FvH4_6g12851

Protein NRT1 PTR FAMILY 2.8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003229
Physical Location & Seq
Reverse (-)
7791481 .. 7793475
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12851.t1

Sequence Viewer

Length: 1608 bp
ATGAGTTTGGTGGCAAATCTGGTGCTGTATCTGCATACCAAGTATAACTTGGAAAATGTAGATTCAGCTAATGTATACAATATCTGGTCTGGTTCATGCAACGTTGCACCTCTAATTGGTGCTTACCTTGCTGATAGTCACTTGGGGAAGTTCTCCACTCTTCTATATAGCTCTATAGCATCCCTCATGGGTATGGTGGCCTTGACATTAACTGCAAGTTTACATGAACTAACTCCTCCTGCCTGCAATGGACAAGCGCATTGCGAACAACCCAACGCATGGCAGAAGTTGGTCCTCTATTCTGGTCTGGGATTGCTCGTTATTGGATCTGGAGGCCTCAGACCATGCAACATTGCCTTTGGAGCTGATCAATTTGACACCACAACGGAGAAGGGAAGAGCGCAGCTAGATAGCTTTTGCAATTGGTGGTACTTTTTGTTCACCCTTGCTCTCATCGTAGCTCTGACCGGAGTGGTCTACATACAAACTAATATCAGCTGGGTTCTGGGTTTTGCTATACCAGCTGGATGCTTTGCCTTGTCCATACTGATATTCTTGCTTGGCCGAAAATTGTACGTTCGTGTTAAGCCTCAAGGAAGCACCTTTGGGGACATAATGAAGGTAATTGTAGCTTCTTTTCGTAAGTTTAGTACAAGGACTTCAGGGCAGTCCCTTTATGATGCTCCTTTAATTGGACAAGAGTCGGAGCAAATGCTTGCTCACACAGAGAGGTTCAAGTTTCTTGATAAGGCTGCTCTAATTGTAGATCCAGATGAATTGGATAGCAGTGGCAACCCTAGAACTGGTTGGAGACTCTGCAGTGTGCAACAAGTTGAGCAACTCAAATGCGTCATATCTTTGTTCCCTGTTTGGGTAAGTGGAATTGGTGCTTTCATAGGAATGCAGCAAATGCCATCATTTGGGACCTTTCAAGCGATCCAAACAAACAGAAAGATTGGAAACAATTTCGAGATGCCACCAGCCTGGATTGGTCTCGTACCGATGATTGCACTTTCGATATGGATCATCATCTACGAAAGCATGTACATTCCCTGGATTAAGAAAAGGAAAAACGATGAGACCGGAAGATTGACAATGGAGACAAGATTCAGGGTTGGGATTGTGATGTCAATTCTGTGCATGGTGGTGGCTGGACTCACCGAAATGAAGCGCAGAAAGTCTGCTTTGGAAAACAGATCTTTCCAGTCACCTATAACTGTTGCATTGCTTGTGCCACAGTTTGCCTTGGCAGGTTTGATCGAGGCATTTGCAGCCATTGCCTTATTGGAGTTGCTTACAACACAATGGCCAAAGAGCATGAGGACTTTTGCCGGAGCGGCCTTCTTTCTCAGCTTATCCATTGCAAGCTACGGCACCTCCATTATTGTTACCATTGTCAAGAAAGTGACTAGTTTGAATGGCAATACATCATGGCTGGGTGGTAATGACCTGAACAAGAACAGGCTTGATTACTACTATTACATCATTGCTGCTCTTGGGATCTTGAACTTGTTGTTTTTCGAATTCATTGCACGCCATTTTCTGTCCGATGTCGATGGTCATGACGGTTCTAGAATCCCATCTACTTGTGATGAAGAAAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04974 map04974
Pfam Domains
Protein Families
PTR2

Protein Analysis

536

Amino Acids

59.19

Weight (kDa)

8.56

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 51 - 479 7.8e-73 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1241
AccB1I GGYRCC 1 cut(s) 1373
AccB7I CCANNNNNTGG 2 cut(s) 279, 920
AccBSI CCGCTC 1 cut(s) 1337
AccI GTMKAC 2 cut(s) 75, 477
AciI CCGC 1 cut(s) 1337
AclI AACGTT 1 cut(s) 102
AclWI GGATC 5 cut(s) 334, 761, 931, 1031, 1508
AcoI YGGCCR 2 cut(s) 562, 1307
AcsI RAATTY 1 cut(s) 1523
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 5 cut(s) 431, 575, 652, 999, 1046
AfiI CCNNNNNNNGG 6 cut(s) 116, 279, 692, 803, 871, 920
AgsI TTSAA 4 cut(s) 736, 932, 1417, 1507
AhlI ACTAGT 1 cut(s) 1409
AjnI CCWGG 2 cut(s) 983, 1052
Alw26I GTCTC 4 cut(s) 805, 998, 1073, 1094
AlwI GGATC 5 cut(s) 334, 761, 931, 1031, 1508
AoxI GGCC 5 cut(s) 198, 334, 562, 1307, 1338
ApeKI GCWGC 5 cut(s) 403, 752, 904, 1271, 1490
ApoI RAATTY 1 cut(s) 1523
AspLEI GCGC 3 cut(s) 259, 403, 1173
AspS9I GGNCC 2 cut(s) 292, 924
AsuHPI GGTGA 3 cut(s) 433, 1150, 1200
AsuII TTCGAA 1 cut(s) 1521
AvaII GGWCC 2 cut(s) 292, 924
BalI TGGCCA 1 cut(s) 1309
BanI GGYRCC 1 cut(s) 1373
BarI GAAGNNNNNNTAC 2 cut(s) 643, 675
BbvI GCAGC 5 cut(s) 415, 739, 916, 1283, 1477
BccI CCATC 3 cut(s) 922, 1550, 1588
BceAI ACGGC 1 cut(s) 1387
BcgI CGANNNNNNTGC 4 cut(s) 1250, 1284, 1511, 1545
BciT130I CCWGG 2 cut(s) 985, 1054
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 4 cut(s) 805, 998, 1073, 1094
BcuI ACTAGT 1 cut(s) 1409
BfaI CTAG 4 cut(s) 407, 798, 1410, 1572
BfmI CTRYAG 2 cut(s) 174, 817
BfuAI ACCTGC 1 cut(s) 1241
BglI GCCNNNNNGGC 1 cut(s) 1337
BglII AGATCT 1 cut(s) 1196
BisI GCNGC 6 cut(s) 404, 753, 905, 1272, 1338, 1491
BlsI GCNGC 6 cut(s) 405, 754, 906, 1273, 1339, 1492
Bme1390I CCNGG 2 cut(s) 985, 1054
Bme18I GGWCC 2 cut(s) 292, 924
BmgT120I GGNCC 2 cut(s) 292, 924
BmiI GGNNCC 2 cut(s) 925, 1375
BmrFI CCNGG 2 cut(s) 985, 1054
BmsI GCATC 4 cut(s) 188, 518, 670, 963
BoxI GACNNNNGTC 1 cut(s) 700
BpmI CTGGAG 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 1521
BpuEI CTTGAG 1 cut(s) 576
BsaBI GATNNNNATC 2 cut(s) 1022, 1028
BsaI GGTCTC 2 cut(s) 998, 1073
BsaJI CCNNGG 2 cut(s) 1052, 1245
BsaWI WCCGGW 2 cut(s) 467, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 1361, 1391
Bsc4I CCNNNNNNNGG 6 cut(s) 116, 279, 692, 803, 871, 920
Bse1I ACTGG 2 cut(s) 808, 1204
Bse3DI GCAATG 8 cut(s) 253, 259, 351, 1223, 1275, 1359, 1485, 1527
Bse8I GATNNNNATC 2 cut(s) 1022, 1028
BseBI CCWGG 2 cut(s) 985, 1054
BseDI CCNNGG 2 cut(s) 1052, 1245
BseGI GGATG 2 cut(s) 179, 533
BseJI GATNNNNATC 2 cut(s) 1022, 1028
BseLI CCNNNNNNNGG 6 cut(s) 116, 279, 692, 803, 871, 920
BseMI GCAATG 8 cut(s) 253, 259, 351, 1223, 1275, 1359, 1485, 1527
BseMII CTCAG 2 cut(s) 352, 1363
BseNI ACTGG 2 cut(s) 808, 1204
BseRI GAGGAG 1 cut(s) 225
BseXI GCAGC 5 cut(s) 415, 739, 916, 1283, 1477
BseYI CCCAGC 2 cut(s) 498, 1435
BshFI GGCC 5 cut(s) 200, 336, 564, 1309, 1340
BshNI GGYRCC 1 cut(s) 1373
BsiSI CCGG 3 cut(s) 468, 1083, 1332
BslFI GGGAC 3 cut(s) 623, 655, 937
BslI CCNNNNNNNGG 6 cut(s) 116, 279, 692, 803, 871, 920
BsmAI GTCTC 4 cut(s) 805, 998, 1073, 1094
BsmFI GGGAC 3 cut(s) 623, 655, 937
BsmI GAATGC 1 cut(s) 906
BsnI GGCC 5 cut(s) 200, 336, 564, 1309, 1340
Bso31I GGTCTC 2 cut(s) 998, 1073
Bsp119I TTCGAA 1 cut(s) 1521
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
BspACI CCGC 1 cut(s) 1337
BspANI GGCC 5 cut(s) 200, 336, 564, 1309, 1340
BspCNI CTCAG 2 cut(s) 351, 1362
BspHI TCATGA 1 cut(s) 1561
BspLI GGNNCC 2 cut(s) 925, 1375
BspMAI CTGCAG 1 cut(s) 821
BspMI ACCTGC 1 cut(s) 1241
BspPI GGATC 5 cut(s) 334, 761, 931, 1031, 1508
BspQI GCTCTTC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 1521
BspT107I GGYRCC 1 cut(s) 1373
BspTNI GGTCTC 2 cut(s) 998, 1073
BsrBI CCGCTC 1 cut(s) 1337
BsrDI GCAATG 8 cut(s) 253, 259, 351, 1223, 1275, 1359, 1485, 1527
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 2 cut(s) 808, 1204
BssECI CCNNGG 2 cut(s) 1052, 1245
BssMI GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
BssNAI GTATAC 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 1245
Bst1107I GTATAC 1 cut(s) 76
Bst2UI CCWGG 2 cut(s) 985, 1054
Bst4CI ACNGT 4 cut(s) 1219, 1239, 1568, 1605
Bst6I CTCTTC 2 cut(s) 165, 391
BstAPI GCANNNNNTGC 2 cut(s) 910, 1277
BstAUI TGTACA 1 cut(s) 1044
BstBI TTCGAA 1 cut(s) 1521
BstC8I GCNNGC 4 cut(s) 244, 717, 1366, 1534
BstDEI CTNAG 2 cut(s) 338, 1349
BstF5I GGATG 2 cut(s) 179, 533
BstHHI GCGC 3 cut(s) 259, 403, 1173
BstKTI GATC 8 cut(s) 329, 370, 769, 939, 1026, 1199, 1260, 1503
BstMAI GTCTC 4 cut(s) 805, 998, 1073, 1094
BstMBI GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
BstMWI GCNNNNNNNGC 8 cut(s) 31, 128, 362, 521, 910, 1271, 1277, 1337
BstNI CCWGG 2 cut(s) 985, 1054
BstNSI RCATGY 1 cut(s) 1045
BstPAI GACNNNNGTC 1 cut(s) 700
BstSCI CCNGG 2 cut(s) 983, 1052
BstSFI CTRYAG 2 cut(s) 174, 817
BstV1I GCAGC 5 cut(s) 415, 739, 916, 1283, 1477
BstX2I RGATCY 4 cut(s) 326, 766, 1196, 1500
BstXI CCANNNNNNTGG 1 cut(s) 984
BstYI RGATCY 4 cut(s) 326, 766, 1196, 1500
BstZ17I GTATAC 1 cut(s) 76
BsuRI GGCC 5 cut(s) 200, 336, 564, 1309, 1340
BtsCI GGATG 2 cut(s) 179, 533
BtsI GCAGTG 2 cut(s) 793, 826
BtsIMutI CAGTG 2 cut(s) 793, 826
BveI ACCTGC 1 cut(s) 1241
Cac8I GCNNGC 4 cut(s) 244, 717, 1366, 1534
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 3 cut(s) 259, 403, 1173
Cfr13I GGNCC 2 cut(s) 292, 924
CseI GACGC 1 cut(s) 838
Csp6I GTAC 5 cut(s) 430, 574, 651, 998, 1045
CviQI GTAC 5 cut(s) 430, 574, 651, 998, 1045
DdeI CTNAG 2 cut(s) 338, 1349
DpnI GATC 8 cut(s) 328, 369, 768, 938, 1025, 1198, 1259, 1502
DpnII GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
EaeI YGGCCR 2 cut(s) 562, 1307
Eam1104I CTCTTC 2 cut(s) 165, 391
EarI CTCTTC 2 cut(s) 165, 391
Eco130I CCWWGG 1 cut(s) 1245
Eco147I AGGCCT 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 998, 1073
Eco47I GGWCC 2 cut(s) 292, 924
Eco57I CTGAAG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 924
EcoRI GAATTC 1 cut(s) 1523
EcoRII CCWGG 2 cut(s) 983, 1052
EcoT14I CCWWGG 1 cut(s) 1245
ErhI CCWWGG 1 cut(s) 1245
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 3 cut(s) 623, 655, 937
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 2 cut(s) 75, 477
Fnu4HI GCNGC 6 cut(s) 404, 753, 905, 1272, 1338, 1491
FokI GGATG 2 cut(s) 166, 540
Fsp4HI GCNGC 6 cut(s) 404, 753, 905, 1272, 1338, 1491
FspBI CTAG 4 cut(s) 407, 798, 1410, 1572
GlaI GCGC 3 cut(s) 258, 402, 1172
GluI GCNGC 6 cut(s) 404, 753, 905, 1272, 1338, 1491
GsaI CCCAGC 2 cut(s) 502, 1439
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 5 cut(s) 200, 336, 564, 1309, 1340
HapII CCGG 3 cut(s) 468, 1083, 1332
HgaI GACGC 1 cut(s) 838
HhaI GCGC 3 cut(s) 259, 403, 1173
Hin6I GCGC 3 cut(s) 257, 401, 1171
HinP1I GCGC 3 cut(s) 257, 401, 1171
HinfI GANTC 6 cut(s) 62, 701, 813, 1107, 1155, 1575
HpaII CCGG 3 cut(s) 468, 1083, 1332
HphI GGTGA 3 cut(s) 433, 1150, 1200
Hpy166II GTNNAC 4 cut(s) 76, 221, 441, 478
Hpy188I TCNGA 4 cut(s) 341, 465, 706, 1549
Hpy188III TCNNGA 8 cut(s) 330, 743, 770, 970, 1399, 1504, 1562, 1572
Hpy8I GTNNAC 4 cut(s) 76, 221, 441, 478
HpyAV CCTTC 3 cut(s) 385, 613, 1351
HpyCH4III ACNGT 4 cut(s) 1219, 1239, 1568, 1605
HpyCH4IV ACGT 2 cut(s) 102, 576
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 128, 362, 521, 910, 1271, 1277, 1337
HpyF3I CTNAG 2 cut(s) 338, 1349
HpySE526I ACGT 2 cut(s) 102, 576
HspAI GCGC 3 cut(s) 257, 401, 1171
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 4 cut(s) 362, 688, 706, 1334
Lsp1109I GCAGC 5 cut(s) 415, 739, 916, 1283, 1477
LweI GCATC 4 cut(s) 188, 518, 670, 963
MaeI CTAG 4 cut(s) 407, 798, 1410, 1572
MaeII ACGT 2 cut(s) 102, 576
MaeIII GTNAC 4 cut(s) 137, 1206, 1387, 1405
MalI GATC 8 cut(s) 328, 369, 768, 938, 1025, 1198, 1259, 1502
MbiI CCGCTC 1 cut(s) 1337
MboI GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
MboII GAAGA 4 cut(s) 152, 408, 1098, 1607
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 4 cut(s) 326, 766, 1196, 1500
MlsI TGGCCA 1 cut(s) 1309
MluNI TGGCCA 1 cut(s) 1309
MlyI GAGTC 3 cut(s) 710, 807, 1149
MmeI TCCRAC 2 cut(s) 684, 788
Mox20I TGGCCA 1 cut(s) 1309
MscI TGGCCA 1 cut(s) 1309
MseI TTAA 4 cut(s) 209, 585, 689, 1059
MslI CAYNNNNRTG 4 cut(s) 191, 899, 1145, 1163
Msp20I TGGCCA 1 cut(s) 1309
MspA1I CMGCKG 2 cut(s) 498, 524
MspI CCGG 3 cut(s) 468, 1083, 1332
MspR9I CCNGG 2 cut(s) 985, 1054
MunI CAATTG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 906
MvaI CCWGG 2 cut(s) 985, 1054
MwoI GCNNNNNNNGC 8 cut(s) 31, 128, 362, 521, 910, 1271, 1277, 1337
NdeII GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
NlaIV GGNNCC 2 cut(s) 925, 1375
NmuCI GTSAC 3 cut(s) 137, 1206, 1405
NspI RCATGY 1 cut(s) 1045
NspV TTCGAA 1 cut(s) 1521
PagI TCATGA 1 cut(s) 1561
PceI AGGCCT 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 391
PctI GAATGC 1 cut(s) 906
PfeI GAWTC 3 cut(s) 62, 1107, 1575
PflMI CCANNNNNTGG 2 cut(s) 279, 920
PkrI GCNGC 6 cut(s) 405, 754, 906, 1273, 1339, 1492
PleI GAGTC 3 cut(s) 709, 807, 1149
PpsI GAGTC 3 cut(s) 709, 807, 1149
PpuMI RGGWCCY 1 cut(s) 924
PshAI GACNNNNGTC 1 cut(s) 700
Psp1406I AACGTT 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 983, 1052
PspFI CCCAGC 2 cut(s) 498, 1435
PspGI CCWGG 2 cut(s) 983, 1052
PspN4I GGNNCC 2 cut(s) 925, 1375
PspPI GGNCC 2 cut(s) 292, 924
PspPPI RGGWCCY 1 cut(s) 924
PsrI GAACNNNNNNTAC 2 cut(s) 422, 454
PstI CTGCAG 1 cut(s) 821
PsuI RGATCY 4 cut(s) 326, 766, 1196, 1500
PvuII CAGCTG 2 cut(s) 498, 524
RsaI GTAC 5 cut(s) 431, 575, 652, 999, 1046
RsaNI GTAC 5 cut(s) 430, 574, 651, 998, 1045
RseI CAYNNNNRTG 4 cut(s) 191, 899, 1145, 1163
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 4 cut(s) 209, 585, 689, 1059
SatI GCNGC 6 cut(s) 404, 753, 905, 1272, 1338, 1491
Sau3AI GATC 8 cut(s) 326, 367, 766, 936, 1023, 1196, 1257, 1500
Sau96I GGNCC 2 cut(s) 292, 924
SchI GAGTC 3 cut(s) 710, 807, 1149
ScrFI CCNGG 2 cut(s) 985, 1054
SfaNI GCATC 4 cut(s) 188, 518, 670, 963
SfcI CTRYAG 2 cut(s) 174, 817
SfuI TTCGAA 1 cut(s) 1521
SinI GGWCC 2 cut(s) 292, 924
SmiMI CAYNNNNRTG 4 cut(s) 191, 899, 1145, 1163
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
SpeI ACTAGT 1 cut(s) 1409
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 1 cut(s) 1337
SspMI CTAG 4 cut(s) 407, 798, 1410, 1572
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 983, 1052
StyI CCWWGG 1 cut(s) 1245
TaaI ACNGT 4 cut(s) 1219, 1239, 1568, 1605
TaiI ACGT 2 cut(s) 105, 579
TaqI TCGA 5 cut(s) 969, 1016, 1260, 1521, 1554
TatI WGTACW 2 cut(s) 650, 1044
TauI GCSGC 1 cut(s) 1340
TfiI GAWTC 3 cut(s) 62, 1107, 1575
Tru1I TTAA 4 cut(s) 209, 585, 689, 1059
Tru9I TTAA 4 cut(s) 209, 585, 689, 1059
TscAI CASTG 2 cut(s) 793, 826
TseFI GTSAC 3 cut(s) 137, 1206, 1405
TseI GCWGC 5 cut(s) 403, 752, 904, 1271, 1490
Tsp45I GTSAC 3 cut(s) 137, 1206, 1405
TspDTI ATGAA 8 cut(s) 84, 240, 632, 789, 883, 1181, 1516, 1608
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 793, 826
Van91I CCANNNNNTGG 2 cut(s) 279, 920
VpaK11BI GGWCC 2 cut(s) 292, 924
XapI RAATTY 1 cut(s) 1523
XbaI TCTAGA 1 cut(s) 1571
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 1282
XmiI GTMKAC 2 cut(s) 75, 477
XspI CTAG 4 cut(s) 407, 798, 1410, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.