Rh7CG035800

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003229
Physical Location & Seq
Reverse (-)
2440486 .. 2444065
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG035800.1

Sequence Viewer

Length: 1680 bp
ATGAGCTTGATAGCTAACATGACTGTGTACCTTACCACAAAGTACAATCTGAGTGGTATATTTATGGTCAATGTGGTTAACATATGGAATGGTTCCTCCAACGTTGCTTCACTAGCCGGTGCTTTCGTTTCTGATACTTATCTGGGCAGATTTCGTACTCTTCTCTTTGGCTGCTTCTCATCTCTTCTGGGTATGGGGACCATAGCCCTAACTGCAAGCATACACCAATTAAGACCCTCCCACGGCTGCGACGGTCAATCTCAAGATTGCCCTCAGCCCAACAGTTGGCAGCTGGCCATACTCTTCATGGGCCTGGGCTTACTATCAATTGGAGCGGGTGGGATTAGGCCCTGCAACATTGCATTTGGTGCTGACCAATTTGACACCAGAACAGCCAAAGGAAGAGCACAACTAGAGAGCTTCTTCAATTGGTGGTACTTCTCCTTCACCGTTGCTCTTGTTATAGCTCTCACCGGAGTTGTGTATATTCAGACCAACATCAGCTGGGCCGTAGGGTTTGCTATCCCCACCGCCTGTCTTGCCTTCTCCATCACAATCTTCTTGCTTGGCCAACACGCCTACATTTACATGAAGCCCCAGGGAAGCATTTTCTCAGACGTAGCAAAAGTGATCATAGCTGCTTGTAGGAAGTCTAGGGTCAGGGTTACACCGGATTCGGAGCCCAACTTTTATGATCCTCCTTTGGTTGATAATGGGTGTGAGAGACAAGTGGTGATCAAGTACCCTCACACTAATAGGTTCAAATTCCTTGACAGAGCTGCCATCATAACAAACCCTAATCAAGAATTGGACGATCTGTGTAAGCCTAAAAGTGGGTGGAGACTATGTAGCCTTCAACAAGTTGAGCAGTTGAAGTTTCTGGTAGCGATCTTACCGGTTTGGGTAACAGCAATTGGTACTTTCATAACCATGGACCAAATGAACACTTTTGGAGTGCTACAAGCTTTGCAAATGGACAGATCAATAGGACCCAAATTTCATTTCCCACCAGGTTGGATGAACATAACCTCCATGCTCGCACTTGCTACATGGATCTTCACTTATGAGAGGATTTACCTCCCTCTAGCCAAAAAATTCAGCAGATCATCGTCAACCGATCTCCGATTCACAATGCAGCAGAGAATCAATACAGGCATCGTCCTTTCGATTTTGACAATGTTGTTGTCTGGGATCGTAGAGGAACACAGAAGGAAATCAGCATTGAAGCATGGCTCGTACATTTCGCCCACAAGTTTCGCATTGCTGTTGCCACAGTATTTCTTGACGGGCCTGACCGAGTCTTTTTCTGCTGTTGCCATAATGGAGTTCTTCACCATGCAAATGCCGGAGAGCATGAGAACTGTTGCTGGGGCAGTCTTCTTCCTTAGCTTATCTGTGTCGAATTACTTAGGCTCATTCATAGTGAACATGGTCCACAAGGCGACTGAGGGTAAATCGCAGTGGGTAGGTGGTCACGACCTTAACAAGAACAAGCTCGACTGCTACTACTACATCATTGCTGGTTTGGGAGCTCTGAATCTCGTATACTTCAATTTGTTTGCAAGACATTATGTGTTGGTCGGTAAAAAGCCTGGTCATGATAGTGGGCAAGGAGAAGTGCAGCTAGAGAATCCGAATCCAATTGCTCGTGATAGTTCAAACGACGAGAACAAAGTTTAA

Protein Analysis

559

Amino Acids

62.16

Weight (kDa)

9.02

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 58 - 489 3.8e-71 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 1545
AciI CCGC 2 cut(s) 335, 531
AclI AACGTT 1 cut(s) 102
AclWI GGATC 3 cut(s) 689, 1061, 1199
AcoI YGGCCR 2 cut(s) 294, 568
AcsI RAATTY 3 cut(s) 764, 995, 1094
AfaI GTAC 7 cut(s) 29, 44, 157, 437, 743, 919, 1238
AfiI CCNNNNNNNGG 3 cut(s) 242, 285, 833
AgeI ACCGGT 1 cut(s) 895
AgsI TTSAA 7 cut(s) 427, 763, 857, 874, 1225, 1552, 1659
AjnI CCWGG 4 cut(s) 312, 597, 1009, 1591
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
AloI GAACNNNNNNTCC 2 cut(s) 1013, 1045
Alw21I GWGCWC 2 cut(s) 409, 1534
Alw26I GTCTC 2 cut(s) 718, 835
AlwI GGATC 3 cut(s) 689, 1061, 1199
AoxI GGCC 6 cut(s) 294, 310, 347, 507, 568, 1288
ApeKI GCWGC 7 cut(s) 171, 246, 289, 638, 779, 1135, 1621
ApoI RAATTY 3 cut(s) 764, 995, 1094
AsiGI ACCGGT 1 cut(s) 895
AspS9I GGNCC 8 cut(s) 198, 310, 348, 507, 934, 989, 1288, 1432
AsuHPI GGTGA 4 cut(s) 439, 463, 745, 1324
AvaII GGWCC 4 cut(s) 198, 934, 989, 1432
BalI TGGCCA 2 cut(s) 296, 570
BanII GRGCYC 2 cut(s) 684, 1534
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
BauI CACGAG 1 cut(s) 1647
BbsI GAAGAC 1 cut(s) 1369
Bbv12I GWGCWC 2 cut(s) 409, 1534
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 7 cut(s) 158, 233, 301, 625, 766, 1147, 1633
BccI CCATC 2 cut(s) 557, 791
BceAI ACGGC 2 cut(s) 259, 494
BciT130I CCWGG 4 cut(s) 314, 599, 1011, 1593
BclI TGATCA 2 cut(s) 630, 735
BcoDI GTCTC 2 cut(s) 718, 835
BfaI CTAG 5 cut(s) 113, 413, 654, 1085, 1625
BisI GCNGC 7 cut(s) 172, 247, 290, 639, 780, 1136, 1622
BlsI GCNGC 7 cut(s) 173, 248, 291, 640, 781, 1137, 1623
Bme1390I CCNGG 4 cut(s) 314, 599, 1011, 1593
Bme18I GGWCC 4 cut(s) 198, 934, 989, 1432
BmgT120I GGNCC 8 cut(s) 198, 310, 348, 507, 934, 989, 1288, 1432
BmiI GGNNCC 4 cut(s) 94, 199, 681, 991
BmrFI CCNGG 4 cut(s) 314, 599, 1011, 1593
BmsI GCATC 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 1369
Bpu10I CCTNAGC 2 cut(s) 273, 1385
BpuEI CTTGAG 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 5 cut(s) 241, 313, 597, 598, 930
BsaWI WCCGGW 3 cut(s) 473, 670, 895
BsaXI ACNNNNNCTCC 4 cut(s) 832, 862, 1013, 1043
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 285, 833
Bse118I RCCGGY 2 cut(s) 116, 895
Bse3DI GCAATG 3 cut(s) 357, 1259, 1515
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 4 cut(s) 314, 599, 1011, 1593
BseDI CCNNGG 5 cut(s) 241, 313, 597, 598, 930
BseGI GGATG 1 cut(s) 1023
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 3 cut(s) 242, 285, 833
BseMI GCAATG 3 cut(s) 357, 1259, 1515
BseMII CTCAG 4 cut(s) 41, 287, 627, 1437
BseXI GCAGC 7 cut(s) 158, 233, 301, 625, 766, 1147, 1633
BseYI CCCAGC 2 cut(s) 504, 1367
BsgI GTGCAG 1 cut(s) 1640
BshFI GGCC 6 cut(s) 296, 312, 349, 509, 570, 1290
BshTI ACCGGT 1 cut(s) 895
BsiHKAI GWGCWC 2 cut(s) 409, 1534
BsiSI CCGG 5 cut(s) 117, 474, 671, 896, 1346
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 3 cut(s) 242, 285, 833
BsmAI GTCTC 2 cut(s) 718, 835
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 6 cut(s) 296, 312, 349, 509, 570, 1290
Bsp1286I GDGCHC 3 cut(s) 409, 684, 1534
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 2 cut(s) 335, 531
BspANI GGCC 6 cut(s) 296, 312, 349, 509, 570, 1290
BspCNI CTCAG 4 cut(s) 42, 286, 626, 1438
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 4 cut(s) 94, 199, 681, 991
BspPI GGATC 3 cut(s) 689, 1061, 1199
BspQI GCTCTTC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 335
BsrDI GCAATG 3 cut(s) 357, 1259, 1515
BsrFI RCCGGY 2 cut(s) 116, 895
BssAI RCCGGY 2 cut(s) 116, 895
BssECI CCNNGG 5 cut(s) 241, 313, 597, 598, 930
BssNAI GTATAC 1 cut(s) 1546
BssSI CACGAG 1 cut(s) 1647
BssT1I CCWWGG 1 cut(s) 930
Bst1107I GTATAC 1 cut(s) 1546
Bst2BI CACGAG 1 cut(s) 1647
Bst2UI CCWGG 4 cut(s) 314, 599, 1011, 1593
Bst4CI ACNGT 6 cut(s) 25, 254, 284, 451, 1275, 1363
Bst6I CTCTTC 4 cut(s) 165, 189, 308, 397
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 217, 294, 1038
BstDEI CTNAG 6 cut(s) 50, 273, 613, 1385, 1408, 1446
BstDSI CCRYGG 2 cut(s) 241, 930
BstF5I GGATG 1 cut(s) 1023
BstMAI GTCTC 2 cut(s) 718, 835
BstMWI GCNNNNNNNGC 4 cut(s) 113, 212, 368, 539
BstNI CCWGG 4 cut(s) 314, 599, 1011, 1593
BstSCI CCNGG 4 cut(s) 312, 597, 1009, 1591
BstV1I GCAGC 7 cut(s) 158, 233, 301, 625, 766, 1147, 1633
BstV2I GAAGAC 1 cut(s) 1369
BstX2I RGATCY 1 cut(s) 1053
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 1 cut(s) 1053
BstZ17I GTATAC 1 cut(s) 1546
BsuRI GGCC 6 cut(s) 296, 312, 349, 509, 570, 1290
BtgI CCRYGG 2 cut(s) 241, 930
BtsCI GGATG 1 cut(s) 1023
BtsI GCAGTG 1 cut(s) 1466
BtsIMutI CAGTG 1 cut(s) 1466
Cac8I GCNNGC 3 cut(s) 217, 294, 1038
CciI TCATGA 1 cut(s) 1597
Cfr10I RCCGGY 2 cut(s) 116, 895
Cfr13I GGNCC 8 cut(s) 198, 310, 348, 507, 934, 989, 1288, 1432
CsiI ACCWGGT 1 cut(s) 1009
Csp6I GTAC 7 cut(s) 28, 43, 156, 436, 742, 918, 1237
CspAI ACCGGT 1 cut(s) 895
CspCI CAANNNNNGTGG 2 cut(s) 34, 69
CviQI GTAC 7 cut(s) 28, 43, 156, 436, 742, 918, 1237
DdeI CTNAG 6 cut(s) 50, 273, 613, 1385, 1408, 1446
EaeI YGGCCR 2 cut(s) 294, 568
Eam1104I CTCTTC 4 cut(s) 165, 189, 308, 397
EarI CTCTTC 4 cut(s) 165, 189, 308, 397
Ecl136II GAGCTC 1 cut(s) 1532
Eco130I CCWWGG 1 cut(s) 930
Eco24I GRGCYC 2 cut(s) 684, 1534
Eco47I GGWCC 4 cut(s) 198, 934, 989, 1432
Eco53kI GAGCTC 1 cut(s) 1532
EcoICRI GAGCTC 1 cut(s) 1532
EcoO109I RGGNCCY 2 cut(s) 348, 989
EcoRII CCWGG 4 cut(s) 312, 597, 1009, 1591
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 2 cut(s) 684, 1534
ErhI CCWWGG 1 cut(s) 930
FaqI GGGAC 1 cut(s) 211
FauI CCCGC 1 cut(s) 328
FauNDI CATATG 1 cut(s) 83
FbaI TGATCA 2 cut(s) 630, 735
FblI GTMKAC 1 cut(s) 1545
Fnu4HI GCNGC 7 cut(s) 172, 247, 290, 639, 780, 1136, 1622
FokI GGATG 1 cut(s) 1030
FriOI GRGCYC 2 cut(s) 684, 1534
Fsp4HI GCNGC 7 cut(s) 172, 247, 290, 639, 780, 1136, 1622
FspBI CTAG 5 cut(s) 113, 413, 654, 1085, 1625
GluI GCNGC 7 cut(s) 172, 247, 290, 639, 780, 1136, 1622
GsaI CCCAGC 2 cut(s) 508, 1371
HaeIII GGCC 6 cut(s) 296, 312, 349, 509, 570, 1290
HapII CCGG 5 cut(s) 117, 474, 671, 896, 1346
HincII GTYRAC 2 cut(s) 79, 1113
HindII GTYRAC 2 cut(s) 79, 1113
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 7 cut(s) 674, 1125, 1143, 1298, 1537, 1630, 1636
HpaI GTTAAC 1 cut(s) 79
HpaII CCGG 5 cut(s) 117, 474, 671, 896, 1346
HphI GGTGA 4 cut(s) 439, 463, 745, 1324
Hpy166II GTNNAC 6 cut(s) 28, 79, 1113, 1426, 1435, 1546
Hpy188I TCNGA 8 cut(s) 51, 133, 492, 616, 679, 1124, 1536, 1635
Hpy188III TCNNGA 6 cut(s) 263, 803, 1282, 1475, 1598, 1649
Hpy8I GTNNAC 6 cut(s) 28, 79, 1113, 1426, 1435, 1546
Hpy99I CGWCG 2 cut(s) 254, 1667
HpyAV CCTTC 4 cut(s) 454, 553, 863, 1203
HpyCH4III ACNGT 6 cut(s) 25, 254, 284, 451, 1275, 1363
HpyCH4IV ACGT 2 cut(s) 102, 618
HpyCH4V TGCA 8 cut(s) 215, 354, 362, 970, 1135, 1339, 1562, 1621
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 212, 368, 539
HpyF3I CTNAG 6 cut(s) 50, 273, 613, 1385, 1408, 1446
HpySE526I ACGT 2 cut(s) 102, 618
Ksp22I TGATCA 2 cut(s) 630, 735
KspAI GTTAAC 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 397
LmnI GCTCC 3 cut(s) 332, 679, 1529
Lsp1109I GCAGC 7 cut(s) 158, 233, 301, 625, 766, 1147, 1633
LweI GCATC 1 cut(s) 1164
MabI ACCWGGT 1 cut(s) 1009
MaeI CTAG 5 cut(s) 113, 413, 654, 1085, 1625
MaeII ACGT 2 cut(s) 102, 618
MaeIII GTNAC 3 cut(s) 664, 904, 1472
MbiI CCGCTC 1 cut(s) 335
MfeI CAATTG 4 cut(s) 327, 427, 912, 1641
MflI RGATCY 1 cut(s) 1053
MhlI GDGCHC 3 cut(s) 409, 684, 1534
MlsI TGGCCA 2 cut(s) 296, 570
MluNI TGGCCA 2 cut(s) 296, 570
MlyI GAGTC 1 cut(s) 1307
MmeI TCCRAC 2 cut(s) 123, 995
Mox20I TGGCCA 2 cut(s) 296, 570
MscI TGGCCA 2 cut(s) 296, 570
MseI TTAA 4 cut(s) 78, 230, 1482, 1678
MslI CAYNNNNRTG 5 cut(s) 23, 587, 929, 1340, 1602
Msp20I TGGCCA 2 cut(s) 296, 570
MspA1I CMGCKG 2 cut(s) 292, 504
MspI CCGG 5 cut(s) 117, 474, 671, 896, 1346
MspR9I CCNGG 4 cut(s) 314, 599, 1011, 1593
MunI CAATTG 4 cut(s) 327, 427, 912, 1641
MvaI CCWGG 4 cut(s) 314, 599, 1011, 1593
MwoI GCNNNNNNNGC 4 cut(s) 113, 212, 368, 539
NcoI CCATGG 1 cut(s) 930
NdeI CATATG 1 cut(s) 83
NlaIV GGNNCC 4 cut(s) 94, 199, 681, 991
NmuCI GTSAC 1 cut(s) 1472
PagI TCATGA 1 cut(s) 1597
PasI CCCWGGG 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 397
PfeI GAWTC 6 cut(s) 674, 1125, 1143, 1537, 1630, 1636
PflFI GACNNNGTC 1 cut(s) 1297
PflMI CCANNNNNTGG 1 cut(s) 285
PinAI ACCGGT 1 cut(s) 895
PkrI GCNGC 7 cut(s) 173, 248, 291, 640, 781, 1137, 1623
PleI GAGTC 1 cut(s) 1306
PpsI GAGTC 1 cut(s) 1306
PpuMI RGGWCCY 1 cut(s) 989
Psp124BI GAGCTC 1 cut(s) 1534
Psp1406I AACGTT 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 989
Psp6I CCWGG 4 cut(s) 312, 597, 1009, 1591
PspFI CCCAGC 2 cut(s) 504, 1367
PspGI CCWGG 4 cut(s) 312, 597, 1009, 1591
PspN4I GGNNCC 4 cut(s) 94, 199, 681, 991
PspPI GGNCC 8 cut(s) 198, 310, 348, 507, 934, 989, 1288, 1432
PspPPI RGGWCCY 1 cut(s) 989
PsuI RGATCY 1 cut(s) 1053
PsyI GACNNNGTC 1 cut(s) 1297
PvuII CAGCTG 2 cut(s) 292, 504
RsaI GTAC 7 cut(s) 29, 44, 157, 437, 743, 919, 1238
RsaNI GTAC 7 cut(s) 28, 43, 156, 436, 742, 918, 1237
RseI CAYNNNNRTG 5 cut(s) 23, 587, 929, 1340, 1602
SacI GAGCTC 1 cut(s) 1534
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 4 cut(s) 78, 230, 1482, 1678
SatI GCNGC 7 cut(s) 172, 247, 290, 639, 780, 1136, 1622
Sau96I GGNCC 8 cut(s) 198, 310, 348, 507, 934, 989, 1288, 1432
SchI GAGTC 1 cut(s) 1307
ScrFI CCNGG 4 cut(s) 314, 599, 1011, 1593
SduI GDGCHC 3 cut(s) 409, 684, 1534
SexAI ACCWGGT 1 cut(s) 1009
SfaNI GCATC 1 cut(s) 1164
SinI GGWCC 4 cut(s) 198, 934, 989, 1432
SmiMI CAYNNNNRTG 5 cut(s) 23, 587, 929, 1340, 1602
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
SsiI CCGC 2 cut(s) 335, 531
SspMI CTAG 5 cut(s) 113, 413, 654, 1085, 1625
SstI GAGCTC 1 cut(s) 1534
StyD4I CCNGG 4 cut(s) 312, 597, 1009, 1591
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 6 cut(s) 25, 254, 284, 451, 1275, 1363
TaiI ACGT 2 cut(s) 105, 621
TaqI TCGA 3 cut(s) 1166, 1400, 1497
TaqII GACCGA 1 cut(s) 1310
TatI WGTACW 1 cut(s) 42
TfiI GAWTC 6 cut(s) 674, 1125, 1143, 1537, 1630, 1636
Tru1I TTAA 4 cut(s) 78, 230, 1482, 1678
Tru9I TTAA 4 cut(s) 78, 230, 1482, 1678
TscAI CASTG 1 cut(s) 1466
TseFI GTSAC 1 cut(s) 1472
TseI GCWGC 7 cut(s) 171, 246, 289, 638, 779, 1135, 1621
Tsp45I GTSAC 1 cut(s) 1472
TspDTI ATGAA 7 cut(s) 295, 605, 913, 956, 989, 1034, 1408
TspRI CASTG 1 cut(s) 1466
Tth111I GACNNNGTC 1 cut(s) 1297
Van91I CCANNNNNTGG 1 cut(s) 285
VpaK11BI GGWCC 4 cut(s) 198, 934, 989, 1432
XapI RAATTY 3 cut(s) 764, 995, 1094
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 1545
XspI CTAG 5 cut(s) 113, 413, 654, 1085, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.