Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004945

Physical Location & Seq

Genomic Coordinates Reverse (-)

30925175 .. 30930975

Gene Structure 5801 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1233500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGACATCAGTTATAGGCTTTCCGGGTCCGCATTTTTGTGTGCTGAATTTGGAGAATAAGCTTCGGTTTCGAGTGCAGGTTAGGAGTTTAGATAGTAGGGATTCACCAGTGTTGTCATCAGAATCATTTGTTTCTAATGGTGCTTCCGCAATCGAGGAGAGGGAGGATAGGAGAGGGAATGGGAGTTTGAGGTTTGAAGTTAAGGAGGAGGAGAAGAGGGTGGAGGATGGTGGTGGTCTTGAAAGGTTGAAACCTTTGTACGATGATGGGTATGGGACTGTCACTGTAAAAGATTATTTTCATGCAAACAAGGAGATGATTAAGCCTGACGGTGGACCTCCCCGGTGGTTTTCTCCCAATGCGTGCGGGCGCCCATTAAAGGATTCTCCGATTCTTTTGTTTTTGCCTGGGCTGGATGGTACTGGCTGGGGCCTCATTTTGCACCATAAAGCTCTTGGGAAGGCTTTTGAAGTTCGATGCCTGCATATTCCTGTTAATGATCGAACACCATTTGAAGGGCTGGTGAAATTTGTCGTAGAAACTATCAGGCTTGAGCATGCTTTATGTCCAAACAAGCCAATTTACTTGGTAGGAGATTCCTTCGGAGGATGCCTGTCACTTGCTGTTGCTGCACATAATCCTACCATTGATTTAGTACTGATACTAGTCAATCCAGCAACATCAGTTGAAAGGTCTCAGCTGCAACCTCTGTTCCCAATCTTGGAGGCTATGCCTGATGAATTACATTTTGCAGTTCCCTATCTACTTGGCTTTGTTATGGGTGATCCAACAAAAATGGCGATGGTTAACATTGAAAATAGGCTTCCTCCCAGTTTAAAGTTAACACAATTGTCTCGCAACCTGACTGATTTGCTACCATATCTCTCTTCTCTGGCTGATATTATACCGAGGGACACTCTTCTTTGGAAGCTGAAGCTGCTCAAATCAGCTGCTGCTTATGCAAATTCGCGTCTCCATGCTGTTAAAGCTGAAGTGCTAGTGTTGGCTAGTGGCAAAGATTATATGGTTCCAAGTCGAGATGAAGCAGAACGACTAGCACGTTCATTACAGAATTGTAAAGTCCGACACTTCAGTGACAATGGACATACTCTATTACTAGAAGATGGCGTTAATTTGATGTCAGTTATAAAAGGCACTTACAAATACCGTCGTTCAAGGAAGCATGATTATGTCTCAGATTTTCTTCCTCCTAGTATGTGGGAATTGAAAAATGCATCTGATAAACAATTCAGATTATTAAGCCTAGCTACTGCTTCCGTAATGCTCTCAACGTTGGAAGATGGGAAAATAGTGAGAGGTCTTGCTGGAGTTCCTAACGAAGGACCTGTCTTACTTGTTGGTTATCACAATTTATTGGGGTTGGAACTGAATTCTATAGTTGAAGAATTTTTAAGAGAGAAGAGCATAAGTGTTCGTGGATTAGCACATCCAGAACTGTTTTGGGGCAAGAACGGGACCTCGGCCCAATTTGACGTTCTCAAACTATTCGGTGCAGTACCTGTCGCAGCAAAAAACCTATTCAAATTGCTTAAAACAAAATCACATGTACTGCTTTATCCTGGTGGTGCACGTGAGGCTCTCCATAAGAAGGGTGAAGAATACAAGTTATTCTGGCCTGACCAACCGGAATTTGTGAGAATGGCTGCACGCTTTGGGGCCACAATTGTACCATTTGCGGCTGTAGGAGAAGATGACTTACTAGAATTGGTTCTTGACTACAATGACCTAATGAATATACCAGTGGTGAGTGACTATCTCAAAGACGCTAGTCGCGATACTATAAAACTAAGGGATGAGTCGAATGGGGAGGTTGCCAACGTTCAACTCTTTTTTCCAGGGGTTGTACCGAAGATACCAGGACGGTATTACTATTTGTTTGGAAAGCCCATAGAAACAAAGGGAAAGAAAGAGATCCTGCAAGACAAAGAAAACGCAAACGAATTGTACTTGCAGATACAATCTGACGTTGAAAACAGTTTAGCATACTTAATTAAGAAGCGGGAGGAGGATCCTTATAGGAATATTTTCGACAGAACAGCATATCGGGTAACGTATTCCTCTGTAGAGGAAGTTCCAACGTTTGAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGAT Hydrolase_4

Protein Analysis

704

Amino Acids

78.79

Weight (kDa)

6.63

Isoelectric Point (pI)

43.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	179 - 375	4e-06	alpha/beta hydrolase fold
Hydrolase_4	PF12146	189 - 374	2.5e-09	Serine aminopeptidase, S33
DAGAT	PF03982	498 - 578	1.1e-07	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54570
fragaria_vesca	FvH4_6g03990 FvH4_6g04000
malus_domestica	MD04G1216900.v1.1 MD12G1233500.v1.1
prunus_persica	Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1 Prupe.6G336800_v2.0.a1
pyrus_communis	pycom04g19300 pycom12g21610
rosa_chinensis	RchiOBHm_Chr3g0452581
rosa_laevigata	RLG00000014771 RLG00000025582
rosa_multiflora	Rmu_co8485569.1_g000001 Rmu_sc0004414.1_g000004 Rmu_sc0028651.1_g000001 Rmu_sc0032164.1_g000001
rosa_roxburghii	Rroxscaffold_6G00429620 Rroxscaffold_7G00209280 Rroxscaffold_7G00209300
rosa_rugosa	Rorug02G0641400 Rorug02G0641500 Rorug02G0641500
rosa_samantha	Rh3AG044000 Rh3BG046100 Rh3CG044800 Rh3DG045100
rosa_wichuraiana	Rw3G003410 Rw6G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1148
Acc36I	ACCTGC	1 cut(s)	67
AccB1I	GGYRCC	1 cut(s)	369
AccII	CGCG	2 cut(s)	970, 1794
AciI	CCGC	5 cut(s)	29, 147, 366, 1697, 2020
AclI	AACGTT	3 cut(s)	1292, 1839, 2099
AclWI	GGATC	4 cut(s)	779, 1927, 2024, 2037
AcsI	RAATTY	6 cut(s)	46, 527, 964, 1390, 1406, 1649
AcuI	CTGAAG	3 cut(s)	953, 1011, 1075
AcvI	CACGTG	1 cut(s)	1592
AcyI	GRCGYC	1 cut(s)	370
AfaI	GTAC	8 cut(s)	260, 421, 657, 1518, 1569, 1689, 1866, 1967
AfiI	CCNNNNNNNGG	6 cut(s)	332, 379, 515, 721, 1340, 1609
AflIII	ACRYGT	1 cut(s)	1564
AhlI	ACTAGT	1 cut(s)	664
AjnI	CCWGG	4 cut(s)	406, 1579, 1855, 1876
AjuI	GAANNNNNNNTTGG	2 cut(s)	1479, 1511
AleI	CACNNNNGTG	1 cut(s)	1092
AluBI	AGCT	8 cut(s)	61, 452, 700, 931, 937, 950, 989, 1268
AluI	AGCT	8 cut(s)	61, 452, 700, 931, 937, 950, 989, 1268
Alw21I	GWGCWC	1 cut(s)	1591
Alw26I	GTCTC	4 cut(s)	699, 858, 977, 1198
Alw44I	GTGCAC	1 cut(s)	1587
AlwI	GGATC	4 cut(s)	779, 1927, 2024, 2037
AlwNI	CAGNNNCTG	2 cut(s)	953, 1520
AoxI	GGCC	4 cut(s)	430, 1482, 1634, 1677
ApaLI	GTGCAC	1 cut(s)	1587
ApeKI	GCWGC	7 cut(s)	629, 700, 937, 950, 953, 1526, 1664
ApoI	RAATTY	6 cut(s)	46, 527, 964, 1390, 1406, 1649
ArsI	GACNNNNNNTTYG	2 cut(s)	1332, 1364
Asp700I	GAANNNNTTC	2 cut(s)	1728, 2045
AspLEI	GCGC	1 cut(s)	372
AspS9I	GGNCC	7 cut(s)	26, 335, 430, 1343, 1476, 1483, 1677
AsuC2I	CCSGG	2 cut(s)	24, 343
AsuHPI	GGTGA	5 cut(s)	96, 535, 794, 1625, 1777
AvaII	GGWCC	4 cut(s)	26, 335, 1343, 1476
BaeGI	GKGCMC	1 cut(s)	1591
BaeI	ACNNNNGTAYC	2 cut(s)	1671, 1704
BamHI	GGATCC	1 cut(s)	2029
BanI	GGYRCC	1 cut(s)	369
BarI	GAAGNNNNNNTAC	2 cut(s)	1701, 1733
BbrPI	CACGTG	1 cut(s)	1592
Bbv12I	GWGCWC	1 cut(s)	1591
BbvI	GCAGC	7 cut(s)	616, 687, 924, 937, 940, 1538, 1651
BccI	CCATC	6 cut(s)	221, 260, 410, 796, 1118, 1295
BciT130I	CCWGG	4 cut(s)	408, 1581, 1857, 1878
BcnI	CCSGG	2 cut(s)	24, 343
BcoDI	GTCTC	4 cut(s)	699, 858, 977, 1198
BcuI	ACTAGT	1 cut(s)	664
BfaI	CTAG	9 cut(s)	665, 998, 1008, 1055, 1118, 1212, 1265, 1721, 1788
BfmI	CTRYAG	3 cut(s)	1395, 1701, 2082
BfoI	RGCGCY	1 cut(s)	373
BfuAI	ACCTGC	1 cut(s)	67
BisI	GCNGC	8 cut(s)	630, 701, 938, 951, 954, 1527, 1665, 1698
BlsI	GCNGC	8 cut(s)	631, 702, 939, 952, 955, 1528, 1666, 1699
BmcAI	AGTACT	1 cut(s)	657
Bme1390I	CCNGG	6 cut(s)	24, 343, 408, 1581, 1857, 1878
Bme18I	GGWCC	4 cut(s)	26, 335, 1343, 1476
BmgT120I	GGNCC	7 cut(s)	26, 335, 430, 1343, 1476, 1483, 1677
BmiI	GGNNCC	7 cut(s)	27, 371, 431, 1029, 1477, 1678, 2031
BmrFI	CCNGG	6 cut(s)	24, 343, 408, 1581, 1857, 1878
BmrI	ACTGGG	1 cut(s)	825
BmsI	GCATC	3 cut(s)	467, 599, 1244
BmuI	ACTGGG	1 cut(s)	825
BoxI	GACNNNNGTC	1 cut(s)	1788
BplI	GAGNNNNNCTC	2 cut(s)	901, 933
BpmI	CTGGAG	1 cut(s)	1347
BpuEI	CTTGAG	1 cut(s)	572
BpuMI	CCSGG	2 cut(s)	24, 343
BsaAI	YACGTR	1 cut(s)	1592
BsaHI	GRCGYC	1 cut(s)	370
BsaI	GGTCTC	1 cut(s)	699
BsaJI	CCNNGG	5 cut(s)	341, 407, 908, 1479, 1856
BsaWI	WCCGGW	1 cut(s)	1645
Bsc4I	CCNNNNNNNGG	6 cut(s)	332, 379, 515, 721, 1340, 1609
Bse1I	ACTGG	4 cut(s)	107, 427, 831, 1760
BseBI	CCWGG	4 cut(s)	408, 1581, 1857, 1878
BseDI	CCNNGG	5 cut(s)	341, 407, 908, 1479, 1856
BseGI	GGATG	5 cut(s)	232, 421, 614, 1447, 1819
BseLI	CCNNNNNNNGG	6 cut(s)	332, 379, 515, 721, 1340, 1609
BseMII	CTCAG	2 cut(s)	710, 1209
BseNI	ACTGG	4 cut(s)	107, 427, 831, 1760
BseRI	GAGGAG	4 cut(s)	170, 221, 224, 2039
BseSI	GKGCMC	1 cut(s)	1591
BseXI	GCAGC	7 cut(s)	616, 687, 924, 937, 940, 1538, 1651
BseYI	CCCAGC	1 cut(s)	426
BsgI	GTGCAG	4 cut(s)	95, 615, 1533, 1650
Bsh1236I	CGCG	2 cut(s)	970, 1794
BshFI	GGCC	4 cut(s)	432, 1484, 1636, 1679
BshNI	GGYRCC	1 cut(s)	369
BsiHKAI	GWGCWC	1 cut(s)	1591
BsiSI	CCGG	3 cut(s)	23, 343, 1646
BslFI	GGGAC	3 cut(s)	289, 926, 1489
BslI	CCNNNNNNNGG	6 cut(s)	332, 379, 515, 721, 1340, 1609
BsmAI	GTCTC	4 cut(s)	699, 858, 977, 1198
BsmBI	CGTCTC	1 cut(s)	977
BsmFI	GGGAC	3 cut(s)	289, 926, 1489
BsnI	GGCC	4 cut(s)	432, 1484, 1636, 1679
Bso31I	GGTCTC	1 cut(s)	699
Bsp1286I	GDGCHC	1 cut(s)	1591
Bsp143I	GATC	4 cut(s)	499, 784, 1932, 2029
Bsp68I	TCGCGA	1 cut(s)	1794
BspACI	CCGC	5 cut(s)	29, 147, 366, 1697, 2020
BspANI	GGCC	4 cut(s)	432, 1484, 1636, 1679
BspCNI	CTCAG	2 cut(s)	709, 1208
BspFNI	CGCG	2 cut(s)	970, 1794
BspLI	GGNNCC	7 cut(s)	27, 371, 431, 1029, 1477, 1678, 2031
BspMI	ACCTGC	1 cut(s)	67
BspPI	GGATC	4 cut(s)	779, 1927, 2024, 2037
BspQI	GCTCTTC	1 cut(s)	1415
BspT107I	GGYRCC	1 cut(s)	369
BspTNI	GGTCTC	1 cut(s)	699
BsrI	ACTGG	4 cut(s)	107, 427, 831, 1760
BssECI	CCNNGG	5 cut(s)	341, 407, 908, 1479, 1856
BssMI	GATC	4 cut(s)	499, 784, 1932, 2029
BssNI	GRCGYC	1 cut(s)	370
Bst2UI	CCWGG	4 cut(s)	408, 1581, 1857, 1878
Bst4CI	ACNGT	7 cut(s)	280, 286, 332, 1169, 1458, 1884, 1997
Bst6I	CTCTTC	4 cut(s)	209, 892, 924, 1415
BstACI	GRCGYC	1 cut(s)	370
BstBAI	YACGTR	1 cut(s)	1592
BstC8I	GCNNGC	5 cut(s)	364, 368, 482, 558, 1669
BstDEI	CTNAG	3 cut(s)	696, 1195, 1808
BstENI	CCTNNNNNAGG	1 cut(s)	1338
BstF5I	GGATG	5 cut(s)	232, 421, 614, 1447, 1819
BstFNI	CGCG	2 cut(s)	970, 1794
BstH2I	RGCGCY	1 cut(s)	373
BstHHI	GCGC	1 cut(s)	372
BstKTI	GATC	4 cut(s)	502, 787, 1935, 2032
BstMAI	GTCTC	4 cut(s)	699, 858, 977, 1198
BstMBI	GATC	4 cut(s)	499, 784, 1932, 2029
BstMWI	GCNNNNNNNGC	5 cut(s)	629, 937, 959, 986, 1595
BstNI	CCWGG	4 cut(s)	408, 1581, 1857, 1878
BstNSI	RCATGY	2 cut(s)	560, 1568
BstPAI	GACNNNNGTC	1 cut(s)	1788
BstSCI	CCNGG	6 cut(s)	22, 341, 406, 1579, 1855, 1876
BstSFI	CTRYAG	3 cut(s)	1395, 1701, 2082
BstSLI	GKGCMC	1 cut(s)	1591
BstUI	CGCG	2 cut(s)	970, 1794
BstV1I	GCAGC	7 cut(s)	616, 687, 924, 937, 940, 1538, 1651
BstX2I	RGATCY	2 cut(s)	1932, 2029
BstYI	RGATCY	2 cut(s)	1932, 2029
BsuRI	GGCC	4 cut(s)	432, 1484, 1636, 1679
BtgZI	GCGATG	1 cut(s)	815
BtsCI	GGATG	5 cut(s)	232, 421, 614, 1447, 1819
BtsIMutI	CAGTG	4 cut(s)	114, 282, 1099, 1767
BtuMI	TCGCGA	1 cut(s)	1794
BveI	ACCTGC	1 cut(s)	67
Cac8I	GCNNGC	5 cut(s)	364, 368, 482, 558, 1669
CaiI	CAGNNNCTG	2 cut(s)	953, 1520
CfoI	GCGC	1 cut(s)	372
Cfr13I	GGNCC	7 cut(s)	26, 335, 430, 1343, 1476, 1483, 1677
CseI	GACGC	2 cut(s)	959, 1793
Csp6I	GTAC	8 cut(s)	259, 420, 656, 1517, 1568, 1688, 1865, 1966
CviAII	CATG	5 cut(s)	302, 557, 977, 1184, 1565
CviQI	GTAC	8 cut(s)	259, 420, 656, 1517, 1568, 1688, 1865, 1966
DdeI	CTNAG	3 cut(s)	696, 1195, 1808
DinI	GGCGCC	1 cut(s)	371
DpnI	GATC	4 cut(s)	501, 786, 1934, 2031
DpnII	GATC	4 cut(s)	499, 784, 1932, 2029
DraI	TTTAAA	1 cut(s)	837
Eam1104I	CTCTTC	4 cut(s)	209, 892, 924, 1415
EarI	CTCTTC	4 cut(s)	209, 892, 924, 1415
Eco31I	GGTCTC	1 cut(s)	699
Eco47I	GGWCC	4 cut(s)	26, 335, 1343, 1476
Eco57I	CTGAAG	3 cut(s)	953, 1011, 1075
Eco72I	CACGTG	1 cut(s)	1592
EcoNI	CCTNNNNNAGG	1 cut(s)	1338
EcoO109I	RGGNCCY	3 cut(s)	430, 1343, 1476
EcoRI	GAATTC	1 cut(s)	1390
EcoRII	CCWGG	4 cut(s)	406, 1579, 1855, 1876
EcoT22I	ATGCAT	1 cut(s)	1237
EgeI	GGCGCC	1 cut(s)	371
EheI	GGCGCC	1 cut(s)	371
Esp3I	CGTCTC	1 cut(s)	977
FaeI	CATG	5 cut(s)	305, 560, 980, 1187, 1568
FaqI	GGGAC	3 cut(s)	289, 926, 1489
FatI	CATG	5 cut(s)	301, 556, 976, 1183, 1564
FauI	CCCGC	2 cut(s)	359, 2013
Fnu4HI	GCNGC	8 cut(s)	630, 701, 938, 951, 954, 1527, 1665, 1698
FokI	GGATG	5 cut(s)	239, 428, 621, 1434, 1826
Fsp4HI	GCNGC	8 cut(s)	630, 701, 938, 951, 954, 1527, 1665, 1698
FspBI	CTAG	9 cut(s)	665, 998, 1008, 1055, 1118, 1212, 1265, 1721, 1788
GlaI	GCGC	1 cut(s)	371
GluI	GCNGC	8 cut(s)	630, 701, 938, 951, 954, 1527, 1665, 1698
GsaI	CCCAGC	1 cut(s)	430
GsuI	CTGGAG	1 cut(s)	1347
HaeII	RGCGCY	1 cut(s)	373
HaeIII	GGCC	4 cut(s)	432, 1484, 1636, 1679
HapII	CCGG	3 cut(s)	23, 343, 1646
HgaI	GACGC	2 cut(s)	959, 1793
HhaI	GCGC	1 cut(s)	372
Hin1I	GRCGYC	1 cut(s)	370
Hin1II	CATG	5 cut(s)	305, 560, 980, 1187, 1568
Hin6I	GCGC	1 cut(s)	370
HinP1I	GCGC	1 cut(s)	370
HincII	GTYRAC	2 cut(s)	808, 843
HindII	GTYRAC	2 cut(s)	808, 843
HindIII	AAGCTT	1 cut(s)	59
HinfI	GANTC	6 cut(s)	101, 122, 383, 391, 596, 1817
HpaI	GTTAAC	2 cut(s)	808, 843
HpaII	CCGG	3 cut(s)	23, 343, 1646
HphI	GGTGA	5 cut(s)	96, 535, 794, 1625, 1777
Hpy166II	GTNNAC	4 cut(s)	335, 808, 843, 1589
Hpy188I	TCNGA	8 cut(s)	121, 390, 605, 1085, 1198, 1240, 1253, 1984
Hpy188III	TCNNGA	5 cut(s)	239, 1037, 1451, 1733, 1793
Hpy8I	GTNNAC	4 cut(s)	335, 808, 843, 1589
Hpy99I	CGWCG	1 cut(s)	1173
HpyAV	CCTTC	5 cut(s)	454, 509, 610, 1334, 1603
HpyCH4III	ACNGT	7 cut(s)	280, 286, 332, 1169, 1458, 1884, 1997
HpyCH4IV	ACGT	8 cut(s)	1060, 1292, 1494, 1591, 1839, 1986, 2072, 2099
HpyF10VI	GCNNNNNNNGC	5 cut(s)	629, 937, 959, 986, 1595
HpyF3I	CTNAG	3 cut(s)	696, 1195, 1808
HpySE526I	ACGT	8 cut(s)	1060, 1292, 1494, 1591, 1839, 1986, 2072, 2099
Hsp92I	GRCGYC	1 cut(s)	370
Hsp92II	CATG	5 cut(s)	305, 560, 980, 1187, 1568
HspAI	GCGC	1 cut(s)	370
KasI	GGCGCC	1 cut(s)	369
KspAI	GTTAAC	2 cut(s)	808, 843
Kzo9I	GATC	4 cut(s)	499, 784, 1932, 2029
LguI	GCTCTTC	1 cut(s)	1415
Lsp1109I	GCAGC	7 cut(s)	616, 687, 924, 937, 940, 1538, 1651
LweI	GCATC	3 cut(s)	467, 599, 1244
MaeI	CTAG	9 cut(s)	665, 998, 1008, 1055, 1118, 1212, 1265, 1721, 1788
MaeII	ACGT	8 cut(s)	1060, 1292, 1494, 1591, 1839, 1986, 2072, 2099
MaeIII	GTNAC	5 cut(s)	280, 615, 1094, 1769, 2068
MalI	GATC	4 cut(s)	501, 786, 1934, 2031
MboI	GATC	4 cut(s)	499, 784, 1932, 2029
MfeI	CAATTG	2 cut(s)	848, 1683
MflI	RGATCY	2 cut(s)	1932, 2029
MhlI	GDGCHC	1 cut(s)	1591
Mly113I	GGCGCC	1 cut(s)	370
MlyI	GAGTC	1 cut(s)	1826
MmeI	TCCRAC	4 cut(s)	812, 1108, 1275, 1362
Mph1103I	ATGCAT	1 cut(s)	1237
MroXI	GAANNNNTTC	2 cut(s)	1728, 2045
MslI	CAYNNNNRTG	3 cut(s)	36, 1092, 1188
MspA1I	CMGCKG	2 cut(s)	700, 950
MspI	CCGG	3 cut(s)	23, 343, 1646
MspR9I	CCNGG	6 cut(s)	24, 343, 408, 1581, 1857, 1878
MunI	CAATTG	2 cut(s)	848, 1683
MvaI	CCWGG	4 cut(s)	408, 1581, 1857, 1878
MvnI	CGCG	2 cut(s)	970, 1794
MwoI	GCNNNNNNNGC	5 cut(s)	629, 937, 959, 986, 1595
NarI	GGCGCC	1 cut(s)	370
NciI	CCSGG	2 cut(s)	24, 343
NdeII	GATC	4 cut(s)	499, 784, 1932, 2029
NlaIII	CATG	5 cut(s)	305, 560, 980, 1187, 1568
NlaIV	GGNNCC	7 cut(s)	27, 371, 431, 1029, 1477, 1678, 2031
NmeAIII	GCCGAG	1 cut(s)	1460
NmuCI	GTSAC	4 cut(s)	280, 615, 1094, 1769
NruI	TCGCGA	1 cut(s)	1794
NsiI	ATGCAT	1 cut(s)	1237
NspI	RCATGY	2 cut(s)	560, 1568
OliI	CACNNNNGTG	1 cut(s)	1092
PacI	TTAATTAA	1 cut(s)	2013
PaeI	GCATGC	1 cut(s)	560
PciI	ACATGT	1 cut(s)	1564
PciSI	GCTCTTC	1 cut(s)	1415
PcsI	WCGNNNNNNNCGW	2 cut(s)	1057, 1791
PdmI	GAANNNNTTC	2 cut(s)	1728, 2045
PfeI	GAWTC	5 cut(s)	101, 122, 383, 391, 596
PkrI	GCNGC	8 cut(s)	631, 702, 939, 952, 955, 1528, 1666, 1699
PleI	GAGTC	1 cut(s)	1825
PluTI	GGCGCC	1 cut(s)	373
PmaCI	CACGTG	1 cut(s)	1592
PmlI	CACGTG	1 cut(s)	1592
PpsI	GAGTC	1 cut(s)	1825
Ppu21I	YACGTR	1 cut(s)	1592
PpuMI	RGGWCCY	2 cut(s)	1343, 1476
PscI	ACATGT	1 cut(s)	1564
PshAI	GACNNNNGTC	1 cut(s)	1788
PsiI	TTATAA	1 cut(s)	1148
Psp1406I	AACGTT	3 cut(s)	1292, 1839, 2099
Psp5II	RGGWCCY	2 cut(s)	1343, 1476
Psp6I	CCWGG	4 cut(s)	406, 1579, 1855, 1876
PspCI	CACGTG	1 cut(s)	1592
PspFI	CCCAGC	1 cut(s)	426
PspGI	CCWGG	4 cut(s)	406, 1579, 1855, 1876
PspN4I	GGNNCC	7 cut(s)	27, 371, 431, 1029, 1477, 1678, 2031
PspPI	GGNCC	7 cut(s)	26, 335, 430, 1343, 1476, 1483, 1677
PspPPI	RGGWCCY	2 cut(s)	1343, 1476
PsrI	GAACNNNNNNTAC	2 cut(s)	2076, 2108
PstNI	CAGNNNCTG	2 cut(s)	953, 1520
PsuI	RGATCY	2 cut(s)	1932, 2029
PvuII	CAGCTG	2 cut(s)	700, 950
RruI	TCGCGA	1 cut(s)	1794
RsaI	GTAC	8 cut(s)	260, 421, 657, 1518, 1569, 1689, 1866, 1967
RsaNI	GTAC	8 cut(s)	259, 420, 656, 1517, 1568, 1688, 1865, 1966
RseI	CAYNNNNRTG	3 cut(s)	36, 1092, 1188
SapI	GCTCTTC	1 cut(s)	1415
SatI	GCNGC	8 cut(s)	630, 701, 938, 951, 954, 1527, 1665, 1698
Sau3AI	GATC	4 cut(s)	499, 784, 1932, 2029
Sau96I	GGNCC	7 cut(s)	26, 335, 430, 1343, 1476, 1483, 1677
ScaI	AGTACT	1 cut(s)	657
SchI	GAGTC	1 cut(s)	1826
ScrFI	CCNGG	6 cut(s)	24, 343, 408, 1581, 1857, 1878
SduI	GDGCHC	1 cut(s)	1591
SfaNI	GCATC	3 cut(s)	467, 599, 1244
SfcI	CTRYAG	3 cut(s)	1395, 1701, 2082
SfoI	GGCGCC	1 cut(s)	371
SinI	GGWCC	4 cut(s)	26, 335, 1343, 1476
SmiMI	CAYNNNNRTG	3 cut(s)	36, 1092, 1188
SmlI	CTYRAG	1 cut(s)	551
SmoI	CTYRAG	1 cut(s)	551
SpeI	ACTAGT	1 cut(s)	664
SphI	GCATGC	1 cut(s)	560
SsiI	CCGC	5 cut(s)	29, 147, 366, 1697, 2020
SspDI	GGCGCC	1 cut(s)	369
SspI	AATATT	1 cut(s)	2044
SspMI	CTAG	9 cut(s)	665, 998, 1008, 1055, 1118, 1212, 1265, 1721, 1788
StyD4I	CCNGG	6 cut(s)	22, 341, 406, 1579, 1855, 1876
TaaI	ACNGT	7 cut(s)	280, 286, 332, 1169, 1458, 1884, 1997
TaiI	ACGT	8 cut(s)	1063, 1295, 1497, 1594, 1842, 1989, 2075, 2102
TaqI	TCGA	7 cut(s)	70, 153, 475, 502, 1036, 1820, 2049
TatI	WGTACW	3 cut(s)	655, 1567, 1965
TauI	GCSGC	1 cut(s)	1700
TfiI	GAWTC	5 cut(s)	101, 122, 383, 391, 596
TscAI	CASTG	4 cut(s)	114, 289, 1099, 1767
TseFI	GTSAC	4 cut(s)	280, 615, 1094, 1769
TseI	GCWGC	7 cut(s)	629, 700, 937, 950, 953, 1526, 1664
Tsp45I	GTSAC	4 cut(s)	280, 615, 1094, 1769
TspDTI	ATGAA	5 cut(s)	290, 753, 1053, 1056, 1766
TspGWI	ACGGA	1 cut(s)	1267
TspRI	CASTG	4 cut(s)	114, 289, 1099, 1767
VneI	GTGCAC	1 cut(s)	1587
VpaK11BI	GGWCC	4 cut(s)	26, 335, 1343, 1476
XagI	CCTNNNNNAGG	1 cut(s)	1338
XapI	RAATTY	6 cut(s)	46, 527, 964, 1390, 1406, 1649
XceI	RCATGY	2 cut(s)	560, 1568
XcmI	CCANNNNNNNNNTGG	2 cut(s)	452, 1458
XmnI	GAANNNNTTC	2 cut(s)	1728, 2045
XspI	CTAG	9 cut(s)	665, 998, 1008, 1055, 1118, 1212, 1265, 1721, 1788
ZrmI	AGTACT	1 cut(s)	657
Zsp2I	ATGCAT	1 cut(s)	1237

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.