RLG00000024668

Protein NRT1 PTR FAMILY 2.8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003229
Physical Location & Seq
Forward (+)
38027777 .. 38029703
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024668

Sequence Viewer

Length: 1695 bp
ATGGAGCATGCGACAATGCATTCTTCATCCCCTCCCAAACAACCAGTCGGAGGGTGGAAAGCCGTCAGATACATCCTTGGGAATGAGACCTTTGAGAAATTGGCTTCCATGAGTTTGGTGGCAAATCTGGTGCTGTATCTGCATACCAAGTATAACTTGGATAATGTAGTTTCAGCTAATGTATACAATATCTGGTCTGGTTCATGCAATGTTGCACCCTTAATTGGTGCCTACCTTGCTGATACTCTTTTGGGAAAGTACTCTACTCTTCTTTATAGCTCAATAGCATCCCTCATGGGTATGGTGTCCTTGACATTAACTGCAAGTTTACATGAGCTAACTCCTCCTGCCTGCAATGGACAAGCGCAATGCCAGCAACCCAATGCATGGCAGAAGTTTGTCCTCTTTTCGGGTCTGGGACTCCTTGTTATTGGATCTGGAGGCCTCAGACCATGCAACATTGCCTTTGGAGCTGATCAATTCGACACTACAACAGGGAAGGGAAGAGCACAGCTAGATAGCTTTTGCAATTGGTGGTACTTTTTGTTCACCATTGCTCTCATTGTAGCTCTGACCGGAGTGGTCTACATACAAACTCATATCAGCTGGGTTTTGGGTTTTGCTATACCAGCTGGATGCTTTGCCCTCTCCATACTGATTTTCTTGCTCGGCCGAAAATTGTACATTCGTGTTAAGCCTCAAGGAAGCACCTTTGGGGACATAATGAGGGTAATTGTAGCTGCTTTTCGTAAGTTTAGAACAAAGACTTCAGGGCAGTCCCTTTATGATGCTCCTTCAATTGGACAAGAGTTGGAGCTAACACTTGCTCACACAGAGAGGTTCGAGTTTCTTGATAAGGCTGCTGTACTTGTAGATCCAAATGAATTGGATAGCAATGGCAACCCTAAAAGCGGTTGGAGACTATGCAGTGTGCCACAAGTTGAGCGACTCAAATACGTCATAGCTTTGTTACCTGTTTGTATAAGTGGAATTGGTTGTTTCATAGGAATGCAGCAAATGCCATCATTTGGGACCTTTCAAGCTATTCAAACAAACAAAAAGATTGGAAACAATTTTGAAATCCCACCAGCCTGGATGGGTCTCACACAAATGATTGCACTTTCAATATGGATCATCATCTACGAATGCATTTACATCCCGTGGATTCAGAAAAGGAACAATGATGAATCTGGAAGATTGACAATGGAGACGAGATTCAAGGTTGGCATTGTGATGTCAATTCTGTGCATGGTGGTGGCTGGACTCACCGAAATGAAGCGCAGAAACTCTGCTTTGGAAAACAGATCTTTCGAGTCACCTATAACTGTTGCATTGCTTGTGCCACAATTTGCCTTGTCAGGTTTGATCGAGGCATTTGCAGCCATTGCCTTAATGGAGTTGCTTACAACACAGTGGCCAAAGAGCATGAGGACTTTTGCCGGGGCGGTCTTCTTTCTCAGCTTATCCATTGCAAGCTACTTCACCTCCATTCTTGTTAGCGTCGTCAAGAAAGTGACTAGTTTGAATGGCAATACATCATGGCTGGGTGGTAATGACCTAAACAAGAACAGGCTTGATTACTACTATTACACCATTGCTGGTCTCGGGGTCTTGAACTTGTTGTATTTCCAATTCATTGCACGCCATTTTCTGTCCAATGCCGATGGTCATGACCGTTCTAGAATCCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04974 map04974
Pfam Domains
Protein Families
PTR2

Protein Analysis

565

Amino Acids

62.37

Weight (kDa)

9.04

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 87 - 515 7.3e-69 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 2 cut(s) 387, 1028
AccI GTMKAC 2 cut(s) 183, 585
AciI CCGC 2 cut(s) 912, 1445
AclWI GGATC 3 cut(s) 442, 869, 1139
AcoI YGGCCR 2 cut(s) 670, 1415
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 4 cut(s) 260, 539, 683, 867
AfiI CCNNNNNNNGG 7 cut(s) 50, 224, 387, 409, 800, 911, 1028
AgsI TTSAA 8 cut(s) 798, 1040, 1049, 1079, 1125, 1219, 1525, 1615
AhlI ACTAGT 1 cut(s) 1517
AjnI CCWGG 1 cut(s) 1091
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 5 cut(s) 80, 913, 1106, 1202, 1607
AlwI GGATC 3 cut(s) 442, 869, 1139
Ama87I CYCGRG 1 cut(s) 1604
AoxI GGCC 3 cut(s) 442, 670, 1415
ApeKI GCWGC 4 cut(s) 740, 860, 1012, 1379
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspLEI GCGC 2 cut(s) 367, 1281
AspS9I GGNCC 1 cut(s) 1032
AsuC2I CCSGG 1 cut(s) 1441
AsuHPI GGTGA 4 cut(s) 541, 1258, 1308, 1474
AvaI CYCGRG 1 cut(s) 1604
AvaII GGWCC 1 cut(s) 1032
BalI TGGCCA 1 cut(s) 1417
BanI GGYRCC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 1441
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 4 cut(s) 727, 847, 1024, 1391
BccI CCATC 3 cut(s) 1030, 1090, 1658
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 1 cut(s) 1093
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 1441
BcoDI GTCTC 5 cut(s) 80, 913, 1106, 1202, 1607
BcuI ACTAGT 1 cut(s) 1517
BfaI CTAG 4 cut(s) 515, 1518, 1680, 1693
BglII AGATCT 1 cut(s) 1304
BisI GCNGC 4 cut(s) 741, 861, 1013, 1380
BlsI GCNGC 4 cut(s) 742, 862, 1014, 1381
BmcAI AGTACT 1 cut(s) 260
Bme1390I CCNGG 2 cut(s) 1093, 1441
Bme18I GGWCC 1 cut(s) 1032
BmeT110I CYCGRG 1 cut(s) 1604
BmgT120I GGNCC 1 cut(s) 1032
BmiI GGNNCC 2 cut(s) 229, 1033
BmrFI CCNGG 2 cut(s) 1093, 1441
BmsI GCATC 3 cut(s) 296, 626, 778
BpiI GAAGAC 1 cut(s) 1441
BpmI CTGGAG 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 684
BpuMI CCSGG 1 cut(s) 1441
BsaBI GATNNNNATC 1 cut(s) 1136
BsaI GGTCTC 3 cut(s) 80, 1106, 1607
BsaJI CCNNGG 3 cut(s) 76, 1160, 1440
BsaWI WCCGGW 1 cut(s) 575
BsaXI ACNNNNNCTCC 2 cut(s) 1469, 1499
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 224, 387, 409, 800, 911, 1028
Bse1I ACTGG 1 cut(s) 44
Bse8I GATNNNNATC 1 cut(s) 1136
BseBI CCWGG 1 cut(s) 1093
BseDI CCNNGG 3 cut(s) 76, 1160, 1440
BseGI GGATG 6 cut(s) 26, 72, 287, 641, 1101, 1155
BseJI GATNNNNATC 1 cut(s) 1136
BseLI CCNNNNNNNGG 7 cut(s) 50, 224, 387, 409, 800, 911, 1028
BseMII CTCAG 2 cut(s) 460, 1471
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 670
BseXI GCAGC 4 cut(s) 727, 847, 1024, 1391
BseYI CCCAGC 2 cut(s) 606, 1543
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 3 cut(s) 444, 672, 1417
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 673
BsiHKAI GWGCWC 1 cut(s) 511
BsiHKCI CYCGRG 1 cut(s) 1604
BsiSI CCGG 2 cut(s) 576, 1440
BslFI GGGAC 4 cut(s) 432, 731, 763, 1045
BslI CCNNNNNNNGG 7 cut(s) 50, 224, 387, 409, 800, 911, 1028
BsmAI GTCTC 5 cut(s) 80, 913, 1106, 1202, 1607
BsmBI CGTCTC 1 cut(s) 1202
BsmFI GGGAC 4 cut(s) 432, 731, 763, 1045
BsmI GAATGC 3 cut(s) 19, 1014, 1151
BsnI GGCC 3 cut(s) 444, 672, 1417
Bso31I GGTCTC 3 cut(s) 80, 1106, 1607
BsoBI CYCGRG 1 cut(s) 1604
Bsp1286I GDGCHC 1 cut(s) 511
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
BspACI CCGC 2 cut(s) 912, 1445
BspANI GGCC 3 cut(s) 444, 672, 1417
BspCNI CTCAG 2 cut(s) 459, 1470
BspHI TCATGA 1 cut(s) 1669
BspLI GGNNCC 2 cut(s) 229, 1033
BspPI GGATC 3 cut(s) 442, 869, 1139
BspQI GCTCTTC 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 3 cut(s) 80, 1106, 1607
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 3 cut(s) 76, 1160, 1440
BssMI GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
BssNAI GTATAC 1 cut(s) 184
BssT1I CCWWGG 1 cut(s) 76
Bst1107I GTATAC 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 1093
Bst4CI ACNGT 3 cut(s) 1327, 1413, 1676
Bst6I CTCTTC 2 cut(s) 273, 499
BstAPI GCANNNNNTGC 2 cut(s) 1018, 1385
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 5 cut(s) 9, 352, 374, 1474, 1642
BstDEI CTNAG 2 cut(s) 446, 1457
BstDSI CCRYGG 1 cut(s) 1160
BstF5I GGATG 6 cut(s) 26, 72, 287, 641, 1101, 1155
BstHHI GCGC 2 cut(s) 367, 1281
BstKTI GATC 6 cut(s) 437, 478, 877, 1134, 1307, 1368
BstMAI GTCTC 5 cut(s) 80, 913, 1106, 1202, 1607
BstMBI GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 8 cut(s) 139, 236, 373, 470, 629, 1018, 1379, 1385
BstNI CCWGG 1 cut(s) 1093
BstNSI RCATGY 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 1091, 1439
BstV1I GCAGC 4 cut(s) 727, 847, 1024, 1391
BstV2I GAAGAC 1 cut(s) 1441
BstX2I RGATCY 3 cut(s) 434, 874, 1304
BstXI CCANNNNNNTGG 2 cut(s) 115, 1092
BstYI RGATCY 3 cut(s) 434, 874, 1304
BstZ17I GTATAC 1 cut(s) 184
BstZI CGGCCG 1 cut(s) 670
BsuRI GGCC 3 cut(s) 444, 672, 1417
BtgI CCRYGG 1 cut(s) 1160
BtsCI GGATG 6 cut(s) 26, 72, 287, 641, 1101, 1155
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 2 cut(s) 934, 1418
Cac8I GCNNGC 5 cut(s) 9, 352, 374, 1474, 1642
CciI TCATGA 1 cut(s) 1669
CfoI GCGC 2 cut(s) 367, 1281
Cfr13I GGNCC 1 cut(s) 1032
CseI GACGC 1 cut(s) 1489
Csp6I GTAC 4 cut(s) 259, 538, 682, 866
CviQI GTAC 4 cut(s) 259, 538, 682, 866
DdeI CTNAG 2 cut(s) 446, 1457
DpnI GATC 6 cut(s) 436, 477, 876, 1133, 1306, 1367
DpnII GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
EaeI YGGCCR 2 cut(s) 670, 1415
EagI CGGCCG 1 cut(s) 670
Eam1104I CTCTTC 2 cut(s) 273, 499
EarI CTCTTC 2 cut(s) 273, 499
EclXI CGGCCG 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 444
Eco31I GGTCTC 3 cut(s) 80, 1106, 1607
Eco47I GGWCC 1 cut(s) 1032
Eco52I CGGCCG 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 753
Eco88I CYCGRG 1 cut(s) 1604
EcoO109I RGGNCCY 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 1091
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 3 cut(s) 21, 388, 1151
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 1202
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 4 cut(s) 432, 731, 763, 1045
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 2 cut(s) 183, 585
Fnu4HI GCNGC 4 cut(s) 741, 861, 1013, 1380
FokI GGATG 6 cut(s) 13, 59, 274, 648, 1108, 1142
Fsp4HI GCNGC 4 cut(s) 741, 861, 1013, 1380
FspBI CTAG 4 cut(s) 515, 1518, 1680, 1693
GlaI GCGC 2 cut(s) 366, 1280
GluI GCNGC 4 cut(s) 741, 861, 1013, 1380
GsaI CCCAGC 2 cut(s) 610, 1547
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 3 cut(s) 444, 672, 1417
HapII CCGG 2 cut(s) 576, 1440
HgaI GACGC 1 cut(s) 1489
HhaI GCGC 2 cut(s) 367, 1281
Hin6I GCGC 2 cut(s) 365, 1279
HinP1I GCGC 2 cut(s) 365, 1279
HinfI GANTC 8 cut(s) 420, 948, 1165, 1187, 1215, 1263, 1313, 1683
HpaII CCGG 2 cut(s) 576, 1440
HphI GGTGA 4 cut(s) 541, 1258, 1308, 1474
Hpy166II GTNNAC 4 cut(s) 184, 329, 549, 586
Hpy188I TCNGA 5 cut(s) 50, 68, 449, 573, 1170
Hpy188III TCNNGA 7 cut(s) 438, 851, 1191, 1507, 1612, 1670, 1680
Hpy8I GTNNAC 4 cut(s) 184, 329, 549, 586
Hpy99I CGWCG 1 cut(s) 1505
HpyAV CCTTC 2 cut(s) 493, 804
HpyCH4III ACNGT 3 cut(s) 1327, 1413, 1676
HpyCH4IV ACGT 1 cut(s) 957
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 236, 373, 470, 629, 1018, 1379, 1385
HpyF3I CTNAG 2 cut(s) 446, 1457
HpySE526I ACGT 1 cut(s) 957
HspAI GCGC 2 cut(s) 365, 1279
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 4 cut(s) 4, 470, 796, 814
Lsp1109I GCAGC 4 cut(s) 727, 847, 1024, 1391
LweI GCATC 3 cut(s) 296, 626, 778
MaeI CTAG 4 cut(s) 515, 1518, 1680, 1693
MaeII ACGT 1 cut(s) 957
MaeIII GTNAC 3 cut(s) 969, 1314, 1513
MalI GATC 6 cut(s) 436, 477, 876, 1133, 1306, 1367
MboI GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
MboII GAAGA 5 cut(s) 15, 260, 516, 1206, 1441
MfeI CAATTG 2 cut(s) 529, 798
MflI RGATCY 3 cut(s) 434, 874, 1304
MhlI GDGCHC 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 1417
MluNI TGGCCA 1 cut(s) 1417
MlyI GAGTC 4 cut(s) 414, 942, 1257, 1322
MmeI TCCRAC 3 cut(s) 28, 792, 896
Mox20I TGGCCA 1 cut(s) 1417
Mph1103I ATGCAT 3 cut(s) 21, 388, 1151
MscI TGGCCA 1 cut(s) 1417
MseI TTAA 4 cut(s) 221, 317, 693, 1391
MslI CAYNNNNRTG 6 cut(s) 299, 1007, 1109, 1232, 1253, 1271
Msp20I TGGCCA 1 cut(s) 1417
MspA1I CMGCKG 2 cut(s) 606, 632
MspI CCGG 2 cut(s) 576, 1440
MspR9I CCNGG 2 cut(s) 1093, 1441
MunI CAATTG 2 cut(s) 529, 798
Mva1269I GAATGC 3 cut(s) 19, 1014, 1151
MvaI CCWGG 1 cut(s) 1093
MwoI GCNNNNNNNGC 8 cut(s) 139, 236, 373, 470, 629, 1018, 1379, 1385
NciI CCSGG 1 cut(s) 1441
NdeII GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
NlaIV GGNNCC 2 cut(s) 229, 1033
NmeAIII GCCGAG 1 cut(s) 648
NmuCI GTSAC 2 cut(s) 1314, 1513
NsiI ATGCAT 3 cut(s) 21, 388, 1151
NspI RCATGY 1 cut(s) 11
PaeI GCATGC 1 cut(s) 11
PagI TCATGA 1 cut(s) 1669
PceI AGGCCT 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 499
PctI GAATGC 3 cut(s) 19, 1014, 1151
PfeI GAWTC 4 cut(s) 1165, 1187, 1215, 1683
PflMI CCANNNNNTGG 2 cut(s) 387, 1028
PkrI GCNGC 4 cut(s) 742, 862, 1014, 1381
PleI GAGTC 4 cut(s) 414, 942, 1257, 1321
PpsI GAGTC 4 cut(s) 414, 942, 1257, 1321
PpuMI RGGWCCY 1 cut(s) 1032
Psp5II RGGWCCY 1 cut(s) 1032
Psp6I CCWGG 1 cut(s) 1091
PspFI CCCAGC 2 cut(s) 606, 1543
PspGI CCWGG 1 cut(s) 1091
PspN4I GGNNCC 2 cut(s) 229, 1033
PspPI GGNCC 1 cut(s) 1032
PspPPI RGGWCCY 1 cut(s) 1032
PsrI GAACNNNNNNTAC 2 cut(s) 530, 562
PsuI RGATCY 3 cut(s) 434, 874, 1304
PvuII CAGCTG 2 cut(s) 606, 632
RsaI GTAC 4 cut(s) 260, 539, 683, 867
RsaNI GTAC 4 cut(s) 259, 538, 682, 866
RseI CAYNNNNRTG 6 cut(s) 299, 1007, 1109, 1232, 1253, 1271
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 4 cut(s) 221, 317, 693, 1391
SatI GCNGC 4 cut(s) 741, 861, 1013, 1380
Sau3AI GATC 6 cut(s) 434, 475, 874, 1131, 1304, 1365
Sau96I GGNCC 1 cut(s) 1032
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 4 cut(s) 414, 942, 1257, 1322
ScrFI CCNGG 2 cut(s) 1093, 1441
SduI GDGCHC 1 cut(s) 511
SfaNI GCATC 3 cut(s) 296, 626, 778
SinI GGWCC 1 cut(s) 1032
SmiMI CAYNNNNRTG 6 cut(s) 299, 1007, 1109, 1232, 1253, 1271
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
SpeI ACTAGT 1 cut(s) 1517
SphI GCATGC 1 cut(s) 11
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 2 cut(s) 912, 1445
SspMI CTAG 4 cut(s) 515, 1518, 1680, 1693
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 1091, 1439
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 1327, 1413, 1676
TaiI ACGT 1 cut(s) 960
TaqI TCGA 4 cut(s) 483, 843, 1311, 1368
TatI WGTACW 3 cut(s) 258, 681, 865
TfiI GAWTC 4 cut(s) 1165, 1187, 1215, 1683
Tru1I TTAA 4 cut(s) 221, 317, 693, 1391
Tru9I TTAA 4 cut(s) 221, 317, 693, 1391
TscAI CASTG 2 cut(s) 934, 1418
TseFI GTSAC 2 cut(s) 1314, 1513
TseI GCWGC 4 cut(s) 740, 860, 1012, 1379
Tsp45I GTSAC 2 cut(s) 1314, 1513
TspDTI ATGAA 7 cut(s) 15, 192, 897, 991, 1200, 1289, 1624
TspRI CASTG 2 cut(s) 934, 1418
Van91I CCANNNNNTGG 2 cut(s) 387, 1028
VpaK11BI GGWCC 1 cut(s) 1032
XbaI TCTAGA 1 cut(s) 1679
XceI RCATGY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 4 cut(s) 51, 115, 154, 1390
XmiI GTMKAC 2 cut(s) 183, 585
XspI CTAG 4 cut(s) 515, 1518, 1680, 1693
ZrmI AGTACT 1 cut(s) 260
Zsp2I ATGCAT 3 cut(s) 21, 388, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.