RchiOBHm_Chr2g0176151

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006533
Physical Location & Seq
Forward (+)
88055582 .. 88058594
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54317

Sequence Viewer

Length: 1473 bp
ATGTATAAGAACCAGCTGCAGGAACTTGCACAGAGAAGCTGTTTCAACCTGCCTTCATATTCTTGCATCAGAGAAGGACCAAATCATGCACCAAGATTCAAAGCTTCTGTTAACTTCAACGGTGAGATCTTCGACAGCCCAAACTACTGCACCACACTGAGACAAGCAGAGCATTCTGCTGCTGAAGTAGCCCTCAATGTTCTTTCCACTAGAGGCCCTTCAAGGTCTTTAACTGCAAGAGTTCTTGATGAAACTGGAATATACAAAAACCTGCTTCAAGAAACTGCTCGCAGAGCTGGGCTGAAGCTGCCTGTATACACTATTGTGAGATCAGGGCCAGGGCATGTACCAACTTTCACTTGTAGTGTAGAGCTTGCTGGCATGAATTTTAATGGGGAGGCAGCAAAGACCAAGAAGCAAGCTGAAAAGAATGCAGCAATTGCTGCCTGGTCTGCCTTGAAAAGAATCTCACTGGTGGCAGACAAAGAAGGAGAGAGTAGTGGTGATCAAGAACAGCAGGAGCAAGCAGTTGTGACAAGGCTGCTCTCAAACAAAGAAGACCATAGCAGACAGTTGAGGAGGAGACCAGATCCTCAAACTCAAGCAAGGAGAAGAACTGTGAGGGGTGCTCATAATCATAGTCATAGTTATAGGTACAACAATGCCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACAAACTCTCTAGTACAGAACCTACAGCATTGGAGGCTCATAGATGATCTGTTAACGGATTTGGTTCCTCGAGTTTCAAGTAATTCACAGAAACAAAGCAGCTTTGCGTCTCTTCTCCCTCCACCGCCACCGCGAAAGGAGTCCAAGATTTTTCCACCAACAGCTACACTCAACAAATCAAACAATTCCTCCTACTACAATTCCAGTTATAATAGGCCTGTTCCTGTCCAAATGAGGGGCGGTGGTAGATCACAGGTTGTTCCACCTCCATTGGAAGACCACCAAAAGGATGAGGAAGAATGGCGTGGTACAAAGTCAGATAGCTTGATCGGTAAGATGCCCAATACTGAAAAGGAAGGTCATAGTCCTAGCCCTAGTAATTATTTTTCAAATCCAAGCAGTCCTGTATTTGGTTCCAGTGTTGCACACATCAGGCAATTTCCTATGTCCCCAAATCCAGTTAAGGCACCAACTACTAGTGCAAGTGCAAGTTCAAGTTCCCTCCTGGGCAGAAGAAGCATGTACACTCAAGGGTTCAATCCTTACGGTCATAGGGTTGCCCCAGCAGTTCATATCAGATCGGTGATTCCTGTTTGTGCAGCACCATCTGTAGCAGTGAGGCCCTTAGTAGTAACTCCATCATACCCACCACCATTGCCAATATTACCAAGAATGAAAGAAACTTGTAGCACCAGTTCCGGTCCTGGTACTAGTGCTAGTACTAGTTCACAACCAGCAGCAGTAGCAGTCCTGAAAGAGAGTGATTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

490

Amino Acids

53.43

Weight (kDa)

9.79

Isoelectric Point (pI)

65.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 2 - 68 6.2e-15 Double-stranded RNA binding motif
dsrm PF00035 88 - 152 1.2e-15 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
Acc36I ACCTGC 2 cut(s) 57, 279
AccB1I GGYRCC 1 cut(s) 1165
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 832
AciI CCGC 3 cut(s) 824, 830, 939
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 2 cut(s) 204, 323
AfaI GTAC 7 cut(s) 348, 656, 714, 1009, 1223, 1408, 1420
AfiI CCNNNNNNNGG 4 cut(s) 19, 934, 985, 1109
AhlI ACTAGT 3 cut(s) 1175, 1409, 1421
AjnI CCWGG 4 cut(s) 337, 446, 1203, 1402
AleI CACNNNNGTG 1 cut(s) 323
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 3 cut(s) 154, 577, 813
AlwI GGATC 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 768
AoxI GGCC 4 cut(s) 214, 335, 914, 1319
ApoI RAATTY 1 cut(s) 385
AspS9I GGNCC 5 cut(s) 77, 215, 335, 1320, 1400
AsuHPI GGTGA 3 cut(s) 134, 515, 1294
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 2 cut(s) 77, 1400
BanI GGYRCC 1 cut(s) 1165
BbsI GAAGAC 2 cut(s) 564, 981
Bbv12I GWGCWC 1 cut(s) 631
BccI CCATC 2 cut(s) 1312, 1345
BciT130I CCWGG 4 cut(s) 339, 448, 1205, 1404
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 3 cut(s) 154, 577, 813
BcuI ACTAGT 3 cut(s) 1175, 1409, 1421
BfaI CTAG 8 cut(s) 210, 710, 1068, 1074, 1176, 1410, 1416, 1422
BfmI CTRYAG 3 cut(s) 17, 722, 1308
BfuAI ACCTGC 2 cut(s) 57, 279
BglII AGATCT 1 cut(s) 126
BmcAI AGTACT 1 cut(s) 1420
Bme1390I CCNGG 4 cut(s) 339, 448, 1205, 1404
Bme18I GGWCC 2 cut(s) 77, 1400
BmeT110I CYCGRG 1 cut(s) 768
BmgT120I GGNCC 5 cut(s) 77, 215, 335, 1320, 1400
BmiI GGNNCC 3 cut(s) 765, 1114, 1167
BmrFI CCNGG 4 cut(s) 339, 448, 1205, 1404
BmsI GCATC 2 cut(s) 75, 1026
BpiI GAAGAC 2 cut(s) 564, 981
BplI GAGNNNNNCTC 2 cut(s) 613, 645
BpuEI CTTGAG 2 cut(s) 585, 1212
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 2 cut(s) 338, 1204
BsaWI WCCGGW 1 cut(s) 1397
BsaXI ACNNNNNCTCC 4 cut(s) 650, 680, 872, 902
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 934, 985, 1109
Bse1I ACTGG 6 cut(s) 259, 477, 903, 1116, 1157, 1392
Bse3DI GCAATG 1 cut(s) 1352
BseBI CCWGG 4 cut(s) 339, 448, 1205, 1404
BseDI CCNNGG 2 cut(s) 338, 1204
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 4 cut(s) 19, 934, 985, 1109
BseMI GCAATG 1 cut(s) 1352
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 6 cut(s) 259, 477, 903, 1116, 1157, 1392
BseRI GAGGAG 2 cut(s) 592, 595
BseYI CCCAGC 2 cut(s) 296, 1261
BsgI GTGCAG 2 cut(s) 133, 1317
Bsh1236I CGCG 1 cut(s) 832
BshFI GGCC 4 cut(s) 216, 337, 916, 1321
BshNI GGYRCC 1 cut(s) 1165
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 1 cut(s) 1398
BslFI GGGAC 1 cut(s) 1132
BslI CCNNNNNNNGG 4 cut(s) 19, 934, 985, 1109
BsmAI GTCTC 3 cut(s) 154, 577, 813
BsmBI CGTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 1132
BsmI GAATGC 2 cut(s) 172, 436
BsnI GGCC 4 cut(s) 216, 337, 916, 1321
Bso31I GGTCTC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 768
Bsp1286I GDGCHC 1 cut(s) 631
Bsp1407I TGTACA 1 cut(s) 1221
Bsp143I GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
BspACI CCGC 3 cut(s) 824, 830, 939
BspANI GGCC 4 cut(s) 216, 337, 916, 1321
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 832
BspLI GGNNCC 3 cut(s) 765, 1114, 1167
BspMAI CTGCAG 1 cut(s) 21
BspMI ACCTGC 2 cut(s) 57, 279
BspPI GGATC 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 1165
BspTNI GGTCTC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 1352
BsrGI TGTACA 1 cut(s) 1221
BsrI ACTGG 6 cut(s) 259, 477, 903, 1116, 1157, 1392
BssECI CCNNGG 2 cut(s) 338, 1204
BssMI GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
BssNAI GTATAC 1 cut(s) 316
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 4 cut(s) 339, 448, 1205, 1404
Bst4CI ACNGT 4 cut(s) 122, 573, 619, 1247
Bst6I CTCTTC 1 cut(s) 816
BstAPI GCANNNNNTGC 2 cut(s) 440, 443
BstAUI TGTACA 1 cut(s) 1221
BstC8I GCNNGC 5 cut(s) 289, 375, 379, 420, 525
BstDEI CTNAG 2 cut(s) 158, 1324
BstF5I GGATG 1 cut(s) 994
BstFNI CGCG 1 cut(s) 832
BstKTI GATC 8 cut(s) 129, 332, 508, 592, 748, 950, 1029, 1280
BstMAI GTCTC 3 cut(s) 154, 577, 813
BstMBI GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
BstMWI GCNNNNNNNGC 9 cut(s) 188, 293, 307, 440, 443, 452, 733, 1215, 1442
BstNI CCWGG 4 cut(s) 339, 448, 1205, 1404
BstNSI RCATGY 2 cut(s) 347, 1222
BstSCI CCNGG 4 cut(s) 337, 446, 1203, 1402
BstSFI CTRYAG 3 cut(s) 17, 722, 1308
BstUI CGCG 1 cut(s) 832
BstV2I GAAGAC 2 cut(s) 564, 981
BstX2I RGATCY 2 cut(s) 126, 589
BstYI RGATCY 2 cut(s) 126, 589
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 4 cut(s) 216, 337, 916, 1321
BtsCI GGATG 1 cut(s) 994
BtsI GCAGTG 1 cut(s) 1320
BtsIMutI CAGTG 4 cut(s) 155, 470, 1123, 1320
BveI ACCTGC 2 cut(s) 57, 279
Cac8I GCNNGC 5 cut(s) 289, 375, 379, 420, 525
Cfr13I GGNCC 5 cut(s) 77, 215, 335, 1320, 1400
CseI GACGC 1 cut(s) 795
Csp6I GTAC 7 cut(s) 347, 655, 713, 1008, 1222, 1407, 1419
CspCI CAANNNNNGTGG 4 cut(s) 951, 986, 1335, 1370
CviAII CATG 5 cut(s) 86, 344, 382, 1219, 1470
CviQI GTAC 7 cut(s) 347, 655, 713, 1008, 1222, 1407, 1419
DdeI CTNAG 2 cut(s) 158, 1324
DpnI GATC 8 cut(s) 128, 331, 507, 591, 747, 949, 1028, 1279
DpnII GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco147I AGGCCT 1 cut(s) 916
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 77, 1400
Eco57I CTGAAG 2 cut(s) 204, 323
Eco88I CYCGRG 1 cut(s) 768
EcoO109I RGGNCCY 2 cut(s) 215, 1320
EcoRII CCWGG 4 cut(s) 337, 446, 1203, 1402
Esp3I CGTCTC 1 cut(s) 813
FaeI CATG 5 cut(s) 89, 347, 385, 1222, 1473
FaqI GGGAC 1 cut(s) 1132
FatI CATG 5 cut(s) 85, 343, 381, 1218, 1469
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 1 cut(s) 315
FokI GGATG 1 cut(s) 1001
FspBI CTAG 8 cut(s) 210, 710, 1068, 1074, 1176, 1410, 1416, 1422
GsaI CCCAGC 2 cut(s) 300, 1265
HaeIII GGCC 4 cut(s) 216, 337, 916, 1321
HapII CCGG 1 cut(s) 1398
HgaI GACGC 1 cut(s) 795
Hin1II CATG 5 cut(s) 89, 347, 385, 1222, 1473
HincII GTYRAC 2 cut(s) 112, 753
HindII GTYRAC 2 cut(s) 112, 753
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 5 cut(s) 96, 465, 839, 1285, 1463
HpaI GTTAAC 2 cut(s) 112, 753
HpaII CCGG 1 cut(s) 1398
HphI GGTGA 3 cut(s) 134, 515, 1294
Hpy166II GTNNAC 5 cut(s) 112, 316, 753, 1224, 1427
Hpy188I TCNGA 3 cut(s) 71, 1018, 1277
Hpy188III TCNNGA 4 cut(s) 245, 278, 509, 1450
Hpy8I GTNNAC 5 cut(s) 112, 316, 753, 1224, 1427
HpyAV CCTTC 5 cut(s) 63, 68, 228, 482, 1049
HpyCH4III ACNGT 4 cut(s) 122, 573, 619, 1247
HpyF10VI GCNNNNNNNGC 9 cut(s) 188, 293, 307, 440, 443, 452, 733, 1215, 1442
HpyF3I CTNAG 2 cut(s) 158, 1324
Hsp92II CATG 5 cut(s) 89, 347, 385, 1222, 1473
Ksp22I TGATCA 1 cut(s) 505
KspAI GTTAAC 2 cut(s) 112, 753
Kzo9I GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
LmnI GCTCC 1 cut(s) 520
LweI GCATC 2 cut(s) 75, 1026
MaeI CTAG 8 cut(s) 210, 710, 1068, 1074, 1176, 1410, 1416, 1422
MaeIII GTNAC 2 cut(s) 532, 1330
MalI GATC 8 cut(s) 128, 331, 507, 591, 747, 949, 1028, 1279
MboI GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
MboII GAAGA 7 cut(s) 121, 569, 624, 803, 986, 1007, 1224
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 2 cut(s) 126, 589
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 7 cut(s) 385, 438, 781, 883, 898, 1078, 1136
MlyI GAGTC 1 cut(s) 848
MseI TTAA 5 cut(s) 111, 230, 390, 752, 1161
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 1 cut(s) 1398
MspR9I CCNGG 4 cut(s) 339, 448, 1205, 1404
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 2 cut(s) 172, 436
MvaI CCWGG 4 cut(s) 339, 448, 1205, 1404
MvnI CGCG 1 cut(s) 832
MwoI GCNNNNNNNGC 9 cut(s) 188, 293, 307, 440, 443, 452, 733, 1215, 1442
NdeII GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
NlaIII CATG 5 cut(s) 89, 347, 385, 1222, 1473
NlaIV GGNNCC 3 cut(s) 765, 1114, 1167
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 2 cut(s) 347, 1222
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 768
PceI AGGCCT 1 cut(s) 916
PctI GAATGC 2 cut(s) 172, 436
PfeI GAWTC 4 cut(s) 96, 465, 1285, 1463
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PsiI TTATAA 1 cut(s) 909
Psp6I CCWGG 4 cut(s) 337, 446, 1203, 1402
PspFI CCCAGC 2 cut(s) 296, 1261
PspGI CCWGG 4 cut(s) 337, 446, 1203, 1402
PspN4I GGNNCC 3 cut(s) 765, 1114, 1167
PspPI GGNCC 5 cut(s) 77, 215, 335, 1320, 1400
PspXI VCTCGAGB 1 cut(s) 768
PsrI GAACNNNNNNTAC 2 cut(s) 1378, 1410
PstI CTGCAG 1 cut(s) 21
PsuI RGATCY 2 cut(s) 126, 589
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 7 cut(s) 348, 656, 714, 1009, 1223, 1408, 1420
RsaNI GTAC 7 cut(s) 347, 655, 713, 1008, 1222, 1407, 1419
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 5 cut(s) 111, 230, 390, 752, 1161
Sau3AI GATC 8 cut(s) 126, 329, 505, 589, 745, 947, 1026, 1277
Sau96I GGNCC 5 cut(s) 77, 215, 335, 1320, 1400
ScaI AGTACT 1 cut(s) 1420
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 4 cut(s) 339, 448, 1205, 1404
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 2 cut(s) 75, 1026
SfcI CTRYAG 3 cut(s) 17, 722, 1308
Sfr274I CTCGAG 1 cut(s) 768
SinI GGWCC 2 cut(s) 77, 1400
SlaI CTCGAG 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 600, 768, 1227
SmoI CTYRAG 3 cut(s) 600, 768, 1227
SpeI ACTAGT 3 cut(s) 1175, 1409, 1421
Sse9I AATT 7 cut(s) 385, 438, 781, 883, 898, 1078, 1136
SseBI AGGCCT 1 cut(s) 916
SsiI CCGC 3 cut(s) 824, 830, 939
SspI AATATT 1 cut(s) 1362
SspMI CTAG 8 cut(s) 210, 710, 1068, 1074, 1176, 1410, 1416, 1422
StuI AGGCCT 1 cut(s) 916
StyD4I CCNGG 4 cut(s) 337, 446, 1203, 1402
TaaI ACNGT 4 cut(s) 122, 573, 619, 1247
TaqI TCGA 2 cut(s) 132, 769
TasI AATT 7 cut(s) 385, 438, 781, 883, 898, 1078, 1136
TatI WGTACW 3 cut(s) 712, 1221, 1418
TfiI GAWTC 4 cut(s) 96, 465, 1285, 1463
Tru1I TTAA 5 cut(s) 111, 230, 390, 752, 1161
Tru9I TTAA 5 cut(s) 111, 230, 390, 752, 1161
TscAI CASTG 4 cut(s) 162, 477, 1123, 1320
TseFI GTSAC 1 cut(s) 532
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 5 cut(s) 45, 264, 398, 1259, 1388
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 4 cut(s) 162, 477, 1123, 1320
VpaK11BI GGWCC 2 cut(s) 77, 1400
XapI RAATTY 1 cut(s) 385
XceI RCATGY 2 cut(s) 347, 1222
XhoI CTCGAG 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 8 cut(s) 210, 710, 1068, 1074, 1176, 1410, 1416, 1422
ZrmI AGTACT 1 cut(s) 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.