Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004945

Physical Location & Seq

Genomic Coordinates Reverse (-)

46850103 .. 46857676

Gene Structure 7574 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025582

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGCATCAGTTATAGGTTTTGGGGGACCTCACTTTTTTGCATCAAGTTCTGAGAATAAGCCTTGGTTTCGAGTGCAAGTTAGGAGTTTAGGTAATCAGGATGCATCAGTTTTGCCTTCAGACTCAGTTGTAGTGAATGGAGCTTCTGTAGTTGGGGAGAGGGAGGGAAGTAGGCCTTTGGTTGATCAAGGGAAAGGGAGTTTGAGGCACAAGGTTAAGGAGAAACAGAGGCTAGAAGATGGTGTTCTCGAAAGTTTGGAACCTTTGTGGGATGATGGGTATGGGACTGTGACTGTAAAGGATTATTTCGATGCAACTGAGGAGATGATTAAGCCTGATGGTGGTCCTCCCCGATGGTTTAGCCCCCTTGCGCGCAACCCTCCATTGAAGGATTCTCCAGTTCTTTTGTTTTTGCCTGGGTTAGATGGTACTGGTTGCGGACTCATTTTGCATCATAAAGCTCTTCAGAAGGTCTTTGAAGTTCGATGCTTGCATATTCCAGTTAATGATCGAACACCATTTGAAGGGCTGGTGAAATTGGTGGAAGAAACTATTAGGGTTGAGCATGCTTCATCTCCCACCAAGCCAATTTATTTGGTAGGAGATTCTTTTGGAGGATGCCTATCACTTGCCGTTGCTGCACGTAATCCTACCATTGACCTAGTACTGATACTAGTCAACCCAGCAACATCCATCGAGAGGTCTCAGTTGCTACCTCTGTTCCCAATCTTGGAGGGTATGCCTGAAGAATTACATATCACAGTTCCCTATCTTCTCAGCTTTGTTATGGGTGATCCAATAAAAATGGCGACGGTTAATATAAAAAATAGGCTTCCTCCCGGTTTAAAACTTACGCAAATATCGCAGAACCTCACTGCTTTGCTACCATGTCTCTCTTTCTCTAACTTACTTTTAAACAGCTACTTACTGTCAAGTGTGTCAACATATGGTGAGGCACACACCTTATTCTCGACATATTCATTGAGACTGGTGCATTTTACTCTGAAACAGCTTAATGTTGAGAAGCTTAACTCTAGAGCATCACTTGACAAGGATAACATGGTTCCTAGTCGGGATGAAGCTGAACGACTAAGGAACTCATTACAGAACTGTTCAATTCATCACTTCCCAAACAATGGGCATACCCTATTACTGGAAGATGGGGTTAATTTGTTGACAGTTATAAAACGGACTCTCAAATATCGCCGTTCGAGGAAGCATGATAGTGTCTCAGATTTCCTTCCTCCTAGTATGACAGAATTCAACTATGCATTTGATGAAACAGTCGGATTGTTACGCTTTGCTGCTGGTTCTGCTCTTTTTTCAACATTGGAAGATGGAACGGTAGTGAGAGGTCTTGCAGGAGTTCCAATTGAAGGACCTGTCTTAATTGTTGGCTATCATAATTTAATGGGGCTAGAACTGTATTCTTTAGTTGAAGAATTTTTAAGAGAAAAGAAAATAATGGTTCGTGGTTTAGCGCATCCAGAACTGTTGTTGGGGAAGAGCTCTGCTGATTTTGACTTGCTCAAATTATTTGGTGCATTACCTGTTACGGCAAGAAATCTATTCAAAGCGCTTTCATCAAACTCACATGTTCTCTTGTATCCTGGTGGTGCACGTGAAGCTCTCCATTACAAGGGTGAAGAATACAAGTTATTCTGGCCTGATCAACCTGAATTTGTCAGAATGGCTGTACGTTTTGGGGCCACAATTGTACCATTTGCAGCTGTAGGAGAAGATGACTTACTCGAATTGGTTCTCGACTACAATGACATAAAGAAGATCCCTGTGGTGGGCGACTATTTCACCAGTGCTAATAATGATGCTATAAAATTGAGGGAGGATACACCTGGAGAAGTTGCCAACCAAGATCTCTTCTTTCCGGGAATTCTGCCCAAGTTACCAGGCCGCTATTACCATCTGTTTGGGAAACCGATAGAAACAAAAGGAAGGGAAGAGATCCTGAAAGACAAAGACAGTGCAAACAAATTGTATTTGGAGATACAATCTGAAATTGAACGAATTTTAGCATACTTAATCAAGAAGCGAGAGGAGGATCCTTACAGGAATCTTATTGACAGAACAGTATATCGGGCACTATATTCTCATTTAGATGAAATTCCAGCATTTGAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGAT Hydrolase_4

Protein Analysis

714

Amino Acids

79.54

Weight (kDa)

6.0

Isoelectric Point (pI)

44.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGAT	PF03982	512 - 587	2.6e-07	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54570
fragaria_vesca	FvH4_6g03990 FvH4_6g04000
malus_domestica	MD04G1216900.v1.1 MD12G1233500.v1.1
prunus_persica	Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1
pyrus_communis	pycom04g19300 pycom12g21610
rosa_chinensis	RchiOBHm_Chr3g0452581
rosa_laevigata	RLG00000014771 RLG00000025582
rosa_multiflora	Rmu_co8485569.1_g000001 Rmu_sc0004414.1_g000004 Rmu_sc0028651.1_g000001 Rmu_sc0032164.1_g000001
rosa_roxburghii	Rroxscaffold_6G00429620 Rroxscaffold_7G00209280 Rroxscaffold_7G00209300
rosa_rugosa	Rorug02G0641400 Rorug02G0641500 Rorug02G0641500
rosa_samantha	Rh3AG044000 Rh3BG046100 Rh3CG044800 Rh3DG045100
rosa_wichuraiana	Rw3G003410 Rw6G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1184
AccB7I	CCANNNNNTGG	1 cut(s)	1136
AccII	CGCG	1 cut(s)	373
AciI	CCGC	2 cut(s)	438, 1912
AclWI	GGATC	5 cut(s)	788, 1780, 1957, 2054, 2067
AcsI	RAATTY	6 cut(s)	1259, 1442, 1679, 1890, 2025, 2121
AcuI	CTGAAG	3 cut(s)	102, 449, 765
AcvI	CACGTG	1 cut(s)	1622
AfaI	GTAC	4 cut(s)	430, 666, 1698, 1719
AfeI	AGCGCT	1 cut(s)	1578
AflIII	ACRYGT	1 cut(s)	1594
AhlI	ACTAGT	1 cut(s)	673
AjnI	CCWGG	4 cut(s)	415, 1609, 1852, 1906
Alw21I	GWGCWC	2 cut(s)	1511, 1621
Alw26I	GTCTC	4 cut(s)	708, 896, 979, 1234
Alw44I	GTGCAC	1 cut(s)	1617
AlwI	GGATC	5 cut(s)	788, 1780, 1957, 2054, 2067
Aor51HI	AGCGCT	1 cut(s)	1578
AoxI	GGCC	4 cut(s)	173, 1664, 1707, 1909
ApaLI	GTGCAC	1 cut(s)	1617
ApeKI	GCWGC	3 cut(s)	638, 1304, 1727
ApoI	RAATTY	6 cut(s)	1259, 1442, 1679, 1890, 2025, 2121
Asp700I	GAANNNNTTC	1 cut(s)	1758
AspLEI	GCGC	4 cut(s)	373, 375, 1483, 1579
AspS9I	GGNCC	4 cut(s)	26, 344, 1379, 1707
AsuC2I	CCSGG	2 cut(s)	840, 1887
AsuHPI	GGTGA	5 cut(s)	544, 803, 962, 1655, 1801
AvaII	GGWCC	3 cut(s)	26, 344, 1379
BaeGI	GKGCMC	2 cut(s)	1621, 2101
BaeI	ACNNNNGTAYC	2 cut(s)	1701, 1734
BamHI	GGATCC	1 cut(s)	2059
BanII	GRGCYC	1 cut(s)	1511
BarI	GAAGNNNNNNTAC	2 cut(s)	1731, 1763
BbrPI	CACGTG	1 cut(s)	1622
Bbv12I	GWGCWC	2 cut(s)	1511, 1621
BbvI	GCAGC	3 cut(s)	625, 1291, 1739
BccI	CCATC	9 cut(s)	233, 269, 332, 348, 419, 701, 1154, 1331, 1929
BceAI	ACGGC	3 cut(s)	617, 1191, 1572
BciT130I	CCWGG	4 cut(s)	417, 1611, 1854, 1908
BciVI	GTATCC	2 cut(s)	1617, 1840
BclI	TGATCA	2 cut(s)	184, 1669
BcnI	CCSGG	2 cut(s)	840, 1887
BcoDI	GTCTC	4 cut(s)	708, 896, 979, 1234
BcuI	ACTAGT	1 cut(s)	673
BfaI	CTAG	7 cut(s)	233, 662, 674, 1035, 1068, 1248, 1418
BfmI	CTRYAG	2 cut(s)	147, 1731
BfoI	RGCGCY	1 cut(s)	1580
BfuI	GTATCC	2 cut(s)	1617, 1840
BglII	AGATCT	1 cut(s)	1873
BisI	GCNGC	4 cut(s)	639, 1305, 1728, 1912
BlsI	GCNGC	4 cut(s)	640, 1306, 1729, 1913
BmcAI	AGTACT	1 cut(s)	666
Bme1390I	CCNGG	6 cut(s)	417, 840, 1611, 1854, 1887, 1908
Bme18I	GGWCC	3 cut(s)	26, 344, 1379
BmgT120I	GGNCC	4 cut(s)	26, 344, 1379, 1707
BmiI	GGNNCC	5 cut(s)	27, 261, 1065, 1708, 2061
BmrFI	CCNGG	6 cut(s)	417, 840, 1611, 1854, 1887, 1908
BpmI	CTGGAG	2 cut(s)	381, 1875
BpuMI	CCSGG	2 cut(s)	840, 1887
BsaAI	YACGTR	2 cut(s)	644, 1622
BsaBI	GATNNNNATC	1 cut(s)	622
BsaI	GGTCTC	1 cut(s)	708
BsaJI	CCNNGG	2 cut(s)	62, 416
BsaXI	ACNNNNNCTCC	4 cut(s)	76, 106, 132, 162
Bse1I	ACTGG	6 cut(s)	398, 436, 500, 993, 1158, 1812
Bse8I	GATNNNNATC	1 cut(s)	622
BseBI	CCWGG	4 cut(s)	417, 1611, 1854, 1908
BseDI	CCNNGG	2 cut(s)	62, 416
BseGI	GGATG	6 cut(s)	106, 277, 623, 689, 1081, 1483
BseJI	GATNNNNATC	1 cut(s)	622
BseMII	CTCAG	6 cut(s)	42, 138, 309, 719, 790, 1245
BseNI	ACTGG	6 cut(s)	398, 436, 500, 993, 1158, 1812
BsePI	GCGCGC	1 cut(s)	371
BseRI	GAGGAG	2 cut(s)	335, 2069
BseSI	GKGCMC	2 cut(s)	1621, 2101
BseXI	GCAGC	3 cut(s)	625, 1291, 1739
BseYI	CCCAGC	1 cut(s)	682
BsgI	GTGCAG	1 cut(s)	624
Bsh1236I	CGCG	1 cut(s)	373
BshFI	GGCC	4 cut(s)	175, 1666, 1709, 1911
BsiHKAI	GWGCWC	2 cut(s)	1511, 1621
BsiSI	CCGG	2 cut(s)	840, 1886
BslFI	GGGAC	2 cut(s)	39, 298
BsmAI	GTCTC	4 cut(s)	708, 896, 979, 1234
BsmFI	GGGAC	2 cut(s)	39, 298
BsnI	GGCC	4 cut(s)	175, 1666, 1709, 1911
Bso31I	GGTCTC	1 cut(s)	708
Bsp1286I	GDGCHC	3 cut(s)	1511, 1621, 2101
Bsp143I	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
BspACI	CCGC	2 cut(s)	438, 1912
BspANI	GGCC	4 cut(s)	175, 1666, 1709, 1911
BspCNI	CTCAG	6 cut(s)	43, 137, 310, 718, 789, 1244
BspFNI	CGCG	1 cut(s)	373
BspLI	GGNNCC	5 cut(s)	27, 261, 1065, 1708, 2061
BspPI	GGATC	5 cut(s)	788, 1780, 1957, 2054, 2067
BspQI	GCTCTTC	2 cut(s)	468, 1499
BspTNI	GGTCTC	1 cut(s)	708
BsrI	ACTGG	6 cut(s)	398, 436, 500, 993, 1158, 1812
BssECI	CCNNGG	2 cut(s)	62, 416
BssHII	GCGCGC	1 cut(s)	371
BssMI	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
BssT1I	CCWWGG	1 cut(s)	62
Bst2UI	CCWGG	4 cut(s)	417, 1611, 1854, 1908
Bst6I	CTCTTC	4 cut(s)	468, 1499, 1883, 1953
BstBAI	YACGTR	2 cut(s)	644, 1622
BstC8I	GCNNGC	3 cut(s)	373, 491, 567
BstDEI	CTNAG	7 cut(s)	51, 124, 318, 705, 776, 1091, 1231
BstF5I	GGATG	6 cut(s)	106, 277, 623, 689, 1081, 1483
BstFNI	CGCG	1 cut(s)	373
BstH2I	RGCGCY	1 cut(s)	1580
BstHHI	GCGC	4 cut(s)	373, 375, 1483, 1579
BstKTI	GATC	8 cut(s)	187, 511, 796, 1672, 1788, 1876, 1965, 2062
BstMAI	GTCTC	4 cut(s)	708, 896, 979, 1234
BstMBI	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
BstMWI	GCNNNNNNNGC	4 cut(s)	638, 862, 1313, 1625
BstNI	CCWGG	4 cut(s)	417, 1611, 1854, 1908
BstNSI	RCATGY	2 cut(s)	569, 1598
BstSCI	CCNGG	6 cut(s)	415, 838, 1609, 1852, 1885, 1906
BstSFI	CTRYAG	2 cut(s)	147, 1731
BstSLI	GKGCMC	2 cut(s)	1621, 2101
BstUI	CGCG	1 cut(s)	373
BstV1I	GCAGC	3 cut(s)	625, 1291, 1739
BstX2I	RGATCY	4 cut(s)	1785, 1873, 1962, 2059
BstXI	CCANNNNNNTGG	1 cut(s)	1928
BstYI	RGATCY	4 cut(s)	1785, 1873, 1962, 2059
BsuI	GTATCC	2 cut(s)	1617, 1840
BsuRI	GGCC	4 cut(s)	175, 1666, 1709, 1911
BtsCI	GGATG	6 cut(s)	106, 277, 623, 689, 1081, 1483
BtsI	GCAGTG	1 cut(s)	873
BtsIMutI	CAGTG	3 cut(s)	873, 1819, 1987
Cac8I	GCNNGC	3 cut(s)	373, 491, 567
CfoI	GCGC	4 cut(s)	373, 375, 1483, 1579
Cfr13I	GGNCC	4 cut(s)	26, 344, 1379, 1707
Csp6I	GTAC	4 cut(s)	429, 665, 1697, 1718
CviAII	CATG	5 cut(s)	566, 888, 1060, 1220, 1595
CviQI	GTAC	4 cut(s)	429, 665, 1697, 1718
DdeI	CTNAG	7 cut(s)	51, 124, 318, 705, 776, 1091, 1231
DpnI	GATC	8 cut(s)	186, 510, 795, 1671, 1787, 1875, 1964, 2061
DpnII	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
DraI	TTTAAA	2 cut(s)	846, 915
Eam1104I	CTCTTC	4 cut(s)	468, 1499, 1883, 1953
EarI	CTCTTC	4 cut(s)	468, 1499, 1883, 1953
Ecl136II	GAGCTC	1 cut(s)	1509
Eco130I	CCWWGG	1 cut(s)	62
Eco147I	AGGCCT	1 cut(s)	175
Eco24I	GRGCYC	1 cut(s)	1511
Eco31I	GGTCTC	1 cut(s)	708
Eco47I	GGWCC	3 cut(s)	26, 344, 1379
Eco47III	AGCGCT	1 cut(s)	1578
Eco53kI	GAGCTC	1 cut(s)	1509
Eco57I	CTGAAG	3 cut(s)	102, 449, 765
Eco72I	CACGTG	1 cut(s)	1622
EcoICRI	GAGCTC	1 cut(s)	1509
EcoO109I	RGGNCCY	2 cut(s)	26, 1379
EcoRI	GAATTC	2 cut(s)	1259, 1890
EcoRII	CCWGG	4 cut(s)	415, 1609, 1852, 1906
EcoT14I	CCWWGG	1 cut(s)	62
EcoT22I	ATGCAT	2 cut(s)	106, 1273
EcoT38I	GRGCYC	1 cut(s)	1511
ErhI	CCWWGG	1 cut(s)	62
FaeI	CATG	5 cut(s)	569, 891, 1063, 1223, 1598
FalI	AAGNNNNNCTT	2 cut(s)	160, 192
FaqI	GGGAC	2 cut(s)	39, 298
FatI	CATG	5 cut(s)	565, 887, 1059, 1219, 1594
FauNDI	CATATG	1 cut(s)	946
FbaI	TGATCA	2 cut(s)	184, 1669
Fnu4HI	GCNGC	4 cut(s)	639, 1305, 1728, 1912
FokI	GGATG	6 cut(s)	113, 284, 630, 676, 1088, 1470
FriOI	GRGCYC	1 cut(s)	1511
Fsp4HI	GCNGC	4 cut(s)	639, 1305, 1728, 1912
FspBI	CTAG	7 cut(s)	233, 662, 674, 1035, 1068, 1248, 1418
GlaI	GCGC	4 cut(s)	372, 374, 1482, 1578
GluI	GCNGC	4 cut(s)	639, 1305, 1728, 1912
GsaI	CCCAGC	1 cut(s)	686
GsuI	CTGGAG	2 cut(s)	381, 1875
HaeII	RGCGCY	1 cut(s)	1580
HaeIII	GGCC	4 cut(s)	175, 1666, 1709, 1911
HapII	CCGG	2 cut(s)	840, 1886
HhaI	GCGC	4 cut(s)	373, 375, 1483, 1579
Hin1II	CATG	5 cut(s)	569, 891, 1063, 1223, 1598
Hin6I	GCGC	4 cut(s)	371, 373, 1481, 1577
HinP1I	GCGC	4 cut(s)	371, 373, 1481, 1577
HincII	GTYRAC	3 cut(s)	679, 942, 1176
HindII	GTYRAC	3 cut(s)	679, 942, 1176
HindIII	AAGCTT	1 cut(s)	1025
HinfI	GANTC	6 cut(s)	122, 392, 441, 605, 1192, 2071
HpaII	CCGG	2 cut(s)	840, 1886
HphI	GGTGA	5 cut(s)	544, 803, 962, 1655, 1801
Hpy166II	GTNNAC	4 cut(s)	679, 942, 1176, 1619
Hpy188I	TCNGA	8 cut(s)	52, 121, 468, 1005, 1234, 1289, 1688, 2014
Hpy8I	GTNNAC	4 cut(s)	679, 942, 1176, 1619
Hpy99I	CGWCG	1 cut(s)	814
HpyAV	CCTTC	7 cut(s)	126, 382, 463, 518, 1250, 1370, 1947
HpyCH4IV	ACGT	3 cut(s)	643, 1621, 1699
HpyF10VI	GCNNNNNNNGC	4 cut(s)	638, 862, 1313, 1625
HpyF3I	CTNAG	7 cut(s)	51, 124, 318, 705, 776, 1091, 1231
HpySE526I	ACGT	3 cut(s)	643, 1621, 1699
Hsp92II	CATG	5 cut(s)	569, 891, 1063, 1223, 1598
HspAI	GCGC	4 cut(s)	371, 373, 1481, 1577
Ksp22I	TGATCA	2 cut(s)	184, 1669
Kzo9I	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
LguI	GCTCTTC	2 cut(s)	468, 1499
LmnI	GCTCC	1 cut(s)	140
Lsp1109I	GCAGC	3 cut(s)	625, 1291, 1739
MaeI	CTAG	7 cut(s)	233, 662, 674, 1035, 1068, 1248, 1418
MaeII	ACGT	3 cut(s)	643, 1621, 1699
MaeIII	GTNAC	4 cut(s)	289, 1293, 1552, 1902
MalI	GATC	8 cut(s)	186, 510, 795, 1671, 1787, 1875, 1964, 2061
MboI	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
MfeI	CAATTG	2 cut(s)	1371, 1713
MflI	RGATCY	4 cut(s)	1785, 1873, 1962, 2059
MhlI	GDGCHC	3 cut(s)	1511, 1621, 2101
MlyI	GAGTC	3 cut(s)	116, 435, 1186
MmeI	TCCRAC	1 cut(s)	1267
Mph1103I	ATGCAT	2 cut(s)	106, 1273
MroXI	GAANNNNTTC	1 cut(s)	1758
MslI	CAYNNNNRTG	2 cut(s)	1224, 2115
MspA1I	CMGCKG	1 cut(s)	1730
MspI	CCGG	2 cut(s)	840, 1886
MspR9I	CCNGG	6 cut(s)	417, 840, 1611, 1854, 1887, 1908
MunI	CAATTG	2 cut(s)	1371, 1713
MvaI	CCWGG	4 cut(s)	417, 1611, 1854, 1908
MvnI	CGCG	1 cut(s)	373
MwoI	GCNNNNNNNGC	4 cut(s)	638, 862, 1313, 1625
NciI	CCSGG	2 cut(s)	840, 1887
NdeI	CATATG	1 cut(s)	946
NdeII	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
NlaIII	CATG	5 cut(s)	569, 891, 1063, 1223, 1598
NlaIV	GGNNCC	5 cut(s)	27, 261, 1065, 1708, 2061
NmuCI	GTSAC	1 cut(s)	289
NsiI	ATGCAT	2 cut(s)	106, 1273
NspI	RCATGY	2 cut(s)	569, 1598
PaeI	GCATGC	1 cut(s)	569
PauI	GCGCGC	1 cut(s)	371
PceI	AGGCCT	1 cut(s)	175
PciI	ACATGT	1 cut(s)	1594
PciSI	GCTCTTC	2 cut(s)	468, 1499
PdmI	GAANNNNTTC	1 cut(s)	1758
PfeI	GAWTC	3 cut(s)	392, 605, 2071
PflMI	CCANNNNNTGG	1 cut(s)	1136
PfoI	TCCNGGA	1 cut(s)	1885
PkrI	GCNGC	4 cut(s)	640, 1306, 1729, 1913
PleI	GAGTC	3 cut(s)	116, 435, 1186
PmaCI	CACGTG	1 cut(s)	1622
PmlI	CACGTG	1 cut(s)	1622
PpsI	GAGTC	3 cut(s)	116, 435, 1186
Ppu21I	YACGTR	2 cut(s)	644, 1622
PpuMI	RGGWCCY	2 cut(s)	26, 1379
PscI	ACATGT	1 cut(s)	1594
PsiI	TTATAA	1 cut(s)	1184
Psp124BI	GAGCTC	1 cut(s)	1511
Psp5II	RGGWCCY	2 cut(s)	26, 1379
Psp6I	CCWGG	4 cut(s)	415, 1609, 1852, 1906
PspCI	CACGTG	1 cut(s)	1622
PspFI	CCCAGC	1 cut(s)	682
PspGI	CCWGG	4 cut(s)	415, 1609, 1852, 1906
PspN4I	GGNNCC	5 cut(s)	27, 261, 1065, 1708, 2061
PspPI	GGNCC	4 cut(s)	26, 344, 1379, 1707
PspPPI	RGGWCCY	2 cut(s)	26, 1379
PsuI	RGATCY	4 cut(s)	1785, 1873, 1962, 2059
PteI	GCGCGC	1 cut(s)	371
PvuII	CAGCTG	1 cut(s)	1730
RsaI	GTAC	4 cut(s)	430, 666, 1698, 1719
RsaNI	GTAC	4 cut(s)	429, 665, 1697, 1718
RseI	CAYNNNNRTG	2 cut(s)	1224, 2115
SacI	GAGCTC	1 cut(s)	1511
SapI	GCTCTTC	2 cut(s)	468, 1499
SatI	GCNGC	4 cut(s)	639, 1305, 1728, 1912
Sau3AI	GATC	8 cut(s)	184, 508, 793, 1669, 1785, 1873, 1962, 2059
Sau96I	GGNCC	4 cut(s)	26, 344, 1379, 1707
ScaI	AGTACT	1 cut(s)	666
SchI	GAGTC	3 cut(s)	116, 435, 1186
ScrFI	CCNGG	6 cut(s)	417, 840, 1611, 1854, 1887, 1908
SduI	GDGCHC	3 cut(s)	1511, 1621, 2101
SfcI	CTRYAG	2 cut(s)	147, 1731
SinI	GGWCC	3 cut(s)	26, 344, 1379
SmiMI	CAYNNNNRTG	2 cut(s)	1224, 2115
SpeI	ACTAGT	1 cut(s)	673
SphI	GCATGC	1 cut(s)	569
SseBI	AGGCCT	1 cut(s)	175
SsiI	CCGC	2 cut(s)	438, 1912
SspMI	CTAG	7 cut(s)	233, 662, 674, 1035, 1068, 1248, 1418
SstI	GAGCTC	1 cut(s)	1511
StuI	AGGCCT	1 cut(s)	175
StyD4I	CCNGG	6 cut(s)	415, 838, 1609, 1852, 1885, 1906
StyI	CCWWGG	1 cut(s)	62
TaiI	ACGT	3 cut(s)	646, 1624, 1702
TatI	WGTACW	1 cut(s)	664
TauI	GCSGC	1 cut(s)	1914
TfiI	GAWTC	3 cut(s)	392, 605, 2071
TscAI	CASTG	3 cut(s)	880, 1819, 1987
TseFI	GTSAC	1 cut(s)	289
TseI	GCWGC	3 cut(s)	638, 1304, 1727
Tsp45I	GTSAC	1 cut(s)	289
TspDTI	ATGAA	7 cut(s)	561, 969, 1092, 1109, 1293, 1572, 2133
TspGWI	ACGGA	1 cut(s)	1204
TspRI	CASTG	3 cut(s)	880, 1819, 1987
Van91I	CCANNNNNTGG	1 cut(s)	1136
VneI	GTGCAC	1 cut(s)	1617
VpaK11BI	GGWCC	3 cut(s)	26, 344, 1379
XapI	RAATTY	6 cut(s)	1259, 1442, 1679, 1890, 2025, 2121
XbaI	TCTAGA	1 cut(s)	1034
XceI	RCATGY	2 cut(s)	569, 1598
XmnI	GAANNNNTTC	1 cut(s)	1758
XspI	CTAG	7 cut(s)	233, 662, 674, 1035, 1068, 1248, 1418
ZrmI	AGTACT	1 cut(s)	666
Zsp2I	ATGCAT	2 cut(s)	106, 1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.