 1542, 1680, 1713
Sau3AI	GATC	4 cut(s)	514, 799, 1947, 2044
Sau96I	GGNCC	7 cut(s)	26, 350, 445, 1358, 1498, 1692, 1759
ScaI	AGTACT	1 cut(s)	672
SchI	GAGTC	2 cut(s)	1619, 1841
ScrFI	CCNGG	7 cut(s)	24, 111, 358, 423, 1596, 1872, 1893
SduI	GDGCHC	1 cut(s)	1606
SfaNI	GCATC	3 cut(s)	14, 482, 614
SfcI	CTRYAG	3 cut(s)	77, 1716, 1985
SinI	GGWCC	4 cut(s)	26, 350, 1358, 1759
SmiMI	CAYNNNNRTG	3 cut(s)	1107, 1203, 1251
SmlI	CTYRAG	3 cut(s)	212, 566, 956
SmoI	CTYRAG	3 cut(s)	212, 566, 956
SphI	GCATGC	1 cut(s)	575
SsiI	CCGC	3 cut(s)	381, 1712, 2035
SspMI	CTAG	7 cut(s)	1013, 1023, 1133, 1191, 1227, 1736, 2099
StyD4I	CCNGG	7 cut(s)	22, 109, 356, 421, 1594, 1870, 1891
TaaI	ACNGT	8 cut(s)	295, 301, 820, 1096, 1184, 1899, 1907, 2012
TaiI	ACGT	4 cut(s)	652, 1078, 1609, 1857
TaqI	TCGA	7 cut(s)	156, 490, 549, 1051, 1835, 1926, 2064
TatI	WGTACW	3 cut(s)	73, 670, 1091
TauI	GCSGC	1 cut(s)	1715
TfiI	GAWTC	7 cut(s)	104, 125, 398, 406, 611, 1811, 1876
TscAI	CASTG	6 cut(s)	304, 396, 684, 720, 1109, 1782
TseFI	GTSAC	3 cut(s)	295, 630, 867
TseI	GCWGC	7 cut(s)	644, 740, 952, 965, 968, 1541, 1679
Tsp45I	GTSAC	3 cut(s)	295, 630, 867
TspDTI	ATGAA	8 cut(s)	305, 567, 768, 1068, 1071, 1272, 1368, 1781
TspGWI	ACGGA	2 cut(s)	139, 1282
TspRI	CASTG	6 cut(s)	304, 396, 684, 720, 1109, 1782
Vha464I	CTTAAG	1 cut(s)	212
VneI	GTGCAC	1 cut(s)	1602
VpaK11BI	GGWCC	4 cut(s)	26, 350, 1358, 1759
XapI	RAATTY	6 cut(s)	46, 542, 979, 1238, 1421, 1664
XbaI	TCTAGA	1 cut(s)	2098
XceI	RCATGY	3 cut(s)	575, 1254, 1735
XcmI	CCANNNNNNNNNTGG	2 cut(s)	467, 1473
XmnI	GAANNNNTTC	2 cut(s)	1743, 2060
XspI	CTAG	7 cut(s)	1013, 1023, 1133, 1191, 1227, 1736, 2099
ZrmI	AGTACT	1 cut(s)	672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.