Rorug02G0591100

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006533
Physical Location & Seq
Reverse (-)
71356221 .. 71357069
849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0591100.1

Sequence Viewer

Length: 849 bp
ATGGATGAGATCATTTACAAAAAAAACCAGTTCCAATTCTATCTGAAAGTTAGCTACCTTGAATGTGACGAGACAGAAGTACCTGACTCCGAAGCTCCAGTACCACTTGAGGTTGTGTTCAAGTCCCGTGTCCTTCACTACGCCTTCGATCATTCTTCAGCTACTTTTGATCCCGCTAACGACTTTGTTAGTTGCGATTACAAGAAGACAATATGTGGATGTTGTGATCTTCGCATGTTGTCTTCGTCGACAGAGATTCTTTCTAAGTCGATGTCTGCAGCAAACCCTGACATCAAGCCGGGAAGTGATTTTCACGATGAGATTGTGAATAAAATTGTCAGAGTTGTCAACCACTACTCTGACTCCCAGTACATTGATTGGATCAAGCTATTGAAGGTGAAAGTTGAAATAACTGAACAAGTATTGGATCGCTGCGAGTGCGACGAGTGTAGAGCTACAAGGGAATCGATTTTGATGGCAGAGAGGGAAGCAGAAAAGTTAATAGTACCCGCGAGTGAGTCATCGATCAAGAAGCTACTGAAGAGGGTTAGGGTAGTGGCCGCAGACTTGGAGGAGGGTCATGAAGGTCGTCAAAGAAAAAGAAGAAGAAGTCGTGGCAGTAAGAGTGGTAACAATGTAGTTGCAAGGCCAAGTGATAGCGATTGCTGTATTGTTTGTTCCGATGAGTTTACTGCCGGAAAGTATGCGGTTCAAATGCCATGTTTGCATTCGTTTCATAGATCGTGCATTGAGAAATGGCTGAGACGGAGTCACCATTGCCCTATTTGCCGATATGAGATGCCCGTGGCTGATGTTCAAGAAGATGTAGAGACATTGTTAAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

282

Amino Acids

32.24

Weight (kDa)

5.9

Isoelectric Point (pI)

66.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 219 - 264 3e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 222 - 264 4.6e-12 Ring finger domain
zf-C3HC4_2 PF13923 223 - 263 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 223 - 261 2.5e-07 RING-type zinc-finger
zf-C3HC4 PF00097 223 - 263 2.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 248
AccII CGCG 1 cut(s) 512
AciI CCGC 4 cut(s) 174, 510, 561, 707
AclWI GGATC 3 cut(s) 164, 389, 435
AcoI YGGCCR 1 cut(s) 558
AcuI CTGAAG 2 cut(s) 141, 560
AfaI GTAC 4 cut(s) 81, 102, 371, 507
AgsI TTSAA 6 cut(s) 62, 121, 394, 407, 713, 818
AluBI AGCT 7 cut(s) 54, 95, 161, 388, 455, 535, 846
AluI AGCT 7 cut(s) 54, 95, 161, 388, 455, 535, 846
Alw26I GTCTC 3 cut(s) 65, 757, 824
AlwI GGATC 3 cut(s) 164, 389, 435
AoxI GGCC 2 cut(s) 558, 647
ApeKI GCWGC 3 cut(s) 278, 432, 843
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 2 cut(s) 409, 764
BaeI ACNNNNGTAYC 2 cut(s) 63, 96
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BbsI GAAGAC 2 cut(s) 212, 234
BbvI GCAGC 2 cut(s) 290, 419
BccI CCATC 1 cut(s) 469
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 65, 757, 824
BfaI CTAG 1 cut(s) 847
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 4 cut(s) 279, 433, 561, 844
BlsI GCNGC 4 cut(s) 280, 434, 562, 845
Bme1390I CCNGG 1 cut(s) 300
BmrFI CCNGG 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 1 cut(s) 789
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 2 cut(s) 212, 234
BpmI CTGGAG 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 300
Bsa29I ATCGAT 2 cut(s) 467, 524
BsaJI CCNNGG 1 cut(s) 804
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bse1I ACTGG 3 cut(s) 28, 98, 367
Bse3DI GCAATG 1 cut(s) 775
BseCI ATCGAT 2 cut(s) 467, 524
BseDI CCNNGG 1 cut(s) 804
BseGI GGATG 2 cut(s) 10, 224
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 3 cut(s) 28, 98, 367
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 2 cut(s) 290, 419
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 2 cut(s) 560, 649
BshVI ATCGAT 2 cut(s) 467, 524
BsiSI CCGG 2 cut(s) 299, 696
BslFI GGGAC 1 cut(s) 109
BsmAI GTCTC 3 cut(s) 65, 757, 824
BsmBI CGTCTC 1 cut(s) 757
BsmFI GGGAC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 2 cut(s) 560, 649
Bsp143I GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
BspACI CCGC 4 cut(s) 174, 510, 561, 707
BspANI GGCC 2 cut(s) 560, 649
BspCNI CTCAG 1 cut(s) 753
BspDI ATCGAT 2 cut(s) 467, 524
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 1 cut(s) 580
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 3 cut(s) 164, 389, 435
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 3 cut(s) 28, 98, 367
BssECI CCNNGG 1 cut(s) 804
BssMI GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
Bst6I CTCTTC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 264, 761
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 2 cut(s) 10, 224
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 8 cut(s) 12, 151, 172, 229, 384, 430, 528, 743
BstMAI GTCTC 3 cut(s) 65, 757, 824
BstMBI GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
BstMWI GCNNNNNNNGC 3 cut(s) 438, 724, 786
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 290, 419
BstV2I GAAGAC 2 cut(s) 212, 234
Bsu15I ATCGAT 2 cut(s) 467, 524
BsuRI GGCC 2 cut(s) 560, 649
BsuTUI ATCGAT 2 cut(s) 467, 524
BtgI CCRYGG 1 cut(s) 804
BtsCI GGATG 2 cut(s) 10, 224
CciI TCATGA 1 cut(s) 580
ClaI ATCGAT 2 cut(s) 467, 524
Csp6I GTAC 4 cut(s) 80, 101, 370, 506
CviAII CATG 3 cut(s) 235, 581, 720
CviQI GTAC 4 cut(s) 80, 101, 370, 506
DdeI CTNAG 2 cut(s) 264, 761
DpnI GATC 8 cut(s) 11, 150, 171, 228, 383, 429, 527, 742
DpnII GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
EaeI YGGCCR 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
Eco57I CTGAAG 2 cut(s) 141, 560
Esp3I CGTCTC 1 cut(s) 757
FaeI CATG 3 cut(s) 238, 584, 723
FaiI YATR 7 cut(s) 214, 236, 582, 705, 721, 738, 795
FaqI GGGAC 1 cut(s) 109
FatI CATG 3 cut(s) 234, 580, 719
FauI CCCGC 2 cut(s) 181, 517
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 4 cut(s) 279, 433, 561, 844
FokI GGATG 2 cut(s) 17, 231
Fsp4HI GCNGC 4 cut(s) 279, 433, 561, 844
FspBI CTAG 1 cut(s) 847
GluI GCNGC 4 cut(s) 279, 433, 561, 844
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 560, 649
HapII CCGG 2 cut(s) 299, 696
Hin1II CATG 3 cut(s) 238, 584, 723
HincII GTYRAC 2 cut(s) 249, 349
HindII GTYRAC 2 cut(s) 249, 349
HinfI GANTC 6 cut(s) 86, 256, 362, 464, 518, 769
HpaII CCGG 2 cut(s) 299, 696
HphI GGTGA 2 cut(s) 409, 764
Hpy166II GTNNAC 3 cut(s) 249, 349, 690
Hpy188I TCNGA 5 cut(s) 45, 91, 341, 361, 682
Hpy188III TCNNGA 4 cut(s) 314, 529, 581, 818
Hpy8I GTNNAC 3 cut(s) 249, 349, 690
Hpy99I CGWCG 2 cut(s) 250, 446
HpyAV CCTTC 4 cut(s) 143, 154, 388, 578
HpyCH4V TGCA 4 cut(s) 278, 644, 727, 747
HpyF10VI GCNNNNNNNGC 3 cut(s) 438, 724, 786
HpyF3I CTNAG 2 cut(s) 264, 761
Hsp92II CATG 3 cut(s) 238, 584, 723
Kzo9I GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 7 cut(s) 41, 96, 111, 300, 312, 380, 709
Lsp1109I GCAGC 2 cut(s) 290, 419
LweI GCATC 1 cut(s) 789
MaeI CTAG 1 cut(s) 847
MaeIII GTNAC 3 cut(s) 65, 629, 770
MalI GATC 8 cut(s) 11, 150, 171, 228, 383, 429, 527, 742
MboI GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
MboII GAAGA 8 cut(s) 147, 217, 221, 234, 553, 615, 618, 833
MluCI AATT 2 cut(s) 35, 333
MlyI GAGTC 4 cut(s) 80, 356, 527, 778
MnlI CCTC 5 cut(s) 103, 477, 537, 565, 568
MseI TTAA 2 cut(s) 500, 839
MspI CCGG 2 cut(s) 299, 696
MspR9I CCNGG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 727
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 3 cut(s) 438, 724, 786
NciI CCSGG 1 cut(s) 300
NdeII GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
NlaIII CATG 3 cut(s) 238, 584, 723
NmuCI GTSAC 2 cut(s) 65, 770
NspI RCATGY 1 cut(s) 238
PagI TCATGA 1 cut(s) 580
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 2 cut(s) 256, 464
PflFI GACNNNGTC 1 cut(s) 768
PkrI GCNGC 4 cut(s) 280, 434, 562, 845
PleI GAGTC 4 cut(s) 80, 356, 526, 777
PpsI GAGTC 4 cut(s) 80, 356, 526, 777
PsrI GAACNNNNNNTAC 2 cut(s) 661, 693
PstI CTGCAG 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 768
RsaI GTAC 4 cut(s) 81, 102, 371, 507
RsaNI GTAC 4 cut(s) 80, 101, 370, 506
SalI GTCGAC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 500, 839
SatI GCNGC 4 cut(s) 279, 433, 561, 844
Sau3AI GATC 8 cut(s) 9, 148, 169, 226, 381, 427, 525, 740
SchI GAGTC 4 cut(s) 80, 356, 527, 778
ScrFI CCNGG 1 cut(s) 300
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 2 cut(s) 35, 333
SsiI CCGC 4 cut(s) 174, 510, 561, 707
SspMI CTAG 1 cut(s) 847
StyD4I CCNGG 1 cut(s) 298
TaqI TCGA 5 cut(s) 147, 248, 269, 467, 524
TasI AATT 2 cut(s) 35, 333
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 2 cut(s) 256, 464
Tru1I TTAA 2 cut(s) 500, 839
Tru9I TTAA 2 cut(s) 500, 839
TseFI GTSAC 2 cut(s) 65, 770
TseI GCWGC 3 cut(s) 278, 432, 843
Tsp45I GTSAC 2 cut(s) 65, 770
TspDTI ATGAA 2 cut(s) 597, 725
TspGWI ACGGA 1 cut(s) 781
Tth111I GACNNNGTC 1 cut(s) 768
XceI RCATGY 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.