FvH4_6g04510

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006533
Physical Location & Seq
Reverse (-)
2481422 .. 2482644
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04510.t1

Sequence Viewer

Length: 444 bp
ATGCTTGCAAGAAGAGGACCTTCCAGAGCATTGGCTGCAATAATATTGGATGAGAGTTACAAGAACTTGCTTCAGGAGACTGCTCACAGAGCTGGTTTAGAACTTCCTGTTTATACAACTGTTCGATTTGGACCAGGCCATGTTCCTGTTTTCTCATGCACAATTGAGCTAGGAGGAATGAATTTTATAGGGGATCCAGCTAGGACAAAGATACAAGCTCAGAAAAATGCTGCAGTAGCTGCTTGGACATCCCTGAAAAGACTGGCTCAACGTGATTCATCTTCTCCCTCAATGGAACAGGAACAAGTCAATATTTCTCCTATTCTCGGACATTTACGTCCATCTGAGCCTAAGAACCCTCGAAATGATCGCAGACATGGACAACATCGCACTCCCGTCTACAACAATTCTACCATGCCAATGCCGAGCCTATATCCGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

148

Amino Acids

16.33

Weight (kDa)

10.08

Isoelectric Point (pI)

71.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 20 - 84 1.6e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 336
AccI GTMKAC 1 cut(s) 399
AclWI GGATC 2 cut(s) 188, 201
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 326
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 5 cut(s) 92, 169, 200, 218, 239
AluI AGCT 5 cut(s) 92, 169, 200, 218, 239
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 2 cut(s) 188, 201
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 35, 230, 239
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 17, 131
AvaII GGWCC 2 cut(s) 17, 131
BamHI GGATCC 1 cut(s) 193
BbvI GCAGC 3 cut(s) 22, 217, 226
BccI CCATC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 2 cut(s) 170, 201
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 3 cut(s) 36, 231, 240
BlsI GCNGC 3 cut(s) 37, 232, 241
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 2 cut(s) 17, 131
BmgT120I GGNCC 2 cut(s) 17, 131
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 55, 248
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 2 cut(s) 233, 336
BseNI ACTGG 1 cut(s) 267
BseXI GCAGC 3 cut(s) 22, 217, 226
BshFI GGCC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 71
BsnI GGCC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 193, 367
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 232, 337
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 2 cut(s) 188, 201
BsrI ACTGG 1 cut(s) 267
BssMI GATC 2 cut(s) 193, 367
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 121
Bst6I CTCTTC 1 cut(s) 7
BstAPI GCANNNNNTGC 2 cut(s) 35, 239
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 219, 345, 351
BstF5I GGATG 2 cut(s) 55, 248
BstKTI GATC 2 cut(s) 196, 370
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 2 cut(s) 193, 367
BstMWI GCNNNNNNNGC 4 cut(s) 35, 89, 236, 239
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 3 cut(s) 22, 217, 226
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 31
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 2 cut(s) 55, 248
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 17, 131
CviAII CATG 4 cut(s) 140, 156, 377, 415
DdeI CTNAG 3 cut(s) 219, 345, 351
DpnI GATC 2 cut(s) 195, 369
DpnII GATC 2 cut(s) 193, 367
DrdI GACNNNNNNGTC 1 cut(s) 336
DseDI GACNNNNNNGTC 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 7
EarI CTCTTC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 17, 131
Eco57I CTGAAG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 133
FaeI CATG 4 cut(s) 143, 159, 380, 418
FaiI YATR 7 cut(s) 114, 141, 157, 188, 378, 416, 433
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 4 cut(s) 139, 155, 376, 414
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 36, 231, 240
FokI GGATG 2 cut(s) 62, 235
Fsp4HI GCNGC 3 cut(s) 36, 231, 240
FspBI CTAG 2 cut(s) 170, 201
GluI GCNGC 3 cut(s) 36, 231, 240
HaeIII GGCC 1 cut(s) 138
Hin1II CATG 4 cut(s) 143, 159, 380, 418
HinfI GANTC 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 4 cut(s) 222, 329, 346, 438
Hpy188III TCNNGA 2 cut(s) 24, 74
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 2 cut(s) 271, 337
HpyCH4V TGCA 4 cut(s) 8, 38, 159, 233
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 89, 236, 239
HpyF3I CTNAG 3 cut(s) 219, 345, 351
HpySE526I ACGT 2 cut(s) 271, 337
Hsp92II CATG 4 cut(s) 143, 159, 380, 418
Kzo9I GATC 2 cut(s) 193, 367
Lsp1109I GCAGC 3 cut(s) 22, 217, 226
MaeI CTAG 2 cut(s) 170, 201
MaeII ACGT 2 cut(s) 271, 337
MaeIII GTNAC 1 cut(s) 56
MalI GATC 2 cut(s) 195, 369
MboI GATC 2 cut(s) 193, 367
MboII GAAGA 2 cut(s) 24, 273
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 193
MluCI AATT 3 cut(s) 162, 181, 406
MnlI CCTC 4 cut(s) 8, 167, 298, 369
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 35, 89, 236, 239
NdeII GATC 2 cut(s) 193, 367
NlaIII CATG 4 cut(s) 143, 159, 380, 418
NlaIV GGNNCC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 3 cut(s) 37, 232, 241
PpuMI RGGWCCY 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 2 cut(s) 17, 131
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 419
SatI GCNGC 3 cut(s) 36, 231, 240
Sau3AI GATC 2 cut(s) 193, 367
Sau96I GGNCC 2 cut(s) 17, 131
ScrFI CCNGG 1 cut(s) 135
SetI ASST 8 cut(s) 22, 94, 171, 202, 220, 241, 274, 340
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 2 cut(s) 17, 131
SmiMI CAYNNNNRTG 1 cut(s) 419
Sse9I AATT 3 cut(s) 162, 181, 406
SspI AATATT 2 cut(s) 45, 313
SspMI CTAG 2 cut(s) 170, 201
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 2 cut(s) 274, 340
TaqI TCGA 2 cut(s) 124, 361
TasI AATT 3 cut(s) 162, 181, 406
TfiI GAWTC 1 cut(s) 275
TseI GCWGC 3 cut(s) 35, 230, 239
TspDTI ATGAA 2 cut(s) 194, 267
VpaK11BI GGWCC 2 cut(s) 17, 131
XapI RAATTY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 170, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.