MD04G1137500.v1.1

Protein NRT1 PTR FAMILY 2.8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003229
Physical Location & Seq
Forward (+)
22509802 .. 22511767
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1137500.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGAGCATGCAGCGCATTCTCCATCTGAAGGGCTAGAGAGTCAATCCCTTCCTCAGAAAAGACCGGTTGGAGGATGGAGAGCCGTCAGATACATTCTCGGGAATGAGACGTTCGAGAAGTTGGCTTCCATGAGTTTGATTGCGAATCTGGTGGTGTATCTGCACACAAAGTACAACTTGGACAATGTGGTTTCAGCTAATGTGTTCAATATATGGTCTGGTTCATGCAACATTGCACCACTGTTTGGTGCTTTTCTTGCTGATAGGTATTTGGGAAAGTTTAATACTCTTCTTTTCAGCTCGATAGCATCACTTCTGGGAATGGGGACCCTGACCTTAACCGCAGGCATACATAAATTAACTCCGTCTGCCTGCATCGCACAAACTGCAGAATGCCAACAGCCGAGTACTTGGCAGATAGCGATCCTGTATTTGGGTCTCGCGTTGCTTGTTGTTGGATCCGGAGGCCTTAGACCATGCAACATTGCCTTTGGAGCTGATCAGTTTGACACCACAACGGAGAAGGGAAGAGCGCAGCTCGATAGCTTTTGCAATTTGTGGTACCTCTTGTTCACTCTAGCCCTCCTCATCGCTCTCACCATCGTGGTTTACATTCAGACGAATGTCAGCTGGATTTTGGGTTTTGCTGTCCCAACTGGCTGCTTTGCTCTCTCCATTATAATTTTCTTGCTGGGAACAAGGTTGTACGTGCGCGTAAAGCCCCAAGGAAGCATCTTTGCCGACATTATCAAGGTTCTAGTTGCCACCTGCAGAAAATGTGGTTCGAAATCTAATGCTGGAGAAACTTCAGGGCAGTCATACTATGATCCTCTGGTTGGATCAGAGTCAGAGAGAGAAAGAGCAGCGCGTACCAACAAACTCAAGTTCTTCGATAAGGCTGCTATGATCGTAGACCCGAGCGAATTGGACAGCCAAGGGAAGCCCAAAAACAGTTGGAAATTATGCAGTGTGCAACAAGTGGAGCAACTAAAATCTGTGGTGAGGATTTTGCCTGTTTGGATAACTGGAATTGTTTGTTTCGTAGGCATGCAGCAAATGAATTCATTCGGGATCTTTCAAGCGATTCAAATGAACAAATCGATTGGCCCCAAATTCCAGATTCCACCAGCCTGGATGGGCCTAACACCGATGATTGCACTTTCGATATGGATCATCATCTACGAGAGCATCTACGTTCCTCAAATGCAGAAACGGAGCAAGAACGAAACTGGAAGATTGACAATGGAGCAGAGGTTTAAGATTGGTATTGTGATCTCAGTTCTGTGCATGGTAGTGGCTGGACTTACAGAAATGAAGCGCCGCGACTCAGCTTTGGCACACGGGACCCTCGAGTCACCAATAACCGTTGCCTTGCTCGTGCCGCAGTTCGCCTTATCGGGTCTGATTGAAGCCTTTGCTGCAATTGCCTTGATGGAGCTGCTCACCACTCAATGGCCTCAGAGCATGAGGACTTTCGCCGGGGCGGTTTTCTTCCTCAGCTTGTCCATTGCAAGCTACCTAACCACCATTCTCATCAACGTCGTCAAAAAATTGAGCGGACTGAACGGAAATTCATCATGGTTGGGAGGTAATGACCTCAACAAAAACAGACTTGATTACTACTATTACACGATAGCCGCCTTCGGGGTCTTAAATTTCGTGTATTTCCATTTCTTCGCTCGCCATTATCTGTCCGACGACGTTGCTCAACACTCCGAAAAGCCGTCCGACTGTGACTCCTCAGGCAATGGAAAAGGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04974 map04974
Pfam Domains
Protein Families
PTR2

Protein Analysis

588

Amino Acids

64.55

Weight (kDa)

8.98

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 94 - 528 1.1e-76 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
AarI CACCTGC 1 cut(s) 776
AasI GACNNNNNNGTC 1 cut(s) 1351
Acc36I ACCTGC 1 cut(s) 776
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 561
AccB7I CCANNNNNTGG 2 cut(s) 245, 1452
AccBSI CCGCTC 1 cut(s) 1557
AccI GTMKAC 1 cut(s) 912
AccII CGCG 4 cut(s) 443, 714, 868, 1323
AccIII TCCGGA 1 cut(s) 461
AciI CCGC 6 cut(s) 342, 1321, 1382, 1484, 1557, 1638
AclWI GGATC 7 cut(s) 418, 453, 466, 821, 847, 1079, 1178
AcsI RAATTY 4 cut(s) 1060, 1112, 1570, 1654
AcuI CTGAAG 2 cut(s) 48, 792
AfaI GTAC 5 cut(s) 173, 409, 563, 707, 871
AfiI CCNNNNNNNGG 7 cut(s) 29, 71, 245, 433, 836, 1452, 1644
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 208, 1079, 1088, 1409
AjnI CCWGG 1 cut(s) 1130
AleI CACNNNNGTG 1 cut(s) 602
Alw26I GTCTC 2 cut(s) 101, 443
AlwI GGATC 7 cut(s) 418, 453, 466, 821, 847, 1079, 1178
Ama87I CYCGRG 3 cut(s) 98, 916, 1349
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 4 cut(s) 466, 1105, 1138, 1454
ApeKI GCWGC 8 cut(s) 11, 535, 660, 863, 899, 1051, 1418, 1438
ApoI RAATTY 4 cut(s) 1060, 1112, 1570, 1654
AsiGI ACCGGT 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 1064
Asp718I GGTACC 1 cut(s) 561
AspLEI GCGC 5 cut(s) 16, 535, 714, 868, 1320
AspS9I GGNCC 4 cut(s) 327, 1106, 1138, 1344
AsuC2I CCSGG 1 cut(s) 1480
AsuHPI GGTGA 4 cut(s) 589, 1012, 1347, 1435
AsuII TTCGAA 1 cut(s) 785
AvaI CYCGRG 3 cut(s) 98, 916, 1349
AvaII GGWCC 2 cut(s) 327, 1344
AxyI CCTNAGG 1 cut(s) 1741
BamHI GGATCC 1 cut(s) 458
BanI GGYRCC 1 cut(s) 561
BauI CACGAG 1 cut(s) 1376
BbvCI CCTCAGC 1 cut(s) 1496
BbvI GCAGC 8 cut(s) 23, 547, 647, 875, 886, 1063, 1405, 1425
BccI CCATC 5 cut(s) 31, 69, 608, 1129, 1426
BceAI ACGGC 2 cut(s) 68, 1708
BcgI CGANNNNNNTGC 4 cut(s) 881, 915, 1685, 1719
BciT130I CCWGG 1 cut(s) 1132
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 1 cut(s) 1480
BcoDI GTCTC 2 cut(s) 101, 443
BfaI CTAG 3 cut(s) 35, 578, 758
BfmI CTRYAG 2 cut(s) 387, 769
BfoI RGCGCY 1 cut(s) 1321
BfuAI ACCTGC 1 cut(s) 776
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 1132, 1480
Bme18I GGWCC 2 cut(s) 327, 1344
BmeT110I CYCGRG 3 cut(s) 98, 916, 1349
BmgT120I GGNCC 4 cut(s) 327, 1106, 1138, 1344
BmiI GGNNCC 7 cut(s) 328, 329, 460, 563, 1108, 1345, 1346
BmrFI CCNGG 2 cut(s) 1132, 1480
BmsI GCATC 4 cut(s) 317, 384, 741, 1197
BoxI GACNNNNGTC 1 cut(s) 623
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 1 cut(s) 819
Bpu10I CCTNAGC 1 cut(s) 1496
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 1480
Bsa29I ATCGAT 1 cut(s) 1100
BsaAI YACGTR 1 cut(s) 709
BsaBI GATNNNNATC 3 cut(s) 422, 1169, 1175
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 724, 934, 1479
BsaWI WCCGGW 2 cut(s) 64, 461
BsaXI ACNNNNNCTCC 4 cut(s) 1238, 1268, 1721, 1751
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 71, 245, 433, 836, 1452, 1644
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 3 cut(s) 661, 1030, 1234
Bse21I CCTNAGG 1 cut(s) 1741
Bse3DI GCAATG 4 cut(s) 231, 483, 1506, 1753
Bse8I GATNNNNATC 3 cut(s) 422, 1169, 1175
BseAI TCCGGA 1 cut(s) 461
BseBI CCWGG 1 cut(s) 1132
BseCI ATCGAT 1 cut(s) 1100
BseDI CCNNGG 3 cut(s) 724, 934, 1479
BseGI GGATG 2 cut(s) 80, 1140
BseJI GATNNNNATC 3 cut(s) 422, 1169, 1175
BseLI CCNNNNNNNGG 7 cut(s) 29, 71, 245, 433, 836, 1452, 1644
BseMI GCAATG 4 cut(s) 231, 483, 1506, 1753
BseMII CTCAG 6 cut(s) 68, 1290, 1341, 1472, 1510, 1755
BseNI ACTGG 3 cut(s) 661, 1030, 1234
BseRI GAGGAG 2 cut(s) 575, 1729
BseXI GCAGC 8 cut(s) 23, 547, 647, 875, 886, 1063, 1405, 1425
BseYI CCCAGC 1 cut(s) 691
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 4 cut(s) 443, 714, 868, 1323
BshFI GGCC 4 cut(s) 468, 1107, 1140, 1456
BshNI GGYRCC 1 cut(s) 561
BshTI ACCGGT 1 cut(s) 64
BshVI ATCGAT 1 cut(s) 1100
BsiHKCI CYCGRG 3 cut(s) 98, 916, 1349
BsiSI CCGG 3 cut(s) 65, 462, 1479
BslFI GGGAC 3 cut(s) 340, 635, 1357
BslI CCNNNNNNNGG 7 cut(s) 29, 71, 245, 433, 836, 1452, 1644
BsmAI GTCTC 2 cut(s) 101, 443
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 3 cut(s) 340, 635, 1357
BsmI GAATGC 2 cut(s) 16, 398
BsnI GGCC 4 cut(s) 468, 1107, 1140, 1456
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 3 cut(s) 98, 916, 1349
Bsp119I TTCGAA 1 cut(s) 785
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
BspACI CCGC 6 cut(s) 342, 1321, 1382, 1484, 1557, 1638
BspANI GGCC 4 cut(s) 468, 1107, 1140, 1456
BspCNI CTCAG 6 cut(s) 67, 1289, 1340, 1471, 1509, 1754
BspDI ATCGAT 1 cut(s) 1100
BspEI TCCGGA 1 cut(s) 461
BspFNI CGCG 4 cut(s) 443, 714, 868, 1323
BspLI GGNNCC 7 cut(s) 328, 329, 460, 563, 1108, 1345, 1346
BspMAI CTGCAG 2 cut(s) 391, 773
BspMI ACCTGC 1 cut(s) 776
BspPI GGATC 7 cut(s) 418, 453, 466, 821, 847, 1079, 1178
BspQI GCTCTTC 1 cut(s) 523
BspT104I TTCGAA 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 561
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 1557
BsrDI GCAATG 4 cut(s) 231, 483, 1506, 1753
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 3 cut(s) 661, 1030, 1234
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 724, 934, 1479
BssMI GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
BssSI CACGAG 1 cut(s) 1376
BssT1I CCWWGG 2 cut(s) 724, 934
Bst2BI CACGAG 1 cut(s) 1376
Bst2UI CCWGG 1 cut(s) 1132
Bst4CI ACNGT 4 cut(s) 243, 953, 1366, 1733
Bst6I CTCTTC 2 cut(s) 294, 523
BstAPI GCANNNNNTGC 1 cut(s) 386
BstBAI YACGTR 1 cut(s) 709
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 6 cut(s) 9, 346, 373, 1049, 1513, 1681
BstDEI CTNAG 7 cut(s) 54, 470, 1276, 1327, 1458, 1496, 1741
BstF5I GGATG 2 cut(s) 80, 1140
BstFNI CGCG 4 cut(s) 443, 714, 868, 1323
BstH2I RGCGCY 1 cut(s) 1321
BstHHI GCGC 5 cut(s) 16, 535, 714, 868, 1320
BstKTI GATC 9 cut(s) 426, 461, 502, 829, 842, 909, 1074, 1173, 1275
BstMAI GTCTC 2 cut(s) 101, 443
BstMBI GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
BstMWI GCNNNNNNNGC 9 cut(s) 13, 257, 377, 386, 494, 718, 1381, 1418, 1424
BstNI CCWGG 1 cut(s) 1132
BstNSI RCATGY 2 cut(s) 11, 1051
BstPAI GACNNNNGTC 1 cut(s) 623
BstSCI CCNGG 2 cut(s) 1130, 1478
BstSFI CTRYAG 2 cut(s) 387, 769
BstUI CGCG 4 cut(s) 443, 714, 868, 1323
BstV1I GCAGC 8 cut(s) 23, 547, 647, 875, 886, 1063, 1405, 1425
BstX2I RGATCY 2 cut(s) 458, 1071
BstXI CCANNNNNNTGG 1 cut(s) 1131
BstYI RGATCY 2 cut(s) 458, 1071
Bsu15I ATCGAT 1 cut(s) 1100
Bsu36I CCTNAGG 1 cut(s) 1741
BsuRI GGCC 4 cut(s) 468, 1107, 1140, 1456
BsuTUI ATCGAT 1 cut(s) 1100
BtgZI GCGATG 2 cut(s) 361, 574
BtsCI GGATG 2 cut(s) 80, 1140
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 2 cut(s) 239, 973
BveI ACCTGC 1 cut(s) 776
Cac8I GCNNGC 6 cut(s) 9, 346, 373, 1049, 1513, 1681
CfoI GCGC 5 cut(s) 16, 535, 714, 868, 1320
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 4 cut(s) 327, 1106, 1138, 1344
ClaI ATCGAT 1 cut(s) 1100
Csp6I GTAC 5 cut(s) 172, 408, 562, 706, 870
CspAI ACCGGT 1 cut(s) 64
CviAII CATG 8 cut(s) 8, 130, 225, 477, 1048, 1288, 1465, 1578
CviQI GTAC 5 cut(s) 172, 408, 562, 706, 870
DdeI CTNAG 7 cut(s) 54, 470, 1276, 1327, 1458, 1496, 1741
DpnI GATC 9 cut(s) 425, 460, 501, 828, 841, 908, 1073, 1172, 1274
DpnII GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
DrdI GACNNNNNNGTC 1 cut(s) 1351
DseDI GACNNNNNNGTC 1 cut(s) 1351
Eam1104I CTCTTC 2 cut(s) 294, 523
EarI CTCTTC 2 cut(s) 294, 523
Eco130I CCWWGG 2 cut(s) 724, 934
Eco147I AGGCCT 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 327, 1344
Eco57I CTGAAG 2 cut(s) 48, 792
Eco81I CCTNAGG 1 cut(s) 1741
Eco88I CYCGRG 3 cut(s) 98, 916, 1349
EcoO109I RGGNCCY 2 cut(s) 327, 1344
EcoRI GAATTC 1 cut(s) 1060
EcoRII CCWGG 1 cut(s) 1130
EcoT14I CCWWGG 2 cut(s) 724, 934
ErhI CCWWGG 2 cut(s) 724, 934
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 8 cut(s) 11, 133, 228, 480, 1051, 1291, 1468, 1581
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 3 cut(s) 340, 635, 1357
FatI CATG 8 cut(s) 7, 129, 224, 476, 1047, 1287, 1464, 1577
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 912
FokI GGATG 2 cut(s) 87, 1147
FspBI CTAG 3 cut(s) 35, 578, 758
GlaI GCGC 5 cut(s) 15, 534, 713, 867, 1319
GsaI CCCAGC 1 cut(s) 695
GsuI CTGGAG 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 1321
HaeIII GGCC 4 cut(s) 468, 1107, 1140, 1456
HapII CCGG 3 cut(s) 65, 462, 1479
HhaI GCGC 5 cut(s) 16, 535, 714, 868, 1320
Hin1II CATG 8 cut(s) 11, 133, 228, 480, 1051, 1291, 1468, 1581
Hin6I GCGC 5 cut(s) 14, 533, 712, 866, 1318
HinP1I GCGC 5 cut(s) 14, 533, 712, 866, 1318
HinfI GANTC 8 cut(s) 40, 145, 845, 1084, 1120, 1325, 1352, 1736
HpaII CCGG 3 cut(s) 65, 462, 1479
HphI GGTGA 4 cut(s) 589, 1012, 1347, 1435
Hpy166II GTNNAC 3 cut(s) 573, 610, 913
Hpy188III TCNNGA 5 cut(s) 100, 115, 462, 1069, 1117
Hpy8I GTNNAC 3 cut(s) 573, 610, 913
Hpy99I CGWCG 3 cut(s) 1544, 1700, 1703
HpyAV CCTTC 4 cut(s) 23, 59, 517, 1651
HpyCH4III ACNGT 4 cut(s) 243, 953, 1366, 1733
HpyCH4IV ACGT 5 cut(s) 110, 708, 1194, 1539, 1701
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 257, 377, 386, 494, 718, 1381, 1418, 1424
HpyF3I CTNAG 7 cut(s) 54, 470, 1276, 1327, 1458, 1496, 1741
HpySE526I ACGT 5 cut(s) 110, 708, 1194, 1539, 1701
Hsp92II CATG 8 cut(s) 11, 133, 228, 480, 1051, 1291, 1468, 1581
HspAI GCGC 5 cut(s) 14, 533, 712, 866, 1318
KflI GGGWCCC 2 cut(s) 327, 1344
Kpn2I TCCGGA 1 cut(s) 461
KpnI GGTACC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
LguI GCTCTTC 1 cut(s) 523
LmnI GCTCC 6 cut(s) 4, 494, 982, 1215, 1246, 1435
Lsp1109I GCAGC 8 cut(s) 23, 547, 647, 875, 886, 1063, 1405, 1425
LweI GCATC 4 cut(s) 317, 384, 741, 1197
MaeI CTAG 3 cut(s) 35, 578, 758
MaeII ACGT 5 cut(s) 110, 708, 1194, 1539, 1701
MaeIII GTNAC 2 cut(s) 1353, 1733
MalI GATC 9 cut(s) 425, 460, 501, 828, 841, 908, 1073, 1172, 1274
MbiI CCGCTC 1 cut(s) 1557
MboI GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
MboII GAAGA 6 cut(s) 281, 540, 880, 1245, 1483, 1666
MfeI CAATTG 1 cut(s) 1422
MflI RGATCY 2 cut(s) 458, 1071
MlyI GAGTC 5 cut(s) 49, 854, 1319, 1361, 1730
MmeI TCCRAC 6 cut(s) 49, 436, 817, 935, 1719, 1752
MroI TCCGGA 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 1064
MseI TTAA 5 cut(s) 282, 338, 359, 1257, 1652
MslI CAYNNNNRTG 2 cut(s) 602, 1292
MspA1I CMGCKG 1 cut(s) 630
MspI CCGG 3 cut(s) 65, 462, 1479
MspR9I CCNGG 2 cut(s) 1132, 1480
MunI CAATTG 1 cut(s) 1422
Mva1269I GAATGC 2 cut(s) 16, 398
MvaI CCWGG 1 cut(s) 1132
MvnI CGCG 4 cut(s) 443, 714, 868, 1323
MwoI GCNNNNNNNGC 9 cut(s) 13, 257, 377, 386, 494, 718, 1381, 1418, 1424
NciI CCSGG 1 cut(s) 1480
NdeII GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
NlaIII CATG 8 cut(s) 11, 133, 228, 480, 1051, 1291, 1468, 1581
NlaIV GGNNCC 7 cut(s) 328, 329, 460, 563, 1108, 1345, 1346
NmeAIII GCCGAG 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 1353, 1733
NspI RCATGY 2 cut(s) 11, 1051
NspV TTCGAA 1 cut(s) 785
OliI CACNNNNGTG 1 cut(s) 602
PaeI GCATGC 2 cut(s) 11, 1051
PaeR7I CTCGAG 1 cut(s) 1349
PaqCI CACCTGC 1 cut(s) 776
PceI AGGCCT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 1 cut(s) 1347
PctI GAATGC 2 cut(s) 16, 398
PdmI GAANNNNTTC 1 cut(s) 1064
PfeI GAWTC 3 cut(s) 145, 1084, 1120
PflMI CCANNNNNTGG 2 cut(s) 245, 1452
PinAI ACCGGT 1 cut(s) 64
PleI GAGTC 5 cut(s) 48, 853, 1319, 1360, 1730
PpsI GAGTC 5 cut(s) 48, 853, 1319, 1360, 1730
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 2 cut(s) 327, 1344
PshAI GACNNNNGTC 1 cut(s) 623
PsiI TTATAA 1 cut(s) 680
Psp5II RGGWCCY 2 cut(s) 327, 1344
Psp6I CCWGG 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 691
PspGI CCWGG 1 cut(s) 1130
PspN4I GGNNCC 7 cut(s) 328, 329, 460, 563, 1108, 1345, 1346
PspPI GGNCC 4 cut(s) 327, 1106, 1138, 1344
PspPPI RGGWCCY 2 cut(s) 327, 1344
PspXI VCTCGAGB 1 cut(s) 1349
PsrI GAACNNNNNNTAC 2 cut(s) 554, 586
PstI CTGCAG 2 cut(s) 391, 773
PsuI RGATCY 2 cut(s) 458, 1071
PvuII CAGCTG 1 cut(s) 630
RsaI GTAC 5 cut(s) 173, 409, 563, 707, 871
RsaNI GTAC 5 cut(s) 172, 408, 562, 706, 870
RseI CAYNNNNRTG 2 cut(s) 602, 1292
SapI GCTCTTC 1 cut(s) 523
SaqAI TTAA 5 cut(s) 282, 338, 359, 1257, 1652
Sau3AI GATC 9 cut(s) 423, 458, 499, 826, 839, 906, 1071, 1170, 1272
Sau96I GGNCC 4 cut(s) 327, 1106, 1138, 1344
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 5 cut(s) 49, 854, 1319, 1361, 1730
ScrFI CCNGG 2 cut(s) 1132, 1480
SfaNI GCATC 4 cut(s) 317, 384, 741, 1197
SfcI CTRYAG 2 cut(s) 387, 769
Sfr274I CTCGAG 1 cut(s) 1349
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 2 cut(s) 327, 1344
SlaI CTCGAG 1 cut(s) 1349
SmiMI CAYNNNNRTG 2 cut(s) 602, 1292
SmlI CTYRAG 2 cut(s) 881, 1349
SmoI CTYRAG 2 cut(s) 881, 1349
SphI GCATGC 2 cut(s) 11, 1051
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 6 cut(s) 342, 1321, 1382, 1484, 1557, 1638
SspMI CTAG 3 cut(s) 35, 578, 758
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 1130, 1478
StyI CCWWGG 2 cut(s) 724, 934
TaaI ACNGT 4 cut(s) 243, 953, 1366, 1733
TaiI ACGT 5 cut(s) 113, 711, 1197, 1542, 1704
TaqI TCGA 8 cut(s) 114, 302, 540, 785, 891, 1100, 1163, 1350
TatI WGTACW 2 cut(s) 171, 407
TauI GCSGC 3 cut(s) 1323, 1384, 1640
TfiI GAWTC 3 cut(s) 145, 1084, 1120
Tru1I TTAA 5 cut(s) 282, 338, 359, 1257, 1652
Tru9I TTAA 5 cut(s) 282, 338, 359, 1257, 1652
TscAI CASTG 2 cut(s) 246, 973
TseFI GTSAC 2 cut(s) 1353, 1733
TseI GCWGC 8 cut(s) 11, 535, 660, 863, 899, 1051, 1418, 1438
Tsp45I GTSAC 2 cut(s) 1353, 1733
TspDTI ATGAA 6 cut(s) 213, 1053, 1073, 1106, 1328, 1563
TspGWI ACGGA 4 cut(s) 354, 533, 1228, 1581
TspRI CASTG 2 cut(s) 246, 973
Van91I CCANNNNNTGG 2 cut(s) 245, 1452
VpaK11BI GGWCC 2 cut(s) 327, 1344
XapI RAATTY 4 cut(s) 1060, 1112, 1570, 1654
XceI RCATGY 2 cut(s) 11, 1051
XhoI CTCGAG 1 cut(s) 1349
XmiI GTMKAC 1 cut(s) 912
XmnI GAANNNNTTC 1 cut(s) 1064
XspI CTAG 3 cut(s) 35, 578, 758
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.