FvH4_6g53980

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006533
Physical Location & Seq
Forward (+)
39480915 .. 39484710
3796 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53980.t1

Sequence Viewer

Length: 1473 bp
ATGTATAAGAACCAGCTGCAGGAGCTTGCACAGAGAAGCTGTTTCAACCTGCCTTCATATTCTTGCATCAGAGAAGGACCAGATCATGCACCAAGATTCAAAGCTTCTGTTAACTTCAACGGTGAGATCTTCGAGAGCCCAAACTACTGCACCACACTGAGGCAAGCAGAGCATTCTGCTGCTGAAGTAGCCCTCAATGTTCTTTCCACTAGAGGCCCTTCAAGGTCCTTAACTGCAAGAGTTCTTGATGAAACTGGAATTTATAAAAACCTGCTTCAAGAAACTGCTCACAGAGCTGGGCTGAAGCTGCCTGTTTACACTACTGTGAGATCAGGGCCTGGGCATGTCCCAACTTTCACTTGTAGTGTAGAGCTTGCTGGCATGAATTTTAATGGAGAGGCAGCAAAAACCAAGAAGCAAGCTGAGAAGAATGCTGCAATTGCTGCCTGGTCTGCCTTGAAAAGAAATTTGGGGTCAGTTTCACTAATGACAAACAAAGAAGGAGAAAGTGGTGGTGATCAAGAACAGCAGGAGCAGGCAGTTGTGACTAGGCTGCTCTCAAATTTTAGACTCTCAAGCAAAGAAGAATATAGCAGACAGTTGAGGAGGAAACCAGATCATCAACAAACTCAAGCAAGGAGACGAACTGTGAGGGGTGCTAATAATCATAGTCATATTCATAGGGACAACAATGCCTCCACTTCCAACTCTCTAGTACAGAATCTACAGCACTGGAGGCTCATAGATGAAATGCTAACAGATTTGGTTCCTCGAGTTTCTAGTACTTCACAGAAACAAAGCAGTTTTGCGTCTCTTCTTCCTCCACCGCCACCACGAATGGAGTCCAAGATTTTGCCACCAACAGCTACACTCAACAGCAAATCAGACAATTCCTCCTACTACAATTCCAGTTATAATAGGCCTATTCCTGTCCAAATGAGAGGTAGTAGTAGTAGGTCGCAGGTTGTTCCACCTCCATTGGAAGAGCACCAAAAGGATGAGGAAGAATGGCTTGGTGTGAAGTCAGATATCTTGATCGGTAAAAAGACCACTAGTGAAAAGGAAAGTCATAGTCCTAGCCATAGTAACTACTTTTCGAATCCAAGCAGACCTGCATTTGGTTCCAGTTGTGCTTACAGCAGGCAATTTCCAATGTCACCGAATCCAATTAAAGCTCCAAATACTAGTGCAAGTTCAAGTTCAAGTTCCCTCCTGGGCAGAAGAAGCATGTACAGTCAAGGGTTCAACCCATATAGTCATAGGGTTGCCCCAGCAGTTCAAATAAGATCAGTGATTCCTGTTTGTGCAGCACCACCTGTAGCAGTGAGGCCCCCATCAGCAGTAACTCCATCAAACCCGCCACCAGCTTTACCAACATTACCGAGAATGAAAGAAAATACTAACGGTACTACTAGTGCTAGTACTAGTTCACAACCAGCAGCAACAGCAGTGTTGAGTCTTGAAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

491

Amino Acids

53.76

Weight (kDa)

9.79

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 2 - 68 2e-15 Double-stranded RNA binding motif
dsrm PF00035 88 - 152 5.2e-16 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 264, 915
Acc36I ACCTGC 4 cut(s) 57, 279, 952, 1120
AciI CCGC 2 cut(s) 827, 1358
AcsI RAATTY 4 cut(s) 258, 385, 466, 562
AcuI CTGAAG 2 cut(s) 204, 323
AfaI GTAC 5 cut(s) 717, 784, 1232, 1408, 1423
AfiI CCNNNNNNNGG 3 cut(s) 19, 159, 1118
AhlI ACTAGT 4 cut(s) 1052, 1184, 1412, 1424
AjnI CCWGG 3 cut(s) 337, 446, 1212
AjuI GAANNNNNNNTTGG 4 cut(s) 452, 484, 996, 1028
AleI CACNNNNGTG 1 cut(s) 323
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 2 cut(s) 634, 816
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 4 cut(s) 214, 335, 920, 1328
ApeKI GCWGC 9 cut(s) 16, 179, 307, 401, 434, 443, 553, 1307, 1439
ApoI RAATTY 4 cut(s) 258, 385, 466, 562
AspS9I GGNCC 5 cut(s) 77, 215, 225, 335, 1329
AsuHPI GGTGA 3 cut(s) 134, 527, 1149
AsuII TTCGAA 1 cut(s) 1097
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 77, 225
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 990
BbvI GCAGC 9 cut(s) 3, 166, 294, 413, 421, 430, 540, 1319, 1451
BccI CCATC 2 cut(s) 1342, 1357
BciT130I CCWGG 3 cut(s) 339, 448, 1214
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 634, 816
BcuI ACTAGT 4 cut(s) 1052, 1184, 1412, 1424
BfmI CTRYAG 3 cut(s) 17, 725, 1317
BfuAI ACCTGC 4 cut(s) 57, 279, 952, 1120
BglII AGATCT 1 cut(s) 126
BisI GCNGC 9 cut(s) 17, 180, 308, 402, 435, 444, 554, 1308, 1440
BlsI GCNGC 9 cut(s) 18, 181, 309, 403, 436, 445, 555, 1309, 1441
BmcAI AGTACT 2 cut(s) 784, 1423
Bme1390I CCNGG 3 cut(s) 339, 448, 1214
Bme18I GGWCC 2 cut(s) 77, 225
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 5 cut(s) 77, 215, 225, 335, 1329
BmiI GGNNCC 3 cut(s) 768, 1123, 1331
BmrFI CCNGG 3 cut(s) 339, 448, 1214
BmsI GCATC 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 754
Bpu14I TTCGAA 1 cut(s) 1097
BpuEI CTTGAG 2 cut(s) 559, 615
BsaJI CCNNGG 2 cut(s) 338, 1213
BsaXI ACNNNNNCTCC 4 cut(s) 680, 710, 878, 908
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 159, 1118
Bse1I ACTGG 4 cut(s) 259, 737, 909, 1125
BseBI CCWGG 3 cut(s) 339, 448, 1214
BseDI CCNNGG 2 cut(s) 338, 1213
BseGI GGATG 1 cut(s) 1003
BseLI CCNNNNNNNGG 3 cut(s) 19, 159, 1118
BseMII CTCAG 2 cut(s) 149, 414
BseNI ACTGG 4 cut(s) 259, 737, 909, 1125
BseRI GAGGAG 1 cut(s) 619
BseXI GCAGC 9 cut(s) 3, 166, 294, 413, 421, 430, 540, 1319, 1451
BseYI CCCAGC 2 cut(s) 296, 1270
BsgI GTGCAG 2 cut(s) 133, 1326
BshFI GGCC 4 cut(s) 216, 337, 922, 1330
BsiHKAI GWGCWC 1 cut(s) 990
BsiHKCI CYCGRG 1 cut(s) 771
BslFI GGGAC 2 cut(s) 332, 698
BslI CCNNNNNNNGG 3 cut(s) 19, 159, 1118
BsmAI GTCTC 2 cut(s) 634, 816
BsmBI CGTCTC 2 cut(s) 634, 816
BsmFI GGGAC 2 cut(s) 332, 698
BsmI GAATGC 2 cut(s) 172, 436
BsnI GGCC 4 cut(s) 216, 337, 922, 1330
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 1 cut(s) 1097
Bsp1286I GDGCHC 2 cut(s) 140, 990
Bsp1407I TGTACA 1 cut(s) 1230
Bsp143I GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
BspACI CCGC 2 cut(s) 827, 1358
BspANI GGCC 4 cut(s) 216, 337, 922, 1330
BspCNI CTCAG 2 cut(s) 150, 415
BspLI GGNNCC 3 cut(s) 768, 1123, 1331
BspMAI CTGCAG 1 cut(s) 21
BspMI ACCTGC 4 cut(s) 57, 279, 952, 1120
BspQI GCTCTTC 1 cut(s) 978
BspT104I TTCGAA 1 cut(s) 1097
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 4 cut(s) 259, 737, 909, 1125
BssECI CCNNGG 2 cut(s) 338, 1213
BssMI GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
Bst2UI CCWGG 3 cut(s) 339, 448, 1214
Bst4CI ACNGT 6 cut(s) 122, 325, 600, 649, 1235, 1406
Bst6I CTCTTC 2 cut(s) 819, 978
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 1230
BstBI TTCGAA 1 cut(s) 1097
BstC8I GCNNGC 7 cut(s) 27, 165, 375, 379, 420, 537, 1142
BstDEI CTNAG 2 cut(s) 158, 423
BstF5I GGATG 1 cut(s) 1003
BstKTI GATC 7 cut(s) 85, 129, 332, 520, 619, 1038, 1289
BstMAI GTCTC 2 cut(s) 634, 816
BstMBI GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
BstNI CCWGG 3 cut(s) 339, 448, 1214
BstNSI RCATGY 2 cut(s) 347, 1231
BstSCI CCNGG 3 cut(s) 337, 446, 1212
BstSFI CTRYAG 3 cut(s) 17, 725, 1317
BstV1I GCAGC 9 cut(s) 3, 166, 294, 413, 421, 430, 540, 1319, 1451
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 4 cut(s) 216, 337, 922, 1330
BtsCI GGATG 1 cut(s) 1003
BtsI GCAGTG 2 cut(s) 1329, 1455
BtsIMutI CAGTG 5 cut(s) 155, 730, 1296, 1329, 1455
BveI ACCTGC 4 cut(s) 57, 279, 952, 1120
Cac8I GCNNGC 7 cut(s) 27, 165, 375, 379, 420, 537, 1142
CaiI CAGNNNCTG 1 cut(s) 338
Cfr13I GGNCC 5 cut(s) 77, 215, 225, 335, 1329
CseI GACGC 1 cut(s) 798
Csp6I GTAC 5 cut(s) 716, 783, 1231, 1407, 1422
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
CviAII CATG 4 cut(s) 86, 344, 382, 1228
CviQI GTAC 5 cut(s) 716, 783, 1231, 1407, 1422
DdeI CTNAG 2 cut(s) 158, 423
DpnI GATC 7 cut(s) 84, 128, 331, 519, 618, 1037, 1288
DpnII GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
Eam1104I CTCTTC 2 cut(s) 819, 978
EarI CTCTTC 2 cut(s) 819, 978
Eco147I AGGCCT 1 cut(s) 922
Eco24I GRGCYC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 1030
Eco47I GGWCC 2 cut(s) 77, 225
Eco57I CTGAAG 2 cut(s) 204, 323
Eco88I CYCGRG 1 cut(s) 771
EcoO109I RGGNCCY 4 cut(s) 215, 225, 335, 1329
EcoRII CCWGG 3 cut(s) 337, 446, 1212
EcoRV GATATC 1 cut(s) 1030
EcoT38I GRGCYC 1 cut(s) 140
Esp3I CGTCTC 2 cut(s) 634, 816
FaeI CATG 4 cut(s) 89, 347, 385, 1231
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 2 cut(s) 332, 698
FatI CATG 4 cut(s) 85, 343, 381, 1227
FauI CCCGC 1 cut(s) 1365
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 9 cut(s) 17, 180, 308, 402, 435, 444, 554, 1308, 1440
FokI GGATG 1 cut(s) 1010
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 9 cut(s) 17, 180, 308, 402, 435, 444, 554, 1308, 1440
GluI GCNGC 9 cut(s) 17, 180, 308, 402, 435, 444, 554, 1308, 1440
GsaI CCCAGC 2 cut(s) 300, 1274
GsuI CTGGAG 1 cut(s) 754
HaeIII GGCC 4 cut(s) 216, 337, 922, 1330
HgaI GACGC 1 cut(s) 798
Hin1II CATG 4 cut(s) 89, 347, 385, 1231
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 8 cut(s) 96, 570, 721, 842, 1099, 1162, 1294, 1456
HpaI GTTAAC 1 cut(s) 112
HphI GGTGA 3 cut(s) 134, 527, 1149
Hpy166II GTNNAC 3 cut(s) 112, 316, 1430
Hpy188I TCNGA 3 cut(s) 71, 886, 1027
Hpy188III TCNNGA 6 cut(s) 133, 245, 278, 521, 1033, 1460
Hpy8I GTNNAC 3 cut(s) 112, 316, 1430
HpyAV CCTTC 4 cut(s) 63, 68, 228, 494
HpyCH4III ACNGT 6 cut(s) 122, 325, 600, 649, 1235, 1406
HpyF3I CTNAG 2 cut(s) 158, 423
Hsp92II CATG 4 cut(s) 89, 347, 385, 1231
Ksp22I TGATCA 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 3 cut(s) 22, 532, 1180
Lsp1109I GCAGC 9 cut(s) 3, 166, 294, 413, 421, 430, 540, 1319, 1451
LweI GCATC 1 cut(s) 75
MaeIII GTNAC 4 cut(s) 544, 1085, 1155, 1342
MalI GATC 7 cut(s) 84, 128, 331, 519, 618, 1037, 1288
MboI GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
MboII GAAGA 8 cut(s) 121, 439, 596, 806, 809, 995, 1016, 1233
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 140, 990
MluCI AATT 9 cut(s) 258, 385, 438, 466, 562, 889, 904, 1145, 1167
MlyI GAGTC 3 cut(s) 564, 851, 1465
MmeI TCCRAC 1 cut(s) 729
MseI TTAA 4 cut(s) 111, 230, 390, 1170
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 16
MspR9I CCNGG 3 cut(s) 339, 448, 1214
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 2 cut(s) 172, 436
MvaI CCWGG 3 cut(s) 339, 448, 1214
NdeII GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
NlaIII CATG 4 cut(s) 89, 347, 385, 1231
NlaIV GGNNCC 3 cut(s) 768, 1123, 1331
NmuCI GTSAC 2 cut(s) 544, 1155
NspI RCATGY 2 cut(s) 347, 1231
NspV TTCGAA 1 cut(s) 1097
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 771
PceI AGGCCT 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 2 cut(s) 172, 436
PfeI GAWTC 5 cut(s) 96, 721, 1099, 1162, 1294
PkrI GCNGC 9 cut(s) 18, 181, 309, 403, 436, 445, 555, 1309, 1441
PleI GAGTC 3 cut(s) 564, 850, 1464
PpsI GAGTC 3 cut(s) 564, 850, 1464
PpuMI RGGWCCY 1 cut(s) 225
PsiI TTATAA 2 cut(s) 264, 915
Psp5II RGGWCCY 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 337, 446, 1212
PspFI CCCAGC 2 cut(s) 296, 1270
PspGI CCWGG 3 cut(s) 337, 446, 1212
PspN4I GGNNCC 3 cut(s) 768, 1123, 1331
PspPI GGNCC 5 cut(s) 77, 215, 225, 335, 1329
PspPPI RGGWCCY 1 cut(s) 225
PspXI VCTCGAGB 1 cut(s) 771
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 5 cut(s) 717, 784, 1232, 1408, 1423
RsaNI GTAC 5 cut(s) 716, 783, 1231, 1407, 1422
RseI CAYNNNNRTG 1 cut(s) 323
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 4 cut(s) 111, 230, 390, 1170
SatI GCNGC 9 cut(s) 17, 180, 308, 402, 435, 444, 554, 1308, 1440
Sau3AI GATC 7 cut(s) 82, 126, 329, 517, 616, 1035, 1286
Sau96I GGNCC 5 cut(s) 77, 215, 225, 335, 1329
ScaI AGTACT 2 cut(s) 784, 1423
SchI GAGTC 3 cut(s) 564, 851, 1465
ScrFI CCNGG 3 cut(s) 339, 448, 1214
SduI GDGCHC 2 cut(s) 140, 990
SfaNI GCATC 1 cut(s) 75
SfcI CTRYAG 3 cut(s) 17, 725, 1317
Sfr274I CTCGAG 1 cut(s) 771
SfuI TTCGAA 1 cut(s) 1097
SinI GGWCC 2 cut(s) 77, 225
SlaI CTCGAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 574, 630, 771
SmoI CTYRAG 3 cut(s) 574, 630, 771
SpeI ACTAGT 4 cut(s) 1052, 1184, 1412, 1424
Sse9I AATT 9 cut(s) 258, 385, 438, 466, 562, 889, 904, 1145, 1167
SseBI AGGCCT 1 cut(s) 922
SsiI CCGC 2 cut(s) 827, 1358
StuI AGGCCT 1 cut(s) 922
StyD4I CCNGG 3 cut(s) 337, 446, 1212
TaaI ACNGT 6 cut(s) 122, 325, 600, 649, 1235, 1406
TaqI TCGA 3 cut(s) 132, 772, 1097
TasI AATT 9 cut(s) 258, 385, 438, 466, 562, 889, 904, 1145, 1167
TatI WGTACW 4 cut(s) 715, 782, 1230, 1421
TfiI GAWTC 5 cut(s) 96, 721, 1099, 1162, 1294
Tru1I TTAA 4 cut(s) 111, 230, 390, 1170
Tru9I TTAA 4 cut(s) 111, 230, 390, 1170
TscAI CASTG 5 cut(s) 162, 737, 1296, 1329, 1455
TseFI GTSAC 2 cut(s) 544, 1155
TseI GCWGC 9 cut(s) 16, 179, 307, 401, 434, 443, 553, 1307, 1439
Tsp45I GTSAC 2 cut(s) 544, 1155
TspDTI ATGAA 6 cut(s) 45, 264, 398, 668, 762, 1403
TspRI CASTG 5 cut(s) 162, 737, 1296, 1329, 1455
VpaK11BI GGWCC 2 cut(s) 77, 225
XapI RAATTY 4 cut(s) 258, 385, 466, 562
XceI RCATGY 2 cut(s) 347, 1231
XhoI CTCGAG 1 cut(s) 771
ZrmI AGTACT 2 cut(s) 784, 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.