Rh2BG681300

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006533
Physical Location & Seq
Forward (+)
89070657 .. 89075056
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG681300.1

Sequence Viewer

Length: 1455 bp
ATGTATAAGAACCAGCTGCAGGAGCTTGCACAGAGAAGCTGTTTCAACCTGCCTTCATATTCTTGCATCAGAGAAGGACCAAATCATGCACCAAGATTCAAAGCTTCTGTTAACTTCAACGGTGAGATCTTCGACAGCCCAAACTACTGCACCACACTGAGACAAGCAGAGCATTCTGCTGCTGAAGTAGCCCTCAATGTTCTTTCCACTAGAGGCCCTTCAAGGTCTTTAACTGCAAGAATTCTTGATGAAACTGGAATATACAAAAACCTGCTTCAAGAAACTGCTCGCAGAGCTGGGCTGAAGCTGCCTGTATACACTAATGTGAGATCAGGGCCAGGGCATGTCCCAACTTTCACTTGTAGTGTAGAGCTTGCTGGCATGAATTTTAATGGGGAGGCAGCAAAGACCAAGAAGCAAGCTGAAAAGAATGCAGCAATTGCTGCCTGGTCTGCCTTGAAAAGAATCTCACTGGTGGCAAACAAAGAAGGAGAGAGTAGTGGTGATCAAGAACAGCAGGAGCGAGCAGTTGTGACAAGGCTGCTCTCAAACAAAGAAGACCGTAGCAGACAGTTGAGGAGGAGACCAGATCCTCAAACTCAAGCAAGGAGAAGAACTGTGAGGGGTGCTCATAATCATAGTCATAGTTATAGGGACAACAATGCCTCCACCTCCACAAACTCTCTAGTACAGAACCTACAGCATTGGAGGCTCATAGATGATCTGTTAACGGATTTGGTTCCTCGAGTTTCAAGTAATTCACAGAAACAAACCAGCTTTGCGTCTCTTCTCCCTCCACCGCCACCGCGAAAGGAGTCCAAGATTTTGCCACCAACAGCTACACTCAACAAATCAAACAATTCCTCCTACTACAATTCCAGTTATAATAGGCCTGTTCCTGTCCAAATGAGGGGTAGTGGTAGATCACAGGTTGTTCCACCTCCATTGGAAGACCACCAAAAGGATGAGGAAGAATGGCGTGGTACAAAGTCAGATAGCTTGATCAGTAAGATGCCCAATACTGAAAAGGAAGGTCATAGTCCTAGCCCTAGTAATTATTTTTCAAATCCAAGCAGCCCTGTATTTGGTTCCAGTGTTGCACACATCAGGCAATTTCCTATGTCCCCAAATCCAGTTAAGGCACCAACTACTAGTGCAAGTGCAAGTTCAAGTTCAAGTTCCCTCCTGGGCAGAAGAAGGATGTACACTCAAGGGTTCAATCCTCACGGTCATAGGGTTGCCCCAGCAGTTCATATCAGATCGGTGATTCCTGTTTGTGCAGCACCATCTGTAGCAGTGAGACCCTTAGTAGTAACTCCATCATACCCACCACCATTACCGATATTACCAAGAATGAAAGAAACTTGTAGTACCAGTCCCAGTCCTGGTACTAGTGCTAGTACTAGTTCACAACCAGCAGCAGTAGCAGTCCTGAAAGAGAGTGATTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

484

Amino Acids

52.99

Weight (kDa)

9.96

Isoelectric Point (pI)

69.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 2 - 68 6.1e-15 Double-stranded RNA binding motif
dsrm PF00035 88 - 152 1.8e-15 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 885
Acc36I ACCTGC 2 cut(s) 57, 279
AccB1I GGYRCC 1 cut(s) 1141
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 808
AciI CCGC 2 cut(s) 800, 806
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 2 cut(s) 240, 385
AcuI CTGAAG 2 cut(s) 204, 323
AfaI GTAC 6 cut(s) 690, 985, 1205, 1372, 1390, 1402
AfiI CCNNNNNNNGG 5 cut(s) 19, 910, 961, 1085, 1385
AhlI ACTAGT 3 cut(s) 1151, 1391, 1403
AjnI CCWGG 4 cut(s) 337, 446, 1185, 1384
AleI CACNNNNGTG 1 cut(s) 323
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 4 cut(s) 154, 577, 789, 1294
AlwI GGATC 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 744
AoxI GGCC 3 cut(s) 214, 335, 890
ApoI RAATTY 2 cut(s) 240, 385
AspS9I GGNCC 3 cut(s) 77, 215, 335
AsuHPI GGTGA 3 cut(s) 134, 515, 1276
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 1141
BbsI GAAGAC 2 cut(s) 564, 957
Bbv12I GWGCWC 1 cut(s) 631
BccI CCATC 2 cut(s) 1294, 1327
BciT130I CCWGG 4 cut(s) 339, 448, 1187, 1386
BclI TGATCA 2 cut(s) 505, 1002
BcoDI GTCTC 4 cut(s) 154, 577, 789, 1294
BcuI ACTAGT 3 cut(s) 1151, 1391, 1403
BfaI CTAG 8 cut(s) 210, 686, 1044, 1050, 1152, 1392, 1398, 1404
BfmI CTRYAG 3 cut(s) 17, 698, 1290
BfuAI ACCTGC 2 cut(s) 57, 279
BglII AGATCT 1 cut(s) 126
BmcAI AGTACT 1 cut(s) 1402
Bme1390I CCNGG 4 cut(s) 339, 448, 1187, 1386
Bme18I GGWCC 1 cut(s) 77
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 3 cut(s) 77, 215, 335
BmiI GGNNCC 3 cut(s) 741, 1090, 1143
BmrFI CCNGG 4 cut(s) 339, 448, 1187, 1386
BmrI ACTGGG 1 cut(s) 1374
BmsI GCATC 2 cut(s) 75, 1002
BmuI ACTGGG 1 cut(s) 1374
BpiI GAAGAC 2 cut(s) 564, 957
BplI GAGNNNNNCTC 2 cut(s) 613, 645
BpuEI CTTGAG 2 cut(s) 585, 1194
BsaI GGTCTC 2 cut(s) 577, 1294
BsaJI CCNNGG 2 cut(s) 338, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 650, 680, 848, 878
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 910, 961, 1085, 1385
Bse1I ACTGG 7 cut(s) 259, 477, 879, 1092, 1133, 1374, 1380
BseBI CCWGG 4 cut(s) 339, 448, 1187, 1386
BseDI CCNNGG 2 cut(s) 338, 1186
BseGI GGATG 2 cut(s) 970, 1206
BseLI CCNNNNNNNGG 5 cut(s) 19, 910, 961, 1085, 1385
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 7 cut(s) 259, 477, 879, 1092, 1133, 1374, 1380
BseRI GAGGAG 2 cut(s) 592, 595
BseYI CCCAGC 2 cut(s) 296, 1243
BsgI GTGCAG 2 cut(s) 133, 1299
Bsh1236I CGCG 1 cut(s) 808
BshFI GGCC 3 cut(s) 216, 337, 892
BshNI GGYRCC 1 cut(s) 1141
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 744
BslFI GGGAC 4 cut(s) 332, 668, 1108, 1362
BslI CCNNNNNNNGG 5 cut(s) 19, 910, 961, 1085, 1385
BsmAI GTCTC 4 cut(s) 154, 577, 789, 1294
BsmBI CGTCTC 1 cut(s) 789
BsmFI GGGAC 4 cut(s) 332, 668, 1108, 1362
BsmI GAATGC 2 cut(s) 172, 436
BsnI GGCC 3 cut(s) 216, 337, 892
Bso31I GGTCTC 2 cut(s) 577, 1294
BsoBI CYCGRG 1 cut(s) 744
Bsp1286I GDGCHC 1 cut(s) 631
Bsp1407I TGTACA 1 cut(s) 1203
Bsp143I GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
BspACI CCGC 2 cut(s) 800, 806
BspANI GGCC 3 cut(s) 216, 337, 892
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 808
BspLI GGNNCC 3 cut(s) 741, 1090, 1143
BspMAI CTGCAG 1 cut(s) 21
BspMI ACCTGC 2 cut(s) 57, 279
BspPI GGATC 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 1141
BspTNI GGTCTC 2 cut(s) 577, 1294
BsrGI TGTACA 1 cut(s) 1203
BsrI ACTGG 7 cut(s) 259, 477, 879, 1092, 1133, 1374, 1380
BssECI CCNNGG 2 cut(s) 338, 1186
BssMI GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
BssNAI GTATAC 1 cut(s) 316
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 4 cut(s) 339, 448, 1187, 1386
Bst4CI ACNGT 5 cut(s) 122, 563, 573, 619, 1229
Bst6I CTCTTC 1 cut(s) 792
BstAPI GCANNNNNTGC 2 cut(s) 440, 443
BstAUI TGTACA 1 cut(s) 1203
BstC8I GCNNGC 6 cut(s) 27, 289, 375, 379, 420, 525
BstDEI CTNAG 2 cut(s) 158, 1306
BstF5I GGATG 2 cut(s) 970, 1206
BstFNI CGCG 1 cut(s) 808
BstKTI GATC 8 cut(s) 129, 332, 508, 592, 724, 926, 1005, 1262
BstMAI GTCTC 4 cut(s) 154, 577, 789, 1294
BstMBI GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
BstMWI GCNNNNNNNGC 9 cut(s) 22, 188, 293, 307, 440, 443, 452, 709, 1424
BstNI CCWGG 4 cut(s) 339, 448, 1187, 1386
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 4 cut(s) 337, 446, 1185, 1384
BstSFI CTRYAG 3 cut(s) 17, 698, 1290
BstUI CGCG 1 cut(s) 808
BstV2I GAAGAC 2 cut(s) 564, 957
BstX2I RGATCY 2 cut(s) 126, 589
BstYI RGATCY 2 cut(s) 126, 589
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 216, 337, 892
BtsCI GGATG 2 cut(s) 970, 1206
BtsI GCAGTG 1 cut(s) 1302
BtsIMutI CAGTG 4 cut(s) 155, 470, 1099, 1302
BveI ACCTGC 2 cut(s) 57, 279
Cac8I GCNNGC 6 cut(s) 27, 289, 375, 379, 420, 525
Cfr13I GGNCC 3 cut(s) 77, 215, 335
CseI GACGC 1 cut(s) 771
Csp6I GTAC 6 cut(s) 689, 984, 1204, 1371, 1389, 1401
CspCI CAANNNNNGTGG 2 cut(s) 927, 962
CviAII CATG 4 cut(s) 86, 344, 382, 1452
CviQI GTAC 6 cut(s) 689, 984, 1204, 1371, 1389, 1401
DdeI CTNAG 2 cut(s) 158, 1306
DpnI GATC 8 cut(s) 128, 331, 507, 591, 723, 925, 1004, 1261
DpnII GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco147I AGGCCT 1 cut(s) 892
Eco31I GGTCTC 2 cut(s) 577, 1294
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 204, 323
Eco88I CYCGRG 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 240
EcoRII CCWGG 4 cut(s) 337, 446, 1185, 1384
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 4 cut(s) 89, 347, 385, 1455
FaqI GGGAC 4 cut(s) 332, 668, 1108, 1362
FatI CATG 4 cut(s) 85, 343, 381, 1451
FbaI TGATCA 2 cut(s) 505, 1002
FblI GTMKAC 1 cut(s) 315
FokI GGATG 2 cut(s) 977, 1213
FspBI CTAG 8 cut(s) 210, 686, 1044, 1050, 1152, 1392, 1398, 1404
GsaI CCCAGC 2 cut(s) 300, 1247
HaeIII GGCC 3 cut(s) 216, 337, 892
HgaI GACGC 1 cut(s) 771
Hin1II CATG 4 cut(s) 89, 347, 385, 1455
HincII GTYRAC 2 cut(s) 112, 729
HindII GTYRAC 2 cut(s) 112, 729
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 5 cut(s) 96, 465, 815, 1267, 1445
HpaI GTTAAC 2 cut(s) 112, 729
HphI GGTGA 3 cut(s) 134, 515, 1276
Hpy166II GTNNAC 5 cut(s) 112, 316, 729, 1206, 1409
Hpy188I TCNGA 3 cut(s) 71, 994, 1259
Hpy188III TCNNGA 4 cut(s) 245, 278, 509, 1432
Hpy8I GTNNAC 5 cut(s) 112, 316, 729, 1206, 1409
HpyAV CCTTC 6 cut(s) 63, 68, 228, 482, 1025, 1191
HpyCH4III ACNGT 5 cut(s) 122, 563, 573, 619, 1229
HpyF10VI GCNNNNNNNGC 9 cut(s) 22, 188, 293, 307, 440, 443, 452, 709, 1424
HpyF3I CTNAG 2 cut(s) 158, 1306
Hsp92II CATG 4 cut(s) 89, 347, 385, 1455
Ksp22I TGATCA 2 cut(s) 505, 1002
KspAI GTTAAC 2 cut(s) 112, 729
Kzo9I GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
LmnI GCTCC 2 cut(s) 22, 520
LweI GCATC 2 cut(s) 75, 1002
MaeI CTAG 8 cut(s) 210, 686, 1044, 1050, 1152, 1392, 1398, 1404
MaeIII GTNAC 2 cut(s) 532, 1312
MalI GATC 8 cut(s) 128, 331, 507, 591, 723, 925, 1004, 1261
MboI GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
MboII GAAGA 7 cut(s) 121, 569, 624, 779, 962, 983, 1206
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 2 cut(s) 126, 589
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 8 cut(s) 240, 385, 438, 757, 859, 874, 1054, 1112
MlyI GAGTC 1 cut(s) 824
MseI TTAA 5 cut(s) 111, 230, 390, 728, 1137
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 16
MspR9I CCNGG 4 cut(s) 339, 448, 1187, 1386
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 2 cut(s) 172, 436
MvaI CCWGG 4 cut(s) 339, 448, 1187, 1386
MvnI CGCG 1 cut(s) 808
MwoI GCNNNNNNNGC 9 cut(s) 22, 188, 293, 307, 440, 443, 452, 709, 1424
NdeII GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
NlaIII CATG 4 cut(s) 89, 347, 385, 1455
NlaIV GGNNCC 3 cut(s) 741, 1090, 1143
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 1 cut(s) 347
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 744
PceI AGGCCT 1 cut(s) 892
PctI GAATGC 2 cut(s) 172, 436
PfeI GAWTC 4 cut(s) 96, 465, 1267, 1445
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PsiI TTATAA 1 cut(s) 885
Psp6I CCWGG 4 cut(s) 337, 446, 1185, 1384
PspFI CCCAGC 2 cut(s) 296, 1243
PspGI CCWGG 4 cut(s) 337, 446, 1185, 1384
PspN4I GGNNCC 3 cut(s) 741, 1090, 1143
PspPI GGNCC 3 cut(s) 77, 215, 335
PspXI VCTCGAGB 1 cut(s) 744
PstI CTGCAG 1 cut(s) 21
PsuI RGATCY 2 cut(s) 126, 589
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 6 cut(s) 690, 985, 1205, 1372, 1390, 1402
RsaNI GTAC 6 cut(s) 689, 984, 1204, 1371, 1389, 1401
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 5 cut(s) 111, 230, 390, 728, 1137
Sau3AI GATC 8 cut(s) 126, 329, 505, 589, 721, 923, 1002, 1259
Sau96I GGNCC 3 cut(s) 77, 215, 335
ScaI AGTACT 1 cut(s) 1402
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 4 cut(s) 339, 448, 1187, 1386
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 2 cut(s) 75, 1002
SfcI CTRYAG 3 cut(s) 17, 698, 1290
Sfr274I CTCGAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 600, 744, 1209
SmoI CTYRAG 3 cut(s) 600, 744, 1209
SpeI ACTAGT 3 cut(s) 1151, 1391, 1403
Sse9I AATT 8 cut(s) 240, 385, 438, 757, 859, 874, 1054, 1112
SseBI AGGCCT 1 cut(s) 892
SsiI CCGC 2 cut(s) 800, 806
SspMI CTAG 8 cut(s) 210, 686, 1044, 1050, 1152, 1392, 1398, 1404
StuI AGGCCT 1 cut(s) 892
StyD4I CCNGG 4 cut(s) 337, 446, 1185, 1384
TaaI ACNGT 5 cut(s) 122, 563, 573, 619, 1229
TaqI TCGA 2 cut(s) 132, 745
TasI AATT 8 cut(s) 240, 385, 438, 757, 859, 874, 1054, 1112
TatI WGTACW 3 cut(s) 688, 1203, 1400
TfiI GAWTC 4 cut(s) 96, 465, 1267, 1445
Tru1I TTAA 5 cut(s) 111, 230, 390, 728, 1137
Tru9I TTAA 5 cut(s) 111, 230, 390, 728, 1137
TscAI CASTG 4 cut(s) 162, 477, 1099, 1302
TseFI GTSAC 1 cut(s) 532
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 5 cut(s) 45, 264, 398, 1241, 1370
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 4 cut(s) 162, 477, 1099, 1302
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 2 cut(s) 240, 385
XceI RCATGY 1 cut(s) 347
XhoI CTCGAG 1 cut(s) 744
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 8 cut(s) 210, 686, 1044, 1050, 1152, 1392, 1398, 1404
ZrmI AGTACT 1 cut(s) 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.